CN112763562A - Preparation method of branch-shaped walking machine aptamer electrochemical sensor for adenosine triphosphate detection - Google Patents
Preparation method of branch-shaped walking machine aptamer electrochemical sensor for adenosine triphosphate detection Download PDFInfo
- Publication number
- CN112763562A CN112763562A CN202110120544.1A CN202110120544A CN112763562A CN 112763562 A CN112763562 A CN 112763562A CN 202110120544 A CN202110120544 A CN 202110120544A CN 112763562 A CN112763562 A CN 112763562A
- Authority
- CN
- China
- Prior art keywords
- branch
- carbon nano
- walking machine
- shaped
- adenosine triphosphate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nanotechnology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Engineering & Computer Science (AREA)
- Electrochemistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to a method for preparing a branch-shaped walking machine aptamer electrochemical sensor for detecting adenosine triphosphate, which comprises the following steps: a spiked spherical bimetallic oxide/functionalized carbon nano composite material is prepared by a hydrothermal synthesis method, a base complementary pairing is designed to construct a branch-shaped walking machine, the branch-shaped walking machine/spiked spherical bimetallic oxide/functionalized carbon nano composite material/thin-film gold electrode is constructed by covalent bonding, the content of target adenosine triphosphate is reflected by using signal change generated by signal molecule-labeled T-DNA, and a signal-reduced electrochemical aptamer sensor for detecting adenosine triphosphate is obtained. Compared with other electrochemical sensors for detecting the content of adenosine triphosphate, the prepared branch-shaped walking machine aptamer electrochemical sensor has the advantages of high sensitivity, good repeatability and high accuracy.
Description
Technical Field
The invention relates to a preparation method of a branch-shaped walking machine aptamer electrochemical sensor for adenosine triphosphate detection, in particular to a preparation method of a branch-shaped walking machine/spiky bimetallic oxide/functionalized carbon nano composite material/gold electrode.
Background
Adenosine Triphosphate (ATP), the main energy source of cells, is involved in various biological processes including cell function, muscle contraction, neurotransmission, platelet function, membrane transport and vasodilation, and is called the "molecular energy currency organism" of all organisms. Plays a key role in regulating cellular metabolism and biochemical pathways in cellular physiology. It has also been used as an indicator of cell viability and cell damage, playing a vital role in biochemical reactions in regulating cell metabolism. Therefore, intracellular ATP concentration is closely related to the number of living cells. ATP is a good biomarker, which is directly detected sensitively and reliably to monitor the presence of pathological events, which is of great significance in the food industry and in the medical community. Traditional ATP detection methods are mainly based on high performance liquid chromatography, mass spectrometry and bioluminescence, however, these methods are complex and technically demanding. Nowadays, various novel analytical methods including chemiluminescence, surface-enhanced raman scattering, fluorescence, etc. have been widely explored to determine the amount of ATP, and although these methods can detect ATP with high sensitivity, they suffer from the disadvantages of time consuming, expensive equipment and inconvenient field operations. Therefore, the development of a faster, more convenient and more sensitive ATP detection method has important practical significance.
Disclosure of Invention
The invention relates to a method for preparing a branch-shaped walking machine aptamer electrochemical sensor for detecting adenosine triphosphate.
A method for preparing a branch-shaped walking machine aptamer electrochemical sensor for detecting adenosine triphosphate comprises the following steps:
the preparation method of the thorn-ball-shaped bimetal oxide/functionalized carbon nano composite material comprises the following steps: preparing a functionalized carbon nano material by using a hydrothermal synthesis method, taking 25-35 mg of the carbon nano material, dissolving the carbon nano material in 15-25 ml of water, and carrying out ultrasonic treatment for 50-70 min. And then slowly adding 1.5-5.5 ml of 2-5% of a functional reagent by using a magnetic stirrer at 400-500 rpm, carrying out oil bath heating on the stirred mixed solution at 125-145 ℃ for 2-4 h, centrifuging the obtained product at 8000-10000 rpm (5-10 min), washing with water for 2-5 times, and drying at 50-100 ℃ to obtain the functional carbon nano material. Then dissolving 100-120 mg of metal chloride solution, 170-190 mg of rare earth metal solution and 170-190 mg of organic solvent A in 15-25 mL of mixed liquid (H)2The volume ratio of O to the organic solvent B is 1: 1-1: 3), then adding 15-25 mL of 1-2 mg/mL of the functionalized carbon nano-material suspension into the prepared mixed solution, and carrying out ultrasonic treatment for 35-45 minutes at room temperature. Transferring the obtained solution into a high-pressure reaction kettle, and heating the solution in an electrothermal static drying oven at 85-95 ℃ for 10-24 hours. And after the reaction is finished, repeatedly washing the obtained solid precipitate for 3-5 times by using deionized water and an organic solvent C. And drying the final solid product in an oven at 55-75 ℃ for 25-40 minutes. And finally, annealing for 0.5-1.5 hours in a muffle furnace at 220-280 ℃ to obtain the spiky bimetallic oxide/functionalized carbon nano composite material.
Preparing the branch-shaped walking machine: adding 90-110 mu L of 90-110 mu mol/L adenosine triphosphate aptamer (Apt), DNA-1 (60-75 mu L60-75 mu M), swing arm chain DNA-2 (5-10 mu M) and 10-15 mu L of substrate chain DNA-3 (8-13 mu M) into 20-30 mM buffer solution D, and incubating at 30-45 ℃ for 1-3 h to obtain the branch-shaped walking machine.
The preparation of the aptamer/spiky bimetallic oxide/functionalized carbon nano composite material/gold electrode comprises the following steps: preparing a thin-film gold electrode by utilizing a magnetron sputtering coating technology, coating a reference electrode with silver/silver chloride slurry by adopting a screen printing technology, dropwise coating 3-8 mu L of a thorn-ball-shaped bimetal oxide/functionalized carbon nano composite material solution on the surface of a working electrode by using a trace liquid taking device, drying for 3-6 min under an infrared lamp, transferring 10-15 mu L of a hairpin-shaped binding chain H-DNA for incubation for 1-3H at 30-45 ℃, fixing the hairpin-shaped binding chain H-DNA on the surface of the electrode through a covalent bond, transferring 7-12 mu L of the prepared branch-shaped walking machine to the surface of the electrode, incubating for 1-3H at 30-45 ℃, then transferring 0.5-2 mM of a sealing agent to the surface of the electrode, incubating for 20-40 min at 30-40 ℃, and sealing empty electric active sites. And then transferring 5-10 mu L of a mixture containing ATP with different concentrations and 1-5U of shear enzyme to the surface of a gold electrode, dropwise adding the mixture to the surface of the gold electrode, incubating for 60-120 min at 30-60 ℃, dropwise adding 5-10 mu L of signal molecule labeled T-DNA (5-10 mu M) to the surface of the gold electrode, and incubating for 60-120 min at 30-60 ℃, thus obtaining the branch-shaped walking machine/thorn-ball-shaped bimetal oxide/functionalized carbon nanocomposite/gold electrode.
The electrochemical sensor uses a branch-shaped walking machine/thorn ball-shaped bimetal oxide/functionalized carbon nano composite material/gold electrode as a working electrode, and reflects the content of a target ATP (adenosine triphosphate) by using signal change generated by signal molecule labeled T-DNA (deoxyribonucleic acid), so that the electrochemical aptamer sensor for detecting the adenosine triphosphate with a reduced signal is obtained.
The thorn-ball-shaped bimetal is one or more of gold platinum particles, gold palladium particles, platinum palladium particles and cobalt lanthanum particles;
the carbon nano material and the functionalized carbon nano material are one or more of multi-walled carbon nano tubes, graphene oxide, reduced graphene oxide, biomass porous carbon, polydimethy diallyl ammonium chloride graphene, poly-dopamine graphene and graphene;
the functional reagent is one or more of polyetherimide, polyethyleneimine, poly diallyl dimethyl ammonium chloride and polydopamine;
the metal chloride solution is one or more of potassium chloroplatinate, chloroauric acid, sodium chloropalladate, cobalt dichloride tetrahydrate and phosgene;
the rare earth metal solution is one or more of lanthanum chloride heptahydrate, scandium chloride, cerium chloride and neodymium chloride;
the organic solvent A, the organic solvent B and the organic solvent C are one or more of styrene, perchloroethylene, trichloroethylene, ethanol, ethylene glycol ether and triethanolamine;
the buffer solution A, the buffer solution B, the buffer solution C and the buffer solution D are one or more of HCl-Tris buffer solution, PBS buffer solution, triethanolamine, HEPES solution and 2- (N-morpholine) ethanesulfonic acid;
the blocking agent is one of 6-mercaptohexanol, bovine serum albumin and hexanethiol.
The said shear enzyme is one or more of peroxidase, Nb.BbvcI, DNase I;
the signal molecule is one or more of ferrocene, methylene blue and neutral red.
In the sensor, the branch-shaped walking machine/spiky bimetallic oxide/functionalized carbon nano composite material/thin film gold electrode is used as a working electrode, and compared with other electrochemical sensors for detecting the ATP content, the prepared branch-shaped walking machine aptamer electrochemical sensor has the advantages of high sensitivity, good repeatability and high accuracy.
Detailed Description
The invention is described below with reference to specific examples:
example 1
The method comprises the following specific steps:
(1) preparing a Co-La oxide/PEI-rGO composite material: preparing the functionalized carbon nano material by using a hydrothermal synthesis method, taking 30mg of the carbon nano material, dissolving the carbon nano material in 20 ml of water, and carrying out ultrasonic treatment for 60 min. Then 2 ml of 3% amino functional solution was slowly added with a magnetic stirrer at 450 rpm, the stirred mixed solution was oil-bath heated at 135 ℃ for 3 h, the obtained product was centrifuged at 8500 rpm (6 min) and washed 3 times with water, and dried at 60 ℃ to obtain a functionalized carbon nanomaterial. 110 mg of CoCl2·4H2O, 180 mg of LaCl3·7H2O and 180 mg of urea were dissolved in 20 mL of a mixed solution (H)2O and ethanol in a volume ratio of 1: 1), then preparing a mixed solution, adding 20 mL of 1.5 mg/mL PEI-rGO suspension into the mixed solution, and performing ultrasonic treatment at room temperatureAnd processing for 40 minutes. The resulting solution was transferred to a 100 mL autoclave and heated at 90 ℃ for 16h in an electrically heated static oven. After the reaction was completed, the obtained solid precipitate was repeatedly washed 3 times with deionized water and ethanol. The final solid product was dried in an oven at 60 ℃ for 30 minutes. And finally, annealing for 1 h in a muffle furnace at 250 ℃ to obtain the Co-La oxide/PEI-rGO composite material.
(2) Preparing a branch-shaped walking machine: 90. mu.L of 90. mu. mol/L adenosine triphosphate aptamer (Apt), DNA-1 (60. mu.L 60. mu.M), swing arm strand DNA-2 (5. mu.M) and 10. mu.L of substrate strand DNA-3 (8. mu.M) were added to 20 mM buffer solution D, and incubated at 30 ℃ for 1 hour to obtain a brush-type walking machine.
(3) Preparing a branch type walking machine/Co-La oxide/PEI-rGO composite material/thin film gold electrode: preparing a thin-film gold electrode by utilizing a magnetron sputtering coating technology, coating a reference electrode with silver/silver chloride slurry by adopting a screen printing technology, dropwise coating 3 mu L of Co-La oxide/PEI-rGO composite material solution on the surface of a working electrode by using a trace liquid taking device, drying for 3 min under an infrared lamp, transferring 10 mu L of hairpin-shaped binding chain H-DNA, incubating for 1H at 30 ℃, fixing on the surface of the electrode through a covalent bond, transferring 7 mu L of the prepared branch-shaped walking machine, dropwise adding the prepared branch-shaped walking machine to the surface of the electrode, incubating for 1H at 30 ℃, then transferring 0.5 mM MCH, dropwise adding the MCH to the surface of the electrode, incubating for 20 min at 30 ℃, and sealing empty residual electroactive sites. And then 5 mu L of a mixture containing ATP with different concentrations and 1U Nb, BbvcI is dripped onto the surface of the gold electrode to incubate for 60 min at 30 ℃, 5 mu L of signal molecule labeled T-DNA (5 mu M) is dripped onto the surface of the gold electrode to incubate for 60 min at 30 ℃, and the branch-shaped walking machine/Co-La oxide/PEI-rGO composite material/thin film gold electrode is obtained.
(4) The electrochemical sensor uses an aptamer/Co-La oxide/PEI-rGO composite material/thin film gold electrode as a working electrode, and reflects the content of a target ATP (adenosine triphosphate) by using signal change generated by ferrocene-labeled T-DNA (deoxyribonucleic acid), so that the electrochemical aptamer sensor for detecting the ATP with a reduced signal is obtained.
The prepared electrochemical sensor has high accuracy in ATP detection and wide linear range (1×10-10~1×10-2μ mol/L), low detection lower limit (5X 10)-11μ mol/L). Meanwhile, the detection result of the actual sample shows that the prepared sensor has very good practical application value.
The above examples are intended to illustrate the invention, but not to limit it. Many modifications and variations of the present invention are possible in light of the above teachings. Within the scope of the appended claims, the present invention may be realized in other ways than those described above, and it is within the scope of the claims to select other reagent materials, adjust incubation time, etc.
Claims (4)
1. A method for preparing a branch-type walking machine aptamer electrochemical sensor for adenosine triphosphate detection is characterized by comprising the following steps:
(1) preparing a thorn-ball-shaped bimetal oxide/functionalized carbon nano composite material: preparing a functionalized carbon nano material by using a hydrothermal synthesis method, taking 25-35 mg of the carbon nano material, dissolving the carbon nano material in 15-25 ml of water, and carrying out ultrasonic treatment for 50-70 min; slowly adding 1.5-5.5 ml of 2-5% of a functional reagent under magnetic stirring at 400-500 rpm, performing oil bath heating on the stirred mixed solution at 125-145 ℃ for 2-4 h, centrifuging the obtained product at 8000-10000 rpm (5-10 min), washing with water for 2-5 times, and drying at 50-100 ℃ to obtain a functional carbon nano material; the preparation method of the thorn-ball-shaped bimetal oxide/functionalized carbon nano composite material comprises the steps of dissolving 100-120 mg of metal chloride solution, 170-190 mg of rare earth metal solution and 170-190 mg of organic solvent A in 15-25 mL of mixed liquid (H)2The volume ratio of O to the organic solvent B is 1: 1-1: 3), then adding 15-25 mL of 1-2 mg/mL of functionalized carbon nano-material suspension into the prepared mixed solution, and carrying out ultrasonic treatment for 35-45 min at room temperature; transferring the obtained solution into a high-pressure reaction kettle, and heating the solution in an electrothermal static drying oven at 85-95 ℃ for 10-24 hours; after the reaction is finished, repeatedly washing the obtained solid precipitate for 3-5 times by using deionized water and an organic solvent C; drying the final solid product in an oven at the temperature of 55-75 ℃ for 25-40 min; finally, byAnnealing for 0.5-1.5 hours in a muffle furnace at 220-280 ℃ to obtain a thorn-ball-shaped bimetal oxide/functionalized carbon nano composite material;
(2) preparing a branch-shaped walking machine: adding 90-110 mu L of 90-110 mu mol/L adenosine triphosphate aptamer (Apt), DNA-1 (60-75 mu L60-75 mu M), swing arm chain DNA-2 (5-10 mu M) and 10-15 mu L of substrate chain DNA-3 (8-13 mu M) into 20-30 mM buffer solution D, and incubating for 1-3 h at 30-45 ℃ to obtain a branch-shaped walking machine;
(3) preparing a branch-shaped walking machine/thorn-ball-shaped bimetal oxide/functionalized carbon nano composite material/thin-film gold electrode: preparing a thin-film gold electrode by utilizing a magnetron sputtering coating technology, coating a reference electrode with silver/silver chloride slurry by adopting a screen printing technology, dropwise coating 3-8 mu L of a thorn-ball-shaped bimetal oxide/functionalized carbon nano composite material solution on the surface of a working electrode by using a trace liquid taking device, drying for 3-6 min under an infrared lamp, transferring 10-15 mu L of a hairpin-shaped binding chain H-DNA for incubation for 1-3H at 30-45 ℃, fixing the hairpin-shaped binding chain H-DNA on the surface of the electrode through a covalent bond, transferring 7-12 mu L of the prepared branch-shaped walking machine to the surface of the electrode, incubating for 1-3H at 30-45 ℃, then transferring 0.5-2 mM of a sealing agent to the surface of the electrode, incubating for 20-40 min at 30-40 ℃, and sealing empty electric active sites; then transferring 5-10 mu L of a mixture containing ATP with different concentrations and 1-5U of shear enzyme to the surface of a gold electrode, dropwise adding the mixture to the surface of the gold electrode, incubating for 60-120 min at 30-60 ℃, dropwise adding 5-10 mu L of signal molecule labeled T-DNA (5-10 mu M) to the surface of the gold electrode, and incubating for 60-120 min at 30-60 ℃, thus obtaining a branch-shaped walking machine/thorn-ball-shaped bimetal oxide/functionalized carbon nano composite material/gold electrode;
(4) the electrochemical sensor uses a branch-shaped walking machine/thorn-ball-shaped bimetal oxide/functionalized carbon nano composite material/thin-film gold electrode as a working electrode, and reflects the content of a target ATP (adenosine triphosphate) by using signal change generated by signal molecule labeled T-DNA (deoxyribonucleic acid), so that the electrochemical aptamer sensor for detecting the ATP with a reduced signal is obtained.
2. The method for preparing a branch-type walking machine aptamer electrochemical sensor for adenosine triphosphate detection according to claim 1, wherein in the step (1), the spiky bimetallic particles are one or more of gold platinum particles, gold palladium particles, platinum palladium particles and cobalt lanthanum particles; the carbon nano material and the functionalized carbon nano material are one or more of multi-walled carbon nano tubes, graphene oxide, reduced graphene oxide, biomass porous carbon, polydimethy diallyl ammonium chloride graphene, poly-dopamine graphene and graphene; the functional reagent is one or more of polyetherimide, polyethyleneimine, poly diallyl dimethyl ammonium chloride and polydopamine; the metal chloride solution is one or more of potassium chloroplatinate, chloroauric acid, sodium chloropalladate, cobalt dichloride tetrahydrate and phosgene; the rare earth metal solution is one or more of lanthanum chloride heptahydrate, scandium chloride, cerium chloride and neodymium chloride; the organic solvent A, the organic solvent B and the organic solvent C are one or more of styrene, perchloroethylene, trichloroethylene, ethanol, urea, ethylene glycol ether and triethanolamine.
3. The method for preparing a branch-type walking machine aptamer electrochemical sensor for adenosine triphosphate detection according to claim 1, wherein in the step (2), the buffer solution A, the buffer solution B, the buffer solution C and the buffer solution D are one or more of Tris-HCl buffer solution, PBS buffer solution, triethanolamine, HEPES solution and 2- (N-morpholine) ethanesulfonic acid; .
4. The method for preparing a branch-type walking machine aptamer electrochemical sensor for adenosine triphosphate detection according to claim 1, wherein in the step (3), the blocking agent is one of 6-mercaptohexanol, bovine serum albumin and hexanethiol; the said shear enzyme is one or more of peroxidase, Nb.BbvcI, DNase I; the signal molecule is one or more of ferrocene, methylene blue and neutral red.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110120544.1A CN112763562B (en) | 2021-01-28 | 2021-01-28 | Preparation method of branch-shaped walking machine aptamer electrochemical sensor for adenosine triphosphate detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110120544.1A CN112763562B (en) | 2021-01-28 | 2021-01-28 | Preparation method of branch-shaped walking machine aptamer electrochemical sensor for adenosine triphosphate detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112763562A true CN112763562A (en) | 2021-05-07 |
| CN112763562B CN112763562B (en) | 2022-06-14 |
Family
ID=75706477
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110120544.1A Active CN112763562B (en) | 2021-01-28 | 2021-01-28 | Preparation method of branch-shaped walking machine aptamer electrochemical sensor for adenosine triphosphate detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112763562B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113884556A (en) * | 2021-10-15 | 2022-01-04 | 河南工业大学 | Preparation method of ratio type electrochemical sensor for ochratoxin A detection |
| CN115479982A (en) * | 2022-11-04 | 2022-12-16 | 唐山学院 | Multi-channel chemical impedance sensor and manufacturing and using methods thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103792328A (en) * | 2014-01-13 | 2014-05-14 | 宁波大学 | Method for detecting ractopamine content in food |
| CN110849867A (en) * | 2019-12-06 | 2020-02-28 | 东北师范大学 | Electroluminescent sensor based on double-hairpin structure and construction method and application thereof |
| CN111398391A (en) * | 2020-05-18 | 2020-07-10 | 河南工业大学 | Preparation method of electrochemical sensor for detecting T-2 toxin residue |
| CN111398396A (en) * | 2020-05-22 | 2020-07-10 | 河南工业大学 | Heavy metal Hg2+And Pb2+Preparation method of electrochemical sensor capable of detecting simultaneously |
-
2021
- 2021-01-28 CN CN202110120544.1A patent/CN112763562B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103792328A (en) * | 2014-01-13 | 2014-05-14 | 宁波大学 | Method for detecting ractopamine content in food |
| CN110849867A (en) * | 2019-12-06 | 2020-02-28 | 东北师范大学 | Electroluminescent sensor based on double-hairpin structure and construction method and application thereof |
| CN111398391A (en) * | 2020-05-18 | 2020-07-10 | 河南工业大学 | Preparation method of electrochemical sensor for detecting T-2 toxin residue |
| CN111398396A (en) * | 2020-05-22 | 2020-07-10 | 河南工业大学 | Heavy metal Hg2+And Pb2+Preparation method of electrochemical sensor capable of detecting simultaneously |
Non-Patent Citations (3)
| Title |
|---|
| BEHZAD REZAEI 等: "An ultrasensitive and selective electrochemical aptasensor based on rGOMWCNTs/Chitosan/carbon quantum dot for the detection of lysozyme", 《BIOSENSORS AND BIOELECTRONICS》 * |
| SEAN D. MASON 等: "Emerging bioanalytical applications of DNA walkers", 《TRENDS IN ANALYTICAL CHEMISTRY》 * |
| 闫艳霞 等: "用于三磷酸腺苷检测的电流型适体传感器", 《微纳电子技术》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113884556A (en) * | 2021-10-15 | 2022-01-04 | 河南工业大学 | Preparation method of ratio type electrochemical sensor for ochratoxin A detection |
| CN113884556B (en) * | 2021-10-15 | 2023-12-15 | 河南工业大学 | Preparation method of ratio-type electrochemical sensor for detecting ochratoxin A |
| CN115479982A (en) * | 2022-11-04 | 2022-12-16 | 唐山学院 | Multi-channel chemical impedance sensor and manufacturing and using methods thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112763562B (en) | 2022-06-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ma et al. | Copper-mediated DNA-scaffolded silver nanocluster on–off switch for detection of pyrophosphate and alkaline phosphatase | |
| Su et al. | Electrochemical Sensors Using Two‐Dimensional Layered Nanomaterials | |
| Qiao et al. | Enhancing the electrochemiluminescence of luminol by chemically modifying the reaction microenvironment | |
| Dutta et al. | An ultrasensitive enzyme-free electrochemical immunosensor based on redox cycling amplification using methylene blue | |
| CN112763562B (en) | Preparation method of branch-shaped walking machine aptamer electrochemical sensor for adenosine triphosphate detection | |
| Rabai et al. | Development of a label-free electrochemical aptasensor based on diazonium electrodeposition: Application to cadmium detection in water | |
| CN111208178B (en) | Method for constructing electrochemical luminescence sensor based on double amplification of perylene tetracarboxylic acid signal by cobalt-based metal organic framework | |
| Wang et al. | Hydroxylamine amplified gold nanoparticle-based aptameric system for the highly selective and sensitive detection of platelet-derived growth factor | |
| Qiu et al. | A label-free electrochemical immunoassay for IgG detection based on the electron transfer | |
| Shi et al. | Self-powered dual-mode sensing strategy based on graphdiyne and DNA nanoring for sensitive detection of tumor biomarker | |
| Liu et al. | A novel aptamer-mediated CuInS 2 quantum dots@ graphene oxide nanocomposites-based fluorescence “turn off–on” nanosensor for highly sensitive and selective detection of kanamycin | |
| Zhang et al. | A novel electrochemical aptamer biosensor based on tetrahedral DNA nanostructures and catalytic hairpin assembly for CEA detection | |
| Wang et al. | A versatile pdru bimetallic nanoenzyme-integrated enzyme-linked immunosorbent assay for highly sensitive Escherichia coli O157: H7 detection | |
| Liu et al. | Label‐Free Electrochemiluminescence Aptasensor for Highly Sensitive Detection of Acetylcholinesterase Based on Au‐Nanoparticle‐Functionalized g‐C3N4 Nanohybrid | |
| Zhang et al. | Prussian blue nanoparticle-labeled aptasensing platform on graphene oxide for voltammetric detection of α-fetoprotein in hepatocellular carcinoma with target recycling | |
| Liu et al. | An antifouling interface integrated with HRP-based amplification to achieve a highly sensitive electrochemical aptasensor for lysozyme detection | |
| Liao et al. | Label-free electrochemical homogeneous detection of the depression marker human apolipoprotein A4 based on proximity hybridization triggered rolling circle amplification | |
| CN106093390B (en) | A kind of PtCu@g C3N4The preparation method and application of the electrochemical immunosensor of/rGO marks | |
| Li et al. | An “off-on” electrochemiluminescence aptasensor for microcystin-LR assay based on the resonance energy transfer from PTCA/NH 2-MIL-125 (Ti) to gold nanoparticles | |
| Fan et al. | An “off–on” electrochemiluminescence aptasensor for determination of lincomycin based on CdS QDs/carboxylated g-C3N4 | |
| Zhang et al. | FeS2− AuNPs nanocomposite as mimicking enzyme for constructing signal‐off sandwich‐type electrochemical immunosensor based on electroactive nickel hexacyanoferrate as matrix | |
| Duan et al. | Ultrasensitive electrochemiluminescent aptasensor for trace detection of kanamycin based-on novel semi-sandwich gadolinium phthalocyanine complex and dysprosium metal-organic framework | |
| Chien et al. | A Nonenzymatic Amperometric Method for Fructosyl‐Valine Sensing Using Ferroceneboronic Acid | |
| Eshlaghi et al. | A label-free electrochemical aptasensor based on screen printed carbon electrodes with gold nanoparticles-polypyrrole composite for detection of cardiac troponin I | |
| Xiong et al. | A DNA dendrimer amplified electrochemical immunosensing method for highly sensitive detection of prostate specific antigen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |