CN112656904A - Traditional Chinese medicine malt germination processing method - Google Patents

Abstract

The invention discloses a traditional Chinese medicine malt germination processing method, which comprises the following steps: s1, elutriating raw barley to remove impurities; s2, adding sufficient water into the raw barley obtained in the step S1, and soaking for 5 hours; s3, fishing out the soaked barley malt, placing the barley malt in a drainage container, and carrying out water spraying every day under the conditions that the temperature is 25 ℃ and the humidity is 70%, and keeping the barley malt moist, wherein the mass ratio of the water spraying amount to the barley malt is 1:1 every day; stopping germination when barley sprouts to 0.5 cm; s4, drying the germinated barley for 12 hours at 70 ℃ under normal pressure, wherein the water content of the barley malt after drying is 2.07%. The method solves the problem that the existing barley germination process cannot provide specific parameters, provides a better implementation method for germination and processing of the traditional Chinese medicine malt, has stable and feasible process, ensures the medicinal properties of the malt, and has very good practicability.

Description

Traditional Chinese medicine malt germination processing method

Technical Field

The invention relates to the technical field of traditional Chinese medicine processing, in particular to a germination processing method of traditional Chinese medicine malt.

Background

The traditional Chinese medicine malt has the main functions of promoting qi circulation and helping digestion, and is used for treating indigestion, abdominal distension and pain, spleen deficiency and poor appetite and the like; secondly, it is commonly used for treating women's lactation failure, breast distending pain, breast milk stasis, etc. The Chinese pharmacopoeia and the teaching material of the traditional Chinese medicine processing science are specified as follows: the malt is processed product of mature barley of Gramineae after germination and drying. Soaking wheat grains in water, keeping proper temperature and humidity, and drying in the sun or at low temperature when sprouts grow to about 5 mm. However, the prior art does not disclose specific parameters of the barley germination process, and further lacks quality detection indexes corresponding to the functional indications, so that the quality of the malt processing process cannot be scientifically judged.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide a germination processing method of traditional Chinese medicine malt.

The technical purpose of the invention is realized by the following technical scheme:

a germination processing method of traditional Chinese medicine malt comprises the following steps:

s1, elutriating raw barley to remove impurities;

s2, adding sufficient water into the raw barley obtained in the step S1, and soaking for 5 hours;

s3, fishing out the soaked barley malt, placing the barley malt in a drainage container, and carrying out water spraying every day under the conditions that the temperature is 25 ℃ and the humidity is 70%, and keeping the barley malt moist, wherein the mass ratio of the water spraying amount to the barley malt is 1:1 every day; stopping germination when barley sprouts to 0.5 cm;

s4, drying the germinated barley for 12 hours at 70 ℃ under normal pressure.

Further, in step S4, the moisture content of the barley malt after drying is 2.07%.

The invention has the following beneficial effects:

the method solves the problem that the existing barley germination process cannot provide specific parameters, provides a better implementation method for germination and processing of the traditional Chinese medicine malt, has stable and feasible process, ensures the medicinal properties of the malt, and has very good practicability.

Detailed Description

The present invention will be described in further detail with reference to examples.

Example 1

A germination processing method of traditional Chinese medicine malt comprises the following steps:

s1, elutriating raw barley to remove impurities;

s2, adding sufficient water into the raw barley obtained in the step S1, and soaking for 5 hours;

s3, fishing out the soaked barley malt, placing the barley malt in a drainage container, and carrying out water spraying every day under the conditions that the temperature is 25 ℃ and the humidity is 70%, and keeping the barley malt moist, wherein the mass ratio of the water spraying amount to the barley malt is 1:1 every day; stopping germination when barley sprouts to 0.5 cm;

s4, drying the germinated barley for 12 hours at 70 ℃ under normal pressure, wherein the water content of the barley malt after drying is 2.07%.

Performance detection

1. Preparation and measurement of sample solutions

Preparation of sample solution: germinating raw barley to a specified length (about 0.5 cm), drying, pulverizing, sieving, weighing coarse powder 10.00 g, placing in a conical flask with a plug, adding 80% methanol solution 50mL, ultrasonically extracting for 45min, extracting for 3 times, filtering, washing residue and container with appropriate amount of 80% methanol solution, mixing filtrates, placing in a water bath kettle at 60 deg.C, volatilizing, dissolving residue with 0.03% hydrochloric acid, and fixing volume in 10mL measuring flask to obtain sample solution.

2. Method for measuring total alkaloid content

Precisely measuring 1.0mL of sample solution, placing the sample solution in a separating funnel, adding 4.0mL of phosphate buffer solution with pH of 6.0, adding 2.0mL of bromothymol blue acid dye solvent, uniformly mixing, standing for 5min, adding 25mL of chloroform, fully shaking for 3min, standing for 1h, and separating chloroform solution. 1.0mL of 0.03% hydrochloric acid was added to obtain a blank solution, and the absorbance of the solution was measured at 415 nm.

3. Method for measuring content of barley malt alkali

The sample solution was filtered through a 0.45 μm aqueous microporous membrane to determine the content of hordenine as a sample solution. A barley malt alkali reference substance 3.75mg to 25mL measuring flask is precisely weighed, and 0.03% hydrochloric acid is added to form a reference substance solution with the concentration of 150 ug/mL. Phenomenex luna C8 column (250 mm. times.4.6 mm, 5 um); the mobile phase A is methanol, the mobile phase B is 0.05mol/L potassium dihydrogen phosphate (pH is adjusted to 7.1 by triethylamine), and isocratic elution is 5: 95; the flow rate is 1 mL/min; the detection wavelength is 226nm, and the sample injection amount is 20 uL; the column temperature was 25 ℃.

4. Amylase activity determination method

Preparing a coloring agent: weighing 1.0g of 3, 5-dinitrosalicylic acid (DNS) and dissolving in 20mL of 2mol/L sodium hydroxide solution, adding 50mL of purified water, adding 30g of potassium sodium tartrate and dissolving, diluting with purified water to 100mL, and sealing and storing.

Preparation of sample solution: precisely weighing 5.00g of coarse powder of a sample, adding 90mL of water and 10mL of sodium acetate-acetic acid buffer solution (pH5.6), soaking in water bath at 40 ℃ for 2h, filtering, taking 2mL to 10mL of filtrate in a measuring flask, and diluting to constant volume by using an extraction solvent to obtain a sample solution.

Determination of amylase activity: taking 0.1mL of sample solution into a corresponding 25mL measuring flask, heating the sample solution in water bath to 40 ℃, adding 1mL of 1% soluble starch solution which is preheated to 40 ℃, performing enzymolysis in water bath at the constant temperature of 40 ℃ for 15min, respectively adding 2.5mL of coloring agent, shaking up, placing in water bath, boiling for 5min, taking out, cooling, adding purified water to fix the volume to obtain the solution to be detected. Taking another 0.1mL of purified water, obtaining blank liquid by the same method, measuring the absorbance value at 540nm, and calculating the amylase activity. The amylase activity was expressed as the amount (g) of glucose obtained by hydrolyzing starch with 1g of a malt sample. The results are shown in Table 1.

TABLE 1

	Water content%	The germination percentage%	Length of bud cm	Total alkaloid content ug/g	The content of the barley malt alkaloid is ug/g	Amylase activity g/g
							Sample 1 to be tested	27.11	93	0.519	86.2953	63.4774	3.0036
Sample 2 to be tested	26.87	95	0.512	89.2799	61.1122	2.9727
							Sample to be tested 3	27.37	92	0.496	86.5305	58.6925	2.9172

The embodiments of the present invention are preferred embodiments of the present invention, and the scope of the present invention is not limited by these embodiments, so: all equivalent changes made according to the structure, shape and principle of the invention are covered by the protection scope of the invention.

Claims (2)

1. A germination processing method of traditional Chinese medicine malt is characterized in that: the method comprises the following steps:

s1, elutriating raw barley to remove impurities;

s2, adding sufficient water into the raw barley obtained in the step S1, and soaking for 5 hours;

s3, fishing out the soaked barley malt, placing the barley malt in a drainage container, and carrying out water spraying every day under the conditions that the temperature is 25 ℃ and the humidity is 70%, and keeping the barley malt moist, wherein the mass ratio of the water spraying amount to the barley malt is 1:1 every day; stopping germination when barley sprouts to 0.5 cm;

s4, drying the germinated barley for 12 hours at 70 ℃ under normal pressure.

2. The germination processing method of traditional Chinese medicine malt according to claim 1, characterized in that: in step S4, the moisture content of the barley malt after drying is 2.07%.