CN111398391A - Preparation method of electrochemical sensor for detecting T-2 toxin residue - Google Patents
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Abstract
The invention relates to a preparation method of an electrochemical aptamer sensor for detecting T-2 toxin residue, which comprises the following steps: preparing a functionalized carbon nano material by a microwave synthesis method, preparing a sulfur-doped metal nano sheet/carbon nano material composite material by a hydrothermal synthesis method, taking a proper amount of sulfur-doped metal nano sheet/functionalized carbon nano composite material/aptamer, and combining the proper amount of sulfur-doped metal nano sheet/functionalized carbon nano composite material/aptamer on a gold electrode in a covalent bonding mode to prepare a sulfur-doped metal nano sheet/functionalized carbon nano composite material/aptamer/gold electrode, taking a platinum wire electrode as a counter electrode and saturated silver chloride as a reference electrode, and obtaining the electrochemical aptamer sensor for detecting the residual T-2 toxin through the change of a signal molecule response signal. Compared with the traditional T-2 toxin detection method, the method has the advantages of high response speed, low detection limit, high sensitivity, good repeatability and high accuracy.
Description
Technical Field
The invention relates to a preparation method of an electrochemical aptamer sensor for detecting T-2 toxin residue, in particular to a preparation method of an electrochemical aptamer sensor for signal amplification based on a sulfur-doped metal nanosheet/functionalized carbon nanocomposite.
Background
The T-2 toxin is a trichothecene with thermal stability generated by fusarium, is widely found in the nature and is one of main toxins polluting field crops and stored grains, a report of China shows that the occurrence rate of the T-2 toxin in 420 feed samples is as high as 79.5%, the T-2 toxin can pollute animal-derived food through food chain and biological enrichment and propagation, and further forms a potential threat to human health, the T-2 toxin can cause hematopoiesis of human and animals, gastrointestinal tract and reproductive dysfunction, the T-2 toxin can interact with aflatoxin to generate synergistic toxicity, meanwhile, the T-2 toxin can be used as the most dangerous food pollution source naturally existing like food in the FAQ and World Health Organization (WHO) in combination with the FAQ, the T-2 toxin is especially important for establishing a T-2 toxin detection method, such as a fluorescence determination method, a gas phase-transfer detection method (a single-labeled protein-labeled by a high-specificity chromatography), and a high-specificity detection method of a nano aptamer-2 aptamer, and a high-2 aptamer-2 protein-based on-specific aptamer-based on-substrate-electrochemical synthesis and a high-sensitivity, and a high-sensitivity test method of a high-sensitivity, and a high-sensitivity of a nano-electrochemical-molecular-substrate-electrochemical-substrate-metal-based nano-element, and a high-sensitivity of a high-electrochemical-sensitivity of a high-electrochemical-sensitivity of a high-specificity-electrochemical-sensitivity, a high-sensitivity of a nano-specificity-electrochemical sensor, a high-sensitivity of a nano-specificity-sensitivity of a nano-electrochemical-element, a high-specificity-sensitivity of a high-sensitivity of a nano-specificity, a nano-element, a nano-specificity, a high-sensitivity of a nano-specificity, a high-specificity, a nano-element, a high-sensitivity, a high-specificity, a nano-specificity, a high-sensitivity, a nano-sensitivity, a high-sensitivity, a nano-sensitivity, a high-sensitivity, a nano.
Disclosure of Invention
The invention relates to a preparation method of an electrochemical sensor for detecting T-2 toxin residue.
A preparation method of an electrochemical aptamer sensor for detecting T-2 toxin residue comprises the following steps:
preparing a functionalized carbon nano material by adopting a microwave synthesis method, weighing 3-5 g of urea and 2-4 g of citric acid, adding the urea and the citric acid into 8-12 m L deionized water, heating the solution in a microwave oven under the power of 500-1000W for 5-10 min, centrifuging the aqueous solution of the functionalized carbon nano material for 10-20 min at the rotating speed of 2000-4000 rpm, freeze-drying to obtain functionalized carbon nano material powder, preparing the sulfur-doped metal nano sheet/carbon nano material composite material by adopting a hydrothermal synthesis method, dissolving 0.5-1 g of metal salt in 40-60 m L glycol solution, carrying out ultrasonic treatment for 30-50 min at room temperature, then adding 1-1.2 g of thiourea, adding 0.05-0.1 g of the functionalized carbon nano material, carrying out ultrasonic treatment for 40-60 min, transferring the mixed solution into a stainless steel autoclave, carrying out thermal reaction for 10-15 h at the temperature of 150-200 ℃, and drying for 10-14 h at the temperature of 60-80 ℃ to obtain the sulfur-doped metal nano sheet/functionalized carbon nano material;
the signal label is characterized in that the sum of affixes of the signal labels is that 100 mu L3-5 mu M of signal label DNA-S1The strand was added with the same concentration of signal tag DNA-S of 100. mu. L2Then incubating the mixed solution at 37-40 ℃ for 2-4 h, conjugating the T-2 toxin aptamer and the auxiliary DNA, adding the T-2 toxin aptamer of 100 mu L3-5 mu M into the auxiliary DNA of 100 mu L with the same concentration, incubating the mixed solution at 37-40 ℃ for 2-4 h, adding the target substances of 20-40 mu L with different concentrations into the DNA-S of 20-40 mu L1And DNA-S2Placing the mixed solution in a temperature of 37-40 ℃ for incubation for 2-4 h to obtain a conjugated signal label;
the sulfur-doped metal nanosheet/functionalized carbon nanocomposite/aptamer/gold electrode is constructed by placing a gold electrode in a piranha solution, soaking for 20-30 min, washing with ultrapure water, polishing with alumina powder, sequentially placing the polished gold electrode in ethanol and ultrapure water for ultrasonic treatment for 5-10 min, performing electrochemical activation with 0.5-1M of acid solution, dropwise adding 3-5 mu L2-4 mg/M L sulfur-doped metal nanosheet/functionalized carbon nanocomposite to the surface of the electrode, drying under an infrared lamp for 10-20 min, electrodepositing gold for 10-20 s by using chloroauric acid solution, and transferring 3-5 mu L2-4 mu M DNA-H-gold electrode1Dropwise adding the mixture to the surface of an electrode, incubating for 2-4 h at 37-40 ℃, washing and drying the mixture by using a buffer solution, dropwise adding 5-8 mu L0.1-0.4 mM MCH sealant, washing and drying the mixture by using the buffer solution, and dropwise adding 3-5 muL contains a mixture of the target substance and 3-5 mu L DNA-H2And incubating for 2-4 h at 37-40 ℃, dropwise adding a signal label conjugated with 3-5 mu L, incubating for 2-4 h at 37-40 ℃, washing with distilled water and drying to obtain the sulfur-doped metal nanosheet/functionalized carbon nanocomposite/aptamer/gold electrode.
The electrochemical sensor is an electrochemical aptamer sensor for detecting T-2 toxin residues, which is obtained by using a sulfur-doped metal nanosheet/functionalized carbon nanocomposite/aptamer/gold electrode as a working electrode, a platinum wire electrode as a counter electrode and a saturated calomel electrode as a reference electrode and responding to the change of signals through signal molecules.
The metal salt is one or more of sodium ferrite, sodium molybdate, sodium hexanitrocobaltate and copper chloride.
The carbon nano material is one or more of graphene, carbon quantum dots, graphene quantum dots and single-walled carbon nanotubes.
The detergent is a mixed solution of water and absolute ethyl alcohol, and the volume ratio of the detergent to the absolute ethyl alcohol is 1: 1-1: 3.
The signal label is one or more of methylene blue, ferrocene and thionine.
The buffer solution is one or more of phosphate buffer solution, tris buffer solution and 3-morpholine propanesulfonic acid buffer solution.
The acid solution is one or more of sulfuric acid, nitric acid and phosphoric acid.
In the sensor, the functionalized carbon quantum dot/molybdenum disulfide composite material is used as an electrode surface substrate material, the aptamer is used as an identification element, and compared with other T-2 toxin residue detection sensors, the prepared novel electrochemical sensor has the advantages of high response speed, low detection limit, high sensitivity, good repeatability and high accuracy.
Detailed Description
The invention is described below with reference to specific examples:
example 1
The method comprises the following specific steps:
(1) preparing a sulfur-doped molybdenum nanosheet/functionalized graphene quantum dot composite material by adopting a microwave synthesis method, namely preparing functionalized graphene quantum dots by adopting a microwave synthesis method, weighing 3 g of urea and 2 g of citric acid, adding the urea and 2 g of citric acid into 8 m L deionized water, heating the solution in a microwave oven under the power of 500W for 5 min, centrifuging the aqueous solution of the functionalized graphene quantum dots for 10min at the rotating speed of 2000 rpm, and freeze-drying to obtain functionalized graphene quantum dot powder;
(2) signal tag affixation of 100. mu. L3. mu.M signal tag DNA-S1The strand was added with the same concentration of signal tag DNA-S of 100. mu. L2Then incubating the mixed solution at 37 ℃ for 2 h, conjugating the T-2 toxin aptamer and the auxiliary DNA, adding the T-2 toxin aptamer of 100 mu L3 mu M into the auxiliary DNA of 100 mu L with the same concentration, incubating the mixed solution at 37 ℃ for 2-4 h, adding the target of 20 mu L with different concentrations into the DNA-S of 20 mu L1And DNA-S2Placing the mixed solution in a temperature of 37 ℃ for incubation for 2 h to obtain a conjugated signal label;
(3) the sulfur-doped molybdenum nanosheet/functionalized graphene quantum dot composite material/aptamer/gold electrode is constructed by placing a gold electrode in a piranha solution, soaking for 20min, washing with ultrapure water, polishing with alumina powder, sequentially placing the polished gold electrode in ethanol and ultrapure water for ultrasonic treatment for 5 min, performing electrochemical activation with 0.5M acid solution, dropwise adding 3 mu L2 mg/M L of the sulfur-doped molybdenum nanosheet/functionalized graphene quantum dot composite material to the surface of the electrode, drying under an infrared lamp for 10min, electrodepositing gold for 10 s by using chloroauric acid solution, and transferring 3 mu L2 mu M DNA-H1Dropwise adding to the electrode surface, incubating at 37 deg.C for 2 h, washing with buffer solution, air drying, adding 5 μ L0.1 mM MCH blocking agent dropwise, and bufferingAfter the solution is washed and dried, 3 mu L mixed solution containing the target substance and 3 mu L DNA-H are added dropwise2Incubating at 37 ℃ for 2 h, dropwise adding a signal label conjugated with 3 mu L, incubating at 37 ℃ for 2 h, washing with distilled water and drying in the air to obtain a sulfur-doped molybdenum nanosheet/functionalized graphene quantum dot composite material/aptamer/gold electrode;
(4) the electrochemical sensor is an electrochemical aptamer sensor for detecting T-2 toxin residues, which is obtained by using a sulfur-doped molybdenum nanosheet/functionalized graphene quantum dot composite material/aptamer/gold electrode as a working electrode, a platinum wire electrode as a counter electrode and a saturated calomel electrode as a reference electrode and responding to the change of signals through signal molecules.
Example 2
The method comprises the following specific steps:
(1) preparing a sulfur-doped cobalt nanosheet/functionalized carbon quantum dot composite material, namely preparing a functionalized carbon quantum dot by adopting a microwave synthesis method, weighing 5 g of urea and 4 g of citric acid, adding the urea and 4 g of citric acid into 12 m L deionized water, heating the solution in a microwave oven under 1000W of power for 10min, centrifuging the aqueous solution of the functionalized carbon quantum dot for 20min at the rotating speed of 4000 rpm, freeze-drying to obtain functionalized carbon quantum dot powder, preparing the sulfur-doped cobalt nanosheet/carbon quantum dot composite material by adopting a hydrothermal synthesis method, dissolving 1g of sodium hexanitrocobaltate in 60 m L ethylene glycol solution, carrying out ultrasonic treatment for 50 min at room temperature, then adding 1.2 g of thiourea, adding 0.1 g of functionalized carbon quantum dot, carrying out ultrasonic treatment for 60 min, transferring the mixed solution into a stainless steel autoclave, carrying out thermal reaction for 15 h at the temperature of 150-200 ℃, and drying for 14 h at the temperature of 80 ℃ to obtain the sulfur-doped cobalt nanosheet/functionalized carbon quantum dot composite material;
(2) signal tag affixation of 100. mu. L5. mu.M signal tag DNA-S1The strand was added with the same concentration of signal tag DNA-S of 100. mu. L2Then the mixed solution is incubated for 4h at 40 ℃, the T-2 toxin aptamer and the auxiliary DNA are conjugated, 100 mu L5 mu M of the T-2 toxin aptamer is added into the auxiliary DNA with the same concentration of 100 mu L, then the mixed solution is incubated for 4h at 40 ℃, the target with different concentrations of 40 mu L is added into the DNA-S with 40 mu L1And DNA-S2Mixing the solutions, and incubating at 40 deg.C4h, obtaining a signal label of affixation;
(3) the sulfur-doped cobalt nanosheet/functionalized carbon quantum dot composite material/aptamer/gold electrode is constructed by placing a gold electrode in a piranha solution, soaking for 30 min, washing with ultrapure water, polishing with alumina powder, sequentially placing the polished gold electrode in ethanol and ultrapure water for ultrasonic treatment for 10min, performing electrochemical activation with 1M acid solution, dropwise adding 5 mu L4 mg/M L of the sulfur-doped cobalt nanosheet/functionalized carbon quantum dot composite material to the surface of the electrode, drying under an infrared lamp for 20min, electrodepositing gold for 20 s by using chloroauric acid solution, and transferring 5 mu L4 mu M DNA-H1Dropping the mixture onto the surface of electrode, incubating at 40 deg.C for 4 hr, washing with buffer solution, air drying, dropping 8 μ L0.4 mM MCH blocking agent, washing with buffer solution, air drying, dropping 5 μ L mixed solution containing target and 5 μ L DNA-H2Incubating at 40 ℃ for 4h, dropwise adding a signal label conjugated with 5 mu L, incubating at 40 ℃ for 4h, washing with distilled water and drying in the air to obtain the sulfur-doped cobalt nanosheet/functionalized carbon quantum dot composite material/aptamer/gold electrode;
(4) the electrochemical sensor is an electrochemical aptamer sensor for detecting T-2 toxin residues, which is obtained by using a sulfur-doped cobalt nanosheet/functionalized carbon quantum dot composite material/aptamer/gold electrode as a working electrode, a platinum wire electrode as a counter electrode and a saturated calomel electrode as a reference electrode and responding to the change of signals through signal molecules.
The prepared electrochemical sensor has the characteristics of high accuracy, wide linear range (1 fg/m L-100 ng/m L) and low detection lower limit (0.2 fg/m L) on the detection of the T-2 toxin, and meanwhile, the detection result of an actual sample (such as the T-2 toxin in beer and feed) shows that the prepared sensor has very good practical application value.
The above examples are intended to illustrate the invention, but not to limit it. Many modifications and variations of the present invention are possible in light of the above teachings. Within the scope of the appended claims, the invention may be practiced other than as specifically described, and it is within the scope of the claims to select other reagent materials, adjust dispersion times, and the like.
Claims (4)
1. A preparation method of an electrochemical sensor for detecting T-2 toxin residue is characterized by comprising the following steps:
(1) preparing a sulfur-doped metal nano sheet/functionalized carbon nano composite material, namely preparing a functionalized carbon nano material by adopting a microwave synthesis method, weighing 3-5 g of urea and 2-4 g of citric acid, adding the urea and 2-4 g of citric acid into 8-12 m L deionized water, heating the solution in a microwave oven under the power of 500-1000W for 5-10 min, centrifuging the water solution of the functionalized carbon nano material for 10-20 min at the rotating speed of 2000-4000 rpm, and freeze-drying to obtain functionalized carbon nano material powder;
(2) the signal label is added by 100 mu L3-5 mu M of signal label DNA-S1The strand was added with the same concentration of signal tag DNA-S of 100. mu. L2Then incubating the mixed solution at 37-40 ℃ for 2-4 h, conjugating the T-2 toxin aptamer and the auxiliary DNA, adding the T-2 toxin aptamer of 100 mu L3-5 mu M into the auxiliary DNA of 100 mu L with the same concentration, incubating the mixed solution at 37-40 ℃ for 2-4 h, adding the target substances of 20-40 mu L with different concentrations into the DNA-S of 20-40 mu L1And DNA-S2Placing the mixed solution in a temperature of 37-40 ℃ for incubation for 2-4 h to obtain a conjugated signal label;
(3) the sulfur-doped metal nanosheet/functionalized carbon nanocomposite/aptamer/gold electrode is constructed by placing a gold electrode in a piranha solution, soaking for 20-30 min, washing with ultrapure water, polishing with alumina powder, sequentially placing the polished gold electrode in ethanol and ultrapure water for ultrasonic treatment for 5-10 min, performing electrochemical activation with 0.5-1M acid solution, and performing 3-5 mu L2, 2-4 mg/M L sulfur-doped metal nanosheetDripping the functionalized carbon nano composite material on the surface of an electrode, drying for 10-20 min under an infrared lamp, electrodepositing gold for 10-20 s by using chloroauric acid solution, and transferring 3-5 mu L2-4 mu M DNA-H1Dropwise adding the mixture to the surface of an electrode, incubating for 2-4H at 37-40 ℃, washing and drying the mixture with a buffer solution, dropwise adding 5-8 mu L0.1-0.4 mM MCH sealant, washing and drying the buffer solution, and dropwise adding 3-5 mu L of a mixed solution containing a target substance and 3-5 mu L DNA-H2Incubating at 37-40 ℃ for 2-4 h, dropwise adding a signal label conjugated with 3-5 mu L, incubating at 37-40 ℃ for 2-4 h, washing with distilled water and drying in the air to obtain a sulfur-doped metal nanosheet/functionalized carbon nanocomposite/aptamer/gold electrode;
(4) the electrochemical sensor is an electrochemical aptamer sensor for detecting T-2 toxin residues, which is obtained by using a sulfur-doped metal nanosheet/functionalized carbon nanocomposite/aptamer/gold electrode as a working electrode, a platinum wire electrode as a counter electrode and a saturated calomel electrode as a reference electrode and responding to the change of signals through signal molecules.
2. The method for preparing an electrochemical sensor for detecting T-2 toxin residue according to claim 1, wherein in the step (1), the metal salt is one or more of sodium ferrite, sodium molybdate, sodium hexanitrocobaltate and copper chloride; the carbon nano material is one or more of graphene, carbon quantum dots, graphene quantum dots and single-walled carbon nanotubes; the detergent is a mixed solution of water and absolute ethyl alcohol, and the volume ratio of the detergent to the absolute ethyl alcohol is 1: 1-1: 3.
3. The method for preparing an electrochemical sensor for detecting T-2 toxin residues according to claim 1, wherein the signal label in the step (2) is one or more of methylene blue, ferrocene and thionine.
4. The method for preparing an electrochemical sensor for detecting T-2 toxin residues according to claim 1, wherein the buffer solution in the step (3) is one or more of a phosphate buffer solution, a tris buffer solution and a 3-morpholine propanesulfonic acid buffer solution, and the acidic solution is one or more of sulfuric acid, nitric acid and phosphoric acid.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112326950A (en) * | 2020-10-20 | 2021-02-05 | 甘肃农业大学 | Detection method of T-2 toxin |
| CN112456475A (en) * | 2020-11-23 | 2021-03-09 | 湖南医家智烯新材料科技有限公司 | Graphene quantum dot-loaded graphene composite material and preparation method thereof |
| CN112763562A (en) * | 2021-01-28 | 2021-05-07 | 河南工业大学 | Preparation method of branch-shaped walking machine aptamer electrochemical sensor for adenosine triphosphate detection |
| CN116953047A (en) * | 2023-06-29 | 2023-10-27 | 甘肃农业大学 | Current type T-2 aptamer sensor and detection method for T-2 toxin |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104391020A (en) * | 2014-11-04 | 2015-03-04 | 济南大学 | Electrochemical aptamer sensor, preparation method and application thereof |
| CN105004775A (en) * | 2015-07-08 | 2015-10-28 | 青岛大学 | Preparation method of disulfide dot/nanosheet compound DNA electrochemical probe |
| CN105486737A (en) * | 2015-11-28 | 2016-04-13 | 信阳师范学院 | Hollow molybdenum sulfide cubic nanometer electrochemical signal amplifying sensor and its preparation method and use |
| WO2016062101A1 (en) * | 2014-10-20 | 2016-04-28 | 中国人民解放军第三军医大学第一附属医院 | Modified electrode for detecting ndm-1 and preparation method therefor and use thereof |
| CN105784796A (en) * | 2016-03-03 | 2016-07-20 | 青岛大学 | Sensitive lysozyme measuring method of aptasensor based on gold/molybdenum disulfide/graphene nanocomposite |
| CN108760852A (en) * | 2018-04-13 | 2018-11-06 | 江西师范大学 | Photoelectrochemical ochratoxin A detection method based on dual signal amplification |
| CN110108766A (en) * | 2019-01-22 | 2019-08-09 | 重庆医科大学 | A kind of aptamers biosensor preparation method for T-2 Mycotoxin identification in grain or feed |
| CN110618185A (en) * | 2019-08-28 | 2019-12-27 | 江苏大学 | Ratiometric electrochemical detection method of ochratoxin A |
| CN111122677A (en) * | 2020-01-07 | 2020-05-08 | 安徽科技学院 | Electrochemical aptamer sensor for quantitatively detecting ochratoxin A and application thereof |
-
2020
- 2020-05-18 CN CN202010419184.0A patent/CN111398391B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016062101A1 (en) * | 2014-10-20 | 2016-04-28 | 中国人民解放军第三军医大学第一附属医院 | Modified electrode for detecting ndm-1 and preparation method therefor and use thereof |
| CN104391020A (en) * | 2014-11-04 | 2015-03-04 | 济南大学 | Electrochemical aptamer sensor, preparation method and application thereof |
| CN105004775A (en) * | 2015-07-08 | 2015-10-28 | 青岛大学 | Preparation method of disulfide dot/nanosheet compound DNA electrochemical probe |
| CN105486737A (en) * | 2015-11-28 | 2016-04-13 | 信阳师范学院 | Hollow molybdenum sulfide cubic nanometer electrochemical signal amplifying sensor and its preparation method and use |
| CN105784796A (en) * | 2016-03-03 | 2016-07-20 | 青岛大学 | Sensitive lysozyme measuring method of aptasensor based on gold/molybdenum disulfide/graphene nanocomposite |
| CN108760852A (en) * | 2018-04-13 | 2018-11-06 | 江西师范大学 | Photoelectrochemical ochratoxin A detection method based on dual signal amplification |
| CN110108766A (en) * | 2019-01-22 | 2019-08-09 | 重庆医科大学 | A kind of aptamers biosensor preparation method for T-2 Mycotoxin identification in grain or feed |
| CN110618185A (en) * | 2019-08-28 | 2019-12-27 | 江苏大学 | Ratiometric electrochemical detection method of ochratoxin A |
| CN111122677A (en) * | 2020-01-07 | 2020-05-08 | 安徽科技学院 | Electrochemical aptamer sensor for quantitatively detecting ochratoxin A and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| YUKUN YANG 等: "Electrochemiluminescent graphene quantum dots enhanced by MoS2 as sensing platform: a novel molecularly imprinted electrochemiluminescence sensor for 2-methyl-4-chlorophenoxyacetic acid assay", 《ELECTROCHIMICA ACTA》 * |
| 郭玉晶等: "AuNPs-MoS_2-rGO纳米复合材料的合成及其对水合肼的电化学检测", 《山西大学学报(自然科学版)》 * |
| 骆定法等: "基于层状二硫化钼-石墨烯构建新型17β-雌二醇电化学生物传感器", 《信阳师范学院学报(自然科学版)》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112326950A (en) * | 2020-10-20 | 2021-02-05 | 甘肃农业大学 | Detection method of T-2 toxin |
| CN112456475A (en) * | 2020-11-23 | 2021-03-09 | 湖南医家智烯新材料科技有限公司 | Graphene quantum dot-loaded graphene composite material and preparation method thereof |
| CN112763562A (en) * | 2021-01-28 | 2021-05-07 | 河南工业大学 | Preparation method of branch-shaped walking machine aptamer electrochemical sensor for adenosine triphosphate detection |
| CN116953047A (en) * | 2023-06-29 | 2023-10-27 | 甘肃农业大学 | Current type T-2 aptamer sensor and detection method for T-2 toxin |
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