CN112816467A - Color developing agent for urine detection and application of color developing agent to paper diapers - Google Patents
Color developing agent for urine detection and application of color developing agent to paper diapers Download PDFInfo
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Abstract
The invention relates to the field of design and application research of urine developers. A color-developing agent for urine test, which has the following formula: KLuF4@KLu0.2Bi0.8I4. This patent embodies the colour developing agent of urine volume through directly acting on with the urine composition, has higher degree of accuracy, has fine application prospect in the panty-shape diapers field.
Description
Technical Field
The invention relates to the field of design and application research of urine developers, in particular to surface design, component regulation and control, compound preparation and detection performance research of a potassium bismuth iodide color developing agent for urine detection.
Background
The paper diaper is a daily article for babies, is convenient to use and can also ensure the health of the babies, is mostly provided with a urine display line, and judges whether the paper diaper needs to be replaced or not through the change of the color of the paper diaper. The common urine display types mainly comprise a hot-melt urine display adhesive and an ink urine display adhesive, wherein the former utilizes substances in the urine display adhesive to generate color reaction to the change of pH value so as to enable the paper diaper to show color change, and the latter utilizes urine to dilute colored small molecular substances so as to enable the color of a specific part of the paper diaper to disappear. [1-4] the two detection methods have large errors, such as the change of the environmental humidity and the scattered drinking water, etc., can cause the color change of the urine display material. Therefore, a more accurate detection method needs to be explored and applied to the production of novel diapers.
The main components of human urine include water, inorganic salts, organic substances and the like, wherein the inorganic substances include uric acid components. The methods for measuring uric acid mainly include phosphotungstic acid reduction method, uricase-peroxidase coupling method, uric acid sensor detection method and voltammetry [5-8 ]]All the methods need to be matched with specific instruments for use, and are not suitable for visually judging the using degree of the paper diapers. The molecular formula of uric acid is C through literature research5H4N4O3The molecular structure of the paper diaper contains a plurality of-NH bonds, the color developing agent is a color change which is directly shown through reaction with substances, and the common color developing agent cannot directly react with uric acid, so that the novel color developing agent which can react with uric acid is developed and applied to the paper diaper, and the detection accuracy can be improved.
The existing paper diaper with the urine display usually has one or two continuous lines, the urine display line is U-shaped when the paper diaper is used, when urine contacts the urine display line, the urine display line can change color or fade, whether the paper diaper is replaced is judged through the color change or fade position of the urine display line, and when only a little urine is wetted at the front end and the rear end of the urine display line, the urine display line can also change, so that the paper diaper is not beneficial to the judgment of a caregiver.
Reference to the literature
[1]L.B.Rubia,R.Gomez,Journal of Pharmaceutical Sciences,1977,66,1656-1657.
[2]N.Ferronato,M.L.N.Pinedo,V.Torretta,Sustainability,2020,12,5055.
[3]Y.Budyk,A.Fullana,Journal of Environmental Chmical Engineering,2019,7,103341.
[4]S.C.Khoo,X.Y.Phang,C.M.Ng,K.L.Lim,S.S.Lam,N.L.Ma,Process Safety and Ennironmental Protection,2019,123,116-129.
[5]A.B.Patil,C.B.Zheng,L.Y.Ma,R.H.Wu,S.K.Mengane,Y.F.Zhang,X.T.Liu,Z.H.Meng,W.L.Zhang,Z.J.Xu,C.F.Chen,J.N.Huang,X.Y.Liu,Nanotechnology,2021,32,065502.
[6]C.Y.Huang,H.C.Hsiao,Sensors,2020,20.
[7]C.Borghi,C.Bentivenga,E.R.Cosentino,International Journal of Cardiology,2020,320,23-24.
[8]H.Y.Zhang,S.Liu,Journal of Alloys and Compounds,2020,842,155873。
Disclosure of Invention
In order to solve the technical problems, the application aims to provide a novel polyacrylic acid, citric acid and Ethylene Diamine Tetraacetic Acid (EDTA) tri-ligand modified lutetium potassium fluoride-bismuth lutetium potassium iodide compound color developing agent, when the color developing agent encounters urine, hydroxyl (-OH) on the surface of the compound and-NH in urine molecules form a bond through electrostatic attraction, and then bismuth lutetium potassium iodide in the compound and uric acid in the urine react to form an organic-inorganic complex which is dark yellow and has brightness in direct proportion to the volume of the urine, so that the color developing agent can be well applied to quantitative detection of the urine; secondly, polyacrylic acid and citric acid in the surface ligand mutually act synergistically to promote electrostatic adsorption of bismuth lutetium potassium iodide and uric acid together; in addition, the ethylene diamine tetraacetic acid in the surface ligand is a chelating agent, can adsorb cations in urine, mainly sodium ions, and when the sodium ions are adsorbed to a certain concentration, the color developing agent is changed into orange. The interaction of the color developing agent and specific components in the urine is utilized to realize the detection of the urine, and the method has high accuracy and good application prospect in the application field of the paper diaper.
In order to achieve the above object, the present application adopts the following technical solutions:
a color-developing agent for urine test, which has the following formula: KLuF4@KLu0.2Bi0.8I4。
Further, the application provides a preparation method of the color developing agent for urine detection, which comprises the following steps:
1) dissolving (1-2) millimole lutetium acetate, (1-2) millimole potassium acetate and (4-8) millimole ammonium fluoride in (4-8) milliliter of deionized water, and stirring at room temperature for 30-60 minutes, wherein the molar ratio of lutetium acetate to potassium acetate to ammonium fluoride is 1:1: 4;
2) adding 20-30 ml of oleic acid and 10-20 ml of octadecene into the mixture in the step 1), stirring the mixture at room temperature for 30-60 minutes, transferring the mixture into a hydrothermal kettle, and preserving the heat at the temperature of 160-200 ℃ for 12-24 hours;
3) centrifugally washing the product obtained in the step 2) for 3-5 times by using a mixed solution of cyclohexane and ethanol (the volume ratio is 1:3), and then dispersing the obtained nanocrystal in (4-8) ml of cyclohexane;
4) adding 1-2 ml of dilute hydrochloric acid (1.6 mol/L) into the solution obtained in the step 3), carrying out ultrasonic treatment in an ultrasonic cleaning instrument with the power of more than 1 kilowatt for 30-60 minutes, then carrying out centrifugal washing (1-2) times by using a mixed solution of ethanol and deionized water (the volume ratio is 1:3), and dispersing the obtained nanocrystals in (2-4) ml of deionized water;
5) stirring (30-60) millimoles of basic bismuth nitrate (0.5-1), lutetium acetate (0.33-0.66), glacial acetic acid (5-20) and deionized water (40-60) at room temperature for 30-60 minutes, wherein the molar ratio of the bismuth nitrate to the lutetium acetate is 3: 2;
6) adding the nanocrystalline solution obtained in the step (4) into the solution obtained in the step 5), and stirring at room temperature for 30-60 minutes;
7) adding potassium iodide (4/5 molar weight of bismuth nitrate) and dilute hydrochloric acid (0.3-1 ml) into the solution obtained in the step 6), and stirring at room temperature for 10-20 minutes;
8) adding polyacrylic acid, citric acid and EDTA (the molar ratio is 1: (0.5-1): (0.2-0.4), the total molar amount of which is 1.5 times of the molar amount of the potassium iodide), stirring at room temperature for 2-5 minutes, then placing in an oven at 30-40 ℃ for 24-48 hours, and finally storing the obtained color developing agent in a black bottle.
Further, the application provides application of the color developing agent in quantitative detection of uric acid.
Further, the application provides an application of the color developing agent in infant urine detection.
The preparation method comprises the steps of firstly preparing lutetium potassium fluoride nanocrystalline by a solvothermal method, removing a surface ligand by hydrochloric acid treatment, then preparing a lutetium potassium fluoride-bismuth lutetium potassium iodide compound color developing agent by taking the nanocrystalline as a core and adopting a room-temperature stirring method, and then preparing the lutetium potassium fluoride-bismuth lutetium potassium iodide compound color developing agent coated with polyacrylic acid, citric acid and EDTA three ligands by surface modification. When the color developing agent is added with uric acid, the solution is dark yellow, the brightness degree of the solution is in direct proportion to the concentration of the uric acid, and the quantitative detection of the uric acid can be well applied by fitting a relation curve of the yellow light intensity and the concentration of the uric acid. On the basis, urine of a normal person is added into the lutetium potassium fluoride-bismuth lutetium potassium iodide color developing agent, yellow can be obviously seen, the brightness degree of the color developing agent is gradually deepened along with the increase of the volume of the urine, and after the volume of the urine reaches a certain degree, EDTA can adsorb enough sodium ions, and the color developing agent is changed into orange. This patent embodies the colour developing agent of urine volume through directly acting on with the urine composition, has higher degree of accuracy, has fine application prospect in the panty-shape diapers field.
Drawings
The inductively coupled plasma atomic emission spectrometry analysis results of the product of fig. 1.
Figure 2 fourier-ir transform spectrogram of the product.
FIG. 3 is a transmission electron micrograph of the product.
FIG. 4 shows yellow light intensity of the product as a function of uric acid concentration.
Figure 5 yellow light intensity of the product versus urine volume.
FIG. 6 is a curve showing the relationship between the yellow light intensity and the uric acid concentration of the color-developing agent of the surface ligand-free modified lutetium potassium fluoride-bismuth lutetium potassium iodide compound.
FIG. 7 is a graph showing the relationship between the yellow light intensity and the uric acid concentration of a single polyacrylic acid ligand modified lutetium potassium fluoride-bismuth lutetium potassium iodide composite color developing agent.
FIG. 8 is a curve of the relationship between the yellow light intensity and the uric acid concentration of the single citric acid ligand modified lutetium potassium fluoride-bismuth lutetium potassium iodide composite color developing agent.
FIG. 9 is a curve of the relationship between the yellow light intensity and the uric acid concentration of the EDTA ligand modified lutetium potassium fluoride-bismuth lutetium potassium iodide composite color-developing agent alone.
Detailed Description
Experimental part main reagents: potassium acetate (99.0%), ammonium fluoride (99.0%), bismuth subnitrate (98%), lutetium acetate (99.9%), potassium iodide (99.0%), glacial acetic acid (100%), oilAcid, octadecene (90%), polyacrylic acid (99%) and uric acid (99%) were purchased from Sigma-Aldrich, cyclohexane, dilute hydrochloric acid and deionized water were purchased from national pharmaceutical group chemicals, ltd, and test urine was taken from normal infants. KLuF4@KLu0.2Bi0.8I4Preparation of
Dissolving 1 mmol of lutetium acetate, 1 mmol of potassium acetate and 4 mmol of ammonium fluoride in 6 ml of deionized water, and stirring at room temperature for 30 minutes; then adding 20 ml of oleic acid and 10 ml of octadecene, stirring for 60 minutes at room temperature, then transferring to a hydrothermal kettle, and preserving heat for 12 hours at 160 ℃; KLuF4Centrifugally washing with a mixture of cyclohexane and ethanol (volume ratio of 1:3) for 3 times, and then adding KLuF4The nanocrystals were dispersed in 4 ml of cyclohexane; adding 1 ml of dilute hydrochloric acid into the solution, carrying out ultrasonic treatment in an ultrasonic cleaning instrument with the power of 1 kilowatt for 30 minutes, then carrying out centrifugal washing for 1 time by using a mixed solution of ethanol and deionized water (the volume ratio is 1:3), and carrying out KLuF (continuous phase refining) treatment4Dispersing the nanocrystals in 2 ml of deionized water; stirring 0.6 mmol of basic bismuth nitrate, 0.4 mmol of lutetium acetate, 10 ml of glacial acetic acid and 40 ml of deionized water at room temperature for 30 minutes to obtain a solution A; adding KLuF to solution A4Stirring the nanocrystalline solution at room temperature for 60 minutes; then 0.48 mmol potassium iodide and 0.3 ml dilute hydrochloric acid are added into the solution, and the mixture is stirred for 10 minutes at room temperature; polyacrylic acid, citric acid and EDTA (at a molar ratio of 1:0.8: 0.3) were then added, with a total molar amount of 0.72 mmol), stirred at room temperature for 5 minutes, then placed in an oven at 30 ℃ for 24 hours, and the resulting developer was finally stored in a black bottle.
Characterization instruments and methods inductively coupled plasma atomic emission spectroscopy (ZX-Z5000-WLD5000, powder samples, frequency 50/60Hz), Fourier Infrared transform spectroscopy (Nicolet 380), Transmission Electron microscopy (TEM, FEI Tecnai G2F 20), Spectroscopy (FLUROUB-B, HORIBA JOBIN YVON).
Preparation of atomic emission spectrometry test sample: freezing and drying the color developing agent to obtain a powdery sample;
preparation of Fourier infrared transform spectrum sample: the tablet is prepared by a superior pure KBr tablet method;
preparation of transmission electron microscope samples: dissolving a little developer in 4 ml of ethanol solution, and dropping 3-6 drops of liquid on the ultrathin carbon film after ultrasonic treatment for 5 minutes.
The detection method of uric acid comprises the following steps: removing color developing agents with certain volume, dividing the color developing agents into a plurality of groups, adding different molar amounts of uric acid into each group, representing the change of the fluorescence intensity by a fluorescence spectrometer, and fitting a standard curve.
The concentration of uric acid refers to the content of uric acid in a unit volume of aqueous solution, and the volume of urine refers to the total volume of urine.
Data analysis and discussion
As shown in FIG. 1, the result of atomic emission spectroscopy analysis of inductively coupled plasma shows that the polyacrylic acid ligand-modified bismuth lutetium potassium iodide product contains elements K, Lu, Bi, I and F, and the molar percentages of the elements K, Lu, Bi, I and F are 17.35%, 9.77%, 6.12%, 34.13% and 32.63%, respectively, and are substantially consistent with the molar percentage of the element added in the raw material, which indicates that the obtained product is KLuF4@KLu0.2Bi0.8I4. As shown in FIG. 2, the Fourier transform infrared spectroscopy test result shows that the surface of the product contains carboxyl functional groups, which are derived from polyacrylic acid, citric acid and EDTA ligand. The transmission electron microscope analysis result shows that the product is particles attached to the random sheet. The preparation process of the product comprises the following steps: firstly, potassium ions, lutetium ions and fluorine ions in the raw materials are combined to form KLuF4Then under KLuF4Forming bismuth lutetium potassium iodide on the surface, and then coating polyacrylic acid, citric acid and EDTA ligand through surface modification.
Polyacrylic acid, citric acid and EDTA three-ligand modified lutetium potassium fluoride-bismuth lutetium potassium iodide product is light white, and after a small amount of uric acid is added into a color developing agent, the color of the product is changed into yellow. The mechanism is explained as follows: carboxyl in the ligand and-NH bond in uric acid promote bismuth lutetium potassium iodide and uric acid to form a complex through electrostatic attraction, the complex is yellow finally, the concentration of the complex is increased along with the increase of the amount of uric acid, the color of the solution is gradually deepened, and the change amplitude can be characterized through fluorescence spectrum. As shown in FIG. 4, the intensity of yellow light is gradually enhanced with the continuous increase of the uric acid concentration, and a clear positive correlation is shown, so that the standard curve can be used for quantitative detection of uric acid. In addition, when the volume of urine reaches a certain content, the EDTA adsorbs excessive Na + ions, so that the color developing agent is changed into orange, and in order to further prove the practicability of the urine, the urine of normal infants is taken to continue to carry out a detection test.
As shown in figure 5, after a small amount of urine sample is added into polyacrylic acid, citric acid and EDTA three-ligand modified lutetium potassium fluoride-bismuth lutetium potassium iodide color developing agent, the color developing agent becomes yellow, the color of the color developing agent gradually deepens along with the further increase of the urine volume, the change trend of the color developing agent is in positive correlation with the urine volume, and the color developing agent can be used for urine detection as a standard curve. The color developing agent provided by the invention is used for manufacturing the paper diaper, can intuitively judge whether the paper diaper needs to be replaced or not according to the change of the color, and has good application prospect. In addition, when the urine reaches a certain critical volume, the EDTA adsorbs Na too much+Ion, developer turns orange, at which point the diaper must be replaced.
In order to verify the importance of modifying lutetium potassium fluoride-bismuth lutetium potassium iodide by adopting polyacrylic acid, citric acid and EDTA tri-ligand, the ligand-free modified lutetium potassium fluoride-bismuth lutetium potassium iodide color developing agent is prepared. As shown in fig. 6, no color change occurred with the addition of uric acid to this developer, and even when the amount of uric acid was increased to 1 micromole/liter, no color change occurred in the developer, indicating that the surface ligand helps to promote the binding of the developer and uric acid by surface action to form a complex. The adoption of the three-ligand modified color developing agent is an important innovation point of the invention.
In order to verify the effect of each ligand, polyacrylic acid, citric acid or EDTA mono-ligand modified lutetium potassium fluoride-bismuth lutetium potassium iodide compound is prepared respectively. As shown in the figures 7 and 8, when the polyacrylic acid or citric acid ligand modified lutetium potassium fluoride-bismuth lutetium potassium iodide color developing agent is added with uric acid, the yellow light intensity is increased slightly, and no color changes. This indicates that the dual ligands generate obvious synergistic effect, promote the formation of the complex by co-adsorbing uric acid, and result in significant change of luminous intensity.
As shown in FIG. 9, for EDTA single ligand modified lutetium potassium fluoride-bismuth lutetium potassium iodide color developing agent, the luminous intensity hardly changes with the change of uric acid concentration, and when excessive Na is added into the solution+After ionization, the developer turned orange, indicating that EDTA readily reacts with Na+The ions chelate, thereby changing the color of the developer.
In order to study the influence of different ratios of three ligands of acrylic acid, citric acid and EDTA on urine detection on the surface of the lutetium potassium fluoride-bismuth lutetium potassium iodide color developing agent, complexes with different surface ligand ratios were prepared, and the complexes comprise 1:0.5:0.2, 1:0.6:0.2, 1:0.7:0.2, 1:0.8:0.2, 1:0.9:0.2, 1:1:0.2, 1:0.8:0.3 and 1:0.8: 0.4. As shown in Table 1, 0.5X 10 was added to an equal volume (5 ml) of developer-3Millimole of uric acid, when the ratio is 1:0.8:0.3, the change of yellow light intensity before and after addition of uric acid is the largest, about 3.6 times.
TABLE 1
| Polyacrylic acid: citric acid: EDTA | Change of yellow light intensity before and after addition of uric acid |
| 1:0.5:0.2 | 1.6 |
| 1:0.6:0.2 | 2.1 |
| 1:0.7:0.2 | 2.7 |
| 1:0.8:0.2 | 3.2 |
| 1:0.9:0.2 | 3.1 |
| 1:1:0.2 | 2.8 |
| 1:0.8:0.3 | 3.6 |
| 1:0.8:0.4 | 3.3 |
The length of a urine display line of a common baby diaper is 200MM, and the change of the diaper is judged according to the color change or fading position of the urine display line, but the color depth change is not obvious. The urine display line provided by the invention has the advantages that the color depth change of the urine display line is very obvious, and whether the paper diaper needs to be replaced or not is judged intuitively and accurately according to the color depth. 10 identical urine development lines were prepared with a very small amount of color-developing agent, and 20 ml of the extracted urine from 10 normal infants was dropped on the urine development lines, respectively, and the change in yellow intensity was approximately 8000-. Furthermore, the change rule of the color intensity of the color developing agent can be adjusted by changing the concentration of the color developing agent on the urine development line, different types of paper diapers can be manufactured more flexibly according to different requirements, and in addition, when the color developing agent is changed into orange, the paper diapers are prompted to be replaced.
TABLE 2
Firstly, preparing lutetium potassium fluoride nanocrystalline by a solvothermal method, removing a surface ligand by hydrochloric acid treatment, then preparing a lutetium potassium fluoride-bismuth lutetium potassium iodide compound color developing agent by taking the nanocrystalline as a core and adopting a room-temperature stirring method, and then preparing the lutetium potassium fluoride-bismuth lutetium potassium iodide compound color developing agent coated by polyacrylic acid, citric acid and EDTA three ligands through surface modification. When the color developing agent is added with uric acid, the solution is dark yellow, the brightness degree of the solution is in direct proportion to the concentration of the uric acid, and the quantitative detection of the uric acid can be well applied by fitting a relation curve of the yellow light intensity and the concentration of the uric acid. On the basis, urine of a normal person is added into the lutetium potassium fluoride-bismuth lutetium potassium iodide color developing agent, yellow can be obviously seen, the brightness degree of the color developing agent is gradually deepened along with the increase of the volume of the urine, and after the volume of the urine reaches a certain degree, EDTA can adsorb enough sodium ions, the color developing agent is changed into orange, and the paper diaper is indicated to be replaced. Compared with the traditional pH detection method, the color developing agent which reflects the volume of the baby urine through the direct action of the color developing agent and urine components has higher accuracy and has good application prospect in the field of baby diapers.
A baby diaper comprises a diaper body and a urine display line sprayed on the diaper body, wherein the urine display line adopts the KLuF4@KLu0.2Bi0.8I4The prepared color developer. The length of the urine display line is not more than 100MM, the urine display line is straight, the urine display line is mainly positioned in the crotch region of the trousers body, and a small part of the urine display line extends to the front waist region and the rear waist region. Most of urine on the paper diaper is concentrated on the crotch area of the paper diaper, and especially when a user is in a standing state, most of urine near the front and rear waist areas above the crotch of the paper diaper also falls downwards into the crotch area, so that when the urine in the crotch area is pure too much, even if the urine near the front and rear waist areas above the crotch of the paper diaper is not much or not enough, the user can feel uncomfortable. Under this condition, traditional urine shows line because the urine of panty-shape diapers crotch portion top near back waist region shows the line and does not appear the suggestion, and the nurse personnel can't in time change, and uses the panty-shape diapers of this patent, only needs to observe the colour degree of depth that crotch portion regional urine shows the line and can judge the storage condition of urine, and then makes the accurate judgement whether change panty-shape diapers. The urine of this patent shows line and uses on being particularly suitable for pull-up diaper.
Claims (7)
1. A color developing agent for urine detection is characterized in thatThe molecular formula of the material is as follows: KLuF4@KLu0.2Bi0.8I4。
2. The reagent according to claim 1, wherein KLuF is a chromogenic reagent for urine detection4@KLu0.2Bi0.8I4The surface of the color developing agent is coated with polyacrylic acid, citric acid and EDTA.
3. The reagent according to claim 2, wherein the molar ratio of polyacrylic acid, citric acid and EDTA is 1: 0.5-1: 0.2-0.4.
4. The reagent according to claim 2, wherein the molar ratio of polyacrylic acid, citric acid and EDTA is 1:0.8: 0.3.
5. the method for preparing a developer for urine test according to claim 1, comprising the steps of:
1) dissolving (1-2) millimole lutetium acetate, (1-2) millimole potassium acetate and (4-8) millimole ammonium fluoride in (4-8) milliliter of deionized water, and stirring at room temperature for 30-60 minutes, wherein the molar ratio of lutetium acetate to potassium acetate to ammonium fluoride is 1:1: 4;
2) adding (20-30) ml of oleic acid and (10-20) ml of octadecene into the mixture in the step 1), stirring the mixture at room temperature for (30-60) minutes, transferring the mixture into a hydrothermal kettle, and performing hydrothermal reaction at 160 DEGoC-200oC, preserving heat for 12-24 hours;
3) centrifugally washing the product obtained in the step 2) for 3-5 times by using a mixed solution of cyclohexane and ethanol (the volume ratio is 1:3), and then dispersing the obtained nanocrystal in (4-8) ml of cyclohexane;
4) adding 1-2 ml of dilute hydrochloric acid (1.6 mol/L) into the solution obtained in the step 3), carrying out ultrasonic treatment in an ultrasonic cleaning instrument with the power of more than 1 kilowatt for 30-60 minutes, then carrying out centrifugal washing (1-2) times by using a mixed solution of ethanol and deionized water (the volume ratio is 1:3), and dispersing the obtained nanocrystals in (2-4) ml of deionized water;
5) stirring (30-60) millimoles of basic bismuth nitrate (0.5-1), lutetium acetate (0.33-0.66), glacial acetic acid (5-20) and deionized water (40-60) at room temperature for 30-60 minutes, wherein the molar ratio of the bismuth nitrate to the lutetium acetate is 3: 2;
6) adding the nanocrystalline solution obtained in the step (4) into the solution obtained in the step 5), and stirring at room temperature for 30-60 minutes;
7) adding potassium iodide (4/5 molar weight of bismuth nitrate) and dilute hydrochloric acid (0.3-1 ml) into the solution obtained in the step 6), and stirring at room temperature for 10-20 minutes;
8) adding polyacrylic acid, citric acid and EDTA (the molar ratio is 1: (0.5-1): (0.2-0.4) the total molar amount of which is 1.5 times of the molar amount of potassium iodide), stirring at room temperature for 2-5 minutes, and standing at 30-40oAnd C, standing in an oven for 24-48 hours, and finally storing the obtained color developing agent in a black bottle.
6. A paper diaper which is characterized by comprising a diaper body and a urine development line on the diaper body, wherein the urine development line is made of the color developing agent according to claim 1.
7. A diaper as claimed in claim 6, wherein the urine changes from pale white to yellow or orange after coming into contact with the urine at a line, and the brightness of yellow or orange is proportional to the volume of the urine.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110170809.9A CN112816467B (en) | 2021-02-08 | 2021-02-08 | Color developing agent for urine detection and application of color developing agent to paper diapers |
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| CN202110170809.9A CN112816467B (en) | 2021-02-08 | 2021-02-08 | Color developing agent for urine detection and application of color developing agent to paper diapers |
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