CN112852976A - Molecular marker related to later-stage egg laying traits in laying hen NCS1 gene and application thereof - Google Patents
Molecular marker related to later-stage egg laying traits in laying hen NCS1 gene and application thereof Download PDFInfo
- Publication number
- CN112852976A CN112852976A CN202110285978.7A CN202110285978A CN112852976A CN 112852976 A CN112852976 A CN 112852976A CN 202110285978 A CN202110285978 A CN 202110285978A CN 112852976 A CN112852976 A CN 112852976A
- Authority
- CN
- China
- Prior art keywords
- laying
- later
- haplotype
- molecular marker
- traits
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000017448 oviposition Effects 0.000 title claims abstract description 38
- 239000003147 molecular marker Substances 0.000 title claims abstract description 32
- 101150074759 NCS1 gene Proteins 0.000 title claims abstract description 24
- 102000054766 genetic haplotypes Human genes 0.000 claims abstract description 70
- 238000000034 method Methods 0.000 claims abstract description 10
- 239000002773 nucleotide Substances 0.000 claims abstract description 10
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 10
- 238000009395 breeding Methods 0.000 claims abstract description 9
- 238000001514 detection method Methods 0.000 claims description 12
- 101100236128 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) LSM2 gene Proteins 0.000 claims description 11
- 101000690100 Homo sapiens U1 small nuclear ribonucleoprotein 70 kDa Proteins 0.000 claims description 10
- 102100024121 U1 small nuclear ribonucleoprotein 70 kDa Human genes 0.000 claims description 10
- 108700028369 Alleles Proteins 0.000 claims description 8
- 101100029173 Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) SNP2 gene Proteins 0.000 claims description 5
- 101100094821 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SMX2 gene Proteins 0.000 claims description 5
- 239000008280 blood Substances 0.000 claims description 4
- 210000004369 blood Anatomy 0.000 claims description 4
- 238000011144 upstream manufacturing Methods 0.000 claims description 3
- 230000035772 mutation Effects 0.000 abstract description 13
- 239000003550 marker Substances 0.000 abstract description 11
- 230000001488 breeding effect Effects 0.000 abstract description 7
- 238000012216 screening Methods 0.000 abstract description 6
- 108091026890 Coding region Proteins 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 108020004414 DNA Proteins 0.000 description 13
- 241000287828 Gallus gallus Species 0.000 description 13
- 235000013330 chicken meat Nutrition 0.000 description 13
- 238000004458 analytical method Methods 0.000 description 9
- 235000013601 eggs Nutrition 0.000 description 8
- 239000012634 fragment Substances 0.000 description 7
- 238000012408 PCR amplification Methods 0.000 description 6
- 238000012098 association analyses Methods 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 101100459913 Gallus gallus NCS1 gene Proteins 0.000 description 5
- 238000010219 correlation analysis Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000012163 sequencing technique Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 102100032077 Neuronal calcium sensor 1 Human genes 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 244000144992 flock Species 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 101000637977 Homo sapiens Neuronal calcium sensor 1 Proteins 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 101001083117 Microbacterium liquefaciens Hydantoin permease Proteins 0.000 description 3
- 238000009826 distribution Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 238000012097 association analysis method Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 102000054765 polymorphisms of proteins Human genes 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 235000013594 poultry meat Nutrition 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 101100459912 Caenorhabditis elegans ncs-1 gene Proteins 0.000 description 1
- 102000005701 Calcium-Binding Proteins Human genes 0.000 description 1
- 108010045403 Calcium-Binding Proteins Proteins 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000029726 Neurodevelopmental disease Diseases 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000003919 adipocyte function Effects 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 230000036750 egg laying performance Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000028023 exocytosis Effects 0.000 description 1
- 230000003325 follicular Effects 0.000 description 1
- 230000008217 follicular development Effects 0.000 description 1
- 108010062994 frequenin calcium sensor proteins Proteins 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 229940067606 lecithin Drugs 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000009401 metastasis Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 230000004770 neurodegeneration Effects 0.000 description 1
- 208000015122 neurodegenerative disease Diseases 0.000 description 1
- 230000001123 neurodevelopmental effect Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000008279 sol Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 208000001072 type 2 diabetes mellitus Diseases 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/124—Animal traits, i.e. production traits, including athletic performance or the like
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/172—Haplotypes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a molecular marker related to later-stage egg-laying traits in an NCS1 gene of laying hens and application thereof, wherein the molecular marker is positioned at a partial coding region of the NCS1 gene, the nucleotide sequence of the molecular marker is shown as SEQ ID NO:1, the molecular marker totally comprises 3 SNP loci, namely three allelic mutation loci of C/T, C/G and A/G exist at the 171bp, 482bp and 567bp respectively, and a haplotype combination formed by the three allelic mutation loci can be used as a haplotype molecular marker related to the later-stage egg-laying traits of the laying hens, wherein the haplotype combination is H1H 3: the egg laying number of an individual CCG/CGG at 60 weeks is obviously higher than that of other haplotype combinations, and the selection of the haplotype combination is beneficial to improving the whole later egg laying number of the laying hen, namely, the invention provides a new molecular breeding marker for marker-assisted breeding of the later egg laying character of the laying hen, realizes the screening of the later egg laying character of the laying hen, and the screening method is simple and quick.
Description
Technical Field
The invention relates to the technical field of laying hen genetic marker screening, in particular to a molecular marker related to later-stage egg laying traits in an laying hen NCS1 gene and application thereof.
Background
The eggs are rich in various nutrient substances needed by human bodies, such as protein, fat, lecithin and the like, and are important components in the dietary structure of residents in China. The later egg laying period is a period in which the production performance of the chicken flocks is continuously reduced, the later egg laying period accounts for 50% of the whole egg laying period, the chicken flocks of about 500 days old are generally eliminated in breeding production, but the laying rate of the chicken flocks is still maintained at 60-70% at the moment, and the production performance of the chicken flocks in the later egg laying period directly influences the economic benefit of the laying hen breeding. The main reasons for the decrease of laying rate of laying hens in the later period of laying are ovary aging and hypofunction in the later period of laying hens, and the decrease of the number of follicles, the increase of dominant follicular atresia rate of development, the inhibition of follicular development and the like are mainly shown, so that the utilization period of the laying hens in the later period of laying hens is shortened, the economic value is reduced, and therefore, the increase of the laying rate of the laying hens in the later period of laying hens has important significance for the laying hens.
Single Nucleotide Polymorphism (SNP) refers to a polymorphism in a genomic DNA sequence caused by mutation of a Single Nucleotide (A, T, C or G), and includes insertion, deletion, transformation, transversion and the like of a Single base. genome-Wide Association Study (GWAS) is a genome-Wide Association analysis that uses millions of SNPs in a genome as molecular genetic markers to perform genome-Wide level control analysis and correlation analysis. The emergence of a large number of SNP markers gradually transformed the association analysis method centered on a single marker into the association analysis method based on haplotype (haplotype). Haplotypes are the SNPs that are tightly linked and that determine the same trait on the same chromosome or in a certain region, and are statistically related. The SNP Marker and the haplotype composed of the SNP Marker play an important role in the breeding of animal molecular Marker Assisted Selection (MAS) due to the distribution universality and stability, and become feasible technologies for the genetic improvement of the later-stage characters, especially the restrictive characters of the laying hens. Based on the method, SNP markers and haplotype combinations thereof related to the economic traits of the laying hens are further identified, and then the SNP markers and the haplotype combination sequences thereof are used as screening markers for auxiliary selection of the economic traits of the excellent laying hens, so that the accuracy of seed selection is greatly improved, and the method has certain significance for cultivating the laying hen varieties with the excellent economic traits.
Neuronal Calcium Sensor 1 (NCS 1) is a highly conserved Calcium binding protein involved in a variety of physiological cellular functions, including exocytosis, regulation of Calcium-permeable channels, neuroplasticity, and response to Neuronal injury. NCS1 has been shown to play an important role in adipocyte function, and a deficiency in NCS1 has been shown to contribute to obesity and type 2 diabetes in adult mice; altered expression of NCS1 is associated with neurodevelopmental and neurodegenerative diseases; tumor invasiveness can be promoted by promoting cell survival and metastasis, and the like. However, no report is found about the research of the NCS1 gene on the laying hens, and no research about the NCS1 gene of the laying hens as a molecular marker of the laying performance of the laying hens in the later period is available so far.
Disclosure of Invention
The invention aims to provide a molecular marker related to later-stage egg laying traits in an NCS1 gene of a laying hen and application thereof, and discovers a haplotype molecular marker related to later-stage egg laying performance in a partial coding region of the NCS1 gene of the laying hen for the first time, thereby providing an important reference basis for auxiliary selection of the later-stage egg laying traits of the laying hen.
One purpose of the invention is to provide a molecular marker related to later laying traits in an egg chicken NCS1 gene, wherein the nucleotide sequence of the molecular marker is shown in a sequence table SEQ ID NO. 1, and the molecular marker comprises:
SNP1 site: C/T polymorphic sites exist at the 171bp position in the sequence shown in SEQ ID NO. 1;
SNP2 site: the C/G polymorphic site exists at the 482bp position in the sequence shown in SEQ ID NO. 1;
SNP3 site: the A/G polymorphic site exists at the 567bp position in the sequence shown in SEQ ID NO. 1.
Further, the dominant alleles at 171bp, 482bp and 567bp in the sequence are C, C, G respectively, and the dominant genotypes are CC, CC and GG respectively.
Another object of the present invention is to provide a primer set for amplifying the molecular marker, the primer set comprising: the nucleotide sequence of the upstream primer is shown as SEQ ID NO. 2, and the nucleotide sequence of the downstream primer is shown as SEQ ID NO. 3.
The invention also aims to provide a detection method of the molecular marker related to the later laying character in the NCS1 gene of the laying hen, which specifically comprises the following steps: and (3) detecting by using the primer pair.
Further, the detection method comprises the following steps:
extracting genome DNA from blood of laying hens, amplifying by using the primer pair of claim 3 by using the genome DNA as a template, and comparing the sequence of the amplified products to obtain 3 SNP sites as described in claim 1.
The fourth object of the present invention is to provide a haplotype combination comprising the SNP sites, wherein the haplotype combination comprises the combination of SNP1-SNP3 sites.
The fifth objective of the present invention is to provide a method for constructing a haplotype combination, which comprises: firstly, detecting 3 SNP sites by adopting the detection method of the molecular marker; six haplotypes were then constructed, H1: CCG, H2: CCA, H3: CGG, H4: CGA, H5: TGG, H6: TGA; and then, establishing a haplotype combination according to the haplotype, and correlating the haplotype combination with the later laying traits of the laying hens.
The sixth purpose of the invention is to provide the application of the molecular marker, the primer pair or the haplotype combination in the auxiliary selection of the laying characteristics of the laying hens in the later period.
Further, the later-period egg laying traits of the laying hens comprise the egg laying number of 40 weeks and/or the egg laying number of 60 weeks
The seventh purpose of the invention is to provide a breeding method of late-stage high-yield laying hens, which comprises the following steps: the haplotype combination is constructed by adopting the construction method of the haplotype combination, and the haplotype combination is kept as HIH 3: individuals of CCG/CGG.
Compared with the prior art, the invention has the beneficial effects that: the invention firstly discovers a haplotype molecular marker related to the later laying performance of the laying hen in part of the coding region of the NCS1 gene of the laying hen, the sequence of the haplotype molecular marker is shown as SEQ ID NO:1, the length of the sequence is 1578bp, the sequence totally comprises 3 SNP sites, namely three allelic mutation sites of C/T, C/G and A/G exist at the 171bp, 482bp and 567bp respectively, and a haplotype combination formed by the haplotype marker can be used as the haplotype molecular marker related to the later laying character of the laying hen, wherein the haplotype combination H1H 3: the egg laying number of an individual CCG/CGG at 60 weeks is obviously higher than that of other haplotype combinations, and the selection of the haplotype combination is beneficial to improving the whole later egg laying number of the laying hen, namely, the invention provides a new molecular breeding marker for marker-assisted breeding of the later egg laying character of the laying hen, realizes the screening of the later egg laying character of the laying hen, and the screening method is simple and quick.
Drawings
FIG. 1 is a schematic diagram of a technical route of the present invention;
FIG. 2 shows the result of agarose gel detection of PCR amplification products of the NCS1 gene fragment of laying hen according to the embodiment of the present invention, wherein M is Marker, and 1-5 are the results of amplification of the NCS1 gene fragment;
FIG. 3 is a comparison chart of sequencing results of individuals with different genotypes at 3 SNP polymorphic sites of the layer chicken NCS1 gene in example 1 of the present invention: (a) the 171bp has C/T mutation site; (b) the 482bp has a C/G mutation site; (c) the 567bp has an A/G mutation site;
FIG. 4 shows the result of block analysis of the haplotype of the NCS1 gene in example 4 of the present invention.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1 acquisition of partial coding region fragment of egg chicken NCS1 Gene and establishment of SNP detection method
1. Extraction of laying hen genome DNA
The test laying hen varieties comprise Jianghan chicken and Luo island red chicken, and samples are from poultry test fields of animal husbandry and veterinary research institute of agricultural and scientific institute in Hubei province. The extraction of the laying hen genome DNA adopts a genome DNA kit (operated according to the kit specification) produced by Beijing Baitacg biotechnology limited to extract, and the specific steps are as follows:
(1) about 1ml of blood was drawn from the inferior vein of the chicken wings using a disposable syringe, injected into a 1.5ml autoclaved centrifuge tube containing 200 μ L of sterile ACD anticoagulant, shaken gently, recorded wing number, and stored at-20 ℃ for future use. Sucking 10 μ L of anticoagulated blood, adding 500 μ L of BB2 and 10 μ L of proteinase K (20mg/mL), mixing well, and incubating at room temperature for 10 min;
(2) centrifuging for a short time, adding all the solution into a centrifugal column, centrifuging for 1min at 12000g, and discarding the effluent;
(3) adding 500 μ L of solution CB3, centrifuging at 12000g for 30s, and discarding the effluent;
(4) adding 500 μ L of WB3 solution, centrifuging at 12000g for 30s, and discarding the effluent;
(5) repeating the step 4 once;
(6) centrifuging at 12000g for 2min to completely remove the residual WB 3;
(7) placing the centrifugal column in a clean centrifugal tube, adding 50-200 μ L preheated EB or deionized water into the center of the column, standing at room temperature for 1min, centrifuging at 12000g for 1min, eluting DNA, and storing the eluted DNA at-20 deg.C for later use.
2. Obtaining partial nucleotide fragment of laying hen NCS1 gene
(1) PCR amplification
A primer pair is designed according to the sequence of the laying hen NCS1 Gene (accession number Gene ID: NC-006104.5) published by NCBI database, and the sequence of the primer pair is as follows:
an upstream primer: 5'-AGCAGTGGTGAGTAGCAG-3' (shown in SEQ ID NO: 2),
a downstream primer: 5'-GACATTGAACACGAAGGT-3' (shown in SEQ ID NO: 3).
The primers are used for carrying out PCR amplification in the genome DNA of the laying hens, the PCR reaction system is shown in table 1, and the PCR reaction conditions are shown in table 2.
TABLE 1 PCR reaction System
TABLE 2 PCR reaction conditions
And (3) carrying out agarose gel electrophoresis detection on the PCR amplification product, wherein the detection result is shown in figure 2, and the size of the fragment of the product is 1578 bp.
(2) Purification of PCR amplification products
The PCR amplification product is purified by using a Gel Extraction Kit of Shanghai Biotechnology engineering Co., Ltd, and the specific steps are as follows: cutting off gel containing target fragment from agarose gel, placing into 1.5mL centrifuge tube, adding 400 μ L sol solution, heating in 50-60 deg.C water bath until the gel is completely melted, mixing uniformly every 2min while heating, and cooling to room temperature; placing the centrifugal column into a collecting tube, transferring the mixed solution to the centrifugal column, and standing at room temperature for 2 min; centrifuging at 12000r/min for 1min, and adsorbing the DNA onto the column; pouring waste liquid in the collecting pipe, putting the centrifugal column into the same collecting pipe, adding 700 mu L of eluent, and centrifuging for 1min at 12000 r/min; pouring the waste liquid in the collecting pipe, and centrifuging for 1min at 12000 r/min; placing the column into a sterilized 1.5mL centrifuge tube, adding 40 μ L eluent or double distilled water (pH > 7.0), and standing at room temperature or 37 deg.C for 2-3 min; centrifuging at 12000r/min for 1min, wherein the liquid in the centrifuge tube is the recovered DNA fragment.
3. Detection of molecular markers by direct sequencing
And directly sending the PCR purified product obtained after purification to Beijing Okkomy Splending Biotechnology Limited to perform sequencing, and judging the genotype of the site in the detection group according to the sequencing result. Blast alignment analysis was performed using DNAStar software, and the analysis results are shown in fig. 3. The result shows that three allelic mutations of C/T, C/G and A/G exist at 171bp, 482bp and 567bp in the sequence respectively, the mutations cause the polymorphism of NCS1 gene, and the nucleotide sequences of the 3 SNP markers are shown in the sequence table SEQ ID NO: 1. The 3 polymorphic sites form 6 haplotypes, namely CCG, CCA, CGG, CGA, TGG and TGA.
Example 2 detection of polymorphism distribution of molecular markers of the invention in egg-laying hens
The polymorphism of 3 sites in the sequence shown in SEQ ID NO. 1 was detected, three genotypes were detected at the g.171bp C > T (NCS1_ SNP1), g.482bp C > G (NCS1_ SNP2) and g.567bp A > G (NCS1_ SNP3) sites, and the genotype frequency, allele frequency and distribution were shown in Table 3.
TABLE 3 layer chicken NCS1 genotype frequencies and allele frequencies
Note: genotype frequency the number in parentheses is the number of individuals of the genotype.
As can be seen from Table 3, 3 genotypes were detected at all 3 mutation sites of the sequence shown in SEQ ID NO. 1, and at positions NCS1_ SNP1 to NCS1_ SNP3, the dominant alleles were C, C, G, and the dominant genotypes were CC, and GG, respectively.
Example 3 correlation analysis and application of molecular marker and egg laying character of laying hens in later period
In order to determine whether the detected marks of the NCS1_ SNP 1-NCS 1_ SNP3 of the laying hens are related to the difference of the later laying character of the laying hens, Jianghan chicken and Luo island red chicken (the total number of samples is 220, wherein the Jianghan chicken and the Luo island red chicken are 110 respectively) are selected as test materials, the samples are collected in a poultry farm of animal husbandry and veterinary research institute of agricultural and scientific schools in Hubei province, the characters of the day of laying, the number of eggs laid at 40 weeks and the number of eggs laid at 60 weeks of each individual are recorded, polymorphism detection is carried out by using a direct sequencing method, and the correlation between different genotypes of partial coding regions of the NCS1 gene of the laying hens and the later laying character of the. The association analysis between genotype and phenotype was performed using SPSS18.0 software, using the following model:
Yij＝u+Gi+Pj+eij
wherein YIj is a character observed value; u is the total average value of the characters; gi is the genotype effect; pj is the fixation effect; eij is the random error.
Correlation analysis between different genotypes of 3 mutation sites and later laying traits of laying hens is carried out in Jianghhan chickens and Luo island red chickens, and the statistical analysis result is shown in table 4.
TABLE 4 Association analysis of 3 mutation site polymorphisms of layer chicken NCS1 gene and later laying traits
Note: in the above table, the same letter indicates that the difference is not significant, the letters a, b and c indicate that the difference is significant, and n is the number of individuals of the genotype.
From the analysis results in table 4, it can be seen that the NCS1_ SNP1 to NCS1_ SNP3 site polymorphisms of the NCS1 gene have significant correlations with the day-to-date-of-birth age, the egg laying number at 40 weeks of age, and the egg laying number at 60 weeks of age (p < 0.05). Wherein the TT genotype at NCS1_ SNP1 site, the CC and CG genotype at NCS1_ SNP2 site, and the AA and GG genotype at NCS1_ SNP3 site have higher egg laying number of 40 weeks old; the CT genotype at the NCS1_ SNP1 site, the CG genotype at the NCS1_ SNP2 site and the GG genotype at the NCS1_ SNP3 site have higher egg laying number of 60 weeks old.
Example 4 correlation analysis and application of molecular marker and egg laying character of laying hens in later period
1. Construction of haplotypes and haplotype combinations
Haplotype analysis of NCS1_ SNP1 to NCS1_ SNP3 is carried out by utilizing Haploview software, the obtained genotype data of the NCS1_ SNP1 to NCS1_ SNP3 sites of all individuals are input into a PHASE program, the genotype of each individual is calculated, and meanwhile, the degree of pairwise linkage disequilibrium between the sites is calculated. The haplotype block analysis results are shown in FIG. 4.
As can be seen from FIG. 4, a total of 1 haplotype block was found by analyzing the linkage disequilibrium between the loci NCS1_ SNP1 and NCS1_ SNP3, and the haplotype block was analyzed to find 6 haplotypes in the laying hen population studied in the present invention, wherein two haplotypes exist per individual, and the sequence and number statistics of each haplotype are shown in Table 5.
TABLE 5 statistic results of SNP site haplotype of NCS1 gene
| Haplotype | Sequence of | Quantity (only) | |
| H1 | CCG | 260 | |
| H2 | CCA | 72 | |
| H3 | CGG | 35 | |
| H4 | CGA | 27 | |
| H5 | TGG | 41 | |
| | TGA | 5 |
Haplotype combinations of the above haplotype compositions were analyzed for each individual, and a total of 11 haplotype combinations were found, as shown in Table 6.
TABLE 6 haplotype combinations of the NCS1 gene
| Haplotype combinations | Sequence of | Quantity (only) |
| H1H1 | CCG/CCG | 81 |
| H1H2 | CCG/CCA | 46 |
| H1H3 | CCG/CGG | 20 |
| H1H4 | CCG/ |
5 |
| H1H5 | CCG/TGG | 25 |
| H2H2 | CCA/ |
10 |
| H2H5 | CCA/TGG | 6 |
| H3H3 | CGG/ |
5 |
| H3H5 | CGG/TGG | 6 |
| H4H4 | CGA/CGA | 11 |
| H5H6 | TGG/ |
5 |
And rejecting part of haplotype combinations with small quantity in the sample population, and selecting 5 haplotype combinations with the largest quantity for association analysis.
2. Association analysis of haplotype combination and later-period egg-laying traits
The SPSS18.0 software was used to perform association analysis of haplotype combinations and late egg laying traits, and the results are shown in Table 7.
TABLE 7 correlation analysis results of advantageous haplotype combinations and later egg laying traits
Note: in the above table, the same letter indicates that the difference is not significant, the letters a, b and c indicate that the difference is significant, and n is the number of individuals of the genotype.
From the analysis results in Table 7, it was found that haplotype combination H1H 2: CCG/CCA and haplotype combination H1H 5: the 40-week-old egg production of CCG/TGG individuals was significantly higher than that of other haplotype-combined individuals (p <0.05), haplotype combination H1H 3: the 60 week old egg production of CCG/CGG individuals was significantly higher than that of other haplotype group individuals (p < 0.05). Combining the above analysis results, haplotype combination H1H 3: CCG/CGG has the best post-laying egg character. Therefore, in the laying hen population, by combining H1H 3: the selection and the retention of the CCG/CGG individuals are beneficial to improving the overall later laying character of the laying hens. Namely, the haplotype combination composed of the mutation sites identified by the invention can be used as a potential genetic marker for improving the later laying character of the laying hens so as to be used for the auxiliary selection of the high-yield laying hens.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention.
Sequence listing
<110> institute of zootechnics of academy of agricultural sciences of Hubei province
<120> molecular marker related to later-stage egg laying traits in laying hen NCS1 gene and application thereof
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1578
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<220>
<221> allele
<222> (171)..(171)
<223> y = c or t
<220>
<221> allele
<222> (482)..(482)
<223> s = c or g
<220>
<221> allele
<222> (567)..(567)
<223> r = a or g
<400> 1
agcagtggtg agtagcagcg tggcggtgag atgctggggg tgctggggga cccctcctgc 60
ttggagctca gtgcgggggc aggcagacct tggggtgcat gcagctcgga ggggaggaca 120
gcagcacacg gcagtccttt ccccgcaccc cgcatgcact ccgctccctc ycgtttgtgg 180
ctctgtgcag cggtggctct ctgcctttcc tcctgctcag cccccatggg gacgccgggg 240
ctctccgaac ggtgccacgg ggtgtacccc aatgcagccc tgaggggggc agagtctgag 300
gggcgtggac accaggacgg ggcatggagg cagggccgcc ttgcagcagc gtgctctcac 360
cccatcccct ccagctgcca aacgtgccgc cctaaaaaag ccaccgtttc cctttcatct 420
ccctatcaaa ccagtcaagg ttttctttct ttatcgcgtt ctctcatcct tctccccccc 480
tscccgatga ctcatagctg cagcagcacg aagatgtcac cggcacttca ctttcccacc 540
tcgccacccc cttcccctcc tcccccrggc cctcggcccg ctgacagatc tgggtgaggg 600
gttgtgtaat gctgctgcac acagcggggt cccagcgagg tccacgcgct gtcctcagac 660
tcacggagct gcctgacccc ctccctgcgc cctgtcagca ttggaggtgt tgcacctcct 720
tgtggctgtg ctctgtggtt ctgcctttgg ctgtgctgtc ccaggaggtg tagaagcccc 780
gctgtgcccc catttcccac gaggagtttt cacacacaca aaagcccctg cgctgcctca 840
gcttacctgg ctgcaaagct gccatcccag agcactgcac tgtggtgcct ggggctgtcg 900
tgtgattact ttttgctacg gcgtggctgc agcacgtgag tgtggaacag caggctgtgc 960
tggttggggg ctgctgtgct gggctctcat tgctgtcccc atcaaaatcc tcattctcat 1020
ccccatctcc gtctcattct catcctcgtc tccatcctca acctcatcct catccccagt 1080
atcatcccca tcccctttcc catccttttg cccatctcta tcccatcccc gtcctcattc 1140
ccatttccat cccactccca tctccgtccc catcctcatc cccgtcctca accgcttcgt 1200
catcctcatc ctcatctcca tcaccattcc catccccttt cccatcttca tgcccatccc 1260
catcctatcc ccatccgacc cctgtcccca tccccgtccc ctcatggcca atgctcaaca 1320
tggatgtggc catggagtag ttccccattc tgggcaggtg ctgtggttgg ggaccactcg 1380
atgtcaccgg tgccacttgg tgccctctgg gtcctaatat tgaaggaacc ctaaccctaa 1440
cgctccctcc ccaggtacaa gggctttatc aaggactgcc ccagtgggca gctcgatgct 1500
gccggcttcc agaagatcta caagcagttc ttcccctttg gtgacccaac caaatttgcc 1560
accttcgtgt tcaatgtc 1578
<210> 2
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 2
agcagtggtg agtagcag 18
<210> 3
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 3
gacattgaac acgaaggt 18
Claims (10)
1. The molecular marker related to later-stage egg laying traits in the laying hen NCS1 gene is characterized in that the nucleotide sequence of the molecular marker is shown in a sequence table SEQ ID NO. 1, and the molecular marker comprises:
SNP1 site: C/T polymorphic sites exist at the 171bp position in the sequence shown in SEQ ID NO. 1;
SNP2 site: the C/G polymorphic site exists at the 482bp position in the sequence shown in SEQ ID NO. 1;
SNP3 site: the A/G polymorphic site exists at the 567bp position in the sequence shown in SEQ ID NO. 1.
2. The molecular marker of claim 1, wherein the dominant alleles at 171bp, 482bp and 567bp in the sequence are C, C, G respectively, and the dominant genotypes are CC, CC and GG respectively.
3. A primer pair for amplifying the molecular marker of claim 1, wherein the primer pair comprises: the nucleotide sequence of the upstream primer is shown as SEQ ID NO. 2, and the nucleotide sequence of the downstream primer is shown as SEQ ID NO. 3.
4. A method for detecting a molecular marker related to later-stage egg laying traits in an NCS1 gene of a laying hen, which is characterized by detecting by using the primer pair of claim 3.
5. The detection method according to claim 4, characterized in that it comprises:
extracting genome DNA from blood of laying hens, amplifying by using the primer pair of claim 3 by using the genome DNA as a template, and comparing the sequence of the amplified products to obtain 3 SNP sites as described in claim 1.
6. A haplotype combination comprising SNP sites in claim 1, wherein said haplotype combination is a combination comprising SNP1-SNP3 sites.
7. A method for constructing a haplotype combination, the method comprising: firstly, detecting 3 SNP sites by using the method of claim 5; six haplotypes were then constructed, H1: CCG, H2: CCA, H3: CGG, H4: CGA, H5: TGG, H6: TGA; and then, establishing a haplotype combination according to the haplotype, and correlating the haplotype combination with the later laying traits of the laying hens.
8. Use of the molecular marker of any one of claims 1-2, or the primer pair of claim 3, or the haplotype combination of claim 6 in assisted selection of laying hens for later laying traits.
9. The use of claim 8, wherein the late stage egg laying traits of the laying hens comprise 40-week-old egg laying numbers and/or 60-week-old egg laying numbers.
10. A breeding method of late-stage high-yield laying hens is characterized by comprising the following steps: the method of claim 7, wherein the haplotype combination is constructed, and the remaining haplotype combination is HIH 3: individuals of CCG/CGG.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110285978.7A CN112852976B (en) | 2021-03-17 | 2021-03-17 | Molecular marker related to later egg laying characteristics in NCS1 gene of laying hen and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110285978.7A CN112852976B (en) | 2021-03-17 | 2021-03-17 | Molecular marker related to later egg laying characteristics in NCS1 gene of laying hen and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112852976A true CN112852976A (en) | 2021-05-28 |
| CN112852976B CN112852976B (en) | 2023-10-31 |
Family
ID=75995161
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110285978.7A Active CN112852976B (en) | 2021-03-17 | 2021-03-17 | Molecular marker related to later egg laying characteristics in NCS1 gene of laying hen and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112852976B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114134238A (en) * | 2022-01-10 | 2022-03-04 | 华南农业大学 | RALGAPA1 gene SNP molecular marker related to egg laying traits of Muscovy ducks and application thereof |
| CN114457171A (en) * | 2022-03-18 | 2022-05-10 | 江苏省家禽科学研究所 | Haplotype molecular marker related to reproductive performance of laying ducks and application thereof |
| CN116103413A (en) * | 2023-03-17 | 2023-05-12 | 湖北省农业科学院畜牧兽医研究所 | SNP (Single nucleotide polymorphism) marker related to laying characteristics of local chickens as well as detection method and application thereof |
| CN116751868A (en) * | 2023-03-17 | 2023-09-15 | 湖北省农业科学院畜牧兽医研究所 | SNP (Single nucleotide polymorphism) marker related to laying characteristics of local chickens as well as detection method and application thereof |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1287171A (en) * | 1999-09-07 | 2001-03-14 | 复旦大学 | Human neuron calcium sensing protein and its code sequence, preparation and use |
| EP1250931A1 (en) * | 2001-04-17 | 2002-10-23 | F. Hoffmann-La Roche Ag | Novel use of neuronal calcium sensor-1 (NCS-1) in therapy of CNS disorders and in the development of therapeutic agents |
| WO2007031727A2 (en) * | 2005-09-13 | 2007-03-22 | Oxford Biomedica (Uk) Limited | System for delivering neuronal calcium sensor - 1 (ncs - 1) |
| CN103635187A (en) * | 2011-04-27 | 2014-03-12 | 耶鲁大学 | Drug therapy to inhibit chemotherapy-induced adverse effects and related pharmaceutical compositions, diagnostics, screening techniques and kits |
| CN105821079A (en) * | 2010-04-23 | 2016-08-03 | 佛罗里达大学研究基金公司 | RAAV-guanylate cyclase compositions and methods for treating leber's congenital amaurosis-1 (LCA1) |
| CN107475412A (en) * | 2017-09-20 | 2017-12-15 | 山东农业大学 | A kind of molecular labeling related to chicken egg-laying deseription and its application in chicken breeding |
| EP3432911A1 (en) * | 2016-03-23 | 2019-01-30 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Targeting the neuronal calcium sensor 1 for treating wolfram syndrome |
| CN110452990A (en) * | 2018-05-07 | 2019-11-15 | 华中农业大学 | For selecting hen to lay eggs the SNP marker and its application of later period laying rate |
| CN111286543A (en) * | 2020-03-08 | 2020-06-16 | 湖北省农业科学院畜牧兽医研究所 | Haplotype marker related to egg shell quality traits in KRT14 gene and application thereof |
| CN111440878A (en) * | 2020-03-08 | 2020-07-24 | 湖北省农业科学院畜牧兽医研究所 | Haplotype marker related to quality traits of egg shells in CDH17 genes and application |
-
2021
- 2021-03-17 CN CN202110285978.7A patent/CN112852976B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1287171A (en) * | 1999-09-07 | 2001-03-14 | 复旦大学 | Human neuron calcium sensing protein and its code sequence, preparation and use |
| EP1250931A1 (en) * | 2001-04-17 | 2002-10-23 | F. Hoffmann-La Roche Ag | Novel use of neuronal calcium sensor-1 (NCS-1) in therapy of CNS disorders and in the development of therapeutic agents |
| WO2007031727A2 (en) * | 2005-09-13 | 2007-03-22 | Oxford Biomedica (Uk) Limited | System for delivering neuronal calcium sensor - 1 (ncs - 1) |
| CN105821079A (en) * | 2010-04-23 | 2016-08-03 | 佛罗里达大学研究基金公司 | RAAV-guanylate cyclase compositions and methods for treating leber's congenital amaurosis-1 (LCA1) |
| CN103635187A (en) * | 2011-04-27 | 2014-03-12 | 耶鲁大学 | Drug therapy to inhibit chemotherapy-induced adverse effects and related pharmaceutical compositions, diagnostics, screening techniques and kits |
| EP3432911A1 (en) * | 2016-03-23 | 2019-01-30 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Targeting the neuronal calcium sensor 1 for treating wolfram syndrome |
| CN107475412A (en) * | 2017-09-20 | 2017-12-15 | 山东农业大学 | A kind of molecular labeling related to chicken egg-laying deseription and its application in chicken breeding |
| CN110452990A (en) * | 2018-05-07 | 2019-11-15 | 华中农业大学 | For selecting hen to lay eggs the SNP marker and its application of later period laying rate |
| CN111286543A (en) * | 2020-03-08 | 2020-06-16 | 湖北省农业科学院畜牧兽医研究所 | Haplotype marker related to egg shell quality traits in KRT14 gene and application thereof |
| CN111440878A (en) * | 2020-03-08 | 2020-07-24 | 湖北省农业科学院畜牧兽医研究所 | Haplotype marker related to quality traits of egg shells in CDH17 genes and application |
Non-Patent Citations (5)
| Title |
|---|
| RANDOLPH B CALDWELL等: "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis", 《GENOME BIOLOGY》 * |
| 佚名: "bGalGal1.mat.broiler.GRCg7b", 《NCBI》 * |
| 佚名: "NC_006104.5", 《NCBI》 * |
| 李华龙等: "蛋鸡设施养殖环境质量评价预测模型构建方法及性能测试", 《智慧农业(中英文)》 * |
| 潘爱銮等: "不同鸡品种肉质性状比较分析", 《湖北农业科学》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114134238A (en) * | 2022-01-10 | 2022-03-04 | 华南农业大学 | RALGAPA1 gene SNP molecular marker related to egg laying traits of Muscovy ducks and application thereof |
| CN114134238B (en) * | 2022-01-10 | 2022-07-12 | 华南农业大学 | RALGAPA1 gene SNP molecular marker related to egg laying traits of Muscovy ducks and application thereof |
| CN114457171A (en) * | 2022-03-18 | 2022-05-10 | 江苏省家禽科学研究所 | Haplotype molecular marker related to reproductive performance of laying ducks and application thereof |
| CN114457171B (en) * | 2022-03-18 | 2023-09-19 | 江苏省家禽科学研究所 | Haplotype molecular marker related to reproductive performance of laying ducks and application thereof |
| CN116103413A (en) * | 2023-03-17 | 2023-05-12 | 湖北省农业科学院畜牧兽医研究所 | SNP (Single nucleotide polymorphism) marker related to laying characteristics of local chickens as well as detection method and application thereof |
| CN116751868A (en) * | 2023-03-17 | 2023-09-15 | 湖北省农业科学院畜牧兽医研究所 | SNP (Single nucleotide polymorphism) marker related to laying characteristics of local chickens as well as detection method and application thereof |
| CN116103413B (en) * | 2023-03-17 | 2023-09-22 | 湖北省农业科学院畜牧兽医研究所 | SNP (Single nucleotide polymorphism) marker related to laying characteristics of local chickens as well as detection method and application thereof |
| CN116751868B (en) * | 2023-03-17 | 2024-01-30 | 湖北省农业科学院畜牧兽医研究所 | SNP (Single nucleotide polymorphism) marker related to laying characteristics of local chickens as well as detection method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112852976B (en) | 2023-10-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112852976B (en) | Molecular marker related to later egg laying characteristics in NCS1 gene of laying hen and application thereof | |
| CN109628613B (en) | Molecular marker related to egg laying and egg quality traits of laying ducks and application thereof | |
| CN111286543B (en) | Haplotype marker related to egg shell quality traits in KRT14 gene and application thereof | |
| CN111440878B (en) | Haplotype marker related to quality traits of egg shells in CDH17 genes and application | |
| CN110592251B (en) | Main effect QTL locus of flowering phase character of brassica napus, SNP molecular marker development and application | |
| CN109112217B (en) | Genetic marker obviously related to pig body length and nipple number and application | |
| CN110205388B (en) | Molecular marking method for two mutation sites in chicken MMP-11 gene 5' regulatory region and application of molecular marking method in chicken sexual precocity trait breeding | |
| CN104017808B (en) | A kind of genetic marker relevant to goat growth trait and application | |
| CN112501346A (en) | SNP molecular marker related to rice bacterial leaf blight resistance, detection primer pair and application | |
| CN111676295A (en) | Research method of gene related to feed intake regulation | |
| CN113862375A (en) | SNP (Single nucleotide polymorphism) marker of AANAT (ananas site-directed translation) and ASMT (antisense-fluorescent marker) genes of cattle and application thereof | |
| CN110527739B (en) | Major QTL (quantitative trait locus) site of glucosinolate content of brassica napus seeds, SNP (Single nucleotide polymorphism) molecular marker and application thereof | |
| CN112176082B (en) | SNP molecular marker of wheat grain weight related gene and application thereof | |
| CN114350818B (en) | Prolactin gene SNP molecular marker related to egg laying traits of Muscovy ducks and application thereof | |
| Liu et al. | Combined effects of three novel SNPs within goat LHX3 gene on milk performance | |
| CN103525822B (en) | The molecular mark detection method that a kind of piglet diarrhea resistance is relevant and application | |
| CN110938705B (en) | Molecular marker influencing early weight of goat and primer and application thereof | |
| CN111647663B (en) | Molecular genetic marker for chicken walking number character and application | |
| CN112852977A (en) | Molecular marker related to later-stage egg laying traits in laying hen DPT gene and application thereof | |
| CN111394473B (en) | Molecular marker related to chicken antler crowns and typing method and application thereof | |
| CN108251552B (en) | Phosphoglycoside-oleate mutase gene fragment for increasing rice yield and application thereof | |
| CN101875977B (en) | Method for detecting mononucleotide polymorphism of scalper SREBP1c gene | |
| CN107937409A (en) | The clone of rice tillering angle gene TAC3 and application | |
| Xiong et al. | Two tagSNPs in the prolactin receptor gene are associated with growth and litter traits in Boer and Macheng Black crossbred goats | |
| CN108517362B (en) | Quail chestnut color feather character related molecular marker and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |