CN112641520B - Dual-mode operation microscope for skin cancer treatment - Google Patents
Dual-mode operation microscope for skin cancer treatment Download PDFInfo
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- CN112641520B CN112641520B CN202011536365.8A CN202011536365A CN112641520B CN 112641520 B CN112641520 B CN 112641520B CN 202011536365 A CN202011536365 A CN 202011536365A CN 112641520 B CN112641520 B CN 112641520B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B90/00—Instruments, implements or accessories specially adapted for surgery or diagnosis and not covered by any of the groups A61B1/00 - A61B50/00, e.g. for luxation treatment or for protecting wound edges
- A61B90/20—Surgical microscopes characterised by non-optical aspects
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B90/00—Instruments, implements or accessories specially adapted for surgery or diagnosis and not covered by any of the groups A61B1/00 - A61B50/00, e.g. for luxation treatment or for protecting wound edges
- A61B90/30—Devices for illuminating a surgical field, the devices having an interrelation with other surgical devices or with a surgical procedure
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/0012—Surgical microscopes
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/0092—Polarisation microscopes
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/18—Arrangements with more than one light path, e.g. for comparing two specimens
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/18—Arrangements with more than one light path, e.g. for comparing two specimens
- G02B21/20—Binocular arrangements
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Abstract
The invention discloses a dual-mode operation microscope for skin cancer treatment, which comprises a linearly polarized light source, a collimating lens, a band-pass filter, a liquid crystal polarization direction rotator, a large-field objective lens group, a spectroscope II, a spectroscope I, a spatial light modulator, a cylindrical lens II, a polarization detector, a spectroscope III, a cylindrical lens I and an ocular lens; the invention not only can realize the rapid and accurate diagnosis of skin cancer by using the large-field image and judge the range of tissue pathological changes, but also can use the local high-resolution polarization image to check the cancer cell distribution on the operation wound in the operation, thereby reducing unnecessary biopsy and improving the efficiency and the accuracy in the skin cancer excision operation process.
Description
Technical Field
The invention belongs to the technical field of microscopes, and particularly relates to a dual-mode operation microscope for skin cancer treatment.
Background
The method aims to solve the problems that the operation microscope at the present stage can not realize cancer cell detection in the operation and the small visual field positioning of the excision part is difficult. The existing methods have the contradiction between resolution and field of view, the field of view needs to be sacrificed to improve the resolution, and a cancer cell detection device in operation is lacked, the equipment switching is complicated, and the time and the precision are poor when the operation position is repeatedly positioned. Thus, the existing surgical microscope does not satisfy the requirement of accurate treatment of skin cancer.
Disclosure of Invention
In view of this, the present invention provides a skin cancer treatment-oriented dual-mode surgical microscope, which can achieve large-field local high-resolution fast polarization detection and dual-mode imaging of a visual surgical microscope.
The technical scheme for realizing the invention is as follows:
a dual-mode operation microscope facing skin cancer treatment comprises a linearly polarized light source, a collimating lens, a band-pass filter, a liquid crystal polarization direction rotator, a large-field objective lens group, a spectroscope II, a spectroscope I, a spatial light modulator, a cylindrical lens II, a polarization detector, a spectroscope III, a cylindrical lens I and an ocular lens;
the light beam output by the liquid crystal polarization direction rotator is refracted by a spectroscope II and irradiated to the surface of the skin through a large-field objective lens group of an object field, and the light reflected and scattered by the surface of the skin is collected by the large-field objective lens group and is divided into two paths by a spectroscope I;
the spatial light modulator modulates one path of light beams divided by the beam splitter I, so that the focal length of a local aperture is increased, and the local amplification of a large view field is realized; the light beam output by the spatial light modulator passes through the cylindrical lens II and then is imaged on the polarization detector, so that the polarization degree and polarization angle information of the image can be obtained, and the skin canceration and normal tissue boundary can be distinguished;
the other path of light beam split by the spectroscope I is refracted by the spectroscope III and converged by the cylindrical lens I; and finally, the light is divided into two paths through the ocular lens to be converged to human eyes.
Further, the linearly polarized light source consists of a white light source and a polaroid.
Further, by changing the input voltage of the liquid crystal polarization direction rotator, a continuous change of the polarization direction of the output light beam of at least 180 ° can be achieved.
Further, the polarization direction of the linearly polarized light beam is changed by changing the input voltage of the liquid crystal polarization direction rotator, so that a plurality of skin surface images with different polarization directions are finally obtained.
Furthermore, the polarization degree parameter has the capability of distinguishing skin canceration and normal tissue boundaries, and the detection aiming at the skin cancer can be realized through the analysis and the arrangement of the polarization degree information.
Has the advantages that:
the device not only can realize the rapid and accurate diagnosis of skin cancer by using the large-field image and judge the range of tissue pathological changes, but also can use the local high-resolution polarization image to check the distribution of cancer cells on the operation wound in the operation, thereby reducing unnecessary biopsy and improving the efficiency and the accuracy in the skin cancer excision operation process.
Drawings
Fig. 1 is a schematic structural diagram of a bimodal surgical microscope for skin cancer treatment according to the present invention.
The system comprises a 1-linearly polarized light source, a 2-collimating lens, a 3-band pass filter, a 4-liquid crystal polarization direction rotator, a 5-large field objective lens group, a 6-spectroscope II, a 7-spectroscope I, an 8-spatial light modulator, a 9-cylindrical lens II, a 10-polarization detector, a 11-spectroscope III, a 12-cylindrical lens I and a 13-eyepiece;
Detailed Description
The invention is described in detail below by way of example with reference to the accompanying drawings.
As shown in fig. 1, the present invention provides a local high-resolution dual-mode medical operating microscope, which comprises a linearly polarized light source 1, a collimating lens 2, a bandpass filter 3, a liquid crystal polarization direction rotator 4, a large-field objective lens group 5, a spectroscope II 6, a spectroscope I7, a spatial light modulator 8, a tube lens II 9, a detector 10, a spectroscope III 11, a tube lens I12, and an eyepiece 13;
the linearly polarized light source 1 consists of a white light source and a polaroid, a linearly polarized light beam emitted by the linearly polarized light source 1 is collimated through a collimating lens 2, filtered into light with the central wavelength of 532nm-575nm after passing through a band-pass filter 3, the polarization direction of output light can be continuously and rapidly changed by a liquid crystal polarization direction rotator 4 by changing input voltage, the continuous change of at least 180 degrees of the polarization direction of the output light beam can be realized, the light beam irradiates the surface of skin through a large-field objective lens group with an object space field of view of 170mm through a spectroscope II 6, light reflected and scattered by the surface of the skin is collected by the large-field objective lens group and is divided into two paths by a spectroscope I7;
the spatial light modulator 8 modulates one of the two light beams split by the beam splitter I7 to increase the local focal length of the light beam, so that the purpose of locally amplifying a large field of view is achieved, the interested part in skin cancer surgery is finely observed and operated, the light beam is imaged on the polarization detector 10 through the tube lens II 9 to obtain the polarization angle information of the image, and the change of the photon polarization state in the transmission process of the light on the surface of the skin is closely related to the microstructure of a skin medium, so that the light polarization state is measured to obtain the abundant structural information of the skin. The polarization degree parameter has the capability of distinguishing skin canceration from normal tissue boundaries, so that the detection aiming at the skin cancer can be realized through the analysis and the arrangement of the polarization degree information. However, the anisotropic structure in the biological tissue can affect the polarization measurement parameters, so that a plurality of images with different polarization directions can be obtained through the liquid crystal polarization direction rotator 4, and the influence of the anisotropic result on the polarization degree can be eliminated through analysis, so that the precise large-field local high-resolution variable polarization state detection is realized;
the cylindrical lens I12 divides the spectroscope I7, and the other path of light beam turned by the spectroscope III 11 is converged on an ocular lens 13, wherein the ocular lens 13 is a binocular stereoscopic ocular lens for visual observation, and the ocular lens 13 is used for imaging on human eyes to realize imaging of a visual observation system;
the visual observation is combined with the polarization imaging system, so that the rapid detection of the cancer cell edge can be simultaneously carried out in the skin cancer operation, and the accuracy of the operation can be greatly improved.
The actual working and running process of the system is as follows:
in the operation process, the large-view field objective lens group 5 is firstly utilized to quickly align the position to be operated, and the large-view field of the position to be operated is imaged on the detector 10, so that the position to be operated is quickly positioned. Then, a doctor carries out resection operation on the tumor through a visual observation system, simultaneously opens the linear polarization light source 1, irradiates an operation part by utilizing a polarization-variable illumination optical system, changes the polarization direction of a linear polarization light beam by changing the input voltage of the liquid crystal polarization direction rotator 4, thereby obtaining a plurality of images with different polarization directions, and analyzes the polarization degree information in the images, so that the influence of the orientation of the anisotropic structure in the sample on the imaging is eliminated. The spatial light modulator 8 in the large-field local high-resolution polarization detection system modulates the light path to increase the local focal length, so that the realized local high-resolution polarization imaging detects cancer cells on the edge of the operation wound to determine whether the tumor part is completely removed.
The skin cancer treatment-oriented dual-mode operation microscope provided by the embodiment of the invention has the following advantages:
1) the two modes of visual observation and polarization detection are seamlessly switched, so that the use is more convenient;
2) the liquid crystal polarization direction rotator is used for changing the polarization direction to obtain a plurality of images with different polarization angles, so that the influence of other anisotropic structures in skin tissues on the polarization degree can be eliminated, and the detection precision is improved;
3) local high-resolution high-magnification imaging in a large view field is realized, the view field and the magnification of an optical system can be considered, and the practicability of the system is improved.
In summary, the above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (4)
1. A bimodulus operating microscope facing skin cancer treatment is characterized by comprising a linearly polarized light source, a collimating lens, a band-pass filter, a liquid crystal polarization direction rotator, a large-field objective lens group, a spectroscope II, a spectroscope I, a spatial light modulator, a cylindrical lens II, a polarization detector, a spectroscope III, a cylindrical lens I and an ocular lens;
the linearly polarized light source consists of a white light source and a polaroid;
the light beam output by the liquid crystal polarization direction rotator is refracted by a spectroscope II and irradiated to the surface of the skin through a large-field objective lens group of an object field, and the light reflected and scattered by the surface of the skin is collected by the large-field objective lens group and is divided into two paths by a spectroscope I;
the spatial light modulator modulates one path of light beams divided by the beam splitter I, so that the focal length of a local aperture is increased, and the local amplification of a large view field is realized; the method comprises the following steps of carrying out fine observation and operation on an interested part in skin cancer operation, imaging a light beam output by a spatial light modulator on a polarization detector after passing through a cylindrical lens II, and acquiring polarization angle information of an image, wherein abundant structural information of skin can be obtained by measuring the polarization state of light due to the fact that the change of the photon polarization state of the light in the process of transmitting the light on the surface of the skin is closely related to the microstructure of a skin medium, so that the skin canceration and normal tissue boundaries can be distinguished;
however, the anisotropic structure in the biological tissue can affect the polarization measurement parameters, so that a plurality of images with different polarization directions can be obtained through the liquid crystal polarization direction rotator, and the influence of the anisotropic result on the polarization degree is eliminated through analysis, so that the accurate large-field local high-resolution variable polarization state detection is realized;
the other path of light beam split by the spectroscope I is refracted by the spectroscope III and converged by the cylindrical lens I; and finally, the light is divided into two paths through the ocular lens to be converged to human eyes.
2. The bimodal surgical microscope oriented to skin cancer treatment, as claimed in claim 1, wherein the continuous change of the polarization direction of the output beam of light by at least 180 ° is achieved by changing the input voltage of the liquid crystal polarization direction rotator.
3. The bimodal surgical microscope oriented to skin cancer treatment, as claimed in claim 2, wherein the polarization direction of the linearly polarized light beam is changed by changing the input voltage of the liquid crystal polarization direction rotator, thereby finally obtaining a plurality of skin surface images with different polarization directions.
4. The bimodal surgical microscope for skin cancer treatment as claimed in claim 1, wherein the polarization degree parameter has an ability to distinguish between skin cancerous and normal tissue boundaries, and detection of skin cancer is achieved by analysis and interpretation of the polarization degree information.
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| CN202011536365.8A CN112641520B (en) | 2020-12-22 | 2020-12-22 | Dual-mode operation microscope for skin cancer treatment |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP3642812B2 (en) * | 1994-11-17 | 2005-04-27 | 株式会社町田製作所 | Medical observation device |
| JP3373327B2 (en) * | 1995-04-24 | 2003-02-04 | 松下電器産業株式会社 | Foreign matter inspection device |
| US20060291042A1 (en) * | 2005-05-17 | 2006-12-28 | Alfano Robert R | Optical scanning zoom microscope with high magnification and a large field of view |
| DE102011088038B4 (en) * | 2011-12-08 | 2019-10-10 | Leica Microsystems (Schweiz) Ag | Surgical microscope system for ophthalmology and associated detection unit |
| CN103892919B (en) * | 2014-03-27 | 2016-03-30 | 中国科学院光电技术研究所 | Based on the microsurgical system that optical coherence tomography guides |
| CN104007559B (en) * | 2014-05-08 | 2017-05-17 | 北京理工大学 | Foveated imaging system with partial super-resolution scanning function |
| CN204287028U (en) * | 2014-11-15 | 2015-04-22 | 长春理工大学 | A kind of skin neoplasin imaging device |
| CN204839912U (en) * | 2015-07-08 | 2015-12-09 | 上海杰视医疗科技有限公司 | Ophthalmic surgery microscope with retina and cornea morphological analysis function |
| CN106707482A (en) * | 2017-02-28 | 2017-05-24 | 浙江大学 | Wide-view-field multi-scale high-resolution microimaging system and method |
| CN107632392B (en) * | 2017-08-22 | 2021-04-20 | 北京理工大学 | Dynamic local amplification high-resolution imaging system |
| CN109374590A (en) * | 2018-11-28 | 2019-02-22 | 西北大学 | A kind of hand-held skin canceration early stage optical detection apparatus and its application method |
| CN110584592B (en) * | 2019-09-09 | 2021-06-18 | 中国科学院苏州生物医学工程技术研究所 | Large-field-of-view adaptive optical retina imaging system and method for common-path beam scanning |
| CN110849849A (en) * | 2019-11-07 | 2020-02-28 | 南京理工大学 | Rapid modulation fluorescence polarization microscopic imaging device and method based on liquid crystal |
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