CN112646714A

CN112646714A - Cell culture dish bacterial infection experimental apparatus

Info

Publication number: CN112646714A
Application number: CN202011574948.XA
Authority: CN
Inventors: 付素芳
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-12-28
Filing date: 2020-12-28
Publication date: 2021-04-13

Abstract

The invention relates to the technical field of cell culture, and discloses a cell culture dish bacterial infection experimental device which comprises a frame, wherein fixing plates which are uniformly distributed are fixedly arranged in the frame, and supporting plates are fixedly arranged at the upper end and the lower end in the fixing plates. This cell culture dish bacterial infection experimental apparatus, upwards promote through the sliding plate, drive first gear and second gear rotation, first gear drives its internal connection gear rotation, it slides to connect the pinion rack that the gear can drive both ends about it at the rotation in-process, the effect of deuterogamying the stripper rod drives the stripper wheel and extrudees on the arc bag surface, the stripper wheel is when removing to arc bag convex part, arc utricule volume diminishes, be in the gassing state, the stripper wheel is when the concave part of arc bag slides, the arc bag is in the gassing state, can be with gas injection to the stationary ring this moment, in the retransmission culture tank, thereby the effect of automatic oxygen that provides has been realized.

Description

Cell culture dish bacterial infection experimental apparatus

Technical Field

The invention relates to the technical field of cell culture, in particular to a cell culture dish bacterial infection experimental device.

Background

Cell culture refers to a method for simulating in vivo environment in vitro to enable the in vivo environment to survive, grow, reproduce and maintain main structures and functions, and is usually completed in a cell culture dish, the required temperature is provided in an incubator, and in the culture process, a plurality of groups of cells need to be cultured simultaneously, so that on one hand, the accuracy of data can be increased, and on the other hand, errors can be reduced.

In the current cell culture process, the manual operation is completely relied on, the workload is large, and when one work is carried out each time, the cells are required to be recorded, wherein if any culture dish has problems in operation, the culture dish is required to be discarded, so the cell culture requirement is strict; wherein not only need provide a certain amount of oxygen in to the culture dish in the cell culture process, also need carry out the liquid feeding simultaneously in to the culture dish, the content of oxygen needs to be moderate, and too much or too little can lead to cell death, needs liquid evenly distributed in the culture dish during the liquid feeding.

In order to solve the problems, a cell culture dish bacterial infection experimental device is urgently needed.

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides a cell culture dish bacterial infection experimental device which has the advantages of simultaneously controlling a plurality of groups of culture dishes to finish the same operation, reducing the burden of workers and the like, and solves the problems of large workload and low fault tolerance rate of culture personnel in the cell culture process in the prior art.

(II) technical scheme

In order to achieve the purposes of simultaneously controlling a plurality of groups of culture dishes to finish the same operation, reducing the burden of workers and the like, the invention provides the following technical scheme: a cell culture dish bacterial infection experimental device comprises a frame, wherein fixed plates which are uniformly distributed are fixedly arranged in the frame, supporting plates are fixedly arranged at the upper end and the lower end of the inner part of each fixed plate, first gears are rotatably connected in the supporting plates, toothed plates are movably connected at the upper end and the lower end of each first gear, extrusion rods are fixedly arranged on the opposite surfaces of the toothed plates, extrusion wheels are movably arranged in the extrusion rods through connecting springs, arc-shaped bags are fixedly arranged at the upper end and the lower end of the inner part of each fixed plate, a fixing ring is fixedly arranged in the fixed plate and between the two arc-shaped bags, a splitter ring is fixedly arranged in the fixing ring, air inlet pipes which are uniformly distributed are fixedly arranged in the splitter ring, a culture tank is arranged in the fixing ring, and a push rod is fixedly arranged at the bottom end of the inner part of the frame, the rear end of the fixed ring is rotatably connected with a second gear, and the upper end of the push rod is fixedly provided with a sliding plate corresponding to the fixed ring.

Preferably, fixed mounting has the montant rather than vertical distribution in the backup pad, fixed mounting has reset spring between the upper and lower both ends of montant and the arc bag, upwards promote through the sliding plate, drive first gear and second gear rotation, first gear drives its internal connection gear and rotates, it can drive the pinion rack at both ends about it and slide to connect the gear at the rotation in-process, the effect of deuterogamying the stripper bar drives the extrusion wheel and extrudees on the arc bag surface, the extrusion wheel is when removing to arc bag bulge, the arc utricule is long-pending to diminish, be in the gassing state, when the extrusion wheel slides to the concave part of arc bag, the arc bag is in the gassing state, can spray the gas in the stationary ring this moment, in the culture tank of retransmission, thereby the automatic effect that provides oxygen has been realized.

Preferably, the first gear and the second gear are in meshed connection with the sliding plate, a connecting gear is fixedly mounted inside the first gear, the connecting gear is in meshed connection with the toothed plate, and the connecting gear rotates along with the rotation of the first gear.

Preferably, the connection relationship between the extrusion wheel and the arc-shaped bag is close contact, the arc-shaped bag is designed in a sealing manner, and is filled with sterilized oxygen, the oxygen is transmitted from the arc-shaped bag, then the second gear is driven to rotate under the action of the sliding plate, the second gear drives the shunt ring to rotate, then the air inlet pipe is continuously positioned at the air inlet of the arc-shaped bag, the oxygen from the arc-shaped bag enters the shunt ring, and then enters through the distribution of the shunt ring, so that the effect of providing a proper amount of oxygen is achieved.

Preferably, the fixing ring and the arc-shaped bag are communicated, the fixing ring is movably connected with the second gear, the second gear is driven to rotate by the sliding plate in the forward pushing process, and the second gear drives the shunt ring to rotate so as to drive the culture tank to rotate in the rotating process, so that liquid can be uniformly distributed in the culture tank in the liquid adding process, and the uniform mixing effect is achieved.

Preferably, the connection relationship between the shunt ring and the second gear is a fixed connection.

Preferably, the air inlet pipe is communicated with the culture tank.

Preferably, the lower end of the push rod is fixedly provided with a driving device, and the driving device is fixedly connected with the frame.

(III) advantageous effects

Compared with the prior art, the invention provides a cell culture dish bacterial infection experimental device, which has the following beneficial effects:

1. this cell culture dish bacterial infection experimental apparatus, upwards promote through the sliding plate, drive first gear and second gear rotation, first gear drives its internal connection gear rotation, it slides to connect the pinion rack that the gear can drive both ends about it at the rotation in-process, the effect of deuterogamying the stripper rod drives the stripper wheel and extrudees on the arc bag surface, the stripper wheel is when removing to arc bag convex part, arc utricule volume diminishes, be in the gassing state, the stripper wheel is when the concave part of arc bag slides, the arc bag is in the gassing state, can be with gas injection to the stationary ring this moment, in the retransmission culture tank, thereby the effect of automatic oxygen that provides has been realized.

2. This cell culture dish bacterial infection experimental apparatus comes oxygen through the transmission in the arc bag, then under the effect of sliding plate, drives the second gear and rotates, and the second gear drives the splitter ring and rotates, then continuous messenger's intake pipe is located arc bag air inlet, and oxygen from the arc bag enters into the splitter ring, then the distribution through the splitter ring gets into to the effect that can provide suitable amount of oxygen has been reached.

3. This cell culture dish bacterial infection experimental apparatus promotes the in-process forward through the sliding plate, drives the second gear and rotates, and the second gear drives the reposition of redundant personnel ring and rotates the in-process and drive the culture tank and rotate, has guaranteed at the liquid feeding in-process, can make liquid evenly distributed in the culture tank, has reached the effect of homogeneous mixing.

Drawings

FIG. 1 is a schematic front view of the overall structure of the present invention;

FIG. 2 is a schematic view of a fixing plate according to the present invention;

FIG. 3 is a schematic view of the arcuate bladder of the present invention;

FIG. 4 is a schematic view of the connection of the slide plate to the first and second gear structures of the present invention;

FIG. 5 is an enlarged view of the structure A in FIG. 3 according to the present invention.

In the figure: 1. a frame; 2. a fixing plate; 3. a support plate; 4. a first gear; 5. a toothed plate; 6. an extrusion stem; 7. a connecting spring; 8. an extrusion wheel; 9. an arc-shaped bag; 10. a fixing ring; 11. a shunt ring; 12. an air inlet pipe; 13. a culture tank; 14. a push rod; 15. a second gear; 16. a sliding plate; 17. a vertical rod; 18. a return spring.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-5, a cell culture dish bacterial infection experimental apparatus comprises a frame 1, a fixed plate 2 is fixedly installed inside the frame 1, support plates 3 are fixedly installed at the upper and lower ends inside the fixed plate 2, a first gear 4 is rotatably connected inside the support plates 3, the first gear 4 and a second gear 15 are in meshed connection with a sliding plate 16, a connecting gear 19 is fixedly installed inside the first gear 4, the connecting gear 19 is in meshed connection with a toothed plate 5, the connecting gear 19 rotates along with the rotation of the first gear 4, a vertical rod 17 vertically distributed on the support plate 3 is fixedly installed on the support plate 3, a return spring 18 is fixedly installed between the upper and lower ends of the vertical rod 17 and an arc-shaped bag 9, the first gear 4 and the second gear 15 are driven to rotate by pushing the sliding plate 16 upwards, the first gear 4 drives the internal connecting gear 19 to rotate, connect gear 19 and can drive the pinion rack 5 at its upper and lower both ends and slide at the rotation in-process, the effect of deuterogamying the stripper bar 6 drives the pinch roller 8 and extrudees on the surface at arc bag 9, pinch roller 8 is when moving to arc bag 9 convex part, arc bag 9 volume diminishes, be in the gassing state, pinch roller 8 is when the concave part of arc bag 9 slides, arc bag 9 is in the gassing state, can spray gas to solid fixed ring 10 this moment, again in the transmission cultivates groove 13, thereby the effect of automatic oxygen that provides has been realized.

Equal swing joint in upper and lower both ends of first gear 4 has pinion rack 5, fixed mounting has squeeze bar 6 on the looks back of pinion rack 5, there is extrusion wheel 8 inside of squeeze bar 6 through connecting spring 7 movable mounting, equal fixed mounting in upper and lower both ends of fixed plate 2 inside has arc bag 9, the relationship of connection of extrusion wheel 8 and arc bag 9 is in close contact with, arc bag 9 is sealed design, and inside packing has through sterile oxygen, transmit oxygen through in the arc bag 9, then under the effect of sliding plate 16, drive second gear 15 and rotate, second gear 15 drives splitter ring 11 and rotates, then continuous messenger's intake pipe 12 is located arc bag 9 air inlet, oxygen from arc bag 9 enters into splitter ring 11, then the distribution through splitter ring 11 gets into, thereby reached the effect that can provide suitable amount of oxygen.

A fixed ring 10 is fixedly arranged in the fixed plate 2 and between the two arc-shaped bags 9, the fixed ring 10 and the arc-shaped bags 9 are in a communicating design, the connecting relationship between the fixed ring 10 and the second gear 15 is movably connected, the second gear 15 is driven to rotate in the forward pushing process through a sliding plate 16, the second gear 15 drives the diverter ring 11 to rotate so as to drive the culture tank 13 to rotate, the liquid can be uniformly distributed in the culture tank 13 in the liquid adding process, the uniform mixing effect is achieved, the diverter ring 11 is fixedly arranged in the fixed ring 10, the connecting relationship between the diverter ring 11 and the second gear 15 is fixedly connected, the uniformly distributed air inlet pipe 12 is fixedly arranged in the diverter ring 11, the air inlet pipe 12 is communicated with the culture tank 13, the culture tank 13 is arranged in the fixed ring 10, a push rod 14 is fixedly arranged at the bottom end in the frame 1, the lower end of the push rod 14 is fixedly provided with a driving device, the driving device is fixedly connected with the frame 1, the rear end of the fixed ring 10 is rotatably connected with a second gear 15, and the upper end of the push rod 14 is fixedly provided with a sliding plate 16 corresponding to the fixed ring 10.

The working principle is as follows: when the cell culture dish bacterial infection experimental device is used, the sliding plate 16 pushes upwards to drive the first gear 4 and the second gear 15 to rotate, the first gear 4 drives the internal connecting gear 19 to rotate, the connecting gear 19 can drive the toothed plates 5 at the upper end and the lower end of the connecting gear to slide in the rotating process, the extrusion rod 6 is matched to drive the extrusion wheel 8 to extrude on the surface of the arc-shaped bag 9, when the extrusion wheel 8 moves to the convex part of the arc-shaped bag 9, the arc-shaped bag 9 is reduced in size and in a deflation state, when the extrusion wheel 8 slides to the concave part of the arc-shaped bag 9, the arc-shaped bag 9 is in a deflation state, at the moment, gas can be sprayed into the fixing ring 10 and then is transmitted into the culture tank 13, and therefore the effect of automatically providing oxygen is achieved; oxygen is transmitted from the arc-shaped bag 9, then the second gear 15 is driven to rotate under the action of the sliding plate 16, the second gear 15 drives the diverter ring 11 to rotate, then the air inlet pipe 12 is continuously positioned at the air inlet of the arc-shaped bag 9, the oxygen from the arc-shaped bag 9 enters the diverter ring 11 and then enters through the distribution of the diverter ring 11, and therefore the effect of providing proper amount of oxygen is achieved; the sliding plate 16 drives the second gear 15 to rotate in the forward pushing process, and the second gear 15 drives the diverter ring 11 to rotate so as to drive the culture tank 13 to rotate, so that liquid can be uniformly distributed in the culture tank 13 in the liquid adding process, and the uniform mixing effect is achieved.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a cell culture dish bacterial infection experimental apparatus, includes frame (1), its characterized in that: the inner part of the frame (1) is fixedly provided with uniformly distributed fixed plates (2), the upper and lower ends of the inner part of the fixed plates (2) are fixedly provided with supporting plates (3), the inner part of the supporting plates (3) is rotatably connected with first gears (4), the upper and lower ends of the first gears (4) are movably connected with toothed plates (5), the back surfaces of the toothed plates (5) are fixedly provided with extrusion rods (6), the inner part of the extrusion rods (6) is movably provided with extrusion wheels (8) through connecting springs (7), the upper and lower ends of the inner part of the fixed plates (2) are fixedly provided with arc-shaped bags (9), the inner part of the fixed plates (2) is fixedly provided with a fixed ring (10) between the two arc-shaped bags (9), the inner part of the fixed ring (10) is fixedly provided with a shunting ring (11), and the inner part of the shunting ring (11) is fixedly provided with uniformly distributed air inlet, the culture tank (13) is arranged inside the fixing ring (10), the bottom end inside the frame (1) is fixedly provided with a push rod (14), the rear end of the fixing ring (10) is rotatably connected with a second gear (15), and the upper end of the push rod (14) is fixedly provided with a sliding plate (16) corresponding to the fixing ring (10).

2. The device for testing bacterial infection of a cell culture dish according to claim 1, wherein: the supporting plate (3) is fixedly provided with vertical rods (17) vertically distributed with the supporting plate, and reset springs (18) are fixedly arranged between the upper end and the lower end of each vertical rod (17) and the arc-shaped bags (9).

3. The device for testing bacterial infection of a cell culture dish according to claim 1, wherein: the connecting relationship between the first gear (4) and the sliding plate (16) and between the second gear (15) and the sliding plate is in meshed connection, a connecting gear (19) is fixedly installed inside the first gear (4), and the connecting gear (19) is in meshed connection with the toothed plate (5).

4. The device for testing bacterial infection of a cell culture dish according to claim 1, wherein: the extrusion wheel (8) is in close contact with the arc-shaped bag (9), the arc-shaped bag (9) is designed in a sealing mode, and the inside of the arc-shaped bag is filled with sterilized oxygen.

5. The device for testing bacterial infection of a cell culture dish according to claim 1, wherein: the fixing ring (10) and the arc-shaped bag (9) are communicated, and the fixing ring (10) is movably connected with the second gear (15).

6. The device for testing bacterial infection of a cell culture dish according to claim 1, wherein: the connection relation between the shunt ring (11) and the second gear (15) is fixed connection.

7. The device for testing bacterial infection of a cell culture dish according to claim 1, wherein: the air inlet pipe (12) is communicated with the culture tank (13).

8. The device for testing bacterial infection of a cell culture dish according to claim 1, wherein: the lower end of the push rod (14) is fixedly provided with a driving device, and the driving device is fixedly connected with the frame (1).