CN112608967A - Mussel protein extraction method and device - Google Patents
Mussel protein extraction method and device Download PDFInfo
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- CN112608967A CN112608967A CN202110037859.XA CN202110037859A CN112608967A CN 112608967 A CN112608967 A CN 112608967A CN 202110037859 A CN202110037859 A CN 202110037859A CN 112608967 A CN112608967 A CN 112608967A
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
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- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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Abstract
The invention relates to the relevant field of deep processing technology of mussel protein, in particular to a mussel protein extraction method and a device, which comprises a mounting rack, a crushing box, a processing box, a centrifuge, a filter and a drying box, wherein the collecting box corresponds to a connecting channel, the top of the connecting channel is communicated with a crushing cavity, the cross section of a filter screen is in an obtuse triangle shape, and two sides of the filter screen are communicated with the connecting channel and are parallel to the top of the collecting box, so that larger mussel meat is conveniently conveyed into the collecting box and then conveyed into the crushing cavity again for continuous crushing, and the quality of the mussel meat entering the processing box is ensured; through the effect of extract and protease, separate by centrifuge and filter again to desalt and concentrate liquid material, obtain mussel protein, rather than the purification of the method of traditional chromatogram, overcome the problem that chromatographic method reaction generates mussel protein equipment and material cost are high, simplified technology, reduced the cost that mussel protein drawed.
Description
Technical Field
The invention relates to the field related to the deep processing technology of mussel protein, in particular to a method and a device for extracting mussel protein.
Background
Mussels, also called Qingkou, are popular seafood products. Can be steamed and cooked, also can be mixed with other green vegetables after being peeled off, and has delicious taste. Due to the large yield of mussels, the mussels are not easy to store after harvesting, and are cooked thoroughly before the time comes, and then the mussels are processed into processed products. Mussel protein powder on the existing market is popular with consumers.
Cutting, grinding are the indispensable link in the mussel albumen powder processing link in the extraction process of mussel protein, but current processing equipment can't satisfy a large amount of production demands, has a great deal of problems in the course of working. For example, current mussel needs prewashing after the shell is removed, processes again and smashes, still can not carry out abundant crushing to mussel meat, and great mussel meat gets into processing equipment together, often can influence the quality of working a project. In addition, the conventional mussel mucin is generally purified by industrial chromatography to obtain a single protein with high purity, but the currently used chromatographic purification method has the problems of expensive equipment materials and high cost.
Disclosure of Invention
The invention aims to provide a mussel protein extraction method and device to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a mussel protein extraction method and device are provided, the method comprises the following steps:
step S1: selecting fresh mussels, removing shells, and crushing the mussels into minced meat serving as a raw material by using a crushing box;
step S2: conveying the processed raw materials into a processing box through a communicating pipe, adding an extracting agent with the mass 3-10 times that of the raw materials, adding a protease reagent with the mass 0.01-0.02 time that of the raw materials and 20U/mg, simultaneously, driving a stirring shaft by a motor to stir for 3.5-4.5h to obtain a mixed solution, starting a first heater after enzymolysis is finished, heating the temperature to 90-95 ℃ by a heating pipe, and inactivating protease for 10-15 min;
step S3: conveying the mixed solution obtained in the step S2 into a centrifugal machine through a liquid pump, and centrifuging the mixed solution for 30-50min to remove fat and insoluble substances to obtain an enzymatic hydrolysate;
step S4: filtering and concentrating the obtained enzymolysis liquid through a filter, and removing salt impurities in the enzymolysis liquid to obtain concentrated mussel protein liquid;
step S5: carry into the drying cabinet with mussel protein liquid, open the second heater and dry to mussel protein liquid and get rid of moisture, obtain mussel protein promptly.
The device comprises a mounting frame, a crushing box, a processing box, a centrifugal machine, a filter and a drying box, wherein the top of the mounting frame is connected with a top plate, the crushing box is installed on one side of the top plate, a crushing cavity is arranged in the middle inside the crushing box, two crushing rollers are installed inside the crushing cavity, a filter screen is installed at the bottom of the crushing cavity, vibrators are connected inside the top plate at two ends of the bottom of the filter screen, connecting channels are arranged at two sides of the crushing cavity, the bottom of each connecting channel is connected with a collecting box in an inner cavity of the top plate in an embedded mode, a communicating pipe is connected at the bottom of the crushing box, the bottom of each communicating pipe is connected with the processing box on the side wall of the inner cavity of the mounting frame, a stirring shaft is installed, handle and install temperature sensor and level sensor on the incasement lateral wall, handle incasement chamber bottom and install the heating pipe, it is connected with first heater to handle the bottom of the case portion, be provided with the baffle in the middle of the mounting bracket inner chamber, install the liquid pump in the middle of the mounting bracket inner chamber bottom, centrifuge is installed to roof top opposite side, be provided with the filter in the mounting bracket inside under centrifuge, the filter bottom is connected with the fixed plate between mounting bracket inner chamber wall and baffle, install the drying cabinet in mounting bracket inner chamber bottom under the fixed plate, drying cabinet inner chamber bottom is provided with the second heater.
Preferably, the extraction solution used in step S2 is 0.5-2.5% perchloric acid and 1-10% acetic acid as extraction solvents.
Preferably, a temperature sensor is installed on the side wall of the inner cavity of the drying oven, heat-preservation sponge is filled in the inner wall of the drying oven, the heating drying oven is heated to 50-55 ℃ by the second heater in the step S5 and is kept for 2-3h, the quality of the mussel protein is guaranteed, meanwhile, the mussel protein is dried more quickly, and the production efficiency is improved.
Preferably, both sides fixedly connected with mounting panel communicating pipe, the telescopic link is installed at the mounting panel top, the telescopic link top is connected through the pivot with collecting the box, it is corresponding with interface channel to collect the box, and the interface channel top is circular-arc and smashes the chamber and be linked together, it is circular-arc to collect box top and bottom edge, is convenient for carry the great mussel meat of granule again and continues to smash in the crushing intracavity, guarantees to get into the quality of the mussel meat of handling the incasement.
Preferably, the cross section of the filter screen is an obtuse triangle, the two sides of the filter screen are communicated with the connecting channel and are parallel and level with the top of the collecting box, and large mussel meat is conveniently conveyed into the collecting box.
Preferably, the number of the reagent boxes is two, an extracting agent is filled in one reagent box, a protease reagent is filled in the other reagent box, the reagent boxes are communicated with the top of the treatment box through connecting pipes, and electromagnetic valves are arranged on the connecting pipes.
Preferably, the liquid pump communicates the bottom of the treatment tank with the centrifuge through a delivery pipe, and the filter communicates with the centrifuge and the drying tank through a delivery pipe.
Preferably, a filter membrane with the pore diameter of 45 μm is adopted in the filter.
Compared with the prior art, the invention has the beneficial effects that: according to the invention, the connecting channel is arranged in the crushing box, the collecting box is arranged at the bottom of the connecting channel, the top of the telescopic rod is connected with the collecting box through the rotating shaft, the collecting box corresponds to the connecting channel, the top of the connecting channel is arc-shaped and is communicated with the crushing cavity, the edges of the top and the bottom of the collecting box are arc-shaped, the cross section of the filter screen is obtuse triangle, the two sides of the filter screen are communicated with the connecting channel and are parallel and level to the top of the collecting box, so that large mussel meat can be conveniently conveyed into the collecting box and then conveyed into the crushing cavity again for continuous crushing, and the quality of the mussel meat entering the processing box is ensured; through the effect of extract and protease, separate by centrifuge and filter again to desalt and concentrate liquid material, obtain mussel protein, rather than the purification of the method of traditional chromatogram, overcome the problem that chromatographic method reaction generates mussel protein equipment and material cost are high, simplified technology, reduced the cost that mussel protein drawed.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
fig. 2 is a schematic structural diagram of the collecting box of the invention.
In the figure: 1. a mounting frame; 2. a crushing box; 3. a treatment tank; 4. a centrifuge; 5. a filter; 6. a drying oven; 7. a top plate; 8. a grinding chamber; 9. a crushing roller; 10. a filter screen; 11. a connecting channel; 12. a collection box; 13. a vibrator; 14. a communicating pipe; 15. a telescopic rod; 16. mounting a plate; 17. a kit; 18. a connecting pipe; 19. an electromagnetic valve; 20. a stirring shaft; 21. a motor; 22. a temperature sensor; 23. a liquid level sensor; 24. heating a tube; 25. a first heater; 26. a partition plate; 27. a liquid pump; 28. a delivery pipe; 29. a transfer tube; 30. a fixing plate; 31. a rotating shaft; 32. a second heater.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "vertical", "upper", "lower", "horizontal", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Referring to fig. 1-2, the present invention provides a technical solution: a mussel protein extraction method and device are provided, the method comprises the following steps:
step S1: selecting fresh mussels, removing shells, and crushing the mussels into minced meat serving as a raw material by using a crushing box 2;
step S2: conveying the processed raw materials into a processing box 3 through a communicating pipe 14, adding an extracting agent with the mass 3-10 times that of the raw materials, adding a protease reagent with the mass 0.01-0.02 time that of the raw materials and 20U/mg, simultaneously, driving a stirring shaft 20 by a motor 21 to stir for 3.5-4.5h to obtain a mixed solution, starting a first heater 25 after enzymolysis is finished, heating the temperature to 90-95 ℃ by a heating pipe 24, and inactivating protease for 10-15 min;
step S3: conveying the mixed liquid in the step S2 into a centrifuge 4 through a liquid pump 27, centrifuging the mixed liquid for 30-50min, and removing fat and insoluble substances to obtain an enzymolysis liquid;
step S4: filtering and concentrating the obtained enzymolysis liquid by a filter 5 to remove salt impurities in the enzymolysis liquid to obtain concentrated mussel protein liquid;
step S5: carry into drying cabinet 6 with mussel protein liquid, open second heater 32 and dry to mussel protein liquid and get rid of moisture, obtain mussel protein promptly.
The device comprises a mounting frame 1, a crushing box 2, a processing box 3, a centrifuge 4, a filter 5 and a drying box 6, wherein the top of the mounting frame 1 is connected with a top plate 7, one side of the top plate 7 is provided with the crushing box 2, the middle inside the crushing box 2 is provided with a crushing cavity 8, the inside of the crushing cavity 8 is provided with two crushing rollers 9, the bottom of the crushing cavity 8 is provided with a filter screen 10, two ends of the bottom of the filter screen 10 are connected with vibrators 13 inside the top plate 7, two sides of the crushing cavity 8 are provided with connecting channels 11, the bottom of the connecting channels 11 is embedded and connected with a collecting box 12 in the inner cavity of the top plate 7, the bottom of the crushing box 2 is connected with a communicating pipe 14, the bottom end of the communicating pipe 14 is connected with the processing box 3 on the side wall of the inner cavity of, install temperature sensor 22 and level sensor 23 on handling 3 inside walls of case, handle 3 inner chambers of case bottom and install heating pipe 24, it is connected with first heater 25 to handle 3 bottoms of case, be provided with baffle 26 in the middle of the 1 inner chamber of mounting bracket, install liquid pump 27 in the middle of the 1 inner chamber bottom of mounting bracket, centrifuge 4 is installed to 7 top opposite sides of roof, centrifuge 4 is under in 1 inside filter 5 that is provided with of mounting bracket, filter 5 bottom is connected with fixed plate 30 between 1 inner chamber wall of mounting bracket and baffle 26, install drying cabinet 6 in 1 inner chamber bottom of mounting bracket under the fixed plate 30, 6 inner chambers of drying cabinet bottom are provided with second heater 32.
Further, the extract used in step S2 is 0.5-2.5% perchloric acid and 1-10% acetic acid as extraction solvents.
Further, a temperature sensor 22 is installed on the inner cavity side wall of the drying oven 6, heat preservation sponge is filled in the inner wall of the drying oven 6, the second heater 32 heats the heating drying oven 6 to 50-55 ℃ in step S5, the temperature is kept for 2-3h, the quality of the mussel protein is guaranteed, meanwhile, the mussel protein is dried more quickly, and the production efficiency is improved.
Further, 14 both sides fixedly connected with mounting panel 16 communicating pipe, telescopic link 15 is installed at mounting panel 16 top, 15 tops of telescopic link are connected through pivot 31 with collection box 12, it is corresponding with interface channel 11 to collect box 12, and interface channel 11 tops is circular-arc and is linked together with smashing the chamber 8, it is circular-arc to collect box 12 tops and bottom edge, be convenient for carry the great mussel meat of granule again and continue smashing in smashing the chamber 8, guarantee to get into the quality of the mussel meat in the processing case 3.
Further, the cross section of the filter screen 10 is an obtuse triangle, two sides of the filter screen 10 are communicated with the connecting channel 11 and are flush with the top of the collecting box 12, so that large mussel meat can be conveyed into the collecting box 12 conveniently
Furthermore, two reagent boxes 17 are arranged, an extracting agent is filled in one reagent box 17, a protease reagent is filled in the other reagent box 17, the reagent boxes 17 are communicated with the top of the treatment box 3 through connecting pipes 18, and electromagnetic valves 19 are arranged on the connecting pipes 18.
Further, a liquid pump 27 communicates the bottom of the treatment tank 3 with the centrifuge 4 through a delivery pipe 28, and the filter 5 communicates with the centrifuge 4 and the drying tank 6 through a delivery pipe 29.
Furthermore, a filter membrane with a pore size of 45 μm is used in the filter 5.
According to the invention, the connecting channel 11 is arranged in the crushing box 2, the collecting box 12 is arranged at the bottom of the connecting channel 11, the top of the telescopic rod 15 is connected with the collecting box 12 through the rotating shaft 31, the collecting box 12 corresponds to the connecting channel 11, the top of the connecting channel 11 is in a circular arc shape and is communicated with the crushing cavity 8, the top and bottom edges of the collecting box 12 are in circular arc shapes, the cross section of the filter screen 10 is in an obtuse triangle shape, two sides of the filter screen 10 are communicated with the connecting channel 11 and are parallel to the top of the collecting box 12, so that larger mussel meat can be conveniently conveyed into the collecting box 12 and then conveyed into the crushing cavity 8 again for continuous crushing, and the quality of the mussel meat entering the processing box is ensured; through the effect of extract and protease, separate by centrifuge 4 and filter 5 to the liquid material that will contain mussel protein carries out desalination and concentration, obtains mussel protein through drying again, and the method of non-traditional chromatogram purifies, overcomes the problem that chromatographic method reaction generates mussel protein equipment and material cost are high, has simplified the technology, has reduced the cost that mussel protein drawed.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. A mussel protein extraction method and device are characterized by comprising the following steps:
step S1: selecting fresh mussels, removing shells, and crushing the mussels into minced meat serving as a raw material by using a crushing box (2);
step S2: conveying the processed raw materials into a processing box (3) through a communicating pipe (14), adding an extracting agent with the mass 3-10 times that of the raw materials, adding a protease reagent with the mass 0.01-0.02 time that of the raw materials and 20U/mg, simultaneously, driving a stirring shaft (20) by a motor (21) to stir for 3.5-4.5h to obtain a mixed solution, starting a first heater (25) after enzymolysis is finished, heating the temperature to 90-95 ℃ by a heating pipe (24), and inactivating protease for 10-15 min;
step S3: conveying the mixed liquid in the step S2 into a centrifuge (4) through a liquid pump (27), centrifuging the mixed liquid for 30-50min, and removing fat and insoluble substances to obtain an enzymolysis liquid;
step S4: filtering and concentrating the obtained enzymolysis liquid by a filter (5), and removing salt impurities in the enzymolysis liquid to obtain concentrated mussel protein liquid;
step S5: the mussel protein liquid is conveyed into the drying box (6), and the second heater (32) is turned on to dry the mussel protein liquid to remove moisture, so that the mussel protein is obtained.
The device comprises a mounting rack (1), a crushing box (2), a processing box (3), a centrifuge (4), a filter (5) and a drying box (6), and is characterized in that: the top of the mounting frame (1) is connected with a top plate (7), one side of the top plate (7) is provided with a crushing box (2), the middle inside the crushing box (2) is provided with a crushing cavity (8), the inside of the crushing cavity (8) is provided with two crushing rollers (9), the bottom of the crushing cavity (8) is provided with a filter screen (10), two ends of the bottom of the filter screen (10) are connected with vibrators (13) inside the top plate (7), two sides of the crushing cavity (8) are provided with connecting channels (11), the bottom of the connecting channels (11) is embedded and connected with a collecting box (12) in the inner cavity of the top plate (7), the bottom of the crushing box (2) is connected with communicating pipes (14), the bottom of the communicating pipes (14) is connected with a processing box (3) on the side wall of the inner cavity of the mounting, (mixing) shaft (20) one end is connected with motor (21) on mounting bracket (1) lateral wall, motor (21) top is installed kit (17) on mounting bracket (1) lateral wall, install temperature sensor (22) and level sensor (23) on handling case (3) inside wall, handle case (3) inner chamber bottom and install heating pipe (24), it is connected with first heater (25) to handle case (3) bottom, be provided with baffle (26) in the middle of mounting bracket (1) inner chamber, install liquid pump (27) in the middle of mounting bracket (1) inner chamber bottom, centrifuge (4) is installed to roof (7) top opposite side, be provided with filter (5) in mounting bracket (1) inside under centrifuge (4), filter (5) bottom is connected with fixed plate (30) between mounting bracket (1) inner chamber wall and baffle (26), drying cabinet (6) is installed to mounting bracket (1) inner chamber bottom under fixed plate (30), drying cabinet (6) inner chamber bottom is provided with second heater (32).
2. The mussel protein extraction method and device according to claim 1, wherein: the extracting agent used in step S2 is 0.5-2.5% perchloric acid and 1-10% acetic acid as extraction solvent.
3. The mussel protein extraction method and device according to claim 1, wherein: a temperature sensor (22) is installed on the side wall of the inner cavity of the drying box (6), heat-preservation sponge is filled in the inner wall of the drying box (6), and the second heater (32) heats the heating drying box (6) to 50-55 ℃ in step S5 and keeps the temperature for 2-3 h.
4. The mussel protein extraction method and device according to claim 1, wherein: communicating pipe (14) both sides fixedly connected with mounting panel (16), telescopic link (15) are installed at mounting panel (16) top, telescopic link (15) top is connected through pivot (31) with collection box (12), it is corresponding with interface channel (11) to collect box (12), and interface channel (11) top is circular-arc and smashes chamber (8) and be linked together, it is circular-arc to collect box (12) top and bottom edge.
5. The mussel protein extraction method and device according to claim 1, wherein: the cross section of the filter screen (10) is an obtuse triangle, and two sides of the filter screen (10) are communicated with the connecting channel (11) and are parallel and level with the top of the collecting box (12).
6. The mussel protein extraction method and device according to claim 1, wherein: the kit (17) is provided with two kits, an extracting agent is filled in one kit (17), a protease reagent is filled in the other kit (17), the kit (17) is communicated with the top of the treatment box (3) through a connecting pipe (18), and an electromagnetic valve (19) is arranged on the connecting pipe (18).
7. The mussel protein extraction method and device according to claim 1, wherein: the liquid pump (27) is used for communicating the bottom of the processing box (3) with the centrifuge (4) through a conveying pipe (28), and the filter (5) is communicated with the centrifuge (4) and the drying box (6) through a conveying pipe (29).
8. The mussel protein extraction method and device according to claim 1, wherein: and a filter membrane with the pore diameter of 45 mu m is adopted in the filter (5).
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113698449A (en) * | 2021-09-27 | 2021-11-26 | 吉林大学 | Processing and extracting equipment and process for protein components in cyperus esculentus |
| CN114958598A (en) * | 2022-05-15 | 2022-08-30 | 哈尔滨商业大学 | Equipment and method for extracting and purifying inonotus obliquus polysaccharide |
| CN115260279A (en) * | 2022-06-23 | 2022-11-01 | 陕西理工大学 | Giant salamander collagen skin and secretion protein peptide extraction element |
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| CN102771621A (en) * | 2011-05-13 | 2012-11-14 | 浙江海洋学院 | Enzymolysis method of mussel protein |
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| CN114958598A (en) * | 2022-05-15 | 2022-08-30 | 哈尔滨商业大学 | Equipment and method for extracting and purifying inonotus obliquus polysaccharide |
| CN115260279A (en) * | 2022-06-23 | 2022-11-01 | 陕西理工大学 | Giant salamander collagen skin and secretion protein peptide extraction element |
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| CN112608967B (en) | 2021-09-03 |
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