CN112889828A

CN112889828A - Plant alkaloid algaecide and application thereof

Info

Publication number: CN112889828A
Application number: CN202110035269.3A
Authority: CN
Inventors: 魏文丰; 范德朋; 胡亚冬; 雷明科
Original assignee: Bio Form Engineering Co ltd; Beverly Biotechnology Guangdong Co ltd
Current assignee: Bio Form Engineering Co ltd; Beverly Biotechnology Guangdong Co ltd
Priority date: 2021-01-12
Filing date: 2021-01-12
Publication date: 2021-06-04

Abstract

The invention provides a plant alkaloid algicide and application thereof, which mainly comprises mimosine; when in use, the plant alkaloid is added into the water containing the blue algae according to the adding amount of 40-80mg/L for dispersion and dissolution so as to play roles of inhibiting and killing the algae. In the invention, the plant alkaloid taking mimosine as a main component is selected as the algicide, so that the method is environment-friendly, has high algae removal rate and low cost, and has economic value of large-scale popularization and utilization.

Description

Plant alkaloid algaecide and application thereof

Technical Field

The invention relates to the technical field of water treatment, and particularly relates to a plant alkaloid algaecide and application thereof.

Background

In recent years, with the rapid development of economy, a large amount of pollutants generated by human activities are discharged to natural water bodies, including industrial and agricultural wastewater, domestic sewage, excessive feed, certain metal elements or organic matters and the like, so that the environment in the water bodies is unbalanced, a large amount of nutrient salts such as nitrogen, phosphorus and the like seriously exceed the standard, and the water bodies are eutrophicated; when the temperature, the illumination and other climatic factors of the water body are in proper conditions, the cyanobacteria bloom is caused to occur frequently, and a series of abnormal responses of the ecological system, such as reduction of biological diversity, extinction of submerged plants, outbreak of the cyanobacteria bloom, and the like, are caused.

How to effectively control the growth of algae in the eutrophic water body and prevent the occurrence of the water bloom phenomenon becomes one of the research hotspots in the field of the water environment at present; scholars at home and abroad have researched a plurality of effective algae inhibiting methods, which can be summarized as follows: physical methods, chemical methods, biological methods. The methods have the advantages that in the biological method, allelochemicals generated by plants are used for inhibiting the growth of algae, so that a new thought is provided for finding a high-efficiency and safe algae inhibiting method; therefore, the research of efficient and environment-friendly algistats in allelochemicals generated by plants has wide prospect, but no related technology is found in the prior art.

Disclosure of Invention

In view of the above, the invention provides a plant alkaloid algicide, and aims to solve the problems that the existing algicide has a slow algae removal speed, is difficult to ensure the algae removal effect and has certain ecological hazard.

In a first aspect, the invention provides a plant alkaloid algicide, which mainly comprises mimosine.

Further, in the above plant alkaloid algicide, the mimosine is extracted from mimosa and/or albizia julibrissin.

In a second aspect, the invention provides a plant alkaloid algicide, which comprises the following components in parts by weight: 6-10 parts of mimosine and 0.5-3 parts of EDTA-2 Na.

Furthermore, in the plant alkaloid algaecide, the weight ratio of mimosine to EDTA-2Na is 9: 1.

In a third aspect, the invention provides a plant alkaloid algicide, which comprises the following components in parts by weight: 4-8 parts of mimosine and 2-5 parts of cinnamic acid.

Further, in the plant alkaloid algicide, the weight ratio of mimosine to cinnamic acid is 6: 4.

In a fourth aspect, the present invention further provides a method for using any one of the plant alkaloids as an algaecide, specifically: adding the plant alkaloid into the water containing the blue algae according to the preset adding amount, and dispersing and dissolving.

Further, in the method for using plant alkaloid as algaecide, the preset adding amount is 40-80 mg/L.

Further, in the method using plant alkaloid as algaecide, the blue algae is anabaena or microcystis.

The plant alkaloid algicide provided by the invention selects plant alkaloid taking mimosine as a main component as the algicide, is environment-friendly, has high algae removal rate and low cost, and has economic value of large-scale popularization and utilization.

Drawings

Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the invention. Also, like reference numerals are used to refer to like parts throughout the drawings. In the drawings:

FIG. 1 is a graph showing the change in algal density of Microcystis aeruginosa cells cultured with Mimosine at various final concentrations for 7 days in the present example;

FIG. 2 is a graph showing the mortality and algal inhibition of Microcystis aeruginosa algal cells after 7 days of incubation with Mimosine at various final concentrations in the present example.

Detailed Description

Exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. While exemplary embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art. It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict. The present invention will be described in detail below with reference to the embodiments with reference to the attached drawings.

The invention provides a plant alkaloid algicide, which mainly comprises mimosine.

In particular, alkaloids, which are one of the allelochemicals produced by plants, are a class of natural organic compounds containing nitrogen, and have a wide variety of structures and biological activities. By analyzing the growth and reproduction mechanism of algae in a water body environment and comparing the advantages and disadvantages of various algae inhibiting technologies, the function of the alkaloid compounds in the aspect of algae inhibiting has the characteristics of rapidness, easy control, small ecological hazard and the like compared with physical and chemical methods.

The mimosine selected in the embodiment of the invention can be a commercial product, and can also be extracted from mimosa and/or albizia julibrissin.

Mimosine is a nonprotein amino acid, is rich in tropical leguminous plants, such as albizia julibrissin and other leguminous plants belonging to mimosa, has wide sources, has a structure highly similar to that of thymine, and has a good algae-lysing effect. The invention provides a plant alkaloid algaecide in a second aspect, which comprises the following components in parts by weight: 6-10 parts of mimosine and 0.5-3 parts of EDTA-2Na (disodium ethylene diamine tetraacetate). Preferably, the weight ratio of mimosine to EDTA-2Na is 9: 1. EDTA-2Na is an important chelating agent, can chelate metal ions in the solution, can effectively prevent mimosine from losing activity due to the metal ions in water, can effectively reduce the content of magnesium ions in the water, can block the synthesis of algae chlorophyll a, and plays a role in assisting in algae removal.

The third aspect of the invention provides a plant alkaloid algicide, which comprises the following components in parts by weight: 4-8 parts of mimosine and 2-5 parts of cinnamic acid. Preferably, the weight ratio of mimosine to cinnamic acid is 6: 4. The cinnamic acid is an organic acid separated from cinnamon bark or benzoin, has good algae killing capacity, and can stably coexist with sensitive plants, thereby playing a role in synergistic algae dissolving.

The invention provides a method for applying the plant alkaloid as the algaecide, when in use, the plant alkaloid is added into the water body containing the blue algae according to the preset adding amount and is uniformly dispersed and dissolved in the water body. Wherein: the cyanobacteria is preferably anabaena or microcystis, and the microcystis aeruginosa is the dominant alga species of cyanobacterial bloom, can form a water bloom at the water temperature of about 30 ℃, and has good ecological significance for inhibiting the microcystis aeruginosa, so the microcystis aeruginosa in the embodiment is preferably the microcystis aeruginosa. Preferably, the dosage of the plant alkaloid is 40-80mg/L, namely the concentration of the dispersed plant alkaloid in each liter of water containing blue algae is 40-80 mg/L.

The invention is described in detail below in terms of several specific examples.

Example 1

Firstly, preparing mimosine powder into mother liquor with the concentration of 1mg/ml by using BG11 culture medium, adding the mother liquor into microcystis aeruginosa algae solution according to the adding amount with the final concentration of 40mg/L, placing the microcystis aeruginosa algae solution into an illumination incubator, wherein the culture temperature is 30 +/-1 ℃, the illumination period is 12 h: 12 h (illumination is 12 h, darkness is 12 h), the light intensity is 2000 lx, standing culture is carried out for 7 days, and the algae removal rate can reach 93.85%.

Example 2

According to the method of the embodiment 1, the addition amount of the mimosine is changed to 60mg/L, and the algae removal rate can reach 99.35%.

Example 3

According to the method of the embodiment 1, the addition amount of the mimosine is changed to 80mg/L, and the algae removal rate can reach 100%.

Example 4

According to the method of the embodiment 1, the mimosine and the EDTA-2Na are added into the anabaena in a ratio of 9:1 by mass and in a dosage of 40mg/L, and the algae removal rate can reach 95%.

Example 5

According to the method of the embodiment 1, the mimosine and the EDTA-2Na are added into the microcystis aeruginosa at a ratio of 6:0.5 by mass and 40mg/L, and the algae removal rate can reach 94%.

Example 6

According to the method of the embodiment 1, the mimosine and the EDTA-2Na are added into the microcystis aeruginosa at a ratio of 10:3 by mass and at a dosage of 40mg/L, and the algae removal rate can reach 95.65%.

Example 7

According to the method of the embodiment 1, mimosine and cinnamic acid are added into microcystis aeruginosa at a mass ratio of 6:4 and 40mg/L, and the algae removal rate can reach 94.5%.

Example 8

According to the method of the embodiment 1, mimosine and cinnamic acid are added into microcystis aeruginosa at a mass ratio of 2:1 and 40mg/L, and the algae removal rate can reach 94.95%.

Example 9

According to the method of the embodiment 1, mimosine and cinnamic acid are added into microcystis aeruginosa at a mass ratio of 8:5 and 40mg/L, and the algae removal rate can reach 94.7%.

Fig. 1 and 2 show the inhibition effect of mimosine on microcystis aeruginosa by the algicide in the embodiment of the invention, and the combination of fig. 1 and 2 shows the change trend of the cell death rate and the algae density of algae: when the addition amount of the mimosine is 80mg/L, the mimosine has the best inhibition effect on microcystis aeruginosa.

In conclusion, when the algicide provided by the invention is used for treating water containing blue-green algae, the death rate of microcystis aeruginosa can reach more than 93%, and no adverse effect is caused on the ecological environment, so that the problem of blue-green algae bloom caused by microcystis aeruginosa is solved, and the application prospect is wide.

It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.

Claims

1. A plant alkaloid algicide is characterized by mainly comprising mimosine.

2. The plant alkaloid algaecide of claim 1, wherein the mimosine is extracted from mimosa and/or albizia julibrissin.

3. A plant alkaloid algaecide is characterized by comprising the following components in parts by weight: 6-10 parts of mimosine and 0.5-3 parts of EDTA-2 Na.

4. The plant alkaloid algaecide of claim 3, wherein the weight ratio of mimosine to EDTA-2Na is 9: 1.

5. A plant alkaloid algaecide is characterized by comprising the following components in parts by weight: 4-8 parts of mimosine and 2-5 parts of cinnamic acid.

6. The plant alkaloid algicide according to claim 5, wherein the weight ratio of mimosine to cinnamic acid is 6: 4.

7. A method for using the plant alkaloid as the algaecide according to any one of claims 1 to 6, wherein the plant alkaloid is added into the water containing the blue algae according to a preset dosage and dispersed and dissolved.

8. The method as claimed in claim 7, wherein the predetermined amount is 40-80 mg/L.

9. The method of claim 7, wherein the cyanobacteria is anabaena or microcystis.