CN112746037B - Streptomyces castochromogenes strain CPAT-W03 and application thereof - Google Patents
Streptomyces castochromogenes strain CPAT-W03 and application thereof Download PDFInfo
- Publication number
- CN112746037B CN112746037B CN202011493757.0A CN202011493757A CN112746037B CN 112746037 B CN112746037 B CN 112746037B CN 202011493757 A CN202011493757 A CN 202011493757A CN 112746037 B CN112746037 B CN 112746037B
- Authority
- CN
- China
- Prior art keywords
- streptomyces
- strain
- cpat
- application
- bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/28—Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
Abstract
The invention discloses a streptomyces badensis strain CPAT-W03 and application thereof, wherein the streptomyces badensis strain 2 is named in a classification wayStreptomyces badiusAnd the preservation unit: china general microbiological culture Collection center (CGMCC), preservation date: 13/01/2020, accession No.: 19342 said Streptomyces auburn belongs to the kingdom of bacteria, phylum Actinomycetes, class Actinomycetes, order Streptomyces, family Streptomycetaceae, genus Streptomyces. The application is the application of the streptomyces badetanus strain 2 in biological control of the cymbidium sinense stem basal rot.
Description
Technical Field
The invention belongs to the technical field of microbial isolation culture, and particularly relates to application of a streptomyces badius strain CPAT-W03 in biological control of cymbidium sinense stem basal rot.
Background
Molan (C)Cymbidium sinense) Is a terrestrial plant of the orchid genus of the Orchidaceae family, and is mainly distributed in China, india, burma, vietnam and ThailandAnd yuzu, japan. The basal rot of the stem of cymbidium sinense is one of the main diseases of artificial cultivation and industrial production. The disease is common and is an important disease on orchid, which affects the growth and appreciation of orchid. The disease is mainly caused by the base of the stem of the cymbidium sinense. At the early stage of disease, light brown and water stain-like scabs appear on roots, and the roots become brown and rot after expansion, and stem bases die. The pathogenic bacteria of black orchid stalk base rot is Phoma (Phoma arachidicola). Because the root of the stem is damaged, the leaf apex is dry in light time, the leaf is yellow green, the growth potential is poor, and the whole plant dies in heavy time, so that serious economic loss is caused.
Due to the increasing 3R problem and the characteristics of orchid as indoor ornamental flower, chemical control is no longer suitable for the control of stalk rot of orchid, so biological control is considered as a green and ecologically feasible control method, and a biological strain which can control the stalk rot of cymbidium sinense and is harmless to the environment and human beings is necessary to be screened and separated.
Disclosure of Invention
The first purpose of the invention is to provide a streptomyces badensis strain (A), (B)Streptomyces badius) (ii) a The second purpose is to provide the application of the streptomyces badius CPAT-W03 in the biological control of the stalk base rot of cymbidium sinense.
All percentages used in the present invention are volume percentages unless otherwise indicated.
The first purpose of the invention is realized by that the streptomyces castaneae strain CPAT-W03 is classified and named as (A)Streptomyces badius) And the preservation unit: china general microbiological culture Collection center (CGMCC), preservation date: year 2020, month 01, day 13, accession number: 19342 said Streptomyces West belongs to the kingdom of bacteria, phylum Actinomycetes, class Actinomycetes, order Streptomyces, family Streptomycetaceae, genus Streptomyces.
Streptomyces castaneae (A) of the inventionStreptomyces badius) CPAT-W03, deposited at China General Microbiological Culture Collection Center (China General Microbiological Culture Collection Center, abbreviated as: CGMCC. The address is microorganism of western No. 1 Hospital No. 3 of Beijing, chaoyang, and Chinese academy of sciencesInstitute, zip code 100080). The preservation number is CGMCC No:19342.
the strain has a good disease inhibiting effect on cymbidium sinense stem basal rot, and can inhibit the growth of pathogenic bacteria without influencing the normal growth of cymbidium sinense.
The bacterial strain can be observed to be inhibited by the cymbidium sinense stem rot pathogenic bacteria on the 5 th day of the opposite culture method, the inhibition phenomenon is more obvious when the bacterial strain is observed on the 7 th day of the opposite culture, and the inhibition zone reaches 6 mm.
The strain is obtained by the following specific steps:
A. collecting a specimen: collecting Panzhihua cycas coralliform mycorrhiza from the cycas natural preservation area in Panzhihua city of Sichuan province;
B. and (3) isolated culture of the strain: adopting a plate marking method to separate the Panzhihua cycas coralliform mycorrhizal endophytic actinomycetes: washing the soil on the surface of coralline mycorrhiza with distilled water, sucking water, sterilizing with 75% alcohol for 1 min, sterilizing with 0.1% mercuric chloride solution for 5 min, sterilizing with 5% sodium hypochlorite solution for 5 min, and rinsing with sterile water for 3 times. Finally, rinse 3 times with sterile water. Sucking the excessive water by using sterile filter paper, fully grinding the coralline mycorrhiza by using a sterile mortar, dipping a small amount of ground tissue fluid by using a sterile inoculating ring, drawing lines on a Gao's first culture medium plate, and culturing for 7-10 days at 28 ℃. After the bacterial colony is formed, using an aseptic inoculating loop to pick out a typical single bacterial colony, and using a scribing method to purify the bacterial strain in a new Gao's No. one culture medium;
C. and (3) strain preservation: inoculating pure bacterial colony of the biocontrol standby bacterial strain obtained in the experiment to a test tube inclined plane, culturing at 28 ℃ for 10 days, and storing in a refrigerator at 4 ℃. The preservation medium is a Gao's first culture medium.
The solid culture medium comprises the following components in g/L: 20 g of soluble starch, 1 g of potassium nitrate, 0.5 g of dipotassium phosphate, 0.5 g of magnesium sulfate heptahydrate, 0.5 g of sodium chloride, 0.01 g of ferrous sulfate heptahydrate, 0.1 g of potassium dichromate, 20 g of agar and 1L of distilled water, wherein the pH value is 7.4-7.6.
The specimen collection place in the step A is a cycas natural preservation area in Panzhihua city, sichuan province.
The specimen in the step A is collected from the glehnia littoralis coralloid mycorrhiza in the cycas natural preservation area.
And step B, operating under an alcohol lamp, burning an inoculating needle and an inoculating loop on the flame of the alcohol lamp, and operating all separation steps under alcohol and the like in the sterile operating platform.
And B, sterilizing materials required by tests such as an inoculating needle, an inoculating loop, sterile water, tweezers, an empty culture medium and the like in a high-pressure steam sterilizer at 121 ℃ for 30 min.
The separation culture condition of the step B is preferable; and (3) standing and culturing for 7 to 10 days at 28 ℃ in the dark.
The conditions for the purification culture and the preservation in the step C are preferred; standing at 28 deg.C in dark for 10 d, and storing at 4 deg.C.
The streptomyces margarizans strain (F) (A)Streptomyces badius) A method for CPAT-W03 in biological control of stem rot of cymbidium sinense comprises screening of biocontrol strains and determination of biocontrol effects, and specifically comprises the following steps:
A. screening of biocontrol strains: and primarily screening the antagonistic bacteria by using a cross method. The specific operation is as follows: pathogenic bacteria of basal rot of cymbidium sinense (A)Phoma arachidicola) Inoculating 6 mm agar blocks of pathogenic bacteria in the center of an improved PDA culture medium plate for indicating bacteria, inoculating single bacterial colonies obtained by isolated culture at equal distance of 2.5 cm away from the pathogenic bacteria, inoculating 4 bacterial strains in each dish, setting for 5 times of repetition, culturing at constant temperature of 28 ℃ for 5-7 days, observing the size of a bacteriostatic ring, and selecting the bacterial strain with the optimal antagonistic effect;
B. measurement of biocontrol effect: in order to screen out strains with stronger bacteriostatic activity, the strains obtained by primary screening are rescreened by adopting a plate opposite growth method: pathogenic bacteria of basal rot of cymbidium sinense (A)Phoma arachidicola) For indicating bacteria, firstly, punching activated pathogenic bacteria agar blocks by using a 6 mm puncher, placing the pathogenic bacteria agar blocks on a flat plate, then inoculating antagonistic bacteria by adopting a point-to-point method, enabling the distance between the two bacteria to be 2.5 cm, only inoculating the pathogenic bacteria agar blocks in a control group, setting 3 times of repetition, culturing for 5 to 7 days in an incubator at 28 ℃, observing the growth condition of the pathogenic bacteria, measuring a bacteria inhibition zone, calculating a bacteria inhibition rate, and finally screening out the antagonistic bacteria with the best antagonistic effect. Calculating the bacteriostasis rateFormula (II): bacteriostatic ratio (%) = (control pathogen colony diameter-treated pathogen colony diameter)/control pathogen colony diameter × 100%.
The improved PDA solid medium comprises the following components in g/L: 200 g of potato, 20 g of glucose, 20 g of agar, 0.5 g of dipotassium phosphate, 0.5 g of magnesium sulfate heptahydrate, 0.01 g of ferrous sulfate heptahydrate and 1L of distilled water, and the pH is natural.
The second purpose of the invention is realized by that the streptomyces margarizans (A), (B) and (C) isStreptomyces badius) Application of CPAT-W03 in biological control of stem basal rot of cymbidium sinense.
The beneficial effects of the invention include the following aspects.
1. The strain has good effect of preventing and controlling the basal stem rot of cymbidium sinense, and has no influence on the normal growth of cymbidium sinense.
2. The bacterial strain can be observed to inhibit the pathogenic bacteria of the stem rot of cymbidium sinense on the 5 th day of opposite culture, and the inhibition phenomenon is more obvious on the 7 th day of opposite culture.
3. The strain is easy to obtain, short in culture time and beneficial to large-scale production, popularization and application.
Drawings
FIG. 1 shows Streptomyces castaneae strain (S. Fagae) (see example 1)Streptomyces badius) CPAT-W03 is cultured on a Gao's first culture medium for 10 d at 28 ℃ to form a bacterial colony on the front surface and the back surface;
FIG. 2 shows Streptomyces aubergiensis strains by plate-confrontation method in example 1 (Streptomyces badius) CPAT-W03 is applied to the front and the back of an antagonistic effect chart in the biological control of the cymbidium sinense stem basal rot;
FIG. 3 shows pathogenic bacteria of basal rot of cymbidium sinense (Moss)Phoma arachidicola) Pure culture bacterial colony front and back.
Detailed Description
The invention is further illustrated by the following examples, but is not intended to be limited in any way, and any modifications or alterations based on the teachings of the invention are intended to fall within the scope of the invention.
The streptomyces badensis strain provided by the inventionCPAT-W03 classification namedStreptomyces badiusThe preservation unit is as follows: china general microbiological culture Collection center (CGMCC), preservation date: 13/01/2020, accession No.: 19342 said Streptomyces auburn belongs to the kingdom of bacteria, phylum Actinomycetes, class Actinomycetes, order Streptomyces, family Streptomycetaceae, genus Streptomyces.
The application of the streptomyces badionotus strain CPAT-W03 disclosed by the invention is the application of the streptomyces badionotus strain CPAT-W03 in biological control of cymbidium root and stem rot.
The Streptomyces lividans strain (A), (B)Streptomyces badius) CPAT-W03 in biological control of cymbidium sinense stem basal rot comprises the steps of strain activation and propagation and biocontrol effect determination, and specifically comprises the following steps:
A. strain activation and propagation: the streptomyces castanensis strain (A) is preparedStreptomyces badius) CPAT-W03 is inoculated on an activation and propagation culture medium, and is cultured for 7 to 10 days at the temperature of 28 ℃ to obtain an activation and propagation strain;
B. and (3) biocontrol effect determination: pathogenic bacteria of stem rot of cymbidium sinense (A)Phoma arachidicola) And (3) inoculating the strain to an improved PDA culture medium, then inoculating the activated and expanded strain prepared in the step A to the culture medium, culturing for 5-7 d at 28 ℃, and observing the antibacterial effect.
The activation and multiplication culture medium is a Gao's first culture medium.
The biocontrol effect is measured by adopting a flat plate confrontation method.
The invention is further illustrated by the following specific examples:
example 1
Streptomyces marochromogenes strain (c: (a))Streptomyces badius) Obtaining, identifying and preserving CPAT-W03.
(1) Streptomyces castochromosis Strain (A)Streptomyces badius) Obtaining and identifying CPAT-W03.
Streptomyces castaneae strain (of the invention)Streptomyces badius) CPAT-W03 is isolated from the sarcodictyon coralloides mycorrhiza of Panzhihuasu. The sample was collected from the cycas revoluta nature reserve area of Panzhihua city, sichuan province, and stored at 4 ℃ for further use. By usingSeparating Panzhihua cycas coralliform mycorrhizal endophytic actinomycetes by using a plate marking method: washing the soil on the surface of coralline mycorrhiza with distilled water, sucking water, sterilizing with 75% alcohol for 1 min, sterilizing with 0.1% mercuric chloride solution for 5 min, sterilizing with 5% sodium hypochlorite solution for 5 min, and rinsing with sterile water for 3 times. Finally rinse 3 times with sterile water. Sucking off excessive water by using sterile filter paper, fully grinding the coralline mycorrhiza by using a sterile mortar, dipping a small amount of ground tissue fluid by using a sterile inoculating ring, drawing lines on a Gao's first culture medium plate, and culturing for 7-10 days at 28 ℃. After the colonies formed, a typical single colony was picked with a sterile inoculating loop and the strain was streaked in new Gao's No. one medium.
The solid culture medium comprises the following components in g/L: 20 g of soluble starch, 1 g of potassium nitrate, 0.5 g of dipotassium phosphate, 0.5 g of magnesium sulfate heptahydrate, 0.5 g of sodium chloride, 0.01 g of ferrous sulfate heptahydrate, 0.1 g of potassium dichromate, 20 g of agar and 1L of distilled water, wherein the pH value is 7.4-7.6.
For the isolated strain CPAT-W03, the experimental results are recorded as follows through morphological identification and molecular biological identification:
A. morphological characteristics: bacterial colony of the strain CPAT-W03 is dark yellow, is nearly circular to irregular, has a fold protruding on the surface and a villous edge, is in a flour shape at the later stage, is dry and opaque, and has a tawny halo around the bacterial colony;
B. 16S rDNA sequence analysis: 16S rDNA sequence determination is carried out on the strain, the 16S rDNA fragment of the strain is subjected to PCR amplification by using the total DNA of the strain CPAT-W03 as a template and an actinomycete 16S rDNA universal primer 8-27F (5': 2 × PowerTaq PCR MasterMix:12.5 μ L, template DNA: 1.μ L, upstream primer (10 μmol/L): 1.μ L, downstream primer (10 μmol/L): 1. mu L, ddH 2 O:9.5 μ L, total volume: 25. and mu L. PCR reaction procedure: after pre-denaturation at 95 ℃ for 5 min, the following cycles of denaturation at 95 ℃ for 1 min, annealing at 55 ℃ for 1 min, extension at 72 ℃ for 2 min,35 cycles of extension at 72 ℃ for 10 min, and finally storage at 4 ℃ in a PCR instrument.
The PCR product is purified by Kunming Shuichi Biotech limitedSequencing, and submitting the sequence to EzBioCloud (https:// www. EzBioCloud. Net /) for comparison after sequence splicing, wherein the comparison result shows that the strain CPAT-W03 and the streptomyces castaneus (CPAT-W03) are compared with each otherStreptomyces badius) (AY 999783) the similarity of the 16S rDNA sequence reached 100%. Identifying the strain CPAT-W03 as Streptomyces castochromogenes by sequence alignment and morphological characteristicsStreptomyces badius). The sequence of the strain CPAT-W03 is shown in a sequence table.
(2) Streptomyces castochromosis Strain (A)Streptomyces badius) Preservation of CPAT-W03.
According to the above identification results, it was confirmed that the strain is Streptomyces chestnuts: (A), (B)Streptomyces badius) The serial number is named as CPAT-W03, and the gene is preserved in China General Microbiological Culture Collection Center (China General Microbiological Culture Collection Center, abbreviated as: CGMCC. The address is as follows: west road No. 1 institute 3, north academy of sciences, china, zip code 100080) in the area of yang. The accession number is 19342.
Example 2
Streptomyces marochromogenes strain (c: (a))Streptomyces badius) And (4) screening the CPAT-W03 strain.
(1) And (4) screening strains.
And primarily screening the antagonistic bacteria by using a cross method. The specific operation is as follows: pathogenic bacteria of basal rot of cymbidium sinense (A)Phoma arachidicola) Inoculating 6 mm agar blocks of pathogenic bacteria in the center of an improved PDA culture medium plate for indicating bacteria, inoculating single bacterial colonies obtained by isolated culture at equal distance of 2.5 cm away from the pathogenic bacteria, inoculating 4 bacterial strains in each dish, setting for 5 times of repetition, culturing at constant temperature of 28 ℃ for 5-7 days, observing the size of a bacteriostatic ring, and selecting the bacterial strain with the optimal antagonistic effect.
(2) And (5) observing the result.
The cross method is adopted to screen the separated strains, and the result shows that 7 strains with antagonistic action on the stalk rot of cymbidium sinense exist.
Example 3
Streptomyces marochromogenes strain (c: (a))Streptomyces badius) CPAT-W03 strain biocontrol effect determination.
(1) And (4) measuring the biocontrol effect.
Confrontation culture method: pathogenic bacteria of basal rot of cymbidium sinense (A)Phoma arachidicola) Firstly, punching an activated pathogenic bacteria agar block by using a 6 mm puncher, placing the activated pathogenic bacteria agar block on a flat plate, then inoculating antagonistic bacteria by adopting a point-to-point method, wherein the distance between the two bacteria is 2.5 cm, only the pathogenic bacteria agar block is inoculated in a control group, setting 3 times of repetition, culturing in an incubator at 28 ℃ for 5 to 7 days, observing the growth condition of the pathogenic bacteria, measuring an inhibition zone, calculating the inhibition rate, and finally screening out the antagonistic bacteria with the best antagonistic effect. The formula for calculating the bacteriostasis rate is as follows: bacteriostatic ratio (%) = (control pathogen colony diameter-treated pathogen colony diameter)/control pathogen colony diameter × 100%.
(2) And (5) observing the result.
The biological control effect of the screened strains is measured by adopting a plate confronting culture method, 7 antagonistic bacteria have antagonistic activity on pathogenic bacteria of the cymbidium base rot, and the inhibition effects of different antagonistic bacteria on pathogenic bacteria hyphae have certain difference. The CPAT-W03 strain has the strongest antagonistic activity and clear bacteriostatic circle boundary, has a bacteriostatic zone of 6 mm on the basal stem rot pathogenic bacteria of cymbidium sinense, and reaches a significant level. Although the culture time is prolonged, the inhibition zone is always transparent, which indicates that the antagonistic bacteria antagonistic performance is stable.
SEQUENCE LISTING
<110> southwest university of forestry
<120> Streptomyces castaneae strain CPAT-W03 and application thereof
<130> 2020
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1287
<212> DNA
<213> 16S rDNA sequence of CPAT-W03 strain
<400> 1
ctgcccttca ctctgggaca agccctggaa acggggtcta ataccggata acactctgtc 60
ccgcatggga cggggttgaa agctccggcg gtgaaggatg agcccgcggc ctatcagctt 120
gttggtgggg taatggccta ccaaggcgac gacgggtagc cggcctgaga gggcgaccgg 180
ccacactggg actgagacac ggcccagact cctacgggag gcagcagtgg ggaatattgc 240
acaatgggcg aaagcctgat gcagcgacgc cgcgtgaggg atgacggcct tcgggttgta 300
aacctctttc agcagggaag aagcgaaagt gacggtacct gcagaagaag cgccggctaa 360
ctacgtgcca gcagccgcgg taatacgtag ggcgcaagcg ttgtccggaa ttattgggcg 420
taaagagctc gtaggcggct tgtcacgtcg gatgtgaaag cccggggctt aaccccgggt 480
ctgcattcga tacgggctag ctagagtgtg gtaggggaga tcggaattcc tggtgtagcg 540
gtgaaatgcg cagatatcag gaggaacacc ggtggcgaag gcggatctct gggccattac 600
tgacgctgag gagcgaaagc gtggggagcg aacaggatta gataccctgg tagtccacgc 660
cgtaaacgtt gggaactagg tgttggcgac attccacgtc gtcggtgccg cagctaacgc 720
attaagttcc ccgcctgggg agtacggccg caaggctaaa actcaaagga attgacgggg 780
gcccgcacaa gcagcggagc atgtggctta attcgacgca acgcgaagaa ccttaccaag 840
gcttgacata taccggaaag catcagagat ggtgcccccc ttgtggtcgg tatacaggtg 900
gtgcatggct gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca 960
acccttgttc tgtgttgcca gcatgccctt cggggtgatg gggactcaca ggagactgcc 1020
ggggtcaact cggaggaagg tggggacgac gtcaagtcat catgcccctt atgtcttggg 1080
ctgcacacgt gctacaatgg ccggtacaat gagctgcgat gccgcgaggc ggagcgaatc 1140
tcaaaaagcc ggtctcagtt cggattgggg tctgcaactc gaccccatga agtcggagtt 1200
gctagtaatc gcagatcagc attgctgcgg tgaatacgtt cccgggcctt gtacacaccg 1260
cccgtcacgt cacgaaagtc ggtaaca 1287
Claims (1)
1. A streptomyces badensis strain CPAT-W03 is characterized in that the streptomyces badensis strain is named by classificationStreptomyces badiusAnd the preservation unit: china general microbiological culture Collection center (CGMCC), preservation date: year 2020, month 01, day 13, accession number: 19342 said Streptomyces auburn strain belongs to the bacteria kingdom, actinomycetes, streptomyces, streptomycetales.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011493757.0A CN112746037B (en) | 2020-12-16 | 2020-12-16 | Streptomyces castochromogenes strain CPAT-W03 and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011493757.0A CN112746037B (en) | 2020-12-16 | 2020-12-16 | Streptomyces castochromogenes strain CPAT-W03 and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112746037A CN112746037A (en) | 2021-05-04 |
| CN112746037B true CN112746037B (en) | 2022-12-13 |
Family
ID=75648489
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011493757.0A Active CN112746037B (en) | 2020-12-16 | 2020-12-16 | Streptomyces castochromogenes strain CPAT-W03 and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112746037B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113046281A (en) * | 2021-05-20 | 2021-06-29 | 福建省农业科学院植物保护研究所 | Streptomyces castochromosis JA58 for preventing and treating potato late blight and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103468599A (en) * | 2013-05-22 | 2013-12-25 | 滨州学院 | Streptomycetes sp. and its application in winter jujube ring rot prevention and treatment |
| CN104293710A (en) * | 2014-09-29 | 2015-01-21 | 中国计量学院 | Streptomycete strains and combined application thereof in prevention and treatment of pepper seedling blight |
| CN104342388A (en) * | 2014-09-29 | 2015-02-11 | 中国计量学院 | Streptomyces strain and combined application thereof in prevention and treatment of tomato blight |
| CN104342389A (en) * | 2014-09-29 | 2015-02-11 | 中国计量学院 | Streptomyces strains and joint application thereof in prevention and treatment of cucumber fusarium wilt |
| CN107354103A (en) * | 2017-04-21 | 2017-11-17 | 浙江师范大学 | Streptomycete Streptomyces lunalinharesii ZJNU968 bacterial strains and its application |
| CN107974427A (en) * | 2018-01-23 | 2018-05-01 | 浙江省柑桔研究所 | One plant of marine streptomyces with bacteriostatic activity |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101781130B (en) * | 2010-02-04 | 2012-09-05 | 大连工业大学 | Method for preparing compost by washing wool junk microbial fermentation |
| CN102199062B (en) * | 2011-03-30 | 2014-04-23 | 北京中天玺龙农业技术发展有限公司 | Biological organic fertilizer for preventing soil salinization of protected land and preparation method thereof |
| KR101695918B1 (en) * | 2015-06-23 | 2017-01-12 | 경상대학교산학협력단 | Streptomyces badius SP6C4 strain having antimicirobial activity against insect pathogen or strawberry fungal disease pathogen isolated from strawberry pollen and uses thereof |
| KR101695916B1 (en) * | 2015-06-24 | 2017-01-12 | 경상대학교산학협력단 | Streptomyces badius SF7B6 strain having antimicrobial activity against pathogen for plant fungal disease isolated from flower of strawberry and uses thereof |
| CN105152809B (en) * | 2015-08-18 | 2018-08-24 | 济南航晨生物科技有限公司 | The technique that complex microorganism decomposes activation Lignitic Humic Acid |
| CN105801311A (en) * | 2016-04-29 | 2016-07-27 | 潍坊英雷生物科技有限公司 | Preparation technology of humic acid compound fertilizer |
| CN106520615A (en) * | 2016-11-16 | 2017-03-22 | 河南农业大学 | Composite microbial system for quickly degrading maize straw and preparation and pretreatment method thereof |
| CN106588417A (en) * | 2016-12-20 | 2017-04-26 | 罗永志 | Special organic fertilizer for hawthorn trees, and preparation method thereof |
| CN107141030A (en) * | 2017-06-06 | 2017-09-08 | 河南科技学院 | A kind of method for improving agricultural organic waste composting efficiency |
| CN107986828A (en) * | 2017-11-28 | 2018-05-04 | 雅安市九龙山农业科技有限公司 | A kind of agricultural organic waste highly effective compost method |
| CN108504604B (en) * | 2018-04-18 | 2021-05-07 | 北京高能时代环境技术股份有限公司 | Compound microbial agent and application thereof in aerobic remediation of landfill |
| CN109439578A (en) * | 2018-11-14 | 2019-03-08 | 合肥华盖生物科技有限公司 | A kind of microbial composite and preparation method thereof for improving water quality |
| CN110423176A (en) * | 2019-08-16 | 2019-11-08 | 吉林农业大学 | A method of promoting dejection organic fertilizer slow release effect |
| CN111484376A (en) * | 2020-04-17 | 2020-08-04 | 山东胜伟盐碱地科技有限公司 | Humic acid bio-organic fertilizer for potted chives and preparation method thereof |
-
2020
- 2020-12-16 CN CN202011493757.0A patent/CN112746037B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103468599A (en) * | 2013-05-22 | 2013-12-25 | 滨州学院 | Streptomycetes sp. and its application in winter jujube ring rot prevention and treatment |
| CN104293710A (en) * | 2014-09-29 | 2015-01-21 | 中国计量学院 | Streptomycete strains and combined application thereof in prevention and treatment of pepper seedling blight |
| CN104342388A (en) * | 2014-09-29 | 2015-02-11 | 中国计量学院 | Streptomyces strain and combined application thereof in prevention and treatment of tomato blight |
| CN104342389A (en) * | 2014-09-29 | 2015-02-11 | 中国计量学院 | Streptomyces strains and joint application thereof in prevention and treatment of cucumber fusarium wilt |
| CN107354103A (en) * | 2017-04-21 | 2017-11-17 | 浙江师范大学 | Streptomycete Streptomyces lunalinharesii ZJNU968 bacterial strains and its application |
| CN107974427A (en) * | 2018-01-23 | 2018-05-01 | 浙江省柑桔研究所 | One plant of marine streptomyces with bacteriostatic activity |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112746037A (en) | 2021-05-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zeng et al. | Use of Coniothyrium minitans and other microorganisms for reducing Sclerotinia sclerotiorum | |
| CN112852638B (en) | Fusarium tobacco root rot high-efficiency biocontrol trichoderma asperellum and application thereof | |
| CN107058160B (en) | One plant of peanut rhizosphere bacillus amyloliquefaciens and its application | |
| CN109762777A (en) | One plant of Paenibacillus polymyxa bacterial strain and its application | |
| CN113736687B (en) | Disease-resistant growth-promoting salt-tolerant bacillus and application thereof | |
| CN104805021A (en) | Cadmium-tolerant penicillium and separation method thereof | |
| CN103642734A (en) | Microbacterium maritypicum and application thereof in preventing sugar beet disease-causing organisms | |
| CN112342174B (en) | Streptomyces castochromogenes strain CPAT-W05 and application thereof | |
| CN112359003B (en) | Western Tang Lianmei bacterial strain and application thereof | |
| CN113444651B (en) | Saffron endophytic fungus and application thereof in preventing and treating bulb rot | |
| CN112746037B (en) | Streptomyces castochromogenes strain CPAT-W03 and application thereof | |
| CN110438017A (en) | Branch spore Saksenaea vasiformis bacterial strain LJ1 and its application | |
| CN112358994B (en) | Pseudomonas and application thereof | |
| CN110317747A (en) | A kind of bacillus amyloliquefaciens JT68 and its application in prevention and treatment tea anthracnose | |
| CN109536390B (en) | Citrus endophytic fungi and application thereof | |
| CN117106639A (en) | Streptomyces nojirimensis strain and application of fermentation liquor thereof in preventing and controlling pepper anthracnose | |
| CN112746038B (en) | Streptomyces castochromogenes strain CPAT-W02 and application thereof | |
| Li et al. | Diversity of cultivable endophytic bacteria associated with halophytes in Xinjiang of China and their plant beneficial traits | |
| CN110527647A (en) | A kind of bacillus and its screening technique and application from the inducible system resistant of tomato rhizosphere | |
| CN116240126A (en) | Multifunctional bacillus belgium SB10 and application thereof | |
| CN114934001A (en) | Endogenous antagonistic bacterium of tobacco seeds and application thereof | |
| CN114276938A (en) | Paecilomyces EJKS strain, fusarium E-9 strain and application thereof | |
| CN107217016A (en) | One plant has the endophytic Bacillus bacterial strain ZY122 for suppressing rice sheath blight disease and its application | |
| CN110982764A (en) | Bacillus tequilensis S12 for preventing and treating rice blast and application thereof | |
| Abdulmugheth et al. | Detection of Salinity Tolerance Pseudomonas Fluorescense Isolates and their Effect on Control Fusarium Wilt Disease Caused by Fusarium Oxysporum F. Sp. Lycopersici on Tomato |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |