CN112715195B - Method and device for screening island greening salt-reducing plants - Google Patents

Method and device for screening island greening salt-reducing plants Download PDF

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Publication number
CN112715195B
CN112715195B CN202011401260.1A CN202011401260A CN112715195B CN 112715195 B CN112715195 B CN 112715195B CN 202011401260 A CN202011401260 A CN 202011401260A CN 112715195 B CN112715195 B CN 112715195B
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plant
plants
test
salt
planting
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CN112715195A (en
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陈浩
孙锡斌
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Institute of Subtropical Agriculture of CAS
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Institute of Subtropical Agriculture of CAS
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01BMEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
    • G01B21/00Measuring arrangements or details thereof, where the measuring technique is not covered by the other groups of this subclass, unspecified or not relevant
    • G01B21/02Measuring arrangements or details thereof, where the measuring technique is not covered by the other groups of this subclass, unspecified or not relevant for measuring length, width, or thickness
    • G01B21/08Measuring arrangements or details thereof, where the measuring technique is not covered by the other groups of this subclass, unspecified or not relevant for measuring length, width, or thickness for measuring thickness
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01BMEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
    • G01B21/00Measuring arrangements or details thereof, where the measuring technique is not covered by the other groups of this subclass, unspecified or not relevant
    • G01B21/10Measuring arrangements or details thereof, where the measuring technique is not covered by the other groups of this subclass, unspecified or not relevant for measuring diameters
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/44Sample treatment involving radiation, e.g. heat
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/71Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light thermally excited
    • G01N21/73Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light thermally excited using plasma burners or torches

Abstract

The invention discloses a method and a device for screening island greening salt-reducing plants, which comprises the following steps: selecting a sample prescription, selecting and planting plants and comparing parameters; the perchloric acid digestion device in the parameter comparison step consists of a base, an X/Y/Z axis module and a reagent filling assembly, and the reagent filling assembly is moved through a plurality of axial modules. In the invention, a plurality of groups of planting sample formulas are established on the seashore close to the coastline, three comparison sample formulas are planted for a long time, then different plants are subjected to the method of measuring by adopting a concentrated sulfuric acid-perchloric acid digestion-inductively coupled plasma spectroscope to measure the absorption quantity of sodium, potassium, calcium and magnesium in the plants, and the salt-reducing plants suitable for planting in the seashore area can be obtained by the method.

Description

Method and device for screening island greening salt-reducing plants
Technical Field
The invention relates to the technical field of plant planting, in particular to a method and a device for screening island greening salt-reducing plants.
Background
Soil salinization is a worldwide resource and ecological problem, and according to statistics of grain and agriculture organizations and textbook organizations of united nations, various salinized soil is about 10 hundred million hm2 in the world, and is 10% of the global land area, and is widely distributed in more than 100 countries and regions. At the same time, global saline-alkali soil is growing older every year at a rate of 1.0X106-1.5X106 hm2. In China, the saline soil area is 3.47×107hm < 2 >. Coastal zones and island coasts of about 1.8 km in coastal provinces, municipalities and autonomous regions in China are widely distributed with various coastal saline soil with total area of 5 multiplied by 106hm, and the coastal saline soil mainly comprises coastal alluvial plains in the north of Yangtze river, hebei, liaoning and other provinces and the north of Jiangsu and partial areas of coastal zones in the south of Yangtze river, fujian, guangdong and other provinces. The high salt content soil in coastal areas can cause a certain negative influence on the environment, and can cause the problems of low land productivity and difficult vegetation establishment, thereby severely restricting the quality and quantity of afforestation in the areas. Meanwhile, the plant materials for greening and forestation of the coastal saline-alkali soil are few, the forest species are single, the tree species are monotonous, and the forestry development of the coastal saline-alkali soil is limited. Therefore, in order to improve the ecological environment and to improve the air quality, it is necessary to cultivate a batch of plants adapted to the environment of high salt content.
Disclosure of Invention
The invention aims to solve the problems and provides a method and a device for screening island greening salt-reducing plants.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a method for screening island greening salt-reducing plants, comprising the steps of:
selecting sample sides, and building nine 15m×9m sample sides on the coastal kelp less than one kilometer from coastline, dividing into three groups, each group comprising three treatment areas of control, low-volume seawater addition and high-volume seawater addition, wherein the low-volume treatment addition seawater is 200mmyr -1 High-volume treatment of added seawater 400mmyr -1 Adding fresh water with the same volume as the low-volume treatment in the control treatment;
selecting and planting plants, wherein twelve common tree species in a tropical coastal region are planted in each sample, including acacia macrophylla, casuarina equisetifolia, mulberry, semen euryales, coco, pseudo-jasmine, ceris Mangifera, jatropha curcas, hibiscus, morinda citrifolia and erythrina ku, spraying seawater through a sprayer every half month after planting, and each sample is irrigated with fresh water in an equivalent amount to ensure that the plant growth is not limited by water;
parameter comparison, after one and a half years of planting, measuring the chest diameter increment, the tree height increment, biomass and salt absorption of the plant, wherein the biomass of the plant is estimated by adopting a different-speed growth model, and the model formula is w=a b Wherein W is biomass, D is plant breast diameter, H is plant height, a and b are model parameters, the salt-reducing amount of the plant is characterized by the absorption amount of sodium, potassium, calcium and magnesium in the plant, and the sodium, potassium, calcium and magnesium content of the plant is measured by adopting a concentrated sulfuric acid-perchloric acid digestion-inductively coupled plasma spectrometer.
As a further description of the above technical solution:
the utility model provides a device of cooking that disappears that perchloric acid used in parameter comparison step, includes base and controller, the top of base has a plurality of recesses and test-tube rack, the inside of recess is equipped with helical heating wire, be equipped with a plurality of spacing subassemblies that are used for the centre gripping test tube on the test-tube rack, be equipped with on the base be used for supporting the first extensible member of test-tube rack, be located the reagent filling subassembly of test-tube rack top and be used for driving the drive module that reagent filling subassembly removed, drive module comprises X axle module, Y axle module and Z axle module, reagent filling subassembly includes:
the movable block is arranged on the Z-axis module, a cavity is formed in the movable block, and a first pipe body and a second pipe body are arranged in the cavity;
the upper cover is connected with the movable block through a bolt, a cover body is arranged at the top of the upper cover, a second telescopic piece and a driving piece are arranged on the inner side of the cover body, a plurality of cylinders are arranged at the bottom of the upper cover, a piston is arranged in the cylinders, a needle head is arranged at the bottom of the cylinders, and the driving piece is connected with the piston through a transmission assembly.
As a further description of the above technical solution:
the heating wire is sleeved with a heat insulation sleeve with an annular structure, and the heat insulation sleeve is made of any one of glass fiber, asbestos and rock wool.
As a further description of the above technical solution:
the limiting assembly comprises a limiting sleeve, a coil spring is arranged on the inner side of the limiting sleeve, and at least three annular protruding blocks which are uniformly distributed are arranged on the inner side of the coil spring.
As a further description of the above technical solution:
the side of test-tube rack is equipped with the fixed cover of cover locating first extensible member flexible end and handle.
As a further description of the above technical solution:
the first pipe body is embedded in the bottom of the movable block, the lower end of the second pipe body is located on the inner side of the first pipe body, and the upper end of the second pipe body is of a funnel-shaped structure.
As a further description of the above technical solution:
the lower extreme of syringe needle extends to the inboard of second body, drive assembly includes the drive gear who is connected with the driving piece output and rotates the thread bush of connection at the barrel top, the inboard of thread bush closes soon and is connected with the threaded rod to the outside cover is equipped with driven gear, the lower extreme and the piston connection of threaded rod, the threaded rod is hollow structure, the bottom of upper cover is equipped with the gag lever post of inlaying and establishing at the threaded rod inside.
As a further description of the above technical solution:
the top of test tube is equipped with the sealing plug, the inside of sealing plug is equipped with a plurality of elasticity separation blade, the outside parcel of elasticity separation blade has elastic rubber.
In summary, due to the adoption of the technical scheme, the beneficial effects of the invention are as follows:
1. in the invention, a plurality of groups of planting sample formulas are established on the seashore close to the coastline, three comparison sample formulas are planted for a long time, then different plants are subjected to the method of measuring by adopting a concentrated sulfuric acid-perchloric acid digestion-inductively coupled plasma spectroscope to measure the absorption quantity of sodium, potassium, calcium and magnesium in the plants, and the salt-reducing plants suitable for planting in the seashore area can be obtained by the method.
2. In the invention, the digestion device consists of the base, the X/Y/Z axis module and the reagent filling assembly, and the reagent filling assembly is moved by the plurality of axial modules, so that the automatic operation is realized, and compared with the conventional manual operation, the digestion device has the advantages of high efficiency and convenience.
Drawings
FIG. 1 is a schematic view of the overall structure of the device of the present invention;
FIG. 2 is a schematic view of the overall structure of the device according to another aspect of the present invention;
FIG. 3 is a schematic view of the internal structure of the groove according to the present invention;
FIG. 4 is a schematic view of the inner side structure of the stop collar according to the present invention;
FIG. 5 is a schematic view of the internal structure of the movable block according to the present invention;
FIG. 6 is a schematic view of the bottom structure of the movable block according to the present invention;
FIG. 7 is a schematic view of the bottom structure of the upper cover of the present invention;
FIG. 8 is a schematic diagram of the front view of the upper cover of the present invention;
FIG. 9 is a schematic view of the internal structure of the sealing plug of the present invention;
FIG. 10 is a bar graph of plant chest diameter increments according to the present invention;
FIG. 11 is a high delta histogram of a plant tree in accordance with the present invention;
FIG. 12 is a bar graph of plant biomass according to the invention;
FIG. 13 is a bar graph of plant salt absorption in accordance with the present invention.
Legend description:
1. a base; 2. a groove; 3. a test tube rack; 4. heating wires; 5. a heat insulating sleeve; 6. a limit sleeve; 7. a coil spring; 8. a bump; 9. a first telescopic member; 10. an X-axis module; 11. a Y-axis module; 12. a Z-axis module; 13. a movable block; 14. an upper cover; 15. a cavity; 16. a first tube body; 17. a second tube body; 18. a cover body; 19. a second telescopic member; 20. a driving member; 21. a drive gear; 22. a cylinder; 23. a thread sleeve; 24. a driven gear; 25. a threaded rod; 26. a piston; 27. a limit rod; 28. a needle; 29. a sealing plug; 30. an elastic baffle.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Examples:
referring to fig. 1-9, a method for screening island greening salt-reducing plants comprises the following steps:
selecting sample sides, and building nine 15m×9m sample sides on the coastal kelp less than one kilometer from coastline, dividing into three groups, each group comprising three treatment areas of control, low-volume seawater addition and high-volume seawater addition, wherein the low-volume treatment addition seawater is 200mmyr -1 Approximately corresponds to the local annual drop10% of rainfall, and high-volume treatment is carried out to add 400mmyr of sea water -1 Approximately corresponding to 20% of the rainfall in the local year, and the fresh water with the same volume as the low-volume treatment is added in the control treatment;
selecting and planting plants, wherein twelve common tree species in a tropical coastal region are planted in each sample, including acacia macrophylla, casuarina equisetifolia, mulberry, semen euryales, coco, pseudo-jasmine, ceris Mangifera, jatropha curcas, hibiscus, morinda citrifolia and erythrina ku, spraying seawater through a sprayer every half month after planting, and each sample is irrigated with fresh water in an equivalent amount to ensure that the plant growth is not limited by water;
parameter comparison, after one and a half years of planting, measuring the chest diameter increment, the tree height increment, biomass and salt absorption of the plant, wherein the biomass of the plant is estimated by adopting a different-speed growth model, and the model formula is w=ad 2 H b Wherein W is biomass, D is plant breast diameter, H is plant height, a and b are model parameters, the plant salt reduction amount is characterized by multiplying biomass of each part of plants by sodium, potassium, calcium and magnesium content of each part of plants, and the sodium, potassium, calcium and magnesium content of the plants is measured by adopting a concentrated sulfuric acid-perchloric acid digestion-inductive coupling plasma spectrum generator.
The method comprises the following steps of: four tree species, acacia confusa, equisetum coconuts and Huang, grew most rapidly in biomass with seawater addition (fig. 10, 11, 12); the salt absorption of acacia martensii, casuarina equisetifolia, coconut and Huang was highest (indicating the best salt-reducing ability) with seawater addition treatment (fig. 13).
The utility model provides a device for screening island greening falls method of salt plant, the device that boils that disappears that perchloric acid used in the parameter comparison step includes base 1 and controller, the top of base 1 has a plurality of recesses 2 and test-tube rack 3, be equipped with on the base 1 and be used for supporting the first extensible member 9 of test-tube rack 3, be located the reagent filling subassembly of test-tube rack 3 top and be used for driving the drive module that reagent filling subassembly removed, drive module comprises X axle module 10, Y axle module 11 and Z axle module 12, reagent filling subassembly includes:
the movable block 13, the movable block 13 is arranged on the Z-axis module 12, a cavity 15 is arranged in the movable block 13, and a first pipe 16 and a second pipe 17 are arranged in the cavity 15;
the upper cover 14, upper cover 14 passes through the bolt and is connected with movable block 13 to the top of upper cover 14 is equipped with the cover body 18, and the inboard of cover body 18 is equipped with second extensible member 19 and driving piece 20, and the bottom of upper cover 14 is equipped with a plurality of barrels 22, and the inside of barrel 22 has piston 26, and the bottom is equipped with syringe needle 28, and driving piece 20 passes through drive assembly and is connected with piston 26. The lower extreme of syringe needle 28 extends to the inboard of second body 17, and drive assembly includes the drive gear 21 that is connected with drive piece 20 output and rotates the thread bush 23 of connecting at barrel 22 top, and the inboard of thread bush 23 closes soon is connected with threaded rod 25 to the outside cover is equipped with driven gear 24, and the lower extreme of threaded rod 25 is connected with piston 26, and threaded rod 25 is hollow structure, and the bottom of upper cover 14 is equipped with the gag lever post 27 of inlaying and establishing at threaded rod 25 inside. The bottom of the upper cover 14 is provided with a plurality of transmission components, driven gears 24 of the transmission components are located at different heights, a driving gear 21 is meshed with the driven gears 24 at different heights through up-and-down movement of a second telescopic piece 19, the driving gear 21 drives the driven gears 24 and a threaded sleeve 23 to rotate, a threaded rod 25 is enabled to push a piston 26 to move, reagent in a cylinder 22 is pushed out, and the reagent passes through a sealing plug 29 along a second tube 17 to enter a test tube. The driving member 20 adopts a micro servo motor, and before the second telescopic member 19 adjusts the position, the driving member 20 needs to drive the driving gear 21 to restore the initial position.
The inside of recess 2 is equipped with heliciform heating wire 4, and the cover is equipped with annular structure's insulating sheath 5 on the heating wire 4, and the material of insulating sheath 5 adopts any one of glass fiber, asbestos, rock wool. The spiral heating wire 4 is helpful for uniformly heating the test tube, and the heat insulation sleeve 5 ensures that the test tube is not easy to directly contact the heating wire 4, thereby playing a role in protection.
Be equipped with a plurality of spacing subassemblies that are used for centre gripping test tube on the test-tube rack 3, spacing subassembly includes stop collar 6, and the inboard of stop collar 6 is equipped with wind spring 7, and the inboard of wind spring 7 is equipped with at least three annular evenly distributed's lug 8. The test tube is inserted from the inner side of the limiting sleeve 6, the test tube is stretched out by the coil spring 7, the test tube is clamped by the coil spring 7 under the action of elastic force, and the test tube with different tube diameters can be clamped by the device.
The side of test-tube rack 3 is equipped with the fixed cover of cover locating the flexible end of first extensible member 9 and handle.
The first pipe body 16 is embedded in the bottom of the movable block 13, the lower end of the second pipe body 17 is located at the inner side of the first pipe body 16, and the upper end of the second pipe body 17 is of a funnel-shaped structure.
The top of test tube is equipped with sealing plug 29, and sealing plug 29's inside is equipped with a plurality of elasticity separation blade 30, and the outside parcel of elasticity separation blade 30 has elastic rubber. The elastic baffle plates 30 are of a fan-shaped structure, a plurality of elastic baffle plates 30 are combined into a circle to seal the sealing plug 29, dust is reduced from entering the test tube, the pollution to the digestion liquid is reduced, and the first tube body 16 is easy to bend after being inserted, so that reagent is convenient to inject.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.

Claims (1)

1. A method for screening island greening salt-reducing plants, comprising the steps of:
selecting sample sides, and building nine 15m×9m sample sides on the coastal kelp less than one kilometer from coastline, dividing into three groups, each group comprising three treatment areas of control, low-volume seawater addition and high-volume seawater addition, wherein the low-volume treatment addition seawater is 200mmyr -1 (approximately equivalent to 10% of the rainfall in the local year), the high-volume treatment adds 400mmyr of sea water -1 (approximately equivalent to 20% of the local annual rainfall), the control treatment added fresh water in the same volume as the low-volume treatment;
selecting and planting plants, wherein twelve common tree species in a tropical coastal region are planted in each sample, including acacia macrophylla, casuarina equisetifolia, mulberry, semen euryales, coco, pseudo-jasmine, ceris Mangifera, jatropha curcas, hibiscus, morinda citrifolia and erythrina ku, spraying seawater through a sprayer every half month after planting, and each sample is irrigated with fresh water in an equivalent amount to ensure that the plant growth is not limited by water;
parameter comparison, after one and a half years of planting, measuring the chest diameter increment, the tree height increment, biomass and salt absorption of the plant, wherein the biomass of the plant is estimated by adopting a different-speed growth model, and the model formula is W=a (D 2 H) b Wherein W is biomass, D is plant breast diameter, H is plant height, a and b are model parameters, the plant salt reduction amount is characterized by the absorption amount of sodium, potassium, calcium and magnesium in the plant (biomass of each part of the plant is multiplied by the sodium, potassium, calcium and magnesium content of each part), and the sodium, potassium, calcium and magnesium content of the plant is measured by adopting a concentrated sulfuric acid-perchloric acid digestion-inductive coupling plasma spectrum generator;
the utility model provides a device of cooking that disappears that perchloric acid that screening island greening salt reduction plant adopted disappears and use includes base (1) and controller, the top of base (1) has a plurality of recess (2) and test-tube rack (3), the inside of recess (2) is equipped with heliciform heating wire (4), be equipped with a plurality of spacing subassemblies that are used for centre gripping test tube on test-tube rack (3), be equipped with on base (1) and be used for supporting first telescoping member (9) of test-tube rack (3), be located reagent filling subassembly and be used for driving the drive module that reagent filling subassembly removed of test-tube rack (3), drive module comprises X axle module (10), Y axle module (11) and Z axle module (12), reagent filling subassembly includes:
the movable block (13), the movable block (13) is arranged on the Z-axis module (12), a cavity (15) is formed in the movable block (13), and a first pipe body (16) and a second pipe body (17) are arranged in the cavity (15);
the upper cover (14), the upper cover (14) is connected with the movable block (13) through a bolt, the top of the upper cover (14) is provided with a cover body (18), the inner side of the cover body (18) is provided with a second telescopic piece (19) and a driving piece (20), the bottom of the upper cover (14) is provided with a plurality of cylinders (22), the inside of each cylinder (22) is provided with a piston (26), the bottom of each cylinder is provided with a needle head (28), and the driving piece (20) is connected with the piston (26) through a transmission assembly;
the utility model discloses a test tube rack, including heating wire (4), including insulating sleeve (5) of annular structure, the material of insulating sleeve (5) adopts glass fiber, asbestos, rock wool any one, spacing subassembly includes stop collar (6), the inboard of stop collar (6) is equipped with coil spring (7), the inboard of coil spring (7) is equipped with at least three annular evenly distributed's lug (8), the side of test-tube rack (3) is equipped with fixed cover and the handle of cover locating the flexible end of first extensible member (9), first body (16) are inlayed and are established in the bottom of movable block (13), the lower extreme of second body (17) is located the inboard of first body (16), the upper end of the second pipe body (17) is of a funnel-shaped structure, the lower end of the needle head (28) extends to the inner side of the second pipe body (17), the transmission assembly comprises a driving gear (21) connected with the output end of the driving piece (20) and a thread sleeve (23) rotationally connected to the top of the barrel body (22), a threaded rod (25) is rotationally connected to the inner side of the thread sleeve (23), a driven gear (24) is sleeved on the outer side of the thread sleeve, the lower end of the threaded rod (25) is connected with a piston (26), the threaded rod (25) is of a hollow structure, a limit rod (27) embedded in the threaded rod (25) is arranged at the bottom of the upper cover (14), the top of test tube is equipped with sealing plug (29), the inside of sealing plug (29) is equipped with a plurality of elasticity separation blade (30), the outside parcel of elasticity separation blade (30) has elastic rubber.
CN202011401260.1A 2020-12-02 2020-12-02 Method and device for screening island greening salt-reducing plants Active CN112715195B (en)

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CN204469720U (en) * 2014-09-24 2015-07-15 深圳市爱康生物科技有限公司 A kind of Novel small-sized sample injector
CN105359856A (en) * 2015-11-11 2016-03-02 中国科学院遗传与发育生物学研究所 Method and device for batch identification of wheat salt tolerance
CN107349990A (en) * 2017-08-04 2017-11-17 韩祥 A kind of rack for test tube for being used to measure SOD
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CN211800976U (en) * 2020-03-12 2020-10-30 安徽尚德谱检测技术有限责任公司 Chemical reagent adds device for chemistry experiments
CN111617819A (en) * 2020-06-03 2020-09-04 安徽中青检验检测有限公司 Fixing frame for detecting test tube

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