CN112782320B - Method for identifying bupleurum chinense and Tibetan bupleurum chinense and application thereof - Google Patents
Method for identifying bupleurum chinense and Tibetan bupleurum chinense and application thereof Download PDFInfo
- Publication number
- CN112782320B CN112782320B CN202110204917.3A CN202110204917A CN112782320B CN 112782320 B CN112782320 B CN 112782320B CN 202110204917 A CN202110204917 A CN 202110204917A CN 112782320 B CN112782320 B CN 112782320B
- Authority
- CN
- China
- Prior art keywords
- bupleurum chinense
- mxt
- bupleurum
- tibetan
- chromatographic column
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/86—Signal analysis
- G01N30/8624—Detection of slopes or peaks; baseline correction
- G01N30/8631—Peaks
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/86—Signal analysis
- G01N30/8675—Evaluation, i.e. decoding of the signal into analytical information
- G01N30/8686—Fingerprinting, e.g. without prior knowledge of the sample components
Landscapes
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Library & Information Science (AREA)
- Engineering & Computer Science (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention discloses a method for identifying bupleurum chinense and Tibetan bupleurum chinense and application thereof, which comprises the following steps: and (3) taking a radix bupleuri sample, incubating for 30-40min, and detecting by using headspace-gas chromatography-ion mobility spectrometry. Wherein, MXT-WAX chromatographic column is used for detecting and obtaining 4 volatility characteristic peaks of bupleurum chinense, 4 volatility characteristic peaks of Tibetan bupleurum chinense, and the total ratio of PC-1 and PC-2 in the principal component analysis is 91%. Compared with the prior art, the identification method provided by the invention has the advantages of no need of sample pretreatment, short analysis period, low detection cost and portability of the instrument.
Description
Technical Field
The invention relates to an identification method of traditional Chinese medicinal materials, in particular to a method for identifying bupleurum chinense and Tibetan bupleurum chinense and application thereof.
Background
Bupleurum chinense (Bupleurum chinense) is dried root of Bupleurum chinense (Bupleurum chinense) of Umbelliferae, is one of the most commonly used exterior-syndrome-relieving medicines in clinic, has pungent and bitter taste, belongs to liver, gallbladder and lung channels, has the effects of soothing liver, relieving depression, relieving muscles, reducing fever and lifting yang qi, and is used for treating symptoms such as cold, fever, alternating chills and fever, malaria, stagnation of qi due to depression of the liver and the like. Tibetan radix bupleuri (B.marginatum var. Stenophyllum.) is mainly produced in Gansu, has similar antipyretic and anti-inflammatory effects to that of northern radix bupleuri, but has no obvious liver protection effect and pharmacological toxicity.
The bupleurum has various chemical components, and different genuine medicinal materials have different contents of effective components, which directly relate to the clinical curative effect. In the related art, most methods for identifying bupleurum are character identification, such as Thin Layer Chromatography (TLC), High Performance Liquid Chromatography (HPLC), high performance liquid chromatography-mass spectrometry (HPLC-MS) and the like, but character identification is greatly influenced by subjective factors, and methods such as TCL, HPLC-MS and the like require complex pretreatment of medicinal materials and have long analysis period.
Therefore, establishing a rapid and efficient method for identifying Tibetan radix bupleuri and northern radix bupleuri is of great significance.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art described above. Therefore, the invention provides a method for identifying bupleurum chinense and Tibetan bupleurum chinense, which directly detects the bupleurum chinense and Tibetan bupleurum chinense by using a headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) technology, quickly identifies the bupleurum chinense and Tibetan bupleurum chinense, does not need sample pretreatment and has low cost.
The first aspect of the invention provides a method for identifying bupleurum chinense and bupleurum chinense, which comprises the following steps:
and (3) taking a radix bupleuri sample, incubating for 30-40min, and detecting by using headspace-gas chromatography-ion mobility spectrometry.
According to a specific embodiment of the invention, at least the following beneficial effects are achieved:
the identification method of bupleurum chinense and Tibetan bupleurum chinense in the invention utilizes the HS-GC-IMS technology to establish the volatile fingerprint of the HS-GC-IMS of bupleurum chinense and Tibetan bupleurum chinense so as to quickly identify bupleurum chinense and Tibetan bupleurum chinense. The identification method of bupleurum chinense and bupleurum chinense in the invention utilizes MXT-5 chromatographic column to detect and obtain 1 volatility characteristic peak of bupleurum chinense, 5 volatility characteristic peaks of bupleurum chinense, the total ratio of PC-1 and PC-2 in main component analysis is 87%, utilizes MXT-WAX chromatographic column to detect and obtain 4 volatility characteristic peaks of bupleurum chinense, 4 volatility characteristic peaks of bupleurum chinense and the total ratio of PC-1 and PC-2 in main component analysis is 91%. Thus, the MXT-WAX chromatographic column can better distinguish bupleurum chinense from Tibetan bupleurum chinense. The identification method is simple and quick, and provides reliable reference basis for detecting other traditional Chinese medicinal materials.
According to a first aspect of the invention, in some embodiments of the invention, the sample of Bupleurum falcatum is taken at a volume of 0.1 to 0.2 g.
According to the first aspect of the present invention, in some embodiments of the present invention, the incubation temperature of the Bupleurum falcatum sample is 110 ℃ to 120 ℃.
According to the first aspect of the present invention, in some embodiments of the present invention, the headspace-gas chromatography-ion mobility spectrometry is performed using an MXT-WAX column or an MXT-5 column having a column length of 10 to 15m, an inner diameter of 0.28 to 0.53mm, and a film thickness of 0.5 to 1.5. mu.m.
In some preferred embodiments of the present invention, the headspace-gas chromatography-ion mobility spectrometry is performed using an MXT-WAX column having a column length of 10 to 15m, an inner diameter of 0.28 to 0.53mm, and a film thickness of 0.5 to 1.5. mu.m.
In some more preferred embodiments of the invention, the MXT-WAX chromatography column has a column length of 15m, an inner diameter of 0.53mm and a membrane thickness of 1.0. mu.m.
According to a first aspect of the present invention, in some embodiments of the present invention, the MXT-WAX column or MXT-5 column has a column temperature of 75 to 80 ℃, an ion mobility spectrometry temperature of 75 to 80 ℃ and a needle temperature of 120 to 125 ℃.
In some preferred embodiments of the invention, the MXT-WAX column or MXT-5 column has a column temperature of 80 ℃, an ion mobility spectrometry temperature of 80 ℃ and a needle temperature of 125 ℃.
According to a first aspect of the invention, in some embodiments of the invention, the carrier/drift gas for headspace-gas chromatography-ion mobility spectrometry comprises nitrogen.
In some preferred embodiments of the present invention, the carrier/drift gas of the headspace-gas chromatography-ion mobility spectrometry is nitrogen.
In some preferred embodiments of the present invention, the carrier gas has a flow rate of:
the flow rate of the drift gas is 125-200 mL/min.
In some more preferred embodiments of the invention, the flow rate of the drift gas is 150 mL/min.
According to a first aspect of the invention, in some embodiments of the invention, the sample volume for headspace-gas chromatography-ion mobility spectrometry is 500 μ L.
In a second aspect of the invention, a detection device is provided, wherein the detection device comprises a headspace-gas chromatography-ion mobility spectrometry, an MXT-WAX chromatographic column or an MXT-5 chromatographic column.
In a third aspect of the present invention, there is provided the use of the identification method of the first aspect of the present invention in identification of bupleurum.
According to a third aspect of the invention, in some embodiments of the invention, the bupleurum comprises bupleurum chinense and bupleurum chinense.
In some preferred embodiments of the invention, the bupleurum falcatum and bupleurum tibetan are bupleurum chinense and bupleurum chinense.
In a fourth aspect of the invention, there is provided a use of the detection device according to the second aspect of the invention in bupleuri radix identification.
According to a fourth aspect of the invention, in some embodiments of the invention, the bupleurum comprises bupleurum chinense and bupleurum chinense.
In some preferred embodiments of the invention, the bupleurum falcatum and bupleurum falcatum are bupleurum falcatum.
Drawings
FIG. 1 shows the fingerprint of MXT-5 chromatographic columns of 21 batches of bupleurum chinense (A) and 17 batches of Tibetan bupleurum chinense (B) in the example of the present invention;
FIG. 2 is a Gallery spectrum of MXT-5 columns from 21 batches of bupleuri radix and 17 batches of Tibetan bupleuri radix in an embodiment of the present invention;
FIG. 3 is a diagram of the Int Dynamic PCA analysis of MXT-5 columns from 21 lots of bupleurum chinense and 17 lots of Tibetan bupleurum chinense in the present example;
FIG. 4 shows the fingerprint spectra of MXT-WAX chromatographic columns of 21 batches of bupleurum chinense (A) and 17 batches of Tibetan bupleurum chinense (B) in the example of the present invention;
FIG. 5 is a Gallery spectrum of MXT-WAX chromatography columns of 21 batches of bupleuri radix and 17 batches of Tibetan bupleuri radix in an embodiment of the present invention;
FIG. 6 is a diagram of the analysis of Int Dynamic PCA of MXT-WAX columns from 21 batches of bupleuri radix and 17 batches of Tibetan bupleuri radix in the example of the present invention;
FIG. 7 is a fingerprint of optimized sampling amounts of bupleurum chinense 10(A) and bupleurum chinense 14(B) in the embodiment of the present invention;
FIG. 8 is a fingerprint of the incubation temperature optimization of bupleurum chinense 10(A) and bupleurum chinense 14(B) in the embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and technical effects of the present invention more apparent, the present invention will be described in further detail with reference to specific embodiments. It should be understood that the detailed description and specific examples, while indicating the preferred embodiment of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.
The experimental materials and reagents used are, unless otherwise specified, all consumables and reagents which are conventionally available from commercial sources.
Experimental materials
(1) An experimental instrument:
headspace-gas chromatography-ion mobility spectrometer (g.a.s. company, germany) and associated analytical software lav (laboratory analytical viewer) and 3-part plug-in Reporter, GalleryPlot, Int Dynamic principles Components Analysis (Int Dynamic PCA).
(2) Experimental sample:
north radix bupleuri (number: BCH 1-21), Tibetan radix bupleuri (number: ZCH 1-17).
In the embodiment, the bupleurum chinense and the Tibetan bupleurum chinense are identified by the traditional Chinese medicine laboratory of the drug inspection institute in Guangdong province, and the varieties are determined without errors, wherein the detailed sources of the bupleurum chinense and the Tibetan bupleurum chinense are shown in table 1.
TABLE 1 sources of Bupleurum samples
All bupleurum samples need to be subpackaged before detection, and the method comprises the following specific steps:
taking 0.1-0.2 g of bupleurum sample to a 20mL headspace sample injection bottle, and incubating for 30-40min at 110-120 ℃.
Detection of Bupleurum samples
The incubated bupleurum samples in the above examples were injected through the headspace and subjected to separation detection using headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS), and each sample was measured 2 times in parallel.
The detection conditions of the HS-GC-IMS are as follows:
and (3) chromatographic column: MXT-WAX column (15 m.times.0.53 mm, 1.0 μm), MXT-5 column (15 m.times.0.53 mm, 1.0 μm). The carrier gas/drift gas is nitrogen, the carrier gas flow rate is 0-5 min, 5mL/min, 5-10 min, 5-25 mL/min, 10-15 min, 25-125 mL/min, 15-30 min, 125mL/min, and the drift gas flow rate is 150 mL/min. The column temperature is 80 ℃, the IMS temperature is 80 ℃ and the injection needle temperature is 125 ℃. The injection volume was 500. mu.L.
Detection result of Bupleurum sample
The method in the above example is adopted to detect 21 batches of bupleurum chinense and 17 batches of Tibetan bupleurum chinense, and the results are as follows.
(1) MXT-5 chromatographic column detection results:
MXT-5 chromatographic column is adopted to detect 21 batches of bupleuri radix and 17 batches of Tibetan bupleuri radix respectively, and typical spectra are shown in figure 1. Through statistics of typical spectra of bupleurum chinense and Tibetan bupleurum chinense, 1 volatility characteristic peak of bupleurum chinense and 5 volatility characteristic peaks of Tibetan bupleurum chinense are obtained. Gallery spectra of bupleuri radix and Tibetan bupleuri radix are shown in FIG. 2, wherein darker color indicates higher content of the volatile substances. As shown in FIG. 2, the main difference between Bupleurum scorzonerifolium and Bupleurum scorzonerifolium is the content of volatile characteristic substances. The main component analysis of 21 batches of bupleurum chinense and 17 batches of Tibetan bupleurum chinense is carried out by adopting Int Dynamic PCA, the result is shown in figure 3, 1-21 samples of bupleurum chinense are gathered together, 1-17 samples of Tibetan bupleurum chinense are gathered together, the two samples are independently gathered together, the obvious difference is shown, the total ratio of PC-1 to PC-2 is 87%, and the effective distinction of bupleurum chinense and Tibetan bupleurum chinense can be carried out by virtue of a low-polarity MXT-5 chromatographic column.
(2) And (3) detecting results of the MXT-WAX chromatographic column:
MXT-WAX chromatography column is adopted to detect 21 batches of bupleuri radix and 17 batches of Tibetan bupleuri radix respectively, and typical spectra are shown in figure 4. By counting typical spectrums of bupleurum chinense and Tibetan bupleurum chinense, 4 volatility characteristic peaks of bupleurum chinense and 4 volatility characteristic peaks of Tibetan bupleurum chinense are obtained. Gallery spectra of bupleuri radix and Tibetan bupleuri radix are shown in FIG. 5, wherein darker color indicates higher content of the volatile substances. As shown in FIG. 5, the main difference between Bupleurum scorzonerifolium and Bupleurum scorzonerifolium is the content of volatile characteristic substances. Main component analysis is carried out on 21 batches of bupleurum chinense and 17 Tibetan bupleurum chinense by adopting Int Dynamic PCA, the result is shown in figure 6, 1-21 samples of bupleurum chinense and 1-17 samples of Tibetan bupleurum chinense are respectively and independently gathered, the obvious difference is shown, the total proportion of PC-1 and PC-2 is 91%, and the strong polarity MXT-WAX chromatographic column can be used for effectively distinguishing the bupleurum chinense from the Tibetan bupleurum chinense.
Optimization of detection conditions
(1) Influence of sampling amount on detection results:
taking a sample of Bupleurum falcatum 10 and a sample of Bupleurum falcatum 14 respectively, weighing 0.05g, 0.1g, 0.2g and 0.3g respectively, incubating at 120 ℃ for 30min, and detecting by the method in the above example.
The result is shown in FIG. 7, the 0.05g of the samples of Bupleurum chinense and Bupleurum scorzonerifolium have less peak. The 0.2g and 0.3g bupleurum chinense and Tibetan bupleurum chinense samples have more peaks, but the signal intensity is too high, the peak separation degree is poor, and the identification is difficult. The bupleurum chinense and the Tibetan bupleurum chinense samples with the weight of 0.1-0.2 g have good peaks, and the separation degree between the peaks is good, so that the identification is easy. Therefore, the sampling amount of the selected bupleurum chinense and bupleurum chinense is 0.1-0.2 g.
(2) Influence of headspace incubation temperature on assay results:
taking bupleurum chinense No. 10 and Tibetan bupleurum chinense No. 14 as samples, adopting the detection method, taking samples of 0.1-0.2 g, fixing incubation time for 30min, and respectively incubating at 80 ℃, 90 ℃, 100 ℃, 110 ℃ and 120 ℃.
As shown in FIG. 8, the number of peaks appeared in the bupleuri radix sample with the increase of incubation temperature, but the headspace incubation temperature was selected to be 110 ℃ to 120 ℃ in consideration of the possibility of thermal decomposition of volatile substances and decrease of analysis sensitivity due to the excessively high temperature.
(3) Effect of headspace incubation time on assay results:
taking bupleurum chinense No. 10 and bupleurum chinense No. 14 as samples, adopting the detection method, taking samples of 0.1-0.2 g, and incubating at a fixed incubation temperature of 120 ℃ for 10min, 20min, 30min, 40min and 50min respectively.
The result shows that the number of peaks appeared in the bupleurum sample is increased along with the increase of the incubation time, but the number and the intensity of the peaks appeared in the bupleurum sample after the bupleurum sample is incubated for 30-40min are not obviously increased, and considering that volatile substances with unstable properties in the bupleurum sample can be degraded, oxidized and the like along with the extension of the heating time, the headspace incubation time is selected to be 30-40 min.
Comparison of detection methods
The HS-GC-IMS is a technology for combining headspace gas chromatography and ion mobility spectrometry, so that the HS-GC-IMS not only has the characteristic that the headspace gas chromatography does not need sample pretreatment and prominent separation, but also has the advantages of high sensitivity, high resolution, simple operation, portability and the like of the ion mobility spectrometry. The HS-GC-IMS can be used for quickly detecting volatile substances in a sample to be detected from a molecular level, and can be applied to the detection fields of traditional Chinese medicines, cosmetics, foods and the like.
In the related technology, a static headspace-capillary gas chromatography-mass spectrometry, an ultra-high performance liquid chromatography and tandem quadrupole time-of-flight mass spectrometer combined technology (UPLC-Q-TOF-MS) all need to relate to mass spectrometry detection, the detection cost is high, the UPLC-Q-TOF-MS is not suitable for large-scale popularization and use, a complex sample pretreatment process is needed, and the detection difficulty is high. The HS-GC-IMS in the embodiment of the invention can quickly identify the bupleurum chinense and the Tibetan bupleurum chinense, and the method does not need sample pretreatment, has short analysis period, low detection cost and portable instrument.
In conclusion, the HS-GC-IMS detection method in the embodiment of the invention can simply and rapidly identify bupleurum chinense and Tibetan bupleurum chinense, and obtain the volatility characteristic peaks (4 in each case) of bupleurum chinense and Tibetan bupleurum chinense, thereby providing reliable reference data for identifying bupleurum chinense and Tibetan bupleurum chinense.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such modifications are intended to be included in the scope of the present invention.
Claims (2)
1. A method for identifying bupleurum chinense and Tibetan bupleurum chinense comprises the following steps:
incubating a radix bupleuri sample for 30-40min at the incubation temperature of 110-120 ℃, and detecting by using headspace-gas chromatography-ion mobility spectrometry;
wherein the headspace-gas chromatography-ion mobility spectrometry is detected by using an MXT-WAX chromatographic column or an MXT-5 chromatographic column, the column length of the MXT-WAX chromatographic column or the MXT-5 chromatographic column is 10-15 m, the inner diameter of the MXT-WAX chromatographic column or the MXT-5 chromatographic column is 0.28-0.53 mm, the film thickness of the MXT-WAX chromatographic column or the MXT-5 chromatographic column is 0.5-1.5 mu m, the column temperature of the MXT-WAX chromatographic column or the MXT-5 chromatographic column is 75-80 ℃, the ion mobility spectrometry temperature of the MXT-WAX chromatographic column or the MXT-5 chromatographic column is 75-80 ℃, and the injection needle temperature of the MXT-WAX chromatographic column is 120-125 ℃;
the carrier/drift gas of the headspace-gas chromatography-ion mobility spectrometry comprises nitrogen;
the flow rate of the carrier gas is as follows:
the flow rate of the drift gas is 125-200 mL/min.
2. The identification method according to claim 1, wherein the sample amount of the Bupleurum chinense sample is 0.1-0.2 g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110204917.3A CN112782320B (en) | 2021-02-24 | 2021-02-24 | Method for identifying bupleurum chinense and Tibetan bupleurum chinense and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110204917.3A CN112782320B (en) | 2021-02-24 | 2021-02-24 | Method for identifying bupleurum chinense and Tibetan bupleurum chinense and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112782320A CN112782320A (en) | 2021-05-11 |
| CN112782320B true CN112782320B (en) | 2022-07-22 |
Family
ID=75761769
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110204917.3A Active CN112782320B (en) | 2021-02-24 | 2021-02-24 | Method for identifying bupleurum chinense and Tibetan bupleurum chinense and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112782320B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115219620B (en) * | 2022-07-14 | 2024-01-26 | 西北大学 | Jingyang Fu tea specific identification volatile component combination, preparation method and application thereof, and Jingyang Fu tea identification method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011157781A1 (en) * | 2010-06-17 | 2011-12-22 | Step Sensortechnik Und Elektronik Pockau Gmbh | Method for ion mobility spectrometry |
| CN106483187A (en) * | 2016-10-11 | 2017-03-08 | 王海燕 | Chinese medicine based on ion mobility spectrometry and similarity easily mixes adulterant discrimination method with which |
| CN109142590A (en) * | 2018-07-11 | 2019-01-04 | 广州市药品检验所 | The identification classification method and device of citrus chachiensis hortorum |
| CN109633058A (en) * | 2019-01-24 | 2019-04-16 | 蕲春县产品质量检验检测中心 | A kind of wormwood place of production discriminating classification method and system |
-
2021
- 2021-02-24 CN CN202110204917.3A patent/CN112782320B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011157781A1 (en) * | 2010-06-17 | 2011-12-22 | Step Sensortechnik Und Elektronik Pockau Gmbh | Method for ion mobility spectrometry |
| CN106483187A (en) * | 2016-10-11 | 2017-03-08 | 王海燕 | Chinese medicine based on ion mobility spectrometry and similarity easily mixes adulterant discrimination method with which |
| CN109142590A (en) * | 2018-07-11 | 2019-01-04 | 广州市药品检验所 | The identification classification method and device of citrus chachiensis hortorum |
| CN109633058A (en) * | 2019-01-24 | 2019-04-16 | 蕲春县产品质量检验检测中心 | A kind of wormwood place of production discriminating classification method and system |
Non-Patent Citations (2)
| Title |
|---|
| 中药柴胡与常见混伪品的鉴别方法分析;孙旖;《光明中医》;20180930;第33卷(第17期);第2502-2504页 * |
| 气相色谱-离子迁移谱应用于橄榄油的掺假鉴别;李淑静 等;《食品研究与开发》;20180831(第15期);第109-116页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112782320A (en) | 2021-05-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Yan et al. | An effective method for determining the ingredients of S huanghuanglian formula in blood samples using high‐resolution LC–MS coupled with background subtraction and a multiple data processing approach | |
| WO2014067478A1 (en) | Method for establishing shenqi fuzheng injection fingerprint spectrum | |
| Luo et al. | Quality evaluation of Salvia miltiorrhiza Bge. by ultra high performance liquid chromatography with photodiode array detection and chemical fingerprinting coupled with chemometric analysis | |
| Dou et al. | Quality evaluation of rhubarb based on qualitative analysis of the HPLC fingerprint and UFLC–Q‐TOF–MS/MS combined with quantitative analysis of eight anthraquinone glycosides by QAMS | |
| Niu et al. | Quantitative analysis and chromatographic fingerprinting of the semen zizyphi spinosae by ultra‐high‐performance liquid chromatography coupled with diode‐array detector | |
| Guo et al. | Rapid analysis of Corni fructus using paper spray‐mass spectrometry | |
| Wang et al. | Simultaneous determination of nucleosides, myriocin, and carbohydrates in Cordyceps by HPLC coupled with diode array detection and evaporative light scattering detection | |
| CN108896681B (en) | Nerve-soothing brain-tonifying liquid multi-index quantitative fingerprint establishment method and application thereof | |
| Zhang et al. | Application of ultrahigh‐performance liquid chromatography coupled with mass spectrometry for analysis of lignans and quality control of Fructus Schisandrae chinensis | |
| CN112782320B (en) | Method for identifying bupleurum chinense and Tibetan bupleurum chinense and application thereof | |
| CN113049720A (en) | Method for detecting residual quantity of ethanol in compound fresh bamboo juice | |
| Dai et al. | Simultaneous chemical fingerprint and quantitative analysis of Rhizoma Smilacis Glabrae by accelerated solvent extraction and high‐performance liquid chromatography with tandem mass spectrometry | |
| Qi et al. | Application of high-performance liquid chromatography–electrospray ionization time-of-flight mass spectrometry for analysis and quality control of Radix Astragali and its preparations | |
| Duan et al. | Chemical fingerprint analysis of Fritillaria delavayi Franch. by high‐performance liquid chromatography | |
| Du et al. | Simultaneous qualitative and quantitative analysis of 28 components in Isodon rubescens by HPLC‐ESI‐MS/MS | |
| Liu et al. | Quantitative and fingerprinting analysis of Atractylodes rhizome based on gas chromatography with flame ionization detection combined with chemometrics | |
| Deng et al. | Simultaneous LC–MS quantification of 15 lignans in Schisandra chinensis (Turcz.) Baill. fruit | |
| Deng et al. | A validated LC‐MS/MS method for rapid determination of brazilin in rat plasma and its application to a pharmacokinetic study | |
| Zhang et al. | Development and validation of HPLC coupled with triple quadrupole MS for the simultaneous determination of six phenolic acids, six flavonoids, and a lignan in Polygonum capitatum | |
| Zhou et al. | Chemical profiling and quantification of multiple components in Jin‐Gu‐Lian capsule using a multivariate data processing approach based on UHPLC‐Orbitrap Exploris 240 MS and UHPLC‐MS/MS | |
| Liu et al. | Combination of the advantages of chromatographic methods based on active components for the quality evaluation of licorice | |
| Chen et al. | Quantitative fingerprinting based on the limited‐ratio quantified fingerprint method for an overall quality consistency assessment and antioxidant activity determination of Lianqiao Baidu pills using HPLC with a diode array detector combined with chemometric methods | |
| Zhang et al. | Comprehensive quality evaluation of shuxuening injection employing quantitative high-performance liquid chromatography fingerprint and chemometrics | |
| Yang et al. | Comparison of pharmacokinetics of phytoecdysones and triterpenoid saponins of monomer, crude and processed Radix Achyranthis Bidentatae by UHPLC-MS/MS | |
| Woźniakiewicz et al. | Development of the MAE/UHPLC-MS-TOF method for determination of benzodiazepines in human bio-fluids for toxicological analysis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |