CN112827533A - Desktop type micro-droplet chemical reaction experiment platform based on super-hydrophobic material - Google Patents
Desktop type micro-droplet chemical reaction experiment platform based on super-hydrophobic material Download PDFInfo
- Publication number
- CN112827533A CN112827533A CN202110026465.4A CN202110026465A CN112827533A CN 112827533 A CN112827533 A CN 112827533A CN 202110026465 A CN202110026465 A CN 202110026465A CN 112827533 A CN112827533 A CN 112827533A
- Authority
- CN
- China
- Prior art keywords
- micro
- droplet
- platform
- droplets
- hydrophobic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/02—Laboratory benches or tables; Fittings therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/30—Micromixers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L1/00—Enclosures; Chambers
- B01L1/50—Enclosures; Chambers for storing hazardous materials in the laboratory, e.g. cupboards, waste containers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
Abstract
The invention discloses a desktop type micro-droplet chemical reaction experiment platform based on a super-hydrophobic material and an experiment method using the same. The experimental platform comprises a micro-droplet bearing part, a micro-droplet transfer part, a micro-droplet oscillation reaction part, a main body frame and a mechanical control assembly, wherein the micro-droplet transfer part realizes the transfer of micro-droplets from the micro-droplet bearing part to the micro-droplet oscillation reaction part; the micro-droplet oscillation reaction component drives the driving wheel and the driving rod to move through the motor, so that the oscillation platform and the super-hydrophobic bowl-shaped vessel on the oscillation platform vibrate in a waveform mode, and the micro-droplets after mixing periodically rotate and roll in the super-hydrophobic bowl-shaped vessel, so that the purposes of oscillation, mixing and acceleration of droplet reaction are achieved.
Description
Technical Field
The invention relates to the fields of chemistry, materials and electromechanics, in particular to a desktop type micro-droplet chemical reaction experiment platform based on a super-hydrophobic material and an experiment method using the same.
Background
Conventional chemical reactions often require the consumption of liquid chemical reagents in volumes of tens, hundreds, or even larger volumes during chemical analysis and detection. The use of a large amount of liquid chemical reagents increases the experimental cost and the waste liquid treatment cost; the cleaning of the chemical reaction vessel can cause the waste and pollution of a large amount of water resources and increase the wastewater treatment cost; the consumption of a large amount of chemical reagents causes environmental pollution and energy waste in the processes from production to transportation and use. Reducing the consumption of liquid chemical agents from the source can effectively solve these problems.
Microfluidic technology is a common way of performing microchemical reactions at present, uses microchannels (with dimensions of tens to hundreds of microns) to process or manipulate micro-fluids, has the characteristics of miniaturization and integration, and is also commonly referred to as microfluidic chips and lab-on-a-chip. By using microfluidic technology, chemical reactions (including sample introduction, mixing, reaction, separation, detection) can be integrated into a microchip. The microfluidic chip is generally manufactured on materials such as silicon wafers, glass, polydimethylsiloxane, polymethyl methacrylate and the like by photoetching, etching and machining methods, and has the defects of high price of manufacturing equipment, complex preparation process and the like, so that the manufacturing cost is high, and the microfluidic chip cannot be applied to common micro-droplet chemical reaction.
In recent years, the development of a micro-droplet reaction by controlling the wettability of the material surface has been attracting attention. The Chinese invention patent (CN105833814B) provides a liquid drop self-driven type micro-reactor, which provides a driving force for micro-liquid drops to realize the accurate control of the volume ratio of the liquid drops, and the full mixing and the rapid transportation of the liquid drops in a micro-reactor flow channel through the gradient wettability design of a hydrophilic-hydrophobic coating. However, the preparation of the micro flow channel still adopts the mask photoetching method of the traditional micro flow chip, the surface treatment process is very complicated, and the preparation cost is difficult to reduce. Chinese patent CN110523451A prepares a magnetic elastic super-hydrophobic substrate, and by applying an external magnetic field, the surface of a reactor generates elastic depressions, and the conveying, rotary stirring and mixing of micro-droplets are realized by utilizing the synergistic effect of gravity and the Laplace force of the droplets. The material and the method only carry out the operation of the liquid drop on the surface of the magnetic elastic super-hydrophobic substrate, can not realize the transfer and the extraction of the liquid drop in a three-dimensional space, have low stirring and mixing efficiency of the liquid drop, and can not be used for the actual chemical reaction of the micro-liquid drop. Chinese patent CN207745854U designs a micro-reactor based on magnetic liquid marble, which controls the magnetic liquid marble with super-hydrophobic/oil characteristics by controlling the magnetic field to induce the violent convection of the liquid inside the marble and strengthen the disturbance, so that the reactants are fully mixed. According to the method, the reaction liquid drops and the magnetic particle powder are mixed, and the mixed magnetic particles can interfere the chemical reaction of the micro-liquid drops and influence the reaction result. Yang et al (Advanced materials, 2018, 30(9), 1704912) adopt 3D printing technology to prepare a droplet microreactor with a high-adhesion whisk microstructure, and carry out a micro-droplet reaction experiment of ferric trichloride and sodium hydroxide, but the microreactor has a complex manufacturing process and high cost, cannot be subjected to oscillation operation in the reaction process, and has a single function. So far, a low-cost method and a low-cost device capable of realizing carrying, transferring or transferring and mixing reaction of micro-droplets are not reported, which is also a technical difficulty for realizing chemical reaction of micro-droplets at present.
Disclosure of Invention
Aiming at the defects existing in the background, the invention aims to provide a desktop type micro-droplet chemical reaction experiment platform based on a super-hydrophobic material, which can realize a series of micro-chemical reaction operations such as carrying, lossless transfer, oscillation mixing and the like of micro-droplets and has the characteristics of simple operation, multiple functions, low cost, flexibility, high efficiency and function expansion.
In order to achieve the purpose, the invention is realized by the following technical scheme:
a micro-droplet chemical reaction experiment platform based on a super-hydrophobic material comprises a micro-droplet bearing part, a micro-droplet transfer part, a micro-droplet oscillation reaction part, a main body frame and a mechanical control assembly,
the main body frame comprises a cavity and an operating platform arranged in the cavity;
the micro-droplet bearing part is fixed on the operating platform and is provided with one or more bearing pieces, and the bearing pieces are provided with drawable drainage cavities;
the micro-droplet oscillation reaction component is characterized in that an oscillation platform of the micro-droplet oscillation reaction component is arranged on the operation platform, and a hydrophobic cavity is arranged on the oscillation platform; the oscillating platform passes through a hole at the corresponding position of the operating platform through a driving rod rotatably connected with the lower surface of the oscillating platform and is connected with a driving component at the lower part of the oscillating platform, and the curved oscillation of the oscillating platform is realized through the lower driving component;
four linear optical axis guide rods of the mechanical control assembly are respectively arranged on the side surface of the main body frame to form an outer peripheral guide rod; the two linear optical axis guide rods are crossed into a cross shape and are arranged in the cavity of the main body frame to form inner cavity guide rods, the outer end parts of the inner cavity guide rods are connected with the outer periphery guide rods in a sliding mode through first connecting pieces, and the two inner cavity guide rods are connected with each other through second connecting pieces; a vertical micro guide rail is arranged on the second connecting piece;
the micro-droplet transfer component is connected with the micro guide rail in a sliding manner, so that the micro-droplet is transferred from the micro-droplet bearing component to the micro-droplet oscillation reaction component.
Optionally, the receiving part comprises a drawing tray fixedly connected with the edge of the operating platform notch, a slide way is formed between the drawing tray and the lower part of the outer edge of the operating platform notch, the drawing plate slides on the slide way through the outer edge parts on two sides of the drawing plate, and the drainage cavity is placed on the drawing plate. The hydrophobic cavity is a super-hydrophobic bowl.
Optionally, the driving assembly of the droplet oscillation reaction part comprises four driving wheels connected with four driving rods, the four driving wheels are fixed on two rotating rods, synchronizing wheels are sleeved on the rotating rods, and a motor drives a synchronizing belt and the synchronizing wheels are driven by the synchronizing belts, so that the oscillation platform vibrates in a wave shape.
Optionally, the second connecting piece of the mechanical control assembly is L-shaped, and a cylindrical sliding cavity which is independent from top to bottom and is used for the inner cavity guide rod to slide respectively is arranged in the second connecting piece, and the miniature guide rail slideway is fixedly connected to the longitudinal outer side wall of the connecting piece.
Optionally, the connection ends of 4 of the outer circumference guide rods are provided with a bearing with a seat, and the bearing with a seat is fixed on the side frame of the main body frame.
Alternatively, the pipetting head of the micro-droplet transfer unit is made of a rivet which is subjected to super-hydrophobic treatment, and the dropper is fixed in the circular sleeve. The short rod penetrates through the opening of the circular sleeve and is pressed on the rubber head. The pressing plate is contacted with the short rod, and one end of the pressing plate can rotate. The rubber head can be extruded by pressing the pressing plate, and the whole transfer component is connected on the miniature guide rail to realize up-and-down sliding.
Optionally, two pressing plate guide rods are transversely and fixedly arranged at the lower part of the short rod of the outer wall of the sleeve.
Optionally, the main body frame is a rectangular parallelepiped frame made of aluminum, and a connecting surface between the frames is a transparent plate; wherein, two relative upper beams of main body frame are equipped with hinge and corresponding door-inhale respectively, and the transparent material face of upper surface can be followed one side and opened.
Optionally, still be equipped with the waste liquid temporary storage area on the operation platform for temporarily collect the waste liquid, save the experimental time.
The invention is more particularly described below: a desktop micro-droplet chemical reaction experiment platform based on a super-hydrophobic material comprises a micro-droplet bearing part, a micro-droplet transfer part, a temporary waste liquid storage area, a micro-droplet oscillation reaction part, a main body frame and a mechanical control assembly,
the core of the micro-droplet bearing part is a super-hydrophobic bowl-shaped utensil 1 and a super-hydrophobic bowl-shaped utensil 2 which are respectively placed on two drawing plates, and the drawing plates are placed in a drawing tray to realize the push-pull motion. The drawing tray is fixed on the acrylic plate material operating platform below.
The core of the micro-droplet oscillation reaction component III is a super-hydrophobic bowl-shaped vessel which is placed on an oscillation platform. The oscillating platform is a circular ring, the lower part of the oscillating platform is connected with the driving rods, the four driving rods are connected with the four driving wheels below the oscillating platform, the driving wheels are fixed on the two rotating rods, the synchronizing wheels are sleeved on the rotating rods, and the synchronous belts are driven by the motor and driven by the synchronous belts, so that the oscillating platform is in wavy vibration, and the purpose of uniformly mixing micro-droplets is achieved.
And the waste liquid temporary storage area is a waste liquid cylinder IV and is used for temporarily collecting waste liquid, so that the experimental time is saved.
The main body frame V is a cuboid formed by 12 aluminum profiles, and the aluminum profiles are fixed through triangular corner pieces by hexagon socket head cap bolts and T-shaped nut blocks. The handle is fixed respectively in frame top both sides, and as the relevant position installation hinge of door and door-inhale, the inside below of platform is the operation platform of an inferior gram force board material, uses the angle groove connecting piece to fix on the aluminium alloy.
The mechanical control assembly VI is structurally characterized in that four linear optical axis guide rods are fixed on an aluminum profile through bearings with seats, two linear optical axis guide rods form a cross shape and are connected with a square guide rod through a connecting part 26 and a connecting part 27, the connecting part is provided with a miniature guide rail capable of sliding up and down, a linear bearing is arranged between the connecting part 27 and the guide rail to enable the miniature guide rail to horizontally and freely slide along the guide rail, and the miniature guide rail is used for installing a micro-droplet transfer part.
Preferably, when the transparent acrylic plate is installed around the main body frame V, the interior of the platform is separated from the outside, the reaction liquid drops are thrown into the super-hydrophobic bowl-shaped utensil 1 and the super-hydrophobic bowl-shaped utensil 2 above the drawing plate by drawing out the drawing plate, and then the drawing plate is pushed back, so that the throwing of the reaction liquid drops can be realized.
Preferably, the mechanical control assembly VI is used for assisting the micro-droplet transfer part II to realize stable and accurate droplet position movement.
Preferably, the connection between the driving rod 12 and the oscillating platform 11 can be a flexible connection to provide smoother oscillation and better chemical reaction effect.
Preferably, the micro-droplet oscillation reaction part iii is driven by a stepping motor, and the stepping motor is controlled by changing a current signal, so that the oscillation time and the oscillation intensity can be adjusted, different oscillation schemes can be made for different reactions, and more kinds of micro-droplet reactions can be performed.
Preferably, the waste liquid cylinder can adopt a super-hydrophobic material, and the waste liquid can be transferred without damage. Meanwhile, the waste liquid cylinder is additionally provided with a cellular structure to store different batches of experimental waste liquid and prevent the waste liquid from mutually reacting.
Preferably, the super-hydrophobic bowl-shaped vessel placed on the oscillating platform is the same as or different from the super-hydrophobic bowl-shaped vessel 1 and the super-hydrophobic bowl-shaped vessel 2 on the drawing plate.
Preferably, the pipette head part of the dropper can grab liquid drops with different sizes by replacing rivets with different sizes, and the manufacturing methods of the super-hydrophobic surfaces of the rivets are consistent.
Preferably, the middle part of the pressing plate is connected with the bolt through a long groove-shaped hole.
Preferably, the dropper is made of a disposable dropper, and the dropper is reusable because the dropper is used according to the principle that the dropper provides the internal and external pressure difference and does not directly contact the liquid drops.
Preferably, a dropper in the micro-droplet transfer component II can be improved into a micro air pump at the later stage of the platform, so that the controllable automatic absorption and release of micro-droplets are realized; the method of program control and motor drive can be adopted in the late stage of the platform to realize the controllable automatic transfer and reaction of the micro-droplets.
The principle of the invention is as follows: the core of the micro-droplet receiving component is a low-density polyethylene super-hydrophobic bowl-shaped vessel, polyethylene does not react with most of water-soluble chemical reagents, the surface of the polyethylene bowl-shaped vessel is in a super-hydrophobic state, micro-droplets containing reaction reagents are non-wetting in the bowl-shaped vessel, and the micro-droplets are not adhered to the vessel when the micro-droplets are transferred; the micro-droplet transfer component is a super-hydrophobic aluminum rivet which is connected with the disposable plastic dropper, and the micro-droplet can be absorbed and released due to the change of air pressure and air flow in the plastic dropper caused by simple pressure-release operation. Because the surface of the rivet is a super-hydrophobic surface, the contact between the micro-droplet and the rivet is non-wetting contact, and the nondestructive absorption, transfer and release of the micro-droplet can be realized through the component; the micro-droplet oscillation reaction component drives the driving wheel and the driving rod to move through the motor, so that the oscillation platform and the super-hydrophobic bowl-shaped vessel on the oscillation platform vibrate in a waveform mode, and the micro-droplets after mixing periodically rotate and roll in the super-hydrophobic bowl-shaped vessel, so that the purposes of oscillation, mixing and acceleration of droplet reaction are achieved.
The invention also claims an experimental method using the micro-droplet chemical reaction experimental platform, which comprises the following steps:
1) and (3) putting micro droplets: micro liquid drops for reaction are respectively put on the hydrophobic cavities and are in a super-hydrophobic state;
2) transfer of micro-droplets: a. moving the micro-droplet transfer member in preparation for sucking the micro-droplets; b. half-pressing a pressing plate on the micro-droplet transfer component, moving downwards until the superhydrophobic surface of the dropper liquid transfer head is just in contact with the micro-droplets, slowly and slightly releasing the pressing plate, sucking the micro-droplets and lifting the micro-droplet transfer component upwards to suck the micro-droplets; c. the micro-droplet transfer component transfers the micro-droplets into a hydrophobic cavity of the micro-droplet oscillation reaction component; d. repeating the processes (a) and (b), sucking other micro-droplets, and transferring the micro-droplets to the upper part of the hydrophobic cavity; e. other micro-droplets are released and mixed with the micro-droplets in the hydrophobic cavity,
3) oscillation of the micro-droplets: and after the micro liquid drops used for reaction are thrown into a hydrophobic cavity on an oscillating platform through a micro liquid drop transfer component, the mixed liquid drops freely roll and oscillate on the super-hydrophobic surface.
Further, after the reaction is finished, the reacted micro-droplets are thrown into a waste liquid cylinder through a micro-droplet transfer component.
Further, in the step 1), the state of the hydrophobic cavity on the drawing plate before operation is dry and has no liquid drops; in the step 2), no liquid reagent residue is found in the super-hydrophobic liquid transfer head and the hydrophobic cavity before and after the transfer of the micro-droplets.
The technical scheme of the invention has the following advantages:
(1) the micro-droplet chemical reaction experimental platform mainly comprises a micro-droplet bearing part, a micro-droplet transfer part, a micro-droplet oscillation reaction part and a waste liquid temporary storage area, can complete a series of operations required by micro-droplet chemical reaction, and provides a space and a platform of a full flow for the micro-chemical reaction.
(2) The micro-droplet chemical reaction experiment platform is designed in a multifunctional and integrated mode, integrates a plurality of parts, performs optimization treatment, and improves the continuity of operation. The size of the platform can be increased or reduced according to actual conditions so as to increase or reduce internal components, and the platform has strong expansibility.
(3) The super-hydrophobic rivet and the super-hydrophobic bowl-shaped vessel used by the platform can be produced in a standardized manner, are quickly replaced and can be repeatedly used for a certain number of times.
(4) Due to the fact that the super-hydrophobic material is used, the material has self-cleaning property, and the platform can achieve multiple micro-droplet chemical reactions without cleaning. Meanwhile, the reaction liquid drop is small in size, waste liquid can be greatly reduced, and green and environment-friendly chemical reaction is realized.
(5) This desktop type micro-droplet chemical reaction experiment platform carries out chemical reaction through the micro-droplet for the required reagent of chemical reaction reduces by a wide margin, can greatly reduce the cost of partial chemical analysis and chemical detection.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic diagram of the overall structure of a chemical reaction experiment platform according to the present invention;
FIG. 2 is a schematic structural diagram of a micro-droplet receiving member of the chemical reaction experiment platform of the present invention;
FIG. 3 is a schematic structural diagram of a micro-droplet transfer unit of the chemical reaction experiment platform of the present invention;
FIG. 4 is a schematic structural diagram of a micro-droplet oscillation reaction part of the chemical reaction experiment platform of the present invention;
FIG. 5 is a schematic structural diagram of a main frame of the chemical reaction experiment platform of the present invention;
FIG. 6 is a schematic structural diagram of a mechanical control assembly of the chemical reaction experiment platform according to the present invention;
FIG. 7 is a schematic diagram of the operation process of the micro-droplet oscillation reaction of the chemical reaction experiment platform of the present invention;
in the figure: i-a micro-droplet bearing part, II-a micro-droplet transfer part, III-a micro-droplet oscillation reaction part, IV-a waste liquid cylinder, V-a main body frame, VI-a mechanical control component, 1-a super-hydrophobic bowl vessel, 2-a super-hydrophobic bowl vessel, 3-a drawing plate, 4-a drawing tray, 5-a liquid transferring head, 6-a dropper, 7-a round sleeve, 8-a short rod, 9-a pressing plate, 10-a super-hydrophobic bowl vessel, 11-an oscillation platform, 12-a driving rod, 13-a driving wheel, 14-a rotating rod, 15-a synchronous wheel, 16-a motor, 17-an aluminum profile, 18-a triangular corner piece, 19-a corner groove connecting piece, 20-a handle, 21-a hinge, 22-a door sucker and 23-an operation platform, 24-hexagon socket head cap screw, 25-linear optical axis guide rod, 26-connecting part, 27-connecting part, 28-bearing with seat, 29-micro guide rail and 30-linear bearing.
FIG. 8 is a diagram of a chemical reaction experiment platform illustrating a process of micro-droplet delivery;
in the figure: (a) a super-hydrophobic bowl-shaped vessel; (b) a micro-droplet putting process; (c) droplet receiving state.
FIG. 9 is a diagram of a chemical reaction experiment platform illustrating a droplet transfer process;
in the figure: (a) preparing to suck micro liquid drops; (b) absorbing micro-droplets; (c) transferring the micro-droplets to an oscillation reaction part; (d) preparing mixed micro-droplets; (e) mixing the micro-droplets; (f) mixed droplet enlargement.
FIG. 10 is a schematic diagram of the micro-droplet oscillation reaction process of the chemical reaction experiment platform of the present invention;
in the figure: (a) - (d) the location of the mixed droplets during oscillation; (e) the droplet state was mixed after the oscillation was completed.
FIG. 11 is a schematic diagram of the solution identification process of the chemical reaction experiment platform according to the present invention;
in the figure: (a1) - (a3) state before reaction of the micro-droplets; (b1) the state after the reaction of the- (b3) micro-droplets.
Detailed Description
The technical solution of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is obvious that the described experimental examples are a part of the experimental examples of the present invention, but not all of the experimental examples. Based on the experimental examples in the present invention, all other experimental examples obtained by a person of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.
Example 1
The micro-droplet chemical reaction experiment platform of the experimental example, as shown in fig. 1, includes: the device comprises a micro-droplet bearing part I, a micro-droplet transfer part II, a micro-droplet oscillation reaction part III, a waste liquid cylinder IV, a main body frame V and a mechanical control assembly VI.
The micro-droplet bearing part I is shown in figure 2, the core of the micro-droplet bearing part I is a super-hydrophobic bowl-shaped vessel 1, 2 which are respectively placed on two drawing plates 3, and the drawing plates 3 are placed in a drawing tray 4 to realize the push-pull movement. The drawing tray 4 is fixed on the acrylic plate material operating platform 23.
Micro-droplet transfer unit II referring to fig. 3, the pipetting head 5 is made of a rivet which is subjected to super-hydrophobic treatment, and the dropper 6 is made of a common disposable dropper in a laboratory, and the two are assembled together to form the core unit II. The dropper 6 is fixed inside the circular sleeve 7. The short rod 8 penetrates through the opening of the circular sleeve 7 and is pressed on the glue head. The pressing plate 9 is in contact with the short rod 8, and one end of the pressing plate can rotate. The rubber head can be extruded by pressing the pressing plate 9, and the whole transfer component is connected on the miniature guide rail 29 to realize up-and-down sliding. The liquid-transferring head 5 part of the dropper 6 can grasp liquid drops with different sizes by replacing rivets with different sizes, and the manufacturing methods of the super-hydrophobic surfaces of the rivets are all consistent. The dropper 6 is made of a disposable dropper, and the dropper 6 can be repeatedly used because the dropper 6 is used according to the principle that the internal and external pressure difference is provided and the dropper does not directly contact with liquid drops.
Further, a dropper 6 in the micro-droplet transfer component II can be improved into a micro air pump at the later stage of the platform, so that the controllable automatic absorption and release of the micro-droplets are realized; the method of program control and motor drive can be adopted in the late stage of the platform to realize the controllable automatic transfer and reaction of the micro-droplets.
The micro-droplet oscillation reaction component III is shown in figure 4, and the core of the component is a super-hydrophobic bowl 10 which is placed on an oscillation platform 11. The oscillating platform 11 is a circular ring, the lower part of the oscillating platform is connected with the driving rods 12, the four driving rods 12 are connected with the four driving wheels 13 below the oscillating platform, the driving wheels 13 are fixed on the two rotating rods 14, the synchronizing wheels 15 are sleeved on the rotating rods 14, and the synchronous belts are driven by the motors 16 and drive the synchronizing wheels 15, so that the oscillating platform 11 vibrates in a wavy manner to achieve the purpose of uniformly mixing micro-droplets. The connection between the driving rod 12 and the oscillating platform 11 can adopt flexible connection to provide smoother oscillation and better chemical reaction effect. The super-hydrophobic bowl-shaped vessel 10 placed on the oscillating platform 11 and the super-hydrophobic bowl-shaped vessel on the drawing plate 3 can be the same vessel. Furthermore, the micro-droplet oscillation reaction part III is driven by a stepping motor, the stepping motor is controlled by changing a current signal, the oscillation time and the oscillation intensity can be adjusted, different oscillation schemes can be made for different reactions, and more kinds of micro-droplet reactions can be performed.
And the waste liquid temporary storage area is a waste liquid cylinder IV and is used for temporarily collecting waste liquid, so that the experimental time is saved. Furthermore, the waste liquid cylinder can adopt a super-hydrophobic material, and the waste liquid can be transferred without damage. Meanwhile, the waste liquid cylinder is additionally provided with a cellular structure to store different batches of experimental waste liquid and prevent the waste liquid from mutually reacting.
The main body frame V is a cuboid composed of 12 aluminum profiles 17, and the aluminum profiles are fixed through triangular corner pieces 18 by hexagon socket head bolts 24 and T-shaped nut blocks, as shown in figure 5. A handle 20 is fixed on each side above the frame, a hinge 21 and a door stopper 22 are installed at corresponding positions of the door, an operation platform 23 made of acrylic plates is arranged below the inside of the platform, and the operation platform is fixed on an aluminum section bar through an angle groove connecting piece 19.
The mechanical control assembly VI is shown in figure 6, four linear optical axis guide rods 25 are fixed on an aluminum profile 17 through a bearing 28 with a base, the two linear optical axis guide rods 25 form a cross-shaped guide rod and a square guide rod and are connected through connecting parts 26 and 27, the connecting part 26 is provided with a miniature guide rail 29 capable of sliding up and down, a linear bearing 30 is arranged between the connecting part 27 and the guide rail so as to enable the miniature guide rail to horizontally and freely slide along the guide rail, and the miniature guide rail 29 is used for installing a micro-droplet transfer component. And the mechanical control assembly VI is used for assisting the micro-droplet transfer component II to realize stable and accurate droplet position movement.
Further, when the transparent acrylic plate is installed around the main body frame V, the interior of the platform is separated from the outside, the reaction liquid drops are thrown into the super-hydrophobic bowl-shaped utensils 1 and 2 above the drawing plate by drawing out the drawing plate 3, and then the drawing plate 3 is pushed back, so that the reaction liquid drops can be thrown in.
Experimental example 2
The micro-droplet chemical reaction experiment platform is a synthesis experiment implementation case, and the implementation case is a gold nanoparticle synthesis experiment.
The principle of the implementation case is as follows: after the reducing substance sodium borohydride is added into the oxidizing substance chloroauric acid, part of gold ions in the chloroauric acid solution are reduced into simple gold substances, after crystal nuclei are formed, the crystal nuclei grow, and all the gold ions are reduced into the simple gold substances to form gold sol, so that the gold nanoparticles are prepared. The generation of the gold nanoparticles can be proved by changing the color of the liquid drop from colorless to other colors.
1) And (3) putting micro droplets: a. before operation, the two super-hydrophobic bowl-shaped utensils 1 and 2 on the drawing plate 3 are in a dry state without liquid drops; b. the push-pull plate 3 is drawn out, the sodium borohydride micro-droplet is removed by a liquid removing gun and put on the super-hydrophobic bowl-shaped vessel 1 on the right side, and the sodium borohydride micro-droplet is in a super-hydrophobic state on the vessel; c. repeating the steps, and dropping chloroauric acid micro-droplets on the super-hydrophobic bowl-shaped vessel 2. After the liquid drops are put in, the two liquid drops are spherical and can freely roll when standing, both the two liquid drops are in a super-hydrophobic state, and the putting is finished.
Wherein the mass concentration of the sodium borohydride micro-droplets is 1.28 multiplied by 10-2mol/L, volume of 15 mu L, transparent and colorless color; the micro-droplet of the chloroauric acid has the mass concentration of 1.07 x 10-3mol/L, volume 15 μ L, light yellow color.
2) Transfer of micro-droplets: a. moving the micro-droplet transfer component II along the linear bearing 30 to prepare for absorbing the sodium borohydride micro-droplets; b. semi-pressing a pressing plate 9 on the micro-droplet transfer component II, moving downwards until the superhydrophobic surface of a liquid transfer head 5 of a dropper 6 is just in contact with the micro-droplets, slowly and slightly loosening the pressing plate 9, sucking the micro-droplets and lifting the micro-droplet transfer component II upwards, and sucking the sodium borohydride micro-droplets; c. the micro-droplet transfer component II transfers the sodium borohydride micro-droplets to the super-hydrophobic bowl-shaped vessel 10 at the micro-droplet oscillation reaction component III; d. repeating the processes (a) and (b), sucking the chloroauric acid micro-droplets, and transferring the chloroauric acid micro-droplets to the upper part of the sodium borohydride micro-droplets; e. and releasing the chloroauric acid micro-droplets, mixing the chloroauric acid micro-droplets with the sodium borohydride micro-droplets, changing the color of the micro-droplets from colorless to gray black, generating gold nanoparticles, and finding no liquid reagent residue in the super-hydrophobic liquid transfer head 5 and the super-hydrophobic bowl-shaped vessel before and after transferring the micro-droplets.
3) Oscillation of the micro-droplets: after two types of micro liquid drops are put into the super-hydrophobic bowl-shaped vessel 10 on the oscillating platform 11 through the micro liquid drop transfer component II, the motor 16 is started, and the mixed liquid drop of the chloroauric acid and the sodium borohydride freely rolls and oscillates on the super-hydrophobic surface. a. The micro-droplet oscillation reaction part III inclines to the left; b. the micro-droplet oscillation reaction part III inclines backwards; c. the micro-droplet oscillation reaction part III inclines rightwards; d. the micro-droplet oscillation reaction part III inclines forwards, the reaction droplets are in a spherical shape and roll freely periodically, and after the reaction is finished, the reacted micro-droplets are thrown into the waste liquid cylinder IV through the micro-droplet transfer part II; e. after shaking for 15-20 s, the color of the mixed liquid drop is changed into uniform gray black, and the color is not changed any more.
The experimental platform was based on the use of experimental example 1 above: the micro liquid drops can freely roll in the super-hydrophobic bowl-shaped vessel and are in a super-hydrophobic state; no liquid drop residue exists before and after the micro liquid drop transfer, and the lossless transfer can be realized; the mixed liquid drop is oscillated to change color, so that the gold nano particle synthesis reaction can be realized.
Experimental example 3
The embodiment is a potassium permanganate solution identification experiment.
The principle of the implementation case is as follows: the acidic potassium permanganate solution has strong oxidizing property, after completely reacting with the sodium oxalate solution, the heptavalent manganic acid radical ions are reduced into bivalent manganese ions, the color of the micro-droplets is changed from mauve to colorless, and the other two solutions react with the sodium oxalate solution and are changed into other colors.
1) And (3) putting the liquid to be detected and the detection liquid: and respectively putting the liquid drops to be detected and the liquid drops to be detected on the left side and the right side of the drawing plate 3 by using a liquid transfer gun.
The solution to be detected is potassium permanganate solution, acid fuchsin and sodium indigo disulfonate mixed solution, and alkaline fuchsin and sodium indigo disulfonate mixed solution in sequence, the volumes of the solutions are respectively 15 mu L, and the color is purple red.
The detection solution in the step 1) is a mixed solution of sodium oxalate and 1.56mol/L sulfuric acid, the mass concentration of which is 0.03mol/L, and the volume of the mixed solution is 15 mu L.
Experimental example 3 step 1) the details of the operation were the same as those in Experimental example 2 step 1).
2) Mixing the liquid to be detected with the detection liquid: firstly, a micro-droplet transfer component II is used for absorbing detection liquid into a super-hydrophobic bowl-shaped vessel 10 on a micro-droplet oscillation platform 11, and then the micro-droplet transfer component II is used for absorbing the detection liquid and mixing the detection liquid with the liquid to be detected.
Experimental example 3, step 2) the details of the operation were the same as those in Experimental example 2, step 2).
3) Oscillation of the liquid to be detected and the detection liquid: two types of micro liquid drops are thrown into a super-hydrophobic bowl-shaped vessel 10 on an oscillating platform 11 through a micro liquid drop transfer component II, a motor 16 is started, the mixed liquid drops freely roll and oscillate on the super-hydrophobic surface, and color change is observed after 4min, so that an identification conclusion is obtained.
Experimental example 3, step 3) the details of the operation were the same as those in Experimental example 2, step 3).
And step 3, the color change is as follows: magenta to colorless, magenta to red, magenta to light blue.
The identification conclusion in the step 3 is as follows: the No. 1 solution is a potassium permanganate solution, and the preset result is the same. The experimental platform is based on the use of experimental example 3: the micro liquid drops can freely roll in the bowl-shaped vessel and are in a super-hydrophobic state; no liquid drop residue exists before and after the micro liquid drop transfer, and the lossless transfer can be realized; the mixed liquid drop is oscillated to change color, so that the identification of the potassium permanganate solution can be realized.
It should be understood that the above experimental examples are only examples for clearly illustrating the present invention, and are not intended to limit the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.
Claims (10)
1. A micro-droplet chemical reaction experiment platform based on a super-hydrophobic material is characterized by comprising a micro-droplet bearing part, a micro-droplet transfer part, a micro-droplet oscillation reaction part, a main body frame and a mechanical control assembly,
the main body frame comprises a cavity and an operating platform arranged in the cavity;
the micro-droplet bearing part is fixed on the operating platform and is provided with one or more bearing pieces, and the bearing pieces are provided with drawable drainage cavities;
the micro-droplet oscillation reaction component is characterized in that an oscillation platform of the micro-droplet oscillation reaction component is arranged on the operation platform, and a hydrophobic cavity is arranged on the oscillation platform; the oscillating platform passes through a hole at the corresponding position of the operating platform through a driving rod rotatably connected with the lower surface of the oscillating platform and is connected with a driving component at the lower part of the oscillating platform, and the curved oscillation of the oscillating platform is realized through the lower driving component;
four linear optical axis guide rods of the mechanical control assembly are respectively arranged on the side surface of the main body frame to form an outer peripheral guide rod; the two linear optical axis guide rods are crossed into a cross shape and are arranged in the cavity of the main body frame to form inner cavity guide rods, the outer end parts of the inner cavity guide rods are connected with the outer periphery guide rods in a sliding mode through first connecting pieces, and the two inner cavity guide rods are connected with each other through second connecting pieces; a vertical micro guide rail is arranged on the second connecting piece;
the micro-droplet transfer component is connected with the micro guide rail in a sliding manner, so that the micro-droplet is transferred from the micro-droplet bearing component to the micro-droplet oscillation reaction component.
2. The experimental platform for micro droplet chemical reaction based on superhydrophobic material of claim 1, wherein the receiving member comprises a drawing tray fixedly connected to the edge of the gap of the operation platform, a slide is formed between the drawing tray and the lower portion of the outer edge of the gap of the operation platform, the drawing plate slides on the slide through the outer edge portions of two sides of the drawing plate, and the hydrophobic cavity is placed on the drawing plate.
3. The experimental platform for micro-droplet chemical reaction based on super-hydrophobic material as claimed in claim 1, wherein the driving assembly of the oscillating member comprises four driving wheels connected with four driving rods, the four driving wheels are fixed on two rotating rods, a synchronous wheel is sleeved on the rotating rods, and the synchronous belt is driven by a motor and driven by the synchronous wheel, so that the oscillating platform vibrates in wave shape.
4. The experimental platform for chemical reaction of micro-droplets based on super-hydrophobic material as claimed in claim 1, wherein the micro-droplet transfer unit comprises a liquid transfer head and a dropper, the dropper is fixed in the circular sleeve, the upper portion of the outer wall of the sleeve is provided with a short rod, the short rod passes through the hole on the side wall of the sleeve and presses on the inner rubber head, the bottom of the outer wall of the sleeve is provided with a rotating shaft, one end of the pressing plate is movably connected with the rotating shaft, the extending portion of the pressing plate contacts with the short rod, and the rubber head is pressed by rotating the pressing plate.
5. The experimental platform for micro-droplet chemical reaction based on super-hydrophobic material as claimed in claim 1, wherein a waste liquid temporary storage area is further provided on the operation platform.
6. The experimental platform for micro-droplet chemical reaction based on super-hydrophobic material as claimed in claim 1, wherein the micro-droplet oscillation reaction component is driven by a stepping motor, the stepping motor is controlled by changing current signal, the adjustment of oscillation time and oscillation intensity can be performed, different oscillation schemes can be made for different reactions, and more kinds of micro-droplet reactions can be performed.
7. The desktop type micro-droplet chemical reaction experiment platform based on the superhydrophobic material, according to claim 1, wherein the micro-droplet transfer component comprises a dropper, and controllable automatic absorption and release of micro-droplets are realized through a micro air pump.
8. An experimental method using the micro-droplet chemical reaction experimental platform according to any one of claims 1 to 7, comprising the steps of:
1) and (3) putting micro droplets: micro liquid drops for reaction are respectively put on the hydrophobic cavities and are in a super-hydrophobic state;
2) transfer of micro-droplets: a. moving the micro-droplet transfer member in preparation for sucking the micro-droplets; b. half-pressing a pressing plate on the micro-droplet transfer component, moving downwards until the superhydrophobic surface of the dropper liquid transfer head is just in contact with the micro-droplets, slowly and slightly releasing the pressing plate, sucking the micro-droplets and lifting the micro-droplet transfer component upwards to suck the micro-droplets; c. the micro-droplet transfer component transfers the micro-droplets into a hydrophobic cavity of the micro-droplet oscillation reaction component; d. repeating the processes (a) and (b), sucking other micro-droplets, and transferring the micro-droplets to the upper part of the hydrophobic cavity; e. other micro-droplets are released and mixed with the micro-droplets in the hydrophobic cavity,
3) oscillation of the micro-droplets: and after the micro liquid drops used for reaction are thrown into a hydrophobic cavity on an oscillating platform through a micro liquid drop transfer component, the mixed liquid drops freely roll and oscillate on the super-hydrophobic surface.
9. The experimental method according to claim 8, wherein after the reaction is completed, the reacted micro droplets are thrown into a waste liquid tank by a micro droplet transfer means.
10. The experimental method as claimed in claim 8, wherein in the step 1), the hydrophobic cavities on the drawing plate are dry and without liquid drops before operation; in the step 2), no liquid reagent residue is found in the super-hydrophobic liquid transfer head and the hydrophobic cavity before and after the transfer of the micro-droplets.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110026465.4A CN112827533B (en) | 2021-01-08 | 2021-01-08 | Desktop type micro-droplet chemical reaction experiment platform based on super-hydrophobic material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110026465.4A CN112827533B (en) | 2021-01-08 | 2021-01-08 | Desktop type micro-droplet chemical reaction experiment platform based on super-hydrophobic material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112827533A true CN112827533A (en) | 2021-05-25 |
| CN112827533B CN112827533B (en) | 2022-03-08 |
Family
ID=75929195
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110026465.4A Active CN112827533B (en) | 2021-01-08 | 2021-01-08 | Desktop type micro-droplet chemical reaction experiment platform based on super-hydrophobic material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112827533B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113426321A (en) * | 2021-08-27 | 2021-09-24 | 苏州汇涵医用科技发展有限公司 | High-efficient porous cellulose fermentation culture equipment |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100035273A1 (en) * | 2006-09-29 | 2010-02-11 | Ge Healthcare Bio-Sciences Ab | Method and device for small scale reactions |
| US20120009100A1 (en) * | 2010-07-12 | 2012-01-12 | Hamilton Bonaduz Ag | Pipette tip having a hydrophobic surface texture |
| CN102740978A (en) * | 2009-12-18 | 2012-10-17 | 扎芬纳股份公司 | Micropipette |
| CN104117396A (en) * | 2013-04-26 | 2014-10-29 | 中国科学院化学研究所 | Super-hydrophobic pipette gun head and preparation method thereof |
| CN105833814A (en) * | 2016-04-27 | 2016-08-10 | 浙江工业大学 | Method for manufacturing liquid drop self-driven microreactor and microreactor manufactured through method |
| CN106824317A (en) * | 2017-02-27 | 2017-06-13 | 重庆大学 | A kind of method that drop is manipulated using micro- electromagnetic wand |
| CN108593944A (en) * | 2018-03-22 | 2018-09-28 | 广州市第人民医院（广州消化疾病中心、广州医科大学附属市人民医院、华南理工大学附属第二医院） | Liquid drop chip immunoassay system and method |
| CN108722511A (en) * | 2018-08-09 | 2018-11-02 | 中国标准化研究院 | Draw the liquid-transfering gun pipette tips and preparation method thereof of thick liquid |
| CN208847750U (en) * | 2018-09-13 | 2019-05-10 | 青岛大学 | Micro syringe droplet based on super hydrophobic material precisely removes, delivery device |
| CN110354927A (en) * | 2019-07-18 | 2019-10-22 | 陕西科技大学 | A kind of extensive micro drop arrangement apparatus and method of gravity drive |
| WO2020237195A1 (en) * | 2019-05-22 | 2020-11-26 | The Regents Of The University Of California | Automated ultra-compact microdroplet radiosynthesizer |
-
2021
- 2021-01-08 CN CN202110026465.4A patent/CN112827533B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100035273A1 (en) * | 2006-09-29 | 2010-02-11 | Ge Healthcare Bio-Sciences Ab | Method and device for small scale reactions |
| CN102740978A (en) * | 2009-12-18 | 2012-10-17 | 扎芬纳股份公司 | Micropipette |
| US20120009100A1 (en) * | 2010-07-12 | 2012-01-12 | Hamilton Bonaduz Ag | Pipette tip having a hydrophobic surface texture |
| CN104117396A (en) * | 2013-04-26 | 2014-10-29 | 中国科学院化学研究所 | Super-hydrophobic pipette gun head and preparation method thereof |
| CN105833814A (en) * | 2016-04-27 | 2016-08-10 | 浙江工业大学 | Method for manufacturing liquid drop self-driven microreactor and microreactor manufactured through method |
| CN106824317A (en) * | 2017-02-27 | 2017-06-13 | 重庆大学 | A kind of method that drop is manipulated using micro- electromagnetic wand |
| CN108593944A (en) * | 2018-03-22 | 2018-09-28 | 广州市第人民医院（广州消化疾病中心、广州医科大学附属市人民医院、华南理工大学附属第二医院） | Liquid drop chip immunoassay system and method |
| CN108722511A (en) * | 2018-08-09 | 2018-11-02 | 中国标准化研究院 | Draw the liquid-transfering gun pipette tips and preparation method thereof of thick liquid |
| CN208847750U (en) * | 2018-09-13 | 2019-05-10 | 青岛大学 | Micro syringe droplet based on super hydrophobic material precisely removes, delivery device |
| WO2020237195A1 (en) * | 2019-05-22 | 2020-11-26 | The Regents Of The University Of California | Automated ultra-compact microdroplet radiosynthesizer |
| CN110354927A (en) * | 2019-07-18 | 2019-10-22 | 陕西科技大学 | A kind of extensive micro drop arrangement apparatus and method of gravity drive |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113426321A (en) * | 2021-08-27 | 2021-09-24 | 苏州汇涵医用科技发展有限公司 | High-efficient porous cellulose fermentation culture equipment |
| CN113426321B (en) * | 2021-08-27 | 2021-11-23 | 苏州汇涵医用科技发展有限公司 | High-efficient porous cellulose fermentation culture equipment |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112827533B (en) | 2022-03-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Vladisavljević et al. | Production of uniform droplets using membrane, microchannel and microfluidic emulsification devices | |
| Das et al. | Microfluidic-based photocatalytic microreactor for environmental application: a review of fabrication substrates and techniques, and operating parameters | |
| CN112827533B (en) | Desktop type micro-droplet chemical reaction experiment platform based on super-hydrophobic material | |
| Teh et al. | Droplet microfluidics | |
| JP7452896B2 (en) | Motion control mechanism microdroplet generation device | |
| EP3055055B1 (en) | Method for stirring at least one liquid | |
| CN108607518B (en) | Preparation method of manganese dioxide/graphene oxide-based self-driven micromotor | |
| Burger et al. | Rigorous buoyancy driven bubble mixing for centrifugal microfluidics | |
| CN109395788A (en) | A kind of intraluminal fluid dripping is for chip apparatus | |
| CN104998702A (en) | Preparation method of PDMS microfluidic chip based on liquid composite molding method | |
| CN214810926U (en) | Desktop type micro-droplet chemical reaction experiment platform based on super-hydrophobic material | |
| CN109622086B (en) | Microfluidic chip with preset magnetic beads, manufacturing method and microfluidic detection device | |
| CN215550117U (en) | Novel micro-plastic flotation separation device | |
| CN209362517U (en) | A kind of intraluminal fluid dripping is for chip structure | |
| Chen et al. | Surface-tension-confined droplet microfluidics☆ | |
| CN219272696U (en) | Micro-droplet preparation device | |
| CN206027541U (en) | Take cowl's filtration equipment for washing teeter chamber structure | |
| Kaneko et al. | A pumpless mixer for efficient capturing of small particles utilizing vibration-induced flow | |
| CN112266841A (en) | Biological sample processing chip device and processing method | |
| CN112604422A (en) | Industrial waste gas purifying equipment | |
| CN216499451U (en) | Microfluidic droplet generation chip device capable of being connected with injector | |
| TWM596764U (en) | Bioreactor and its stirring device | |
| CN114653268B (en) | Pneumatic micro-fluidic sample processing module of microballon vibrating | |
| CN218945793U (en) | Electronic multi-functional biological magnetic bead belt cleaning device | |
| CN112895122B (en) | Building stirring system and method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |