CN112840952A - Cultivation method of phallus impudicus strain - Google Patents
Cultivation method of phallus impudicus strain Download PDFInfo
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- CN112840952A CN112840952A CN202110296341.8A CN202110296341A CN112840952A CN 112840952 A CN112840952 A CN 112840952A CN 202110296341 A CN202110296341 A CN 202110296341A CN 112840952 A CN112840952 A CN 112840952A
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- 238000012364 cultivation method Methods 0.000 title claims abstract description 9
- 241001313695 Phallus impudicus Species 0.000 title abstract description 22
- 239000001963 growth medium Substances 0.000 claims abstract description 54
- 238000012258 culturing Methods 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 9
- 241000238631 Hexapoda Species 0.000 claims abstract description 7
- 241000607479 Yersinia pestis Species 0.000 claims abstract description 7
- 201000010099 disease Diseases 0.000 claims abstract description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 7
- 238000000746 purification Methods 0.000 claims abstract description 7
- 238000011218 seed culture Methods 0.000 claims abstract description 4
- 238000000926 separation method Methods 0.000 claims abstract description 4
- 230000001954 sterilising effect Effects 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 238000012360 testing method Methods 0.000 claims description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- 238000005520 cutting process Methods 0.000 claims description 9
- 244000251987 Coprinus macrorhizus Species 0.000 claims description 7
- 235000001673 Coprinus macrorhizus Nutrition 0.000 claims description 7
- KFSLWBXXFJQRDL-UHFFFAOYSA-N Peracetic acid Chemical compound CC(=O)OO KFSLWBXXFJQRDL-UHFFFAOYSA-N 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 5
- 229920001817 Agar Polymers 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 claims description 3
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 238000007664 blowing Methods 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 239000000645 desinfectant Substances 0.000 claims description 3
- 235000013312 flour Nutrition 0.000 claims description 3
- 238000003958 fumigation Methods 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 229910052602 gypsum Inorganic materials 0.000 claims description 3
- 239000010440 gypsum Substances 0.000 claims description 3
- 230000001678 irradiating effect Effects 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 239000008107 starch Substances 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 2
- 241001495452 Podophyllum Species 0.000 claims 2
- YJGVMLPVUAXIQN-XVVDYKMHSA-N podophyllotoxin Chemical compound COC1=C(OC)C(OC)=CC([C@@H]2C3=CC=4OCOC=4C=C3[C@H](O)[C@@H]3[C@@H]2C(OC3)=O)=C1 YJGVMLPVUAXIQN-XVVDYKMHSA-N 0.000 claims 2
- 241001313734 Dictyophora Species 0.000 claims 1
- 235000019353 potassium silicate Nutrition 0.000 claims 1
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 claims 1
- 239000002352 surface water Substances 0.000 claims 1
- 241000233866 Fungi Species 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 230000001737 promoting effect Effects 0.000 abstract description 3
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 2
- 239000003899 bactericide agent Substances 0.000 abstract description 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 2
- 238000011161 development Methods 0.000 abstract description 2
- 230000003716 rejuvenation Effects 0.000 abstract description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 3
- 235000017491 Bambusa tulda Nutrition 0.000 description 3
- 241001330002 Bambuseae Species 0.000 description 3
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 3
- 239000011425 bamboo Substances 0.000 description 3
- 241001516694 Cratoxylum Species 0.000 description 2
- 241001313708 Dictyophora phalloidea Species 0.000 description 2
- 239000002250 absorbent Substances 0.000 description 2
- 230000002745 absorbent Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 241000295597 Phallaceae Species 0.000 description 1
- 241000745988 Phyllostachys Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000010415 tropism Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a cultivation method of a phallus impudicus strain, and relates to the technical field of strain planting. The method for culturing the phallus impudicus strain comprises the following steps: (1) collecting seed sources: collecting fresh, disease and insect pest free and well-grown wild or family spherical late buds; (2) culturing pure mother seeds (also called first-class seeds): preparing a mother seed culture medium, and performing purification culture by a tissue block separation method to obtain a pure mother seed; (3) and (3) secondary strain culture: preparing a secondary strain culture medium, and inoculating the pure mother strain into the secondary strain culture medium; (4) and (5) culturing a third-level strain. The method is beneficial to protecting the wild resources of the phallus impudicus, developing the purification and rejuvenation work of the phallus impudicus strains, eliminating the use of chemical reagents, bactericides and the like, reducing the harmful components of the phallus impudicus strains, improving the quality of the phallus impudicus products, providing quality guarantee for large-area planting of the phallus impudicus, continuously and stably promoting the development of the edible fungus industry, simplifying the production process, facilitating the operation and being beneficial to popularization.
Description
Technical Field
The invention relates to the technical field of strain planting, in particular to a cultivation method of a phallus impudicus strain.
Background
Phantom album L.pets, belonging to family Phallaceae, genus Phallus, fungus of genus Phallus, alias Phallus, fungus of genus Phyllostachys, fungus. Li Taihui et al (2004) studied in Guillain Yunnan and Guillain, and demonstrated that Binkao is distributed in Shanxi, inner Mongolia, Liaoning, Jilin, Heilongjiang, Jiangsu, Anhui, Shandong, Henan, Hubei, Guangdong, Hainan, Sichuan, Yunnan, Tibet, Gansu and Asia (Asian temperate zone such as Japan and Korea), Europe, Africa, North America, south America, and is originated in bamboo forest, forest or grassland; through investigation, Guizhou also has a large amount of wild Coprinus cinereus resources distributed, and the Coprinus cinereus grows in groups in summer and autumn or grows singly in leaf layer on the ground in a forest. It is recorded in the national Chinese herbal medicine assembly (2014) that it has important food and medicinal value, sweet and light taste, warm nature and heart meridian tropism. Has effects of promoting blood circulation, removing dampness, and relieving pain, and can be used for treating rheumatalgia.
The high-yield cultivation method of the phallus impudicus, which is invented by the Chinese patent and is in the patent number CN103004475A, adopts a large amount of chemicals, so that the production cost of the phallus impudicus is too high, the environment is easily polluted, toxic components in the phallus impudicus are not reduced, the toxic components are increased, and the later purification cost is increased.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a cultivation method of a phallus impudicus strain, and solves the problems of low product quality and high cost of the phallus impudicus cultivation in the prior art.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: a cultivation method of a Coprinus cinereus strain comprises the following steps: (1) collecting seed sources: collecting fresh, disease and insect pest free and well-grown wild or family spherical late buds; (2) culturing pure mother seeds (also called first-class seeds): preparing a mother seed culture medium, and performing purification culture by a tissue block separation method to obtain a pure mother seed; (3) and (3) secondary strain culture: preparing a secondary strain culture medium, and inoculating the pure mother strain into the secondary strain culture medium; (4) and (3) three-stage strain culture: and (4) preparing a third-level strain culture medium, and inoculating the second-level strain into the third-level strain culture medium.
Preferably, the step (1) of seed source collection, (2) of pure mother strain, (3) of secondary strain, and (4) of tertiary strain culture, specifically, collecting the later stage buds of the bally cratoxylum impunitum in the wild or domestic environment, putting the later stage buds of the bally cratoxylum impunitum into a collection box (basket), selecting the fresh later stage buds of the bally wild or domestic environment with good growth without plant diseases and insect pests, cleaning the selected buds with clear water, cutting off residual rhizomes at the base part with a flame or a knife sterilized by 75% alcohol, sterilizing the surface with 75% alcohol in an inoculation box or an ultra-clean workbench, finally cleaning with distilled water, sucking the surface moisture with absorbent paper, cutting the buds with a flame or a knife sterilized by 75% alcohol in a transverse and longitudinal direction in a cross shape, taking out tissue blocks in a colloid body with tweezers, inoculating the tissue blocks into a mother strain (culture dish) culture medium, and culturing in a dark place at 22-24 ℃. After hyphae germinate and grow to 0.5-1.5 cm, cutting and inoculating thick hyphae with few branched edges of bacterial colonies to a mother strain (test tube inclined plane) culture medium, carrying out light-tight culture at 22-24 ℃ for 20-30 d to obtain a pure mother strain, inoculating the mother strain to a secondary strain (glass bottle) culture medium, inoculating 2-3 bottles of the secondary strain culture medium to each test tube, carrying out light-tight culture at 22-24 ℃ for 60-90 d to obtain a secondary strain, inoculating the secondary strain to a tertiary strain (plastic bag) culture medium, inoculating 30-40 bags of each bottle, and carrying out light-tight culture at 22-24 ℃ for 70-90 d to obtain a tertiary strain.
Preferably, the pure mother culture medium is calculated according to the weight number required by 10 culture dishes or 10 test tubes, and comprises 0.5g of starch, 0.05g of bamboo leaves, 0.5g of agar powder, 0.1g of glucose and 500g of distilled water under the natural pH condition; sterilizing at 121 ℃ under 0.105MPa for 60min, and making into a 60mm culture dish plate culture medium or a 18 x 180mm (or 18 x 220mm) test tube slant culture medium, wherein the second-level strain culture medium comprises 70 parts by weight of sawdust, 20 parts by weight of corn flour, 0.5 part by weight of gypsum powder, 0.5 part by weight of sucrose and 60 parts by weight of water; subpackaging with 500ml saline glass bottles, wherein the loading amount is 450ml, sterilizing at 100 ℃ for 10-12 h under normal pressure, or sterilizing at 121 ℃ under 0.105MPa for 2-3 h to prepare a secondary strain culture medium. The third strain culture medium is the same as the second strain culture medium, and is packaged by using a special strain culture plastic bag, and the loading amount is 1000g for cultivating the dictyophora phalloidea.
Preferably, in the step (1), the diameter of the selected spherical late buds is 7-16 cm.
Preferably, in the step (2), an outer flame for flame sterilization is used, and the sterilization time is 1 min; irradiating with ultraviolet for 15min before use, and blowing air for 15min or ventilating for 30 min; the inoculation box is fumigated with fumigation disinfectant (ethylene oxide and peracetic acid) for 30min before use.
(III) advantageous effects
The invention provides a culture method of a phallus impudicus strain. The method has the following beneficial effects:
the method is beneficial to protecting the wild resources of the phallus impudicus, developing the purification and rejuvenation work of the phallus impudicus strains, eliminating the use of chemical reagents, bactericides and the like, reducing the harmful components of the phallus impudicus strains, improving the quality of the phallus impudicus products, providing quality guarantee for large-area planting of the phallus impudicus, and continuously and stably promoting the development of the edible fungus industry, and the method is simplified in production process, convenient to operate, beneficial to popularization and promotion
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example (b):
the embodiment of the invention provides a method for culturing a phallus impudicus strain, which comprises the following steps: (1) collecting seed sources: collecting fresh, disease and insect pest free and well-grown wild or family spherical late buds; (2) culturing pure mother seeds (also called first-class seeds): preparing a mother seed culture medium, and performing purification culture by a tissue block separation method to obtain a pure mother seed; (3) and (3) secondary strain culture: preparing a secondary strain culture medium, and inoculating the pure mother strain into the secondary strain culture medium; (4) and (3) three-stage strain culture: and (4) preparing a third-level strain culture medium, and inoculating the second-level strain into the third-level strain culture medium.
Collecting balled later-stage buds of the white ghost pen in a wild or family environment, putting the balled later-stage buds into a collection box (basket), selecting the fresh balled later-stage buds of the wild or family with good growth without plant diseases and insect pests, cleaning the selected buds with clear water, cutting off residual rhizomes on the base by using flame or a knife sterilized by 75% alcohol, performing surface sterilization by using 75% alcohol in an inoculation box or an ultra-clean workbench, cleaning with distilled water, sucking dry the surface moisture by using absorbent paper, cutting the buds according to a transverse and longitudinal cross by using the flame or the knife sterilized by 75% alcohol, taking out tissue blocks in a colloidal body by using tweezers, inoculating the mycelia into a culture medium of the mother seed (culture dish), performing light-tight culture at 22 ℃, cutting the colonies with few branched edges, performing germination and growth to 0.5cm, and then performing culture, Inoculating the stout hyphae into a mother strain (test tube slant) culture medium, culturing in the dark at 22 ℃ for 20d to obtain a pure mother strain, inoculating the mother strain into a second-level strain (glass bottle) culture medium, inoculating 2 bottles of the second-level strain culture medium into each test tube, culturing in the dark at 22 ℃ for 60d to obtain a second-level strain, inoculating the second-level strain into a third-level strain (plastic bag) culture medium, inoculating 30 bags of each bottle, and culturing in the dark at 22 ℃ for 70d to obtain a third-level strain.
The pure mother culture medium is calculated according to the weight number required by 10 culture dishes or 10 test tubes, and comprises 0.5g of starch, 0.05g of bamboo leaves, 0.5g of agar powder, 0.1g of glucose and 500g of distilled water under the natural pH condition; sterilizing at 121 ℃ under 0.105MPa for 60min, and making into a 60mm culture dish plate culture medium or a 18 x 180mm (or 18 x 220mm) test tube slant culture medium, wherein the second-level strain culture medium comprises 70 parts by weight of sawdust, 20 parts by weight of corn flour, 0.5 part by weight of gypsum powder, 0.5 part by weight of sucrose and 60 parts by weight of water; subpackaging with 500ml saline glass bottle, charging 450ml, sterilizing at 100 deg.C under normal pressure for 10h, or sterilizing at 121 deg.C under 0.105MPa for 2h, and making into secondary strain culture medium. The third strain culture medium is the same as the second strain culture medium, and is packaged by using a special strain culture plastic bag, and the loading amount is 1000g for cultivating the dictyophora phalloidea.
In the step (1), the diameter of the selected spherical late buds is 7 cm. In the step (2), outer flame is used for flame disinfection, and the disinfection time is 1 min; irradiating with ultraviolet for 15min before use, and blowing air for 15min or ventilating for 30 min; the inoculation box is fumigated with fumigation disinfectant (ethylene oxide and peracetic acid) for 30min before use.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. A method for culturing a Coprinus cinereus strain is characterized by comprising the following steps: (1) collecting seed sources: collecting fresh, disease and insect pest free and well-grown wild or family spherical late buds; (2) culturing pure mother seeds (also called first-class seeds): preparing a mother seed culture medium, and performing purification culture by a tissue block separation method to obtain a pure mother seed; (3) and (3) secondary strain culture: preparing a secondary strain culture medium, and inoculating the pure mother strain into the secondary strain culture medium; (4) and (3) three-stage strain culture: and (4) preparing a third-level strain culture medium, and inoculating the second-level strain into the third-level strain culture medium.
2. A cultivation method of Podophyllum albopictus strain as claimed in claim 1, wherein the steps of (1) seed collection, (2) pure mother strain, (3) secondary strain, and (4) tertiary strain cultivation are that collecting later stage ballets of Podophyllum albopictus in wild or domestic environment, placing them into collection box (basket), selecting out fresh later stage ballets of wild or domestic ballets which are free from plant diseases and insect pests and have good growth, cleaning the selected ballets with clear water, cutting off residual rhizomorph at base with flame or 75% alcohol sterilized knife, sterilizing surface with 75% alcohol in inoculation box or super clean bench, cleaning with distilled water, sucking water to remove surface water, cutting open the ballets with flame or 75% alcohol sterilized knife in transverse and longitudinal direction, taking out tissue blocks in the colloidal matter, inoculating to the mother strain (culture dish) culture medium, culturing in dark at 22-24 ℃, cutting strong hyphae with few branched edges of bacterial colonies after the hyphae germinate and grow to 0.5-1.5 cm, inoculating the hyphae into a mother strain (test tube inclined plane) culture medium, culturing in dark at 22-24 ℃ for 20-30 d to obtain a pure mother strain, inoculating the mother strain into a secondary strain (glass bottle) culture medium, inoculating 2-3 bottles of secondary strain culture medium into each test tube, culturing in dark at 22-24 ℃ for 60-90 d to obtain a secondary strain, inoculating the secondary strain into a tertiary strain (plastic bag) culture medium, inoculating 30-40 bags of each bottle, and culturing in dark at 22-24 ℃ for 70-90 d to obtain a tertiary strain.
3. A cultivation method of Coprinus cinereus strain as claimed in claim 1, wherein the pure mother culture medium comprises starch 0.5g, folium Bambusae 0.05g, agar powder 0.5g, glucose 0.1g, distilled water 500g, and pH natural conditions, calculated according to the weight number required by 10 petri dishes or 10 test tubes; sterilizing at 121 ℃ under 0.105MPa for 60min, and making into a 60mm culture dish plate culture medium or a 18 x 180mm (or 18 x 220mm) test tube slant culture medium, wherein the second-level strain culture medium comprises 70 parts by weight of sawdust, 20 parts by weight of corn flour, 0.5 part by weight of gypsum powder, 0.5 part by weight of sucrose and 60 parts by weight of water; subpackaging with 500ml saline water glass bottles, wherein the loading amount is 450ml, sterilizing at 100 ℃ for 10-12 h under normal pressure, or sterilizing at 121 ℃ under the pressure of 0.105MPa for 2-3 h to prepare a secondary strain culture medium, wherein the third-level strain culture medium is the same as the secondary strain culture medium, subpackaging with a special strain culture plastic bag, and the loading amount is 1000g for cultivation of the winter dictyophora.
4. The method for culturing Coprinus cinereus strains according to claim 1, wherein the diameter of the picked late balled buds is 7-16 cm in step (1).
5. The method for culturing Coprinus cinereus strain according to claim 1, wherein in step (2), the flame is sterilized with outer flame for 1 min; irradiating with ultraviolet for 15min before use, and blowing air for 15min or ventilating for 30 min; the inoculation box is fumigated with fumigation disinfectant (ethylene oxide and peracetic acid) for 30min before use.
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| CN202110296341.8A CN112840952A (en) | 2021-03-19 | 2021-03-19 | Cultivation method of phallus impudicus strain |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101578945A (en) * | 2009-06-22 | 2009-11-18 | 贵州信邦中药发展有限公司 | Cultivating method of tuckahoe |
| CN103004475A (en) * | 2013-01-04 | 2013-04-03 | 贵州省现代中药材研究所 | High-yield cultivation method of phallus impudicus |
| CN105935027A (en) * | 2016-07-08 | 2016-09-14 | 贵州省农作物品种资源研究所 | Phallus impudicus strain stick soil-covered cultivation method |
-
2021
- 2021-03-19 CN CN202110296341.8A patent/CN112840952A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101578945A (en) * | 2009-06-22 | 2009-11-18 | 贵州信邦中药发展有限公司 | Cultivating method of tuckahoe |
| CN103004475A (en) * | 2013-01-04 | 2013-04-03 | 贵州省现代中药材研究所 | High-yield cultivation method of phallus impudicus |
| CN105935027A (en) * | 2016-07-08 | 2016-09-14 | 贵州省农作物品种资源研究所 | Phallus impudicus strain stick soil-covered cultivation method |
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