CN112690453A

CN112690453A - Composition, compound oil and application thereof

Info

Publication number: CN112690453A
Application number: CN202011615952.6A
Authority: CN
Inventors: 向力平; 蔡苏惠; 江雪媛; 王卓
Original assignee: Beijing Changchunteng Economic And Trade Co ltd
Current assignee: Beijing Changchunteng Economic And Trade Co ltd
Priority date: 2020-12-30
Filing date: 2020-12-30
Publication date: 2021-04-23

Abstract

The invention provides a composition, composite oil and application thereof, wherein the composition comprises LA, ALA and CBD in a mass ratio of (7-9) to (2-3) to 1; the composite oil comprises hemp seed oil, krill oil and tetrahydrodiphenol in a mass ratio of (10-20) to (1-6) to 1. The invention effectively solves the problem that the substance type for reducing the level of the inflammatory factors disclosed in the prior art has no remarkable effect on improving the imbalance of the immune system of a human body and/or the inflammatory physique; the provided composition and composite oil have the advantage of remarkably improving the imbalance of the human immune system and/or the constitution of chronic inflammation.

Description

Composition, compound oil and application thereof

Technical Field

The invention relates to the field of nourishment, and in particular relates to a composition, compound oil and application thereof.

Background

Healthy people have an immune system with ingenious design and balanced operation. The human immune system is the best defense of the body against infectious agents such as bacteria or viruses, and is a complex network of white blood cells, immune bodies and antibodies. When we are injured, it can help wound healing and protect our body from invasion of foreign virus, and it can also prevent tumor or cancer lesion caused by gene mutation when our body cell regenerates. The immune system of the human body can properly resist the germ infection, treat wounds and kill cancer cells. When the function of the immune system is good, white blood cells with proper quantity and sound form and immune bodies cooperate to fight against invaders and kill the invaders at one time.

The proper immune response is what we have in mind health, but if for some reason the immune system is not balanced, it can be dangerous to human health. Such as hyper-reactivity of the immune system, and so-called "allergy", almost all substances can become allergens, such as dust, pollen, drugs or food, which act as antigens to stimulate the body to produce abnormal immune reactions, thereby causing allergic rhinitis, allergic asthma, urticaria (urticaria), allergic conjunctivitis, food allergy, food intolerance, etc., or the immune system reacts to its own tissue cells inside the body to produce autoimmune diseases. For example, when our body is inflamed, the immune system that is too "excited" may be mistaken for bacterial or viral invasion, and the defense is performed urgently, and the own tissues or cells are mistaken for harmful microorganisms to attack, and as a result, autoimmune diseases such as rheumatoid arthritis may be caused. On the contrary, if the immune system is low or even absent, repeated infection can occur, and immunodeficiency diseases and tumors can be caused.

Chronic inflammation is one of the manifestations of an imbalance in the immune system, a systemic inflammation that lasts for months or years, is associated with almost all health problems and diseases, and is harmful to the body. Such as: it has been found that chronic inflammation is a common factor causing many health problems, and many human diseases are associated with chronic inflammation, including metabolic syndrome, non-alcoholic fatty liver disease, diabetes, cancer, senile dementia, obesity, cardiovascular diseases, skin problems, autoimmune diseases, etc., and the course and cause of the diseases are associated with the inflammatory state of the body, even in a sense that the diseases themselves are a long-term low-level inflammatory state, and the level of inflammatory factors is an extremely important index in the treatment process of the diseases, especially in the prognosis prediction.

However, the substances for reducing the level of inflammatory factors disclosed in the prior art are not very effective in improving the imbalance of the immune system and/or the inflammatory body condition of the human body, and further research on the components is needed to provide a substance composition capable of remarkably improving the imbalance of the immune system and the chronic inflammatory body condition of the human body.

Disclosure of Invention

Therefore, the invention solves the problem that the substance type for reducing the level of the inflammatory factors disclosed in the prior art has no remarkable effect on improving the imbalance of the human immune system and/or the inflammatory physique, and provides the composition or the compound oil for obviously reducing the level of the inflammatory factors and simultaneously improving the imbalance of the human immune system and/or the chronic inflammatory physique.

A composition comprising alpha-linolenic acid (ALA), Linoleic Acid (LA) and tetrahydrodiphenol (CBD, also known as cannabidiol), wherein the mass ratio of LA, ALA and CBD is: (7-9):(2-3):1.

The invention also comprises eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and antioxidant substances, wherein the mass ratio of the EPA, the DHA, the CBD and the antioxidant substances is (43-45): 25-26): 38-39): 1;

specifically, the mass ratio of the antioxidant substances to CBD, DHA, EPA, ALA and LA is as follows:

the antioxidant substance comprises CBD, DHA, EPA, ALA, LA 1, (38-39), (25-26), (43-45), (100-105) and (330-340).

The antioxidant substances are alpha-tocopherol, gamma-tocopherol and astaxanthin, wherein the alpha-tocopherol is selected from the group consisting of alpha-tocopherol, gamma-tocopherol and astaxanthin; gamma-tocopherol: the mass ratio of the astaxanthin is 1 (2-4) to (4-5).

The compound oil comprises hemp seed oil, krill oil and tetrahydrodiphenol, wherein the mass ratio of the hemp seed oil to the krill oil to the tetrahydrodiphenol is (10-20) to (1-6) to 1.

The composition or the compound oil is used in products for improving the human immune system imbalance or/and the chronic inflammatory physique.

Can improve the imbalance of the human immune system and/or the constitution of chronic inflammation by inhibiting the chronic inflammation of the body.

Can be used for improving human immune system imbalance and/or chronic inflammatory body constitution by regulating balance of essential fatty acid and endocannabinoid system.

Through regulating the level of inflammatory factors and the overall level of endogenous cannabinoid AEA, the oxidative stress and inflammatory response of a human body are improved.

The level of the anti-inflammatory factor IL-10 is improved by reducing the levels of proinflammatory factors IL-6, TNF-alpha and C-reactive protein related cytokines, thereby improving the oxidative stress and inflammatory response of a human body.

The health-care wine can reduce the risks of cardiovascular and cerebrovascular diseases and cancers by improving the constitution of chronic inflammation, improve the symptoms of chronic inflammation, such as hyperlipidemia, hyperglycemia, hyperhomocysteinemia, cholesterol abnormality and obesity, promote the health of skin, improve the hypothyroidism and endocrine dyscrasia, relieve the symptoms of mental and nervous systems, relieve chronic pain, protect the health of brain, improve autoimmune diseases, improve metabolic syndrome and improve hyperactivity.

The autoimmune diseases include but are not limited to rheumatoid arthritis, thyroiditis and the like.

The technical scheme of the invention has the following advantages:

1. the invention provides a novel composition, which comprises alpha-linolenic acid (ALA), Linoleic Acid (LA) and tetrahydrodiol (CBD) in specific proportions, wherein the alpha-linolenic acid (ALA) is one of Omega-3 fatty acids, the Linoleic Acid (LA) is one of Omega-6 fatty acids, and through the matching of the specific proportions of the alpha-linolenic acid (ALA) and the Linoleic Acid (LA), the health requirement of a human body on the Omega-3 polyunsaturated fatty acids is met, the conversion of arachidonic acid, a proinflammatory factor generated by metabolism of the Omega-6 Linoleic Acid (LA), to endocannabinoid 2-AG is reduced, and the inflammatory response brought by the overreaction of endocannabin 2-AG is inhibited; that is, it is effective in promoting the balance of Omega-3 fatty acids and reducing the increase of inflammatory factors in the human body, which is generally caused by the excessive intake of Omega-6 fatty acids. At the same time, supplementation with exogenous cannabinoid CBD, through its inhibitory effect on the catabolic enzyme FAAH, increases the levels of the anti-inflammatory endocannabinoid AEA and balances the imbalance of the endocannabinoid system, acting synergistically with the endocannabinoid, can act as an anti-inflammatory agent to help modulate the immune response, allowing the body to self-recover through better pathways and methods; through the synergistic interaction of ALA, LA and CBD, the traditional Chinese medicine composition acts on different receptors of a human body through different metabolic pathways and different transformation modes in the human body, and achieves multiple effects of improving inflammatory physique, improving immune imbalance and improving immunity.

2. The composition also contains EPA, DHA and antioxidant substances; among them, EPA, DHA is in phospholipid form, because phospholipid has bipolar property, it can be mixed with water to make it enter cell more easily, move in liquid form and have more biological availability; simultaneously, EPA and DHA are effective in treating metabolic disorder, and can improve various metabolic disorders such as glycometabolism, lipid metabolism and the like; the intake of EPA and DHA can reduce the level of endocannabinoid 2-AG which is usually overactive in a chronic inflammatory reaction organism caused by insufficient intake of ALA, thereby synchronously regulating and improving the inflammatory immune response of a human body caused by endocannabinoid imbalance; the antioxidant substances can eliminate free radicals inside and outside human tissue cells to play an antioxidation role, and can also assist other vitamins to play an effective physiological function, so that the human inflammatory immune response caused by the imbalance of the endocannabinoid can be better and synchronously regulated and improved through the optimization and the supplement and intake of the dietary proportion.

3. The interaction between ALA, LA, CBD, DHA, EPA and antioxidant contained in the composition of the invention can also generate 'triple cell membrane synergistic effect', which is specifically as follows:

cells are the basic units of all living activities, structures and functions of human bodies and other organisms. All physiological functions and biochemical reactions in the body are carried out on the basis of the cells and the substances they synthesize and excrete. Obviously, to maintain normal vital activities, not only the cell contents cannot be lost, but also the chemical composition must remain relatively stable, which requires a specific barrier between the cell and its environment, which can maintain the cell in a steady state during metabolism, thus maintaining the vital activities of the cell.

In fact, all animal cells are wrapped by a layer of film, called cell membrane, which is located on the cell surface, is an elastic semipermeable membrane mainly composed of phospholipids, has a membrane thickness of 7-8 nm, is composed of lipids and proteins, and is one of the biological membranes. In the course of the emergence of living matter on earth and its long-term evolution from simple to complex, the appearance of biofilms is a leap, which separates the cell contents from the cell's surroundings, and which enables the cells to exist both independently of the environment and to sustain living activities through selective exchange of matter between the biofilm and the surroundings. Therefore, the cell membrane is a selective permeable membrane with special structure and function, is a barrier between cells and the environment, and is an important structural basis for maintaining the intracellular environment stability and regulating the normal vital activities of the cells. It plays an important role in various vital metabolic activities such as substance transport inside and outside cells, intercellular recognition, cellular immunity, information transfer and metabolic regulation, and thus, the normality of cell membrane components and the stability of membrane structure are the prerequisite for its physiological function.

Polyunsaturated fatty acids are important energy sources for animals, are also important components of cell membrane phospholipids, and play an important role in cell surface signal transduction. Inside the cell membrane bilayer, DHA and EPA, phospholipids and their additional fatty acids are the molecular basis of the membrane. Studies have shown that polyunsaturated fatty acids determine the fluidity and deformability of cell membranes, and that a suitable degree of fluidity and deformability is a prerequisite for cells to maintain normal physiological function. Meanwhile, the polyunsaturated fatty acid also influences the function of the cell membrane by changing the fluidity and deformability of the membrane, so that the excess or lack of the polyunsaturated fatty acid in the cell membrane phospholipid and the ratio of Omega-6 linoleic acid to Omega-3 are increased to cause the change of the fluidity and permeability of the cell membrane, and the unsaturated degree of the membrane phospholipid is increased and the lipid peroxidation is enhanced, so that the damage of the cell membrane is further aggravated, including the change of the membrane component, the change of the antioxidant function and the change of the membrane permeability, and further the immune function of cells is influenced.

The polyunsaturated fatty acids essential to the human body and involved in the cell membrane construction cannot be metabolized by the human body through other pathways and can only be taken in by diet, so that the polyunsaturated fatty acids ALA, LA, DHA, EPA, phospholipids and antioxidants contained in the composition of the invention in balanced proportion can be supplemented by diet, on one hand, the functional damage such as lipid peroxidation, cell membrane fluidity reduction and deformability reduction caused by the lack or improper proportion of the polyunsaturated fatty acids for constructing cell membranes can be effectively regulated, on the other hand, the Omega-3 fatty acids DHA and EPA combined with the phospholipids provide indispensable constituent components for healthy cell membranes, and meanwhile, the existence of antioxidant substances dispersed in a membrane lipid bilayer also provides antioxidant protection for the Omega-3 fatty acids DHA and EPA, so that the Omega-3 fatty acids DHA and EPA are prevented from being damaged by 'free radicals'. The film antioxidant structurally mixes with the fatty acid to form a protective "first line of defense". The internal structures based on polyunsaturated fatty acid, phospholipid and antioxidant provide synergistic effect for cell membrane homeostasis, namely, triple cell membrane synergistic effect is realized.

Thus, the cell membrane is a healthy protective membrane, and dietary supplementation of the present composition helps to maintain the normal physiological functions of the cell membrane, as well as to maintain the normal immune response and vital activities of the cell.

4. The composition and the proportion of antioxidant substances are further optimized in the composition, wherein the antioxidant substances comprise gamma-tocopherol, alpha-tocopherol and astaxanthin, and the gamma-tocopherol (gamma-tocophenol) and the alpha-tocopherol (alpha-tocophenol) are natural antioxidants and play an important role in antioxidation in a human physiological system in a synergistic manner; astaxanthin is carotenoid with antioxidant property, biologically extracted natural astaxanthin far exceeds artificially synthesized astaxanthin in the aspects of antioxidant property, safety, stability, biological property and the like, has the antioxidant capacity 10 times that of carotene and 100 times that of vitamin E, is called the strongest natural antioxidant found so far, can neutralize free radicals which are unstable molecules, can harm cells and increase the risks of age-related diseases, cancers and heart diseases, and has the functions of antioxidation and anti-aging as a natural antioxidant and can play a positive role in reducing oxidative stress and inflammatory reaction of human bodies; according to the invention, the effects of better synchronously regulating and improving the inflammatory and immune reactions of the human body are further achieved through the optimized composition and proportion of the antioxidant substances.

5. The invention provides a composite oil, which is prepared by mixing hemp seed oil, krill oil and tetrahydrodiphenol in a specific proportion; the hemp seed oil contains more than 80% of polyunsaturated fatty acid, and compared with other vegetable oils, the hemp seed oil is the only oil in which the proportion of Omega-3 alpha-linolenic acid (ALA) is up to 15-30%, and the oil is the natural balanced oil of the proportion of Omega-3 alpha-linolenic acid (ALA) and Omega-6 Linoleic Acid (LA), and can completely meet the healthy intake proportion recommended by the world health organization; the krill oil is rich in Omega-3 fatty acids EPA and DHA, and the addition of the krill oil can reduce the level of endocannabinoid 2-AG which is usually overactive in a chronic inflammatory reaction organism caused by insufficient intake of Omega-3 alpha-linolenic acid, thereby synchronously regulating and improving the inflammatory immune response of a human body caused by endocannabinoid imbalance; the major receptors CB1 and CB2 in the endocannabinoid system (ECS) are triggered by the uptake of exogenous cannabinoid CBD, resulting in a number of different effects, mainly based on the location of the receptors, inhibiting or limiting inflammatory signals of the immune system; by supplementing exogenous cannabinoids, acting synergistically with endogenous cannabinoids, they can act as anti-inflammatory agents to help modulate the immune response, allowing the body to self-heal by better pathways and methods. Meanwhile, the vitamin E in the hemp seed oil is mainly gamma-tocopherol (gamma-tocopherol) and also comprises alpha-tocopherol, wherein the content of the gamma-tocopherol can reach 468 mg/L. Both gamma-tocopherol (gamma-tocophenol) and alpha-tocopherol (alpha-tocophenol) are natural antioxidants, and the two synergistically play an important role in antioxidation in human physiological systems. The natural astaxanthin extracted from organisms is far superior to artificially synthesized astaxanthin in the aspects of antioxidant property, safety, stability, biological property and the like, the antioxidant capacity of the natural astaxanthin is 10 times that of carotene and 100 times that of vitamin E, the natural astaxanthin is called the strongest natural antioxidant found so far, can neutralize free radicals, and the free radicals are unstable molecules, can harm cells and increase the risk of age-related diseases, cancers and heart diseases. Therefore, the hemp seed oil, the krill oil and the tetrahydrodiphenol are matched, so that multiple effects of improving inflammatory physique, improving immune imbalance and improving immunity can be achieved; in addition, the raw material source and the preparation are simpler, the ingredients are purely natural, no western medicine component is contained, the eating method is simple, no education is needed, and the food is easily integrated into daily life.

Detailed Description

Example 1

The composition of a composite oil in kg is shown in the following table 1.

TABLE 1

	Hemp seed oil	Krill oil	Tetrahydrodiphenols
				Example 1	10	6	1
Example 2	20	1	1
				Example 3	15	3	1
Example 4	12	4	1
				Example 5	18	2	1
Comparative example 1	15	3	0
				Comparative example 2	15	0	1
Comparison 3	Walnut oil 15	3	1

In comparative 3 of table 1 above, the hemp oil was replaced with walnut oil. The raw materials are directly mixed and then packaged, the packaging form is light-proof and oxygen-proof (10 g/part), the instant food is very convenient to carry and eat, the eating method is simple, education is not needed, and the daily life can be easily integrated.

Example 2

A composition, comprising: 2.4kg Omega-3 alpha-linolenic acid (ALA), 8.4kg Omega-6 Linoleic Acid (LA) and 1.2kg tetrahydrodiphenol (CBD), and the preparation process comprises the following steps:

1. directly mixing the raw materials until the raw materials are uniform;

2. the package is in a light-proof and oxygen-insulating form (10 g/part), and is convenient to carry and eat instantly.

Example 3

A composition, comprising: 2.23kg ALA, 7.37kg LA, 0.85kg CBD, 0.96kg EPA, 0.56kg DHA, 0.0075kg gamma-tocopherol, 0.0025kg alpha-tocopherol, 0.012kg astaxanthin, its preparation technology is as follows:

1. directly mixing the raw materials until the raw materials are uniform;

Test example 1

Recent researches show that type 2 diabetes, obesity, hyperlipidemia and atherosclerosis are chronic systemic inflammatory diseases, whether the content of Omega-3 polyunsaturated fatty acid and the proportion of Omega-6 linoleic acid and Omega-3 alpha-linolenic acid in the diet are reasonable or not is closely related to the occurrence, development and prevention and treatment of type 2 diabetes, obesity, hyperlipidemia, atherosclerosis and cardiovascular and cerebrovascular diseases.

Homocysteine (HCY) is closely related to dietary structure, and abnormal metabolism of Homocysteine (HCY) can cause abnormal lipid metabolism and result in abnormal increase of Homocysteine (HCY) level in blood, vascular endothelial dysfunction and atherosclerosis; elevated Homocysteine (HCY) concentrations also directly or indirectly cause vascular smooth muscle cell proliferation, platelet aggregation, affect oxidation of low density lipoproteins and promote thrombosis. Homocysteine (HCY) is an independent risk factor for cardiovascular disease and stroke. Studies in the early 90 s of the last century have shown that for men, the risk of myocardial infarction is increased by three times for every 12% increase in the total homocysteine content, and that homocysteine is highly accurate in predicting myocardial infarction, with a stroke risk of 59% for every 5 units increase in homocysteine, and 24% for every three units decrease in stroke risk. Homocysteine is an intermediate product of methionine in human body, and can be regarded as metabolic waste in blood, so that the increase of the index is not only an independent risk factor of cardiovascular and cerebrovascular diseases, but also one of the expressions of the reduction of the metabolic capability of the body. The elevated Homocysteine (HCY) level of a hypertensive is called H-type hypertension, most of the hypertension in China is of the type, the H-type hypertension is very dangerous, the risk of suffering from coronary heart disease and cerebral apoplexy is about 5 times higher than that of a hypertensive patient with normal Homocysteine (HCY), and is about 25-30 times higher than that of a normal person, and the elevated Homocysteine (HCY) level can also increase the risk of suffering from diseases such as arteriosclerosis, venous embolism, kidney diseases, osteoporosis, senile dementia and the like.

According to the clinical guideline for monitoring blood sugar in China, the glycosylated hemoglobin is used as a gold standard for reflecting the long-term blood sugar control level, the glycosylated hemoglobin is a combined product of glucose and hemoglobin, the content of the glycosylated hemoglobin in a body mainly depends on the blood sugar concentration and the contact time of blood sugar and hemoglobin, the formation of the glycosylated hemoglobin is irreversible, and red blood cells have the service life of about 120 days, so that the glycosylated hemoglobin reflects the average blood sugar concentration of the past 8-12 weeks, is hardly interfered by blood drawing time, diet and exercise, and is a relatively stable index by using hypoglycemic drugs and blood sugar fluctuation factors. It is shown that, for every 1% reduction of glycated hemoglobin, the risk of various complications of diabetes, such as microvascular complications, peripheral vascular lesions, myocardial infarction, stroke, etc., is greatly reduced, and the chronic inflammatory condition of the body is beneficially improved.

Fasting plasma glucose, blood fat (triglyceride, low density lipoprotein, total cholesterol) and body fat index (BMI) are all important indexes for evaluating the metabolic capacity changes of organism chronic inflammation level, carbohydrate metabolism, lipid metabolism and the like, are also important indexes closely related to the occurrence and development of cardiovascular and cerebrovascular diseases, have direct/indirect influence relationship with homocysteine and glycosylated hemoglobin, and are also directly influenced by diet and life style, particularly the serious deficiency of Omega-3 alpha-linolenic acid (ALA), Linoleic Acid (LA) and Omega-3 polyunsaturated fatty acid content which lack health proportion in daily edible oil, and are more important reasons for the occurrence and development of the chronic inflammatory diseases.

The specific test process is as follows:

a certain number of healthy subjects with coronary heart disease, blood fat, abnormal blood pressure, type 2 diabetes, obesity and thyroiditis are selected and added into 100 cases of the selected subjects. 48 men, age (58 ± 9) years; women 52 cases, age (60 ± 9). All subjects were evenly divided into 10 groups by health, coronary heart disease, blood lipids, blood pressure abnormalities, type 2 diabetes, obesity, thyroiditis, the 10 groups corresponding to the products prepared in examples 1-3, i.e. 8 groups in example 1, 1 group in example 2 and 1 group in example 3, wherein 8 groups in example 1 correspond to the products of examples 1-5 and comparative examples 1-3, respectively.

Each group of 10 people eat 10g of the product in the corresponding embodiment 1-embodiment 3 according to the grouping method. When each group is eaten for 2 weeks and 4 weeks continuously, 2-3 ml of elbow venous blood is extracted from all the subjects before eating and in the morning on the fasting state on the detection days (2 weeks and 4 weeks), serum is separated to be used for measuring Homocysteine (HCY), fasting plasma glucose (CLU), glycosylated hemoglobin (HbA1c) and blood fat, and Triglyceride (TG), Total Cholesterol (TC) and low-density lipoprotein (LDL) are selected as blood fat indexes. And measuring the fasting body weight and the barefoot height of all the subjects in each group before and after the subjects eat the product, and calculating the Body Mass Index (BMI) of the subjects before and after the subjects eat the product.

The detection results of the parameters are shown in the following table 2, the expression changes of the indexes before and after the subjects eat the food are compared and observed according to the data in the table 2, and the effects of the edible composite oil on the health conditions of the subjects, the related chronic systemic inflammatory diseases and the metabolic disorder conditions are analyzed as shown in the table 3.

TABLE 2 records of related index data before and after each group of edible composite oil

Analysis of the data from Table 2 above corresponds to the data change values shown in Table 3 below.

TABLE 3 variation amplitude of average value of related index before and after eating composite oil (4 weeks) by each group of testers

As can be seen from the data in tables 2-3, the indexes after 4 weeks of continuous eating of the composite oil are reduced and improved to different degrees compared with the original index average of uneaten composite oil, wherein the indexes of the example 1 group, the example 3 group, the example 4 group and the example 3 group are improved most obviously, and the average reduction and improvement amplitude of each group of indexes is higher than that of other groups.

The experimental improvement data shows that the example groups performed better than the control groups overall for the example 1-5 groups compared to the control 1-3 groups. The improvement is more evident in the example 1 group, example 3 and example 4 group. The formula of the group 1-5 is more complete, the proportion of the cannabis oil, the krill oil and the CBD is scientific and reasonable, and the effects of improving the inflammatory constitution, improving the immune imbalance and improving the immunity of the organism of the compound oil composition can be better reflected. In contrast groups 1-3, the ratio of ALA to LA was changed beyond the optimal range, or the content of EPA and DHA was reduced, so the results of the test data showed that the efficacy was inferior to that of the example group, especially the difference was most obvious in contrast group 2. The group of example 2 and the group of example 3 also achieved better results, even the group of example 3 was comparable in index improvement to the group of example 1, the group of example 3 and the group of example 4, but because the components were composed entirely of the corresponding chemical ingredients or of the added ingredients of the dietary supplement, the synergistic effect of some nutrients, enzymes or other active ingredients contained simultaneously in the components of natural plant or animal origin in the group of example 1, the group of example 3 and the group of example 4 could not be compared. And the latter has safer eating quality.

Test example 2

The test uses the products in examples 1 to 3 to verify the change and balance of the anti-inflammatory factor and the proinflammatory factor, and the specific test method, test process and test result are as follows:

the test method comprises the following steps:

the test subjects were the same as in test example 1.

The test process comprises the following steps:

the detection method comprises the following steps: before eating the compound oil product and on the detection day around the edible product, 5ml of elbow venous blood was extracted on an empty stomach by the participants, and the concentrations of IL-6, TNF-alpha, CRP and IL-10 in the serum were measured by ELISA (enzyme-linked immunosorbent assay kit manufactured by R & D, USA). The results are shown in Table 4.

TABLE 4 data records of inflammation indices for each group of testers

TABLE 5 variation of average value of inflammation index after eating composite oil product

From the data in tables 4 and 5 above, it can be seen that: after the products of examples 1-5, comparison 1-3, example 2 and example 3 are continuously eaten for 4 weeks, the test person can inhibit the production of relevant proinflammatory factors such as interleukin-6, tumor necrosis factor (TNF-alpha), C-reactive protein and the like to a certain extent, reduce the level of the proinflammatory factors, and synchronously improve the level of the anti-inflammatory factor IL-10, so that the inflammatory reaction of the body is slowed down. The experimental improvement data demonstrates that the example group performed better than the control group overall compared to the control group. Wherein the inflammation improvement is more obvious in the group of example 1, the group of example 3 and the group of example 4. The formula of the groups 1 to 5 is more complete, and the proportion of the cannabis oil, the krill oil and the CBD is scientific and reasonable, so that the effect of the compound oil composition on improving the inflammatory physique can be better reflected. In the comparison group, because the ratio of ALA to LA is changed beyond the optimal range, or because the content of EPA and DHA is reduced, or because CBD components are absent, the results of the test data show that the efficacy is inferior to that of the example group, especially the difference between the two groups of comparison 1 and 2 is obvious. The group of example 2 and the group of example 3 also achieved better results, even the group of example 3 was comparable in index improvement to the group of example 1, the group of example 3 and the group of example 4, but because the components were composed entirely of the corresponding chemical ingredients or of the added ingredients of the dietary supplement, the synergistic effect of some nutrients, enzymes or other active ingredients contained simultaneously in the components of natural plant or animal origin in the group of example 1, the group of example 3 and the group of example 4 could not be compared. And the latter has safer eating quality.

On the premise of comprehensively considering the efficacy and dosage of each component, the synergistic interaction effect of the dietary supplement food is realized by combining the action mechanism and the correlation relations of mutual synergistic interaction, restriction influence and the like in the human metabolic process and through a precisely proportioned dietary supplement mode, the imbalance of the human immune system is improved, and the inflammatory response of the organism is reduced. The combination of the components of the formula can synchronously adjust the balance of proinflammatory factors and anti-inflammatory factors related to human chronic inflammation through multi-dimension and multi-path, thereby embodying the function of improving the inflammatory response and the immune system efficacy of the organism.

Test example 3

The Omega-3 fatty acid index refers to the percentage of the total concentration of EPA and DHA in the total amount of fatty acids in erythrocytes. The amount of Omega-3 fatty acids in the red cell phospholipids and the amount of Omega-3 fatty acids in the heart are linearly related. By measuring the Omega-3 fatty acid index in red blood cells, the Omega-3 fatty acid content in the heart can be determined, which is an effective tool for analyzing the nutritional status of the human body, especially the heart. When the Omega-3 fatty acid index is less than 4%, the risk of cardiovascular disease increases. The target range of the Omega-3 fatty acid index is 8-11%.

Carotenoids protect epidermal cells from ultraviolet rays and are important indexes of an antioxidant system of an organism. If the test skin has insufficient carotenoid content, it is indicative of a poor body defence. The biological photon scanner is used for detecting body carotenoid by a non-invasive technology, the concentration level of total carotenoid in serum is reflected, and an intuitive antioxidant index can be given. Is the basis for evaluating the health condition and taking effective measures.

This experimental example provides a verification process of the body sub-health condition improvement effect, specifically as follows:

the test process comprises the following steps:

selecting 100 people in sub-health state, 50 men and 50 women; the age is 25-70 years old, and diseases such as chronic diseases and cancers are not diagnosed. The groups were randomized into 10 groups of 10 people each. The products of examples 1 to 3 of the present invention were administered orally at a dose of 10 g/day for 60 days, and the Omega-3 fatty acid index and carotenoid index of the panelists were recorded before and after 60 days.

The detection method comprises the following steps:

1. fingertip blood of a tester before and after the test is respectively collected, and the Omega-3 fatty acid index is detected.

2. The carotenoid index in skin was detected using a biophotonic scanner.

The results are shown in Table 6.

TABLE 6 data record of the Omega-3 fatty acid index and carotenoid index of the testers

From the data in table 6 above, it can be seen that: after 60 days of administration of the test product containing a higher content of EPA and DHA, the Omega-3 concentration in the blood can be increased more effectively than with the lower content of the test product. Wherein the example 1 group, the example 3 group, the example 4 group and the example 3 group increase by 4 and above and reach or approach the target range (8-11%) of index adjustment. While the other groups had an amplification of 4 or less and did not reach the target range. The magnitude of the difference between the highest and lowest improvement changes was significantly different (example 3 group/9 versus comparative 2 group/4).

Meanwhile, the indexes of the skin carotenoids of each group reflecting the oxidation resistance level of the human body are correspondingly improved, the improvement range is larger when the test group containing high EPA and DHA is taken, the improvement ranges of the example 1 group, the example 4 group and the example 3 group are close to or more than ten thousand, and the original common level (less than 3 ten thousand) lower than the common carotenoid index reaches or is close to the common level index. The other groups had improvements of 8000 or less. The highest amplitude of improvement was significantly different from the lowest amplitude (example 1 group/10276 versus comparative 2 group/2029). The food supplement of high EPA and DHA contents has certain promotion effect on improving the oxidation resistance of human bodies and improving relevant indexes of human health.

It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.

Claims

1. The composition is characterized by comprising LA, ALA and CBD, wherein the mass ratio of the LA to the ALA to the CBD is (7-9) to (2-3) to 1.

2. The composition according to claim 1, further comprising EPA, DHA and an antioxidant, wherein the weight ratio of EPA, DHA, CBD and antioxidant is (43-45): 25-26): 38-39): 1.

3. The composition according to claim 1 or 2, wherein the antioxidant substance is gamma-tocopherol, alpha-tocopherol and astaxanthin, wherein the mass ratio of the alpha-tocopherol to the gamma-tocopherol to the astaxanthin is 1 (2-4) to (4-5).

4. The compound oil is characterized by comprising hemp seed oil, krill oil and tetrahydrodiphenol, wherein the mass ratio of the hemp seed oil to the krill oil to the tetrahydrodiphenol is (10-20) to (1-6) to 1.

5. Use of a composition according to any one of claims 1 to 3 or a complex oil according to claim 4 in a product for ameliorating an imbalance in the immune system of a human and/or a predisposition for chronic inflammation.

6. Use according to claim 5, characterized in that the imbalance of the human immune system or/and the constitution of chronic inflammation is improved by inhibiting chronic inflammation of the body.

7. The use as claimed in claim 6, wherein the imbalance of the human immune system or/and the predisposition to chronic inflammation is ameliorated by regulating the balance of the human essential fatty acids and the endocannabinoid system.

8. The use as claimed in claim 7, wherein the oxidative stress and inflammatory response in humans is improved by modulating the levels of inflammatory factors, and modulating the overall level of the endocannabinoid AEA.

9. The use according to claim 8, characterized in that the level of the anti-inflammatory factor IL-10 is increased by decreasing the level of the pro-inflammatory factors IL-6, TNF- α, C-reactive protein related cytokines, thereby improving the oxidative stress and inflammatory response of the human body.

10. The use according to any one of claims 6 to 9, wherein the risk of cardiovascular and cerebrovascular diseases and cancer is reduced by improving the constitution of chronic inflammation, the symptoms of chronic inflammation such as hyperlipidemia, hyperglycemia, cholesterol abnormality and obesity are improved, the health of skin is promoted, hypothyroidism and endocrine dyscrasia are improved, mental and nervous system disorders are alleviated, chronic pain is alleviated, the health of brain is protected, autoimmune diseases are improved, metabolic syndrome is improved, and hyperactivity is improved.