CN112694175A - Flora for degrading beta-cypermethrin under alkaline condition and enrichment method thereof - Google Patents
Flora for degrading beta-cypermethrin under alkaline condition and enrichment method thereof Download PDFInfo
- Publication number
- CN112694175A CN112694175A CN202011408132.XA CN202011408132A CN112694175A CN 112694175 A CN112694175 A CN 112694175A CN 202011408132 A CN202011408132 A CN 202011408132A CN 112694175 A CN112694175 A CN 112694175A
- Authority
- CN
- China
- Prior art keywords
- cypermethrin
- beta
- flora
- culture
- degrading
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/306—Pesticides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a flora for degrading beta-cypermethrin under an alkaline condition, which comprises the steps of collecting soil samples with different depths polluted by beta-cypermethrin components at multiple points; culturing and transferring: adding the soil sample into a minimum salt culture medium containing the beta-cypermethrin, and carrying out culture transfer at 37 ℃, wherein the bacterial liquid after the transfer culture is the bacterial colony of the beta-cypermethrin obtained by enrichment screening; and (3) diluting and separating the flora by adopting a minimum salt, LB and beef extract peptone solid culture medium with the pH of 8.0, repeatedly purifying for multiple times, and preserving the strain. The invention improves the microbial diversity in the flora, promotes the synergistic degradation of the beta-cypermethrin among multiple strains, simultaneously improves the robustness of the flora, and is more suitable for environment remediation for treating the severe change of external conditions and pollutant concentration.
Description
Technical Field
The invention relates to the field of biological floras, in particular to a floras for degrading beta-cypermethrin under an alkaline condition and an enrichment method thereof.
Background
The high-efficiency cypermethrin is a pyrethroid insecticide, because the high-efficiency cypermethrin has the characteristics of wide insecticidal spectrum, high drug effect, high safety on mammals and the like, the use amount of the high-efficiency cypermethrin in the aspects of sanitation, agriculture and the like is rapidly expanded, but along with the perennial large-scale application of the high-efficiency cypermethrin, the residue of the high-efficiency cypermethrin in the environments such as soil and the like is continuously accumulated, the ecological environment is polluted, and the quality of agricultural products and the safety of human beings are influenced. The elimination of the residue of the beta-cypermethrin is also a research hotspot since the beta-cypermethrin has three dangers to the central nervous system, the endocrine system, the reproductive system and the like of mammals, but almost no people are involved in the removal of the residue of the beta-cypermethrin under the saline-alkali condition.
Compared with physical and chemical treatment methods, the biodegradation method is considered to be an effective method with low cost and no secondary pollution, and students also consider that the method has the advantages of simple and convenient operation and good ecological environment recovery, so the method is worth popularizing and applying. At present, strains capable of degrading beta-cypermethrin, which are separated at home and abroad, belong to more than ten genera including pseudomonas, achromobacter, rhodococcus and the like, but some unsolved problems still exist, so that the related technology is difficult to be widely applied. For example, a single strain often has the problems of incomplete degradation, slow degradation, high requirement on degradation conditions, easy elimination of wild strains when entering the natural environment and the like, and importantly, the provided strain is not suitable for survival under alkaline conditions at all, so that the removal of the beta-cypermethrin residue in alkaline soil and other environments is urgently needed to be solved. Therefore, there is a need for a bacterial population for degrading beta-cypermethrin under alkaline conditions and an application thereof, which can effectively solve the above problems.
Disclosure of Invention
The invention aims to provide a bacterial flora for degrading beta-cypermethrin under an alkaline condition and application thereof.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
the invention comprises the following steps:
1) sampling: collecting soil samples of different depths polluted by the high-efficiency cypermethrin components at multiple points;
2) culturing and transferring: adding the soil sample into a minimum salt culture medium containing the beta-cypermethrin, and carrying out culture transfer at 37 ℃, wherein the bacterial liquid after the transfer culture is the bacterial colony of the beta-cypermethrin obtained by enrichment screening;
3) and (3) diluting and separating the flora by adopting a minimum salt, LB and beef extract peptone solid culture medium with the pH of 8.0, repeatedly purifying for multiple times, and preserving the strain.
Further, the time point of the transfer culture is a time point for monitoring and detecting the change of biomass in the bacterial liquid and the change of the concentration of the beta-cypermethrin in the bacterial liquid in real time and recording a growth curve, and the time point is used as a transfer culture point when the growth curve tends to be flat and before the curve is reduced.
Further, the minimum salt medium ratio comprises: NaCl 0.3-1.1 g/L, NH4NO3 0.5～1.0g/L，(NH4)2SO4 0.2～0.7g/L，K2HPO4 1.0～1.8g/L，KH2PO4 0.4～0.6g/L，MgSO4·7H20.1-0.25 g/L of O, 7.5-8.2 of pHs, 20-250 mg/L of beta-cypermethrin and the balance of distilled water.
Further, the ratio of the minimum salt culture medium of the culture solution is preferably NH4NO3 1.0g/L，MgSO4·7H2O 0.2g/L，K2HPO4 1.5g/L，KH2PO4 0.5g/L，NaCl 0.5g/L，(NH4)2SO40.5g/L of beta-cypermethrin, 100mg/L of beta-cypermethrin, adjusting the pH value to 8.0 by using NaOH, and supplementing 1000mL of distilled water.
Further, the culture conditions of the flora in the step 2 comprise that the flora is inoculated according to the volume ratio of 1-5%, and then cultured under the conditions of 37 ℃ and 120rpm and keeping out of light.
A flora for degrading beta-cypermethrin under alkaline conditions comprises Proteobacteria, Firmicutes, bacteroides and Actinobacteria at a phylum level, and the abundance ratio is 70-90: 3-25: 2-25: 50-70: 0.0001 to 3. At the genus level, Achromobacter, Hyphomicrobium, Pseudomonas, Agathobacter, Bacteroides, Rhodococcus, Shinella, Sphingopyxis, Faecalibacterium, Bordetella, Chelatococcus, Bosea, Roseburia, Sutterlla, unidentified _ Lachnospiraceae, Paramesurizobium, Dokdonella, Pseudonanobacter, Pusilmonilia, abundance ratios between 10 and 0.0000001 are included.
Further, the flora also comprises Rhodococcus _ rhodochrous, Dokdonella _ koreensis, Bacteroides _ coprocolla, Bacteroides _ vulgatus, Pseudonanobacter _ defluvivii, Bacteroides _ plexius, Paracoccus _ alcaliphilus, Bordetella _ petrii and Hyphomicium _ sp _ CS1GBMeth3, wherein the abundance ratio of the strains is 15-0.0000001.
Compared with the prior art, the invention has the following beneficial effects:
1. according to the method for degrading the high-efficiency cypermethrin flora under the enrichment culture alkaline condition, the proportion of each component of the minimum salt culture medium is optimized, the optimum pH range, the culture condition optimization and the like, and the discovery that when the optimized minimum salt proportion and concentration are adopted and the pH is 8.0, the degradation flora capable of taking the high-efficiency cypermethrin as the only C source can be enriched and cultured in soil samples of the cotton field in continuous cropping in Xinjiang under the condition of proper culture, the operation is simple, scientific and feasible, the requirement on equipment is low, and the degradation flora capable of enriching and culturing the high-efficiency cypermethrin can be enriched and cultured in a short time.
2. The invention adopts a method of adding a small amount of simple C source, even if the beta-cypermethrin which is insoluble in a minimum salt culture medium forms emulsion, the emulsion is promoted to contact with microbial cells so as to accelerate degradation, and energy substances are provided for some strains which can grow and metabolize only by the simple C source in the early stage. Finally, the microbial diversity in the flora is improved, the synergistic degradation effect of the beta-cypermethrin among multiple strains is promoted, the robustness of the flora is improved, and the method is more suitable for environment remediation for treating external conditions and severe change of pollutant concentration.
3. The functional flora obtained by screening by the method can continuously maintain the synergistic effect among various strains, the composition of the flora is stable, the composition has certain degradation capability on the pyrethroid pesticides, and the degradation capability is lasting and stable after the pyrethroid pesticides enter the nature.
Drawings
FIG. 1 is a schematic flow chart of the present invention
Detailed Description
The present invention is further illustrated by the following examples, which are intended to be in a manner including, but not limited to, the following examples.
As shown in fig. 1, example 1: enrichment culture of efficient cypermethrin degrading flora under alkaline condition
1) Soil sample collection
Collecting soil samples from long-term continuous cropping cotton fields in Xinjiang, wherein the pH value of the soil samples is above 7.8. Comprises sampling 5 pieces of land with the continuous cropping age of cotton of more than 10-30 years by a 5-point method, wherein the sampling depth is 1-40cm, and each point is used for taking equal weight of soil. The soil samples were packed in sterile plastic bags and transported to the laboratory at 4 ℃ with one portion immediately used for enrichment culture and one portion stored at-20 ℃.
2) Culturing and subculturing
Uniformly mixing the soil samples collected in the step 1) in equal amount, and adding 10g of the mixture into 100mL of liquid minimum salt culture medium containing 100mg/L of beta-cypermethrin, wherein the proportion of the minimum salt culture medium is NH4NO3 1.0g/L，MgSO4·7H2O 0.2g/L，K2HPO4 1.5g/L，KH2PO4 0.5g/L，NaCl 0.5g/L，(NH4)2SO40.5g/L, NaOH to adjust pH to 8.0, and distilled water to 1000 mL. Placing the mixture in a 250mL triangular bottle mouth, sealing the bottle mouth by using a sealing film, wherein the surface of the sealing film is provided with air holes, and placing the mixture in a 120rmp shaking table at 37 ℃ for culturing in a dark place. And measuring the biomass change by a spectrophotometry method, taking the biomass change as a time point of subculture after the biomass change is stable, wherein the concentration of the beta-cypermethrin can be gradually increased from 10-100mg/L, measuring the change condition of a growth curve within 15 days of culture, and taking the highest point as a transfer point. After transfer, inoculating according to the volume ratio of 3% for each passage, taking the uniformly mixed culture solution as an inoculated bacterial solution during transfer, and carrying out more than 5 passages under the same culture conditions to obtain the degraded flora.
3) Separation and purification:
height obtained by separating multiple times of switching by adopting dilution flat plate coating methodCypermethrin degrading flora bacterial liquid (hereinafter referred to as enriched bacterial liquid). 9mL of sterile water in 7 tubes were aligned at 10-1、10-2、10-3、10-4、10-5、10-6、10-7And numbering in sequence. Under the aseptic operation condition, 1mL of enriched bacterial liquid is sucked and placed in 9mL of aseptic water in a first tube and vibrated to obtain 10-1Bacterial liquid with concentration. The same method is used to sequentially dilute to obtain 10-7Bacterial liquid with concentration. Are respectively from 10-3、10-4、10-5、10-6、10-7The bacterial suspension (2) was applied to plates containing 100mg/L of high-efficiency cypermethrin in a minimum salt solid medium of pH 8.0, and the bacterial suspension was applied to 3 plates per concentration.
The plate inoculated with the microorganism is placed in a constant temperature and humidity incubator at 37 ℃ for culture until a colony grows out. The minimal salt solid medium was identical in composition to the minimal salt medium except that 2% agar was added. After the bacteria grow out of the flat plate, selecting the bacteria with better growth vigor for further purification.
One-loop colonies were picked aseptically using an inoculating loop, inoculated onto freshly sterilized plates, and further purified for the selected strains. Purification of the strains the dilution coating method and the streaking method were used in combination as required. The plate inoculated with the microorganism was cultured in a constant temperature and humidity incubator at 37 ℃. And identifying the purified dominant bacterial strain by adopting a PCR (polymerase chain reaction) method, wherein the PCR method comprises the steps of extracting DNA (deoxyribonucleic acid) of the dominant bacterial strain, carrying out PCR amplification on the extracted DNA by adopting a PCR amplification instrument, carrying out agarose gel electrophoresis on the DNA, and finally sequencing and analyzing the genome 16SrDNA to determine the species. The degrading bacteria obtained by screening mainly comprises Achromobacter, Hyphomicrobium, Pseudomonas, Agathobacter, Bacteroides, Rhodococcus, Shinella, Sphingopyxis, Faecalibacterium, Bordetella, Chelatococcus, Bosea, Roseburia, Sutterlla, unidentified _ Lachnospiraceae, Parasorhizobium, Dokdonella, Pseudonanobacterium, Pusilllias and a few genera which cannot be classified yet.
And (3) preservation of strains: mixing 250 μ L of glycerol and 750 μ L of bacterial liquid, and preserving at-30 deg.C.
The above-mentioned embodiment is only one of the preferred embodiments of the present invention, and should not be used to limit the scope of the present invention, but all the insubstantial modifications or changes made within the spirit and scope of the main design of the present invention, which still solve the technical problems consistent with the present invention, should be included in the scope of the present invention.
Claims (7)
1. A method for enriching, culturing and screening a bacterial colony for degrading beta-cypermethrin is characterized by comprising the following steps:
1) sampling: collecting soil samples of different depths polluted by the high-efficiency cypermethrin components at multiple points;
2) culturing and transferring: adding the soil sample into a minimum salt culture medium containing the beta-cypermethrin, and carrying out culture transfer at 37 ℃ and under the condition of pH 8.0, wherein the bacterial liquid after the transfer culture is the bacterial colony of the beta-cypermethrin obtained by enrichment screening;
3) and (3) diluting and separating the flora by adopting a minimum salt, LB and beef extract peptone solid culture medium with the pH of 8.0, repeatedly purifying for multiple times, and preserving the strain.
2. The method for enriching, culturing, screening and degrading high-efficiency cypermethrin floras according to claim 1, wherein the time point of the transfer culture is to monitor and detect the change of biomass in bacterial liquid and the change of the concentration of the high-efficiency cypermethrin in the bacterial liquid in real time and record a growth curve, and the time point is used as the transfer culture point when the growth curve tends to be flat and before the curve descends.
3. The method for screening and degrading high-efficiency cypermethrin colonies according to claim 1, wherein the minimum salt medium ratio comprises: NaCl 0.3-1.1 g/L, NH4NO3 0.5～1.0g/L，(NH4)2SO4 0.2～0.7g/L，K2HPO4 1.0～1.8g/L，KH2PO4 0.4～0.6g/L，MgSO4·7H2O 0.1～0.25g/L，pH7.5-8.2，20-250 mg/L of beta-cypermethrin, and the balance of distilled water.
4. The method for screening and degrading high-efficiency cypermethrin colonies by enrichment culture according to claim 3, wherein the ratio of the minimum salt culture medium of the culture solution is preferably NH4NO3 1.0g/L，MgSO4·7H2O 0.2g/L，K2HPO41.5g/L，KH2PO4 0.5g/L，NaCl 0.5g/L，(NH4)2SO40.5g/L of beta-cypermethrin, 100mg/L of beta-cypermethrin, adjusting the pH value to 8.0 by using NaOH, and supplementing 1000mL of distilled water.
5. The method for enriching, culturing, screening and degrading high-efficiency cypermethrin colonies according to claim 1, wherein the culture conditions of the colonies in the step 2 comprise culturing at 37 ℃ and 120rpm in the dark after inoculation according to the volume ratio of 1-5%.
6. The bacterial flora for degrading the beta-cypermethrin under the alkaline condition is characterized by comprising Proteobacteria, Firmicutes, bacteroides and actinobacillia at a phylum level, wherein the abundance ratio is 70-90: 3-25: 2-25: 50-70: 0.0001 to 3. At the genus level, Achromobacter, Hyphomicrobium, Pseudomonas, Agathobacter, Bacteroides, Rhodococcus, Shinella, Sphingopyxis, Faecalibacterium, Bordetella, Chelatococcus, Bosea, Roseburia, Sutterlla, unidentified _ Lachnospiraceae, Paramesurizobium, Dokdonella, Pseudonanobacter, Pusilmonilia, abundance ratios between 10 and 0.0000001 are included.
7. The bacterial population for degrading beta-cypermethrin under alkaline conditions, according to claim 6, wherein the bacterial population further comprises Rhodococcus rhodochrous, Dokdonella koreensis, Bacteroides coprococola, Bacteroides vulgatus, Pseudonanobacter defluvium, Bacteroides plebeius, Paracoccus alcaliphilius, Bordetella petrii and Hyphomicrobium sp CS1GBMeth3, with a strain abundance ratio of 15 to 0.0000001.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011408132.XA CN112694175A (en) | 2020-12-04 | 2020-12-04 | Flora for degrading beta-cypermethrin under alkaline condition and enrichment method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011408132.XA CN112694175A (en) | 2020-12-04 | 2020-12-04 | Flora for degrading beta-cypermethrin under alkaline condition and enrichment method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112694175A true CN112694175A (en) | 2021-04-23 |
Family
ID=75506179
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011408132.XA Pending CN112694175A (en) | 2020-12-04 | 2020-12-04 | Flora for degrading beta-cypermethrin under alkaline condition and enrichment method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112694175A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113388553A (en) * | 2021-07-28 | 2021-09-14 | 重庆理工大学 | Ammonia nitrogen resistant composite microbial inoculum and application and preparation method thereof |
| CN116555066A (en) * | 2022-09-06 | 2023-08-08 | 中国科学院南京土壤研究所 | Efficient PBAT agricultural film degrading bacterium and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102757897A (en) * | 2012-07-26 | 2012-10-31 | 贵阳学院 | Screening method of fenpropathrin pesticide degradation bacterium |
| CN102899269A (en) * | 2012-09-18 | 2013-01-30 | 宁波大学 | Cypermethrin degrading bacteria in marine environment, and separation, purification and application thereof |
| US20150321933A1 (en) * | 2014-05-08 | 2015-11-12 | The Trustees Of Princeton University | Methods and compositions for nitrogen removal using feammox microorganisms |
| US20160115063A1 (en) * | 2013-06-12 | 2016-04-28 | Renescience A/S | Methods of processing municipal solid waste (msw) using microbial hydrolysis and fermentation |
| CN110628673A (en) * | 2019-09-27 | 2019-12-31 | 上海交通大学 | Screening method and application of phenol degrading strain |
| FR3091865A1 (en) * | 2019-01-21 | 2020-07-24 | Veolia Water Solutions & Technologies Support | Biological process for the desalination of water to be treated |
-
2020
- 2020-12-04 CN CN202011408132.XA patent/CN112694175A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102757897A (en) * | 2012-07-26 | 2012-10-31 | 贵阳学院 | Screening method of fenpropathrin pesticide degradation bacterium |
| CN102899269A (en) * | 2012-09-18 | 2013-01-30 | 宁波大学 | Cypermethrin degrading bacteria in marine environment, and separation, purification and application thereof |
| US20160115063A1 (en) * | 2013-06-12 | 2016-04-28 | Renescience A/S | Methods of processing municipal solid waste (msw) using microbial hydrolysis and fermentation |
| US20150321933A1 (en) * | 2014-05-08 | 2015-11-12 | The Trustees Of Princeton University | Methods and compositions for nitrogen removal using feammox microorganisms |
| FR3091865A1 (en) * | 2019-01-21 | 2020-07-24 | Veolia Water Solutions & Technologies Support | Biological process for the desalination of water to be treated |
| CN110628673A (en) * | 2019-09-27 | 2019-12-31 | 上海交通大学 | Screening method and application of phenol degrading strain |
Non-Patent Citations (1)
| Title |
|---|
| 排孜丽亚•帕尔哈提等: "吡虫啉降解菌群结构解析及菌株资源挖掘", 《微生物学杂志》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113388553A (en) * | 2021-07-28 | 2021-09-14 | 重庆理工大学 | Ammonia nitrogen resistant composite microbial inoculum and application and preparation method thereof |
| CN113388553B (en) * | 2021-07-28 | 2023-05-16 | 重庆理工大学 | Ammonia nitrogen-resistant composite microbial inoculant, application and preparation method thereof |
| CN116555066A (en) * | 2022-09-06 | 2023-08-08 | 中国科学院南京土壤研究所 | Efficient PBAT agricultural film degrading bacterium and application thereof |
| CN116555066B (en) * | 2022-09-06 | 2024-02-20 | 中国科学院南京土壤研究所 | Efficient PBAT agricultural film degrading bacterium and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109055282B (en) | Novel Klebsiella pneumoniae strain and separation method and application thereof | |
| CN110283741B (en) | Rose color-changing bacterium with function of efficiently degrading polycyclic aromatic hydrocarbon and application thereof | |
| CN112694175A (en) | Flora for degrading beta-cypermethrin under alkaline condition and enrichment method thereof | |
| CN108823122B (en) | 2,4-dichlorophenol degrading bacterium and degrading method | |
| CN109337832B (en) | High-ammonia-nitrogen-resistant heterotrophic nitrification-aerobic denitrification ochrobactrum and application thereof | |
| CN109929785B (en) | Bacterium capable of degrading 2, 6-dimethylphenol and microbial inoculum produced by same | |
| CN103031256B (en) | Polycyclic aromatic hydrocarbon degrading bacterium suitable for electric field condition and application thereof | |
| CN108277175A (en) | 2,4 dinitrotoluene (DNT) sulfonate efficient degrading bacterial strain Microbacterium sp.X3 and its application | |
| CN108048365A (en) | One plant of 2,4-DNT sulfonate degradation bacteria strains and its application | |
| CN112251362B (en) | Aspergillus for degrading triphenyl phosphate and tricresyl phosphate and screening method and application thereof | |
| CN114180728A (en) | Application of citrobacter freundii in efficient degradation of DMAc | |
| US20230055742A1 (en) | Strain of Beijerinckia Fluminensis and Application Thereof in Arsenic Oxidation | |
| CN108034613B (en) | Strain Sphingomonas sp.X4 capable of efficiently degrading 2, 4-dinitrotosylate and application thereof | |
| CN102864086B (en) | Strain capable of degrading polycyclic aromatic hydrocarbons (PAHs) and application thereof in soil remediation | |
| CN115491325A (en) | Metal-tolerant copper greedy bacterium and application method thereof | |
| CN112646742B (en) | Activated sludge culture agent and application thereof | |
| CN115141770A (en) | Bacillus tequilensis H1 capable of degrading COD in livestock wastewater and application thereof | |
| Hassanshahian | Isolation and characterization of diazinon degrading bacteria from contaminated agriculture soils | |
| CN113583918A (en) | River sediment degrading strain and application thereof | |
| CN116688418B (en) | Method for degrading 3, 6-dibromocarbazole by adopting streptomyces | |
| CN110669716A (en) | Method for separating high-concentration phenol and heavy metal resistant and low-temperature resistant rhodococcus | |
| CN114410551B (en) | Pesticide intermediate degrading strain, culture method, microbial inoculum and application thereof | |
| CN114134072B (en) | Novel efficient denitrification Rheinheimia and application thereof | |
| CN113862177B (en) | Lysogen-aminoglutaric acid bacillus for synchronously degrading mixed phenol and application thereof | |
| CN115992058B (en) | Raoultella ornithinolytica PN-1, and culture method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210423 |
|
| RJ01 | Rejection of invention patent application after publication |