CN112626156A - Method for preparing collagen peptide by using circulating self-heating tank based on enzyme membrane coupling method - Google Patents
Method for preparing collagen peptide by using circulating self-heating tank based on enzyme membrane coupling method Download PDFInfo
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- 239000012528 membrane Substances 0.000 title claims abstract description 75
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 55
- 102000008186 Collagen Human genes 0.000 title claims abstract description 51
- 108010035532 Collagen Proteins 0.000 title claims abstract description 51
- 229920001436 collagen Polymers 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 35
- 238000010438 heat treatment Methods 0.000 title claims abstract description 33
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 25
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 25
- 238000010168 coupling process Methods 0.000 title claims abstract description 23
- 108091005804 Peptidases Proteins 0.000 claims abstract description 8
- 239000004365 Protease Substances 0.000 claims abstract description 8
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 8
- 239000000843 powder Substances 0.000 claims abstract description 8
- 238000006243 chemical reaction Methods 0.000 claims description 58
- 230000002572 peristaltic effect Effects 0.000 claims description 56
- 238000012216 screening Methods 0.000 claims description 37
- 239000012510 hollow fiber Substances 0.000 claims description 27
- 238000001914 filtration Methods 0.000 claims description 9
- 229920001661 Chitosan Polymers 0.000 claims description 6
- 101710127332 Protease I Proteins 0.000 claims description 6
- 101710137710 Thioesterase 1/protease 1/lysophospholipase L1 Proteins 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 239000011148 porous material Substances 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 5
- 239000000835 fiber Substances 0.000 claims description 4
- 101710180316 Protease 2 Proteins 0.000 claims description 3
- 238000001514 detection method Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 3
- 238000007710 freezing Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 abstract description 20
- 108090000623 proteins and genes Proteins 0.000 abstract description 20
- 230000035764 nutrition Effects 0.000 abstract description 6
- 235000016709 nutrition Nutrition 0.000 abstract description 6
- 238000010521 absorption reaction Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 239000003814 drug Substances 0.000 abstract description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 6
- 230000006872 improvement Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 2
- 108050001049 Extracellular proteins Proteins 0.000 description 1
- 108010093096 Immobilized Enzymes Proteins 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
Abstract
The invention relates to the technical field of medicine processing, in particular to a method for preparing collagen peptide by a circular self-heating tank based on an enzyme membrane coupling method. The method comprises the steps of arranging a circulating self-heating tank and preparing the collagen peptide with a certain molecular weight by using the circulating self-heating tank through an enzyme membrane coupling method, and finally preparing the collagen peptide with a certain molecular weight. The design of the invention can improve the enzymolysis effect of collagen, improve the yield of protease, improve the content of protein peptide in the finished product, reduce the nutrition loss, simultaneously improve the texture uniformity of the finished product powder of the collagen peptide, improve the quality of the finished product in appearance and taste, and facilitate the absorption of the nutrition of the protein peptide powder by human bodies.
Description
Technical Field
The invention relates to the technical field of medicine processing, in particular to a method for preparing collagen peptide by a circular self-heating tank based on an enzyme membrane coupling method.
Background
Collagen peptide is an extracellular protein consisting of two or more amino acids. The absorption of human body is carried out in a peptide mode, and the absorption utilization rate of edible protein peptide can reach 100%, so that the medicine or health care product taking collagen peptide as main component has high nutritive value, is convenient to be absorbed by human body, and reduces nutrition loss. However, in the processing process of collagen peptide products, the yield of protein peptide is often reduced due to poor enzymolysis effect, so that raw material waste and nutrition loss are caused; meanwhile, the existing collagen peptide product also has the problem that the quality of the finished product of the protein peptide powder is not fine and uniform enough due to uneven molecular weight distribution.
Disclosure of Invention
The invention aims to provide a method for preparing collagen peptide by a circulating self-heating tank based on an enzyme membrane coupling method, so as to solve the problems in the background technology.
In order to solve the above technical problems, an object of the present invention is to provide a method for preparing collagen peptide from a circulating self-heating tank based on an enzyme membrane coupling method, comprising the steps of:
s1, arranging a circulating self-heating tank;
s2, preparing collagen peptide with a certain molecular weight by using a circulating self-heating tank and an enzyme membrane coupling method.
2. The method for preparing collagen peptide by using the circulating self-heating tank based on the enzyme membrane coupling method according to claim 1, wherein: in S1, the method for setting the circulation self-heating tank includes the following steps:
s1.1, arranging a first reaction tank and a second reaction tank, wherein the reaction tanks can be automatically heated to 100 ℃ and keep a certain temperature constant, and meanwhile, arranging an automatic stirring device with adjustable rotating speed and a pH detection adjusting device;
s1.2, arranging a screening tank A, B, C, separating the screening tank by a fiber membrane with a certain molecular weight and pore diameter, and pressurizing the filter membrane to realize the molecular screening process;
s1.3, peristaltic pumps a-f are arranged to replace a pressurizing device to provide power for liquid inlet and liquid outlet.
As a further improvement of the present technical solution, in S2, the method for preparing collagen peptide with a certain molecular weight comprises the following steps:
s2.1, mixing collagen and water according to the weight ratio of 1:2, adding the mixture into a first reaction tank, adding immobilized protease I taking chitosan as a carrier into the first reaction tank, and adjusting the reaction temperature and the pH value to be optimum;
s2.2, after the first reaction tank reacts for a certain time, opening the peristaltic pumps a-c, adjusting the flow rate of the peristaltic pump a to be 200ml/min and the flow rates of the peristaltic pumps b and c to be 100ml/min, selecting a hollow fiber membrane I suitable for primary screening of large molecular weight, and selecting a filtration volume/membrane area ratio of 450ml/m2Pressurizing the filter membrane by 0.01MPa, adjusting the reaction temperature to be 37-40 ℃ and the pH value to be 7.0-7.5;
s2.3, adding immobilized protease (II) taking chitosan as a carrier into a second reaction tank, and adjusting the optimum reaction temperature and pH value to carry out secondary enzymolysis;
s2.4, opening the peristaltic pumps d to f after the peristaltic pump c is started for a certain time, adjusting the flow rate of the peristaltic pump d to be 100ml/min, adjusting the flow rate of the peristaltic pump e and the flow rate of the peristaltic pump f to be 50ml/min, selecting a hollow fiber membrane II, and selecting a filtration volume/membrane area ratio of 350ml/m2Pressurizing the filter membrane by 0.01MPa, adjusting the reaction temperature to be 37-40 ℃ and the pH value to be 7.0-7.5;
s2.5, selecting a hollow fiber membrane III, and filtering the mixture with a volume/membrane area ratio of 300ml/m2Pressurizing the filter membrane by 0.01MPa, adjusting the reaction temperature to be 37-40 ℃ and the pH value to be 7.0-7.5;
and S2.6, intercepting the collagen with the target molecular weight meeting the requirement, and freezing, drying and crushing the collagen to form powder.
As a further improvement of the technical scheme, the reaction conditions of 55 ℃ and pH 6.5-7.5 are set in the S2.1 and the S2.3.
As a further improvement of the technical scheme, the contents of the immobilized protease I and the immobilized protease II added in the S2.1 and the S2.3 are both 50U/mg.
The invention also aims to provide a circulating self-heating tank device based on an enzyme membrane coupling method, which comprises a first reaction tank, wherein a screening tank A, a second reaction tank, a screening tank B and a screening tank C are sequentially arranged on one side of the first reaction tank, a peristaltic pump a and a peristaltic pump B are arranged between the first reaction tank and the screening tank A, the running directions of the peristaltic pump a and the peristaltic pump B are opposite, a peristaltic pump C is arranged between the screening tank A and the second reaction tank, a peristaltic pump d and a peristaltic pump e are arranged between the second reaction tank and the screening tank B, the running directions of the peristaltic pump d and the peristaltic pump e are opposite, and a peristaltic pump f is arranged between the screening tank B and the screening tank C.
As a further improvement of the technical scheme, a hollow fiber membrane I is arranged in the middle of the screening tank A, a hollow fiber membrane II is arranged in the middle of the screening tank B, and a hollow fiber membrane III is arranged in the middle of the screening tank C.
As a further improvement of the technical scheme, the molecular weight and the pore diameter of the hollow fiber membrane I, the hollow fiber membrane II and the hollow fiber membrane III are sequentially reduced.
Compared with the prior art, the invention has the beneficial effects that: in this method of circulation self-heating jar preparation collagen peptide based on enzyme membrane coupling method, can carry out the circulation self-heating jar device that secondary enzymolysis and many times molecular sieve were strained through the setting, carry out secondary enzymolysis to collagen, can improve the enzymatic effect of collagen, improve the protease yield, improve the protein peptide content in the finished product, reduce the nutrition and run off, carry out a lot of sieves to protein peptide simultaneously, can improve the texture uniformity of collagen peptide finished product powder, improve the quality of finished product in outward appearance and taste, the human nutrition of protein peptide powder of being more convenient for of absorption.
Drawings
FIG. 1 is an overall process flow diagram of the present invention;
FIG. 2 is a flow diagram of a method of the present invention for providing a circulating autothermal tank;
FIG. 3 is a flow chart of a method for preparing collagen peptides with a certain molecular weight according to the present invention;
FIG. 4 is a schematic diagram of a circulating self-heating tank assembly of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Method embodiment
As shown in fig. 1 to 4, the present example is directed to a method for preparing collagen peptide from a circulating self-heating tank based on an enzyme membrane coupling method, comprising the steps of:
s1, arranging a circulating self-heating tank;
s2, preparing collagen peptide with a certain molecular weight by using a circulating self-heating tank and an enzyme membrane coupling method.
In this embodiment, in S1, the method for setting the circulation self-heating tank includes the following steps:
s1.1, arranging a first reaction tank and a second reaction tank, wherein the reaction tanks can be automatically heated to 100 ℃ and keep a certain temperature constant, and meanwhile, arranging an automatic stirring device with adjustable rotating speed and a pH detection adjusting device;
s1.2, arranging a screening tank A, B, C, separating the screening tank by a fiber membrane with a certain molecular weight and pore diameter, and pressurizing the filter membrane to realize the molecular screening process;
s1.3, peristaltic pumps a-f are arranged to replace a pressurizing device to provide power for liquid inlet and liquid outlet.
Wherein, the pH adjusting and detecting device arranged in the reaction tank is convenient for setting the most suitable reaction condition of the biological enzyme.
In this embodiment, in S2, the method for preparing collagen peptide with a certain molecular weight includes the following steps:
s2.1, mixing collagen and water according to the weight ratio of 1:2, adding the mixture into a first reaction tank, adding immobilized protease I taking chitosan as a carrier into the first reaction tank, and adjusting the reaction temperature and the pH value to be optimum;
s2.2, after the first reaction tank reacts for a certain time, opening the peristaltic pumps a-c, adjusting the flow rate of the peristaltic pump a to be 200ml/min and the flow rates of the peristaltic pumps b and c to be 100ml/min, selecting a hollow fiber membrane I suitable for primary screening of large molecular weight, and selecting a filtration volume/membrane area ratio of 450ml/m2Pressurizing the filter membrane by 0.01MPa, adjusting the reaction temperature to be 37-40 ℃ and the pH value to be 7.0-7.5;
s2.3, adding immobilized protease (II) taking chitosan as a carrier into a second reaction tank, and adjusting the optimum reaction temperature and pH value to carry out secondary enzymolysis;
s2.4, opening the peristaltic pumps d to f after the peristaltic pump c is started for a certain time, adjusting the flow rate of the peristaltic pump d to be 100ml/min, adjusting the flow rate of the peristaltic pump e and the flow rate of the peristaltic pump f to be 50ml/min, selecting a hollow fiber membrane II, and selecting a filtration volume/membrane area ratio of 350ml/m2Pressurizing the filter membrane by 0.01MPa, adjusting the reaction temperature to be 37-40 ℃ and the pH value to be 7.0-7.5;
s2.5, selecting a hollow fiber membrane III, and filtering the mixture with a volume/membrane area ratio of 300ml/m2Pressurizing the filter membrane by 0.01MPa, adjusting the reaction temperature to be 37-40 ℃ and the pH value to be 7.0-7.5;
and S2.6, intercepting the collagen with the target molecular weight meeting the requirement, and freezing, drying and crushing the collagen to form powder.
Wherein, through setting up two retort, can carry out the secondary enzymolysis to collagen, and then can fully degrade the collagen macromolecule.
Specifically, in S2.1 and S2.3, reaction conditions are set to be 55 ℃ and pH 6.5-7.5, and the conditions are suitable for immobilized protease reaction and improve the enzymolysis effect.
Wherein, the contents of the immobilized protease I and the immobilized protease II added in the S2.1 and the S2.3 are both 50U/mg.
Specifically, immobilized enzymes are added into the first reaction tank and the second reaction tank, so that the recycling of the biological enzymes can be ensured.
Comparative examples
In this example, the following two comparative experiments were performed on the method for enzymatic hydrolysis of collagen:
experiment 1
And simultaneously, adding a proper amount of immobilized protease into the first reaction tank and the second reaction tank, carrying out secondary enzymolysis, and carrying out tertiary screening.
Experiment 2
Only adding a proper amount of immobilized protease into the first reaction tank, carrying out primary enzymolysis, and carrying out three times of screening.
The preparation time, the amount of target protein peptide and the amount of residue of the above two experiments were compared, as shown in the following table:
| preparation time | Amount of target protein peptide | Amount of residue | |
| Experiment 1 | Long and long | Big (a) | Chinese character shao (a Chinese character of 'shao') |
| Experiment 2 | Short length | Small | Multiple purpose |
As can be seen from the above table, in the method for preparing collagen peptide with a certain molecular weight of the embodiment, the collagen is subjected to secondary enzymolysis, so that the enzymolysis effect of collagen can be enhanced, the yield of protein peptide can be increased, the uniformity of target protein peptide can be improved, and the quality of a finished product can be improved.
Apparatus embodiment
As shown in fig. 4, the present embodiment is directed to a circulating self-heating tank apparatus based on an enzyme membrane coupling method, comprising a first reaction tank, and a screening tank a, a second reaction tank, a screening tank B, and a screening tank C are sequentially disposed on one side of the first reaction tank.
In this embodiment, be equipped with peristaltic pump a, peristaltic pump b between first retort and the screening jar A, peristaltic pump a is opposite with peristaltic pump b's traffic direction, is equipped with peristaltic pump c between screening jar A and the second retort, and screening jar A sieves out the small molecular weight's protein peptide, carries the second retort and carries out the secondary enzymolysis, and the large molecular weight's protein peptide can get back to first retort and carry out enzymolysis once more.
Furthermore, a peristaltic pump d and a peristaltic pump e are arranged between the second reaction tank and the screening tank B, the running directions of the peristaltic pump d and the peristaltic pump e are opposite, a peristaltic pump f is arranged between the screening tank B and the screening tank C, the screening tank B screens out the protein peptides with smaller molecular weight, the protein peptides are conveyed to the screening tank C to be screened for the third time, and the protein peptides with larger molecular weight can return to the second reaction tank to be subjected to enzymolysis again.
Further, the screening tank C screens out protein peptides with smaller molecular weight, and uniform protein peptides with molecular weight cut-off between molecular weight B and molecular weight C are taken as target molecular weight protein peptides meeting the requirements.
In this embodiment, sieve and strain jar A in the middle of being equipped with hollow fiber membrane I, sieve and strain jar B in the middle of being equipped with hollow fiber membrane II, sieve and strain jar C in the middle of being equipped with hollow fiber membrane III.
Furthermore, the molecular weight and the pore diameter of the hollow fiber membrane I, the hollow fiber membrane II and the hollow fiber membrane III are sequentially reduced, and the fiber membranes with different cut-off molecular weights can ensure the uniformity of preparing collagen peptide with certain molecular weight.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the preferred embodiments of the present invention are described in the above embodiments and the description, and are not intended to limit the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (8)
1. A method for preparing collagen peptide by a circular self-heating tank based on an enzyme membrane coupling method is characterized by comprising the following steps: the method comprises the following steps:
s1, arranging a circulating self-heating tank;
s2, preparing collagen peptide with a certain molecular weight by using a circulating self-heating tank and an enzyme membrane coupling method.
2. The method for preparing collagen peptide by using the circulating self-heating tank based on the enzyme membrane coupling method according to claim 1, wherein: in S1, the method for setting the circulation self-heating tank includes the following steps:
s1.1, arranging a first reaction tank and a second reaction tank, wherein the reaction tanks can be automatically heated to 100 ℃ and keep a certain temperature constant, and meanwhile, arranging an automatic stirring device with adjustable rotating speed and a pH detection adjusting device;
s1.2, arranging a screening tank A, B, C, separating the screening tank by a fiber membrane with a certain molecular weight and pore diameter, and pressurizing the filter membrane to realize the molecular screening process;
s1.3, peristaltic pumps a-f are arranged to replace a pressurizing device to provide power for liquid inlet and liquid outlet.
3. The method for preparing collagen peptide by using the circulating self-heating tank based on the enzyme membrane coupling method according to claim 2, wherein: in S1, circulation self-heating jar device includes first retort, one side of first retort is equipped with sieve filter jar A, second retort, sieve filter jar B and sieve filter jar C in proper order, first retort with be equipped with peristaltic pump a, peristaltic pump B between the sieve filter jar A, peristaltic pump a with peristaltic pump B 'S traffic direction is opposite, sieve filter jar A with be equipped with peristaltic pump C between the second retort, the second retort with be equipped with peristaltic pump d, peristaltic pump e between the sieve filter jar B, peristaltic pump d with peristaltic pump e' S traffic direction is opposite, sieve filter jar B with be equipped with peristaltic pump f between the sieve filter jar C.
4. The method for preparing collagen peptide by using the circulating self-heating tank based on the enzyme membrane coupling method according to claim 3, wherein: the filter screen is characterized in that a hollow fiber membrane I is arranged in the middle of the filter screen A, a hollow fiber membrane II is arranged in the middle of the filter screen B, and a hollow fiber membrane III is arranged in the middle of the filter screen C.
5. The method for preparing collagen peptide by using the circulating self-heating tank based on the enzyme membrane coupling method according to claim 4, wherein: the molecular weight and the pore diameter of the hollow fiber membrane I, the hollow fiber membrane II and the hollow fiber membrane III are reduced in sequence.
6. The method for preparing collagen peptide by using the circulating self-heating tank based on the enzyme membrane coupling method according to claim 1, wherein: in S2, the method for preparing collagen peptide with a certain molecular weight comprises the following steps:
s2.1, mixing collagen and water according to the weight ratio of 1:2, adding the mixture into a first reaction tank, adding immobilized protease I taking chitosan as a carrier into the first reaction tank, and adjusting the reaction temperature and the pH value to be optimum;
s2.2, after the first reaction tank reacts for a certain time, opening the peristaltic pumps a-c, adjusting the flow rate of the peristaltic pump a to be 200ml/min and the flow rates of the peristaltic pumps b and c to be 100ml/min, selecting a hollow fiber membrane I suitable for primary screening of large molecular weight, and selecting a filtration volume/membrane area ratio of 450ml/m2Pressurizing the filter membrane by 0.01MPa, adjusting the reaction temperature to be 37-40 ℃ and the pH value to be 7.0-7.5;
s2.3, adding immobilized protease (II) taking chitosan as a carrier into a second reaction tank, and adjusting the optimum reaction temperature and pH value to carry out secondary enzymolysis;
s2.4, opening the peristaltic pumps d-f after the peristaltic pump c is started for a certain time, and adjusting the flow of the peristaltic pump d100ml/min, 50ml/min of flow rate of peristaltic pump e and f, selection of hollow fiber membrane II, 350ml/m of filtration volume/membrane area ratio2Pressurizing the filter membrane by 0.01MPa, adjusting the reaction temperature to be 37-40 ℃ and the pH value to be 7.0-7.5;
s2.5, selecting a hollow fiber membrane III, and filtering the mixture with a volume/membrane area ratio of 300ml/m2Pressurizing the filter membrane by 0.01MPa, adjusting the reaction temperature to be 37-40 ℃ and the pH value to be 7.0-7.5;
and S2.6, intercepting the collagen with the target molecular weight meeting the requirement, and freezing, drying and crushing the collagen to form powder.
7. The method for preparing collagen peptide by using the circulating self-heating tank based on the enzyme membrane coupling method according to claim 6, wherein: in S2.1 and S2.3, reaction conditions are set to 55 ℃ and pH6.5-7.5.
8. The method for preparing collagen peptide by using the circulating self-heating tank based on the enzyme membrane coupling method according to claim 6, wherein: and in the S2.1 and the S2.3, the contents of the immobilized protease I and the immobilized protease II are both 50U/mg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011408369.8A CN112626156A (en) | 2020-12-03 | 2020-12-03 | Method for preparing collagen peptide by using circulating self-heating tank based on enzyme membrane coupling method |
Applications Claiming Priority (1)
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| WO2013054363A1 (en) * | 2011-10-14 | 2013-04-18 | Council Of Scientific And Industrial Research | Peptides from fish gelatine |
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| CN110024901A (en) * | 2019-04-30 | 2019-07-19 | 集美大学 | A kind of collagen peptide and its production method and process units and application |
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| WO2013054363A1 (en) * | 2011-10-14 | 2013-04-18 | Council Of Scientific And Industrial Research | Peptides from fish gelatine |
| CN104762355A (en) * | 2015-03-04 | 2015-07-08 | 江苏大学 | Preparation method for fish scale collagen protein active peptide |
| CN105018554A (en) * | 2015-07-30 | 2015-11-04 | 北京天肽生物科技有限公司 | Small molecule bovine bone collagen peptide and preparation method thereof |
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