CN112881585B - Silver source detection method based on nano-enzyme catalysis driving - Google Patents
Silver source detection method based on nano-enzyme catalysis driving Download PDFInfo
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Abstract
The invention provides a silver source detection method based on nano enzyme catalysis driving, and belongs to the technical field of chemical detection. The invention takes AA-PtNPs as a nano enzyme catalyst, and the activity of the enzyme can catalyze hydrogen peroxide to decompose to generate water and oxygen; since AA-PtNPs have better chemical stability, the amount of water discharged in a fixed time is constant after adding an excessive amount of hydrogen peroxide. When Ag exists in the sample solution to be detected + Or silver nanoparticles, ag + Or silver nanoparticles, will bind to the surface of the AA-PtNPs and occupy a portion of the active sites, resulting in a significant decrease in the enzymatic activity of the AA-PtNPs, and a significant decrease in the amount of liquid discharged within a fixed time after addition of hydrogen peroxide of the same volume and concentration as described above. According to the method, the content of silver ions or silver nanoparticles in the solution of the sample to be detected is calculated according to a standard curve by calculating the difference value of the quality of the two times of liquid drainage and the linear relation between the difference value and the amount of Ag.
Description
Technical Field
The invention belongs to the technical field of chemical detection, and particularly relates to a silver source detection method based on nano-enzyme catalysis driving.
Background
Silver ion Ag + And its derivative silver nanoparticles (AgNPs) are valued for their ability to perform decorative, electrically conductive, antibacterial, and anti-inflammatory properties, and are increasingly used in consumer products (e.g., jewelry), food, and biomedical applications. However, as silver ions and silver nanoparticles become widespread in commercial applicationsHuman exposure to silver elements through ingestion, inhalation and skin presents a greater health problem. It has been reported that excessive silver intake denatures human proteins and various enzymes, causing edema of internal organs, and the like. In addition, current methods of disposal of silver-containing waste are directed to the environment, and silver has been recognized as one of the potential causes of ecological problems and damage to the ecosystem. Therefore, the accurate and rapid detection of the content of the silver ions and the silver nanoparticles has important significance for the health development of human bodies and the environmental protection.
The real-time detection method is an analysis detection method with good application prospect, and carries out analysis detection on a target object by amplifying, changing and displaying an output signal in real time. At present, the real-time detection method of silver ions or silver nanoparticles mainly comprises an ultraviolet-visible spectrophotometer test method and a fluorescence spectrum test method. However, the detection instruments of these methods are expensive, and require operators to have strong expertise, and have certain limitations in popularization and use.
Disclosure of Invention
In view of the above, the present invention aims to provide a silver source detection method based on nano-enzyme catalysis driving. The method provided by the invention is simple to operate and low in cost, and can be used for quickly and accurately detecting the content of silver ions or silver nanoparticles in a sample in real time.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides application of platinum nanoparticles in silver source detection, wherein the platinum nanoparticles are obtained by reducing chloroplatinic acid with ascorbic acid, and the silver source comprises silver ions and/or silver nanoparticles.
The invention provides a silver source detection method based on nano enzyme catalysis driving, which comprises the following steps:
(1) Mixing platinum nanoparticles with excess H 2 O 2 Mixing the solutions to perform catalytic decomposition reaction, and discharging the generated O 2 Converted to an equal volume of liquid and the mass m of discharged liquid recorded over a fixed time t 1 The platinum nanoparticles are prepared by reducing chloroplatinum by ascorbic acidObtaining acid, wherein the fixed time t is 28-32 min;
(2) Mixing platinum nanoparticles with excess H 2 O 2 Mixing the solution with the sample solution to be detected to perform catalytic decomposition reaction, and discharging the generated O 2 Converted into liquid with the same volume, and the mass m of the discharged liquid within the same fixed time t is recorded 2 (ii) a Mass of the platinum nanoparticles, H 2 O 2 The concentration and the volume of the solution are the same as those in the step (1);
(3) According to the standard curve and m 1 And m 2 Obtaining the content of the silver source in the sample solution to be detected according to the difference value delta m; the standard curve is a linear relation curve of the concentration of the silver source and the delta m;
the silver source is silver ions or silver nanoparticles;
there is no time sequence restriction between the step (1) and the step (2).
Preferably, the preparation method of the platinum nanoparticles comprises the following steps:
mixing chloroplatinic acid, ascorbic acid and water, and carrying out hydrothermal reduction reaction to obtain the platinum nanoparticles.
Preferably, the molar ratio of the chloroplatinic acid to the ascorbic acid is 1:3-4.
Preferably, the temperature of the hydrothermal reduction reaction is 60-80 ℃ and the time is 1-1.5 h.
Preferably, H in the step (1) and the step (2) 2 O 2 The concentration of the solution is 8-12 mol/L;
the platinum nano-particles are added in the form of aqueous dispersion, the concentration of the aqueous dispersion of the platinum nano-particles is 0.01-0.1 mmol/L, and the aqueous dispersion of the platinum nano-particles and H are mixed 2 O 2 The volume ratio of the solution is 1:35 to 50.
Preferably, the sample solution to be tested in the step (2) is mixed with H 2 O 2 The volume ratio of the solution is 1.
Preferably, said steps (1) and (2) are carried out at 20 to 35 ℃.
The invention provides a silver source detection device based on nano-enzyme catalytic driving, which comprises a detection container 1, wherein the detection container 1 is used for containing liquid which does not react with oxygen; the detection container 1 can be sealed;
a liquid discharge pipe 2 which can communicate the inside and the outside of the detection container 1, wherein one end of the liquid discharge pipe 2 is positioned below the liquid level in the detection container 1 when in use, and the other end is communicated with a collecting device 4 outside the detection container 1;
an open reaction vessel 3 positioned inside the detection vessel 1 and placed on the base 5; when in use, the open position of the open reaction container 3 is higher than the liquid level; during detection, platinum nanoparticles are filled in the open reaction container 3, and the platinum nanoparticles are obtained by reducing chloroplatinic acid with ascorbic acid;
and a collecting device 4 located outside the detection container 1 and communicating with the drain pipe 2.
Preferably, the liquid which does not react with oxygen is water, 0.01 to 0.05mmol/L HCl solution, 0.01 to 0.05mmol/L H 2 SO 4 One or more of the solution and 0.1-1 mmol/LNaOH solution.
The invention provides a silver source detection method based on nano-enzyme catalysis driving, platinum nano-particles (AA-PtNPs) obtained by reducing ascorbic acid are used as a nano-enzyme catalyst, and the enzyme-like activity of the AA-PtNPs can catalyze hydrogen peroxide to decompose to generate water and oxygen; since AA-PtNPs have good enzyme-like activity and chemical stability, the amount of liquid discharged in a fixed time is constant after excessive hydrogen peroxide is added. When the target object Ag exists in the sample solution to be detected + Or silver nanoparticles, ag + Or silver nanoparticles, will bind to the surface of the AA-PtNPs and occupy a portion of the active sites on the surface of the AA-PtNPs, resulting in a significant decrease in the enzymatic activity of the AA-PtNPs, and a significant decrease in the amount of liquid discharged within the same fixed time period after addition of the same concentration and volume of hydrogen peroxide. According to the method, the content of silver ions or silver nanoparticles in the solution of the sample to be detected is calculated according to the standard curve by calculating the difference of the quality of the two times of liquid drainage and the linear relation between the variable quantity and the concentration of Ag. In the invention, the platinum nanoparticles obtained by reducing chloroplatinic acid with ascorbic acid have negative charges on the surface, silver ions have positive charges, and the platinum is enabled to be under the action of the positive charges and the negative chargesSilver ions are easier to be enriched around the nano material, and the detection accuracy can be improved. In the invention, the signal output mode is to weigh the mass of the discharged liquid, thereby avoiding the limitation of precise instruments and equipment and professional operators required for analysis and detection. Meanwhile, AA-PtNPs are on Ag + Or the silver nanoparticles have good selectivity, and can avoid the interference of other impurity ions, thereby ensuring the accuracy of the measurement result.
The invention provides a silver source detection device based on nano-enzyme catalysis driving, which comprises a detection container 1 filled with liquid which does not react with oxygen, an open reaction container 3 positioned in the detection container 1 and placed on a base 5, and a collection device 4 positioned outside the detection container 1 and connected with a liquid discharge pipe 2. The device provided by the invention can simply, conveniently and quickly detect the content of silver ions or silver nanoparticles in a sample in real time, has a simple structure and low cost, and is suitable for popularization and use.
Drawings
FIG. 1 is a schematic structural diagram of a silver source detection device based on nano-enzyme catalytic driving, wherein 1 is a detection container, 2 is a liquid discharge pipe, 3 is an open reaction container, 4 is a collection device, and 5 is a base;
FIG. 2 is a graph showing the variation of silver ion concentration with respect to Δ m;
FIG. 3 is a standard curve of silver ion concentration versus Δ M in the range of 0 to 0.3 μ M;
FIG. 4 is a graph of the variation of silver nanoparticle concentration with Δ m;
FIG. 5 is a standard graph of silver nanoparticle concentration versus Δ m from 0 to 350 pM;
FIG. 6 shows m of different interfering ions 2 /m 1 Compare the figures.
Detailed Description
The invention provides application of platinum nanoparticles in silver source detection, wherein the platinum nanoparticles are obtained by reducing chloroplatinic acid with ascorbic acid, and the silver source comprises silver ions and/or silver nanoparticles.
The invention provides a silver source detection method based on nano enzyme catalysis driving, which comprises the following steps:
(1) Mixing platinum nanoparticles with excess H 2 O 2 Mixing the solutions to perform catalytic decomposition reaction, and discharging the generated O 2 Converted to an equal volume of liquid and the mass m of discharged liquid recorded over a fixed time t 1 The platinum nanoparticles are obtained by reducing chloroplatinic acid with ascorbic acid, and the fixed time t is 30min;
(2) Mixing platinum nanoparticles with excess H 2 O 2 Mixing the solution with the sample solution to be detected to perform catalytic decomposition reaction, and discharging the generated O 2 Converting into liquid with equal volume, and recording the mass m of discharged liquid within the same fixed time t 2 (ii) a Mass of the platinum nanoparticles, H 2 O 2 The concentration and volume of the solution are the same as those in the step (1);
(3) According to the standard curve and m 1 And m 2 Obtaining the content of the silver source in the sample solution to be detected according to the difference value delta m; the standard curve is a linear relation curve of the concentration of the silver source and the delta m;
the silver source is silver ions or silver nanoparticles;
there is no time sequence restriction between the step (1) and the step (2).
The invention combines platinum nanoparticles and excess H 2 O 2 Mixing the solutions to perform catalytic decomposition reaction, and discharging the generated O 2 Converted to an equal volume of liquid and the mass m of discharged liquid recorded over a fixed time t 1 The platinum nanoparticles are obtained by reducing chloroplatinic acid with ascorbic acid, and the fixed time t is 30min. The method for preparing the platinum nanoparticles in the present invention preferably comprises the steps of:
mixing chloroplatinic acid, ascorbic acid and water, and carrying out hydrothermal reaction to obtain the platinum nanoparticles.
In the present invention, the molar ratio of chloroplatinic acid to ascorbic acid is preferably 1:3 to 4, more preferably 1; in the present invention, the temperature of the hydrothermal reaction is preferably 60 to 80 ℃, more preferably 60 to 70 ℃, and the time is preferably 1 to 1.5 hours.
After the hydrothermal reaction, the present invention preferably performs a post-treatment on the obtained hydrothermal reaction solution, and the post-treatment preferably includes the following steps:
and carrying out centrifugal washing on the hydrothermal reaction liquid to obtain the platinum nano-particles.
In the invention, the speed of the centrifugal washing is preferably 15000r/min, and the time is preferably 10-15 min; the detergent for centrifugal washing is preferably water, the temperature of the centrifugal washing is preferably 4 ℃, and the number of times of the centrifugal washing is preferably 2.
In the present invention, said H 2 O 2 The concentration of the solution is preferably 10mol/L; the platinum nanoparticles are added in the form of an aqueous dispersion, and the concentration of the aqueous dispersion of the platinum nanoparticles is preferably 0.01-0.1 mmol/L, and more preferably 0.05mmol/L; of the aqueous dispersion of platinum nanoparticles with H 2 O 2 The volume ratio of the solution is preferably 1. In the present invention, the fixed time t is preferably 28 to 32min, and more preferably 30min.
To obtain m 1 Then, the invention adds platinum nanoparticles and excess H 2 O 2 Mixing the solution with the sample solution to be detected to perform catalytic decomposition reaction, and discharging the generated O 2 Converted into liquid with the same volume, and the mass m of the discharged liquid within the same fixed time t is recorded 2 (ii) a Mass of the platinum nanoparticles, H 2 O 2 The concentration and volume of the solution are the same as in step (1).
In the invention, when the solution of the sample to be tested contains silver ions, the content of the silver ions in the sample to be tested is preferably 0-0.3 μ M; when the sample to be detected contains silver nanoparticles, the content of the silver nanoparticles in the sample to be detected is preferably 0 to 350pM.
To obtain m 2 Then, the invention is based on the standard curve and m 1 And m 2 Obtaining the content of silver ions or silver nanoparticles in the solution of the sample to be detected; the standard curve is a linear relation curve of the concentration of the silver source in the silver source solution and the delta m.
In the present invention, when the standard curve is a linear relationship curve of silver ion concentration and Δ m, the method for plotting the standard curve preferably comprises the following steps:
(1) Providing a silver ion standard solution;
(2) The method is adopted to obtain the Delta m of the silver ion standard solution, the concentration of the silver ion standard solution is used as a horizontal coordinate, and the Delta m is used as a vertical coordinate to draw a standard curve.
In the invention, the solute in the silver ion standard solution is preferably silver nitrate; the concentration of the silver ion standard solution is preferably 0nM, 5nM, 10nM, 25nM, 50nM, 100nM, 175nM, 250nM, 500nM, 1. Mu.M, 2.5. Mu.M, 3.5. Mu.M and 5. Mu.M. As a specific embodiment of the invention, the standard curve of the silver ion concentration and Δ m is Δ m = -3.2827C +1.8318 2 =0.9984; wherein C is silver ion concentration, μ M.
In the present invention, when the standard curve is a linear relationship curve of silver nanoparticle concentration and Δ m, the method for plotting the standard curve preferably comprises the steps of:
(1) Providing a standard solution of silver nanoparticles;
(2) The method is adopted to obtain the Delta m of the silver nanoparticle standard solution, the concentration of the silver nanoparticle standard solution is used as a horizontal coordinate, and the Delta m is used as a vertical coordinate to draw a standard curve.
In the present invention, the particle size of the silver nanoparticles is preferably 10 to 100nm, and as a specific example of the present invention, the particle size of the silver nanoparticles is 30 to 50nm; the concentration of the silver nanoparticle standard solution is preferably 0pM, 10pM, 20pM, 50pM, 100pM, 150pM, 200pM, 300pM and 500pM. As one specific example of the invention, the standard curve of the silver nanoparticle concentration and Δ m is Δ m = -0.0055C +1.8141 2 =0.9977; where C is the silver nanoparticle concentration, pM.
The invention provides a silver ion or silver nanoparticle detection device based on nano-enzyme catalysis driving, which comprises a detection container 1, wherein the detection container 1 is used for containing liquid which does not react with oxygen; the detection container 1 can be sealed;
a liquid discharge pipe 2 which can communicate the inside and the outside of the detection container 1, wherein one end of the liquid discharge pipe 2 is positioned below the liquid level in the detection container 1 when in use, and the other end is communicated with a collecting device 4 outside the detection container 1;
an open reaction vessel 3 positioned inside the detection vessel 1 and placed on the base 5; when in use, the open position of the open reaction container 3 is higher than the liquid level; during detection, platinum nanoparticles are filled in the open reaction container 3, and the platinum nanoparticles are obtained by reducing chloroplatinic acid with ascorbic acid;
and a collecting device 4 located outside the detection container 1 and communicating with the drain pipe 2.
The invention provides a device for detecting silver ions or silver nanoparticles based on nano-enzyme catalytic driving, which comprises a detection container 1. In the present invention, the detection container 1 is used for containing liquid which does not react with oxygen; the detection container 1 can be sealed. The present invention does not require any particular kind of detection container 1, and a sealable container known to those skilled in the art may be used. As an embodiment of the present invention, the detection container 1 is preferably a glass bottle with a bottle stopper. The invention has no special requirements on the size and specification of the detection container 1, and can be designed correspondingly according to the actual situation.
In the present invention, the liquid which does not react with oxygen is preferably water, 0.01 to 0.05mmol/L HCl solution, 0.01 to 0.05mmol/L H 2 SO 4 One or more of the solution and 0.1-1 mmol/L NaOH solution.
The silver ion or silver nanoparticle detection device based on nano-enzyme catalytic driving comprises a liquid discharge pipe 2 capable of communicating the inside and the outside of a detection container 1, wherein one end of the liquid discharge pipe 2 is positioned below the liquid level when the device is used, and the other end of the liquid discharge pipe 2 is communicated with the outside of the detection container 1. The invention has no special requirements on the type and the material of the liquid discharge pipe 2, can realize the liquid discharge function and does not react with liquid.
The invention provides a silver ion or silver nanoparticle detection device based on nano-enzyme catalysis driving, which comprises an open reaction container 3, a detection container 1 and a base 5, wherein the open reaction container is positioned inside the detection container; the open position of the open reaction vessel 3 is higher than the liquid level. The invention does not require any special kind of open reaction vessel 3, and open vessels known to those skilled in the art, such as beakers, can be used. The invention has no special requirements on the size and specification of the open reaction vessel 3, and can be designed correspondingly according to actual conditions.
In the present invention, in the detection, platinum nanoparticles obtained by reducing chloroplatinic acid with ascorbic acid are filled in the open reaction vessel 3.
The device for detecting silver ions or silver nanoparticles based on the catalytic driving of the nanoenzyme provided by the invention also preferably comprises a base 5 positioned at the bottom in the detection container 1, wherein the base 5 is used for placing the open reaction container 3 to increase the height of the open reaction container 3 in the detection container 1.
The invention provides a device for detecting silver ions or silver nanoparticles based on nano-enzyme catalytic driving, which comprises a collecting device 4 which is positioned outside a detection container 1 and connected with a liquid discharge pipe 2. The invention does not require a special device for the handset, and uses a collection container known to those skilled in the art.
The structure schematic diagram of the silver ion or silver nanoparticle detection device based on nano-enzyme catalytic driving is shown in figure 1, wherein 1 represents a detection container, 2 represents a liquid discharge pipe, 3 represents an open reaction container, 4 represents a collection device, and 5 represents a base.
The following examples are provided to illustrate the method for detecting silver source based on nanoenzyme catalytic driving of the present invention in detail, but they should not be construed as limiting the scope of the present invention.
Example 1
The preparation method of the silver source detection device based on the nano-enzyme catalysis driving comprises the following steps:
(1) A drilling machine is used for drilling a small hole with the diameter of 1mm on the glass bottle, and the position of the small hole is 3cm away from the bottom of the glass bottle and is higher than the upper end of an open reaction container in the glass bottle. A glass capillary tube having a length of 5cm, an inner diameter of 0.9mm and an outer diameter of 1.1mm was cut out, and the diameter of the glass capillary tube was measured at 3: and 2, marking by using an oiliness pen, horizontally holding one end of the glass capillary tube, slightly contacting by using a tiny open fire, slightly burning, and immediately stopping burning after an included angle of 90 degrees is formed at the left end and the right end of the marked position of the glass capillary tube. Inserting one end of the manufactured glass capillary tube with the bending angle of 90 degrees by 3cm into a small hole of the glass bottle body, and enabling the end to be parallel to the bottle body; one end of 2cm is vertical to the bottle body. And then, adhering the contact point of the glass capillary tube and the bottle body by using 10 mu L of 502 glue, after the adhesion is stable, dropwise adding a mixture of common glue and 502 glue to block the rest position of the hole, and drying for 3 hours at normal temperature.
(2) A section of rigid material with the height of 1cm and the length and the width of 0.2cm is cut out to manufacture a base, and then a tube cover of a 2mL centrifuge tube is cut out, and all the materials except a central groove of the cover are cut out. Vertically placing the base, adhering the cut centrifugal tube cover to the upper end of the base by using glue, wherein one surface of the groove faces upwards; after glue is coated on the lower end of the base, the base is vertically placed into a glass bottle, the lower end of the base is vertically adhered to the bottom of the glass bottle, and the base is dried for 3 hours at normal temperature.
(3) The sealing film is wound on the bottle mouth of the glass bottle for two circles, so that the sealing property of the glass bottle is ensured.
(4) Verifying the tightness of the experimental instrument, injecting 3mL of ultrapure water solution into the prepared glass bottle, and screwing down the cover of the glass bottle to extrude oxygen in the glass bottle to increase the air pressure. At this time, a small amount of the aqueous solution was discharged from the tube, and the tube was filled with the aqueous solution for a long time after the air pressure in the bottle was stabilized, so that the suck-back phenomenon was not caused, indicating that the sealing property of the glass bottle was good.
Example 2
Preparation of platinum nanoparticles:
(1) Adding 2mL of chloroplatinic acid hexahydrate with the concentration of 2mmol/L and 1mL of ultrapure water into a reaction vessel, heating to 60 ℃ under magnetic stirring, adding 1.5mL of ascorbic acid with the concentration of 10mmol/L, heating and stirring for 1 hour at 60 ℃, and changing the solution from light yellow to brown and then to dark brown in the hydrothermal reaction process;
(2) After the hydrothermal reaction is finished, the reaction liquid is cooled to room temperature, and is centrifugally washed twice at 15000r/min to obtain the platinum nano-particles AA-PtNPs, and the obtained AA-PtNPs are re-dispersed in water and stored at 4 ℃.
Example 3
Standard curve of silver ion concentration versus Δ m:
(1) To the open reaction vessel of example 1 were added 5. Mu.L of 0.05mmol/L AA-PtNPs and 200. Mu.L of 10mol/L H 2 O 2 The solution, closed glass bottle, recorded the mass m of the liquid discharged within 30min 1 ；
(2) Adding AA-PtNPs and H into the open reaction vessel again 2 O 2 Solutions and silver ion standard solutions of different concentrations (0 nM, 5nM, 10nM, 25nM, 50nM, 100nM, 175nM, 250nM, 500nM, 1. Mu.M, 2.5. Mu.M, 3.5. Mu.M, 5. Mu.M and 10. Mu.M), closed test vessel 1, recording the mass M of discharged liquid within 30min 2 The AA-PtNPs and H 2 O 2 The amount of the solution added was the same as in step (1). The curve of the silver ion concentration versus Δ m is shown in fig. 2. As can be seen from FIG. 2, the silver ion concentration and Δ M are linearly and negatively correlated in the range of 0 to 0.3 μ M, and a standard curve is plotted with the silver ion standard solution concentration as abscissa and Δ M as ordinate in the range of 0 to 0.3 μ M, and the results are shown in FIG. 3. Wherein, the standard curve of the silver ion concentration and the delta m is that the delta m is = -3.2827C +1.8318 2 =0.9984; c is silver ion concentration, mu M, and C is between 0 and 0.3 mu M.
Standard curve of silver nanoparticle concentration versus Δ m:
(1) 5 μ L of 0.05mmol/L AA-Pt NPs and 200 μ L of 10mol/L H were added to the open reaction vessel of example 1 2 O 2 The solution, closed glass bottle, record the mass m of liquid discharged within 30min 1 ；
(2) Adding AA-PtNPs and H into the open reaction vessel again 2 O 2 Solutions and silver nanoparticle standard solutions of different concentrations (0 pM, 10pM, 20pM, 50pM, 100pM, 150pM, 200pM, 300pM, 500pM, 1000pM and 1500 pM), sealing the detection container 1, and recording the mass m of discharged liquid within 30min 2 The AA-PtNPs and H 2 O 2 The amount of the solution added was the same as in step (1). The variation of silver nanoparticle concentration versus Δ m is shown in fig. 4. As can be seen from FIG. 4, the silver ion concentration is linearly and negatively correlated with Δ m in the range of 0 to 350pM, and the concentration of the silver nanoparticle standard solution is measured in the range of 0 to 350pMThe results are shown in FIG. 5, where the abscissa represents Δ m and the ordinate represents Δ m. Wherein the standard curve of the silver nanoparticle concentration and the delta m is that the delta m is = -0.0055C +1.8141 2 =0.9977; where C is the silver nanoparticle concentration, pM.
Example 4
Silver ion (Ag) in tap water + ) The labeling recovery experiment of (1):
(1) 5. Mu.L of 0.05mmol/L AA-PtNPs and 200. Mu.L of 10mol/L H were added to the open reaction vessel of example 1 2 O 2 The solution, closed glass bottle, record the mass m of liquid discharged within 30min 1 ；
(2) Placing tap water solution at room temperature for 10min, and preparing Ag (5 nM, 50nM, 100 nM) with tap water solution + 。
(3) Taking 50 mu L of Ag in the step (2) + The solution was mixed with the same volume of 0.1mmol/L AA-Pt NPs and incubated for 5min.
(4) Transferring 5. Mu.L of the mixed sample obtained in step (3) into a reaction vessel, and adding 200. Mu.L of 10mol/L H 2 O 2 Sealing the glass bottle, recording the mass m of the discharged liquid within 30min 2 ；
(5) The concentration of silver ions was calculated by substituting Δ m into the standard curve of silver ion concentration and Δ m in example 3. The recovery and RSD were calculated by the ratio of the actual calculated concentration to the theoretical concentration. The results obtained are shown in table 1.
TABLE 1 experimental results of silver ion recovery from tap water
Example 5
Spiking recovery experiment of silver nanoparticles (AgNPs) in tap water:
(1) 5. Mu.L of 0.05mmol/L AA-PtNPs and 200. Mu.L of 10mol/L H were added to the open reaction vessel of example 1 2 O 2 The solution, closed glass bottle, record the mass m of liquid discharged within 30min 1 ；
(2) Tap water solution was allowed to stand at room temperature for 10min, and tap water solution was used to prepare AgNPs of various concentrations (10 pM, 100pM, 1000 pM).
(3) Adding 1 mu L of 10mol/L H into the AgNPs solution in the step (2) 2 O 2 And when the mixture is mixed for 1min, the AgNPs are all converted into Ag + 。
(4) And (3) adding 50 mu L of AgNPs solution in the step (3) into the same volume of 0.1mmol/L platinum nanoparticle solution, and mixing and incubating for 5min.
(5) Transferring 5. Mu.L of the mixed sample obtained in the step (4) into a reaction vessel, and adding 200. Mu.L of 10mol/L H 2 O 2 Sealing the glass bottle, recording the mass m of the discharged liquid within 30min 2 ；
(6) The concentration of silver nanoparticles was calculated by substituting Δ m into the standard curve of silver nanoparticle concentration and Δ m in example 3. The recovery and RSD were calculated by the ratio of the actual calculated concentration to the theoretical concentration. The results are shown in Table 2.
TABLE 2 spiking recovery experimental data for silver nanoparticles in tap water
Example 6
(1) 5. Mu.L of 0.05mmol/L AA-PtNPs and 200. Mu.L of 10mol/L H were added to the open reaction vessel of example 1 2 O 2 The solution, closed glass bottle, record the mass m of liquid discharged within 30min 1 ；
(2) Re-adding the same amount of AA-PtNPs and H into the open reaction vessel 2 O 2 Adding silver ion standard solution with concentration of 500nM and different interfering ion solutions, respectively, wherein the interfering ions are sodium ions (Na) + ) Potassium ion (K) + ) Magnesium ion (Mg) 2+ ) Manganese ion (Mn) 2+ ) Zinc ion (Zn) 2+ ) Cadmium ion (Cd) 2+ ) Ferrous ion (Fe) 2+ ) Iron ion (Fe) 3+ ) Chromium ion (Cr) 3+ ) Lithium ion (Li) + ) Acetate ion (Ac) - ) Fluorine ion (F) - ) Bromine ion (Br) - ) Barium ion (Ba) 2+ )、Ammonium ion (NH) 4 + ) Nickel ion (Ni) 2+ ) Chloride ion (Cl) - ) Sulfate ion (SO) 4 2- ) Nitrate ion (NO) 3 - ) Carbonate ion (CO) 3 2- ) Sulfite ion (SO) 3 2- ) The concentration of interfering ions was 5 μ M and the mass M of liquid discharged within 30min was recorded 2 ；
(3) Calculate m 2 And m 1 The results are shown in FIG. 6. As can be seen from FIG. 6, the quality of the second drainage was significantly reduced in the presence of 500nM of the silver ion standard solution, m of which 2 /m 1 40.5%, and in the presence of other interfering ions, the quality of the second drainage did not significantly decrease, its m 2 /m 1 All are about 100%, which shows that AA-PtNPs are applied to Ag + Has good selectivity and can avoid the interference of other impurity ions.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (7)
1. A silver source detection method based on nano-enzyme catalysis driving comprises the following steps:
(1) Mixing platinum nanoparticles with an excess of H 2 O 2 Mixing the solutions to perform catalytic decomposition reaction, and discharging the generated O 2 Converted to an equal volume of liquid and the mass m of discharged liquid recorded over a fixed time t 1 The platinum nano particles are obtained by reducing chloroplatinic acid with ascorbic acid, and the fixed time t is 28-32 min;
(2) Mixing platinum nanoparticles with excess H 2 O 2 Mixing the solution with the sample solution to be detected to perform catalytic decomposition reaction, and discharging the generated O 2 Converting into liquid with equal volume, and recording the mass m of discharged liquid within the same fixed time t 2 (ii) a Mass of the platinum nanoparticles, H 2 O 2 Concentration of the solutionThe degree and the volume are the same as those in the step (1);
(3) According to the standard curve and m 1 And m 2 Obtaining the content of the silver source in the sample solution to be detected; the standard curve is a linear relation curve of the concentration of the silver source and the delta m;
the silver source is silver ions or silver nanoparticles;
no time sequence limitation exists between the step (1) and the step (2);
the preparation method of the platinum nanoparticles comprises the following steps:
mixing chloroplatinic acid, ascorbic acid and water, and carrying out hydrothermal reduction reaction to obtain platinum nanoparticles;
the molar ratio of the chloroplatinic acid to the ascorbic acid is 1:3-4.
2. The silver source detection method according to claim 1, wherein the temperature of the hydrothermal reduction reaction is 60 to 80 ℃ and the time is 1 to 1.5 hours.
3. The silver source detection method according to claim 1, wherein H is used in the step (1) and the step (2) 2 O 2 The concentration of the solution is 8-12 mol/L;
the platinum nano-particles are added in the form of aqueous dispersion, the concentration of the aqueous dispersion of the platinum nano-particles is 0.01-0.1 mmol/L, and the aqueous dispersion of the platinum nano-particles and H are mixed 2 O 2 The volume ratio of the solution is 1:35 to 50 percent.
4. The method for detecting silver source according to claim 3, wherein the sample solution to be tested and H in the step (2) 2 O 2 The volume ratio of the solution is 1.
5. The method for detecting silver source according to claim 1, wherein the steps (1) and (2) are performed at 20 to 35 ℃.
6. A silver source detection device based on nano-enzyme catalysis driving comprises a detection container (1), wherein the detection container (1) is used for containing liquid which does not react with oxygen; the detection container (1) can be closed;
the liquid discharge pipe (2) can be communicated with the inside and the outside of the detection container (1), one end of the liquid discharge pipe (2) is positioned below the liquid level in the detection container (1) when the liquid discharge pipe is used, and the other end of the liquid discharge pipe is communicated with a collecting device (4) outside the detection container (1);
an open reaction vessel (3) which is positioned in the detection vessel (1) and is placed on the base (5); when in use, the open position of the open reaction container (3) is higher than the liquid level; when detection is carried out, platinum nano-particles are filled in the open reaction container (3), and the platinum nano-particles are obtained by reducing chloroplatinic acid by ascorbic acid;
and a collecting device (4) which is positioned outside the detection container (1) and is communicated with the liquid discharge pipe (2).
7. The silver source detection device of claim 6, wherein the liquid that does not react with oxygen is water, 0.01 to 0.05mmol/L HCl solution, 0.01 to 0.05mmol/L H 2 SO 4 One or more of the solution and 0.1-1 mmol/L NaOH solution.
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