CN112816298A - Immunohistochemical slide fixer - Google Patents

Immunohistochemical slide fixer Download PDF

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Publication number
CN112816298A
CN112816298A CN202110145699.0A CN202110145699A CN112816298A CN 112816298 A CN112816298 A CN 112816298A CN 202110145699 A CN202110145699 A CN 202110145699A CN 112816298 A CN112816298 A CN 112816298A
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CN
China
Prior art keywords
slide
connecting rod
handle
positioning mechanism
rack
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Pending
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CN202110145699.0A
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Chinese (zh)
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韩晓霞
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Individual
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Individual
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Priority to CN202110145699.0A priority Critical patent/CN112816298A/en
Publication of CN112816298A publication Critical patent/CN112816298A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31Apparatus therefor
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813Producing thin layers of samples on a substrate, e.g. smearing, spinning-on

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention discloses an immunohistochemical slide fixer, which comprises a bottom plate, a fixing mechanism and a positioning mechanism; a bottom plate: the lower surface of the rotary plate is provided with a non-slip mat, the left side of the upper surface of the bottom plate is provided with a stand column, the upper surface of the stand column is provided with a rotary shaft seat, the left side surface of the rotary shaft seat is provided with a fixing clamp, the rear side surface of the rotary shaft seat is provided with a transverse U-shaped plate, and the inside of the rotary shaft seat is rotatably connected with a rectangular connecting rod; a fixing mechanism: the movable clamp is arranged in the U-shaped plate, the front side surface of the fixing mechanism is provided with the movable clamp, and the movable clamp and the fixing clamp are both internally provided with a silica gel sleeve; a positioning mechanism: set up in the surface of rectangle connecting rod, positioning mechanism's right flank is equipped with the handle, and fixed establishment includes slide rail, rack, fixed plate and little spring, and the slide rail sets up in the inside left side of U template, and sliding connection has the rack between two slide rails, this special slide fixer of immunohistochemistry, and the mark piece is fixed firm, and convenient to use.

Description

Immunohistochemical slide fixer
Technical Field
The invention relates to the technical field of medical instruments, in particular to an immunohistochemical slide fixer.
Background
Immunohistochemistry, which is the principle of antigen-antibody reaction based on the basic principle of immunology, i.e. the principle of specific binding of antigen and antibody, uses chemical reaction to make color development agent (fluorescein, enzyme, metal ion, isotope) of labeled antibody to determine the antigen (polypeptide and protein) in tissue cell, and makes positioning, qualitative and relatively quantitative research on the antigen, which is called immunohistochemistry technology, at present, when conducting immunohistochemical staining experiment, the tissue specimen adhered on the glass slide needs to be completely immersed in experimental solution (such as hydrogen peroxide methanol, hematoxylin staining solution, xylene, absolute ethyl alcohol, etc.) to gradually operate, and also needs to be cleaned, because there is no special glass slide fixer, the experimenter needs to take and put the glass slide with tweezers, the tweezers are very easy to slide down to damage the specimen, because these solvent parts contain toxicity, the hand-held operation is easy to touch the solution to harm the health of the operator, the tweezers are clamped and still need follow the new experiment after slipping, cause experimental efficiency not high, and increased experimenter's work burden to the tweezers are clamped the angular adjustment and are not enough nimble use comparatively troublesome.
Disclosure of Invention
The invention aims to overcome the defects in the prior art, provides an immunohistochemical slide fixer, is firm and reliable in slide fixing, is convenient for adjusting the angle of a handle, and can effectively solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: the immunohistochemical slide fixer comprises a bottom plate, a fixing mechanism and a positioning mechanism;
a bottom plate: the lower surface of the rotary plate is provided with a non-slip mat, the left side of the upper surface of the bottom plate is provided with a stand column, the upper surface of the stand column is provided with a rotary shaft seat, the left side surface of the rotary shaft seat is provided with a fixing clamp, the rear side surface of the rotary shaft seat is provided with a transverse U-shaped plate, and the inside of the rotary shaft seat is rotatably connected with a rectangular connecting rod;
a fixing mechanism: the movable clamp is arranged in the U-shaped plate, the front side surface of the fixing mechanism is provided with the movable clamp, and the movable clamp and the fixing clamp are both internally provided with a silica gel sleeve;
a positioning mechanism: set up in the surface of rectangle connecting rod, positioning mechanism's right flank is equipped with the handle, can utilize the parallel clamp of elasticity of spring to get the slide, guarantees that the slide atress is even, and can not crowd garrulous slide to the angular adjustment of handle is convenient, and what the handle transferred into when soaking is vertical conveniently to be soaked, and the handle transferred into horizontal when washing conveniently washes, has alleviateed chemical examination personnel's work burden, and is favorable to improving chemical examination efficiency.
Further, fixed establishment includes slide rail, rack, fixed plate and little spring, the slide rail sets up in the inside left side of U template, and sliding connection has the rack between two slide rails, and the left surface of rack is equipped with the removal and presss from both sides, and the fixed plate sets up in the trailing flank of U template, is equipped with little spring between the leading flank of fixed plate and the trailing flank that the removal pressed from both sides, utilizes the elasticity fixed slide of spring, can prevent crowded garrulous slide.
Further, fixed establishment still includes center pin, gear and dials the handle, the center pin sets up in the inside of U template, and the surface middle part of center pin is equipped with the gear, and the gear is connected with the rack toothing, and the surface of gear is equipped with dials the handle, can make things convenient for the drive to remove and press from both sides and move to the right, makes things convenient for fixed slide.
Further, positioning mechanism includes slide shaft, separation blade and big spring, the slide shaft sets up in the right flank of rectangle connecting rod, and the surface left side sliding connection of rectangle connecting rod has the separation blade, and big spring activity cup joints in the surface of rectangle connecting rod, and big spring is located between the left surface of slide shaft and the right flank of separation blade, and the left surface of separation blade and the right flank contact of pivot seat, the right flank of slide shaft are equipped with the handle, can restrict the rotation of rectangle connecting rod, guarantee the angle facilitate use of handle.
Furthermore, the positioning mechanism further comprises a connecting sheet, a sliding ring and a shifting column, the sliding ring is connected to the outer surface of the sliding shaft in a sliding mode, the connecting sheet is symmetrically arranged between the sliding ring and the blocking sheet, and the shifting column is arranged on the outer surface of the sliding ring, so that the rectangular connecting rod can be conveniently unlocked.
Compared with the prior art, the invention has the beneficial effects that: the special slide fixer for immunohistochemistry has the following advantages:
1. when a glass slide is fixed, the handle is held, the shifting handle is pulled to the inner side relatively, the shifting handle drives the gear to rotate clockwise around the axis of the central shaft, the gear rotates clockwise to drive the rack which is meshed and connected to slide to the rear side under the guidance of the slide rail, the slide rail drives the movable clamp to move backwards, the movable clamp compresses a small spring, the movable clamp moves backwards to drive the silica gel sleeve on the rear side to move backwards, the distance between the two silica gel sleeves is larger than the width of the glass slide, at the moment, the glass slide is placed between the two silica gel sleeves by tweezers, after the glass slide is placed, the shifting handle is loosened by hands, the compressed small spring drives the movable clamp to move forwards under the support of the fixed plate, the movable clamp moves forwards to drive the silica gel sleeve to move forwards, the two silica gel sleeves fix the glass slide under the support of the movable clamp and the fixed clamp, so that the glass slide can be clamped in, the efficiency of the assay is improved.
2. When the tissue specimen needs to be soaked in the test solution, the handle is held by a hand to shift the shifting post rightwards, the shifting post drives the sliding ring to move rightwards along the sliding shaft, the sliding ring drives the blocking piece to move rightwards through the connecting piece, the blocking piece moves rightwards to compress the large spring and is separated from the right side surface of the U-shaped plate, the blocking piece cannot block the rotation of the rectangular connecting rod at the moment, the handle rotates anticlockwise around the axis of the central rotating shaft of the rotating shaft seat, the handle drives the positioning mechanism and the rectangular connecting rod to rotate ninety degrees, the axis of the handle is vertical to the upper surface of the slide at the moment, the shifting post is loosened by the hand, the blocking piece is driven to move downwards by the compressed large spring, the lower surface of the blocking piece is attached to the upper surface of the rotating shaft seat, the blocking piece can prevent the rectangular connecting rod from rotating, the handle can be lifted at, the handle is transferred into horizontal convenient washing when washing, can be convenient handle the tissue sample on the slide to can effectually stop assay solution and spill onto assay personnel's health, protected assay personnel's healthy.
Drawings
FIG. 1 is a schematic view of the structure of the present invention.
In the figure: the device comprises a base plate 1, a non-slip mat 2, a column 3, a rotating shaft seat 4, a fixing clamp 5, a U-shaped plate 6, a fixing mechanism 7, a sliding rail 71, a rack 72, a fixing plate 73, a small spring 74, a central shaft 75, a gear 76, a shifting handle 77, a rectangular connecting rod 8, a positioning mechanism 9, a sliding shaft 91, a blocking piece 92, a large spring 93, a connecting sheet 94, a sliding ring 95, a shifting column 96, a handle 10, a movable clamp 11 and a silica gel sleeve 12.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1, the present invention provides a technical solution: the immunohistochemical slide fixer comprises a bottom plate 1, a fixing mechanism 7 and a positioning mechanism 9;
bottom plate 1: the lower surface of the base plate is provided with a non-slip mat 2, the base plate 1 provides a support and mounting position for an upper mechanism, the non-slip mat 2 increases the friction between a fixer and a desktop, the left side of the upper surface of the base plate 1 is provided with an upright post 3, the upright post 3 provides a support for the upper mechanism, the upper surface of the upright post 3 is provided with a rotating shaft seat 4, the left side surface of the rotating shaft seat 4 is provided with a fixing clamp 5, the rear side surface of the rotating shaft seat 4 is provided with a transverse U-shaped plate 6, the U-shaped plate 6 provides a mounting position for a point position mechanism, and the inside of the rotating shaft;
the fixing mechanism 7: the fixing mechanism 7 comprises a slide rail 71, a rack 72, a fixing plate 73 and a small spring 74, the slide rail 71 is arranged on the left side of the interior of the U-shaped plate 6, the rack 72 is connected between the two slide rails 71 in a sliding manner, the moving clamp 11 is arranged on the left side of the rack 72, the fixing plate 73 is arranged on the rear side of the U-shaped plate 6, the small spring 74 is arranged between the front side of the fixing plate 73 and the rear side of the moving clamp 11, the fixing mechanism 7 further comprises a central shaft 75, a gear 76 and a shifting handle 77, the central shaft 75 is arranged in the U-shaped plate 6, the gear 76 is arranged in the middle of the outer surface of the central shaft 75, the gear 76 is meshed with the rack 72, the shifting handle 77 is arranged on the outer surface of the gear 76, the handle 10 is held when a slide is fixed, the shifting handle 77 is relatively pulled inwards, the shifting handle 77 drives the gear 76 to rotate clockwise around the axis of the central shaft 75, the gear 76 rotates clockwise to drive the rack 72 which is meshed and connected to slide to the rear side under the guidance of the sliding rail 71, the sliding rail 71 drives the movable clamp 11 to move backwards, the movable clamp 11 compresses the small spring 74, the movable clamp 11 moves backwards to drive the silica gel sleeve 12 on the rear side to move backwards, the distance between the two silica gel sleeves 12 is larger than the width of a slide, at the moment, the slide is placed between the two silica gel sleeves 12 by using tweezers, after the slide is placed, the shifting handle 77 is loosened by hand, the compressed small spring 74 drives the movable clamp 11 to move forwards under the support of the fixed plate 73, the movable clamp 11 moves forwards to drive the silica gel sleeves 12 to move forwards, and the two silica gel sleeves 12 fix the slide under the support of the movable clamp 11;
the positioning mechanism 9: the positioning mechanism 9 comprises a sliding shaft 91, a blocking piece 92 and a large spring 93, the sliding shaft 91 is arranged on the right side surface of the rectangular connecting rod 8, the blocking piece 92 is connected to the left side of the outer surface of the rectangular connecting rod 8 in a sliding manner, the large spring 93 is movably sleeved on the outer surface of the rectangular connecting rod 8, the large spring 93 is positioned between the left side surface of the sliding shaft 91 and the right side surface of the blocking piece 92, the left side surface of the blocking piece 92 is in contact with the right side surface of the rotating shaft seat 4, the handle 10 is arranged on the right side surface of the sliding shaft 91, the positioning mechanism 9 further comprises a connecting piece 94, a sliding ring 95 and a poking column 96, the sliding ring 95 is connected to the outer surface of the sliding shaft 91 in a sliding manner, the connecting piece 94 is symmetrically arranged between the sliding ring 95 and the blocking piece 92, the poking column 96 is arranged on the outer surface of the sliding ring 95, when the tissue sample needs to be soaked in an, slip ring 95 passes through connection piece 94 and drives separation blade 92 and move to the right, separation blade 92 moves to the right with big spring 93 of compression and with the right flank separation of U template 6, separation blade 92 will not obstruct the rotation of rectangular connecting rod 8 this moment, the anticlockwise handle 10 that rotates of axle center of 4 central pivots of revolute shaft seat, handle 10 drives positioning mechanism 9 and the rotatory ninety degrees of rectangular connecting rod 8, the axle center of handle 10 and the upper surface of slide this moment are perpendicular, the hand is loosened and is dialled post 96, the big spring 93 drive separation blade 92 of compression moves down, the lower surface of separation blade 92 and the upper surface laminating of pivot seat 4, separation blade 92 will prevent the rotation of rectangular connecting rod 8, handle 10 can be carried forward this moment, can conveniently soak the tissue sample on the slide to the chemical examination solution in.
When in use: when fixing the slide, holding the handle 10, pulling the toggle handle 77 relatively inward, the toggle handle 77 driving the gear 76 to rotate clockwise around the axis of the central shaft 75, the gear 76 rotating clockwise driving the rack 72 engaged and connected to slide to the rear side under the guidance of the slide rail 71, the slide rail 71 driving the movable clamp 11 to move backward, the movable clamp 11 compressing the small spring 74, the movable clamp 11 moving backward driving the silica gel sleeve 12 at the rear side to move backward, the distance between the two silica gel sleeves 12 is larger than the width of the slide, at this time, placing the slide between the two silica gel sleeves 12 with tweezers, after placing, releasing the toggle handle 77 by hand, at this time, the compressed small spring 74 driving the movable clamp 11 to move forward under the support of the fixed plate 73, the movable clamp 11 moving forward driving the silica gel sleeves 12 to move forward, the two silica gel sleeves 12 fixing the slide under the support of the movable clamp 11 and the fixed clamp 5, attaching the tissue surface to the upper surface of the slide, the slide can be moved by hand holding the, when the tissue specimen needs to be soaked in the test solution, the handle 10 is held by a hand to shift the shifting post 96 rightwards, the shifting post 96 drives the sliding ring 95 to move rightwards along the sliding shaft 91, the sliding ring 95 drives the blocking piece 92 to move rightwards through the connecting sheet 94, the blocking piece 92 moves rightwards to compress the large spring 93 and is separated from the right side face of the U-shaped plate 6, at the moment, the blocking piece 92 cannot block the rotation of the rectangular connecting rod 8, the handle 10 rotates anticlockwise around the axis of the central rotating shaft of the rotating shaft seat 4, the handle 10 drives the positioning mechanism 9 and the rectangular connecting rod 8 to rotate ninety degrees, at the moment, the axis of the handle 10 is perpendicular to the upper surface of the slide, the shifting post 96 is loosened by the hand, the compressed large spring 93 drives the blocking piece 92 to move downwards, the lower surface of the blocking piece 92 is attached to the upper surface of the rotating shaft seat 4, the blocking piece 92 can block.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (5)

1. Immunohistochemical slide fixer which characterized in that: comprises a bottom plate (1), a fixing mechanism (7) and a positioning mechanism (9);
base plate (1): the lower surface of the rotary table is provided with a non-slip mat (2), the left side of the upper surface of the bottom plate (1) is provided with an upright post (3), the upper surface of the upright post (3) is provided with a rotary shaft seat (4), the left side surface of the rotary shaft seat (4) is provided with a fixing clamp (5), the rear side surface of the rotary shaft seat (4) is provided with a transverse U-shaped plate (6), and the inside of the rotary shaft seat (4) is rotatably connected with a rectangular connecting rod (8);
fixing mechanism (7): the fixing mechanism is arranged in the U-shaped plate (6), the front side surface of the fixing mechanism (7) is provided with a movable clamp (11), and silica gel sleeves (12) are arranged in the movable clamp (11) and the fixing clamp (5);
positioning mechanism (9): is arranged on the outer surface of the rectangular connecting rod (8), and the right side surface of the positioning mechanism (9) is provided with a handle (10).
2. The immunohistochemical slide holder of claim 1, wherein: fixed establishment (7) include slide rail (71), rack (72), fixed plate (73) and little spring (74), slide rail (71) set up in the inside left side of U template (6), and sliding connection has rack (72) between two slide rails (71), and the left surface of rack (72) is equipped with to remove presss from both sides (11), and fixed plate (73) set up in the trailing flank of U template (6), is equipped with little spring (74) between the leading flank of fixed plate (73) and the trailing flank that removes clamp (11).
3. The immunohistochemical slide holder of claim 2, wherein: the fixing mechanism (7) further comprises a central shaft (75), a gear (76) and a shifting handle (77), the central shaft (75) is arranged inside the U-shaped plate (6), the gear (76) is arranged in the middle of the outer surface of the central shaft (75), the gear (76) is meshed with the rack (72) and connected, and the shifting handle (77) is arranged on the outer surface of the gear (76).
4. The immunohistochemical slide holder of claim 1, wherein: positioning mechanism (9) are including slide shaft (91), separation blade (92) and big spring (93), slide shaft (91) set up in the right flank of rectangle connecting rod (8), and the surface left side sliding connection of rectangle connecting rod (8) has separation blade (92), and big spring (93) activity is cup jointed in the surface of rectangle connecting rod (8), and big spring (93) are located between the left surface of slide shaft (91) and the right flank of separation blade (92), the left surface of separation blade (92) and the right flank contact of pivot seat (4), and the right flank of slide shaft (91) is equipped with handle (10).
5. The immunohistochemical slide holder of claim 4, wherein: positioning mechanism (9) still include connection piece (94), sliding ring (95) and group post (96), sliding ring (95) sliding connection is in the surface of slide shaft (91), and the symmetry is equipped with connection piece (94) between sliding ring (95) and separation blade (92), and the surface of sliding ring (95) is equipped with group post (96).
CN202110145699.0A 2021-02-03 2021-02-03 Immunohistochemical slide fixer Pending CN112816298A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110145699.0A CN112816298A (en) 2021-02-03 2021-02-03 Immunohistochemical slide fixer

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110145699.0A CN112816298A (en) 2021-02-03 2021-02-03 Immunohistochemical slide fixer

Publications (1)

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CN112816298A true CN112816298A (en) 2021-05-18

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CN202110145699.0A Pending CN112816298A (en) 2021-02-03 2021-02-03 Immunohistochemical slide fixer

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CN (1) CN112816298A (en)

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040040594A (en) * 2002-11-07 2004-05-13 (주)로봇앤드디자인 Slide gripper for dna scanner
US20040114227A1 (en) * 2001-03-15 2004-06-17 Henderson Chester John Slide holder for an automated slide loader
US20080317566A1 (en) * 2006-08-04 2008-12-25 Ikonisys, Inc. Automated Cassette and Slide Handling System for an Automatic Microscope
CN102313978A (en) * 2010-06-30 2012-01-11 索尼公司 Stage system and microscope
US20170322407A1 (en) * 2014-05-29 2017-11-09 Rarecyte, Inc. Automated substrate loading
CN207807717U (en) * 2017-12-27 2018-09-04 广州蓝勃生物科技有限公司 A kind of slide clamping device of cover plate instrument
CN211043794U (en) * 2019-12-27 2020-07-17 天津英瑞达医疗科技发展有限公司 Slide glass clamping device of microscope objective table
CN212159274U (en) * 2020-04-29 2020-12-15 上海皓信生物科技有限公司 Automatic change pelleter's cover plate structure

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040114227A1 (en) * 2001-03-15 2004-06-17 Henderson Chester John Slide holder for an automated slide loader
KR20040040594A (en) * 2002-11-07 2004-05-13 (주)로봇앤드디자인 Slide gripper for dna scanner
US20080317566A1 (en) * 2006-08-04 2008-12-25 Ikonisys, Inc. Automated Cassette and Slide Handling System for an Automatic Microscope
CN102313978A (en) * 2010-06-30 2012-01-11 索尼公司 Stage system and microscope
US20170322407A1 (en) * 2014-05-29 2017-11-09 Rarecyte, Inc. Automated substrate loading
CN207807717U (en) * 2017-12-27 2018-09-04 广州蓝勃生物科技有限公司 A kind of slide clamping device of cover plate instrument
CN211043794U (en) * 2019-12-27 2020-07-17 天津英瑞达医疗科技发展有限公司 Slide glass clamping device of microscope objective table
CN212159274U (en) * 2020-04-29 2020-12-15 上海皓信生物科技有限公司 Automatic change pelleter's cover plate structure

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