CN112723338A - Specific anti-staphylococcus nitrogen-doped carbon quantum dot and preparation method thereof - Google Patents
Specific anti-staphylococcus nitrogen-doped carbon quantum dot and preparation method thereof Download PDFInfo
- Publication number
- CN112723338A CN112723338A CN202110132042.0A CN202110132042A CN112723338A CN 112723338 A CN112723338 A CN 112723338A CN 202110132042 A CN202110132042 A CN 202110132042A CN 112723338 A CN112723338 A CN 112723338A
- Authority
- CN
- China
- Prior art keywords
- nitrogen
- carbon quantum
- staphylococcus
- doped carbon
- quantum dot
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 title claims abstract description 48
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 230000000941 anti-staphylcoccal effect Effects 0.000 title claims abstract description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 24
- 239000008103 glucose Substances 0.000 claims abstract description 24
- RPNUMPOLZDHAAY-UHFFFAOYSA-N Diethylenetriamine Chemical compound NCCNCCN RPNUMPOLZDHAAY-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 11
- 241000191940 Staphylococcus Species 0.000 claims abstract description 10
- 239000000758 substrate Substances 0.000 claims abstract description 8
- 238000006243 chemical reaction Methods 0.000 claims abstract description 7
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims abstract description 5
- 239000000706 filtrate Substances 0.000 claims description 20
- 239000000843 powder Substances 0.000 claims description 20
- 239000000047 product Substances 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 239000008367 deionised water Substances 0.000 claims description 15
- 229910021641 deionized water Inorganic materials 0.000 claims description 15
- 239000012265 solid product Substances 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 11
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 10
- 238000001816 cooling Methods 0.000 claims description 5
- 238000007865 diluting Methods 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 238000000108 ultra-filtration Methods 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- 238000004108 freeze drying Methods 0.000 claims description 2
- FJVNJEAZIWYZGZ-BTVCFUMJSA-N N'-(2-aminoethyl)ethane-1,2-diamine (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal Chemical compound NCCNCCN.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O FJVNJEAZIWYZGZ-BTVCFUMJSA-N 0.000 claims 1
- 239000002131 composite material Substances 0.000 claims 1
- 241000191967 Staphylococcus aureus Species 0.000 abstract description 15
- 241000191963 Staphylococcus epidermidis Species 0.000 abstract description 5
- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 229960003085 meticillin Drugs 0.000 abstract description 3
- 238000003786 synthesis reaction Methods 0.000 abstract description 3
- 208000015181 infectious disease Diseases 0.000 abstract description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 6
- 230000003385 bacteriostatic effect Effects 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 238000003917 TEM image Methods 0.000 description 5
- 230000000844 anti-bacterial effect Effects 0.000 description 5
- 229910052799 carbon Inorganic materials 0.000 description 5
- 239000002086 nanomaterial Substances 0.000 description 5
- 238000000089 atomic force micrograph Methods 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 238000005033 Fourier transform infrared spectroscopy Methods 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 238000002844 melting Methods 0.000 description 3
- 230000008018 melting Effects 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 229940124350 antibacterial drug Drugs 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002096 quantum dot Substances 0.000 description 2
- 238000012827 research and development Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000006161 blood agar Substances 0.000 description 1
- UHYPYGJEEGLRJD-UHFFFAOYSA-N cadmium(2+);selenium(2-) Chemical compound [Se-2].[Cd+2] UHYPYGJEEGLRJD-UHFFFAOYSA-N 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 229910021389 graphene Inorganic materials 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B32/00—Carbon; Compounds thereof
- C01B32/15—Nano-sized carbon materials
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
- C09K11/65—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing carbon
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Nanotechnology (AREA)
- Organic Chemistry (AREA)
- Inorganic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Materials Engineering (AREA)
- Crystallography & Structural Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Biophysics (AREA)
- Optics & Photonics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Manufacturing & Machinery (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Carbon And Carbon Compounds (AREA)
Abstract
The invention provides a specific staphylococcus-resistant nitrogen-doped carbon quantum dot and a preparation method thereof, wherein the specific staphylococcus-resistant nitrogen-doped carbon quantum dot is synthesized by a one-step method by taking glucose and diethylenetriamine as reaction substrates. The preparation method is simple in preparation process, cheap in reaction substrate and easy in synthesis reaction, and the obtained nitrogen-doped carbon quantum dots have specific anti-staphylococcus activity and can be used for treating the infection wound surfaces of staphylococcus (including staphylococcus aureus, staphylococcus epidermidis and methicillin-resistant staphylococcus aureus (MRSA)).
Description
Technical Field
The invention belongs to the technical field of carbon nano material antibiosis, and particularly relates to a preparation method of a specific staphylococcus-resistant nitrogen-doped carbon quantum dot.
Background
The carbon quantum dot is a novel carbon nano material with the particle size less than 10 nanometers, and is mainly synthesized by taking an organic matter as a precursor substrate through a 'bottom-up' strategy. Among many carbon nanomaterials, the carbon quantum dots belonging to the zero-dimensional carbon nanomaterial have unique morphology, size, surface functional groups and physicochemical characteristics, other elements (such as nitrogen, oxygen and sulfur) are easily doped in the preparation process, and the carbon quantum dots with different properties can be conveniently designed and prepared according to requirements. Compared with other carbon nanomaterials (such as graphene) and semiconductor quantum dots (such as CdSe quantum dots), the carbon quantum dots have obvious advantages in the aspects of heteroelement doping, surface modification and water solubility, generally have better biocompatibility, can interact with biological systems, and exhibit specific biological functions, so that the application of the carbon quantum dots in the field of biomedicine gradually receives wide attention.
In the face of the dilemma of research and development of novel antibacterial drugs, domestic and foreign scholars find that carbon quantum dots prepared under specific conditions by taking specific organic matters as reaction substrates have certain antibacterial activity, and the carbon quantum dots can have the specific antibacterial activity by changing doped elements and the content thereof, open a new research direction for research and development of novel antibacterial drugs, and provide more choices for solving the problem that the drug resistance of bacteria is more and more serious.
Disclosure of Invention
The invention aims to provide a preparation method of a specific anti-staphylococcus nitrogen-doped carbon quantum dot, which has the advantages of simple preparation process, cheap reaction substrate and easy synthesis reaction, and the obtained nitrogen-doped carbon quantum dot has specific anti-staphylococcus activity and can be used for treating the infection wound surface of staphylococcus (including staphylococcus aureus, staphylococcus epidermidis and methicillin-resistant staphylococcus aureus (MRSA)).
In order to achieve the purpose, the invention adopts the following technical scheme:
a preparation method of a specific anti-staphylococcus nitrogen-doped carbon quantum dot comprises the following steps: glucose and diethylenetriamine are used as reaction substrates to synthesize the nitrogen-doped carbon quantum dot with specificity to resist staphylococcus by a one-step method.
The method specifically comprises the following steps:
(1) accurately weighing 2 g of glucose powder, placing the glucose powder in a 50 mL round-bottom flask, and heating for 20 min at the temperature of 150-;
(2) after the glucose powder is melted, adding 2 mL of diethylenetriamine, and continuing to magnetically stir and react for 20 min at the temperature of 150-;
(3) cooling the black solid product to room temperature, and adding 2 mL of deionized water to dissolve the black solid product to obtain a product solution;
(4) then, adjusting the pH value of the product solution to 7 by using 10 mmol/L hydrochloric acid, diluting the product solution to 4 mL by using deionized water, and filtering the product solution by using a 0.22 mu m filter membrane to obtain primary filtrate;
(5) centrifuging the primary filtrate in an ultrafiltration centrifuge tube (3000 MWCO) at 7500 rpm for 10-30 min, discarding the filtrate, adding 2 mL deionized water, mixing, centrifuging at 7500 rpm for 10-30 min, discarding the filtrate, repeating the centrifuging operation for 3 times, and collecting the upper layer liquid;
(6) and after centrifugal treatment, taking the upper layer liquid for freeze drying for 24 hours to obtain the nitrogen-doped carbon quantum dot powder.
Compared with the prior art, the invention has the following advantages and effects:
(1) according to the invention, the nitrogen-doped carbon quantum dots with the particle size of 2-5 nm are successfully prepared by a one-step method by using glucose and diethylenetriamine as reaction substrates, the synthesis process is simple, and the preparation cost is low;
(2) the nitrogen-doped carbon quantum dots have selective antibacterial activity on staphylococcus (including staphylococcus aureus (ATCC 6538), staphylococcus aureus (ATCC 43300), staphylococcus epidermidis and MRSA), and can generate specific antibacterial action on the staphylococcus.
Drawings
FIG. 1 is a Transmission Electron Microscope (TEM) image of nitrogen-doped carbon quantum dots obtained in example 1 of the present invention.
FIG. 2 is an Atomic Force Microscope (AFM) image of nitrogen-doped carbon quantum dots obtained in example 1 of the present invention.
Fig. 3 is a height diagram of the nitrogen-doped carbon quantum dots obtained in example 1 of the present invention in an AFM image.
FIG. 4 is a Fourier Transform Infrared (FTIR) spectrum of nitrogen-doped carbon quantum dots obtained in example 1 of the present invention.
FIG. 5 shows the bacteriostatic effect of the nitrogen-doped carbon quantum dots on Staphylococcus aureus (ATCC 6538) according to the present invention.
FIG. 6 shows the bacteriostatic effect of the nitrogen-doped carbon quantum dots on Staphylococcus aureus (ATCC 3300) according to the present invention.
FIG. 7 shows the bacteriostatic effect of the nitrogen-doped carbon quantum dots on Staphylococcus epidermidis.
FIG. 8 shows the bacteriostatic effect of the nitrogen-doped carbon quantum dots on methicillin-resistant Staphylococcus aureus (MRSA) according to the invention.
FIG. 9 TEM image of Staphylococcus aureus in normal medium.
FIG. 10 is a TEM image of Staphylococcus aureus in the culture medium containing nitrogen-doped carbon quantum dots obtained in example 1 of the present invention.
FIG. 11 TEM image of MRSA in normal medium.
FIG. 12 TEM image of MRSA in a medium containing nitrogen-doped carbon quantum dots obtained in example 1 of the present invention.
Detailed Description
In order to make the content of the present invention more comprehensible, the technical solutions of the present invention are further described below with reference to specific embodiments, but the present invention is not limited thereto.
Example 1
(1) Accurately weighing 2 g of glucose powder, placing the glucose powder in a 50 mL round-bottom flask, and heating for 20 min at 150 ℃ for melting to obtain glucose in a molten state;
(2) after the glucose powder is melted, adding 2 mL of diethylenetriamine, and continuing to magnetically stir and react for 20 min at the temperature of 150 ℃ to obtain a black solid product;
(3) cooling the black solid product to room temperature, and adding 2 mL of deionized water to dissolve the black solid product to obtain a product solution;
(4) then, adjusting the pH value of the product solution to 7 by using 10 mmol/L hydrochloric acid, diluting the product solution to 4 mL by using deionized water, and filtering the product solution by using a 0.22 mu m filter membrane to obtain primary filtrate;
(5) centrifuging the primary filtrate in an ultrafiltration centrifuge tube (3000 MWCO) at 7500 rpm for 30 min, discarding the filtrate, adding 2 mL deionized water, mixing, centrifuging at 7500 rpm for 30 min, discarding the filtrate, repeating the centrifuging operation for 3 times, and collecting the upper layer liquid;
(6) after the centrifugal treatment, the upper layer liquid was taken out and freeze-dried for 24 h to obtain nitrogen-doped carbon quantum dot powder, which was recorded as M1.
Fig. 1, fig. 2 and fig. 3 are a TEM image, an AFM image and a height sectional view of the nitrogen-doped carbon quantum dot in the AFM image, respectively, and it can be seen from the images that the nitrogen-doped carbon quantum dot obtained by the present invention has better dispersibility and uniformity and a particle size of about 5 nm. FIG. 4 is an FTIR chart of nitrogen-doped carbon quantum dots, from which it can be seen that the surface of the nitrogen-doped carbon quantum dots obtained by the present invention contains amino groups (-NH-or-NH-)2) Carboxyl (-COOH) and hydroxyl (-OH).
Example 2
(1) Accurately weighing 2 g of glucose powder, placing the glucose powder in a 50 mL round-bottom flask, and heating for 20 min at 155 ℃ for melting to obtain glucose in a molten state;
(2) after the glucose powder is melted, adding 2 mL of diethylenetriamine, and continuing to magnetically stir and react for 20 min at the temperature of 155 ℃ to obtain a black solid product;
(3) cooling the black solid product to room temperature, and adding 2 mL of deionized water to dissolve the black solid product to obtain a product solution;
(4) then, adjusting the pH value of the product solution to 7 by using 10 mmol/L hydrochloric acid, diluting the product solution to 4 mL by using deionized water, and filtering the product solution by using a 0.22 mu m filter membrane to obtain primary filtrate;
(5) centrifuging the primary filtrate in an ultrafiltration centrifuge tube (3000 MWCO) at 7500 rpm for 30 min, discarding the filtrate, adding 2 mL deionized water, mixing, centrifuging at 7500 rpm for 30 min, discarding the filtrate, repeating the centrifuging operation for 3 times, and collecting the upper layer liquid;
(6) after the centrifugal treatment, the upper layer liquid was taken out and freeze-dried for 24 h to obtain nitrogen-doped carbon quantum dot powder, which was recorded as M2.
Example 3
(1) Accurately weighing 2 g of glucose powder, placing the glucose powder in a 50 mL round-bottom flask, and heating for 20 min at 160 ℃ for melting to obtain glucose in a molten state;
(2) after the glucose powder is melted, adding 2 mL of diethylenetriamine, and continuing to magnetically stir and react for 20 min at the temperature of 160 ℃ to obtain a black solid product;
(3) cooling the black solid product to room temperature, and adding 2 mL of deionized water to dissolve the black solid product to obtain a product solution;
(4) then, adjusting the pH value of the product solution to 7 by using 10 mmol/L hydrochloric acid, diluting the product solution to 4 mL by using deionized water, and filtering the product solution by using a 0.22 mu m filter membrane to obtain primary filtrate;
(5) centrifuging the primary filtrate in an ultrafiltration centrifuge tube (3000 MWCO) at 7500 rpm for 30 min, discarding the filtrate, adding 2 mL deionized water, mixing, centrifuging at 7500 rpm for 30 min, discarding the filtrate, repeating the centrifuging operation for 3 times, and collecting the upper layer liquid;
(6) after the centrifugal treatment, the upper layer liquid was taken out and freeze-dried for 24 h to obtain nitrogen-doped carbon quantum dot powder, which was recorded as M3.
Application examples
Bacterial culture and bacterial liquid preparation method
All test bacteria are firstly inoculated on a blood agar plate by a plate marking method and cultured in a biochemical incubator (35 ℃) to obtain a single colony, the single colony is picked by an inoculating loop and inoculated in sterilized normal saline (0.9 percent sodium chloride solution) to obtain a bacterial suspension, and the OD600 value of the bacterial suspension is measured and adjusted to be 0.1 by an ultraviolet visible spectrophotometer to obtain 1.5 multiplied by 108CFU/mL of bacterial suspension.
Paper sheet diffusion experiment method
Using sterile medical cotton swab to make concentration be 1.5X 108CFU/mL bacterial suspension is evenly smeared on MH agar culture medium (phi =90 mm), and then drug sensitive paper sheets (phi =6 mm) containing test nitrogen-doped carbon quantum dots are placed, wherein the distance between the centers of each drug sensitive paper sheet is at least 24 mm. After incubation for 18 hours in a biochemical incubator (35 ℃), photographs were taken and the diameter of the zone of inhibition around each drug sensitive paper piece was measured with a vernier caliper.
Fig. 5, 6, 7 and 8 are paper diffusion experiments of the nitrogen-doped carbon quantum dots obtained in examples 1-3 on staphylococcus aureus (ATCC 6538), staphylococcus aureus (ATCC 3300), staphylococcus epidermidis and MRSA, respectively, and it can be seen from the drawings that the nitrogen-doped carbon quantum dots obtained in the present invention have good bacteriostatic effects on the above four bacteria, form significant bacteriostatic rings on MH agar plates, and have diameters larger than 15 mm. FIGS. 9 and 11 show Staphylococcus aureus and MRSA cultured under normal conditions, and it can be seen that both bacteria have intact cell structures. FIGS. 10 and 12 show Staphylococcus aureus and MRSA incubated in the culture medium containing the nitrogen-doped carbon quantum dots, from which it can be clearly seen that the cell walls and cell membranes of the two bacteria are obviously broken after being acted by the nitrogen-doped carbon quantum dots, the thallus is disintegrated, the intracellular substances are leaked, and the thallus cells lose integrity. The result proves that the obtained nitrogen-doped carbon quantum dots have good antibacterial effect on staphylococcus.
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.
Claims (3)
1. The utility model provides a nitrogen-doped carbon quantum dot of anti staphylococcus of specificity which characterized in that: the glucose-diethylenetriamine composite material comprises glucose and diethylenetriamine, wherein the proportion of the glucose to the diethylenetriamine is as follows: 1 g: 1 mL.
2. A preparation method of a specific anti-staphylococcus nitrogen-doped carbon quantum dot is characterized by comprising the following steps: glucose and diethylenetriamine are used as reaction substrates to synthesize the nitrogen-doped carbon quantum dot with specificity to resist staphylococcus by a one-step method.
3. The method for preparing the specific anti-staphylococcus nitrogen-doped carbon quantum dot according to claim 2, wherein the method comprises the following steps: the method specifically comprises the following steps:
(1) accurately weighing 2 g of glucose powder, placing the glucose powder in a 50 mL round-bottom flask, and heating for 20 min at the temperature of 150-;
(2) after the glucose powder is melted, adding 2 mL of diethylenetriamine, and continuing to magnetically stir and react for 20 min at the temperature of 150-;
(3) cooling the black solid product to room temperature, and adding 2 mL of deionized water to dissolve the black solid product to obtain a product solution;
(4) then, adjusting the pH value of the product solution to 7 by using 10 mmol/L hydrochloric acid, diluting the product solution to 4 mL by using deionized water, and filtering the product solution by using a 0.22 mu m filter membrane to obtain primary filtrate;
(5) centrifuging the primary filtrate in 3000 MWCO ultrafiltration centrifuge tube at 7500 rpm for 10-30 min, discarding filtrate, adding 2 mL deionized water, mixing, centrifuging at 7500 rpm for 10-30 min, discarding filtrate, repeating centrifuging operation for 3 times, and collecting upper layer liquid;
(6) and after centrifugal treatment, taking the upper layer liquid for freeze drying for 24 hours to obtain the nitrogen-doped carbon quantum dot powder.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110132042.0A CN112723338A (en) | 2021-01-30 | 2021-01-30 | Specific anti-staphylococcus nitrogen-doped carbon quantum dot and preparation method thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110132042.0A CN112723338A (en) | 2021-01-30 | 2021-01-30 | Specific anti-staphylococcus nitrogen-doped carbon quantum dot and preparation method thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
CN112723338A true CN112723338A (en) | 2021-04-30 |
Family
ID=75594949
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202110132042.0A Pending CN112723338A (en) | 2021-01-30 | 2021-01-30 | Specific anti-staphylococcus nitrogen-doped carbon quantum dot and preparation method thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN112723338A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114105121A (en) * | 2021-11-01 | 2022-03-01 | 浙江省农业科学院 | Starch carbon quantum dot and preparation method and application thereof |
CN114477138A (en) * | 2021-12-15 | 2022-05-13 | 浙江工业大学 | Preparation method of potato carbon quantum dots and degradable preservative film with high bacteriostatic activity |
CN115849347A (en) * | 2023-01-19 | 2023-03-28 | 四川大学 | Preparation method and application of arginine antibacterial carbon dots |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108102650A (en) * | 2018-01-29 | 2018-06-01 | 陕西师范大学 | A kind of high-purity pyridine nitrogen atom doping type carbon quantum dot and preparation method thereof |
CN110853936A (en) * | 2019-11-27 | 2020-02-28 | 长安大学 | Preparation method of electrode material |
WO2021008092A1 (en) * | 2019-07-17 | 2021-01-21 | 湖北大学 | Method for preparing near-infrared responsive functional coating on surface of cylindrical titanium nail and application |
-
2021
- 2021-01-30 CN CN202110132042.0A patent/CN112723338A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108102650A (en) * | 2018-01-29 | 2018-06-01 | 陕西师范大学 | A kind of high-purity pyridine nitrogen atom doping type carbon quantum dot and preparation method thereof |
WO2021008092A1 (en) * | 2019-07-17 | 2021-01-21 | 湖北大学 | Method for preparing near-infrared responsive functional coating on surface of cylindrical titanium nail and application |
CN110853936A (en) * | 2019-11-27 | 2020-02-28 | 长安大学 | Preparation method of electrode material |
Non-Patent Citations (2)
Title |
---|
CHENGFEI ZHAO, ET AL.: "Chengfei Zhao, et al.", 《COLLOIDS AND SURFACES B: BIOINTERFACES》 * |
张研等: "荧光碳量子点的制备及其在生物医用领域的研究进展", 《材料导报》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114105121A (en) * | 2021-11-01 | 2022-03-01 | 浙江省农业科学院 | Starch carbon quantum dot and preparation method and application thereof |
CN114477138A (en) * | 2021-12-15 | 2022-05-13 | 浙江工业大学 | Preparation method of potato carbon quantum dots and degradable preservative film with high bacteriostatic activity |
CN114477138B (en) * | 2021-12-15 | 2023-07-07 | 浙江工业大学 | Preparation method of potato carbon quantum dot and degradable preservative film with high antibacterial activity |
CN115849347A (en) * | 2023-01-19 | 2023-03-28 | 四川大学 | Preparation method and application of arginine antibacterial carbon dots |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN112723338A (en) | Specific anti-staphylococcus nitrogen-doped carbon quantum dot and preparation method thereof | |
Ullah et al. | Structural and physico-mechanical characterization of bio-cellulose produced by a cell-free system | |
Cheng et al. | Enhanced production of bacterial cellulose by using a biofilm reactor and its material property analysis | |
Spagnol et al. | Cellulose nanowhiskers decorated with silver nanoparticles as an additive to antibacterial polymers membranes fabricated by electrospinning | |
Herricks et al. | Direct fabrication of enzyme-carrying polymer nanofibers by electrospinning | |
Kannan et al. | Green synthesis of ruthenium oxide nanoparticles: Characterization and its antibacterial activity | |
Bojarska et al. | Growth of ZnO nanowires on polypropylene membrane surface—characterization and reactivity | |
CN112337466B (en) | Nanocarbon-loaded cluster copper nanoenzyme and preparation method and application thereof | |
CN111248224A (en) | Preparation method and antibacterial activity test method of antibacterial agent based on MXene quantum dots | |
CN102924755A (en) | Preparation method of graphene/bacterial cellulose composite material | |
CN110316757B (en) | Preparation method and application of vanadium oxide | |
Shan et al. | Flexible, mesoporous, and monodispersed metallic cobalt-embedded inorganic nanofibrous membranes enable ultra-fast and high-efficiency killing of bacteria | |
Tian et al. | Enhancing bacterial cellulose production via adding mesoporous halloysite nanotubes in the culture medium | |
CN115646486A (en) | Graphene-supported palladium nanoenzyme as well as preparation method and application thereof | |
Zhan et al. | Fabrication, characterization and antibacterial properties of ZnO nanoparticles decorated electrospun polyacrylonitrile nanofibers membranes | |
CN111410809A (en) | Persistent antibacterial polyvinyl alcohol film based on organic metal framework and preparation method thereof | |
Liu et al. | Facile one-pot green synthesis and antibacterial activities of GO/Ag nanocomposites | |
CN107083582B (en) | Silver composite iron oxide fiber and preparation method and application thereof | |
Sharma et al. | Rotary disc bioreactor-based approach for bacterial nanocellulose production using Gluconacetobacter xylinus NCIM 2526 strain | |
Zhu et al. | In situ synthesis of cuprous oxide on cotton fiber for developing functional textile with broad-spectrum antibacterial activity | |
Oskouei et al. | Preparation and characterization of novel antimicrobial Cu-Al layered double hydroxide filled polyvinyl alcohol/chitosan nanofibers with peroxidase-mimic activity | |
RU2767952C1 (en) | Method of producing ferrihydrite nanoparticles | |
CN115354491B (en) | Nanocellulose composite material and preparation method and application thereof | |
CN114410617B (en) | Immobilization method for improving biological hydrogen synthesis of hydrogen-producing bacteria and application | |
CN116814456A (en) | Colt-shaped bacillus and application thereof in synthesizing bacterial cellulose |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210430 |