CN112831538A - Quick fungus detection reagent - Google Patents
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- CN112831538A CN112831538A CN202110044206.4A CN202110044206A CN112831538A CN 112831538 A CN112831538 A CN 112831538A CN 202110044206 A CN202110044206 A CN 202110044206A CN 112831538 A CN112831538 A CN 112831538A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/045—Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The invention discloses a rapid fungus detection reagent which is prepared from the following raw materials in parts by mass: 10-20 parts of maltose, 5-10 parts of peptone, 0.05-0.1 part of vitamin B, 4-5 parts of yeast extract, 10-20 parts of agar, 0.5-1 part of distilled water and 0.33-0.55 part of auxiliary materials. Has the advantages that: the fungi can be cultured by manufacturing a fungus culture medium, the whole fungi can be rapidly cultured for three hours by mainly utilizing glass slides and micro-culture, and therefore rapid detection on the fungi can be achieved.
Description
Technical Field
The invention relates to the technical field of medicine preparation, in particular to a rapid fungus detection reagent.
Background
Fungi belong to eukaryotes and widely exist in the nature, most fungi are beneficial to human beings, few infected human beings can cause serious diseases, the fungal infection is divided into superficial fungal infection and deep fungal infection according to different infected parts, most invasive fungal infection belongs to deep fungal infection, the detection is carried out by using a molecular biology method at present, most PCR detection is carried out, a certain amount of pathogenic bacteria can be detected, the common PCR detection needs a complex sample treatment process at the early stage, a complex test procedure and a complex result judgment, and the operation process is complex.
An effective solution to the problems in the related art has not been proposed yet.
Disclosure of Invention
Aiming at the problems in the related art, the invention provides a rapid fungus detection reagent to overcome the technical problems in the prior related art.
The technical scheme of the invention is realized as follows:
according to one aspect of the present invention, a rapid fungal detection reagent is provided.
The rapid fungus detection reagent is prepared from the following raw materials in parts by mass:
10-20 parts of maltose, 5-10 parts of peptone, 0.05-0.1 part of vitamin B, 4-5 parts of yeast extract, 10-20 parts of agar, 0.5-1 part of distilled water and 0.33-0.55 part of auxiliary materials.
Further, the auxiliary materials comprise the following raw material components: 0.03-0.05 part of chloramphenicol and 0.3-0.5 part of cycloheximide.
According to another aspect of the present invention, a method for preparing a rapid fungal detection reagent is provided.
The preparation method of the rapid fungus detection reagent comprises the following steps:
firstly, placing peptone and agar into a triangular flask filled with distilled water, and heating for dissolving;
then placing the maltose into another triangular flask filled with distilled water to dissolve the maltose;
mixing the dissolved solution with vitamin B, yeast extract, chloramphenicol and cycloheximide, and heating;
subpackaging the mixed solution into test tubes, plugging a cotton plug, and performing high-pressure steam sterilization;
and cooling the sterilized solution, and storing in a refrigerator.
Further, the pressure of the above-mentioned autoclaving was 68.95kPa for 15 minutes.
Further, the cooled solution was stored in a refrigerator at 4 ℃.
Further, the pH of the solution is 5.5 to 6.0.
Further, the solution is sterilized and stored on a slant.
The raw materials adopted by the invention are explained as follows:
maltose: maltose is disaccharide formed by connecting two glucose units through alpha-1, 4 glycosidic bonds, is also called maltose, the traditional maltose is made of wheat and glutinous rice, is sweet and delicious, has rich nutrition, has the effects of expelling toxin, beautifying, tonifying spleen, replenishing qi, moistening lung, relieving cough and the like, and is a food suitable for people of all ages.
Peptone: is an organic compound. Peptone is faint yellow powder which is prepared by hydrolyzing meat, casein or gelatin with acid or protease and drying, has special smell of meat flavor, can be used as main raw material of microorganism culture medium, has large dosage in the fields of antibiotics, medical industry, fermentation industry, biochemical products, microbiological research and the like, and can be used for treating digestive tract diseases.
Vitamin B: are a generic term for B vitamins, which are often derived from the same food source, such as yeast and the like. The vitamin B is sufficient, so that nerve cells are energetic, anxiety and tension can be relieved, and the tolerance to noise and the like is increased; on the contrary, it results in a decline in the ability to cope with stress and even causes neuritis.
Yeast extract: at present, the yeast extract is pasty yeast extract in China, is rich in complete protein and balanced essential amino acid, B vitamins, nucleotide, trace elements and the like, is the most ideal raw material of a biological culture medium and a main raw material in the fermentation industry, has the effect equivalent to 8 times that of yeast, and can greatly improve the production rate of strains and the yield of fermentation products.
Agar: is polysaccharide extracted from seaweed, and is one of the most widely used seaweed gums in the world at present. It features its physical and chemical properties of coagulability and stability, and can form complex with some substances, and can be used as thickening agent, coagulating agent, suspending agent, emulsifying agent, antistaling agent and stabilizing agent.
Chloramphenicol: is an antibiotic, and can inhibit bacterial growth.
Cycloheximide: an antibiotic extracted from actinomycete fermentation liquid for producing cycloheximide can inhibit the growth of pollution fungi and cryptococcus.
The invention has the beneficial effects that: the fungi can be cultured by manufacturing a fungus culture medium, the whole fungi can be rapidly cultured for three hours by mainly utilizing glass slides and micro-culture, and therefore rapid detection on the fungi can be achieved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a flow chart of a method for preparing a reagent for rapid fungal detection according to an embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments that can be derived by one of ordinary skill in the art from the embodiments given herein are intended to be within the scope of the present invention.
According to an embodiment of the present invention, a rapid fungal detection reagent is provided.
The rapid fungus detection reagent is prepared from the following raw materials in parts by mass:
10-20 parts of maltose, 5-10 parts of peptone, 0.05-0.1 part of vitamin B, 4-5 parts of yeast extract, 10-20 parts of agar, 0.5-1 part of distilled water and 0.33-0.55 part of auxiliary materials.
Wherein, the auxiliary materials comprise the following raw material components: 0.03-0.05 part of chloramphenicol and 0.3-0.5 part of cycloheximide.
In order to clearly understand the technical scheme of the invention, the technical scheme of the invention is described in detail through specific examples.
Example one
A quick fungus detection reagent is prepared from the following raw materials in parts by mass:
10g of maltose, 5g of peptone, 0.05g of vitamin B, 4g of yeast extract, 10g of agar, 0.5g of distilled water and 0.33g of auxiliary materials.
Wherein, the auxiliary materials comprise the following raw material components: chloramphenicol 0.03g and cycloheximide 0.3 g.
The preparation method of the rapid fungus detection reagent comprises the following steps:
firstly, placing 5g of peptone and 10g of agar into a triangular flask filled with 0.5g of distilled water, and heating for dissolving;
then 10g of maltose is put into another triangular flask with 0.5g of distilled water to be dissolved;
mixing and heating the dissolved solution with 0.05g of vitamin B, 4g of yeast extract, 0.03g of chloramphenicol and 0.3g of cycloheximide;
subpackaging the mixed solution into test tubes, plugging a cotton plug, and performing high-pressure steam sterilization;
and cooling the sterilized solution, and storing in a refrigerator.
Example two
A quick fungus detection reagent is prepared from the following raw materials in parts by mass:
15g of maltose, 8g of peptone, 0.08g of vitamin B, 4.5g of yeast extract, 15g of agar, 0.8g of distilled water and 0.44g of auxiliary materials.
Wherein, the auxiliary materials comprise the following raw material components: chloramphenicol 0.04g and cycloheximide 0.4 g.
The preparation method of the rapid fungus detection reagent comprises the following steps:
firstly, placing 8g of peptone and 15g of agar into a triangular flask filled with 0.8g of distilled water, and heating for dissolving;
then 15g of maltose is put into another triangular flask with 0.8g of distilled water to be dissolved;
mixing the dissolved solution with 0.08g of vitamin B, 4.5g of yeast extract, 0.04g of chloramphenicol and 0.4g of cycloheximide, and heating;
subpackaging the mixed solution into test tubes, plugging a cotton plug, and performing high-pressure steam sterilization;
and cooling the sterilized solution, and storing in a refrigerator.
EXAMPLE III
A quick fungus detection reagent is prepared from the following raw materials in parts by mass:
20g of maltose, 10g of peptone, 0.1g of vitamin B, 5g of yeast extract, 20g of agar, 1g of distilled water and 0.55g of auxiliary materials.
Wherein, the auxiliary materials comprise the following raw material components: chloramphenicol 0.05g and cycloheximide 0.5 g.
The preparation method of the rapid fungus detection reagent comprises the following steps:
firstly, placing 10g of peptone and 20g of agar into a triangular flask filled with 1g of distilled water, and heating for dissolving;
then 20g of maltose is put into another triangular flask with 1g of distilled water to be dissolved;
mixing and heating the dissolved solution with 0.1g of vitamin B, 5g of yeast extract, 0.05g of chloramphenicol and 0.5g of cycloheximide;
subpackaging the mixed solution into test tubes, plugging a cotton plug, and performing high-pressure steam sterilization;
and cooling the sterilized solution, and storing in a refrigerator.
For the convenience of understanding the above technical solution of the present invention, the following detailed description is made on the flow of the above solution of the present invention with reference to the accompanying drawings, and specifically is as follows:
according to the embodiment of the invention, the preparation method of the rapid fungus detection reagent is also provided.
As shown in FIG. 1, in the actual production process, the preparation of the rapid fungus detection reagent comprises the following steps:
step S101, firstly, placing peptone and agar into a triangular flask filled with distilled water, and heating for dissolving;
step S103, dissolving maltose in another triangular flask filled with distilled water;
step S105, mixing and heating the dissolved solution with vitamin B, yeast extract, chloramphenicol and cycloheximide;
step S107, subpackaging the mixed solution into test tubes, plugging a cotton plug, and performing high-pressure steam sterilization;
and step S109, cooling the sterilized solution, and storing the cooled solution in a refrigerator.
In one embodiment, the pressure of the autoclaving is 68.95kPa for 15 minutes.
In one embodiment, the cooled solution is stored in a refrigerator at 4 ℃.
In one embodiment, the pH of the solution is 5.5 to 6.0.
In one embodiment, the solution is stored on a slant after sterilization.
In summary, according to the technical scheme of the invention, the fungi can be cultured by preparing the fungus culture medium, and the complete fungi can be rapidly cultured for three hours by mainly utilizing glass slides and micro-culture, so that the fungi can be rapidly detected.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (7)
1. The rapid fungus detection reagent is characterized by being prepared from the following raw materials in parts by mass:
10-20 parts of maltose, 5-10 parts of peptone, 0.05-0.1 part of vitamin B, 4-5 parts of yeast extract, 10-20 parts of agar, 0.5-1 part of distilled water and 0.33-0.55 part of auxiliary materials.
2. The reagent for rapid detection of fungi according to claim 1, wherein the auxiliary materials comprise the following raw materials: 0.03-0.05 part of chloramphenicol and 0.3-0.5 part of cycloheximide.
3. A method for preparing a rapid fungal detection reagent according to claim 3, comprising the steps of:
firstly, placing peptone and agar into a triangular flask filled with distilled water, and heating for dissolving;
then placing the maltose into another triangular flask filled with distilled water to dissolve the maltose;
mixing the dissolved solution with vitamin B, yeast extract, chloramphenicol and cycloheximide, and heating;
subpackaging the mixed solution into test tubes, plugging a cotton plug, and performing high-pressure steam sterilization;
and cooling the sterilized solution, and storing in a refrigerator.
4. The method for preparing a reagent for rapid detection of fungi according to claim 3 wherein the pressure of the autoclaving is 68.95kPa for 15 minutes.
5. The method for preparing a reagent for rapid detection of fungi according to claim 3, wherein the cooled solution is stored in a refrigerator at 4 ℃.
6. The method for preparing a reagent for rapid detection of fungi according to claim 3 wherein the pH of the solution is 5.5 to 6.0.
7. The method for preparing a reagent for rapid detection of fungi according to claim 3, wherein the solution is stored on a slant after sterilization.
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|---|---|---|---|
| CN202110044206.4A CN112831538A (en) | 2021-01-13 | 2021-01-13 | Quick fungus detection reagent |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110044206.4A CN112831538A (en) | 2021-01-13 | 2021-01-13 | Quick fungus detection reagent |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1900293A (en) * | 2006-07-11 | 2007-01-24 | 中国医学科学院皮肤病研究所 | Modified skin tinea fungus test culture medium |
| EP2348127A1 (en) * | 2010-01-25 | 2011-07-27 | Albert-Ludwigs-Universität Freiburg | Method for producing a biosensor for an in vitro screening system for identifying anti-infective substances, and uses thereof |
| CN103614452A (en) * | 2013-11-13 | 2014-03-05 | 烟台市喜旺食品有限公司 | Composite culture medium and method for quickly detecting total bacterial count by using same |
| CN110607243A (en) * | 2019-10-22 | 2019-12-24 | 中秀科技股份有限公司 | Culture medium for separating fungi and yeast-like fungi and preparation method thereof |
-
2021
- 2021-01-13 CN CN202110044206.4A patent/CN112831538A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1900293A (en) * | 2006-07-11 | 2007-01-24 | 中国医学科学院皮肤病研究所 | Modified skin tinea fungus test culture medium |
| EP2348127A1 (en) * | 2010-01-25 | 2011-07-27 | Albert-Ludwigs-Universität Freiburg | Method for producing a biosensor for an in vitro screening system for identifying anti-infective substances, and uses thereof |
| CN103614452A (en) * | 2013-11-13 | 2014-03-05 | 烟台市喜旺食品有限公司 | Composite culture medium and method for quickly detecting total bacterial count by using same |
| CN110607243A (en) * | 2019-10-22 | 2019-12-24 | 中秀科技股份有限公司 | Culture medium for separating fungi and yeast-like fungi and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 朱红梅、徐红、温海: "医学真菌常用培养基的制备和应用", 《中国真菌学杂志》 * |
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