CN112684192A - New application of ammonium bradykinin in ABO blood group reverse typing kit - Google Patents
New application of ammonium bradykinin in ABO blood group reverse typing kit Download PDFInfo
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- CN112684192A CN112684192A CN202011558889.7A CN202011558889A CN112684192A CN 112684192 A CN112684192 A CN 112684192A CN 202011558889 A CN202011558889 A CN 202011558889A CN 112684192 A CN112684192 A CN 112684192A
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Abstract
The invention discloses a new application of ammonium bradykinin in an ABO blood group reverse typing kit, belonging to the technical field of blood cell analysis, and the technical scheme of the invention is as follows: 31.9g of glucose, 2.22g of sodium dihydrogen phosphate, 12.57g of mannitol, 0.275g of adenine, 26.3g of sodium citrate, 3.27g of citric acid, 2.0g of ammonium bradykinin, 15ml of glycerol, 0.5g of promethazine and preservative Proclin-300200 mu L are accurately weighed and dissolved in 1L of distilled water, 3.97g of sodium chloride is used for adjusting osmotic pressure to 300mosm/kg. H2O, adjusting the pH value to be within 7.35-7.50, and respectively adding 4mL of standard A1B, O form an ABO blood group reverse typing reagent containing ammonium bradygenate.
Description
Technical Field
The invention belongs to the technical field of blood analysis; or a method for testing materials by using visible light and generating color change through the result of test reaction, in particular to a method for detecting by using ABO blood group reverse typing reagent containing ammonium buffer.
Background
The main component of ammonium tromethamine is tromethamine, which is clinically used for acute metabolic and respiratory acidemia, is an amino acid buffer, can absorb hydrogen ions to correct acidosis, has strong effect, can pass through cell membranes, and can correct acidosis inside and outside cells simultaneously. Can be used for metabolic and respiratory acidosis, and can also be used for cardiac resuscitation, and mixed acidosis is often caused when cardiac respiration stops.
Ammonium buffered carbonate is commonly used for preparing a Siemens hematometer analyzer sheath fluid in medical inspection, when the sheath fluid is prepared, a reagent and water with higher purity are selected, the pH value of the sheath fluid is adjusted to be within the range of 7.3-7.5, the ammonium buffered carbonate is a buffer solution, the pH value of the sheath fluid is maintained, and the buffer solution is a main component of the sheath fluid.
Ammonium bradykinin in the present invention is primarily responsible for increasing reactivity of erythrocyte surface antigens, ammonium bradykinin is standard erythrocyte A1B, O is changed into spherical shape, which increases the reactivity of the surface antigen of erythrocyte membrane and makes the surface antigen of erythrocyte membrane fully react.
Disclosure of Invention
The invention aims to provide a method for performing reverse typing detection by using ABO blood type reverse typing reagent containing ammonium tromethate, and standard red blood cell A in a kit1B, O is dissolved in a health liquid containing ammonium tromethate, citric acid, citrate, sodium dihydrogen phosphate, glucose, adenine, sodium chloride, glycerol, mannitol, promethazine, etc.
The technical scheme is as follows: increasing reactivity of erythrocyte surface antigen in ABO blood group reverse typing kit by ammonium buffered sulfate1B, O is changed into spherical shape, which increases reactivity of erythrocyte membrane surface antigen, citrate is basic anticoagulant substance in all anticoagulant preservation solution, which can prevent hemolysis; the citric acid avoids the nutrient components in the preservation solution which are necessary for red blood cell metabolism, can prolong the preservation time of red blood cells, prevent hemolysis, slow down the disappearance of organic phosphorus in the cells and prevent the damage of red blood cells during storage; adenine can promote synthesis of erythrocyte ATP and prolong preservation period of erythrocyteAnd enhance the oxygen release function of the red blood cells; the phosphate improves the pH value of the preservation solution and prolongs the preservation period of the red blood cells; sodium chloride regulates the osmotic pressure of erythrocytes; glycerol and mannitol are capable of maintaining the integrity of the erythrocyte membrane; promethazine can slow down red blood cell metabolism; proclin-300 is a preservative.
Drawings
FIG. 1 is a schematic diagram of the ABO blood group reverse typing kit containing ammonium bradykinin according to the present invention;
in the figure: 1. box body 2. box cover
3. Groove 4.A1Counter-committed cells
B-negative committed cell 6. O-negative committed cell
Detailed Description
Example 1
Preparation of reagents: accurately weighing glucose (C)6H12O6·H2O)31.9g, sodium dihydrogen phosphate (NaH)2PO4·2H2O)2.22g, mannitol (C)6H14O6)12.57g, adenine (C)5H5N5)0.275g, sodium citrate (C)6H5O7Na3·2H2O)26.3g, citric acid (C)6H8O7·H2O)3.27g, ammonium bradykinin 2.0g, glycerin 15ml, promethazine 0.5g, preservative Proclin-300200 μ L dissolved in 1L distilled water, sodium chloride (NaCl)3.97g was used to adjust the osmotic pressure to 300mosm/(kg. H.2O), adjusting the pH value to be within 7.35-7.50, and respectively adding 4mL of standard A1B, O, forming an ABO blood type reverse sizing reagent, storing in a refrigerator at 2-8 ℃, and the structure of the kit is shown in figure 1.
Example 2
A detection step: taking two test tubes, marking, and adding 2 drops of serum (blood plasma) to be detected in each test tube; adding 1 drop of 4% standard A1 and B, O reagent erythrocyte suspension into the labeled test tube; gently mixing the contents of the test tube, and centrifuging the contents for 15s at 1000g by using a special centrifuge for blood group serology; taking the test tube out of the centrifuge, holding the test tube at an acute angle, slowly inclining to enable the liquid to repeatedly wash the erythrocyte buttons, continuously slowly inclining and shaking when the erythrocytes are not attached to the wall of the test tube any more, and observing whether uniform erythrocyte suspension or agglutination is formed in the test tube; suspected of weak agglutination, the sample should be transferred to a microscope for observation, and the observed agglutination intensity recorded for mutual verification with the positive typing results.
The ABO blood group reverse typing kit of the present invention for enhancing erythrocyte blood group antigen reactivity with ammonium bradykinin is within the scope of the present invention, and any changes or substitutions that can be made by one skilled in the art without creative efforts are within the technical scope of the present invention.
Claims (5)
1. The invention discloses a new application of ammonium tromethate in an ABO blood group reverse typing kit, which belongs to the technical field of blood cell analysis and is technically characterized in that a method for detecting the ABO blood group reverse typing reagent containing the ammonium tromethate is adopted, and standard erythrocytes A1 and B, O in the kit are dissolved in a maintenance solution containing the ammonium tromethate, citric acid, citrate, sodium dihydrogen phosphate, glucose, adenine, sodium chloride, glycerol, mannitol, promethazine and the like.
2. The new use of ammonium hypoxelate in ABO blood group reverse typing kit according to claim 1, wherein each liter of the maintenance liquid contains 20.0-40.0 g of glucose, 1.20-3.20 g of sodium dihydrogen phosphate, 6.0-18.0 g of mannitol, 0.175-0.375 g of adenine, 16.0-36.0 g of sodium citrate, 2.27-4.27 g of citric acid, 1.0-3.0 g of ammonium hypoxelate, 5.0-25.0 mL of glycerol, 0.30-0.70 g of promethazine, and 3.97g of sodium chloride, and 4mL of A is added1B, O form an ABO blood group reverse typing reagent.
3. The new use of ammonium tromethate in ABO blood group reverse typing kit according to claim 1, characterized in that the osmotic pressure of the reagent is 275-305 mosm/(kg.H)2O)。
4. The new use of ammonium bradykinin in ABO blood group reverse typing kit according to claim 1, wherein the pH value of the reagent is 7.35-7.50.
5. The new use of ammonium bradykinin in ABO blood group reverse typing kit according to claim 1, wherein the final concentration of A1, B, O reagent erythrocyte suspension is 3-5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011558889.7A CN112684192A (en) | 2020-12-25 | 2020-12-25 | New application of ammonium bradykinin in ABO blood group reverse typing kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011558889.7A CN112684192A (en) | 2020-12-25 | 2020-12-25 | New application of ammonium bradykinin in ABO blood group reverse typing kit |
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| CN112684192A true CN112684192A (en) | 2021-04-20 |
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| CN202011558889.7A Pending CN112684192A (en) | 2020-12-25 | 2020-12-25 | New application of ammonium bradykinin in ABO blood group reverse typing kit |
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1924583A (en) * | 2006-08-02 | 2007-03-07 | 北京金豪制药有限公司 | ABO blood group inverse reagent |
| CN2901313Y (en) * | 2006-04-24 | 2007-05-16 | 中国人民解放军军事医学科学院基础医学研究所 | ABO blood group anti-fixation reagent kit |
| CN101101293A (en) * | 2006-06-22 | 2008-01-09 | 基立福有限公司 | Suspension medium for red blood cells |
| CN101178410A (en) * | 2006-11-07 | 2008-05-14 | 上海血液生物医药有限责任公司 | Red corpuscle reagent box for reverse typing used for detecting human ABO blood type |
| CN102338800A (en) * | 2011-05-25 | 2012-02-01 | 上海血液生物医药有限责任公司 | Antibody screening erythrocyte kit |
| CN102466730A (en) * | 2010-11-19 | 2012-05-23 | 南京神州英诺华医疗科技有限公司 | Novel method for matching blood reverse typing reagent |
| CN104304235A (en) * | 2014-09-27 | 2015-01-28 | 何永勋 | A reagent erythrocyte preserving fluid and a preparing method thereof |
| CN105334191A (en) * | 2014-07-25 | 2016-02-17 | 深圳迈瑞生物医疗电子股份有限公司 | Hemoglobin concentration and volume correction method and apparatus for individual erythrocyte |
| CN109566602A (en) * | 2019-01-04 | 2019-04-05 | 宁波艾捷康宁生物科技有限公司 | A kind of alserver's solution and save set |
| CN111713487A (en) * | 2020-06-30 | 2020-09-29 | 上海市血液中心 | Reagent erythrocyte preservation system and preparation method thereof |
-
2020
- 2020-12-25 CN CN202011558889.7A patent/CN112684192A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2901313Y (en) * | 2006-04-24 | 2007-05-16 | 中国人民解放军军事医学科学院基础医学研究所 | ABO blood group anti-fixation reagent kit |
| CN101101293A (en) * | 2006-06-22 | 2008-01-09 | 基立福有限公司 | Suspension medium for red blood cells |
| CN1924583A (en) * | 2006-08-02 | 2007-03-07 | 北京金豪制药有限公司 | ABO blood group inverse reagent |
| CN101178410A (en) * | 2006-11-07 | 2008-05-14 | 上海血液生物医药有限责任公司 | Red corpuscle reagent box for reverse typing used for detecting human ABO blood type |
| CN102466730A (en) * | 2010-11-19 | 2012-05-23 | 南京神州英诺华医疗科技有限公司 | Novel method for matching blood reverse typing reagent |
| CN102338800A (en) * | 2011-05-25 | 2012-02-01 | 上海血液生物医药有限责任公司 | Antibody screening erythrocyte kit |
| CN105334191A (en) * | 2014-07-25 | 2016-02-17 | 深圳迈瑞生物医疗电子股份有限公司 | Hemoglobin concentration and volume correction method and apparatus for individual erythrocyte |
| CN104304235A (en) * | 2014-09-27 | 2015-01-28 | 何永勋 | A reagent erythrocyte preserving fluid and a preparing method thereof |
| CN109566602A (en) * | 2019-01-04 | 2019-04-05 | 宁波艾捷康宁生物科技有限公司 | A kind of alserver's solution and save set |
| CN111713487A (en) * | 2020-06-30 | 2020-09-29 | 上海市血液中心 | Reagent erythrocyte preservation system and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 赵春亭 等: "《临床血液流变学》", 人民卫生出版社, pages: 11 - 12 * |
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