CN112691794A

CN112691794A - Cell centrifuge for gene detection

Info

Publication number: CN112691794A
Application number: CN202110311023.4A
Authority: CN
Inventors: 邓卓豪
Original assignee: Cytate Biomedical(guangzhou) Technologies Inc
Current assignee: Cytate Biomedical(guangzhou) Technologies Inc
Priority date: 2021-03-24
Filing date: 2021-03-24
Publication date: 2021-04-23
Anticipated expiration: 2041-03-24
Also published as: CN112691794B

Abstract

The invention relates to the technical field of biology, in particular to a cell centrifuge for gene detection, which comprises a motor, wherein an output shaft is arranged at one side of the motor, an up-and-down reciprocating device is arranged at the other end of the output shaft, a first bearing seat is fixed in the middle of the upper end of the up-and-down reciprocating device, an outer thread rod which is vertically upwards is arranged in the middle of the first bearing seat, a hollow inner thread round block is in threaded connection with the middle of the outer thread rod, an L-shaped supporting rod is fixed at one side of the hollow inner thread round block, and an up-and-down reciprocating device, an outer thread rod, a hollow inner thread round block, a direction-changing buffer device, a rotating disc, a placing device and an anti-flutter device are arranged to enable a placing tube provided with cells to perform intermittent forward rotation and reverse rotation and perform up-and-down reciprocating motion simultaneously, so as, the intervention of the turning buffer device avoids the crushing of the placing pipe caused by the overlarge instantaneous reverse acceleration.

Description

Cell centrifuge for gene detection

Technical Field

The invention relates to the technical field of biology, in particular to a cell centrifuge for gene detection.

Background

Through retrieval, the prior art discloses a cell centrifuge convenient for taking and placing test tubes, which comprises an outer box body and an inner box body, wherein the inner box body is arranged in the outer box body through a lifting device, a support frame is fixed in the inner box body, and a plurality of placing tubes are arranged on the support frame at equal intervals along the circumferential direction; the placing pipe comprises a fixed pipe body positioned on the closed side and a movable pipe body positioned on the opening side; the device is characterized in that a motor for driving the inner box body to rotate is mounted at the bottom of the inner cavity of the outer box body, a lifting design is adopted, a test tube is convenient to take, the test tube is fixed by using a placing tube, shaking in the lifting process can be effectively avoided, the device is safer to use, the placing tube consisting of the fixed tube body and the movable tube body can adjust the depth, the exposed size of the test tube is increased, operators can conveniently take the test tube, meanwhile, by arranging the connecting rod and the traction rope, the device can automatically complete the shrinkage of the movable tube body into the fixed tube body in the process that the inner box body extends out of the outer box body, the working efficiency is improved, but the device only realizes the unidirectional rotation of the placing tube with cells, cannot play a good centrifugal effect, the time required by centrifugation is longer, and the improvement of the efficiency of the gene detection.

Disclosure of Invention

The present invention is directed to a cell centrifuge for gene detection, which solves the above problems of the prior art.

In order to achieve the purpose, the invention provides the following technical scheme:

the utility model provides a cell centrifuge for gene testing, includes the motor, and the output shaft is installed to motor one side, and the up-and-down reciprocating motion device is installed to the output shaft other end, and up-and-down reciprocating motion device upper end middle part is fixed with first bearing frame, and first bearing frame mid-mounting has vertical ascending external screw thread pole, and external screw thread pole middle part threaded connection has cavity internal thread circle piece, and cavity internal thread circle piece one side is fixed with L type bracing piece, first body of rod is installed to external screw thread pole upper end, and diversion buffer installs in the first body of rod upper end, and the turning disc is installed in the diversion buffer outside, and the placing fluting of a plurality of evenly distributed is seted up in the turning disc outside, places the inside placer that all installs of fluting, be connected through preventing.

As a further scheme of the invention: the placing device comprises a third bearing for placing the two sides of the groove to be fixed, a second rod body is arranged in the middle of the third bearing, and the second rod body is connected with the second rod body through a fixed ring.

As a further scheme of the invention: and rubber round pads are arranged on the inner sides of the fixed rings.

As a further scheme of the invention: the anti-vibration device comprises a second bearing fixed on the upper portion of the first rod body, a first circular plate is fixed on the outer side of the second bearing, and the first circular plate is fixedly connected with the rotating disc through a hollow circular ring.

As a further scheme of the invention: the turning buffer device comprises a circular groove formed in the middle of the rotating disc, and the outer side of the circular groove is connected with the first rod body through a clockwork spring.

As a further scheme of the invention: the up-and-down reciprocating motion device comprises a rectangular frame, a plurality of uniformly distributed passive teeth are mounted on two sides of the interior of the rectangular frame, and the passive teeth on two sides are intermittently meshed with non-full-tooth gears.

As a further scheme of the invention: the motor is connected with a control switch through a lead.

As a further scheme of the invention: the supporting legs are installed at four corners of the lower end of the motor, and the supporting legs are connected with the L-shaped supporting rods through the bottom plate.

As a further scheme of the invention: and four corners of the lower end of the bottom plate are provided with suckers.

As a still further scheme of the invention: and the middle part of the upper end of the rotating disc is covered with a sound insulation plate.

Compared with the prior art, the invention has the beneficial effects that: through up-and-down reciprocating motion device, primary shaft bearing, the external screw thread pole, cavity internal thread circle piece, diversion buffer, the rotating disc, placer and prevent that setting up of quivering device makes the pipe of placing that is equipped with the cell carry out intermittent forward rotation and antiport, forward rotation still carries out up-and-down reciprocating motion with antiport simultaneously, and then realizes the quick centrifugation of cell, this device is in forward rotation and antiport's the twinkling of an eye, diversion buffer intervenes, avoided because of the too big pipe breakage of placing that leads to of instantaneous reverse acceleration.

Drawings

FIG. 1 is a schematic view of a cell centrifuge for gene detection.

FIG. 2 is a schematic view showing the structure of a circular groove in a cytospin for gene detection.

FIG. 3 is a schematic view showing a non-full-tooth gear in a cytospin for gene detection.

FIG. 4 is a schematic view showing the structure of a rotating disk in a cytospin for gene detection.

FIG. 5 is a schematic view showing a structure of a spiral spring in a cytospin for gene detection.

FIG. 6 is a schematic view showing the structure of a third bearing in a cytospin for gene detection.

FIG. 7 is a schematic view showing the structure of a suction cup in a cytospin for gene detection.

FIG. 8 is a schematic structural view of a first circular plate in a cytospin for gene detection.

In the figure: 1-sucker, 2-bottom plate, 3-L-shaped support rod, 4-support leg, 5-motor, 6-output shaft, 7-non-full-tooth gear, 8-rectangular frame, 9-first bearing seat, 10-hollow internal thread round block, 11-external thread rod, 12-first rod body, 13-second bearing, 14-first circular plate, 15-hollow circular ring, 16-rotating circular disc, 17-placing slot, 18-third bearing, 19-second rod body, 20-rubber round pad, 21-fixed circular ring, 22-clockwork spring, 23-round slot and 24-driven tooth.

Detailed Description

It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing and simplifying the description, but do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore are not to be construed as limiting the invention, and further, the terms "first", "second", etc., are used only for descriptive purposes and are not intended to indicate or imply relative importance or to implicitly indicate the number of technical features indicated, whereby the features defined as "first", "second", etc., may explicitly or implicitly include one or more of such features, in the description of the present invention, "a plurality" means two or more unless otherwise specified.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; the two elements may be directly connected or indirectly connected through an intermediate medium, and the two elements may be communicated with each other, and the specific meaning of the above terms in the present invention will be understood by those skilled in the art through specific situations.

The present invention will be described in detail below with reference to the embodiments with reference to the attached drawings.

Example 1

Referring to fig. 1 to 8, in an embodiment of the present invention, a cytocentrifuge for gene detection includes a motor 5, an output shaft 6 is installed at one side of the motor 5, an up-and-down reciprocating device is installed at the other end of the output shaft 6, a first bearing seat 9 is fixed at the middle of the upper end of the up-and-down reciprocating device, an upward vertical external screw rod 11 is installed at the middle of the first bearing seat 9, a hollow internal screw block 10 is connected to the middle of the external screw rod 11 through a screw thread, an L-shaped support rod 3 is fixed at one side of the hollow internal screw block, first body of rod 12 is installed to 11 upper ends of external screw thread pole, and diversion buffer is installed to 12 upper ends of the first body of rod, and rotating disc 16 is installed in the diversion buffer outside, and rotating disc 16 sets up placing slot 17 of a plurality of evenly distributed in the outside, places the inside placer that all installs of slot 17, be connected through preventing quivering between 12 upper portions of the first body of rod and the rotating disc 16.

Further, the placing device comprises a third bearing 18 for placing the two sides of the groove 17 to be fixed, a second rod body 19 is arranged in the middle of the third bearing 18, the second rod body 19 is connected through a fixed ring 21, and a rubber round pad 20 is arranged on the inner side of the fixed ring 21.

Further, the anti-vibration device comprises a second bearing 13 fixed on the upper portion of the first rod body 12, a first circular plate 14 is fixed on the outer side of the second bearing 13, and the first circular plate 14 is fixedly connected with the rotating disc 16 through a hollow circular ring 15 and used for ensuring that the rotating disc 16 always rotates by taking the first rod body 12 as the center and preventing vibration generated by eccentricity.

Further, the turning buffer device comprises a circular groove 23 formed in the middle of the rotating disc 16, the outer side of the circular groove 23 is connected with the first rod body 12 through a clockwork spring 22, and the clockwork spring 22 buffers impact force caused by reverse rotation of the first rod body 12.

Further, the up-and-down reciprocating device comprises a rectangular frame 8, a plurality of driven teeth 24 which are uniformly distributed are arranged on two sides in the rectangular frame 8, and the driven teeth 24 on two sides are intermittently meshed with the non-full-tooth gears 7.

Further, motor 5 is connected with control switch through the wire for control motor 5's rotational speed, supporting leg 4 is all installed in 5 lower extreme four corners of motor, connects through bottom plate 2 between supporting leg 4 and the L type bracing piece 3, sucking disc 1 is all installed in 2 lower extreme four corners of bottom plate, is used for making things convenient for this device fixed.

Example 2

In another embodiment of the present invention, the embodiment is different from the above-mentioned embodiments in that a sound insulation plate is coated on the middle of the upper end of the rotating disk 16 for reducing the noise generated by the extension and contraction of the power spring 22.

The working principle of the invention is as follows: placing a cell containing tube to be centrifuged into a fixed ring 21, starting a motor 5, driving an output shaft 6 to rotate by the motor 5, driving a non-full-tooth gear 7 to rotate by the output shaft 6, intermittently meshing the non-full-tooth gear 7 with driven teeth 24 on the left and right sides, driving a rectangular frame 8 to reciprocate up and down by the driven teeth 24, driving an external screw rod 11 to reciprocate up and down under the action of a hollow internal screw thread round block 10 by the rectangular frame 8 through a first bearing seat 9, rotating intermittently in forward and reverse directions, further driving a first rod 12 to reciprocate up and down and intermittently rotate in forward and reverse directions, driving a clockwork spring 22 to reciprocate up and down by the first rod 12, rotating intermittently rotating in forward and reverse directions, buffering a horizontally rotating force by the clockwork spring 22 and transmitting the buffered force to a rotating disc 16, realizing the up and down reciprocating motion of the rotating disc 16 and the forward and reverse rotation at the same time, driving the cell containing tube to reciprocate up and down by the, meanwhile, the placing device provides the degree of freedom for the cell placing tube to rotate towards the outer side, so that the cell placing tube finally realizes the up-and-down reciprocating motion, the forward reciprocating rotation and the reverse reciprocating rotation and the angular rotation around the second rod body 19, and further the rapid centrifugation of the cells is realized, wherein the anti-vibration device is arranged to ensure that the rotating disc 16 always rotates around the first rod body 12, and the eccentric rotation of the rotating disc 16 under the action of the clockwork spring 22 is avoided.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims

1. A cell centrifuge for gene detection comprises a motor (5) and is characterized in that an output shaft (6) is installed on one side of the motor (5), an up-and-down reciprocating device is installed at the other end of the output shaft (6), a first bearing seat (9) is fixed in the middle of the upper end of the up-and-down reciprocating device, a vertically upward external threaded rod (11) is installed in the middle of the first bearing seat (9), a hollow internal threaded round block (10) is in threaded connection with the middle of the external threaded rod (11), an L-shaped supporting rod (3) is fixed on one side of the hollow internal threaded round block (10), a first rod body (12) is installed at the upper end of the external threaded rod (11), a turning buffer device is installed at the upper end of the first rod body (12), a rotating disc (16) is installed on the outer side of the turning buffer device, a plurality of uniformly distributed placing grooves (17) are, the upper part of the first rod body (12) is connected with the rotating disc (16) through a vibration prevention device.

2. The cytocentrifuge for gene testing according to claim 1, wherein the placing device comprises third bearings (18) fixed at both sides of the placing slot (17), the second rods (19) are mounted at the middle parts of the third bearings (18), and the second rods (19) are connected through a fixing ring (21).

3. The cytocentrifuge of claim 2, wherein rubber pads (20) are installed on the inner sides of the fixing rings (21).

4. The cytocentrifuge for gene testing according to claim 1, wherein the anti-vibration device comprises a second bearing (13) fixed on the upper part of the first rod body (12), a first circular plate (14) is fixed on the outer side of the second bearing (13), and the first circular plate (14) is fixedly connected with the rotating disc (16) through a hollow circular ring (15).

5. The cytocentrifuge for gene testing according to claim 1, wherein the direction-changing buffer device comprises a circular groove (23) formed in the middle of the rotating disk (16), and the outer side of the circular groove (23) is connected with the first rod body (12) through a clockwork spring (22).

6. The cytocentrifuge for gene testing according to claim 1, wherein said up-and-down reciprocating device comprises a rectangular frame (8), a plurality of evenly distributed passive teeth (24) are installed on both sides of the inside of the rectangular frame (8), and said passive teeth (24) on both sides are intermittently engaged with non-full-tooth gears (7).

7. The cytocentrifuge for gene assaying according to claim 1, wherein the motor (5) is connected to a control switch through a wire.

8. The cytocentrifuge for gene testing according to claim 1, wherein the support legs (4) are installed at four corners of the lower end of the motor (5), and the support legs (4) are connected with the L-shaped support rods (3) through the bottom plate (2).

9. The cytocentrifuge for gene testing according to claim 8, wherein four corners of the lower end of the bottom plate (2) are provided with suction cups (1).

10. The cytospin for gene assay according to claim 1 or 2, wherein the rotating disk (16) is covered with a sound insulation plate at the upper middle part.