CN110679402A - Method for improving cold resistance of zoysia japonica - Google Patents
Method for improving cold resistance of zoysia japonica Download PDFInfo
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- CN110679402A CN110679402A CN201911104959.9A CN201911104959A CN110679402A CN 110679402 A CN110679402 A CN 110679402A CN 201911104959 A CN201911104959 A CN 201911104959A CN 110679402 A CN110679402 A CN 110679402A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G20/00—Cultivation of turf, lawn or the like; Apparatus or methods therefor
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Abstract
The invention discloses a method for improving the cold resistance of zoysia japonica, belonging to the technical field of lawn planting, wherein zoysia japonica is potted or potted, grown for one month, cultured in an artificial climate incubator under the illumination of 16 hours at 24 ℃ and the illumination intensity of 2000-3000 mu mol.m‑2·s‑1And then culturing for 15-20 days at 4 ℃ for 8h in the dark. The zoysia japonica planted by the method can be used in northern areas of China (such as Beijing, Lanzhou and the like, the lowest annual temperature is higher than-20 ℃) for more than 220 days in the green period, and the zoysia japonica can safely pass the winter.
Description
Technical Field
The invention belongs to the technical field of lawn planting, and particularly relates to a method for improving the cold resistance of zoysia japonica.
Background
Zoysia is an important lawn grass species in China, not only is an excellent lawn plant and a good soil-fixing slope-protecting plant, but also has high feeding value. Zoysia is a warm-land type lawn grass species, is a good ecological type lawn grass due to its great tolerance to extensive management, and is gradually planted in regions north of the Yangtze river of China. But because of poor cold resistance, it is difficult to safely overwinter in some northern areas. The invention provides a method for effectively improving the cold resistance of zoysia plants.
Disclosure of Invention
The purpose of the invention is as follows: provides a method for improving the cold resistance of zoysia japonica, which solves the problem that the zoysia japonica is not suitable for being planted in cold regions in the prior art.
The technical scheme is as follows: in order to solve the technical problems, the invention adopts the following technical scheme:
a method for improving the cold resistance of zoysia japonica comprises planting zoysia japonica in pot or hole tray, culturing in artificial climate incubator at 24 deg.C for 16 hr under illumination intensity of 2000-3000 μmol.m-2·s-1And then culturing for 15-20 days at 4 ℃ for 8h in the dark.
The method for improving the cold resistance of zoysia japonica comprises the following steps:
(1) low-temperature exercise: planting zoysia japonica in a pot or a plug, after growing for one month, performing low-temperature exercise, culturing with a climatic incubator at 24 deg.C for 16h with illumination intensity of 2000-3000 μmol · m-2·s-1Then culturing for 15-20 days at 4 ℃ for 8h at night;
(2) building a terrace: planting the zoysia japonica treated by the low-temperature exercise in the step (1) on a soil terrace bed according to a conventional method, wherein the planting time in the northern area is 5-8 months;
(3) n, P, K fertilization management: n, P, K compound fertilizer is applied twice in 6-8 months, the interval between two times of application is 30 days, and the weight ratio of N, P, K in the N, P, K compound fertilizer is 4: 1: 6-4: 2: 7, preferably 4: 1: 6;
(4) exogenous hormone ABA (abscisic acid) administration: applying ABA twice within 8-9 months at an interval of 15-20 days, wherein the concentration of the ABA is 5-10mg/LABA, and preferably 10 mg/L; the application amount is 100-150ml/m2Preferably 100ml/m2。
Has the advantages that:
the invention discloses a method for improving the cold resistance of zoysia japonica, the zoysia japonica planted according to the method can be planted in northern areas of China (such as Beijing, Lanzhou and the like, the annual lowest temperature is higher than-20 ℃) for more than 220 days in the green period, and the zoysia japonica can safely pass winter.
Drawings
FIG. 1 change in proline content under low temperature stress.
FIG. 2 variation of chlorophyll a content.
FIG. 3 variation of chlorophyll b content.
Detailed Description
Example 1:
1. low-temperature exercise: planting zoysia plant in pot or hole tray, growing for one month, performing low temperature exercise, culturing with artificial climate incubator at 24 deg.C for 16 hr with illumination intensity of 2000-3000 μmol · m-2·s-1Then culturing for 15-20 h at 4 ℃ in the dark for 8hWatering normally in the day and in the middle;
2. building a terrace: the zoysia japonica treated by low-temperature exercise is planted on the soil terrace bed according to a conventional method, and the zoysia japonica is preferably planted in 5-8 months in northern areas.
3, N, P, K fertilization management: applying N, P, K compound fertilizer twice in 6-8 months, wherein the interval between two fertilizing operations is 30 days, and the weight ratio of N element, P element and K element in N, P, K compound fertilizer is 4: 1: 6-4: 2: 7;
4. exogenous hormone ABA (abscisic acid) administration: applying ABA twice within 8-9 months at an interval of 15-20 days, wherein the concentration of the ABA is 5-10mg/LABA, and the application amount is 100-150ml/m2。
Example 2:
1 materials and methods
The selected zoysia japonica with excellent properties was subjected to treatment A1 (treated and planted according to the treatment method of example 1), treatment A2 (the treatments 1, 2 and 3 were not performed, and the other planting management was the same as A1), and A3 (the treatment of the treatment 1 was not performed, and the other planting management was the same as A1). All three treatments were carried out in Beijing, 2016, 7 months.
1.2 Observation of Green phase
The selected grass seeds are sown in the experimental land in a one-to-one correspondence mode, unified management is tried during planting, watering and weeding are carried out regularly, the experimental grass is guaranteed to have the same growth environment, and the growth conditions are almost the same and good. From turning green in the early spring of 2017 to withering yellow in autumn, the green conditions of different treatments are observed and recorded.
1.4 Low temperature stress tolerance treatment
The three treated grass species were subjected to experimental potting in two portions in 7 months in 2017. Culturing the first part at room temperature, measuring physiological index, treating the other part in a freezer at low temperature, regulating the temperature to 2-3 deg.C, watering at regular time every day, selecting leaves at intervals of 2, 4 and 6 days to measure mda content and chlorophyll content, repeating for three times, and standing the plant at room temperature for growth recovery observation after low temperature test.
1.5 evaluation of Low temperature resistance
1.5.1 morphological Observation
And (3) after the selected grass seeds are placed in a refrigerator, recording the color and the growth state of the stems and leaves of the grass seeds at different periods of stress treatment.
1.5.2 determination of chlorophyll
Grinding 0.05g of selected phyllanthus niruri plants in a mortar, adding a proper amount of quartz sand into the mortar, adding a proper amount of 70% acetone solution during grinding, finishing grinding when the leaves of the experimental grass are ground into liquid, adding 10ml of alcohol (95%) solution into the liquid again for constant volume, completely wrapping the liquid by using a black plastic bag, standing in the shade, extracting 1ml of upper solution and a cuvette after two days, taking alcohol as reference groups, and carrying out colorimetric detection at 645nm and 663nm respectively
1.5.3 determination of proline
Weighing 0.1g of sample, placing the sample into a test tube, adding 1ml of extracting solution, sealing the opening of the test tube, extracting the sample for 10min in a 95-degree water bath, centrifuging the sample for 10min at 25 ℃ again at 10000g, absorbing 0.5 ml of supernate after cooling, adding 0.5 ml of reagent II and 0.5 ml of glacial acetic acid into the test tube with a cover, placing the test tube in a 95-degree water bath for 30min, shaking the test tube once every 10min, adding 1ml of methylbenzene after cooling, shaking the test tube for 30s to transfer pigment into a reagent III, absorbing 0.8ml to 1ml of upper solution into a 1ml glass cuvette, and carrying out colorimetric reaction at 520nm
1.5.4 calculation
Measuring chlorophyll content
Ca(mg·L-1)＝12.25A663-2.55A645
Cb(mg·L-1)＝20.31A645-4.91A663
CT(mg·L-1)＝Ca+Cb
Measurement of pro content
Typical regression equation y-0.0521 x-0.0021(x is proline content,. mu.g/ml; y is absorbance A)
Measuring mda content
MDA content (nmol/g fresh weight) [ [ △ a × V anti-total ÷ (epsilon × d) × 109]25.8 × △ a/W (W × v/v total)
Data analysis
Data analysis was performed with span.
2 results and analysis
2.1 Observation of Green phase
In the lawn, the appearance starts to be green when the greenness reaches 50%, and therefore, it is generally considered that turning green starts when the greenness exceeds 50%, and conversely, the turning yellow starts.
TABLE 1 Green period record Table
| A1 | A2 | A3 | |
| Green period (sky) | 223 | 185 | 192 |
Observations of the log found that treatment a1 had a significantly longer green period than the other treatments.
2.2 proline content Change under Low temperature stress
Proline is an important low-molecular compound of plants, when the plants are stressed by adversity such as low temperature, saline alkali and the like, free proline in the plants can be accumulated, and when the plants are recovered to a normal environment, the proline becomes an essential substance for plant recovery. It is generally considered that the higher the content is, the stronger the cold resistance is, and the weaker the content is, as shown in fig. 3, the lower the normal content of a1 is, the higher the A3 is, the higher the a2 is, the cold resistance a1> A3> a2 is at normal temperature, after the low-temperature treatment, the 30% -40% increase of the three test grass plants is, which should be due to the adaptive reaction of the plant body to the stress, after 48h the a1 and the A3 adapt to the low-temperature environment, the slow increase is, the loss of the synthetic raw materials is probably due to the decrease of the physiological activity frequency, after 96h the increase is again obvious, the proline accumulation is caused by the imbalance of the plant in the severe environment for a long time, the proline of the a 2-like plant is always in the greatly increased state, even 85% increase appears after 48h, but on the other hand, the increase of proline also reflects the damage degree of the plants, and the m18 sample is inspected after the stress treatment is completed to find that the severe withering phenomenon occurs, the growth restoring capability of the m18 sample is influenced, and therefore, the cold resistance capability of the m18 sample is poor.
2.3 chlorophyll Change
The content of a pigment in chloroplasts of chlorophyll plants for photosynthesis is directly related to the content of chloroplasts, the difference of chlorophyll of three varieties before stress treatment is mainly due to different greenness, during the whole treatment process, the chlorophyll a and the chlorophyll b of the varieties are in a descending state, so that the chloroplast in the plants is always in a reducing state under the action of low-temperature environment and is accumulated with damage along with the increase of time, the chlorophyll (a and b) content of A1 before the experiment is started is similar to an A3 index, and is expanded after the experiment is ended, and the A2 is seriously reduced along with the increase of extreme environment time, and the damage degree of the chlorophyll is obviously seen from the appearance.
From top to bottom, the cold resistance is arranged from strong to weak: a1> A3> A2.
Claims (6)
1. A method for improving the cold resistance of zoysia japonica is characterized in that the zoysia japonica is potted or potted and planted in a hole tray, after growing for one month, the zoysia japonica is cultured in a climatic incubator and is illuminated for 16 hours at 24 ℃, and the illumination intensity is 2000-3000 mu mol.m-2·s-1And then culturing for 15-20 days at 4 ℃ for 8h in the dark.
2. A method of improving the cold resistance of zoysia japonica according to claim 1, comprising the steps of:
(1) low-temperature exercise: planting zoysia japonica in a pot or a plug, after growing for one month, performing low-temperature exercise, culturing by using an artificial climate incubator, irradiating for 16h at 24 ℃, and then culturing for 15-20 days at 4 ℃ at night for 8 h;
(2) building a terrace: planting the zoysia japonica treated by the low-temperature exercise in the step (1) on a soil terrace bed according to a conventional method, wherein the planting time in the northern area is 5-8 months;
(3) n, P, K fertilization management: applying N, P, K compound fertilizer twice in 6-8 months, wherein the interval between the two fertilizing is 30 days;
(4) exogenous hormone ABA administration: ABA is applied twice within 8-9 months, and the interval between the two times is 15-20 days.
3. The method for improving the cold resistance of zoysia japonica according to claim 2, wherein the illumination intensity in the step (1) is 2000 to 3000 μmol-m-2·s-1。
4. The method for improving the cold resistance of zoysia japonica according to claim 2, wherein in the step (3), the mass ratio of the N element to the P element to the K element in the N, P, K compound fertilizer is 4: 1: 6-4: 2: 7.
5. the method for improving the cold resistance of zoysia japonica according to claim 2, wherein the concentration of the ABA in the step (4) is 5-10 mg/LABA.
6. The method for improving cold resistance of zoysia japonica according to claim 5, wherein ABA is applied in an amount of 100-150ml/m2。
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113079998A (en) * | 2021-05-08 | 2021-07-09 | 四川农业大学 | Method for improving cold resistance of red bud pineapples |
| CN114732016A (en) * | 2022-05-18 | 2022-07-12 | 江苏农林职业技术学院 | Preparation for promoting growth of creeping side buds of zoysia macrosperma in severe saline-alkali soil and using method thereof |
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| CN1876813A (en) * | 2006-06-06 | 2006-12-13 | 北京农业生物技术研究中心 | Method for improving cold resistance of zoysia |
| CN106613243A (en) * | 2016-12-23 | 2017-05-10 | 江苏农林职业技术学院 | Method for enabling zoysia japonica to turn green earlier |
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2019
- 2019-11-13 CN CN201911104959.9A patent/CN110679402A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1876813A (en) * | 2006-06-06 | 2006-12-13 | 北京农业生物技术研究中心 | Method for improving cold resistance of zoysia |
| CN106613243A (en) * | 2016-12-23 | 2017-05-10 | 江苏农林职业技术学院 | Method for enabling zoysia japonica to turn green earlier |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113079998A (en) * | 2021-05-08 | 2021-07-09 | 四川农业大学 | Method for improving cold resistance of red bud pineapples |
| CN114732016A (en) * | 2022-05-18 | 2022-07-12 | 江苏农林职业技术学院 | Preparation for promoting growth of creeping side buds of zoysia macrosperma in severe saline-alkali soil and using method thereof |
| CN114732016B (en) * | 2022-05-18 | 2023-04-25 | 江苏农林职业技术学院 | Preparation for promoting growth of creeping stem lateral buds of zoysia macrophylla in severe saline-alkali soil and application method of preparation |
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