CN112772780A - Hydrolysis method of amino acid fermentation waste and application of amino acid fermentation waste in feed additive - Google Patents
Hydrolysis method of amino acid fermentation waste and application of amino acid fermentation waste in feed additive Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 24
- 150000001413 amino acids Chemical class 0.000 title claims abstract description 23
- 230000007062 hydrolysis Effects 0.000 title claims abstract description 21
- 238000006460 hydrolysis reaction Methods 0.000 title claims abstract description 21
- 239000002921 fermentation waste Substances 0.000 title claims abstract description 14
- 239000003674 animal food additive Substances 0.000 title description 10
- 238000004880 explosion Methods 0.000 claims abstract description 30
- 238000001035 drying Methods 0.000 claims abstract description 28
- 239000000919 ceramic Substances 0.000 claims abstract description 16
- 238000005374 membrane filtration Methods 0.000 claims abstract description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 31
- 108090000623 proteins and genes Proteins 0.000 claims description 31
- 108090000790 Enzymes Proteins 0.000 claims description 18
- 102000004190 Enzymes Human genes 0.000 claims description 18
- 238000002156 mixing Methods 0.000 claims description 16
- 238000000855 fermentation Methods 0.000 claims description 13
- 230000004151 fermentation Effects 0.000 claims description 13
- 239000012528 membrane Substances 0.000 claims description 12
- 239000000126 substance Substances 0.000 claims description 12
- 239000000413 hydrolysate Substances 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 11
- 238000005422 blasting Methods 0.000 claims description 8
- 235000007164 Oryza sativa Nutrition 0.000 claims description 7
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 7
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 7
- 235000009566 rice Nutrition 0.000 claims description 7
- 150000003839 salts Chemical class 0.000 claims description 7
- 108091005508 Acid proteases Proteins 0.000 claims description 5
- 239000002360 explosive Substances 0.000 claims description 5
- 230000003301 hydrolyzing effect Effects 0.000 claims description 5
- 238000004806 packaging method and process Methods 0.000 claims description 5
- 238000007873 sieving Methods 0.000 claims description 5
- 238000005550 wet granulation Methods 0.000 claims description 5
- 108010009736 Protein Hydrolysates Proteins 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 2
- 239000011148 porous material Substances 0.000 claims 1
- 239000002699 waste material Substances 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 3
- 235000018102 proteins Nutrition 0.000 description 27
- 235000001014 amino acid Nutrition 0.000 description 17
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 9
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 241000209094 Oryza Species 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000000751 protein extraction Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000011049 filling Methods 0.000 description 3
- 239000008187 granular material Substances 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000004448 titration Methods 0.000 description 3
- 241000186226 Corynebacterium glutamicum Species 0.000 description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
- 235000019764 Soybean Meal Nutrition 0.000 description 2
- 238000005903 acid hydrolysis reaction Methods 0.000 description 2
- 235000013922 glutamic acid Nutrition 0.000 description 2
- 239000004220 glutamic acid Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000004455 soybean meal Substances 0.000 description 2
- 240000001548 Camellia japonica Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 238000003916 acid precipitation Methods 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 235000018597 common camellia Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000012982 microporous membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002052 molecular layer Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Organic Chemistry (AREA)
- Animal Husbandry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Inorganic Chemistry (AREA)
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Abstract
The invention belongs to the technical field of biology, and discloses a hydrolysis method of amino acid fermentation waste, which is characterized by comprising the following steps: step 1) ceramic membrane filtration, step 2) tube bundle drying, step 3) steam explosion and step 4) enzymolysis. The invention has good hydrolysis effect on the mycoprotein, realizes the reutilization of amino acid waste resources and changes waste into valuables.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a hydrolysis method of amino acid fermentation waste and application of the amino acid fermentation waste in a feed additive.
Background
At present, the economy of China is rapidly developed, the development of some biochemical manufacturing industries of China is effectively promoted under the promotion of the development of the economy and the technology, and in the development of the biochemical industry, the waste generated in the production process of fermentation pharmacy has high concentration and contains difficultly degraded substances and byproducts, so the treatment of the fermentation waste is always a major problem facing biological fermentation enterprises.
At present, most of amino acid waste is treated by methods such as direct combustion, entrusted third party treatment and the like, so that not only is a considerable waste treatment cost imposed on manufacturers every year, but also water-soluble nutrient substances such as amino acids, sugar, mycoprotein and the like in the waste cannot be recycled, and the waste is caused by direct discharge.
The steam explosion principle is that in the high temperature and high pressure process, high saturated steam is instantaneously released into the environment and is rapidly cooled, so that the biological material is damaged to generate a compound with low relative molecular mass, and the steam explosion principle is widely used for material pretreatment. The current steam explosion technology is also applied to the aspect of protein extraction, and the steam explosion can improve the protein extraction rate of the soybean meal, change the physicochemical property and structure of the protein and enhance the hydrophilicity of the protein. In the prior art 1, the technology for extracting the camellia seed protein by combining steam explosion with an alkali-soluble acid precipitation method is researched in 2019 of Chinese oil, and the optimal extraction conditions are determined to be 0.8-2.3 MPa of steam explosion pressure, 30-120 s of steam explosion time, 1: 10 of material-liquid ratio, 40 ℃ of extraction temperature, 10 of pH and 50min of extraction time. In the prior art 2, the influence of steam explosion on the solubility of protein in high-temperature soybean meal and a subsequent protein extraction process are researched in 2013 of the food and fermentation industry by the aid of steam explosion. For the related technology of protein steam explosion, the prior art mostly researches the influence of the steam explosion on the protein extraction rate, and no related literature report on the influence on proteolysis is available.
Disclosure of Invention
In order to solve the problems, the invention provides a method for preparing amino acid fermentation waste, which is simple to operate and high in efficiency and can be used for hydrolysis, a product obtained by the method and application of the product in feed additives.
The invention is realized by the following technical scheme.
A hydrolysis method of amino acid fermentation waste is characterized by comprising the following steps: step 1) ceramic membrane filtration, step 2) tube bundle drying, step 3) steam explosion and step 4) enzymolysis.
Further, the hydrolysis method comprises the following steps:
step 1) ceramic membrane filtration: filtering the amino acid fermentation liquor by a ceramic membrane, and collecting the trapped wet protein;
step 2), tube bundle drying: drying the wet protein collected in the step 1) by adopting a steam tube bundle rotary contact dryer to obtain protein powder;
step 3), steam explosion: placing the protein powder in a steam explosion machine, maintaining the pressure for 30-40s under the condition that the pressure is 1.5-2.0MPa, then suddenly releasing the pressure, and carrying out explosion treatment to obtain an explosive substance;
step 4), enzymolysis: preparing 200U/ml enzyme solution from acid protease, mixing the blasting substance obtained in step 3) and the enzyme solution, adjusting pH to 2.0-4.0, hydrolyzing at 40-45 deg.C for 3-5h, and collecting the hydrolysate.
The hydrolysis process further comprises:
step 5) granulating and drying: uniformly mixing the hydrolysate obtained in the step 4), bran, rice bran, bean cakes and salt, and performing wet granulation; then drying, sieving and packaging to obtain the feed.
Preferably, the first and second electrodes are formed of a metal,
the aperture of the ceramic membrane is 60-70 nm.
Preferably, the first and second electrodes are formed of a metal,
mixing the blasting substance obtained in the step 3) and enzyme solution according to the proportion of 1-2 g: 2-3ml of enzyme solution are mixed evenly.
Preferably, the first and second electrodes are formed of a metal,
uniformly mixing the hydrolysate obtained in the step 4), bran, rice bran, bean cakes and salt according to the mass ratio of 100:50:15:10: 1.
The technical scheme of the invention has the following outstanding advantages and uniqueness:
1. the ceramic membrane filtration has the advantages of high separation efficiency, stable effect, good chemical stability, high mechanical strength, simple separation process, low energy consumption and simple and convenient operation and maintenance.
2. The drying is carried out by adopting a steam tube bundle rotary contact dryer, the processing capacity is large, the fuel consumption is low, the drying cost is low, and the water content of the protein powder is less than or equal to 10 percent.
3. The method provides a feed additive prepared by treating and recycling nutrient substances in the amino acid waste liquid at low cost for amino acid production enterprises, obtains the mycoprotein feed with rich nutrition from the feed additive, effectively recycles resources, realizes resource utilization of wastes, greatly reduces discharge of waste water, avoids pollution of the waste liquid to the environment, is green and environment-friendly, saves considerable sewage treatment cost for the enterprises, comprehensively utilizes the resources, changes waste into valuables, and has good social benefit and economic benefit.
4. The cell wall structure of the thallus is thoroughly broken through steam explosion treatment of the thallus protein, and the shock wave generated by explosion can generate fracture and recombination effects on a molecular layer, so that the three-dimensional structure of the protein is damaged, the loose and disordered state is kept, more enzyme binding sites are exposed, the enzymolysis reaction is facilitated, the use amount of enzyme can be reduced, the enzyme reaction time is shortened, the production efficiency is improved, and the cost is saved. The blasting product is subjected to acid hydrolysis, the hydrolysis rate of the mycoprotein is not greatly influenced, and the blasting treatment of the mycoprotein is not suitable for the subsequent acid hydrolysis. The hydrolysate obtained by enzymolysis of the blasting substances has high ammonia nitrogen content and high nutritive value, and is suitable for feed additives.
Detailed Description
In order to make those skilled in the art better understand the technical solutions in the present application, the present invention will be described more clearly and completely below with reference to specific embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A hydrolysis method of amino acid fermentation waste comprises the following steps:
step 1) ceramic membrane filtration: passing amino acid fermentation broth (fermentation broth for producing glutamic acid by fermentation of Corynebacterium glutamicum) through ceramic membrane, allowing feed liquid to flow in membrane tube, intercepting mycoprotein by micropores with inner aperture of 60nm in membrane tube wall under 0.3Mpa pressure, and collecting intercepted wet protein;
step 2), tube bundle drying: feeding the wet protein collected in the step 1) into a stranding cage, adopting a steam tube bundle rotary contact dryer to enable the tube bundle rotating slowly to axially convey and feed in the whole dryer, and drying the wet protein in contact with a heating pipe, wherein the filling rate of the material is 40%, the drying temperature is 70 ℃, and the dried protein powder is obtained, and the protein content is 78%;
step 3), steam explosion: placing the protein powder in a steam explosion machine, maintaining the pressure for 30s under the condition that the pressure is 2.0MPa, then suddenly releasing the pressure, and carrying out explosion treatment to obtain an explosive;
step 4), enzymolysis: preparing 200u/ml enzyme solution by using acid protease, and mixing the blasting substance obtained in the step 3) and the enzyme solution according to the proportion of 1 g: mixing 2ml of enzyme solution, adjusting pH to 3.0, hydrolyzing at 45 deg.C for 4 hr, and collecting hydrolysate which can be used as feed additive; through detection, the ammonia nitrogen content (formaldehyde titration method) is 13.7 g/L;
step 5) granulating and drying: uniformly mixing the hydrolysate, the bran, the rice bran, the bean cake and the salt in the step 4) according to a ratio of 100:50:15:10:1, and performing wet granulation; then drying to keep the water content of the granules below 5%, sieving, and packaging to obtain the feed.
Example 2
A hydrolysis method of amino acid fermentation waste comprises the following steps:
step 1) ceramic membrane filtration: passing amino acid fermentation broth (fermentation broth for producing threonine by fermenting Escherichia coli) through ceramic membrane, allowing feed liquid to flow in membrane tube, intercepting mycoprotein by microporous membrane tube wall with inner aperture of 70nm under 0.4Mpa, and collecting intercepted wet protein;
step 2), tube bundle drying: feeding the wet protein collected in the step 1) into a stranding cage, adopting a steam tube bundle to rotate and contact a dryer, enabling the tube bundle rotating slowly to axially convey and feed in the whole dryer, and drying the wet protein in contact with a heating pipe, wherein the filling rate of the material is 50%, the drying temperature is 65 ℃, and the dried protein powder is obtained, and the protein content is 76%;
step 3), steam explosion: placing the protein powder in a steam explosion machine, maintaining the pressure for 40s under the condition that the pressure is 1.5MPa, then suddenly releasing the pressure, and carrying out explosion treatment to obtain an explosive;
step 4), enzymolysis: preparing 200u/ml enzyme solution by using acid protease, and mixing the blasting substance obtained in the step 3) and the enzyme solution according to the proportion of 1 g: mixing 2ml of enzyme solution, adjusting pH to 3.0, hydrolyzing at 45 deg.C for 5 hr, and collecting hydrolysate which can be used as feed additive; through detection, the ammonia nitrogen content (formaldehyde titration method) is 12.6 g/L;
step 5) granulating and drying: uniformly mixing the hydrolysate, the bran, the rice bran, the bean cake and the salt in the step 4) according to a ratio of 100:50:15:10:1, and performing wet granulation; then drying to keep the water content of the granules below 5%, sieving, and packaging to obtain the feed.
Example 3
A hydrolysis method of amino acid fermentation waste comprises the following steps:
step 1) ceramic membrane filtration: passing amino acid fermentation broth (fermentation broth for producing glutamic acid by fermentation of Corynebacterium glutamicum) through ceramic membrane, allowing feed liquid to flow in membrane tube, intercepting mycoprotein by micropores with inner aperture of 60nm in membrane tube wall under 0.3Mpa pressure, and collecting intercepted wet protein;
step 2), tube bundle drying: feeding the wet protein collected in the step 1) into a stranding cage, adopting a steam tube bundle rotary contact dryer to enable the tube bundle rotating slowly to axially convey and feed in the whole dryer, and drying the wet protein in contact with a heating pipe, wherein the filling rate of the material is 40%, the drying temperature is 70 ℃, and the dried protein powder is obtained, and the protein content is 78%;
step 3) enzymolysis: preparing 200u/ml enzyme solution by using acid protease, and mixing the protein powder obtained in the step 2) and the enzyme solution according to the proportion of 1 g: mixing 2ml of enzyme solution, adjusting pH to 3.0, hydrolyzing at 45 deg.C for 4 hr, and collecting hydrolysate which can be used as feed additive; through detection, the ammonia nitrogen content (formaldehyde titration method) is 4.1 g/L;
step 4), granulating and drying: uniformly mixing the hydrolysate, the bran, the rice bran, the bean cake and the salt in the step 3) according to a ratio of 100:50:15:10:1, and performing wet granulation; then drying to keep the water content of the granules below 5%, sieving, and packaging to obtain the feed.
Example 4
Influence of steam explosion pressure maintaining time on the content of ammonia nitrogen in the hydrolysate.
Taking the hydrolysis method of example 1 as an implementation mode, setting the pressure maintaining time of steam explosion as 10, 20, 30, 40, 50 and 60 respectively, and setting the unit as s, wherein the ammonia nitrogen content in each hydrolysis liquid is shown in table 1:
TABLE 1
| Time s | 10 | 20 | 30 | 40 | 50 | 60 |
| The ammonia nitrogen content in the hydrolysate is g/L | 8.6 | 10.4 | 13.7 | 13.8 | 13.9 | 13.9 |
While the invention has been described with reference to specific embodiments, it will be apparent to those skilled in the art that the invention is not limited thereto, and that various changes and modifications can be made without departing from the spirit and scope of the invention.
Claims (6)
1. A hydrolysis method of amino acid fermentation waste is characterized by comprising the following steps: step 1) ceramic membrane filtration, step 2) tube bundle drying, step 3) steam explosion and step 4) enzymolysis.
2. The hydrolysis process according to claim 1, characterized in that it comprises the following steps:
step 1) ceramic membrane filtration: filtering the amino acid fermentation liquor by a ceramic membrane, and collecting the trapped wet protein;
step 2), tube bundle drying: drying the wet protein collected in the step 1) by adopting a steam tube bundle rotary contact dryer to obtain protein powder;
step 3), steam explosion: placing the protein powder obtained in the step 2) into a steam explosion machine, maintaining the pressure for 30-40s under the condition that the pressure is 1.5-2.0MPa, then releasing the pressure suddenly, and carrying out explosion treatment to obtain an explosive substance;
step 4), enzymolysis: preparing 200U/ml enzyme solution from acid protease, mixing the blasting substance obtained in step 3) and the enzyme solution, adjusting pH to 2.0-4.0, hydrolyzing at 40-45 deg.C for 3-5h, and collecting the hydrolysate.
3. The hydrolysis method according to claim 2, further comprising:
step 5) granulating and drying: uniformly mixing the hydrolysate obtained in the step 4), bran, rice bran, bean cakes and salt, and performing wet granulation; then drying, sieving and packaging to obtain the feed.
4. The hydrolysis method according to claim 2, wherein the ceramic membrane has a pore size of 60 to 70 nm.
5. The hydrolysis method according to claim 2, wherein the ratio of the explosive and the enzyme solution obtained in step 3) is in the range of 1 to 2 g: mixing at a ratio of 2-3 ml.
6. The hydrolysis method according to claim 3, wherein the hydrolysate, the bran, the rice bran, the bean cake and the salt in the step 4) are uniformly mixed according to a mass ratio of 100:50:15:10: 1.
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2020
- 2020-12-27 CN CN202011571129.XA patent/CN112772780A/en active Pending
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| CN104996726A (en) * | 2015-07-10 | 2015-10-28 | 河南恩赛姆生物科技有限公司 | A functional feed additive production method using Hermetia illucens larvae |
| CN107467398A (en) * | 2017-08-31 | 2017-12-15 | 安徽金丰源畜牧科技有限公司 | A kind of biological straw particle chicken feed of explosion enzymolysis processing |
| CN107495394A (en) * | 2017-09-11 | 2017-12-22 | 唐山润泽粮油食品有限公司 | A kind of steam blasting is modified the method with enzymolysis coupling extraction dietary fibers from peanuts |
| CN108707631A (en) * | 2018-05-02 | 2018-10-26 | 莲花健康产业集团股份有限公司 | A kind of glutamic acid fermentation discards the reuse method of thalline |
| CN110423691A (en) * | 2019-08-09 | 2019-11-08 | 宜昌三峡普诺丁生物制药有限公司 | A kind of method that amino acid zymotic fluid bacteria residue prepares mycoprotein medicinal extract |
| CN110937725A (en) * | 2019-12-22 | 2020-03-31 | 赵兰坤 | Method for restoring fermentation wastewater and preparing feed by utilizing mycoprotein |
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