CN112611779A - Method for rapidly detecting growing-suitable temperature range of cornus floribundus and application of method - Google Patents
Method for rapidly detecting growing-suitable temperature range of cornus floribundus and application of method Download PDFInfo
- Publication number
- CN112611779A CN112611779A CN202011238811.7A CN202011238811A CN112611779A CN 112611779 A CN112611779 A CN 112611779A CN 202011238811 A CN202011238811 A CN 202011238811A CN 112611779 A CN112611779 A CN 112611779A
- Authority
- CN
- China
- Prior art keywords
- cornus
- leaves
- floribundus
- floribunda
- temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000209020 Cornus Species 0.000 title claims abstract description 88
- 238000000034 method Methods 0.000 title claims abstract description 38
- 230000012010 growth Effects 0.000 claims abstract description 29
- 238000012360 testing method Methods 0.000 claims description 53
- 230000008859 change Effects 0.000 claims description 37
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 claims description 30
- 229930002875 chlorophyll Natural products 0.000 claims description 28
- 235000019804 chlorophyll Nutrition 0.000 claims description 28
- 238000002054 transplantation Methods 0.000 claims description 4
- 238000012216 screening Methods 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 2
- 238000005259 measurement Methods 0.000 abstract description 13
- 230000004075 alteration Effects 0.000 abstract description 3
- 230000035882 stress Effects 0.000 description 51
- 241000196324 Embryophyta Species 0.000 description 45
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 29
- 241000209022 Cornus florida Species 0.000 description 19
- 230000002829 reductive effect Effects 0.000 description 18
- 230000000694 effects Effects 0.000 description 16
- 230000007423 decrease Effects 0.000 description 14
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 13
- 229940118019 malondialdehyde Drugs 0.000 description 13
- 102000003992 Peroxidases Human genes 0.000 description 12
- 230000003247 decreasing effect Effects 0.000 description 12
- 102000019197 Superoxide Dismutase Human genes 0.000 description 11
- 108010012715 Superoxide dismutase Proteins 0.000 description 11
- 102000004190 Enzymes Human genes 0.000 description 10
- 108090000790 Enzymes Proteins 0.000 description 10
- 108040007629 peroxidase activity proteins Proteins 0.000 description 9
- 238000002835 absorbance Methods 0.000 description 8
- 230000029553 photosynthesis Effects 0.000 description 6
- 238000010672 photosynthesis Methods 0.000 description 6
- 230000000243 photosynthetic effect Effects 0.000 description 6
- 230000000630 rising effect Effects 0.000 description 6
- 230000004083 survival effect Effects 0.000 description 6
- 239000003086 colorant Substances 0.000 description 5
- XOFYZVNMUHMLCC-ZPOLXVRWSA-N prednisone Chemical compound O=C1C=C[C@]2(C)[C@H]3C(=O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 XOFYZVNMUHMLCC-ZPOLXVRWSA-N 0.000 description 5
- 230000009467 reduction Effects 0.000 description 5
- 238000006722 reduction reaction Methods 0.000 description 5
- 238000005070 sampling Methods 0.000 description 5
- 238000004364 calculation method Methods 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- LHGVFZTZFXWLCP-UHFFFAOYSA-N guaiacol Chemical compound COC1=CC=CC=C1O LHGVFZTZFXWLCP-UHFFFAOYSA-N 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000001035 drying Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 230000027756 respiratory electron transport chain Effects 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000759832 Cornus walteri Species 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000002845 discoloration Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 238000005562 fading Methods 0.000 description 2
- 229960001867 guaiacol Drugs 0.000 description 2
- 230000017525 heat dissipation Effects 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000003334 potential effect Effects 0.000 description 2
- 238000006862 quantum yield reaction Methods 0.000 description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 230000005068 transpiration Effects 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- RVBUGGBMJDPOST-UHFFFAOYSA-N 2-thiobarbituric acid Chemical compound O=C1CC(=O)NC(=S)N1 RVBUGGBMJDPOST-UHFFFAOYSA-N 0.000 description 1
- 241000864413 Cornus macrophylla Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 108010060806 Photosystem II Protein Complex Proteins 0.000 description 1
- 241001085296 Primula x polyantha Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000006851 antioxidant defense Effects 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 230000028514 leaf abscission Effects 0.000 description 1
- 230000002015 leaf growth Effects 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000004660 morphological change Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000008557 oxygen metabolism Effects 0.000 description 1
- 238000007540 photo-reduction reaction Methods 0.000 description 1
- 230000019935 photoinhibition Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000008288 physiological mechanism Effects 0.000 description 1
- 230000006461 physiological response Effects 0.000 description 1
- 230000005080 plant death Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000008261 resistance mechanism Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- MUUHXGOJWVMBDY-UHFFFAOYSA-L tetrazolium blue Chemical compound [Cl-].[Cl-].COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC=CC=2)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=CC=C1 MUUHXGOJWVMBDY-UHFFFAOYSA-L 0.000 description 1
- 238000004383 yellowing Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N25/00—Investigating or analyzing materials by the use of thermal means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N5/00—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid
- G01N5/04—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid by removing a component, e.g. by evaporation, and weighing the remainder
- G01N5/045—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid by removing a component, e.g. by evaporation, and weighing the remainder for determining moisture content
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N2021/8466—Investigation of vegetal material, e.g. leaves, plants, fruits
Abstract
The invention discloses a method for rapidly detecting the suitable temperature range of cornus floribunda and application thereof. The method comprises the following steps: determining the temperature range, and then measuring the influence of different temperatures on the growth indexes of the cornus floribundus, wherein the growth indexes comprise the shape of the leaves and the color difference of the leaves. According to the method, the high-temperature resistance of the cornus floribundus can be quickly and effectively determined by combining the state of the cornus floribundus leaves with the chromatic aberration measurement.
Description
Technical Field
The invention belongs to the field of plant planting, and particularly relates to a method for rapidly detecting a growth-suitable temperature range of cornus floribunda and application thereof.
Background
In recent years, researches on the aspects of ecological adaptability, variety screening, cultivation technology and the like of cornus floribunda are intensive, and the researches show that the cornus floribunda has stronger cold resistance, for example, annual seedlings of the cornus floribunda are not frozen and have no obvious plant diseases and insect pests in eastern China under the condition of no protective measures in winter, the cornus floribunda seedlings grow slower under the condition of proper climatic environment, and the growth rate of the seedlings is accelerated after 2 years. The flowers and the plants can also successfully live in summer after being introduced in Changzhou, Suzhou, Shanghai, Hangzhou and other places, so that the flowers and the plants have bright colors and unique flower shapes and particularly have ornamental values when being used as common colorful-leaf tree species with spring flowers, autumn flowers and rich seasons in the aspect of popularization and application of gardens, can be planted around courtyards, at the sides of water ponds and roads as flower shrubs to play a role in decorating landscapes, and have very wide application prospects in the future.
For the cornus floribunda, high temperature has greater influence on the growth of the cornus floribunda, and extreme high temperature is a main factor causing high growth limitation and long-branch deficit of the cornus floribunda. The influence of temperature on plants is manifold, for example, the optimum temperature of plant photosynthesis is slightly lower than the temperature when the photosynthesis is carried out fastest, the chlorophyll content of leaves is also an important index for directly reflecting the photosynthesis capability of the plants, and the change size and speed can also measure the physiological change condition of the leaves; the respiration of the plant is also affected by the temperature, and the respiration of the plant at night is weaker than that of the plant at day time due to the temperature cycle; the great influence of transpiration is air humidity which is influenced by temperature, and researches show that the closing degree of plant stomata and temperature are in negative correlation, so that the influence of extreme high temperature on the transpiration is shown in that the water consumption exceeds the water absorption of roots, and the plants can also suffer from wilting.
If the flowering dogwood is hot in summer and the high-temperature duration is long, the flowering dogwood which is wet and shade-resistant is difficult to pass through the summer, and growth conditions and physiological and biochemical reaction tests of the flowering dogwood under the high-temperature adverse stress condition have certain reference values for exploring a high-temperature-resistant physiological mechanism of the flowering dogwood and breeding high-temperature-resistant flowering dogwood varieties and popularizing and applying the flowering dogwood, so that the research on the high-temperature-resistant performance of the flowering dogwood has great significance for mining the potential value of the flowering dogwood and popularizing greening planting.
Disclosure of Invention
The purpose of the invention is as follows: the invention provides a method for rapidly detecting the growing-suitable temperature range of cornus floribunda, which can rapidly and effectively determine the high-temperature resistance of the cornus floribunda by combining the state of the cornus floribunda leaves with chromatic aberration measurement. The invention also provides application of the method in the transplantation of cornus floribunda, and the transplantation survival rate can be greatly improved.
The technical scheme is as follows: the invention relates to a method for rapidly detecting the suitable temperature range of cornus floribunda, which comprises the following steps of: the temperature range is determined, and then the influence of different temperatures on the growth index of the cornus floribundus is measured.
In the method for detecting the growth suitability temperature range of cornus floribundus, the growth indexes comprise leaf morphology.
In the method for detecting the growth suitability temperature range of the cornus floribundus, the growth indexes comprise leaf color difference.
In the method for detecting the growth adaptive temperature range of cornus floribundus, growth indexes comprise chlorophyll content, fluorescence kinetic parameters and physiological and biochemical indexes.
The method for detecting the growth-suitable temperature range of cornus floribundus comprises the following steps of:
(1) determining the high-temperature resistant range of the cornus floribunda through a screening test;
(2) setting a temperature gradient, and culturing the cornus floribunda under different temperature conditions;
(3) measuring the color difference and the shape of leaves, grading the shapes of the leaves of the cornus floribundus grown under different temperature conditions, and determining the influence degree of the cornus floribundus on high temperature;
(4) measuring the chlorophyll content, and determining the influence of different temperatures on the chlorophyll content of cornus floridulus;
(5) measuring fluorescence kinetic parameters, and determining the change rule of the fluorescence kinetic parameters of the cornus floribunda at different temperatures;
(6) and measuring physiological and biochemical indexes, and determining the change rule of the cornus floribundus under different high temperatures.
(7) And determining the high-temperature resistance of the cornus floribunda.
The method for rapidly detecting the suitable temperature range of the cornus floribundus walsingl for growing the cornus floribundus walsingl is applied to transplanting of the cornus floribundus walsingl.
The leaf grade of the cornus floribunda leaves which can be transplanted is not higher than grade II.
The blade class determination index is: including the grade of the blade morphology and the grade of the blade color difference.
The method for judging the grade of the blade form comprises the following steps:
stage I: leaf blade fullness degree: plump; leaf color: normal; other external phenomena of the blade: no wilting or burning phenomenon;
and II, stage: leaf blade fullness degree: the mixture is relatively full; leaf color: basically normal; other external phenomena of the blade: withering or burning in small area
Grade III: leaf blade fullness degree: partially shrinking; leaf color: green and light; other external phenomena of the blade: the phenomenon of wilting or burning appears in large area;
IV stage: leaf blade fullness degree: large-area shrinkage; leaf color: severe discoloration; other external phenomena of the blade: severe water loss and withering;
and V stage: leaf blade fullness degree: completely shrinking; leaf color: complete color fading; other external phenomena of the blade: all scorched.
The method for judging the grade of the blade chromatic aberration comprises the following steps:
stage I: a: -6.44 to-7.43, b: 13.25 to 13.73;
and II, stage: a: -6.55 to-7.82, b: 13.21 to 15.38;
grade III: a: -6.66 to-0.26, b: 13.70-12.55; IV stage: a: -0.26 to-1.57, b: 12.55 to 11.23;
and V stage: a: -1.57, b: < 12.1.
Has the advantages that: (1) according to the method, the high-temperature resistance of the cornus floribundus can be rapidly determined by combining the morphological characteristics of leaves of the cornus floribundus and the color difference value; (2) according to the method, the high-temperature resistance of the cornus floribunda can be more accurately judged by combining other physiological indexes of the cornus floribunda, and the growth state of the cornus floribunda is judged; (3) the method can determine the popularization range and the temperature adaptation area of various varieties of the cornus floribunda, and avoids the occurrence of unnecessary loss caused by blind popularization of the cornus floribunda in the application process; (4) the method can quickly determine whether the flowering dogwood can be transplanted, if leaves of the flowering dogwood are in the level II, the flowering dogwood can grow normally, and if the leaves of the flowering dogwood are in the level III, the flowering dogwood cannot be planted.
Drawings
FIG. 1 shows the morphology and color variation of Cornus walteri leaves;
FIG. 2 is a detail morphology change of cornus florida leaves observed by a microscope;
fig. 3 is a change in the edge morphology of cornus florida leaves observed by a microscope;
FIG. 4 shows the changes of Δ a, Δ b, Δ L, and Δ E under high temperature stress;
FIG. 5 shows the water content of cornus polyandra under high temperature stress;
FIG. 6 shows the change of the relative chlorophyll content of cornus floridulus under high temperature stress;
FIG. 7 shows florida cornus chlorophyll fluorescence F under high temperature stress0Variation of Fm
FIG. 8 shows the changes of florida cornus chlorophyll fluorescence Fv/Fm and Fv/Fo under high temperature stress;
FIG. 9 shows the change of physiological and biochemical indicators of Cornus polyandra under high temperature stress.
Detailed Description
Materials and methods
1.1 test materials and sites
The materials selected in the high-temperature stress test are annual multi-flowered cornus macrophylla potted seedlings with strong plants and good growth conditions and originated seeds, the growth heights of the seedlings are basically consistent, the weight of each pot is about 6kg (containing sandy loam and having the organic matter content of 15.827g/kg), the number of leaves exceeds 80 (containing no tender leaves), 1 plant is planted in each pot, 30 pots are processed for 3 times, and the whole pot seedling stress test period is carried out in a full-automatic intelligent artificial climate box.
1.2 test apparatus
A multifunctional full-automatic artificial climate box, a CM-700d/600d spectrocolorimeter, an SPAD chlorophyll content tester, a Handy PEA high-speed continuous excitation type fluorometer, an ultra-low temperature refrigerator, a centrifuge, a Mettler ML204 model one day per ten thousand and the like.
1.3 design of the experiment
1.3.1 preliminary experiments
And performing initial stress tests on potted seedlings in 7-9 months in 2019 to draft two groups. According to the introduction conditions of China and the planting conditions of Japan, the set temperature is respectively 35 ℃ and 40 ℃, the reaction change of the plant leaves in the artificial climate box along with the stress is observed, the corresponding average survival days are respectively 34d and 24d, and the results are shown in tables 1 and 2.
TABLE 135 deg.C temperature stress test of average survival days of Cornus polyandra seedlings
TABLE 240 ℃ temperature stress test average survival days of Cornus florida seedlings
1.2.2 stress test
Then, according to the survival days of the pre-test plants, the stress starting temperature is determined to be 35 ℃, the final temperature is determined to be 45 ℃, and the high-temperature stress test is carried out on 14 days in 10 months in 2019. The test is arranged into two groups and is carried out simultaneously, T is 30 pots of test group (high temperature stress of 35-45 ℃), 3 times are repeated, and CK is a control group (35 ℃) is kept unchanged; the experimental design used a single factor fully random variable (table 3). Before the test, soil balls of the cornus polyandra plants are wrapped by soft water-permeable non-woven bags with the same size as the canopy width of potted seedlings, trays with the same radius are padded at the bottoms of the potted seedlings, water is timely watered to keep the water in the trays, and the water balance of the plants is kept by utilizing the siphonage capacity of soil to the water.
TABLE 3 Single-factor completely random variable test design
The method comprises the following steps of (1) beginning an experiment, placing potted seedlings in an artificial climate box, keeping the interval between two adjacent plants at about 5cm, reducing the mutual influence between the plants as much as possible, adjusting initial environmental parameters in the climate box, adjusting the illuminance to 3000lx, carrying out light treatment for 14h, carrying out dark treatment for 10h (for simulating the change of the length of day and night in summer in northern hemisphere), keeping other environmental factors unchanged, stressing the initial temperature to 35 ℃, then increasing the temperature by 2d at intervals, and when the temperature reaches 39 ℃, increasing the temperature by 1 ℃ at intervals of 2d (according to the climate characteristics of the middle and lower reaches of Yangtze river, keeping the temperature of 39 ℃ in a high-temperature state, relieving the physiological influence of the high temperature on the plants, enabling the plants to adapt to the high temperature more easily, facilitating the further proceeding of. During the test, index determination and leaf sampling work are carried out every 2d, the index determination and leaf sampling work is taken as 8 o' clock at night, leaf samples selected during determination are all selected from the middle part of each potted seedling, samples for water content determination come from the base part of the plant, 1-3 leaves with the same part of different plants are selected during sampling work, 20 leaves are selected in total, the leaf samples are immediately placed into a sealing bag after sampling is finished, serial numbers are marked, and the leaf samples are wrapped by ice bags and placed in an ultra-low temperature refrigerator of minus 180 ℃ for sealing and storage for later-period sample measurement.
Second, test determination index and method
2.1 measurement of leaf growth morphology and color index
The external growth form of the plant is represented by observing and recording the form change of the leaves, and the appearance form of the leaves is divided into 5 grades according to the growth condition and the appearance quality of the leaves. Wherein, I level: the blood is full and normal; and II, stage: the mixture is full and normal; grade III: dehydrating and shrinking; IV stage: severe water loss shrinkage; and V stage: completely dehydrated and scorched (see table 4 for details).
TABLE 4 vane appearance morphology grades
The shape and color indexes (chromaticity) of the leaves are subjected to color difference measurement by adopting a spectrocolorimeter CM-700d/600d, the leaves with large upper fixed positions in the plant are selected, the corrected spectrocolorimeter is used for measuring the upper positions of the leaves of the fixed leaves, the data of the leaves measured for the first time are recorded as initial standard colors, L (illumination/brightness), a (red-green value) and b (yellow-blue value) of the initial standard colors are three parameters of the initial standard colors, the data obtained by each measurement are contrast colors (contrast parameters), and delta L, delta a and delta b are used as corresponding color difference indexes. Δ E is the total color difference change. Wherein the delta E has small change between 0 and 0.5; the variation is 0.5-2.0; 2.0-4.0 is large in variation; if the value is 4.0 or more, the variation is very large.
Calculating the formula:
2.2 measurement of moisture content in leaves
The water content of the leaves is determined by a drying and weighing method. Selecting leaves with proper size at the bottom of the plant, sampling, taking 3 leaves from each plant, firstly weighing the mass of the leaves after being picked, and recording as the fresh weight m1 of the leaves; then putting all weighed blades into distilled water, fully soaking for 5h, taking out and wiping off the water on the surfaces of the blades, and recording the saturated mass of the blades as m 2; and finally, placing the leaves in a drying box at 105 ℃ for deactivation of enzymes for 30min, then baking the leaves in the drying box at 80 ℃ for 12h until the weight of the leaves is constant, and measuring the dry weight of the leaves, and recording the dry weight as the dry weight m3 of the leaves. The water content of the blade (LWC) and the relative water content of the blade (LRWC) are calculated through formulas.
The calculation formula is as follows:
2.3 leaf chlorophyll content determination
Chlorophyll content determination process: selecting the same leaf on the middle upper part of the same plant, measuring the relative content of chlorophyll by using a SPAD chlorophyll meter every two days, measuring the same leaf for 5 times, taking an average value as the content of chlorophyll, recording data and comparing the change of the relative content of chlorophyll along with the temperature rise of the test.
2.4 fluorescence kinetic parameter determination
Fluorescence kinetic parameter determination process: measuring chlorophyll fluorescence parameters by using a Handy PEA high-speed continuous excitation type fluorometer, selecting a fixed healthy leaf part to perform 30min metal sheet dark-light reaction before measurement, and repeating the measurement work for 3 times each time, wherein the measured green fluorescence parameter indexes of the leaf comprise initial fluorescence (Fo), maximum fluorescence (Fm), maximum quantum efficiency (Fv/Fm) of a PS II system and PS II quantum efficiency (Fv/Fo).
Calculating the formula:
2.5 measurement of physiological and biochemical indices
The physiological and biochemical indexes tested by the test comprise superoxide dismutase (SOD), Peroxidase (POD) and Malondialdehyde (MDA), which are chemical substances existing in plant cells, and the index content of the physiological and biochemical indexes is closely related to the oxygen metabolism activity of plants, the aging and the stress resistance of plants. The measurement method is as follows:
the superoxide dismutase (SOD) activity is determined by using a Nitrogen Blue Tetrazolium (NBT) photoreduction method to inhibit NBT photochemical reduction so as to determine the enzyme activity, wherein the SOD activity unit is expressed by taking 50% of the inhibition of NBT photochemical reduction as an enzyme activity unit; wherein A0: absorbance of the control tube under light; as: measuring the absorbance of the tube by using the sample; vt: total sample extract volume (mL); vs: taking the amount (mL) of crude enzyme solution during measurement; fw: fresh weight of sample (g).
The calculation formula is as follows:
peroxidase (POD) activity assay: measured by guaiacol colorimetry with hydrogen peroxide (H)2O2) Measuring the absorbance change of the product after guaiacol oxidation at 470nm, and recording the increase of 0.01 of A470nm per minute as 1 enzyme activity unit (U); wherein Δ A470: change of absorbance value under 470nm wavelength; vt: total volume (mL) of extracted enzyme solution; vs: taking the volume (mL) of enzyme solution during measurement; fw: fresh weight of sample (g).
The calculation formula is as follows:
malondialdehyde (MDA) content determination: measuring by thiobarbituric acid method (TBA), and calculating absorbance values A450, A532 and A600 of centrifuged supernatant of Cornus walteri specimen at 450nm, 532nm and 600nm on a spectrophotometer according to a formula. Wherein A450: absorbance value at wavelength of 450 nm; a532: absorbance value at 532nm wavelength; a600: absorbance value at 600nm wavelength; vt: total volume (mL) of extracted enzyme solution; fw: fresh weight of sample (g).
The calculation formula is as follows:
third, experimental results
3.1 Effect of high temperature stress on growth morphology of Cornus florida leaves
From fig. 1 to 3, it can be seen that the external growth morphology of cornus polyandra leaves is changed significantly under high temperature stress, and specifically, the leaf appearance is grade i under the stress of the initial temperature of 35 ℃ at the 2 nd day: the leaves are normal in color, full and complete, and have no wilting or burning phenomenon; 4d to 8d, the stress temperature is between 37 and 40 ℃, the leaf color is basically normal, the leaves are plump, the wilting or burning phenomenon only appears on small areas or leaf edges, and the appearance of the leaves is level II; 10d, when the stress temperature reaches 41 ℃, the external growth morphology of the leaves is greatly changed, the leaves are green and light, the leaves are shriveled, the phenomena of wilting or burning appear in a large area, vital signs are still present, and the appearance of the leaves is grade III; and 12-14 d in the stress test, the appearance of the blade is IV grade: the leaves are seriously dehydrated and withered; to 16d, when at the high temperature of 44 ℃, the blade appearance is class v: leaves are completely dehydrated, completely scorched and completely lose vital signs. Compared with the stress test group T, the symptoms of the cornus floridulus leaves in the control group are not changed greatly (Table 5), and a little withered and back-rolling phenomenon only appears at the leaf edges after 16 d.
When the 14 th stress temperature reaches 41 ℃, the stress test leaves are watershed and have vital signs, and before the stress test leaves have normal color and are full; then, the leaves of the cornus floridulus are changed greatly, and are quickly dehydrated, shrunk and faded until the leaves are completely dehydrated and die.
TABLE 5 symptom Change of Cornus polyanthus leaves after high temperature stress
Note: blank indicates normal; + indicates the same symptom. a represents stain; b represents leaf discoloration; c represents leaf abscission or withering; d indicates plant death.
The color difference index of the blade measured by the color spectrometer comprises three parameters of a (red-green value), b (yellow-blue value) and L (illumination/brightness), and contrast parameters delta a, delta b and delta L. When a, b and L are positive values, if delta a is a positive value, the leaf color is red compared with the standard color a under the initial temperature stress, otherwise, the leaf color is greenish; if the delta b is a positive value, the leaf color is yellow compared with the standard b color under the initial temperature stress, otherwise, the leaf color is blue; if delta L is a positive value, the color of the leaf is brighter than the standard L color under the initial temperature stress, otherwise, the color is darker.
The color difference index change result is shown in fig. 4, wherein both delta a and delta L are in rising trend, the rising amplitude of delta a is maximum, and the rising amplitude is 6.18NBS higher than the maximum peak value of the initial a (red-green value); the rising amplitude of delta L is small, and only 1.81NBS is increased in 16d of the stress test; and deltab shows a slow descending trend, and the minimum value of the deltab is only reduced by 2.02NBS compared with the initial b (yellow-blue value); therefore, in the stress test, the leaves of the cornus floribunda turn red to blue, namely, the green degree gradually becomes lighter, and the shade of the leaves tends to be stable. The total color difference change Delta E is basically between the normal change and the larger change at 10d (35-41 ℃) before the test, and the change at the later 6d (42-44 ℃) is very large.
The three indexes of delta a, delta b and delta L have small overall change between 37 ℃ and 41 ℃, slightly fluctuate within a certain small range, and the overall trend is relatively stable. Except for delta L, the change values of delta a and delta b account for 16.05 percent and 44.45 percent of the total change value of each; the delta a and the delta b are greatly changed between 41 ℃ and 44 ℃, wherein the rising value of the delta a accounts for 83.95% of the total rising value during the period; Δ b is 55.55%, and Δ E likewise varies in value between 37 ℃ and 41 ℃ by 33.97% of the total variation.
According to the legend, the change trends of the four indexes of the leaf color difference delta a, delta b, delta L and delta E are basically consistent with the change of the external growth form of the leaf, the change is divided into two stages by taking 41 ℃ at the 10 th stage as a boundary, the external change of the leaf is not obvious in the first stage (the first 10d of stress), only the edge is slightly scorched and brown spots, the leaf color is only slightly changed, the green color is slightly lightened, the leaf pulp is plump, has certain elasticity, and the overall vital signs are obvious; in the second stage (the last 10 days of stress), the leaves of cornus floribunda have a large amount of scorched brown spots, the color of the leaves is changed greatly, the green color is changed into yellow and brown rapidly, the edges of the leaves are rolled reversely, the water loss is reduced, the leaves are crisp and hardened until the leaves die completely.
3.2 Effect of high temperature stress on Water content of Cornus florida leaves
During the test, in order to reduce other factors except the temperature of the cornus floribunda in the artificial climate box, water is supplemented to potted seedlings at regular intervals, the siphon effect of soil is utilized, the water of the plants is kept basically balanced, the temperature of the plants rises at the later stage, the evaporation of the water is accelerated, and the interval time of water supplement is further shortened for continuously keeping the microenvironment of the rhizosphere and the supply of the water of the plants. FIG. 5 shows that the control CK showed no significant change at 35 ℃ in either LWC (leaf water content) or LRWC (leaf relative water content), essentially decreasing by about 10% in the initial water content value; while in the test group T of high temperature stress, LWC and LRWC showed overall downward trend as the test proceeded. With the 8d (40 ℃) as a boundary, the LWC and the LRWC have slower descending trends, namely, the LWC and the LRWC descend 23 percent and 23.2 percent respectively, and after the 8d, the LWC and the LRWC greatly descend 45.6 percent and 33.9 percent respectively. Particularly, the LRWC value is linearly reduced to 42.2 percent from 72.8 percent from the 8 th d to the 10 th d (40 ℃ to 41 ℃), and is reduced by 30.6 percentage points; meanwhile, the LWC value is reduced by 16.8 percentage points; both indices reached a minimum of about 20% water content at 14d (43 deg.C), after which there was a small range of lift back at 16d (44 deg.C).
3.3 influence of high temperature stress on chlorophyll content of Cornus polyandra leaves
Chlorophyll content measurement results as shown in fig. 6, in the 16d test, the relative chlorophyll content of the test group T and the control group CK decreased to different degrees with the test. The whole test T and CK respectively reduce from 59.59 zigzag to 23.52, 63.5 steadily to 53.41, and the relative value of each spad is reduced by 36.07 and 10.09. The florida dogwood test group is bounded by 8d (40 ℃), and the small amplitude of the first stage (from 2d to 8d) is reduced by 8.8 spad chlorophyll units, which only accounts for 20% of the total reduction range; the second stage (8 d to 16d) was a large drop of 27.2 span chlorophyll units, accounting for 80% of the total drop; especially at 10d (41 ℃), the relative content of chlorophyll is reduced by 10.75 units every day, and then the relative content is reduced by about 5 units every day until 16d reaches the minimum value of 23.52, the content of chlorophyll in leaves is low, and the photosynthesis capacity of plants is almost zero.
3.4 Effect of high temperature stress on floristicus cornus leaves fluorescence kinetic parameters
The fluorescence parameters of plants are the three ways of consuming chlorophyll to absorb light energy, and the three ways of photosynthetic electron transfer and heat dissipation are balanced, and the change of the chlorophyll fluorescence parameters can reflect the basic condition of plant photosynthesis. Fo fixed fluorescence (initial fluorescence), as the fluorescence yield when the photosystem II (PS II) reaction center is fully open, can reflect the electron transfer through the system; fm maximum fluorescence, which is the fluorescence yield when the reaction center of PS II is completely closed, can reflect the electron transfer condition of PS II system[19]。
As shown in FIG. 7, the overall stress test at 16d showed a substantially steady upward trend of Fo, except for a slight decrease at 12 d; the Fm continuously decreases along with the extension of the high-temperature stress time, the total amount of the Fm decreases by 1408 units, the 8d (40 ℃) and the 12d (42 ℃) are taken as boundary points, the Fm decreases by 525 units in the first stage from the 2d to the 8d, the second stage from the 8d to the 12d, the 12d to the 16d, and the third stage by 242 units, the decreasing ranges of the three stages respectively account for 37%, 46% and 17% of the total decreasing range, the changes of the first stage and the second stage are relatively gentle, and the decreasing range of the second stage is the largest; in general, there is generally no major fluctuation in the magnitude of the initial fluorescence and the maximum fluorescence, and the continuous increase in Fo and the continuous decrease in Fm reflect the continuous decrease in net photosynthetic rate (Pn) of the psii system; further, the fluorescence capability of the cornus floridulus leaves is reduced, the photochemical quantum yield is obviously reduced, the energy consumption of a heat dissipation way in a photosynthetic system is obviously reduced, and certain irreversible damage is generated on a leaf photosynthesis reaction mechanism by high-temperature stress.
The variable fluorescence (Fv) is the difference between Fm and Fo, the ratio (Fv/Fm) of the variable fluorescence Fv to the maximum fluorescence Fm is the maximum photochemical quantum yield of the PS II, the ratio (Fv/Fo) of the variable fluorescence Fv to the initial fluorescence Fo is the chlorophyll photosynthetic potential activity, the growth change amplitude of the two indexes is very small under the non-stress condition of the plant, the two indexes are obviously reduced under the stress condition, and particularly the reduction of the Fv/Fm is an important parameter for judging whether photoinhibition occurs or not. The test results are shown in FIG. 8, the Fv/Fm decreases with the increasing number of test days from the initial 0.81 to 0.57, wherein the decrease from 8d to 10d is the largest, the ratio decreases by about 0.13, which is about 41% of the total decrease range; the photosynthetic latent activity (Fv/Fo) decreased by 3.40 during the test, and the Fv/Fo decreased by 1.16 in the first stage from 2d to 8d, accounting for about 34% of the total decrease, bounded by 8d (40 ℃) and 10d (42 ℃); the descending amplitude is maximum in the second stage from 8d to 10d, and 1.44 units of descending accounts for about 43 percent of the whole descending range; the final third stage of the reduction was slow, only 0.79 units, and was about 23% overall. The decrease of the two parameters in different amplitudes further indicates that the PS II system is damaged, the light energy capturing capability and the potential activity of the PS II are continuously reduced, and the reaction center is seriously damaged under the high-temperature stress.
3.5 influence of high temperature stress on physiological and biochemical indexes inside cornus floribunda
The determination results of the contents of SOD (superoxide dismutase), POD (peroxidase) and MDA (malondialdehyde) of cornus florida under high-temperature stress are shown in FIG. 9, which is a high-temperature stress test in a period of 16d, wherein the general trend of the SOD enzyme activity index is firstly reduced and then increased, the content of 6dSOD enzyme is reduced by about 35% before the first stage from 2d to 6d by taking the 8d (40 ℃) as a boundary, and the minimum value of the SOD enzyme content in the whole test is reached at the 6 d; then, in the next 10d of the second stage of the test, the activity is steadily increased, but the content is not changed greatly from the 10 th d to the 16 th d, and no obvious difference exists, so that the SOD enzyme is not excessively involved in an antioxidant mechanism in the early stage of the reaction, and the activity of the SOD enzyme is increased because the plant reaction center ensures the normal metabolism of the plant body in the later stage.
The POD (peroxidase) activity under high-temperature stress results show that after the 16d high-temperature test, the POD enzyme content shows a trend of decreasing and increasing along with the prolonging of the test treatment time, the POD enzyme content of the first 6d is decreased to the lowest value of the 6d at a decreasing rate of about 30% per day with the 8d (40 ℃) as a boundary, the content is increased continuously and reaches the highest value, and the POD enzyme content is increased by 43.9% and further decreased at the 16d compared with the POD value at the initial level.
The result of the content of MDA (malondialdehyde) under high temperature stress shows that through a 16d high temperature test, the MDA enzyme content also has the trend of decreasing firstly and increasing secondly along with the time extension, the content increases by 15 percent in the front and back, and the difference between the lowest value of the 8d and the highest value of the 14d is about 10 units; the MDA content continuously decreases from the first stage from the 2d stage to the 8d stage (35 ℃ -40 ℃), the MDA content steadily increases after the 8d stage, the MDA content decreases and increases before and after the test, a response resistance mechanism for reacting cornus floribunda at high temperature has certain hysteresis, and the hysteresis is particularly shown in the aspect of physiological response; when the antioxidant defense measures are started in the later stage, MDA secretion is further stimulated, the content is obviously increased, and active oxygen in cells is greatly damaged by high temperature.
According to the index change performance of the cornus floribunda in the test process, the external form, particularly leaves of the cornus floribunda under the high-temperature stress are changed greatly. The external form of the leaf does not change obviously at 6d from the beginning of the test, the 10 th d is taken as a boundary, the leaf color of the plant is basically normal before the test, the appearance of the leaf is kept in the I-II grade, only part of the leaf edge has the yellowing phenomenon, the whole body is still full of vitality, the 10 th plant still has vitality, the external form of the leaf is continuously deteriorated after the 10 th (41 ℃) is carried out, the fading phenomenon is aggravated, and the leaf is atrophied; the water content and the chlorophyll content in the body are greatly reduced at the 8 th day, and experiments show that the PS II reaction center of the cornus floribunda is destroyed at high temperature, so that the fluorescence capability of leaves is reduced, and excessive light energy is grown, so that the chlorophyll photosynthetic mechanism is further destroyed, and the plant body is damaged; the critical date of internal relevant physiology change is slightly earlier than the critical date of external morphological change of the plant.
According to the test results, the stage that the changes of the shape and the color of the cornus floribunda are most obvious from 40 ℃ to 41 ℃ and the period that the changes of various biochemical indexes are most severe is also the period, so that the temperature of 40 ℃ is determined to be the critical temperature for the survival of the cornus floribunda, and when the temperature reaches 41 ℃, the leaf sheets of the cornus floribunda are quickly withered and even fall off, and the physiological and biochemical aspects are seriously created. Therefore, the leaves of the cornus floribunda can grow normally when the leaves are in the level II (the shape and the color difference of the leaves are in the level II), and the cornus floribunda cannot be planted when the leaves are in the level III.
Claims (8)
1. A method for rapidly detecting the growing-suitable temperature range of cornus floridulus is characterized by comprising the following steps: the temperature range is determined, and then the influence of different temperatures on the growth index of the cornus floribundus is measured.
2. The method for rapidly identifying the cornus floribunda suitable growth temperature range according to claim 1, wherein the growth indicator comprises leaf morphology.
3. The method for rapidly identifying the cornus floribunda suitable growth temperature range according to claim 2, wherein the growth indicator comprises leaf color difference.
4. The method for rapidly testing the suitable growing temperature range of cornus polyandra as claimed in claim 3, wherein the growth indexes comprise chlorophyll content, fluorescence kinetic parameters and physiological and biochemical indexes.
5. The method for rapidly detecting the cornus floribunda suitable growing temperature range according to claim 4, which is characterized by comprising the following steps of:
(1) determining the high-temperature resistant range of the cornus floribunda through a screening test;
(2) setting a temperature gradient, and culturing the cornus floribunda under different temperature conditions;
(3) measuring the color difference and the shape of leaves, grading the shapes of the leaves of the cornus floribundus grown under different temperature conditions, and determining the influence degree of the cornus floribundus on high temperature;
(4) measuring the chlorophyll content, and determining the influence of different temperatures on the chlorophyll content of cornus floridulus;
(5) measuring fluorescence kinetic parameters, and determining the change rule of the fluorescence kinetic parameters of the cornus floribunda at different temperatures;
(6) and measuring physiological and biochemical indexes, and determining the change rule of the cornus floribundus under different high temperatures.
(7) And determining the high-temperature resistance of the cornus floribunda.
6. The method for rapidly testing the growing suitable temperature range of cornus floribundus as claimed in claim 1, for use in the transplantation of cornus floribundus.
7. Use according to claim 6, characterized in that the leaf grade of cornus floribundus usable for transplantation is not higher than grade II.
8. Use according to claim 7, wherein the blade classes comprise classes of blade morphology and classes of blade colour difference.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011238811.7A CN112611779A (en) | 2020-11-09 | 2020-11-09 | Method for rapidly detecting growing-suitable temperature range of cornus floribundus and application of method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011238811.7A CN112611779A (en) | 2020-11-09 | 2020-11-09 | Method for rapidly detecting growing-suitable temperature range of cornus floribundus and application of method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112611779A true CN112611779A (en) | 2021-04-06 |
Family
ID=75224568
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011238811.7A Pending CN112611779A (en) | 2020-11-09 | 2020-11-09 | Method for rapidly detecting growing-suitable temperature range of cornus floribundus and application of method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112611779A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104237191A (en) * | 2014-10-09 | 2014-12-24 | 南京林业大学 | Method for quickly determining light intensity and temperature adaptation range of plants and device thereof |
| CN105550162A (en) * | 2015-12-10 | 2016-05-04 | 南京信息工程大学 | Method for determining high temperature stress grades of grape plants |
| CN109164212A (en) * | 2018-09-08 | 2019-01-08 | 华中农业大学 | A kind of measuring method using excised leaf identification Chinese rose germplasm heat resistance |
| CN110470565A (en) * | 2019-08-27 | 2019-11-19 | 江苏农林职业技术学院 | A method of measurement flowering dogwood drought-resistance ability |
| CN110568128A (en) * | 2019-09-12 | 2019-12-13 | 江苏农林职业技术学院 | method for measuring waterlogging tolerance of cornus floribunda |
| CN110632258A (en) * | 2019-09-26 | 2019-12-31 | 江苏农林职业技术学院 | Method for determining salt tolerance of cornus floribunda |
-
2020
- 2020-11-09 CN CN202011238811.7A patent/CN112611779A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104237191A (en) * | 2014-10-09 | 2014-12-24 | 南京林业大学 | Method for quickly determining light intensity and temperature adaptation range of plants and device thereof |
| CN105550162A (en) * | 2015-12-10 | 2016-05-04 | 南京信息工程大学 | Method for determining high temperature stress grades of grape plants |
| CN109164212A (en) * | 2018-09-08 | 2019-01-08 | 华中农业大学 | A kind of measuring method using excised leaf identification Chinese rose germplasm heat resistance |
| CN110470565A (en) * | 2019-08-27 | 2019-11-19 | 江苏农林职业技术学院 | A method of measurement flowering dogwood drought-resistance ability |
| CN110568128A (en) * | 2019-09-12 | 2019-12-13 | 江苏农林职业技术学院 | method for measuring waterlogging tolerance of cornus floribunda |
| CN110632258A (en) * | 2019-09-26 | 2019-12-31 | 江苏农林职业技术学院 | Method for determining salt tolerance of cornus floribunda |
Non-Patent Citations (7)
| Title |
|---|
| 张文会等: "18个冬小麦品种幼苗的高温耐受性比较研究", 《广西农业科学》 * |
| 李合生: "《植物生理生化实验原理和技术》", 31 July 2000 * |
| 杨丙贤: "水杨酸对温度胁迫下紫御谷幼苗根系生长、光合及生理特性的影响", 《CNKI硕士电子期刊》 * |
| 杨子平等: ""6种装配式墙体绿化植物的耐热性研究"", 《林业与环境科学》 * |
| 王彦芹等: "沙漠植物花花柴幼苗对高温耐受性评价", 《生物技术通报》 * |
| 陈景震: "美国梾木引种栽培果实落果+生理生化分析研究", 《湖南林业科技》 * |
| 马晓娣等: ""作物耐热性的评价"", 《植物学通报》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wilson | Variation in leaf respiration in relation to growth and photosynthesis of Lolium | |
| Koornneef et al. | Photomorphogenic responses of long hypocotyl mutants of tomato | |
| Bish et al. | Development of containerized strawberry transplants for Florida's winter production system | |
| Nakashima et al. | Photoprotective function of betacyanin in leaves of Amaranthus cruentus L. under water stress | |
| Zhu et al. | Morphological and photosynthetic response of waxy corn inbred line to waterlogging | |
| CN114451282A (en) | Indoor rapid identification and screening method for salt and alkali-resistant oats | |
| Kong et al. | Response of photosynthetic parameters of sweet pepper leaves to light quality manipulation by photoselective shade nets | |
| Kobori et al. | Supplemental light with different blue and red ratios in the physiology, yield and quality of Impatiens | |
| Kycheryavyi et al. | The Influence of Climatic and Edaphic Conditions on the Development of Thuja occidentalis' Smaragd'Under the Urban Conditions of a Large City | |
| Bruelheide et al. | Experimental tests for determining the causes of the altitudinal distribution of Meum athamanticum Jacq. in the Harz Mountains | |
| Huang et al. | Chilling injury in mature leaves of rice. I. Varietal differences in the effects of chilling on canopy photosynthesis under simulated'dry cold dew wind'conditions experienced in south-east China | |
| CN115504828B (en) | Method for improving low-calcium stress resistance of white gourd | |
| CN112611779A (en) | Method for rapidly detecting growing-suitable temperature range of cornus floribundus and application of method | |
| CN110679402A (en) | Method for improving cold resistance of zoysia japonica | |
| CN114774300B (en) | Pseudomonas koraiensis and application thereof | |
| Świerczyński et al. | Influence of rootstocks and the time of grafting procedure on the efficiency of propagation by grafting two cultivars of mountain pine (Pinus mugo Turra) and estimation of chloroplast pigments level in the needles | |
| CN115266617A (en) | Screening method of low-temperature-resistant garden chrysanthemum varieties in plateau area | |
| CN109197490A (en) | A method of improving tree peony heat resistance | |
| Gu et al. | Physiological responses of tung tree (Vernicia fordii) saplings to different red, white and blue light-emitting diodes. | |
| Ghaderi et al. | The effect of water stress on some physiological characteristics in'rashe'and'khoshnave'grape cultivars | |
| CN107660355A (en) | A kind of seed pre-sowing treatment method for improving winter wheat seedlings drought-resistant ability | |
| CN113016518A (en) | Resistance identification method for celery leaf spot | |
| CN116008196B (en) | Method for rapidly and minimally invasively detecting activity of young garlic fruits | |
| CN115380808B (en) | Method for identifying peanut germplasm iron sensitivity in iron absorption aspect | |
| CN111492829B (en) | Open-field over-summer method for alum roots in Shanghai region |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210406 |