CN112778991A - Biological enzyme composite blocking remover and preparation method thereof - Google Patents
Biological enzyme composite blocking remover and preparation method thereof Download PDFInfo
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- CN112778991A CN112778991A CN202110032402.XA CN202110032402A CN112778991A CN 112778991 A CN112778991 A CN 112778991A CN 202110032402 A CN202110032402 A CN 202110032402A CN 112778991 A CN112778991 A CN 112778991A
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Abstract
The invention relates to a biological enzyme composite blocking remover and a preparation method thereof, belonging to the technical field of oilfield production increase modification, wherein the blocking remover comprises the following components in percentage by mass: 0.1-0.5% of biological enzyme, 3-5% of biological surfactant, 5-10% of biological enzyme stabilizer and the balance of water; the biological enzyme comprises at least one of the following: xylanase, lipase, cellulase, mannase, protein complex enzyme, amylase and xanthan gum lyase; the biological surfactant comprises at least one of the following components: the biological surfactant mainly comprises rhamnolipid, sophorolipid and N-acetylgalactosamine; the biological enzyme stabilizer comprises at least one of the following components: disodium ethylene diamine tetraacetate, citric acid and triethanolamine; the biological enzyme composite blocking remover provided by the invention can solve the problems of residual gum in low-permeability and medium-low permeability reservoir modification, reduced permeability caused by organic matter (wax, asphaltene and the like) blocking in the later development period and the like.
Description
Technical Field
The invention belongs to the technical field of yield increase modification of oil fields, and particularly relates to a biological enzyme composite blocking remover and a preparation method thereof.
Background
The biological enzyme is an inactive catalyst using protein as matrix, is developed by adopting modern biochemical techniques of modern gene engineering, cell engineering, enzyme engineering and the like, and has the advantages of rapidness, high efficiency, environmental protection and the like. With the development of oilfield chemical technology, the combination degree of oilfield chemical agents and environment-friendly biological agents is continuously improved, and the biological enzymatic plugging agent with obvious environmental protection characteristics and high efficiency is applied to plugging removal and modification of oil-water wells of various oil fields in China, such as oil fields of Daqing, Shengli and the like, due to multiple advantages, and achieves good effects.
In 2019, the invention provides a biological enzymatic plugging agent product suitable for oil and gas fields, which mainly comprises mannase, cellulase, xanthan gum lyase and lipase, wherein the biological enzymatic plugging agent needs a pH regulator for use, and the pH value application range is limited. In 2017, a biological enzymatic hydrolysis plugging yield-increasing agent is reported from hibiscus makinoi and the like, and the biological agent consists of xylanase, cellulase, rhamnolipid and the like, so that the biological agent is good in environmental protection, meets the HSE standard of an oil field, has no pollution to the production environment and has remarkable oil dirt cleaning capacity; the water-soluble product has good moistening effect, can accelerate the movement of water molecules and improve the movement capability of oil and water; the capability of dredging oil flow channels is good. The biological enzymolysis plugging agent has insufficient temperature resistance. The product consists of protein-complex enzyme, composite biological active substances, hemicellulase, a biological active agent and water, can effectively remove colloid, asphaltene and wax, can prevent scale deposition and has a good improvement effect on a reservoir core; meanwhile, the field application process of the biological enzymatic plugging agent is provided. The biological enzymolysis plugging agent has insufficient temperature resistance. The invention relates to a biological enzyme-hydrolyzed plugging agent for stratum plugging removal in the field of oil and gas field exploitation, which can be used for removing plugs of oil wells, water injection wells, polymer injection wells and gas wells, and can be injected into oil and gas wells after being diluted with water according to a certain mass ratio. The biological enzyme-hydrolyzed plugging agent has short shelf life and does not show temperature resistance.
Therefore, a novel high-efficiency biological enzyme composite blocking remover is needed, the technical problems of permeability reduction, capacity reduction and the like caused by low-permeability and medium-low-permeability reservoir modification residual gum and organic matter (wax, asphaltene and the like) blocking in the later development period can be solved, and technical support is provided for efficient development of low-efficiency wells.
Disclosure of Invention
In view of the above problems, the present invention has been made to provide a complex biological enzyme deblocking agent that overcomes or at least partially solves the above problems, and a method for preparing the same.
The embodiment of the invention provides a biological enzyme composite blocking remover, which comprises the following components in percentage by mass:
0.1-0.5% of biological enzyme, 3-5% of biological surfactant, 5-10% of biological enzyme stabilizer and the balance of water;
the biological enzyme comprises at least one of the following:
xylanase, lipase, cellulase, mannase, protein complex enzyme, amylase and xanthan gum lyase;
the biological surfactant comprises at least one of the following components:
the biological surfactant mainly comprises rhamnolipid, sophorolipid and N-acetylgalactosamine;
the biological enzyme stabilizer comprises at least one of the following components:
disodium ethylene diamine tetraacetate, citric acid and triethanolamine.
Optionally, the blocking remover comprises the following components in percentage by mass:
0.2% of biological enzyme, 3% of biological surfactant, 6% of biological enzyme stabilizer and the balance of water;
the components of the biological enzyme are xylanase, cellulase, mannase, amylase and xanthan gum lyase;
the components of the biological surfactant are rhamnolipid, sophorolipid and N-acetylgalactosamine;
the components of the biological enzyme stabilizer are disodium ethylene diamine tetraacetate, citric acid and triethanolamine.
Optionally, the mass ratio of the xylanase, the cellulase, the mannanase, the amylase and the xanthan gum lyase is 4: 2: 3: 1;
the mass ratio of the rhamnolipid, the sophorolipid and the N-acetylgalactosamine is 2: 1;
the mass ratio of the disodium ethylene diamine tetraacetate, the citric acid and the triethanolamine is 20: 5: 3.
Optionally, the blocking remover comprises the following components in percentage by mass:
0.3% of biological enzyme, 3% of biological surfactant, 8% of biological enzyme stabilizer and the balance of water;
the biological enzyme comprises xylanase, lipase, mannase, protein complex enzyme and amylase;
the components of the biological surfactant are rhamnolipid, sophorolipid and N-acetylgalactosamine;
the components of the biological enzyme stabilizer are disodium ethylene diamine tetraacetate, citric acid and triethanolamine.
Optionally, the mass ratio of the xylanase, the lipase, the mannase, the protein complex enzyme and the amylase is 3: 2: 1;
the mass ratio of the rhamnolipid, the sophorolipid and the N-acetylgalactosamine is 2: 1;
the mass ratio of the disodium ethylene diamine tetraacetate, the citric acid and the triethanolamine is 15: 10: 3.
Optionally, the blocking remover comprises the following components in percentage by mass:
0.3% of biological enzyme, 5% of biological surfactant, 8% of biological enzyme stabilizer and the balance of water;
the biological enzyme comprises xylanase, mannase, protein complex enzyme and xanthan gum lyase;
the components of the biological surfactant are rhamnolipid and N-acetylgalactosamine;
the components of the biological enzyme stabilizer are disodium ethylene diamine tetraacetate, citric acid and triethanolamine.
Optionally, the mass ratio of the xylanase, the mannase, the protein complex enzyme and the xanthan gum lyase is 10: 20: 2: 5;
the mass ratio of the rhamnolipid to the N-acetylgalactosamine is 1: 1;
the mass ratio of the disodium ethylene diamine tetraacetate, the citric acid and the triethanolamine is 5: 1.
Optionally, the blocking remover comprises the following components in percentage by mass:
0.3% of biological enzyme, 4% of biological surfactant, 6% of biological enzyme stabilizer and the balance of water;
the components of the biological enzyme are lipase, cellulase, mannase, protein complex enzyme and amylase;
the components of the biological surfactant are rhamnolipid, sophorolipid and N-acetylgalactosamine;
the components of the biological enzyme stabilizer are disodium ethylene diamine tetraacetate, citric acid and triethanolamine.
Optionally, the mass ratio of the lipase, the cellulase, the mannase, the protein complex enzyme and the amylase is 10: 20: 10: 3;
the mass ratio of the rhamnolipid, the sophorolipid and the N-acetylgalactosamine is 2: 1: 4;
the mass ratio of the disodium ethylene diamine tetraacetate, the citric acid and the triethanolamine is 5: 10: 1.
Based on the same inventive concept, the embodiment of the invention also provides a preparation method of the biological enzyme composite blocking remover, which is used for preparing the biological enzyme composite blocking remover, and the method comprises the following steps:
obtaining the components in parts by mass, wherein the components comprise 0.1-0.5% of biological enzyme, 3-5% of biological surfactant, 5-10% of biological enzyme stabilizer and the balance of water;
and sequentially adding the biological surfactant, the biological enzyme stabilizer and the biological enzyme into the water, and uniformly mixing to obtain the biological enzyme composite blocking remover.
One or more technical solutions in the embodiments of the present invention have at least the following technical effects or advantages:
the embodiment of the invention provides a biological enzyme composite blocking remover, which comprises the following components in percentage by mass: 0.1-0.5% of biological enzyme, 3-5% of biological surfactant, 5-10% of biological enzyme stabilizer and the balance of water; the biological enzyme comprises at least one of the following: xylanase, lipase, cellulase, mannase, protein complex enzyme, amylase and xanthan gum lyase; the biological surfactant comprises at least one of the following components: the biological surfactant mainly comprises rhamnolipid, sophorolipid and N-acetylgalactosamine; the biological enzyme stabilizer comprises at least one of the following components: disodium ethylene diamine tetraacetate, citric acid and triethanolamine. The biological enzyme can effectively promote the degradation of glycosidic bonds in the vegetable gum, so that blockage can be relieved, the biological surfactant can effectively reduce the surface interfacial tension of the blocking remover, the blocking remover is prevented from being adsorbed in the stratum, the blocking removal effect is reduced, the biological enzyme stabilizer can keep the activity of the biological enzyme, and the failure of the biological enzyme is avoided.
The foregoing description is only an overview of the technical solutions of the present invention, and the embodiments of the present invention are described below in order to make the technical means of the present invention more clearly understood and to make the above and other objects, features, and advantages of the present invention more clearly understandable.
Detailed Description
The present invention will be described in detail below with reference to specific embodiments and examples, and the advantages and various effects of the present invention will be more clearly apparent therefrom. It will be understood by those skilled in the art that these specific embodiments and examples are for the purpose of illustrating the invention and are not to be construed as limiting the invention.
Throughout the specification, unless otherwise specifically noted, terms used herein should be understood as having meanings as commonly used in the art. Accordingly, unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. If there is a conflict, the present specification will control.
Unless otherwise specifically stated, various raw materials, reagents, instruments, equipment and the like used in the present invention are commercially available or can be prepared by existing methods.
In order to solve the technical problems, the general idea of the embodiment of the application is as follows:
according to a typical embodiment of the invention, a biological enzyme composite blocking remover is provided, the blocking remover can remove the blocking of polysaccharide vegetable gum, and the blocking remover comprises the following components in percentage by mass:
0.1-0.5% of biological enzyme, 3-5% of biological surfactant, 5-10% of biological enzyme stabilizer and the balance of water;
the biological enzyme comprises at least one of the following:
xylanase, lipase, cellulase, mannase, protein complex enzyme, amylase and xanthan gum lyase;
the biological surfactant comprises at least one of the following components:
the biological surfactant mainly comprises rhamnolipid, sophorolipid and N-acetylgalactosamine;
the biological enzyme stabilizer comprises at least one of the following components:
disodium ethylene diamine tetraacetate, citric acid and triethanolamine.
The biological enzyme has the effect of degrading the plant gum glycosidic bond, the reason for controlling the mass fraction of the biological enzyme to be 0.1-0.5% is to reduce the comprehensive cost while ensuring the performance index, the adverse effect of overlarge mass fraction value is that the comprehensive cost is too high, and the adverse effect of undersize is that the glycosidic bond degradation cannot be realized;
the biological surfactant has the effects of reducing the surface interfacial tension of the blocking remover, the reason for controlling the mass fraction of the biological surfactant to be 3-5% is to reduce the comprehensive cost while ensuring the performance index, the adverse effect of overlarge mass fraction is that the comprehensive cost is too high, and the adverse effect of undersize is that the surface interfacial tension is too large, so that the blocking removal effect is reduced;
the biological enzyme stabilizer has the function of keeping the activity of biological enzyme, the reason for controlling the mass fraction of the biological enzyme stabilizer to be 5-10% is to reduce the comprehensive cost while ensuring the performance index, the adverse effect of overlarge mass fraction is that the comprehensive cost is overhigh, and the adverse effect of undersize is that the biological enzyme is unstable and easy to lose efficacy;
xylanase, property: is a heterogeneous polysaccharide with high degree of branching and complex structure, and contains a plurality of different substituents. The product has wide distribution in nature, can be obtained from animals, plants and microorganisms, and has wide application in brewing, feed and other industries.
Lipase, property: belonging to the class of carboxyl ester hydrolases and are capable of progressively hydrolyzing triglycerides into glycerol and fatty acids. It is present in animal, plant and microbial tissues containing fat.
Cellulase, property: is a complex enzyme, mainly comprises exo-beta-glucanase, endo-beta-glucanase, beta-glucosidase and the like, and also has xylanase with high activity. Acting on cellulose and products derived from cellulose.
Mannanase, properties: a multifunctional growth promoter has effects in promoting secretion of insulin-like growth factor IGF-I, and promoting protein synthesis.
Protein complex enzyme, property: they are capable of hydrolyzing protein peptide chains, which can be divided into two classes, endopeptidases and exopeptidases, according to the way they hydrolyze polypeptides. Endopeptidases cleave the protein molecule internally, forming smaller molecular weight peptides and peptones. Exopeptidases hydrolyze peptide bonds one by one from the free amino or carboxyl terminus of the protein molecule to free amino acids, aminopeptidases and carboxypeptidases.
Amylase, property: enzymes for hydrolyzing starch and glycogen are generally called as enzymes for catalyzing and hydrolyzing starch slurry on fabrics by amylase, and due to the high efficiency and specificity of the amylase, the desizing rate of enzyme desizing is high, desizing is fast, pollution is less, and products are softer than products prepared by an acid method and an alkaline method and do not damage fibers.
Xanthan lyase, property: can effectively degrade xanthan gum and generate oligosaccharide micro-bacteria, and degrade xanthan gum molecules.
Rhamnolipid, nature: a biosurfactant of a biological metabolic nature produced by Pseudomonas or Burkholderia species. The anionic surfactant exists naturally in soil, water and plant and belongs to glycolipid.
Sophorolipid, nature: the candida is a microbial secondary metabolite produced by taking sugar, vegetable oil and the like as carbon sources through a fermentation process under certain conditions, has the general performances of solubilization, emulsification, wetting, foaming, dispersion, surface tension reduction and the like of a conventional surfactant, and has the characteristics of no toxicity, 100% biodegradability, temperature resistance, high salt resistance, wide pH range adaptation, environmental friendliness and the like.
N-acetylgalactosamine, property: the hydroxyl on C-2 position of galactose is replaced by amino, and then the derivative is obtained after acetylation.
Disodium ethylene diamine tetraacetate, property: tasteless and odorless or slightly salty white or milky white crystalline or granular powder, and odorless and tasteless. It is water soluble and very insoluble in ethanol. It is an important chelating agent that chelates metal ions in solution. Prevent discoloration, deterioration, turbidity and oxidation loss of vitamin C caused by metal, and improve oxidation resistance of oil.
Citric acid, property: an important organic acid, colorless crystal, often contains one molecule of crystal water, is odorless, has strong sour taste, and is easily soluble in water. The anhydrous citric acid can be obtained by controlling the proper temperature during the crystallization. Has a great number of applications in industry, food industry, cosmetics industry and the like.
Triethanolamine, property: an organic compound is a colorless to pale yellow transparent viscous liquid, has slight ammonia smell, and becomes colorless to pale yellow cubic crystal system crystals at low temperature. When exposed to air, the color gradually darkens. Is easily soluble in water, ethanol, acetone, glycerol, ethylene glycol, etc., is slightly soluble in benzene, diethyl ether, carbon tetrachloride, etc., and is hardly soluble in nonpolar solvents.
The details of the bio-enzyme complex blocking remover and the preparation method thereof will be described below with reference to examples, comparative examples and experimental data.
Example 1
The preparation method of the biological enzyme composite blocking remover provided by the embodiment comprises the following steps:
s1, preparing the biological surfactant. In a beaker, rhamnolipid, sophorolipid and N-acetylgalactosamine are added according to the mass ratio of 2: 1 and are stirred uniformly.
S2, preparing the biological enzyme stabilizer. Adding disodium ethylene diamine tetraacetate, citric acid and triethanolamine into a beaker according to the mass ratio of 2: 0.5: 0.3, and uniformly stirring.
S3, preparing the biological enzyme. Adding xylanase, cellulase, mannase, amylase and xanthan gum lyase into a beaker according to the mass ratio of 4: 2: 3: 1, and uniformly stirring.
S4, adding 90.8 parts of water into a beaker, then sequentially adding 3 parts of biological surfactant, 6 parts of biological enzyme stabilizer and 0.2 part of biological enzyme, and uniformly stirring to obtain the biological enzyme composite blocking remover.
Example 2
The preparation method of the biological enzyme composite blocking remover provided by the embodiment comprises the following steps:
s1, preparing the biological surfactant. In a beaker, rhamnolipid, sophorolipid and N-acetylgalactosamine are added according to the mass ratio of 2: 1 and are stirred uniformly.
S2, preparing the biological enzyme stabilizer. In a beaker, the ratio of 1.5: 1: adding disodium ethylene diamine tetraacetate, citric acid and triethanolamine in a mass ratio of 0.3, and uniformly stirring.
S3, preparing the biological enzyme. Adding xylanase, lipase, mannase, protein complex enzyme and amylase into a beaker according to the mass ratio of 3: 2: 1, and uniformly stirring.
S4, adding 88.7 parts of water into a beaker, then sequentially adding 3 parts of biological surfactant, 8 parts of biological enzyme stabilizer and 0.3 part of biological enzyme, and uniformly stirring to obtain the biological enzyme composite blocking remover.
Example 3
The preparation method of the biological enzyme composite blocking remover provided by the embodiment comprises the following steps:
s1, preparing the biological surfactant. Adding rhamnolipid and N-acetylgalactosamine in a beaker according to the mass ratio of 1: 1, and uniformly stirring.
S2, preparing the biological enzyme stabilizer. Adding disodium ethylene diamine tetraacetate, citric acid and triethanolamine into a beaker according to the mass ratio of 1: 0.2, and uniformly stirring.
S3, preparing the biological enzyme. Adding xylanase, mannase, protein complex enzyme and xanthan gum lyase into a beaker according to the mass ratio of 1: 2: 0.2: 0.5, and uniformly stirring.
S4, adding 86.7 parts of water into a beaker, then sequentially adding 5 parts of biological surfactant, 8 parts of biological enzyme stabilizer and 0.3 part of biological enzyme, and uniformly stirring to obtain the biological enzyme composite blocking remover.
Example 4
The preparation method of the biological enzyme composite blocking remover provided by the embodiment
S1, preparing the biological surfactant. Adding rhamnolipid, sophorolipid and N-acetylgalactosamine into a beaker according to the mass ratio of 1: 0.5: 2, and uniformly stirring.
S2, preparing the biological enzyme stabilizer. Adding disodium ethylene diamine tetraacetate, citric acid and triethanolamine into a beaker according to the mass ratio of 1: 2: 0.2, and uniformly stirring.
S3, preparing the biological enzyme. Adding lipase, cellulase, mannase, protein complex enzyme and amylase into a beaker according to the mass ratio of 1: 2: 1: 0.3, and uniformly stirring.
And S4, adding 89.7 parts of water into a beaker, then sequentially adding 4 parts of biological surfactant, 6 parts of biological enzyme stabilizer and 0.3 part of biological enzyme, and uniformly stirring to obtain the biological enzyme composite blocking remover.
Comparative example 1
This example is different from example 1 in that it does not contain a biosurfactant.
Comparative example 2
This example is different from example 1 in that no bio-enzyme stabilizer is included.
Related experiments:
the performance of the biological enzyme composite blocking remover prepared in the examples 1-4 and the comparative examples 1-2 is tested, and the test results are shown in the table 1.
The related test method comprises the following steps:
(1) temperature resistance: and (3) heating the biological enzymatic plugging agent to a specified temperature, standing for 12 hours, and taking out to perform an evaluation experiment.
(2) And adding a small amount of acid-base solution into the biological enzymatic plugging agent, adjusting the pH value to a specified value, standing for 12 hours, and then performing a performance evaluation experiment.
(3) And (3) residual gum deblocking rate, preparing a certain amount of vegetable gum residual gum, adding a biological enzymolysis blocking agent into the residual gum, recording the degradation time of the residual gum, recording the mass of the residual gum after 12 hours if the residual gum is not completely degraded, and calculating the deblocking rate.
(4) Adding a biological enzymolysis blocking agent into the organic matters, and calculating the blockage removal rate according to the mass of the organic matters before and after reaction.
(5) And (3) placing the biological enzymatic plugging agent at 0 ℃, taking out the biological enzymatic plugging agent periodically, and performing performance evaluation.
(6) And (3) placing the biological enzymatic plugging agent at room temperature (20-25 ℃), taking out the biological enzymatic plugging agent regularly, and performing performance evaluation.
TABLE 1
As can be seen from Table 1, the biological enzyme composite blocking remover prepared by the embodiment of the invention can solve the problem of permeability reduction caused by the blocking of low-permeability and medium-low permeability reservoir transformation residual gum and organic matters (wax, asphaltene and the like) in the later development period, the temperature resistance is more than or equal to 150 ℃, the pH value adaptation range is 4.0-10.0, the residual gum blocking removal rate is more than or equal to 90.0%, the organic matter blocking removal rate is more than or equal to 90.0%, the quality guarantee period is more than two years at 0 ℃, and the quality guarantee period is more than or equal to 60d at room temperature.
Finally, it should also be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. Therefore, it is intended that the appended claims be interpreted as including preferred embodiments and all such alterations and modifications as fall within the scope of the invention.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (10)
1. The biological enzyme composite blocking remover is characterized by comprising the following components in parts by mass:
0.1-0.5% of biological enzyme, 3-5% of biological surfactant, 5-10% of biological enzyme stabilizer and the balance of water;
the biological enzyme comprises at least one of the following:
xylanase, lipase, cellulase, mannase, protein complex enzyme, amylase and xanthan gum lyase;
the biological surfactant comprises at least one of the following components:
the biological surfactant mainly comprises rhamnolipid, sophorolipid and N-acetylgalactosamine;
the biological enzyme stabilizer comprises at least one of the following components:
disodium ethylene diamine tetraacetate, citric acid and triethanolamine.
2. The biological enzyme composite blocking remover according to claim 1, wherein the blocking remover comprises the following components in percentage by mass:
0.2% of biological enzyme, 3% of biological surfactant, 6% of biological enzyme stabilizer and the balance of water;
the components of the biological enzyme are xylanase, cellulase, mannase, amylase and xanthan gum lyase;
the components of the biological surfactant are rhamnolipid, sophorolipid and N-acetylgalactosamine;
the components of the biological enzyme stabilizer are disodium ethylene diamine tetraacetate, citric acid and triethanolamine.
3. The biological enzyme composite blocking remover according to claim 2, wherein the mass ratio of the xylanase, the cellulase, the mannanase, the amylase and the xanthan gum lyase is 4: 2: 3: 1;
the mass ratio of the rhamnolipid, the sophorolipid and the N-acetylgalactosamine is 2: 1;
the mass ratio of the disodium ethylene diamine tetraacetate, the citric acid and the triethanolamine is 20: 5: 3.
4. The biological enzyme composite blocking remover according to claim 1, wherein the blocking remover comprises the following components in percentage by mass:
0.3% of biological enzyme, 3% of biological surfactant, 8% of biological enzyme stabilizer and the balance of water;
the biological enzyme comprises xylanase, lipase, mannase, protein complex enzyme and amylase;
the components of the biological surfactant are rhamnolipid, sophorolipid and N-acetylgalactosamine;
the components of the biological enzyme stabilizer are disodium ethylene diamine tetraacetate, citric acid and triethanolamine.
5. The biological enzyme composite blocking remover according to claim 4, wherein the mass ratio of the xylanase, the lipase, the mannase, the protein composite enzyme and the amylase is 3: 2: 1;
the mass ratio of the rhamnolipid, the sophorolipid and the N-acetylgalactosamine is 2: 1;
the mass ratio of the disodium ethylene diamine tetraacetate, the citric acid and the triethanolamine is 15: 10: 3.
6. The biological enzyme composite blocking remover according to claim 1, wherein the blocking remover comprises the following components in percentage by mass:
0.3% of biological enzyme, 5% of biological surfactant, 8% of biological enzyme stabilizer and the balance of water;
the biological enzyme comprises xylanase, mannase, protein complex enzyme and xanthan gum lyase;
the components of the biological surfactant are rhamnolipid and N-acetylgalactosamine;
the components of the biological enzyme stabilizer are disodium ethylene diamine tetraacetate, citric acid and triethanolamine.
7. The biological enzyme composite blocking remover according to claim 6, wherein the mass ratio of the xylanase, the mannase, the protein composite enzyme and the xanthan gum lyase is 10: 20: 2: 5;
the mass ratio of the rhamnolipid to the N-acetylgalactosamine is 1: 1;
the mass ratio of the disodium ethylene diamine tetraacetate, the citric acid and the triethanolamine is 5: 1.
8. The biological enzyme composite blocking remover according to claim 1, wherein the blocking remover comprises the following components in percentage by mass:
0.3% of biological enzyme, 4% of biological surfactant, 6% of biological enzyme stabilizer and the balance of water;
the components of the biological enzyme are lipase, cellulase, mannase, protein complex enzyme and amylase;
the components of the biological surfactant are rhamnolipid, sophorolipid and N-acetylgalactosamine;
the components of the biological enzyme stabilizer are disodium ethylene diamine tetraacetate, citric acid and triethanolamine.
9. The biological enzyme composite blocking remover according to claim 8, wherein the mass ratio of the lipase, the cellulase, the mannanase, the protein composite enzyme and the amylase is 10: 20: 10: 3;
the mass ratio of the rhamnolipid, the sophorolipid and the N-acetylgalactosamine is 2: 1: 4;
the mass ratio of the disodium ethylene diamine tetraacetate, the citric acid and the triethanolamine is 5: 10: 1.
10. A method for preparing a biological enzyme complex blocking remover, which is used for preparing the biological enzyme complex blocking remover according to any one of claims 1-9, and comprises the following steps:
obtaining the components in parts by mass, wherein the components comprise 0.1-0.5% of biological enzyme, 3-5% of biological surfactant, 5-10% of biological enzyme stabilizer and the balance of water;
and sequentially adding the biological surfactant, the biological enzyme stabilizer and the biological enzyme into the water, and uniformly mixing to obtain the biological enzyme composite blocking remover.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114507516A (en) * | 2022-04-18 | 2022-05-17 | 胜利油田新海兴达实业集团有限责任公司 | Biological enzyme composite blocking remover and preparation method thereof |
| CN115433557A (en) * | 2022-09-20 | 2022-12-06 | 天津君宇生物科技有限公司 | Preparation method and application of biological enzyme in oilfield production increase |
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| CN116240007A (en) * | 2021-12-08 | 2023-06-09 | 天津大港油田滨港集团博弘石油化工有限公司 | Preparation method of biological enzyme viscosity-reducing oil displacement agent |
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| CN114507516B (en) * | 2022-04-18 | 2022-07-05 | 胜利油田新海兴达实业集团有限责任公司 | Biological enzyme composite blocking remover and preparation method thereof |
| CN115433557A (en) * | 2022-09-20 | 2022-12-06 | 天津君宇生物科技有限公司 | Preparation method and application of biological enzyme in oilfield production increase |
| CN116355603A (en) * | 2023-03-21 | 2023-06-30 | 陕西延长石油（集团）有限责任公司 | Non-ultralow interfacial tension oil displacement agent for improving recovery ratio of low-permeability oil reservoir as well as preparation method and application thereof |
| CN116355603B (en) * | 2023-03-21 | 2024-04-16 | 陕西延长石油（集团）有限责任公司 | Non-ultralow interfacial tension oil displacement agent for improving recovery ratio of low-permeability oil reservoir as well as preparation method and application thereof |
| CN116574495A (en) * | 2023-05-17 | 2023-08-11 | 西安石油大油气科技有限公司 | Biological enzyme-nano-composite sulfonate blocking remover, and preparation method and application thereof |
| CN117447977A (en) * | 2023-12-18 | 2024-01-26 | 东营市百扬石油科技有限责任公司 | Preparation method and application of blocking remover containing biological enzyme |
| CN117447977B (en) * | 2023-12-18 | 2024-02-23 | 东营市百扬石油科技有限责任公司 | Preparation method and application of blocking remover containing biological enzyme |
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