CN112704739A - Preparation method of lutein preparation and lutein preparation - Google Patents

Preparation method of lutein preparation and lutein preparation Download PDF

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CN112704739A
CN112704739A CN202011592631.9A CN202011592631A CN112704739A CN 112704739 A CN112704739 A CN 112704739A CN 202011592631 A CN202011592631 A CN 202011592631A CN 112704739 A CN112704739 A CN 112704739A
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lutein
antioxidant
preparation
xanthophyll
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吴贵富
辜质纯
李汶潓
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Foshan Nanhai Eastern Along Pharmaceutical Co ltd
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Abstract

The invention discloses a preparation method of a lutein preparation, which comprises the steps of heating and melting marigold extract, adding strong base for saponification, and then diluting by adopting auxiliary materials to obtain a finished product of the lutein preparation. Wherein, during the melting and saponification process, an antioxidant is added, and the antioxidant is a mixture of ethoxyquinoline, butyl hydroxy anisole and tert-butyl benzenediol. Correspondingly, the invention also discloses a lutein preparation. The invention adopts specific antioxidant, reduces the oxidation consumption of lutein ester, and improves the content of lutein in the finished product of the lutein preparation and the color stability of the lutein preparation.

Description

Preparation method of lutein preparation and lutein preparation
Technical Field
The invention relates to the technical field of medicines, and particularly relates to a preparation method of a lutein preparation and the lutein preparation.
Background
Along with the improvement of living standard of people, the rapid development of animal husbandry is driven, and meanwhile, higher requirements on the quality and safety of animal products are also met, and the use of antibiotic additives on the market is forbidden; the safe, environment-friendly and green novel feed additive becomes the mainstream of the current development. The lutein is a natural plant pigment, is safe and environment-friendly, is widely applied to daily production of livestock, poultry and aquatic products, and is widely praised by consumers. However, the lutein in the plant body exists mainly in the form of lutein ester, which is not beneficial to be absorbed and utilized by animals. Therefore, the conversion of lutein ester in plant body into single lutein is an important processing technology. At present, the raw material of the lutein preparation sold on the market is mainly marigold extract, and in the process of processing the marigold extract to obtain the lutein product, the oxidation of lutein ester often occurs, so that the loss of the active ingredients of the lutein is caused.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a preparation method of a lutein preparation, which can effectively reduce the loss of lutein in the processing process of marigold extract.
The invention also aims to solve the technical problem of providing a lutein preparation.
In order to solve the technical problems, the invention provides a preparation method of a lutein preparation, which comprises the following steps:
(1) mixing 100 parts of marigold extract and 0.2-2.5 parts of antioxidant, and melting at a first preset temperature for a first preset time to obtain a molten liquid;
(2) mixing 100 parts of molten liquid with 0.2-2.5 parts of antioxidant, 5-10 parts of solvent, 10-20 parts of strong base and 10-15 parts of water, and saponifying at a second preset temperature for a second preset time to obtain saponified liquid;
(3) diluting the saponified solution by adopting auxiliary materials to obtain a finished product of the lutein preparation;
wherein the antioxidant is a mixture of ethoxyquinoline, butyl hydroxyanisole and tert-butyl benzenediol.
As an improvement of the above technical solution, in the antioxidant, ethoxyquinoline: butyl hydroxyanisole: tert-butylbenzenediol ═ (0.5-5): (0.1-0.5): (0.1-0.5).
As an improvement of the above technical solution, ethoxyquinoline: butyl hydroxyanisole: tert-butylbenzenediol ═ 5:0.5: 0.1.
as an improvement of the technical scheme, in the step (1), 100 parts of marigold extract is mixed with 0.5 part of antioxidant.
As an improvement of the technical scheme, the first preset temperature is 60-80 ℃, and the first preset time is 10-15 h.
As an improvement of the technical scheme, the first preset temperature is 70 ℃, and the first preset time is 14 h.
As an improvement of the technical scheme, the solvent is one or more of dimethyl sulfoxide, n-heptane, n-hexane and carbon tetrachloride;
the strong base is selected from sodium hydroxide or potassium hydroxide;
the second preset temperature is 60-80 ℃, and the second preset time is 4-7 h.
As an improvement of the technical scheme, in the step (2), 100 parts of molten liquid is mixed with 1.5 parts of antioxidant, 6 parts of dimethyl sulfoxide and 12 parts of water, then 14 parts of strong base is slowly added while stirring, and saponification is carried out for 5 hours at 75 ℃ to obtain saponified liquid.
As an improvement of the technical scheme, the lutein preparation is granules, the auxiliary material is a mixture of silicon dioxide and light calcium carbonate, and the silicon dioxide: light calcium carbonate is 1: 4.
Correspondingly, the invention also discloses a lutein preparation which is prepared by the preparation method of the lutein preparation.
The implementation of the invention has the following beneficial effects:
the preparation method of the lutein preparation takes marigold extract as a raw material, adopts a melting-saponification-auxiliary material mixing and diluting process, and adds a mixture of ethoxyquinoline, butyl hydroxy anisole and tert-butyl benzenediol as an antioxidant in the melting and saponification processes, thereby reducing the oxidation consumption of lutein ester and improving the content and color stability of lutein in a finished product of the lutein preparation.
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FIG. 1 is a flow chart of a process for the preparation of a xanthophyll formulation according to the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the accompanying drawings and detailed description.
Referring to fig. 1, the present invention provides a method for preparing a lutein preparation, which comprises the following steps:
s1: mixing marigold extract and antioxidant, and melting at a first preset temperature for a first preset time to obtain a molten liquid;
wherein the marigold extract is obtained by extracting marigold raw material with extraction solvent (such as petroleum ether, etc.), and contains lutein ester (content of about 150mg/g) as main ingredient. Marigold extract generally exists in the form of viscous gel, and the direct processing difficulty is large. The invention firstly heats and melts the raw materials to form liquid with fluidity, and then processes the liquid. Specifically, the heating and melting may be performed by heating processes such as water bath heating, hot air heating, and oil bath heating, but is not limited thereto. Preferably, the marigold extract is heated by water bath, and the heat supply is uniform, so that the melting of the marigold extract can be promoted more uniformly.
Specifically, the first predetermined temperature is 60 to 80 ℃, and exemplary temperatures are 61 ℃, 63 ℃, 65 ℃, 68 ℃, 70 ℃, 73 ℃, 76 ℃ or 78 ℃, but not limited thereto. The first preset time is 10-15h, and exemplary times may be 10h, 10.5h, 11h, 12h, 13h, 14h, 14.5h or 15h, but is not limited thereto. Preferably, the first preset temperature is 70 ℃ and the first preset time is 14 h.
In order to avoid the decomposition of the lutein ester during the melting under heating, an antioxidant is added. Specifically, when the marigold extract accounts for 100 parts by weight, the using amount of the antioxidant is 0.2-2.5 parts; illustratively, the antioxidant is used in an amount of 0.2 parts, 0.3 parts, 0.4 parts, 0.6 parts, 0.8 parts, 1.0 parts, 1.2 parts, 1.8 parts, or 2.3 parts, but is not limited thereto. Preferably, the antioxidant is used in an amount of 0.5-1.5 parts per 100 parts by weight of marigold extract.
The antioxidant is a mixture of ethoxyquinoline, butyl hydroxyanisole and tert-butylbenzenediol, and the mixture is used as the antioxidant, so that the oxidative decomposition of lutein ester in the heating and melting process can be effectively reduced, and the color stability of a lutein preparation product can be ensured.
Wherein, when the using amount of the antioxidant is 100 parts by weight, the using amount of the ethoxyquinoline is 15-95 parts by weight, the using amount of the butyl hydroxy anisole is 2-15 parts by weight, and the using amount of the tert-butyl benzenediol is 2-15 parts by weight. Illustratively, ethoxyquinoline is used in an amount of 16 parts, 20 parts, 25 parts, 30 parts, 40 parts, 50 parts, 55 parts, 60 parts, 70 parts, 82 parts, or 94 parts, but is not limited thereto; the amount of butyl hydroxy anisole is 3 parts, 4 parts, 6 parts, 8 parts, 10 parts or 13 parts, but not limited thereto; the amount of t-butylbenzenediol is 3 parts, 4 parts, 6 parts, 8 parts, 10 parts or 13 parts, but is not limited thereto. Preferably, when the antioxidant is used in an amount of 100 parts by weight, ethoxyquinoline is used in an amount of 89.3 parts by weight, butylhydroxyanisole is used in an amount of 8.9 parts by weight, and tert-butylbenzenediol is used in an amount of 1.8 parts by weight.
S2: mixing the molten liquid with an antioxidant, a solvent, strong base and water, and saponifying at a second preset temperature for a second preset time to obtain saponified liquid;
specifically, 100 parts by weight of the molten liquid is taken, mixed with an antioxidant, a solvent, strong base and water, saponified for 4-7 hours at 60-80 ℃ to obtain saponified liquid, and lutein esters in marigold extract can be converted into lutein crystals through saponification reaction.
Wherein, the antioxidant can effectively reduce the oxidation loss of lutein ester in the saponification process and ensure the content and the color stability of the finished product of the lutein preparation. The antioxidant may be used in an amount of 0.2 to 2.5 parts by weight, and may be exemplified by 0.3 part, 0.6 part, 0.8 part, 1 part, 1.1 part, 1.2 parts, 1.6 parts, 1.8 parts, 2 parts, 2.2 parts, but is not limited thereto. Preferably, the using amount of the antioxidant is 0.5-1.5 parts, and when the using amount exceeds 1.5 parts, the lutein ester loss rate is increased; more preferably, the antioxidant is used in an amount of 1.5 parts.
The solvent is helpful for saponification production, and can promote the coloring of the auxiliary materials in the later period, and improve the color stability of the finished product of the lutein preparation. Specifically, the solvent can be one or more of dimethyl sulfoxide, n-heptane, n-hexane and carbon tetrachloride; preferably, dimethyl sulfoxide is used. The solvent is used in an amount of 5 to 10 parts by weight, illustratively 5 parts, 5.4 parts, 5.7 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 9.5 parts, or 10 parts, but is not limited thereto. Preferably, the using amount of the solvent is 6-8 parts; more preferably 6 parts.
Wherein, the strong base can be selected from NaOH and KOH, but is not limited thereto; during the mixing process, the strong base should be added slowly and stirred while adding. Specifically, the strong base is used in an amount of 10 to 20 parts, illustratively 10 parts, 11 parts, 13 parts, 14 parts, 16 parts, 18 parts, or 20 parts, but is not limited thereto. Preferably, the strong base is used in an amount of 12 to 15 parts, more preferably 15 parts.
Wherein, the water should be purified water to ensure that other impurity ions are not introduced. Specifically, water is used in an amount of 10 to 15 parts, illustratively 10 parts, 11 parts, 12 parts, 13 parts, or 14 parts, but is not limited thereto. Preferably, the amount of water is 12 parts.
During saponification, the saponification temperature is 60-80 ℃, and the saponification time is 4-7 h; preferably, the saponification temperature is 75 ℃ and the saponification time is 5 h. After saponification, the lutein saponification rate should be monitored by detecting the lutein content before and after saponification by high performance liquid chromatography.
S3: diluting the saponified solution with adjuvants to obtain xanthophyll preparation;
specifically, the dosage form of the lutein preparation can be capsules, granules and powder, but is not limited to the above. The adjuvants can be selected according to specific dosage forms.
Preferably, in the invention, the lutein preparation is a granular preparation, the auxiliary material is a mixture of silicon dioxide and light calcium carbonate, and the ratio of silicon dioxide: light calcium carbonate is 1:4 (weight ratio).
Specifically, the saponified solution is diluted by adopting auxiliary materials to obtain a preparation with the required lutein content of 1-3 wt%, and a finished product of the lutein preparation is obtained.
Preferably, the lutein content in the saponified liquid is firstly measured, and then accounting is carried out according to the lutein content in the finished product, so as to determine the specific dosage of the auxiliary materials.
When the xanthophyll preparation is a granular preparation, the saponified solution, silicon dioxide and light calcium carbonate can be fully mixed in a stirring tank, and then the mixture is sieved by a 20-mesh sieve, so that powder granules are obtained.
The present invention will be described below with reference to specific examples.
EXAMPLE 1 screening of antioxidants
Taking 500g of marigold extract (product of commercial Chenguang Biotechnology group GmbH, Inc., lutein content 15.32%), adding antioxidant composition (specific addition amount is shown in Table 1), heating in water bath for melting, heating at 70 deg.C until marigold extract is completely dissolved, and standing for 14 hr. Wherein the antioxidant combination is selected from: one or more of Ethoxyquinoline (EQ), Butyl Hydroxy Anisole (BHA) and tert-butyl benzenediol (TBHQ) are combined, and the three antioxidants are proportioned by an orthogonal method. Respectively detecting the content of the lutein in the marigold extract before melting and the content of the lutein in the molten liquid after melting; and calculating the loss rate of the lutein. Lutein loss rate (total amount of lutein before melting-total amount of lutein in melt after melting)/total amount of lutein before melting × 100%. The method for detecting the content of the lutein is disclosed in GB/T21517-2008.
TABLE 1 antioxidant orthogonal screening protocol and results
Figure BDA0002867302530000051
Figure BDA0002867302530000061
And (4) test conclusion: as can be seen from Table 1, the extreme differences (EQ) > BHA > TBHQ indicate that the most significant antioxidants affecting the loss of lutein in marigold extract are EQ, then BHA and finally TBHQ. Of the three antioxidant combinations, the antioxidant combination regimen of test group 9 gave the lowest lutein loss and the best protective effect.
EXAMPLE 2 determination of the amount of antioxidant added during melting
2000g of marigold extract (product of commercial Chenguang Biotechnology group GmbH, Inc., with lutein content of 15.32%) was taken, antioxidant composition (specific addition amount shown in Table 2) was added, and the mixture was heated in water bath to melt, and the heating temperature was 70 deg.C until marigold extract was completely dissolved for 14 h. Wherein, the antioxidant is: a mixture of EQ, BHA, TBHQ; and EQ: BHA: TBHQ ═ 5:0.5:0.1 (by weight). Respectively detecting the content of the lutein in the marigold extract before melting and the content of the lutein in the molten liquid after melting; and calculating the loss rate of the lutein. The specific detection method and calculation method are as in example 1.
TABLE 2 test of the influence of different addition amounts of antioxidants on the loss of lutein during melting
Antioxidant addition/g The content of lutein in the melt/%) Lutein loss rate/%)
Comparative example 1 0 9.54 5.78
Test group 10 2g(0.1％) 13.15 2.17
Test group 11 10g(0.5％) 13.55 1.76
Test group 12 30g(1.5％) 13.59 1.70
Test group 13 50g(2.5％) 13.50 1.78
As can be seen from Table 2, the lutein loss rate was greatest in test group 10, the differences in lutein ester loss rates were not evident in test groups 11-13, and the protocol for test group 11 was chosen for cost considerations, i.e., 10g (0.5%) antioxidant addition.
EXAMPLE 3 determination of the amount of antioxidant added during saponification
The melt (lutein content 13.55%) prepared in test group 11 was divided into 4 portions of 300g each, and the antioxidant composition of test group 9 (see table 3 for specific amounts), 18g of dimethyl sulfoxide, 45g of potassium hydroxide and 36g of purified water were added to the melt, and the melt was saponified while stirring with slow addition of potassium hydroxide, and saponified at 75 ℃ for 5 hours to obtain a saponified solution. The lutein loss rate before and after saponification was calculated.
Wherein, the lutein loss rate is (the total amount of lutein in the melting liquid-the total amount of lutein in the saponification liquid)/the total amount of lutein in the melting liquid is multiplied by 100, and the lutein content is detected by an ultraviolet spectrophotometry. Wherein the total weight of the saponified solution after saponification is 300g + antioxidant amount + 18g of dimethyl sulfoxide + 45g of potassium hydroxide + 36g of purified water, namely 399g + antioxidant amount.
TABLE 3 test of the influence of different addition amounts of antioxidants on the lutein loss rate during saponification
Antioxidant agent Antioxidant addition/g in saponification process Xanthophyll content/% of saponified liquid Lutein loss rate/%)
Comparative example 2 0 3.10 7.86
Test group 14 1.5g(0.5％) 7.20 4.76
Test group 15 4.5g(1.5％) 10.82 2.03
Test group 16 7.5g(2.5％) 10.70 2.10
As can be seen from Table 3, the antioxidant was not added during the saponification process, and the lutein ester loss was large; test group 14 antioxidant composition was added at 1.5g (0.5%) with a lutein ester loss of 4.76%; the lutein ester loss rates of the test group 15 (added amount of 1.5%) and the test group 16 (added amount of 2.5%) were 2.03% and 2.10%, respectively, and the difference was not significant. The addition schedule for test group 15, i.e., the saponification process antioxidant composition addition was 1.5%, was chosen for cost economy.
Example 4
The embodiment provides a preparation method of lutein particles, which comprises the following steps:
(1) mixing 100 parts of marigold extract and 0.5 part of antioxidant, heating in water bath at 70 ℃ for 14h, and melting marigold extract to obtain molten liquid; wherein, the antioxidant is: a mixture of EQ, BHA, TBHQ; and EQ: BHA: TBHQ ═ 5:0.5:0.1 (by weight).
(2) Mixing 100 parts of molten liquid, 1.5 parts of antioxidant and 6 parts of dimethyl sulfoxide in a reaction tank, slowly adding a mixture of 15 parts of potassium hydroxide and 12 parts of purified water, and performing saponification reaction at 75 ℃ for 5 hours to obtain saponified liquid;
(3) and (3) detecting the content of the lutein in the saponified solution obtained in the step (2), adding a dilution auxiliary material (20% of silicon dioxide and 80% of light calcium carbonate) with a corresponding dilution ratio according to requirements, mixing for 25 minutes in a stirring tank, and sieving by using a 20-mesh sieve to obtain a finished product of lutein particles. If the lutein content in the saponified liquid is 10% and the lutein content in the lutein granule finished product is required to be 2%, the weight of the added diluting auxiliary material is 10/2 times of the weight of the saponified liquid. Namely 100 parts of saponification solution, 10/2 x 100 parts of 500 parts of diluting auxiliary materials are added.
Example 5
This example differs from example 4 in that the antioxidant is added in an amount of 0.2 parts in step 1, and the other steps are the same as in example 4.
Example 6
This example differs from example 4 in that the antioxidant in step 2 was added in an amount of 0.2 parts, and the other examples are the same as example 4.
Example 7
This example differs from example 4 in that the antioxidants in step 1 and step 2 are different, and the antioxidants in this example are combinations of test groups 5, namely EQ: BHA: TBHQ was 0.5:0.1:0.5 (by weight), and the same as in example 4.
Example 8
The difference between this example and example 4 is that the solvent and the strong base in step 2 are different, the solvent in this example is n-hexane, the strong base is sodium hydroxide, and the others are the same as example 4.
Example 9
The difference between this embodiment and embodiment 4 is that the first preset temperature and the first preset time in step 1 are different, in this embodiment, the first preset temperature is 60 ℃, and the first preset time is 15 h.
Example 10
The difference between this embodiment and embodiment 4 is that the second preset temperature and the second preset time in step 2 are different, where the second preset temperature is 80 ℃ and the second preset time is 4 h.
Note: the marigold extract used in the above examples is a commercially available product from chenguang biotechnology group ltd, and the lutein content is 15.32%, and the lutein granule prepared in examples 4-10 has a lutein content of 2% ± 0.2% as a qualified product. Other auxiliary materials are conventional commercial products.
The xanthophyll content of the saponified solutions prepared in examples 4 to 10 was measured. And calculating the lutein loss rate according to the content. The specific results are as follows:
table 4 test results of lutein loss rate in examples 4 to 10
Figure BDA0002867302530000081
Figure BDA0002867302530000091
Example 11 stability test
First, test sample
Prepared according to the method of examples 4-10, the saponified solution of step 2 and the final finished lutein granule were extracted and tested for stability.
Second, test protocol
Taking a proper amount of the saponified solution prepared in the step 2 by the method in the embodiment 4-10 and a final lutein granule finished product, and sequentially carrying out influence factor tests, accelerated tests and long-term tests according to appendix 302 of the pharmacopoeia of the people's republic of China (2015 edition) -veterinary drug stability test guiding principle and technical guiding principle of veterinary chemical drug stability research. The specific test scheme is as follows:
(1) influencing factor test protocol: taking 50g of finished lutein granules prepared in the step 2 in the methods of examples 4-10, packaging the finished lutein granules according to the market, respectively placing the finished lutein granules in three conditions of high temperature (60 ℃) and strong light (4500+500Lx) and high humidity (90% humidity) to carry out high temperature, strong light and high humidity influence factor tests, placing the lutein granules for 10 days, respectively sampling the lutein granules in 0 day, 5 days and 10 days, carrying out lutein content detection and color investigation, and taking the lutein granules as indexes to investigate the stability of the test samples.
(2) Accelerated test protocol: 50g of finished lutein granules prepared in step 2 by the method of example 4-10 are packaged in a commercial package and placed under the conditions of 40 +/-2 ℃ and 75% +/-5% relative humidity for 6 months. Samples were taken at the end of 0 month, 1 month, 2 months, 3 months and 6 months during the test period, and the stability of the test samples was examined by measuring the content of lutein and examining the color.
(3) Long-term test protocol: 50g of finished lutein particles prepared in step 2 in the methods of examples 4 to 10 are packaged in a commercially available package and placed at 25 ℃. + -. 2 ℃ and a relative humidity of 60%. + -. 10% for 12 months. Sampling at 0 month, 1 month, 3 months, 6 months, 9 months and 12 months respectively, and carrying out lutein content detection and color inspection to inspect the stability of the test sample by taking the lutein content detection and the color inspection as indexes.
Third, test results
TABLE 5 influence factor test results
Figure BDA0002867302530000101
Figure BDA0002867302530000111
And (4) test conclusion: as can be seen from Table 5, the finished lutein granules prepared by the technical schemes of examples 4-10 have definite properties, and the lutein content can be kept within the qualified range in 10 days under the conditions of high temperature, high humidity and strong light, which indicates that the technical scheme is feasible.
TABLE 6 accelerated test results
Figure BDA0002867302530000121
Figure BDA0002867302530000131
And (4) test conclusion: as can be seen from Table 6, the final product of lutein granule prepared by the technical schemes of examples 4-10 has stable property, and the lutein content and character can be kept within the qualified range for 6 months under the accelerated condition, which indicates that the technical scheme is feasible.
TABLE 7 Long-term stability test results
Figure BDA0002867302530000132
Figure BDA0002867302530000141
And (4) test conclusion: as can be seen from Table 7, the final lutein granules prepared by the technical schemes of examples 4-10 have stable properties, and the lutein content and character can be kept within the qualified range within 12 months under the long-term test condition, which indicates that the technical scheme is feasible.
While the foregoing is directed to the preferred embodiment of the present invention, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention.

Claims (10)

1. A method for preparing a xanthophyll formulation, comprising:
(1) mixing 100 parts of marigold extract and 0.2-2.5 parts of antioxidant, and melting at a first preset temperature for a first preset time to obtain a molten liquid;
(2) mixing 100 parts of molten liquid with 0.2-2.5 parts of antioxidant, 5-10 parts of solvent, 10-20 parts of strong base and 10-15 parts of water, and saponifying at a second preset temperature for a second preset time to obtain saponified liquid;
(3) diluting the saponified solution by adopting auxiliary materials to obtain a finished product of the lutein preparation;
wherein the antioxidant is a mixture of ethoxyquinoline, butyl hydroxyanisole and tert-butyl benzenediol.
2. The method of preparing the xanthophyll formulation of claim 1, wherein the antioxidant is selected from the group consisting of ethoxyquinoline: butyl hydroxyanisole: tert-butylbenzenediol ═ (0.5-5): (0.1-0.5): (0.1-0.5).
3. The process for the preparation of a xanthophyll formulation according to claim 1 or 2, characterized in that the ratio ethoxyquinoline: butyl hydroxyanisole: tert-butylbenzenediol ═ 5:0.5: 0.1.
4. the process for preparing a xanthophyll formulation according to claim 1 or 2, wherein in step (1), 100 parts of marigold extract is mixed with 0.5 part of antioxidant.
5. The method for preparing a xanthophyll formulation according to claim 1 or 2, wherein the first predetermined temperature is 60-80 ℃ and the first predetermined time is 10-15 h.
6. The method of preparing the xanthophyll formulation of claim 5, wherein the first predetermined temperature is 70 ℃ and the first predetermined time is 14 hours.
7. The method for preparing the xanthophyll formulation according to claim 1, wherein the solvent is one or more selected from the group consisting of dimethylsulfoxide, n-heptane, n-hexane, and carbon tetrachloride;
the strong base is selected from sodium hydroxide or potassium hydroxide;
the second preset temperature is 60-80 ℃, and the second preset time is 4-7 h.
8. The process for preparing a xanthophyll formulation according to claim 1, wherein in step (2), 100 parts of the melt is mixed with 1.5 parts of antioxidant, 6 parts of dimethyl sulfoxide and 12 parts of water, and then 14 parts of strong base is slowly added with stirring and saponified for 5 hours at 75 ℃ to obtain a saponified solution.
9. The method of preparing the xanthophyll formulation of claim 1, wherein the xanthophyll formulation is a granule, the excipient is a mixture of silica and precipitated calcium carbonate, the ratio of silica: light calcium carbonate is 1: 4.
10. A xanthophyll preparation produced by the method for producing a xanthophyll preparation according to any one of claims 1 to 9.
CN202011592631.9A 2020-12-29 2020-12-29 Preparation method of lutein preparation and lutein preparation Pending CN112704739A (en)

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