CN112666319A - Nitrilase enzyme property research comparison device and method based on cloud database - Google Patents
Nitrilase enzyme property research comparison device and method based on cloud database Download PDFInfo
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- CN112666319A CN112666319A CN202011515983.4A CN202011515983A CN112666319A CN 112666319 A CN112666319 A CN 112666319A CN 202011515983 A CN202011515983 A CN 202011515983A CN 112666319 A CN112666319 A CN 112666319A
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Abstract
The invention discloses nitrilase enzyme property research comparison equipment and a nitrilase enzyme property research comparison method based on a cloud database, and belongs to the technical field of nitrilase enzyme property research. The nitrilase property research comparison device based on the cloud database and the using method thereof improve the detection efficiency and speed, facilitate continuous detection, increase the detection efficiency, ensure the detection quality, improve the quality inspection accuracy, facilitate the comparison of multiple groups of data, improve the authenticity and reliability of comparison results, compare from big data, increase the efficiency of nitrilase liquid comparison, and prevent detection beaker dislocation from causing detection failure.
Description
Technical Field
The invention relates to the technical field of nitrilase enzyme property research, in particular to nitrilase enzyme property research comparison equipment and method based on a cloud database.
Background
Nitriles are a class of organic compounds containing-CN groups, and are starting materials for the synthesis of amides, carboxylic acids, esters, and the like. They are not only of a wide variety but also widely distributed in nature, such as acetonitrile used as a solvent, monomeric acrylonitrile of high polymers, bromoxynil and ioxynil insecticides, ricinine produced in microorganisms and plants, and the like. Most nitrile compounds have toxicity, teratogenicity and carcinogenicity, but carboxylic acid obtained by hydrolysis of the nitrile compounds is widely applied to fine chemical engineering, medical intermediates, vitamin precursors and the like, and has higher value-added value. The chemical hydrolysis reaction conditions of nitrile are harsh (high temperature, high pressure, strong acid or strong base) and are accompanied with the formation of a large amount of salts, which brings difficulty for subsequent separation and purification and also causes certain environmental pollution, so the industrial application has certain limitation, the quality of the existing nitrilase is detected after the production, most of nitrilases only aim at single nitrilase for detection, the nitrilase quality and quality cannot be compared according to the situation, and the nitrilase can not be screened and better produced.
Disclosure of Invention
The invention aims to provide a nitrilase property research and comparison device and a nitrilase property research and comparison method based on a cloud database, which improve the detection efficiency and speed, can simultaneously detect a plurality of groups of data, can install upper threads of a detection probe, is convenient to disassemble and replace, increases the use convenience, is convenient to detect continuously, increases the detection efficiency, also ensures the detection quality, improves the accuracy of quality inspection, is convenient to compare a plurality of groups of data, improves the authenticity and reliability of comparison results, compares big data, increases the efficiency of comparing nitrilase liquid, also is convenient to transmit the detected nitrilase liquid detection results to a storage unit and the cloud database for storage so as to be convenient for next comparison and lookup, prevents detection beaker dislocation and detection failure, reduces dust entering in the detection process of a housing, increases the detection accuracy, facilitates the detection of the detection beaker in and out of the slot, to solve the problems set forth in the background art described above.
In order to achieve the purpose, the invention provides the following technical scheme:
equipment is compared in nitrilase enzyme property research based on cloud database, including the workstation, the side of workstation sets up the support column, and check out test set is installed to the top of support column, and the comparison device is connected to check out test set's top, and check out test set is connected with the comparison device electricity, set up transmission device on the workstation, transmission device's top sets up the dustcoat.
Further, the detection equipment comprises a detection box, a telescopic device, a detection probe, a detector and a cleaning device, wherein the detector is arranged inside the detection box, the detection probe is connected below the detector, the upper part of the detection probe is connected with the detection box through the telescopic device, the cleaning device is arranged below the detection box, and the detection probe is provided with a plurality of parts which are all fixed below the detection box through the telescopic device.
Further, the telescoping device includes telescopic cylinder, telescopic link, mounting panel and fixed sleeving, and the telescopic link is connected to the output of telescopic cylinder below, and the mounting panel is connected to the below of telescopic link, a plurality of fixed sleeving of below installation of mounting panel, sets up the internal thread in fixed sleeving's the below connector, and it is solid in fixed sleeving to twist through the screw thread in the upper end of test probe.
Further, cleaning device includes mounting bracket, the interior lantern ring, disinfection layer and clearance layer, and the mounting bracket upper end is fixed in the detection case below, and the lantern ring in the below of mounting bracket sets up disinfection layer and clearance layer in the interior lantern ring, and interior lantern ring, disinfection layer and clearance layer set up the multiunit, and is the same with test probe quantity.
Further, the comparison device comprises a cloud database, a retrieval unit, a comparison unit, a collection unit, a storage unit and a server, the cloud database and the storage unit are arranged in the server, the storage unit is electrically connected with the collection unit, the collection unit is electrically connected with the comparison unit, the comparison unit is electrically connected with the retrieval unit, and the collection unit is electrically connected with the detection equipment.
Further, transmission device cup joints the pivot pole including cup jointing roller, pivot pole, rotation motor and conveyer belt, cup jointing the inside cup joint pivot pole of roller, and the one end of pivot pole and the output shaft who rotates the motor cup joint the roller and set up two sets ofly, cup joints and cup joints by the conveyer belt between the roller, offers the mounting groove on the conveyer belt, sets up on the mounting groove and detects the beaker.
Furthermore, the outer cover is fixed above the workbench, and the two ends of the outer cover are provided with an inlet and outlet groove.
The invention provides another technical scheme: a method for using equipment for researching and comparing nitrilase enzyme properties based on a cloud database comprises the following steps:
the method comprises the following steps: placing the detection beakers filled with different nitrilase liquids on a mounting groove on a conveyer belt, starting the conveyer belt for transportation, and driving the detection beakers to move below the detection equipment;
step two: the telescopic device drives the detection probe to move downwards, the detection probe is pressed into the detection beaker to perform quality detection on different nitrilase liquids, and the detection probe transmits a detected structure into the detector;
step three: the detector transmits the detected information to the comparison device, the collection unit collects and combs the information, the quality inspection information is transmitted to the comparison unit, and a cloud database is used for comparing multiple groups of data;
step four: when the detecting probe detects next time, the telescopic device drives the detecting probe to move upwards, the detecting probe retracts into the inner sleeve ring, and the disinfecting layer and the cleaning layer disinfect and clean the detecting probe, so that the detecting probe can detect next time conveniently.
Further, in the step one, an infrared sensor is arranged below the detection box and used for detecting the beaker to be positioned.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the device and the method for researching and comparing the nitrilase properties based on the cloud database, the plurality of fixing sleeves are installed below the mounting plate, internal threads are arranged in the lower connectors of the fixing sleeves, the upper ends of the detection probes are screwed in the fixing sleeves through the threads, the telescopic device drives the detection probes to move downwards, the detection probes are pressed into the detection beaker to perform quality inspection on different nitrilase liquids, the detection probes transmit detected structures into the detector, the detection probes are arranged to detect simultaneously, the detection efficiency and speed are improved, multiple groups of data can be detected simultaneously, the threads above the detection probes are installed, disassembly and replacement are convenient, and the use convenience is improved.
2. The invention provides a device and a method for researching and comparing nitrilase properties based on a cloud database.
3. The invention provides equipment and a method for researching and comparing nitrilase properties based on a cloud database.
4. According to the device and the method for researching and comparing the nitrilase properties based on the cloud database, the detection beakers filled with different nitrilase liquids are placed on the mounting grooves in the conveying belt, the conveying belt is started to transport the nitrilase liquids, the detection beakers are driven to move to the positions below the detection device, the mounting grooves increase the stability of the detection beakers when the detection beakers are placed and then move, the infrared sensors are used for conducting infrared sensing positioning on the detection beakers on the conveying belt, the detection beakers are prevented from being misplaced and causing detection failure, the outer cover reduces dust entering in the detection process, the detection accuracy is increased, and the detection beakers can be conveniently transported back and forth through the inlet and outlet grooves.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the internal structure of the housing of the present invention;
FIG. 3 is a cross-sectional view of the structure of the detecting device of the present invention;
FIG. 4 is a schematic structural diagram of the inspection apparatus of the present invention;
FIG. 5 is a schematic view of the structure of the telescopic device of the present invention;
FIG. 6 is a schematic structural diagram of a cleaning apparatus according to the present invention;
FIG. 7 is an enlarged view taken at A of FIG. 3 according to the present invention;
FIG. 8 is a block diagram of a connection module of the alignment apparatus according to the present invention;
fig. 9 is a schematic view of a detachable structure of the transmission device and the housing of the present invention.
In the figure: 1. a work table; 11. a support pillar; 2. a detection device; 21. a detection box; 211. an infrared sensor; 22. a telescoping device; 221. a telescopic cylinder; 222. a telescopic rod; 223. mounting a plate; 224. fixing the sleeve; 23. detecting a probe; 24. a detector; 25. a cleaning device; 251. a mounting frame; 252. an inner collar; 253. a disinfection layer; 254. cleaning the layer; 3. a comparison device; 31. a cloud database; 32. a retrieval unit; 33. a comparison unit; 34. a collection unit; 35. a storage unit; 36. a server; 4. a transmission device; 41. sleeving a roll shaft; 42. a spindle rod; 43. rotating the motor; 44. a conveyor belt; 441. mounting grooves; 442. detecting the beaker; 5. a housing; 51. and (4) entering and exiting the groove.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-2, the apparatus and method for comparing nitrilase enzyme properties based on cloud database comprises a workbench 1, a support column 11 is arranged at a side end of the workbench 1, a detection apparatus 2 is arranged above the support column 11, a comparison device 3 is connected above the detection apparatus 2, the detection apparatus 2 is electrically connected with the comparison device 3, a transmission device 4 is arranged on the workbench 1, and a housing 5 is arranged above the transmission device 4.
Referring to fig. 3-5, the detecting apparatus 2 includes a detecting box 21, a telescopic device 22, a detecting probe 23, a detector 24 and a cleaning device 25, the detector 24 is disposed inside the detecting box 21, the detecting probe 23 is connected below the detector 24, the detecting probe 23 is connected with the detecting box 21 through the telescopic device 22, the cleaning device 25 is disposed below the detecting box 21, the detecting probe 23 is provided with a plurality of telescopic devices 22, the telescopic devices 22 are all fixed below the detecting box 21, each telescopic device 22 includes a telescopic cylinder 221, a telescopic rod 222, a mounting plate 223 and a fixed sleeve 224, an output end below the telescopic cylinder 221 is connected with the telescopic rod 222, the mounting plate 223 is connected below the telescopic rod 222, a plurality of fixed sleeves 224 are mounted below the mounting plate 223, an internal thread is disposed in a lower connecting port of the fixed sleeve 224, an upper end of the detecting probe 23 is screwed into the fixed sleeve 224 through a thread, telescoping device 22 drives test probe 23 and moves down, test probe 23 impresses and carries out the quality control to different nitrilase liquid in detecting beaker 442, and test probe 23 transmits the structure that detects to detector 24 in, detects simultaneously through setting up a plurality of test probe 23, improves detection efficiency and speed, can detect multiunit data simultaneously, and test probe 23's top screw thread is installed, convenient to detach and change, increase and use the convenience.
Referring to fig. 6-7, the cleaning device 25 includes a mounting frame 251, an inner collar 252, a disinfection layer 253 and a cleaning layer 254, the upper end of the mounting frame 251 is fixed below the detection box 21, the inner collar 252 is disposed below the mounting frame 251, the disinfection layer 253 and the cleaning layer 254 are disposed in the inner collar 252, the disinfection layer 253 and the cleaning layer 254 are provided with multiple sets, the number of the sets is the same as that of the detection probes 23, when the detection probes 23 perform the next detection, the telescopic device 22 drives the detection probes 23 to move upwards, the detection probes 23 retract into the inner collar 252, the disinfection layer 253 and the cleaning layer 254 perform disinfection and cleaning, the telescopic device 22 drives the detection probes 23 to move downwards again, quality inspection is performed on different nitrilase liquids in the next detection beaker 442, so as to facilitate continuous detection, increase detection efficiency, ensure detection quality, and improve accuracy of quality inspection.
Referring to fig. 8, the comparison device 3 includes a cloud database 31, a retrieval unit 32, a comparison unit 33, a collection unit 34, a storage unit 35 and a server 36, the cloud database 31 and the storage unit 35 are disposed in the server 36, the storage unit 35 is electrically connected with the collection unit 34, the collection unit 34 is electrically connected with the comparison unit 33, the comparison unit 33 is electrically connected with the retrieval unit 32, the collection unit 34 is electrically connected with the detection device 2, the detector 24 transmits the detected information to the comparison device 3, the collection unit 34 collects and combs the information, transmits the quality inspection information to the comparison unit 33, the cloud database 31 is used for comparing multiple sets of data to obtain nitrilase liquid with good quality, the server 36 is used for accessing cloud data, so as to retrieve the detection result of the nitrilase liquid from the internet, facilitate comparison of multiple sets of data, and improve the authenticity and reliability of the comparison result, the big data are compared, the efficiency of comparing the nitrilase liquid is improved, and the detected nitrilase liquid detection result is conveniently transmitted to the storage unit 35 and the cloud database 31 to be stored so as to facilitate the comparison and reference in the next time.
Referring to fig. 9, the conveying device 4 includes a sleeve-connection roller shaft 41, a rotating shaft 42, two sets of rotating motors 43 and a conveying belt 44, the inner portion of the sleeve-connection roller shaft 41 is sleeved with the rotating shaft 42, one end of the rotating shaft 42 is connected with an output shaft of the rotating motor 43, the sleeve-connection roller shafts 41 are sleeved with each other by the conveying belt 44, the conveying belt 44 is provided with a mounting groove 441, the mounting groove 441 is provided with a detection beaker 442, the cover 5 is fixed above the workbench 1, two ends of the cover 5 are provided with an inlet and outlet groove 51, an infrared sensor 211 is arranged below the detection box 21, the infrared sensor 211 is used for positioning the detection beaker 442, the detection beaker 442 containing different nitrilase liquids is placed on the mounting groove 441 on the conveying belt 44, the conveying belt 44 is started for transportation to drive the detection beaker 442 to move below the detection device 2, the mounting groove 441 increases the stability of the detection beaker 442 when moving after being placed, infrared inductor 211 is used for carrying out the infrared induction location to the beaker 442 that detects on the conveyer belt 44, prevents to detect the beaker 442 dislocation, leads to detecting the failure, and dustcoat 5 reduces the dust and gets into in the testing process, increases and detects the precision, and the groove 51 of business turn over is convenient for detect the transport of beaker 442 round trip.
In order to better show the research comparison process of the nitrilase enzyme property research comparison equipment based on the cloud database, the embodiment provides a method for research comparison of the nitrilase enzyme property research comparison equipment based on the cloud database, which comprises the following steps:
the method comprises the following steps: placing the detection beakers 442 filled with different nitrilase liquids on the mounting groove 441 on the conveyer belt 44, starting the conveyer belt 44 for transportation, and driving the detection beakers 442 to move below the detection device 2;
step two: the telescopic device 22 drives the detection probe 23 to move downwards, the detection probe 23 is pressed into the detection beaker 442 to carry out quality detection on different nitrilase liquids, and the detection probe 23 transmits a detected structure into the detector 24;
step three: the detector 24 transmits the detected information to the comparison device 3, the collection unit 34 collects and combs the information, the quality inspection information is transmitted to the comparison unit 33, and a plurality of groups of data are compared through the cloud database 31;
step four: when the detection probe 23 is used for the next detection, the telescopic device 22 drives the detection probe 23 to move upwards, the detection probe 23 retracts into the inner lantern ring 252, and the disinfection layer 253 and the cleaning layer 254 are used for disinfection and cleaning, so that the next detection is facilitated.
In summary, according to the apparatus and method for researching and comparing nitrilase properties based on cloud database provided by the present invention, a plurality of fixed sleeves 224 are installed below a mounting plate 223, an internal thread is provided in a lower connection port of the fixed sleeves 224, the upper end of a detection probe 23 is screwed in the fixed sleeves 224 through a thread, a telescopic device 22 drives the detection probe 23 to move down, the detection probe 23 is pressed into a detection beaker 442 to perform quality detection on different nitrilase liquids, the detection probe 23 transmits the detected structure into a detector 24, the detection is performed simultaneously by arranging a plurality of detection probes 23, so as to improve the detection efficiency and speed, and can simultaneously detect a plurality of sets of data, the upper thread of the detection probe 23 is installed, so as to facilitate disassembly and replacement, and increase the convenience in use, the inner sleeve 252, the disinfection layer 253 and the cleaning layer 254 are provided with a plurality of sets, the number of the sets is the same as that of the detection probes 23, when the detection probes 23 perform the, the telescopic device 22 drives the detection probe 23 to move upwards, the detection probe 23 retracts into the inner lantern ring 252, the sterilization layer 253 and the cleaning layer 254 are used for sterilization and cleaning, the telescopic device 22 drives the detection probe 23 to move downwards again, quality inspection is carried out on different nitrilase liquids in the next batch of detection beakers 442, continuity detection is facilitated, detection efficiency is improved, detection quality is guaranteed, accuracy of quality inspection is improved, the collection unit 34 collects and combs information, the quality inspection information is transmitted to the comparison unit 33, multiple groups of data are compared through the cloud database 31, nitrilase liquids with good quality are obtained, cloud data are accessed through the server 36, nitrilase liquid detection results can be retrieved from the internet conveniently, multiple groups of data comparison is facilitated, authenticity and reliability of comparison results are improved, comparison is carried out through the big data, and nitrilase liquid comparison efficiency is improved, also be convenient for store in transmitting the nitrilase liquid detection achievement after detecting to memory cell 35 and cloud database 31 so that compare next time and consult, place the detection beaker 442 that will be equipped with different nitrilase liquid on the mounting groove 441 on conveyer belt 44, conveyer belt 44 starts to transport, drive and detect beaker 442 and move the below to check out device 2, mounting groove 441 increases the stability when moving after placing detection beaker 442, infrared inductor 211 is used for carrying out the infrared induction location to detection beaker 442 on conveyer belt 44, prevent to detect beaker 442 dislocation, lead to detecting the failure, dustcoat 5 is in the testing process, reduce the dust and get into, increase and detect the precision, it transports back and forth to go out groove 51 to be convenient for detect beaker 442.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.
Claims (9)
1. Nitrilase enzyme property research compares equipment based on cloud database includes workstation (1), its characterized in that: the side of workstation (1) sets up support column (11), and check out test set (2) are installed to the top of support column (11), and device (3) is compared in the top connection of check out test set (2), check out test set (2) with compare device (3) electricity and be connected, set up transmission device (4) on workstation (1), the top of transmission device (4) sets up dustcoat (5).
2. The cloud database-based nitrilase enzyme property research alignment apparatus of claim 1, wherein: the detection device (2) comprises a detection box (21), a telescopic device (22), a detection probe (23), a detector (24) and a cleaning device (25), the detector (24) is arranged inside the detection box (21), the detection probe (23) is connected below the detector (24), the telescopic device (22) is connected with the detection box (21) above the detection probe (23), the cleaning device (25) is arranged below the detection box (21), and the detection probe (23) is provided with a plurality of telescopic devices (22) which are all fixed below the detection box (21).
3. The cloud database-based nitrilase enzyme property research alignment apparatus of claim 2, wherein: the telescopic device (22) comprises a telescopic cylinder (221), a telescopic rod (222), a mounting plate (223) and a fixing sleeve (224), the telescopic rod (222) is connected to the output end below the telescopic cylinder (221), the mounting plate (223) is connected to the lower portion of the telescopic rod (222), a plurality of fixing sleeves (224) are mounted below the mounting plate (223), internal threads are arranged in a connector below the fixing sleeve (224), and the upper end of the detection probe (23) is screwed in the fixing sleeve (224) through the threads.
4. The cloud database-based nitrilase enzyme property research alignment apparatus of claim 2, wherein: cleaning device (25) include mounting bracket (251), interior lantern ring (252), disinfection layer (253) and clearance layer (254), and mounting bracket (251) upper end is fixed in detection case (21) below, and lantern ring (252) in the below setting of mounting bracket (251), sets up disinfection layer (253) and clearance layer (254) in interior lantern ring (252), and interior lantern ring (252), disinfection layer (253) and clearance layer (254) set up the multiunit, and is the same with test probe (23) quantity.
5. The cloud database-based nitrilase enzyme property research alignment apparatus of claim 1, wherein: the comparison device (3) comprises a cloud database (31), a retrieval unit (32), a comparison unit (33), a collection unit (34), a storage unit (35) and a server (36), the cloud database (31) and the storage unit (35) are arranged in the server (36), the storage unit (35) is electrically connected with the collection unit (34), the collection unit (34) is electrically connected with the comparison unit (33), the comparison unit (33) is electrically connected with the retrieval unit (32), and the collection unit (34) is electrically connected with the detection equipment (2).
6. The cloud database-based nitrilase enzyme property research alignment apparatus of claim 1, wherein: the conveying device (4) comprises a sleeve connection roller shaft (41), a rotating shaft rod (42), a rotating motor (43) and a conveying belt (44), the rotating shaft rod (42) is sleeved in the sleeve connection roller shaft (41), one end of the rotating shaft rod (42) is connected with an output shaft of the rotating motor (43), the sleeve connection roller shaft (41) is provided with two groups, the sleeve connection roller shaft (41) is sleeved with the conveying belt (44), a mounting groove (441) is formed in the conveying belt (44), and a detection beaker (442) is arranged in the mounting groove (441).
7. The cloud database-based nitrilase enzyme property research alignment apparatus of claim 1, wherein: the outer cover (5) is fixed above the workbench (1), and the two ends of the outer cover (5) are provided with an inlet and outlet groove (51).
8. A method for performing research alignment of nitrilase enzyme property research alignment equipment based on cloud database according to claim 1, wherein the method comprises the following steps: the method comprises the following steps:
the method comprises the following steps: placing the detection beakers (442) filled with different nitrilase liquids on a mounting groove (441) on a conveyer belt (44), starting the conveyer belt (44) for transportation, and driving the detection beakers (442) to move to the lower part of the detection equipment (2);
step two: the telescopic device (22) drives the detection probe (23) to move downwards, the detection probe (23) is pressed into the detection beaker (442) to carry out quality detection on different nitrilase liquids, and the detection probe (23) transmits a detected structure into the detector (24);
step three: the detector (24) transmits the detected information to the comparison device (3), the collection unit (34) collects and combs the information, the quality inspection information is transmitted to the comparison unit (33), and a plurality of groups of data are compared through the cloud database (31);
step four: when the detection probe (23) is used for next detection, the telescopic device (22) drives the detection probe (23) to move upwards, the detection probe (23) retracts into the inner lantern ring (252), and the disinfection layer (253) and the cleaning layer (254) are used for disinfection and cleaning, so that the next detection is facilitated.
9. The method for research alignment of nitrilase enzyme property research alignment devices based on cloud database as claimed in claim 8 wherein: in the first step, an infrared sensor (211) is arranged below the detection box (21), and the infrared sensor (211) is used for detecting and positioning the beaker (442).
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| CN114965435A (en) * | 2022-06-15 | 2022-08-30 | 中验科技(福建)有限公司 | Enhanced Raman spectrometer for on-site drug monitoring and use method thereof |
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