CN112625877B - Sorting and filtering device for tumor cell mass for oncology department - Google Patents
Sorting and filtering device for tumor cell mass for oncology department Download PDFInfo
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- CN112625877B CN112625877B CN202110107264.7A CN202110107264A CN112625877B CN 112625877 B CN112625877 B CN 112625877B CN 202110107264 A CN202110107264 A CN 202110107264A CN 112625877 B CN112625877 B CN 112625877B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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Abstract
The invention discloses a sorting and filtering device for tumor cell clusters for oncology, and relates to a tumor cell cluster filtering device which comprises a bottom plate, wherein lifting assemblies are fixedly connected above two sides of the bottom plate, lifting frames are fixedly connected above the lifting assemblies, a fixing frame which is in sliding connection with the lower portion of the middle portion of the lifting frames is fixedly connected in the middle of the bottom plate, a first swing rod is rotatably connected above the right side of the fixing frame, the upper end of the first swing rod is rotatably connected with an installation platform, the right side of the installation platform is rotatably connected with the upper portion of the right side of the lifting frames, a second swing rod is rotatably connected above the left side of the fixing frame, the upper end of the second swing rod is rotatably connected with a third swing rod, the upper end of the third swing rod is rotatably connected with the lower portion of the left side of the installation platform, a clamping assembly is fixedly connected above the installation platform, and a test tube is clamped by the clamping assembly. The lifting assembly is arranged above two sides of the bottom plate, the lifting frame can be driven to move up and down, and then the first swing rod, the second swing rod and the third swing rod above the fixing frame are matched to drive the installation table to swing, so that the test tube is driven to swing, and a vibration effect is achieved.
Description
Technical Field
The invention relates to a tumor cell mass filtering device, in particular to a sorting and filtering device for tumor cell masses in oncology.
Background
Tumor refers to a new organism formed by local tissue cell proliferation under the action of various tumorigenic factors, because the new organism mostly presents space-occupying block-shaped protrusions, which is also called neoplasm. According to the cellular characteristics of the new organism and the degree of harm to the organism, tumors are divided into two major categories, namely benign tumors and malignant tumors. Malignant tumors can be classified into carcinoma and sarcoma, and carcinoma refers to malignant tumor derived from epithelial tissue; sarcoma refers to mesenchymal tissue, including fibrous connective tissue, fat, muscle, vascular, bone, and cartilage tissue, among others.
When detecting the typing to tumor cell, need draw out tumor cell group from the tissue fluid usually, traditional screening mode generally is the in vitro setting screen cloth, and the test tube of stewing waits for its autosegregation screening, and it is very low to select separately filterable efficiency like this, can't satisfy the demand of short-term test.
For this reason, a sorting and filtering device for tumor cell masses for oncology has been proposed by those skilled in the art to solve the problems set forth in the background above.
Disclosure of Invention
The present invention is directed to provide a device for sorting and filtering tumor cell masses for oncology, which solves the problems set forth in the background art.
In order to achieve the purpose, the invention provides the following technical scheme:
a sorting and filtering device for tumor cell clusters for oncology comprises a base plate and a lifting assembly, wherein the lifting assembly is fixedly connected above two sides of the base plate, the lifting frame is fixedly connected above the lifting assembly, the middle part of the base plate is fixedly connected with a fixing frame which is in sliding connection with the lower part of the middle part of the lifting frame, the upper part of the right side of the fixing frame is rotatably connected with a first swing rod, the upper end of the first swing rod is rotatably connected with a mounting table, the right side of the mounting table is rotatably connected with the upper part of the right side of the lifting frame, the upper part of the left side of the fixing frame is rotatably connected with a second swing rod, the upper end of the second swing rod is rotatably connected with a third swing rod, the upper end of the third swing rod is rotatably connected with the lower part of the left side of the mounting table, a clamping assembly is fixedly connected above the mounting table, a test tube is clamped by the clamping assembly, a second filter tube is arranged above the test tube, the middle part of the second filter tube is fixedly connected with an inclined second filter layer, and two sides of the second filter tube are connected by an automatic limiting assembly, the second cartridge filter top has first cartridge filter through the bolt connection, first cartridge filter top fixedly connected with runner assembly, the first filter layer of runner assembly below fixedly connected with.
As a further scheme of the invention: the lifting assembly comprises a first motor, an oblique rotating wheel is fixedly connected to the middle of a first motor shaft fixedly connected to the upper portions of the two sides of the bottom plate, a limiting plate is rotatably connected to the upper end of the first motor shaft, a sliding shaft with the lower end sliding on the edge of the upper portion of the oblique rotating wheel is slidably connected to the other side of the limiting plate, and the upper end of the sliding shaft is fixedly connected with the lifting frame.
As a still further scheme of the invention: the centre gripping subassembly includes the mounting bracket, and fixed connection is at the mounting bracket fixedly connected with electric putter of mount table top, and electric putter tip fixedly connected with movable block, movable block both sides are rotated and are connected with two fourth pendulum rods, and the fourth pendulum rod other end is rotated and is connected with the sliding plate with mounting bracket sliding connection, sliding plate one side fixedly connected with grip block, and the centre gripping of depressed part has the test tube between the grip block.
As a still further scheme of the invention: automatic spacing subassembly includes the backup pad, and fixed connection is in backup pad fixedly connected with second motor of second cartridge filter both sides below, second motor shaft fixedly connected with gear, second cartridge filter both sides top fixedly connected with fixed plate, fixed plate sliding connection have with gear engagement's rack inserted bar, the rack inserted bar passes second cartridge filter and test tube edge.
As a still further scheme of the invention: the rotating assembly comprises a sealing cover, a support frame is fixedly connected to the upper portions of two sides of the sealing cover above the first filter cartridge in a threaded mode, a third motor is fixedly connected to the upper portion of the support frame, a cross rod is fixedly connected to the shaft of the third motor, and a connecting rod is fixedly connected to the lower end of the lower portion of the two ends of the cross rod and fixedly connected to the first filter layer.
As a still further scheme of the invention: and guide rods in sliding connection with the lifting frame are fixedly connected to the upper parts of the two sides of the bottom plate.
As a still further scheme of the invention: the middle part of the lifting frame is fixedly connected with a control unit.
As a still further scheme of the invention: and supporting legs are fixedly connected to the lower portions of the two sides of the bottom plate.
As a still further scheme of the invention: the fixing frame is connected with the bottom plate through bolts.
As a still further scheme of the invention: the middle part of the mounting table is provided with a groove matched with the bottom of the test tube.
Compared with the prior art, the invention has the beneficial effects that: the cell screening machine is novel in structure and convenient to operate, the lifting assemblies are arranged above two sides of the bottom plate and can drive the lifting frames to move up and down, and the first swing rod, the second swing rod and the third swing rod above the fixing frame are matched to drive the mounting table to swing, so that a test tube is driven to swing, a vibration effect is achieved, and the cell screening process can be accelerated; the preliminary filtration of cell also can be accelerated in the setting of pivoted first filter layer, and the second filter layer that the cooperation was inclined can promote screening efficiency.
Drawings
FIG. 1 is a schematic diagram of a device for sorting and filtering tumor cell masses for use in oncology;
FIG. 2 is a schematic diagram of a configuration at A of a device for sorting and filtering tumor cell masses for use in oncology;
FIG. 3 is a schematic diagram of a configuration at B in a device for sorting and filtering tumor cell masses for use in oncology;
FIG. 4 is a schematic view of the structure at C of a device for sorting and filtering tumor cell masses for use in oncology;
FIG. 5 is a sectional view of a mounting table of a sorting and filtering apparatus for tumor cell masses for oncology;
FIG. 6 is a schematic view of a clamping assembly of the device for sorting and filtering tumor cell mass for use in oncology;
in the figure: 1-bottom plate, 2-lifting component, 201-first motor, 202-oblique rotating wheel, 203-limiting plate, 204-sliding shaft, 3-lifting rack, 4-fixing rack, 5-first swinging rod, 6-mounting table, 7-second swinging rod, 8-third swinging rod, 9-clamping component, 901-mounting rack, 902-electric push rod, 903-moving block, 904-fourth swinging rod, 905-sliding plate, 906-clamping block, 10-test tube, 11-second filter cartridge, 12-second filter layer, 13-automatic limiting component, 1301-supporting plate, 1302-second motor, 1303-gear, 1304-fixing plate, 1305-rack inserted bar, 14-inserted bar, 15-first filter cartridge, 16-rotating component, 1601-cover, 1602-support frame, 1603-third motor, 1604-cross bar, 1605-connecting rod, 17-first filter layer, 18-guide rod, 19-control unit and 20-support leg.
Detailed Description
The technical solution of the present patent will be described in further detail with reference to the following embodiments.
The first embodiment is as follows: referring to fig. 1-6, a sorting and filtering device for tumor cell mass for oncology department, comprising a bottom plate 1, a lifting assembly 2, the lifting assembly 2 is fixedly connected above both sides of the bottom plate 1, a lifting frame 3 is fixedly connected above the lifting assembly 2, a fixing frame 4 slidably connected with the lower part of the middle part of the lifting frame 3 is fixedly connected in the middle of the bottom plate 1, a first swing rod 5 is rotatably connected above the right side of the fixing frame 4, a mounting table 6 with the right side rotatably connected with the upper part of the right side of the lifting frame 3 is rotatably connected with the upper end of the first swing rod 5, a second swing rod 7 is rotatably connected above the left side of the fixing frame 4, a third swing rod 8 with the upper end rotatably connected with the lower part of the left side of the mounting table 6 is rotatably connected with the upper end of the second swing rod 7, a clamping assembly 9 is fixedly connected above the mounting table 6, the clamping assembly 9 clamps a test tube 10, a second filter tube 11 is arranged above the test tube 10, an inclined second filter layer 12 is fixedly connected in the middle part of the second filter tube 11, the 11 both sides of second cartridge filter are connected through automatic spacing subassembly 13, and 11 tops of second cartridge filter are connected with first cartridge filter 15 through bolt 14, and first cartridge filter 15 top fixedly connected with rotating assembly 16, the first filter layer 17 of rotating assembly 16 below fixedly connected with.
The clamping assembly 9 comprises a mounting frame 901, an electric push rod 902 is fixedly connected to the mounting frame 901 above the mounting table 6, a moving block 903 is fixedly connected to the end of the electric push rod 902, two fourth swing rods 904 are rotatably connected to two sides of the moving block 903, the other ends of the fourth swing rods 904 are rotatably connected with a sliding plate 905 which is slidably connected with the mounting frame 901, a clamping block 906 is fixedly connected to one side of the sliding plate 905, and a test tube 10 is clamped between the clamping blocks 906 in a sunken position.
The automatic limiting assembly 13 comprises a supporting plate 1301, a supporting plate 1301 fixedly connected with a second motor 1302 fixedly connected to the lower portion of the two sides of the second filter cartridge 11, a gear 1303 fixedly connected to the shaft of the second motor 1302, a fixing plate 1304 fixedly connected to the upper portion of the two sides of the second filter cartridge 11, a rack inserting rod 1305 meshed with the gear 1303 and slidably connected to the fixing plate 1304, and the rack inserting rod 1305 penetrates through the edges of the second filter cartridge 11 and the test tube 10.
And guide rods 18 which are connected with the lifting frame 3 in a sliding manner are fixedly connected to the upper parts of the two sides of the bottom plate 1.
And supporting legs 20 are fixedly connected to the lower parts of the two sides of the bottom plate 1.
The fixing frame 4 is connected with the bottom plate 1 through bolts.
The middle part of the mounting table 6 is provided with a groove matched with the bottom of the test tube 10.
Example two: this embodiment is a further improvement of the previous embodiment: the crane 3 middle part fixedly connected with the control unit 19 is convenient for control each motor and electric putter 902's operation, guarantees that first filter layer 17 and second filter layer 12 carry out steady efficient filtration screening to the interstitial fluid in the test tube 10.
The working principle of the invention is as follows: the first motors 201 above two sides of the bottom plate 1 drive the oblique rotating wheels 202 to rotate, and further the sliding shafts 204 slide on the edges above the oblique rotating wheels 202 to drive the sliding shafts 204 to slide up and down, so as to drive the lifting frame 3 to move up and down, and further the first swing rods 5, the second swing rods 7 and the third swing rods 8 above the fixed frame 4 are matched to drive the mounting table 6 to swing, so that the test tube 10 is driven to swing, a vibration effect is achieved, and a cell screening process can be accelerated; the electric push rod 902 drives the moving block 903 to move, so as to drive the fourth swing rod 904 to swing, so as to drive the sliding plate 905 to slide, so that the test tube 10 is clamped by the clamping block 906, and the test tube 10 is prevented from falling; the second motor 1302 drives the gear 1303 to rotate, and further drives the rack inserted link 1305 to slide through gear engagement, so that the second filter cartridge 11 and the test tube 10 are fixedly connected; third motor 1603 drives horizontal pole 1604 and connecting rod 1605 and rotates, and then drives first filter layer 17 and rotates, and the preliminary filtration of cell can be accelerated in the setting of the first filter layer 17 of pivoted, and the second filter layer 12 that the cooperation was inclined can promote screening efficiency.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (7)
1. A sorting and filtering device for tumor cell masses for oncology department comprises a bottom plate (1), a lifting component (2), the lifting component (2) is fixedly connected above two sides of the bottom plate (1), and a lifting frame (3) is fixedly connected above the lifting component (2), and is characterized in that the middle part of the bottom plate (1) is fixedly connected with a fixing frame (4) which is in sliding connection with the lower part of the middle part of the lifting frame (3), a first swing rod (5) is rotatably connected above the right side of the fixing frame (4), the upper end of the first swing rod (5) is rotatably connected with a mounting platform (6) with the right side rotatably connected with the upper part of the right side of the lifting frame (3), a second swing rod (7) is rotatably connected above the left side of the fixing frame (4), a third swing rod (8) with the upper end rotatably connected with the lower part of the left side of the mounting platform (6) is rotatably connected with the upper end of the second swing rod (7), a clamping component (9) is fixedly connected above the mounting platform (6), the test tube (10) is clamped by the clamping component (9), a second filter cylinder (11) is arranged above the test tube (10), the middle part of the second filter cylinder (11) is fixedly connected with an inclined second filter layer (12), two sides of the second filter cylinder (11) are connected by an automatic limiting component (13), the upper part of the second filter cylinder (11) is connected with a first filter cylinder (15) by a bolt (14), a rotating component (16) is fixedly connected above the first filter cylinder (15), a first filter layer (17) is fixedly connected below the rotating component (16), the lifting component (2) comprises a first motor (201), an inclined rotating wheel (202) is fixedly connected to the middle part of the shaft of the first motor (201) fixedly connected above two sides of the bottom plate (1), a limiting plate (203) is rotatably connected to the upper end of the shaft of the first motor (201), and a sliding shaft (204) with the lower end sliding on the upper edge of the inclined rotating wheel (202) is slidably connected to the other side of the limiting plate (203), the upper end of the sliding shaft (204) is fixedly connected with the lifting frame (3), the automatic limiting assembly (13) comprises a supporting plate (1301), a second motor (1302) is fixedly connected with the supporting plate (1301) fixedly connected to the lower portion of the two sides of the second filter cartridge (11), a gear (1303) is fixedly connected with the shaft of the second motor (1302), a fixing plate (1304) is fixedly connected to the upper portion of the two sides of the second filter cartridge (11), a rack inserted bar (1305) meshed with the gear (1303) is slidably connected with the fixing plate (1304), the rack inserted bar (1305) penetrates through the edges of the second filter cartridge (11) and the test tube (10), the clamping assembly (9) comprises a mounting frame (901), an electric push rod (902) is fixedly connected with the mounting frame (901) above the mounting table (6), a moving block (903) is fixedly connected with the end portion of the electric push rod (902), and two fourth swing rods (904) are rotatably connected to the two sides of the moving block (903), the other end of the fourth swing rod (904) is rotatably connected with a sliding plate (905) which is in sliding connection with the mounting rack (901), one side of the sliding plate (905) is fixedly connected with clamping blocks (906), and test tubes (10) are clamped at the sunken parts between the clamping blocks (906).
2. The device for sorting and filtering tumor cell masses for oncology department according to claim 1, wherein the rotating assembly (16) comprises a cover (1601), a support frame (1602) is fixedly connected above two sides of the cover (1601) screwed above the first filter cartridge (15), a third motor (1603) is fixedly connected above the support frame (1602), a transverse rod (1604) is fixedly connected to the shaft of the third motor (1603), and a connecting rod (1605) is fixedly connected below two ends of the transverse rod (1604) and has a lower end fixedly connected with the first filter layer (17).
3. The device for sorting and filtering tumor cell mass for oncology department according to claim 1, wherein a guide rod (18) connected with the lifting frame (3) in a sliding way is fixedly connected above the two sides of the bottom plate (1).
4. The device for sorting and filtering tumor cell masses in oncology according to claim 1, wherein the crane (3) is fixedly connected with a control unit (19) in the middle.
5. The apparatus for sorting and filtering tumor cell mass for oncology department according to claim 1, wherein the bottom plate (1) is fixedly connected with supporting legs (20) below both sides.
6. The device for sorting and filtering tumor cell masses in oncology department according to claim 1, wherein the fixing frame (4) is connected with the bottom plate (1) by bolts.
7. The device for sorting and filtering tumor cell masses in oncology according to claim 1, wherein the mounting table (6) is provided with a groove in the middle for fitting the bottom of the test tube (10).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110107264.7A CN112625877B (en) | 2021-01-27 | 2021-01-27 | Sorting and filtering device for tumor cell mass for oncology department |
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| CN202110107264.7A CN112625877B (en) | 2021-01-27 | 2021-01-27 | Sorting and filtering device for tumor cell mass for oncology department |
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| CN112625877B true CN112625877B (en) | 2022-09-13 |
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| CN103604679A (en) * | 2013-11-25 | 2014-02-26 | 武汉友芝友医疗科技有限公司 | Capture instrument for circulating tumor cells and method for capturing and staining by using capture instrument |
| CN208302531U (en) * | 2018-05-11 | 2019-01-01 | 吉林大学 | A kind of separatory filter device of tumor blood |
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| CN111685777A (en) * | 2020-06-24 | 2020-09-22 | 魏长荣 | Circulating tumor cell separation and enrichment device based on closed loop |
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| CN112625877A (en) | 2021-04-09 |
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