CN112725172A

CN112725172A - Kit for detecting macrolide drug-resistant genes of bacteria

Info

Publication number: CN112725172A
Application number: CN202110170164.9A
Authority: CN
Inventors: 屠越兴; 叶祥明; 陈曦; 李曦; 陈敏华; 孙仁华; 励佳妍
Original assignee: Zhejiang Provincial Peoples Hospital
Current assignee: Zhejiang Provincial Peoples Hospital
Priority date: 2021-02-06
Filing date: 2021-02-06
Publication date: 2021-04-30
Anticipated expiration: 2041-02-06
Also published as: CN112725172B

Abstract

The invention discloses a kit for detecting macrolide drug-resistant genes of bacteria, which comprises a kit, wherein a box cover is arranged at the upper end of the kit, the rear end of the box cover is fixedly connected with the rear side of the upper end of the kit through a damping hinge, a lock catch is fixedly connected with the middle position of the upper side of the front end of the kit, the kit and the box cover form an integrated structure through the lock catch, a first groove is arranged on the box cover, the first groove is opened and closed at the middle position of the upper end of the box cover, a handle is arranged in the first groove, the handle is movably connected in the first groove through a screw rod, a temperature controller is fixedly connected at the right side position of the front end of the kit, the rear end of the temperature controller is fixedly connected with the inside of the reagent box, the lower end of the temperature controller is fixedly connected with a control key on the reagent box, the reagent box bottom end fixedly connected with rubber base, the inside left side of kit has seted up the L shape groove. The invention has the advantages of convenient use, simple operation and accurate detection effect.

Description

Kit for detecting macrolide drug-resistant genes of bacteria

Technical Field

The invention relates to the technical field of biology, in particular to a kit for detecting a macrolide drug-resistant gene of bacteria.

Background

After the introduction of western medicine into china, a large number of antibiotics are widely used clinically. However, the inappropriate use of antibiotics has led to an increasing resistance of the bacteria, resulting in a disturbance of the normal flora and the appearance of a large number of resistant strains. Resistant bacteria are able to withstand attack by antibiotic drugs, so that standard treatments lose effectiveness, and infections persist and can infect others. Antibiotic resistance occurs as a result of improper use of antibiotic drugs, and resistance occurs when bacteria mutate or acquire resistance genes. The widespread use of macrolide drugs in the clinic has led to the development of more and more strains resistant to this class of antibiotics, leading to clinical treatment failure. There is a need for a convenient and rapid method for detecting macrolide resistant bacteria, so as to discover drug resistant strains as soon as possible, change medication schemes and guide clinical treatment. Macrolide drugs are stored in a reagent bottle in a static state all the time in the antagonism with antibiotic-resistant strains, so that the detected data are not accurate enough, and therefore, the macrolide drugs are required to be in various environments in the antagonism with antibiotic-resistant strains so as to increase the accuracy of the detected data.

In the prior art, the temperature of macrolide drugs in the resistance to antibiotic-resistant strains changes at any time, so that experimental results are prone to error, data recording is troublesome during detection, detection data in different reagent boxes are prone to be disordered, and damage is prone to occur during carrying to influence the detection results.

Disclosure of Invention

The invention aims to provide a kit for detecting bacterial macrolide drug-resistant genes, which solves the problems that the macrolide drugs proposed in the background art cause error of experimental results due to temperature change at any time during the resistance with antibiotic-resistant strains, are relatively troublesome in data recording during detection, easily cause disorder of detection data in different kits, are easily damaged during transportation, and influence the detection results.

In order to achieve the purpose, the invention provides the following technical scheme: a kit for detecting a bacterial macrolide drug resistance gene comprises a kit, wherein a box cover is arranged at the upper end of the kit, the rear end of the box cover is fixedly connected to the rear side of the upper end of the kit through a damping hinge, a lock catch is fixedly connected to the middle position of the upper side of the front end of the kit, the kit and the box cover form an integrated structure through the lock catch, a first groove is formed in the box cover, the first groove is opened and closed at the middle position of the upper end of the box cover, a handle is arranged in the first groove and movably connected into the first groove through a screw rod, a temperature controller is fixedly connected to the position of the right side of the front end of the kit, the rear end of the temperature controller is fixedly connected into the kit, a control button is fixedly connected to the lower end of the temperature controller on the kit, a rubber base is fixedly connected to the bottom end of, the reagent box comprises an L-shaped groove, a temperature simulation device and a reagent box cover, wherein a rubber base is fixedly connected in the L-shaped groove, the temperature simulation device is fixedly connected in the L-shaped groove and consists of a motor, a connecting shaft and a U-shaped strip, a first rectangular groove is formed in the front end of the right-side reagent box of the L-shaped groove in an opening and closing mode, a second rectangular groove is fixedly connected to the first rectangular groove through screws, a power supply is fixedly connected in the first rectangular groove, a plurality of first rectangular holes are formed in the reagent box at the rear side of the first rectangular groove, a baffle is arranged on the periphery of the first rectangular holes, air holes are formed in the baffle, the air holes extend into the L-shaped groove from left to right, a reagent bearing device is fixedly connected in the first rectangular holes, a second rectangular groove is formed in the right side of the reagent box, a, the collecting drawer is movably connected in the second rectangular hole through a sliding rail, a pull groove is formed in the middle position of the right end of the collecting drawer, a radiating net is formed in the bottom end of the kit, the radiating net is arranged at the lower end of the temperature simulation device and the lower end of the power supply, and a radiating fan is fixedly connected in the radiating net.

Preferably, the four bottom ends of the kit are fixedly connected with four rubber bases respectively, the rubber bases and the kit form an integrated structure, and the bottom ends of the rubber bases are provided with a plurality of anti-skid grooves.

Preferably, the motor is connected with the connecting axle through the pivot, and connecting axle rear end fixedly connected with U-shaped strip, and the motor passes through the axis of rotation and constitutes revolution mechanic with the U-shaped strip, both ends outside is fixed to be seted up fluted two about the U-shaped strip, and two inside difference fixedly connected with refrigeration pieces and the heating strip at both ends about the U-shaped strip.

Preferably, the refrigeration pieces are uniformly and fixedly arranged in the second groove, and the heating strip is horizontally and fixedly connected in the second groove in a transverse mode.

Preferably, the reagent receiving device is composed of a receiving pipe, a spring and protective cotton, the protective cotton is fixedly connected to the inside of the receiving pipe through pasting, and the spring is fixedly connected to the two sides of the receiving pipe.

Preferably, the adapting pipe is formed by vertically dividing a circular pipe into two halves, the shape of the protective sponge is consistent with that of the adapting pipe, the spring is fixedly connected to two ends of the adapting pipe, the other end of the spring is fixedly connected into the first rectangular hole, and the adapting pipe, the spring and the protective cotton form an integrated structure.

Preferably, a partition plate is fixedly connected in the second rectangular groove in the longitudinal direction, a recording plate of the recording device is movably connected to the left side of the second rectangular groove, and a recording pen of the recording device is movably connected to the right side of the second rectangular groove.

Preferably, the motor passes through the wire and connects on the control button, the heating strip passes through the wire and connects on the temperature controller, the refrigeration piece passes through the wire and connects on the temperature controller, the power is connected with on temperature simulation device and the temperature controller through the wire respectively.

Compared with the prior art, the invention has the beneficial effects that:

1. the temperature simulator is favorable for simulating different natural temperatures to test lower drug resistance genes of bacterial macrolides at different temperatures, more detection data are favorable for obtaining, the diversity and comprehensiveness of the detection data are ensured, the reagent carrying device is favorable for ensuring the stability of the reagent bottle, the reagent in the reagent bottle is prevented from being leaked due to large-amplitude shaking when the reagent box is carried, and the temperature controller is favorable for more accurately simulating natural temperatures;

2. the problem of inconvenient recording during detection is solved through the recording device, test recording is conveniently carried out during observation, integrity of each item of data in the later period is guaranteed, omission is avoided, collection of other small parts is facilitated through the collection drawer, the kit is convenient to open and close through the lock catch, heat generated by the temperature simulation device and a power supply is rapidly cooled through the cooling net and the cooling fan, and detection of the kit at normal temperature is guaranteed;

3. through the power, the problem of equipment power supply in the reagent box is solved, be favorable to supplying power for equipment in the reagent box at any time in handling, prevent that the outage from making the testing result have the deviation, adopt the handle, be favorable to making things convenient for the staff to carry the kit, through rubber base and antiskid ribbed tile, stability and reinforcing kit skid resistance can when being favorable to the kit to place.

Drawings

FIG. 1 is a perspective view of the present invention;

FIG. 2 is a schematic view of the inside of a kit according to the present invention;

FIG. 3 is a schematic structural diagram of a temperature simulator according to the present invention;

FIG. 4 is a schematic bottom view of the kit of the present invention;

FIG. 5 is a schematic top view of the interior of the kit of the present invention;

FIG. 6 is a schematic structural view of a rubber mount according to the present invention;

FIG. 7 is a schematic view of the structure of the reagent holding apparatus of the present invention.

In the figure: 1. a kit; 2. a box cover; 3. locking; 4. a first groove; 5. a handle; 6. a temperature controller; 7. a control key; 8. a rubber base; 9. an L-shaped slot; 10. a first groove cover; 11. a temperature simulator; 1101. an electric motor; 1102. a connecting shaft; 1103. a U-shaped strip; 1104. a second groove; 1105. heating the strip; 1106. a refrigeration plate; 12. a first rectangular groove; 13. a second groove cover; 14. a power source; 15. a first rectangular hole; 16. a baffle plate; 17. air holes are formed; 18. a reagent holding device; 1801. a bearing pipe; 1802. a spring; 1803. protective cotton; 19. a second rectangular groove; 20. a recording device; 2001. a recording plate; 2002. a stylus pen; 21. a second rectangular hole; 22. a collection drawer; 23. groove drawing; 24. a heat-dissipating web; 25. a heat radiation fan; 26. an anti-slip groove; 27. a partition plate.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-7, the present invention provides a technical solution: a kit for detecting bacteria macrolide drug resistance genes comprises a kit 1, wherein a box cover 2 is arranged at the upper end of the kit 1, the rear end of the box cover 2 is fixedly connected to the rear side of the upper end of the kit 1 through a damping hinge, a lock catch 3 is fixedly connected to the middle position of the upper side of the front end of the kit 1, the kit 1 and the box cover 2 form an integrated structure through the lock catch 3, a first groove 4 is formed in the box cover 2, the first groove 4 is opened and closed at the middle position of the upper end of the box cover 2, a handle 5 is arranged in the first groove 4, the handle 5 is movably connected into the first groove 4 through a screw rod, a temperature controller 6 is fixedly connected to the right position of the front end of the kit 1, the rear end of the temperature controller 6 is fixedly connected into the kit 1, a control key 7 is fixedly connected to the lower end of the temperature controller 6 on, the upper end of the L-shaped groove 9 is fixedly connected with a groove cover I10 through screws, a rubber base 8 is fixedly connected in the L-shaped groove 9, the L-shaped groove 9 is fixedly connected with a temperature simulation device 11, the temperature simulation device 11 is composed of a motor 1101, a connecting shaft 1102 and a U-shaped strip 1103, the front end in a right side kit 1 of the L-shaped groove 9 is opened and closed with a rectangular groove I12, the rectangular groove I12 is fixedly connected with a groove cover II 13 through screws, a power supply 14 is fixedly connected in the rectangular groove I12, a plurality of rectangular holes I15 are arranged in a rear side kit 1 of the rectangular groove I12, a baffle plate 16 is arranged around the rectangular holes I15, air holes 17 are arranged in the baffle plate 16, the air holes 17 extend into the L-shaped groove 9 from left to right, a reagent bearing device 18 is fixedly connected in the rectangular holes I15, a rectangular groove II 19 is arranged on the right side, the recording device 20 is composed of a recording board 2001 and a recording pen 2002, a second rectangular hole 21 is formed in the right end of the reagent kit 1, a collecting drawer 22 is movably connected in the second rectangular hole 21 through a sliding rail, a pull groove 23 is formed in the middle position of the right end of the collecting drawer 22, a heat dissipation net 24 is formed in the bottom end of the reagent kit 1, the heat dissipation net 24 is arranged at the lower ends of the temperature simulation device 11 and the power supply 14, and a heat dissipation fan 25 is fixedly connected in the heat dissipation net 24.

In the invention: four rubber bases 8 are fixedly connected to the four bottom ends of the kit 1 respectively, the rubber bases 8 and the kit 1 form an integrated structure, and a plurality of anti-slip grooves 26 are formed in the bottom ends of the rubber bases 8, so that the anti-slip performance of the device is enhanced.

In the invention: the motor 1101 is connected with a connecting shaft 1102 through a rotating shaft, the rear end of the connecting shaft 1102 is fixedly connected with a U-shaped strip 1103, the motor 1101 forms a rotating structure with the U-shaped strip 1103 through a rotating shaft, two grooves 1104 are fixedly formed in the outer sides of the left end and the right end of the U-shaped strip 1103, and the two grooves 1104 at the left end and the right end of the U-shaped strip 1103 are fixedly connected with a refrigerating piece 1106 and a heating strip 1105 respectively, so that the simulated temperature detection is facilitated.

In the invention: the refrigeration pieces 1106 are uniformly and fixedly arranged in the second groove 1104, and the heating strip 1105 is horizontally and fixedly connected in the second groove 1104, so that the detection is facilitated by simulating the temperature.

In the invention: the reagent receiving device 18 is composed of a receiving tube 1801, a spring 1802 and a protection cotton 1803, the protection cotton 1803 is fixedly connected to the inside of the receiving tube 1801 through pasting, and the spring 1802 is fixedly connected to two sides of the receiving tube 1801, so that a reagent bottle with a sample can be fixed conveniently.

In the invention: the adapting pipe 1801 is vertically formed by a round pipe in a half-half split mode, the shape of the protective sponge is consistent with that of the adapting pipe 1801, the spring 1802 is fixedly connected to the two ends of the adapting pipe 1801, the other end of the spring 1802 is fixedly connected into the first rectangular hole 15, and the adapting pipe 1801, the spring 1802 and the protective sponge 1803 form an integrated structure, so that a reagent bottle with a sample can be fixed conveniently.

In the invention: the partition plate 27 is longitudinally and fixedly connected in the second rectangular groove 19, the recording plate 2001 of the recording device 20 is movably connected to the left side of the second rectangular groove 19, and the recording pen 2002 of the recording device 20 is movably connected to the right side of the second rectangular groove 19, so that the convenience in recording detection data is facilitated.

In the invention: the motor 1101 is connected to the control key 7 through a lead, the heating strip 1105 is connected to the temperature controller 6 through a lead, the refrigerating sheet 1106 is connected to the temperature controller 6 through a lead, and the power supply 14 is connected to the temperature simulator 11 and the temperature controller 6 through leads respectively, so that the operation of the device is facilitated.

The working principle is as follows: in the detection of macrolide medicines in the bacterial strain confrontation with antibiotic resistance, a box cover 2 is opened through a lock catch 3, a test tube bottle filled with a detection sample is placed into a reagent bearing device 18, a protective cotton 1803 in the reagent bearing device 18 is enabled to be tightly attached to the outer side of the test tube bottle with the sample, a spring 1802 is stressed to fix the test tube bottle with the sample in the reagent bearing device 18, a temperature simulation device 11 is adjusted through a control key 7 to rotate, the temperature is simulated as required, one side of a heating strip 1105 or one side of a refrigerating sheet 1106 on a U-shaped strip 1103 is aligned with a rectangular hole one 15, detection data or a detection result is recorded on a recording device 20, the box cover 2 is fastened, and the reagent box 1 is carried to other places through a handle 5.

In summary, the following steps: through the temperature simulator 11, it is favorable to simulate different natural temperatures to test the drug resistance genes of bacteria macrolides at different temperatures, it is favorable to obtain more detection data, ensure the diversity and comprehensiveness of the detection data, adopt the reagent supporting device 18, it is favorable to ensure the stability of the reagent bottle, prevent from shaking greatly to cause the reagent in the reagent bottle to spill and leak when carrying the reagent box 1, through the temperature controller 6, it is favorable to more accurately simulate the natural temperature conveniently, through the recording device 20, it solves the problem of inconvenient recording during detection, it is favorable to test and record conveniently when observing, it ensures the integrity of each data in the later period, avoid missing, through the collection drawer 22, it is favorable to collect other small parts conveniently, it is convenient to find and use, through the lock catch 3, it is favorable to facilitate the reagent box 1 to open and close conveniently, through radiator-grid 24 and radiator fan 25, be favorable to cooling down the produced heat of temperature simulation device 11 and power 14 fast, guarantee that kit 1 detects under normal temperature, through power 14, the equipment power supply problem in the kit 1 has been solved, be favorable to supplying power for 1 box-interior equipment of kit at any time in the handling, it makes the testing result have the deviation to prevent to cut off the power supply, adopt handle 5, be favorable to making things convenient for the staff to carry kit 1, through to rubber base 8 and antiskid groove 26, stability and reinforcing kit 1 non-slip property when being favorable to kit 1 to place.

The electrical components appearing in the paper, such as the main controller, can be a servo motor, a contact sensor, a processor, an alarm module, a driving module and other conventional known devices for controlling, the standard parts used in the present application document can be purchased from the market, the specific connection mode of each part is connected by conventional means such as bolts, rivets, welding and the like mature in the prior art, the machines, the parts and the devices are of the conventional types in the prior art, and the circuit connection adopts the conventional connection mode in the prior art, so that detailed description is not given here.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A kit for detecting bacterial macrolide drug-resistant genes comprises a kit (1) and is characterized in that: the kit comprises a kit body (1), a box cover (2) is arranged at the upper end of the kit body (1), the rear end of the box cover (2) is fixedly connected to the rear side of the upper end of the kit body (1) through a damping hinge, a lock catch (3) is fixedly connected to the middle position of the upper side of the front end of the kit body (1), the kit body (1) and the box cover (2) form an integrated structure through the lock catch (3), a first groove (4) is formed in the box cover (2), the first groove (4) is opened and closed at the middle position of the upper end of the box cover (2), a handle (5) is arranged in the first groove (4), the handle (5) is movably connected into the first groove (4) through a screw rod, a temperature controller (6) is fixedly connected to the right side of the front end of the kit body (1), the rear end of the temperature controller (6) is fixedly connected to the inside of, the kit comprises a kit body (1), a rubber base (8) is fixedly connected to the bottom end of the kit body (1), an L-shaped groove (9) is formed in the left side of the interior of the kit body (1), a first groove cover (10) is fixedly connected to the upper end of the L-shaped groove (9) through screws, the rubber base (8) is fixedly connected to the interior of the L-shaped groove (9), a temperature simulation device (11) is fixedly connected to the L-shaped groove (9), the temperature simulation device (11) is composed of a motor (1101), a connecting shaft (1102) and a U-shaped strip (1103), a first rectangular groove (12) is formed in the front end of the right side kit body (1) of the L-shaped groove (9) in an opening and closing mode, a second groove cover (13) is fixedly connected to the first rectangular groove (12) through screws, a power source (14) is fixedly connected to the interior of the first rectangular groove (, the kit is characterized in that a baffle (16) is arranged around the first rectangular hole (15), air holes (17) are formed in the baffle (16), the left side and the right side of each air hole (17) extend into the L-shaped groove (9), a reagent bearing device (18) is fixedly connected in the first rectangular hole (15), a second rectangular groove (19) is formed in the right side of the interior of the kit (1), a recording device (20) is fixedly connected in the second rectangular groove (19), the recording device (20) is composed of a recording plate (2001) and a recording pen (2002), the second rectangular hole (21) is formed in the right end of the kit (1), a collecting drawer (22) is movably connected in the second rectangular hole (21) through a sliding rail, a pull groove (23) is formed in the middle position of the right end of the collecting drawer (22), a radiating net (24) is formed in the bottom end of the kit (1), and the radiating net (24) is formed at the lower ends of a temperature simulation, the heat dissipation net (24) is internally and fixedly connected with a heat dissipation fan (25).

2. The kit for detecting a bacterial macrolide drug-resistance gene according to claim 1, wherein: four rubber bases (8) are fixedly connected to the bottom four of the kit (1) respectively, the rubber bases (8) and the kit (1) form an integrated structure, and a plurality of anti-slip grooves (26) are formed in the bottom of each rubber base (8).

3. The kit for detecting a bacterial macrolide drug-resistance gene according to claim 1, wherein: the motor (1101) is connected with a connecting shaft (1102) through a rotating shaft, the rear end of the connecting shaft (1102) is fixedly connected with a U-shaped strip (1103), the motor (1101) forms a rotating structure with the U-shaped strip (1103) through a rotating shaft, two grooves (1104) are fixedly formed in the outer sides of the left end and the right end of the U-shaped strip (1103), and a refrigerating piece (1106) and a heating strip (1105) are fixedly connected with the inner parts of the two grooves (1104) at the left end and the right end of the U-shaped strip (1103) respectively.

4. The kit for detecting a macrolide drug-resistance gene in a bacterium according to claim 3, wherein: the refrigerating pieces (1106) are uniformly and fixedly arranged in the second groove (1104), and the heating strip (1105) is horizontally and fixedly connected in the second groove (1104) in a transverse mode.

5. The kit for detecting a bacterial macrolide drug-resistance gene according to claim 1, wherein: the reagent bearing device (18) is composed of a bearing pipe (1801), springs (1802) and protective cotton (1803), the protective cotton (1803) is fixedly connected to the inside of the bearing pipe (1801) through pasting, and the springs (1802) are fixedly connected to the two sides of the bearing pipe (1801).

6. The kit for detecting a macrolide drug-resistance gene in a bacterium according to claim 5, wherein: the bearing pipe (1801) is composed of two semicircular pipes, the shape of the protective sponge is consistent with that of the bearing pipe (1801), the spring (1802) is fixedly connected to the two ends of the bearing pipe (1801), the other end of the spring (1802) is fixedly connected into the first rectangular hole (15), and the bearing pipe (1801), the spring (1802) and the protective sponge (1803) form an integrated structure.

7. The kit for detecting a bacterial macrolide drug-resistance gene according to claim 1, wherein: a partition plate (27) is fixedly connected in the second rectangular groove (19) in the longitudinal direction, a recording plate (2001) of the recording device (20) is movably connected to the left side of the second rectangular groove (19), and a recording pen (2002) of the recording device (20) is movably connected to the right side of the second rectangular groove (19).

8. The kit for detecting a bacterial macrolide drug-resistance gene according to claim 1, wherein: the motor (1101) is connected to the control key (7) through a lead, the heating strip (1105) is connected to the temperature controller (6) through a lead, the refrigeration piece (1106) is connected to the temperature controller (6) through a lead, and the power supply (14) is connected to the temperature simulator (11) and the temperature controller (6) through leads respectively.