CN112657564A

CN112657564A - Interface device and assembly thereof, culture chamber interface device and array interface device

Info

Publication number: CN112657564A
Application number: CN202011440281.4A
Authority: CN
Inventors: 邓玉林; 徐建栋; 任培
Original assignee: Beijing Ligong Genshu Technology Co ltd
Current assignee: Beijing Ligong Genshu Technology Co ltd
Priority date: 2020-12-07
Filing date: 2020-12-07
Publication date: 2021-04-16

Abstract

The invention provides a microfluidic chip interface device which comprises a pin penetrating device and a pin interface device, wherein the pin penetrating device comprises a sealing element, a fastening device and a pin, the pin penetrates through a through hole of the sealing element and the fastening device and is fixed at the inner part of a first chip, and the pin tail of the pin is communicated with a first chip channel extending to the outer part of the first chip. The pin interface device comprises a pin pipe lower part, a closing piece and a pin pipe upper part, wherein one side of the pin pipe upper part abuts against the closing piece, and the other side of the pin pipe upper part is communicated with a second chip channel extending to the outside of a second chip; one side of the lower part of the needle inserting tube is abutted against the closed piece, and the other side of the lower part of the needle inserting tube is communicated with the open groove at the bottom of the second chip; when the fastening device enters the open recess, the pin pierces the closure into the upper portion of the pin tube. The invention adopts a needling sealing scheme to solve the problems of complex and sealed interfaces and the like in the prior art, and ensures clean, closed, rapid and repeatable loading among chips.

Description

Interface device and assembly thereof, culture chamber interface device and array interface device

Technical Field

The invention relates to a microfluidic chip interface device and a corresponding interface device assembly, a culture chamber interface device and an array interface device thereof, belonging to the technical field of preparation of biological reaction or detection chips.

Background

Micro-fluidic chip

The microfluidic chip can realize the whole biological or chemical reaction process on a very small chip, and show the final result. This process requires an efficient coordination of the chip and external fluids with the control part, and fluidic interfacing of the chip is one of the critical technologies. The good sealing performance, the convenient mounting and dismounting mode and the socket technology which can be repeatedly used for many times can bring better application expansion to the microfluidic chip. The current common socket approaches include the following:

a clamping piece type: the fluid pipeline between the chips mainly adopts a compressed connection mode, namely, the loading between the chips is realized through the tight pressing of the clamp and the chips, for example, the chip interface structure disclosed in the attached figure 1 of the Chinese patent CN211303111U adopts a chip clamping mechanism to fix the joint on the lifting plate and controls the joint to move up and down through a knob, and meanwhile, the freedom degree of left and right movement is increased, and the position of the joint on one dimension is convenient to adjust. However, the aforementioned clip type interface has the problems of complicated structure and inconvenient disassembly of the device, and especially, in part of the prior art, the lead-out pipeline is often adhered by special colloid, so that the problems that the joint cannot be replaced or moved when fixed, the joint cannot be disassembled, and the service life is limited may also exist.

Screw locking formula: fluid pipelines between the chips are mainly connected by screws and nuts so as to complete the assembly and disassembly of the chips, for example, the chip interface structure disclosed in fig. 1 of chinese patent CN104226386A adopts devices such as fixing screws, chip and interface sealing members, sealing interface screwing nuts, sealing interface screwing screws, chip channel compression screws and the like to complete the connection between the microfluidic chips. However, the screw locking type interface has the problems of trivial parts and complex disassembly; secondly, the screw and the nut can contact the reaction sample liquid at the interface part, so that the problems of liquid leakage, overflow and other pollution are caused; thirdly, after the screw hole sites are fixed, the chip must adopt a specific pressing plate corresponding to the screw hole sites, and unless the pressing plate corresponding to the complex structure is manufactured, the chip is difficult to be applied to the chip with the complex structure, and the application range is narrow.

The bracket type: different chips are fixedly connected by adopting a support structure, and then the pipeline interface is closed by applying pressure, so that the chips and the flow path are hermetically connected. This configuration also requires relatively complicated external fixtures such as brackets, and thus has limitations in applicability.

Based on the foregoing background, the existing microfluidic chip interface technology has at least the following technical problems:

1. the external device of the fluid interface of the chip is complex, and the mechanical structure is complicated and difficult;

2. the chip and the fluid interface are separated to easily generate liquid leakage, overflow and other pollution risks;

3. the sealing of the chip fluid interface during plugging and unplugging can not be ensured; or

4. During the process of connecting the chip and the chip, particle contaminants may be introduced, such as dust, or debris on the chip may fall into the chip, thereby causing the particles to easily block the internal channels of the chip.

The invention provides a chip interface device for micro needle punching connection without a complex external fixing structure. In addition, the invention also provides an interface device assembly, a culture chamber interface device and an array interface device corresponding to the interface device, which can meet the biochemical reaction requirements under different environments.

Disclosure of Invention

The interface device comprises a pin puncturing device and a pin interface device, wherein the pin puncturing device is positioned in a first chip, a sealing element, a fastening device and a pin are sequentially arranged on the pin puncturing device from bottom to top in the vertical direction, a through hole is formed in the fastening device, a pin point of the pin can penetrate through the sealing element and the through hole in the fastening device from bottom to top, a pin tail of the pin is fixed in the first chip and is communicated with a first chip channel in the first chip, and the first chip channel extends to the outside of the first chip; the pin interface device is positioned in the second chip and is sequentially provided with a pin tube lower part, a closing piece and a pin tube upper part from bottom to top in the vertical direction, the pin tube upper part is close to the closing piece and is communicated with a second chip channel in the second chip on the side away from the closing piece, and the second chip channel extends to the outside of the second chip; the lower part of the needle inserting tube abuts against the closing part and is communicated with the opening groove at the bottom of the second chip at the side away from the closing part, so that the needle inserting tube is fixed at the bottom of the second chip; when the first chip and the second chip are combined in a loading mode, the fastening device of the first chip enters the opening groove of the second chip, the contact pin enters the opening groove of the second chip and the lower portion of the contact pin tube firstly and secondly, and the sealing piece is pierced to enter the upper portion of the contact pin tube.

Preferably, a filter element is inserted into the upper part of the pin tube on the side facing away from the closing element, and the filter element can communicate the upper part of the pin tube with the second chip channel. In the present invention, the absence of the filter element does not affect the fluid passage and the liquid transportation between the chips, but the filter element can be added to filter the sample liquid in order to better filter the sample liquid and ensure that the pipeline is not blocked.

Preferably, the material of the filter element can be selected from one or more of polyethylene, polypropylene, polyvinyl chloride, nylon, glass fiber or quartz sand. It should be noted that the selection of the filter element material needs to be determined according to the biochemical reaction or culture conditions, for example, for the acid reaction or culture conditions, the filter element material should be selected to be acid-resistant so as to avoid the influence of impurities and reduce the service life of the interface.

Preferably, the size of the fastening means is smaller than or equal to the size of the open recess, in order to place the fastening means in the open recess for better bonding of the chip. Further preferably, the fastening means may have a size equal to the size of the open recess to enhance the bonding.

Preferably, the fastening device comprises a fastening device screwing device arranged outside the first chip and a fastening device screwing interface arranged inside the first chip, the fastening device screwing device and the fastening device screwing interface are both provided with threads for screwing, and the fastening device screwing device and the fastening device screwing interface can be screwed mutually to realize loading and unloading. The purpose is that the fastening device screwing device on the first chip and the fastening device screwing interface are in split design, can be conveniently assembled and disassembled, and then old contact pins can be conveniently replaced and used. It should be noted that it is straightforward to design the fastening device as a single piece and still fall within the scope of the present invention.

Preferably, the material of the closing element can be one or more selected from polyisoprene rubber, butyl rubber, halogenated base rubber, silicon rubber, ethylene propylene diene monomer rubber, brominated isobutylene-p-methylstyrene copolymer or modified chlorosulfonated polyethylene. It should be noted that the selection of the material of the closure needs to be determined according to the biochemical reaction or culture conditions, for example, for the acid reaction or culture conditions, the material of the closure should be selected to be acid-resistant so as to avoid the influence of impurities and reduce the service life of the interface.

In addition, the invention also relates to an interface device assembly corresponding to the interface device, wherein the interface device assembly comprises a plurality of interface devices which are arranged in parallel, and the upper parts of the pin tubes of the plurality of pin interface devices are mutually communicated in a sealing way through a plurality of second chip channels. The method aims to complete the interaction of sample liquid through a plurality of second chip channels which are mutually and hermetically communicated, and comprises but not limited to (1) realizing sample injection, waste liquid collection and reciprocating sucking and beating in a culture or reaction environment, or (2) realizing sample injection of a plurality of samples and reagents and collection of a target object and waste liquid.

Optionally, the plurality of second chip channels of the interface device assembly may be connected in series or in parallel. The specific choice can be determined by the need of the reaction, such as the purpose of sample injection, waste liquid collection and reciprocating suction in the culture or reaction environment, the series connection mode can be adopted, and the parallel connection mode can be adopted for the purpose of sample injection of a plurality of samples and reagents, and the collection of the target object and the waste liquid.

The invention also relates to an interface device of the culture chamber. In particular, one or more culture chambers may be provided between a plurality of second chip channels of any of the aforementioned interface device assemblies. Which can facilitate the supply and replacement of the culture in the culture chamber.

The invention also relates to an array interface device. In particular, the array interface device comprises any one of the interface device assemblies described above, which are disposed in parallel and in which the plurality of first chip channels are in communication with each other. The use of an array of multiple interface device components as described above may help enable simultaneous operation of multiple chips.

Optionally, the plurality of first chip channels in the array interface device may be hermetically communicated in series or in parallel. The specific choice may depend on the reaction requirements.

The interface device has various technical effects, including but not limited to simple structure, convenient sample introduction and repeated use, and can realize the quick connection between a waste liquid flow path and a chip and the quick connection between multiple chips; the sealing performance is good, liquid leakage can be avoided, and the accidental blockage of the internal channel of the chip by particles in the plugging process can be reduced. In addition, the interface device assembly, the culture chamber interface device and the array interface device can meet the biochemical reaction requirements under different environments, and have wide applicability.

Drawings

FIG. 1A is a schematic plan view of the interface device 100 of the present invention in a separated state; FIG. 1B is a schematic view of the interface device 100 in an opened state; fig. 1C is a schematic plan view of the interface device 100 according to the present invention in a closed state; fig. 1D is a schematic overall configuration diagram of the closed state of the interface device 100 according to the present invention (fig. 1D).

Interface device 100, pin puncturing device 110, pin interface device 120, first chip 130, seal 111, fastening device 112, pins 113 (tips 113A, tails 113B), first chip 130, first chip channel 131, pin interface device 120, second chip 140, pin tube lower portion 121, closure 122, pin tube upper portion 123, second chip channel 141, open recess 142 are shown.

FIG. 2A is a schematic plan view of a preferred interface device 200 according to the present invention in a detached position; fig. 2B is an overall configuration diagram of the closed state of the preferred interface device 200 of the present invention.

Interface 200, fastening device 212 (fastening device screw-on device 212A and fastening device screw-on interface 212B), spike tube lower part 221, spike tube upper part 223, filter cartridge 224.

Fig. 3 is a schematic overall configuration diagram of a closed state of an interface device assembly 300 including two interface devices according to the present invention. Wherein the second chip channels are communicated in a serial manner.

The figure shows a chip interface device assembly 300, an interface device 310, an interface device 320, a second chip channel 331, and a second chip channel 332.

Fig. 4 is a schematic overall configuration diagram of a closed state of an interface device assembly 400 including three interface devices according to the present invention. And the second chip channels are communicated in a parallel connection mode.

The figure shows an interface device assembly 400, an interface device 410, an interface device 420, an interface device 430, a second chip channel 441 in communication with the interface device 410, a second chip channel 442 in communication with the interface device 420, a second chip channel 443 in communication with the interface device 430, and a main chip channel 444.

FIG. 5 is a schematic plan view of the culture chamber interface 500 of the present invention in a closed state.

In the figure, the culture chamber interface 500, the interface 510, the interface 520, the second chip channel 531 connected to the interface 510, the second chip channel 532 connected to the interface 520, and the culture chamber 540 are shown. Fig. 6 is an overall configuration diagram of the array interface device 600 according to the present invention in a closed state.

Array interface apparatus 600, interface apparatus assembly 610, interface apparatus assembly 620, interface apparatus assembly 630, interface apparatus assembly 640, interface apparatus assembly 650, first chip path 661, first chip path 662, first chip path 663.

Detailed Description

The following explains specific embodiments of the present invention. It is to be understood that the following examples are merely illustrative of particular embodiments, uses, and so forth of the invention in order to facilitate a better understanding of the relevant art and are not intended to limit the scope of the invention in any way. Various changes or modifications can be made to the invention by those skilled in the art, and equivalents may fall within the scope of the invention defined by the claims appended hereto

Example 1: interface device 100

As shown in fig. 1, the present invention provides an interface device 100, the interface device 100 includes a pin penetrating device 110 and a pin interface device 120, the pin penetrating device 110 is located inside a first chip 130, the pin penetrating device 110 is provided with a sealing member 111, a fastening device 112 and a pin 113 in sequence from bottom to top in a vertical direction, the fastening device 112 has a through hole inside, a pin tip 113A of the pin 113 can pass through the through holes inside the sealing member 111 and the fastening device 112 from bottom to top, a pin tail 113B of the pin is fixed inside the first chip 130 and is communicated with a first chip channel 131 inside the first chip 130, and the first chip channel 131 extends to the outside of the first chip 130; the pin interface device 120 is located inside the second chip 140, the pin interface device 120 is sequentially provided with a lower pin tube part 121, a sealing part 122 and an upper pin tube part 123 from bottom to top in the vertical direction, the upper pin tube part 123 abuts against the sealing part 122 and is communicated with a second chip channel 141 inside the second chip 140 on the side away from the sealing part 122, and the second chip channel 141 extends to the outside of the second chip 140; the lower part 121 of the cannula tube abuts against the closing element 122 and communicates with the open recess 142 of the bottom of the second chip 140 on the side facing away from the closing element 122, thus being fixed to the bottom of the second chip 140; when the first chip 130 and the second chip 140 are loaded and bonded, the fastening device 112 in the first chip 130 enters the open recess 142 of the second chip 140, and the pins 113 enter the open recess 142 of the second chip 140, the lower portion 121 of the pin tube first and second, piercing the closure 122 into the upper portion 123 of the pin tube.

Example 2: preferred interface device 200

As shown in fig. 2, the present invention provides a preferred interface device 200, which has the same structural features as those of embodiment 1, except that it further includes a filter element 224 on the basis of embodiment 1; the fastening device 212 is designed separately and comprises a fastening device screwing device 212A and a fastening device screwing interface 212B with threads for mutual screwing.

Specifically, the filter element 224 is disposed in the upper portion of the pin tube facing away from the closure and is in communication with the second chip channel in the interior of the second chip. The fastening device 212 comprises a fastening device screwing device 212A arranged outside the first chip and a fastening device screwing interface 212B arranged inside the first chip, the fastening device screwing device 212A and the fastening device screwing interface 212B are both provided with threads for screwing, and the fastening device screwing device 212A and the fastening device screwing interface 212B can be screwed mutually to realize assembling and disassembling.

Example 3: interface device assembly 300

As shown in FIG. 3, the present invention provides a preferred interface assembly 300 comprising two interfaces. The structural features of the interface device are the same as those of the previous embodiments 1-2, except that the interface device assembly 300 comprises the interface device 310 and the interface device 320 which are arranged in parallel in the vertical direction, wherein the second chip channel 331 communicated with the interface device 310 is directly communicated with the second chip channel 332 communicated with the interface device 320 in series, so that the purposes of sample injection, waste liquid collection and reciprocating sucking and injecting in a culture or reaction environment can be realized.

Example 4: interface device assembly 400

As shown in fig. 4, the present invention provides a preferred interface assembly 400 having three interfaces. The structural features of the interface device are the same as those of the previous embodiments 1-2, except that the interface device assembly 400 comprises an interface device 410, an interface device 420 and an interface device 430 which are disposed in parallel in the vertical direction, wherein the second chip channel 441 connected to the interface device 410, the second chip channel 442 connected to the interface device 420 and the second chip channel 443 connected to the interface device 430 are connected in parallel, so that the purpose of sampling, target and waste liquid collection for a plurality of samples and reagents can be achieved.

It should be noted that a person skilled in the art can also use simple variants to further implement the interface device assembly design of further interface devices.

Example 5: culture chamber interface 500

As shown in FIG. 5, the present invention provides an incubation chamber interface 500. Specifically, the structural features of the interface means inside the culture chamber interface means 500 are the same as those of examples 1-2, except that the culture chamber interface means 500 comprises an interface means 510, an interface means 520 placed in parallel in the vertical direction, wherein the second chip channel 531 communicating with the interface means 510 and the second chip channel 532 communicating with the interface means 520 are in series communication. The culture chamber interface device 500 can be directly inserted into the corresponding culture chamber 540 for nutrient supply, and after the use is finished, a new interface device component can be replaced, and the culture chamber interface device can be used after being continuously inserted.

It should be noted that a person skilled in the art can also use simple variants to further implement the design of the interface device assembly comprising more culture chambers or to implement the design of the second chip channels communicating in series.

Example 6: array interface device 600

As shown in fig. 6, the present invention provides an array interface apparatus 600. Specifically, the structural features of the interface module device inside the array interface device 600 are the same as those of the previous embodiments 1-2, except that the array interface device 600 comprises an interface device module 610, an interface device module 620, an interface device module 630, an interface device module 640, and an interface device module 650, which are disposed in parallel in the vertical direction, and the three first chip channels, which are in communication with the interface device module 610, the interface device module 620, the interface device module 630, the interface device module 640, and the interface device module 650, are further communicated, thereby achieving the multi-reaction environment requirement.

It should be noted that one skilled in the art can also adopt simple modifications to further implement the design of the array interface device including more interface component devices; or the first chip channels communicated with the interface device assemblies in the embodiment 6 are communicated in other serial or parallel modes to adapt to more reaction environment requirements.

Claims

1. A microfluidic chip interface device, comprising:

the interface device includes a pin piercing device and a pin interface device,

the contact pin penetrating device is positioned in a first chip, a sealing element, a fastening device and a contact pin are sequentially arranged on the contact pin penetrating device from bottom to top in the vertical direction, a through hole is formed in the fastening device, the needle point of the contact pin can penetrate through the through hole in the sealing element and the through hole in the fastening device from bottom to top, the needle tail of the contact pin is fixed in the first chip and is communicated with a first chip channel in the first chip, and the first chip channel extends to the outside of the first chip;

the pin interface device is positioned in the second chip and is sequentially provided with a pin tube lower part, a closing piece and a pin tube upper part from bottom to top in the vertical direction, the pin tube upper part is close to the closing piece and is communicated with a second chip channel in the second chip on the side away from the closing piece, and the second chip channel extends to the outside of the second chip; the lower part of the needle inserting tube abuts against the closing part and is communicated with the opening groove at the bottom of the second chip at the side away from the closing part, so that the needle inserting tube is fixed at the bottom of the second chip; when the first chip and the second chip are combined in a loading mode, the fastening device of the first chip enters the opening groove of the second chip, the contact pin enters the opening groove of the second chip and the lower portion of the contact pin tube firstly and secondly, and the sealing piece is pierced to enter the upper portion of the contact pin tube.

2. The microfluidic chip interface device of claim 1, wherein: and a filter element is inserted in one side of the upper part of the needle inserting tube, which is far away from the sealing part, and the filter element can be communicated with the upper part of the needle inserting tube and the second chip channel.

3. The microfluidic chip interface device of claim 2, wherein: the material of the filter element can be selected from one or more of polyethylene, polypropylene, polyvinyl chloride, nylon, glass fiber or quartz sand.

4. The microfluidic chip interface device of claim 1, wherein: the size of the fastening means is smaller than or equal to the size of the open recess.

5. The microfluidic chip interface device of claim 1, wherein: the fastening device comprises a fastening device screwing device arranged outside the first chip and a fastening device screwing interface arranged inside the first chip, wherein the fastening device screwing device and the fastening device screwing interface are both provided with threads for screwing, and the fastening device screwing device and the fastening device screwing interface can be screwed mutually.

6. The microfluidic chip interface device of claim 1, wherein: the material of the sealing element can be one or more selected from polyisoprene rubber, butyl rubber, halogenated base rubber, silicon rubber, ethylene propylene diene monomer rubber, brominated isobutylene-p-methylstyrene copolymer or modified chlorosulfonated polyethylene.

7. An interface device assembly, comprising a plurality of microfluidic chip interface devices according to any one of claims 1 to 6 arranged in parallel, wherein the upper portions of the pin tubes of the plurality of pin interface devices are in sealed communication with each other through a plurality of second chip channels.

8. The interface device assembly of claim 7, wherein the plurality of second chip channels are in closed communication in series or in parallel.

9. An interface device for a culture chamber, the interface device comprising the interface device assembly of any one of claims 7-8, and one or more culture chambers between the plurality of second chip channels.

10. An array interface device, comprising an interface device assembly according to any of claims 7-8, said interface device assembly being disposed in parallel and wherein a plurality of first chip channels are in communication with each other.

11. The array interface device of claim 10, wherein the first chip channels are in series or parallel sealed communication.