CN112645446A - Starting method of one-stage SBR-anammox denitrification system - Google Patents
Starting method of one-stage SBR-anammox denitrification system Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
- C02F3/307—Nitrification and denitrification treatment characterised by direct conversion of nitrite to molecular nitrogen, e.g. by using the Anammox process
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- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
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- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/006—Apparatus and plants for the biological treatment of water, waste water or sewage details of construction, e.g. specially adapted seals, modules, connections
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/02—Temperature
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/06—Controlling or monitoring parameters in water treatment pH
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/14—NH3-N
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/44—Time
Abstract
The invention discloses a one-stage SBR-anammox system starting method, which starts a one-stage partial nitrosation anaerobic ammonia oxidation system by starting short-range nitrosation and then starting anaerobic ammonia oxidation. By controlling the ammonia nitrogen concentration of the inlet water, HRT and DO respectively start shortcut nitrification and anaerobic ammonia oxidation by 75d and 58 d. The method comprises the following steps: (1) in the short-cut nitration stage, a certain proportion of filler is added into a reactor. (2) The return sludge of the secondary sedimentation tank is taken as inoculation sludge, the simulated sewage is taken as test water, DO, temperature, pH and the concentration of the inflow substrate are strictly controlled, and the short-cut nitrification is directly started after the aeration is successful; (3) then inoculating a small amount of anaerobic ammonia oxidation sludge in the reactor to quickly start anaerobic ammonia oxidation so as to start one-stage partial nitrosation anaerobic ammonia oxidation. The method has the effects that the superior bacteria of the system are balanced by the operation conditions through the addition of the filler and the change of the impact load to start a part of the nitrosation anaerobic ammonia oxidation system, so that the denitrification of the autotrophs is realized, and the operation cost is reduced.
Description
The technical field is as follows:
the invention belongs to the technical field of water treatment, and particularly relates to a one-stage SBR-anammox denitrification system starting method which is suitable for treating sewage containing ammonia nitrogen.
Background art:
in recent years, the one-stage partial nitrosation anaerobic ammonia oxidation technology becomes a research hotspot in the field of sewage denitrification and dephosphorization due to the advantages of simple process, low treatment cost and the like. The principle is that AOB (aerobic ammonium-oxidizing bacteria) is utilized to oxidize partial ammonia nitrogen in sewage into NO in partial nitrosation stage2 --N is used as an anaerobic ammonia oxidation electron acceptor, and then ammonia nitrogen remained in the sewage is used as an electron donor and generated NO in the anaerobic ammonia oxidation stage2 -N generates nitrogen gas by the action of AnAOB (anaerobic ammonium-oxidizing bacteria). The technology of one-stage short-cut nitrification-anaerobic ammonia oxidation for denitrification from sewage is considered to be an effective, economical and low-energy-consumption method compared with the traditional nitrification and denitrification processes. To date, over one hundred production scale sewage plants have been operated worldwide using anammox technology to treat high ammonia nitrogen wastewater, and this figure is still increasing rapidly. Based on the anaerobic ammonia oxidation process, SBR and RBC reactors are adopted more frequently. However, since RBC wastewater treatment capacity is relatively small and biofilm easily falls off, SBR is considered to be the most suitable reactor for the growth of anammox bacteria, and is the main reactor application.
At present, one of the major bottlenecks in the application of the one-stage partial nitrosation anaerobic ammonia oxidation technology is the starting time, the starting of the reactor is the premise and the basis of the whole experimental process, the essence of the starting is to balance two types of functional bacteria in the reactor, namely Ammonia Oxidizing Bacteria (AOB) and anaerobic Ammonia Oxidizing Bacteria (AOB) and inhibit the growth of Nitrite Oxidizing Bacteria (NOB), complex competition relationships exist among the AOB, the AOB and the NOB, and how to cooperate the relationship between the AOB and the AnAOB in a short time is a challenge for the one-stage partial nitrification anaerobic ammonia oxidation technology. Therefore, it is necessary to investigate the shortening of the reactor start-up time.
The starting time of the one-stage type shortcut nitrification anaerobic ammonia oxidation is different due to different starting modes and operating conditions. By adding biofilm carriers such as filler and the like into the reactor, the impact load resistance and the environmental adaptability of the reactor are improved, functional bacteria are enriched, a good anaerobic environment is provided for AnAOB, the biomass is increased, the bacteria are not easy to lose, and the sludge settling property can be improved. Therefore, the one-section type partial nitrosation anaerobic ammonium oxidation denitrification system can be quickly started by adding the biofilm carrier, good operation conditions and adding a proper amount of anaerobic ammonium oxidation bacterial sludge.
Disclosure of Invention
The invention aims to solve the problems that a one-stage partial nitrosation anaerobic ammonia oxidation denitrification system is long in starting time and easy to lose functional bacteria.
In order to achieve the purpose, the technical scheme adopted by the invention is that the whole system is started by adding a biofilm carrier into SBR, controlling the operation condition, adding seed sludge, starting partial nitrosation, and then starting anaerobic ammonia oxidation. The starting time of partial nitrosation and anaerobic ammoxidation is 75d and 58d respectively. The method comprises the following specific steps:
(1) short-cut nitrification is started.
Inoculating sludge, and strictly controlling DO, temperature, pH, influent substrate concentration, aeration and other conditions.
According to the invention, the operation condition is preferably selected, DO is controlled to be between 0.2 and 0.3mg/L, the temperature is 30 +/-2 ℃, the pH is 7.5 to 8.2, the HRT is 24 hours, the concentration of the substrate of the fed water (NH of the initial fed water) is controlled4 +-N is 50mg/L) water-intake base consisting of NH4HCO3The organic carbon source was not added, and the feed water was not subjected to nitrogen blowing treatment. Aerobic sludge of a sewage treatment plant is inoculated by short-cut nitrification, and the sludge amount accounts for 60 percent of the effective volume (14L) of the reactor.
According to the invention, the operation condition is optimized, the short-cut nitrification stage is operated in an intermittent water inlet and outlet mode, three periods are operated every day, the period is 8h, wherein the water inlet time is 20min, the aeration/stirring time is 6h, the standing time is 80min, and the water drainage time is 20 min. The drainage ratio was 33.3%.
According to the invention, the short-cut nitrification stage is provided with the inlet water ammonia nitrogen concentration gradient to form biological selective pressure to gradually eliminate NOB, and the inlet water ammonia nitrogen concentration gradients are respectively 50mg/L, 70mg/L, 100mg/L and 120 mg/L.
In the water supply NH4 +And when the-N is controlled to be 100mg/L, filling materials are added into the reactor, and the filling ratio is 30%. The filler is High Density Polyethylene (HDPE) filler with diameter of 10 × 10mm and specific gravity of 0.95g/cm3Above, the specific surface area is more than 500m2/m3。
(2) Starting anaerobic ammonia oxidation.
Starting anaerobic ammonia oxidation after stabilization of partial nitrification stage, namely, effluent NH4 +-N is around 22mg/L, NH4 +The N removal rate is stabilized at 51 percent, NAR, namely the accumulation rate of nitrite nitrogen is stabilized at 52 percent, at the moment, a little anaerobic ammonia oxidation sludge accounting for 1/50 of the effective volume (14L) of the reactor is added into the reactor, the aeration time is shortened from 6h to 2h, and the period is changed into water feeding for 20min, aeration/stirring for 2h, stirring for 4h, standing for 80min and water draining for 20 min. At the initial stage of starting up the anammox, DO is still controlled to be 0.2mg/L, and HRT is 24 h.
After the reactor is operated for 104d, although the effluent quality fluctuates slightly, the total nitrogen removal performance of the reactor is obviously recovered, and the operation condition is good. At this time, the MLSS in the reactor was about 2650mg/L, and the SVI was 82 mL/g.
The invention has the advantages that (1) the filler is added to serve as a biological membrane to effectively prevent sludge loss, so that a microstructure is formed in the reactor, the impact load resistance and the environmental adaptability of the reactor are improved, functional bacteria are enriched, a good anaerobic environment is provided for AnAOB, the biomass is increased, the thallus is not easy to lose, and the sludge settling property can be improved. (2) The anaerobic ammonia oxidation is started after the partial nitrosation is started, the one-section type partial nitrosation anaerobic ammonia oxidation is quickly started by adopting intermittent aeration and controlling the running conditions of the ammonia nitrogen concentration of inlet water, DO and the like, the synergy between AOB and AAOB is effectively balanced and NOB is inhibited, the starting method is simple and easy to execute, and the denitrification effect is good.
Drawings
FIG. 1 is a diagram showing the apparatus of a one-stage SBR-anammox reactor according to an embodiment of the present invention.
Wherein: 1. a water inlet pump; 2. an electric stirrer; 3. a stirring paddle; 4. a heating rod; a water outlet; 6. a sampling port; 7. aeration plate
Detailed Description
The starting method of the one-stage SBR-anammox denitrification system is further explained by the concrete implementation example.
The examples are not described in detail as they are known in the art.
Examples
A one-stage SBR-anammox denitrification system starting method comprises the following steps:
(1) inoculating sludge
The experimental inoculation sludge is taken from return sludge of a secondary sedimentation tank of a certain Tianjin sewage treatment plant, MLSS is 4500mg/L, and the inoculation amount is about 60 percent of the effective volume (14L) of the reactor.
(2) Reactor and packing
The SBR reactor is adopted in the test, the effective volume of the main body of the SBR reactor is a cylindrical organic glass container of 14L, the height of the SBR reactor is 600mm, the diameter of the cylindrical SBR reactor is 200mm, and a row of sampling ports with the spacing of 100mm are arranged in the vertical direction on the wall of the SBR reactor and used for sampling and draining.
In the water supply NH4 +And when the-N is controlled to be 100mg/L, filling materials are added into the reactor, and the filling ratio is 30%. The filler is High Density Polyethylene (HDPE) filler with diameter of 10 × 10mm and specific gravity of 0.95g/cm3Above, the specific surface area is more than 500m2/m3。
(3) Culture medium components and content thereof
The test water adopts artificial simulated wastewater, and the water inlet substrate is composed of NH4HCO3And NaNO2Provided, no organic carbon source was added.
TABLE 1 Artificial simulation of wastewater quality
Table 2 shows the composition and content of trace elements
(4) Reactor operating parameters
Starting stage of shortcut nitrification (1 st-75 d):
basic parameters: controlling DO at 0.2-0.3mg/L, temperature at 30 + -2 deg.C, pH at 7.5-8.2, HRT at 24 hr, and water inlet substrate concentration (initial water inlet NH)4 +-N is 50 mg/L).
The operation period is as follows: the method is operated by adopting an intermittent water inlet and outlet mode, three periods are operated every day, one period is 8 hours, wherein the water is fed for 20 minutes, the aeration/stirring is carried out for 6 hours, the standing is carried out for 80 minutes, and the water is drained for 20 minutes. The drainage ratio was 33.3%.
Ammonia nitrogen concentration of raw water: the ammonia nitrogen concentration of the raw water inlet is 50mg/L on days 1-10, 70mg/L on days 11-14, 100mg/L on days 15-37, and 120mg/L on days 38-75.
During the start of short-cut nitrification, water NH is fed4 +And when the-N is controlled to be 100mg/L, filling materials are added into the reactor, and the filling ratio is 30%. The filler is High Density Polyethylene (HDPE), has a diameter of 10 × 10mm, specific gravity of 0.95g/cm3 or more, and specific surface area of more than 500m2/m3。
And (3) operating results: after the reactor is operated to the 54 th day, the reactor enters a stable operation stage (54d-78d), and water NH is discharged4 +-N is around 22mg/L, NH4 +The removal rate of-N is stabilized at about 51 percent, and NO is discharged2 -N rises slowly and NOR decreases to 3.2 g/(m)3D) and the AOR value is continuously increased, and the NAR (nitrite nitrogen accumulation rate) is stabilized at about 52 percent, which shows that the shortcut nitrification is successfully started.
② anaerobic ammonium oxidation start stage (83-140 d)
Starting anaerobic ammonia oxidation after stabilization of partial nitrification stage, namely, effluent NH4 +-N is around 22mg/L, NH4 +The N removal rate stabilized at 51% and the NAR, i.e.nitrite nitrogen accumulation rate stabilized at 52%, at which point a small amount of anammox sludge was added to the reactor, representing about 1/50 of the effective volume (14L) of the reactor.
Basic parameters: controlling DO at 0.2mg/L, temperature of 30 +/-2 ℃, pH of 7.5-8.2 and HRT of 24 h.
The operation period is as follows: the water-saving device runs in an intermittent water inlet and outlet mode and runs for three periods every day. Aeration is shortened from 6h to 2 h. The period is 20min for water inflow, 2h for aeration/stirring, 4h for stirring, 80min for standing and 20min for water drainage. The drainage ratio was 33.3%.
Ammonia nitrogen concentration of raw water: the ammonia nitrogen concentration of the inlet water from 83 th to 140 th day is 120mg/L according to the judgment of the water quality of the outlet water of the reactor.
And (3) operating results: after the reactor is operated for 104d, although the effluent quality fluctuates slightly, the total nitrogen removal performance of the reactor is obviously recovered, and the operation condition is good. At this time, the MLSS in the reactor was about 2650mg/L, and the SVI was 82 mL/g. TRE rises obviously and water NH is discharged4 +The N is reduced to 1.4mg/L from 26.7mg/L before the seed sludge is added, and basically NO NO can be detected in effluent2 -N, NO, which is indicative of the formation of part of the nitrosation stage2 -N, is almost completely consumed in the anaerobic phase. Subsequently, NAR is basically stabilized at about 68% in one month, and NOR is reduced to 1.45 g/(m)3D) AOR is on average 2.1 g/(m)3D) approximately, the NAR can reach 78./4% at the highest, and the TRE can reach 49.8% at the highest. Delta NO removed by reaction throughout the agitated anaerobic phase2 --N and Δ NH4 +Average N ratio of 1.38, resulting in Δ NO3 --N and removed Δ NH4 +The average value of-N ratio is 0.32, which is obviously slightly higher than the theoretical value, and the anaerobic ammonia oxidation is successfully started, namely the one-stage SBR-partial nitrosation anaerobic ammonia oxidation is successfully started. NH after operation stabilization4 +The maximum degradation amount of-N can reach 97.67 percent, and the maximum degradation amount of TREThe height can reach 56.86%.
Claims (8)
1. A starting method of a one-stage SBR-anammox denitrification system is characterized in that a partial nitrosation stage is started first, then an anaerobic ammonia oxidation stage is started, and further one-stage partial nitrosation anaerobic ammonia oxidation is started. By adding a certain proportion of filler into the SBR reactor, successfully and directly starting partial nitrosation by controlling the operating conditions such as pH, temperature, DO, aeration quantity and the like; then inoculating a small amount of anaerobic ammonium oxidation sludge into the reactor to quickly start anaerobic ammonium oxidation. The method comprises the following steps:
(1) inoculating a certain amount of return sludge of a secondary sedimentation tank of a sewage treatment plant, wherein the amount of the sludge accounts for 1/50 of the effective volume (14L) of the reactor. Strictly controlling DO (0.2-0.3mg/L), temperature (30 +/-2 ℃), pH (7.5-8.2) and water inlet substrate concentration (50-120mg/L), and directly starting short-cut nitrification by aeration;
(2) the filler (packing ratio 30%) was added as biofilm carrier during the start of the shortcut nitrification.
(3) A small amount of anammox sludge which accounts for about 1/50 of the effective volume (14L) of the reactor is added into the reactor when the shortcut nitrification is started successfully, and the anaerobic ammonia oxidation is started by controlling the aeration stirring time (shortened from 6h to 4h), the period (3 periods per day), DO (0.2-0.3mg/L) and HRT (24 h).
2. The method for starting the one-stage SBR-partial nitrosation anaerobic ammonium oxidation system according to claim 1, wherein: filling High Density Polyethylene (HDPE) filler phi 10 x 10mm with specific gravity of 0.95g/cm into the reactor at short distance nitration stage3Above, the specific surface area is more than 500m2/m3The filling ratio of the inoculation filler is about 30 percent.
3. The method for starting the one-stage SBR-partial nitrosation anaerobic ammonium oxidation system according to claim 1, wherein: controlling DO at 0.2-0.3mg/L, temperature at 30 + -2 deg.C, pH at 7.5-8.2, HRT at 24 hr, and water inflow substrate concentration (initial water inflow NH)4 +-N is 50mg/L) water-intake base consisting of NH4HCO3The organic carbon source was not added, and the feed water was not subjected to nitrogen blowing treatment.
4. The method for starting the one-stage SBR-partial nitrosation anaerobic ammonium oxidation system according to claim 1, wherein: aerobic sludge of a sewage treatment plant is inoculated by short-cut nitrification, the sludge amount is about 60 percent of the effective volume (14L) of the reactor, the MLSS of the inoculated sludge is 4500mg/L, and the SVI is 63 mL/g. After the short-cut nitrification phase was stabilized, the reactor had an MLSS of 2800mg/L and an SVI of 84 mL/g.
5. The method for starting the one-stage SBR-partial nitrosation anaerobic ammonium oxidation denitrification system of claim 1, wherein: in the short-cut nitrification starting stage, the ammonia nitrogen concentration of the raw water inlet is 50mg/L in 1 st to 10 th days, the ammonia nitrogen concentration of the raw water inlet is 70mg/L in 11 th to 14 th days, the ammonia nitrogen concentration of the raw water inlet is 100mg/L in 15 th to 37 th days, and the ammonia nitrogen concentration of the raw water inlet is 120mg/L in 38 th to 75 th days.
6. The method for starting the one-stage SBR-partial nitrosation anaerobic ammonium oxidation denitrification system of claim 1, wherein: the short-cut nitrification stage is operated by adopting an intermittent water inlet and outlet mode, three periods are operated every day, one period is 8 hours, wherein the water is fed for 20 minutes, the aeration/stirring is carried out for 6 hours, the standing is carried out for 80 minutes, and the water is drained for 20 minutes. The drainage ratio was 33.3%.
7. The method for starting the one-stage SBR-partial nitrosation anaerobic ammonium oxidation denitrification system of claim 1, wherein: short-cut nitrification stabilization phase (effluent NH)4 +-N is around 22mg/L, NH4 +The N removal rate is stabilized at 51 percent, NAR (nitrite nitrogen accumulation rate) is stabilized at 52 percent, a little anaerobic ammonia oxidation sludge accounting for 1/50 of the effective volume (14L) of the reactor is added into the reactor, the aeration time is shortened to 2 hours, and the period is changed into water feeding for 20 minutes, aeration/stirring for 2 hours, stirring for 4 hours, standing for 80 minutes and water draining for 20 minutes. At the beginning of starting up of anammox, DO was controlled at 0.2mg/L and HRT was controlled at 24 hours.
8. The method for starting the one-stage SBR-partial nitrosation anaerobic ammonium oxidation denitrification system of claim 1, wherein: anaerobic ammonia oxidation starting stage: the short-cut nitrification reaches a stable stage (water outlet NH)4 +-N is around 22mg/L, NH4 +After the-N removal rate is stabilized at 51% and the nitrite nitrogen accumulation rate is stabilized at 52%, anaerobic ammonia oxidation is started. The ammonia nitrogen concentration of the inlet water from 83 th to 140 th is 120mg/L, the ammonia nitrogen concentration of the inlet water from 141 th to 160 th is 140mg/L, the NAR is basically stabilized at about 68% in the later period, and the integral operation condition exceeds the earlier period. The NOR is reduced to 1.45 g/(m) at this stage3D) AOR is on average 2.1 g/(m)3D) about, the highest nitrite nitrogen accumulation rate can reach 78.4%, the highest TRE can reach 49.8%, the anaerobic ammonia oxidation is successfully started, and then partial nitrosation anaerobic ammonia oxidation is successfully started.
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Application publication date: 20210413 |