CN112608828A - Device and method for detecting activity of lactic acid bacteria - Google Patents

Device and method for detecting activity of lactic acid bacteria Download PDF

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Publication number
CN112608828A
CN112608828A CN202011518963.2A CN202011518963A CN112608828A CN 112608828 A CN112608828 A CN 112608828A CN 202011518963 A CN202011518963 A CN 202011518963A CN 112608828 A CN112608828 A CN 112608828A
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fixedly connected
pressure steam
activity
plate
steam sterilization
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CN112608828B (en
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刘秉杰
杨炳坤
陈志球
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Guangdong Shilong Jinwei Drink & Food Co ltd
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Guangdong Shilong Jinwei Drink & Food Co ltd
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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Abstract

The invention discloses a device for detecting the activity of lactic acid bacteria, which comprises a bottom plate, wherein the upper end of the bottom plate is fixedly connected with a heating box body, a high-pressure steam sterilization chamber and an activity detection chamber, the surface of the upper end of the bottom plate is fixedly connected with a fixed frame, the outer surface of the fixed frame is fixedly connected with a fixed plate, the surface of one side of the fixed plate is fixedly connected with an electric push rod, the output end of the electric push rod is fixedly connected with sliding blocks, and a connecting plate is fixedly connected among the four sliding blocks. The whole extraction pipe can be mechanically moved correspondingly through the fixing frame, the electric push rod and the fixing plate, so that the operation of a user is facilitated, and the whole boiling, high-temperature sterilization and detection are integrated, so that the trouble caused by the operation of parts is avoided, the whole detection can be finished by one person, the labor expenditure is saved, and the use efficiency of the device is improved.

Description

Device and method for detecting activity of lactic acid bacteria
Technical Field
The invention relates to the technical field of activity detection equipment, in particular to a device and a method for detecting the activity of lactic acid bacteria.
Background
Lactic acid bacteria are a general term for a group of bacteria capable of utilizing fermentable carbohydrates to produce a large amount of lactic acid, the bacteria are very widely distributed in the nature, have abundant species diversity, at least comprise 18 genera, more than 200, and most of the bacteria are flora which are indispensable in the human body and have important physiological functions except a very small number, and widely exist in the intestinal tract of the human body.
However, the existing lactobacillus activity detection method has the following defects:
1. the existing lactobacillus activity detection device is mainly subjected to labor division treatment, and needs more hands, so that a large amount of labor expense is brought, moreover, the whole body needs to be manually carried out in one step, and corresponding mechanical treatment is difficult to carry out, so that the whole detection is too complicated and troublesome;
2. wholly when breaking down, hardly carry out corresponding analysis of disassembling, just so very difficult quick discovery problem and the quick maintenance of going on to it is difficult to put into use once more to cause the device, reduces the availability factor of whole device.
3. The existing detection method mostly adopts a microscope to directly count, so that corresponding degreasing treatment is lacked, the number of lactobacillus active strains cannot be correctly reflected to a certain extent, certain influence is brought to the accuracy of results, and the inaccuracy of the results is caused.
Disclosure of Invention
The invention aims to provide a device and a method for detecting the activity of lactic acid bacteria, and aims to solve the problems that most of the conventional lactic acid bacteria activity detection devices are subjected to labor division treatment and require more hands, so that a large amount of labor expenditure is brought, the automation degree is low, and when the whole body fails, corresponding disassembly analysis and detection methods are difficult to perform, and direct counting is mostly carried out by using a microscope, so that corresponding degreasing treatment is lacked, and the number of lactic acid bacteria active strains cannot be correctly reflected to a certain degree.
In order to solve the technical problems, the invention provides the following technical scheme: a device for detecting the activity of lactic acid bacteria comprises a base plate, wherein the upper end of the base plate is fixedly connected with a heating box body, a high-pressure steam sterilization chamber and an activity detection chamber, the upper end of the base plate is fixedly connected with a fixed frame, the outer surface of the fixed frame is fixedly connected with a fixed plate, one side surface of the fixed plate is fixedly connected with an electric push rod, the output end of the electric push rod is fixedly connected with a sliding block, four sliding blocks are fixedly connected with a connecting plate, the upper end of the connecting plate is fixedly provided with a hydraulic cylinder, the output end of the hydraulic cylinder is fixedly connected with a transverse fixed plate, the inner part of the transverse fixed plate is fixedly connected with an extraction pipe, the inner piston of the extraction pipe is connected with a piston plate, the upper end of the piston plate is fixedly connected with a pulling rod, the inner bottom wall of the high-pressure, the high-pressure steam sterilization chamber is characterized in that a liquid inlet is fixedly connected to the surface of the upper end of the high-pressure steam sterilization chamber, a detection head is fixedly connected to the upper end of the activity detection chamber, a display is fixedly mounted at the upper end of the detection head, and a heating arc plate is arranged on the outer surface of the heating box body.
Further, two the surface of heating arc board is first connecting block of fixedly connected with and second connecting block respectively, one side fixed surface of first connecting block is connected with square inserted bar, spacing jack has been seted up to the one end upper surface of square inserted bar, the upper end fixed surface of second connecting block is connected with the L template, the upper end fixed surface of L template is connected with the fretwork piece, the inside of fretwork piece is inserted and is equipped with spacing inserted bar, the upper end fixedly connected with pull block of spacing inserted bar, the outer fixed surface of spacing inserted bar is connected with spacing ring piece, the surface cover of spacing inserted bar is equipped with the spring.
Further, the upper end fixed mounting of bottom plate has the block terminal, the upper end fixed mounting of block terminal has first electrical property to connect, the fixed surface of heating arc board installs the second electrical property and connects, electrically connected with wire between first electrical property and the second electrical property connects, the fixed surface of block terminal is fixed mounting respectively has control switch and display lamp, the positive fixed mounting of high-pressure steam sterilization room has the hinge, high-pressure steam sterilization room has the glass frame through the hinge rotation connection.
Furthermore, one end of the spring is fixedly connected with the surface of the upper end of the limiting ring piece, and the other end of the spring is fixedly connected with the inner top wall of the hollow block.
Furthermore, the lower end of the limiting insertion rod is inserted into the inner wall of the limiting insertion hole, the set inner diameter of the limiting insertion hole is matched with the designed outer diameter of the lower end of the limiting insertion rod, and the limiting ring piece and the spring are both arranged inside the hollow block.
Furthermore, the sliding block is in the surface sliding connection of mount, two the interval between the inlet is unanimous with the interval design between two extraction pipes.
The invention also provides a method for detecting the activity of the lactic acid bacteria, which comprises the following steps:
s1, firstly, introducing milk into the heating box body, then starting the heating arc plate to heat the heating box body until the milk in the heating box body is boiled, then continuing heating and boiling, and then correspondingly cooling overnight to enable the fat to float;
s2, starting the electric push rod to enable the electric push rod to push the sliding block to move on the outer surface of the fixing frame, stopping the electric push rod when the connecting plate moves right above the heating box body, then starting the hydraulic cylinder to enable the hydraulic cylinder to push the transverse fixing plate to descend, then pulling the piston plate through the pulling rod to enable the extracting pipe to suck out bottom milk, and abandoning upper-layer milk fat;
s3, respectively starting the hydraulic cylinder and the electric push rod again, enabling the connecting plate to be located right above the high-pressure steam sterilization chamber, enabling the lower end of the extracting pipe to be aligned to the liquid inlet, then pushing the pulling rod, enabling the piston plate to extrude the emulsion inside, then dripping the emulsion into the container through the liquid inlet, and then starting the high-pressure steam sterilization chamber to perform high-pressure steam sterilization on the emulsion inside the container;
s4, taking out the emulsion sterilized by high-pressure steam with the container, and then putting the emulsion into an activity detection chamber, so as to perform corresponding detection under the action of the detection head, and finally obtaining the detection result through a display.
Further, the boiling time in the S1 is controlled to be 20-30min, the temperature in the high-pressure steam sterilization chamber in the S3 is controlled to be 115-130 ℃, and the high-pressure steam sterilization time is controlled to be 30-40 min.
Compared with the prior art, the invention has the following beneficial effects:
(1) the whole extraction pipe can be mechanically moved correspondingly through the fixing frame, the electric push rod and the fixing plate, so that the operation of a user is facilitated, and the whole boiling, high-temperature sterilization and detection are integrated, so that the trouble caused by the operation of parts is avoided, the whole detection can be finished by one person, the labor expenditure is saved, and the use efficiency of the device is improved.
(2) According to the invention, the heating arc plate can be rapidly disassembled and maintained when being damaged through the square inserting rod, the limiting inserting hole and the limiting inserting rod, so that the maintenance of equipment can be more convenient and simpler, and moreover, through the transparent glass designed in the glass frame, a user can know the internal condition of the high-pressure steam sterilization chamber at the first time, so that the fault can be conveniently and rapidly known where the fault occurs, the maintenance work can be rapidly carried out, and the device can be put into use again at the first time.
(3) According to the invention, a method of degreasing, high-pressure steam sterilization and detection is adopted, so that the number of lactobacillus active strains can be reflected better, the detection of lactobacillus activity is more accurate, and the influence of inaccurate detection on the later period is avoided.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a front view of the structure of the present invention;
FIG. 3 is a top view of the structure of the present invention;
FIG. 4 is an enlarged view of the structure of FIG. 1 at A in accordance with the present invention;
FIG. 5 is a front cross-sectional view of a partial connection structure of the hydraulic cylinder and the extraction pipe of the present invention;
fig. 6 is a schematic view of a partial connection structure of a second heating arc plate and a distribution box according to the present invention.
In the figure: 1. a base plate; 2. heating the box body; 3. a high-pressure steam sterilization chamber; 4. an activity detection chamber; 5. a fixed mount; 6. a fixing plate; 7. an electric push rod; 8. a slider; 9. a connecting plate; 10. a hydraulic cylinder; 11. transversely fixing the plate; 12. an extraction pipe; 13. a piston plate; 14. pulling a rod; 15. a bearing table; 16. a holding container; 17. a liquid inlet; 18. a detection head; 19. a display; 20. heating the arc plate; 21. a first connection block; 22. a square inserted link; 23. a second connecting block; 24. limiting the jacks; 25. an L-shaped plate; 26. hollowing out the block; 27. a limiting inserted rod; 28. pulling the block; 29. a limit ring sheet; 30. a spring; 31. a distribution box; 32. a first electrical connector; 33. a second electrical connector; 34. a wire; 35. a control switch; 36. a display lamp; 37. a hinge; 38. a glass frame.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-6, the present invention provides the following technical solutions: a device for detecting the activity of lactic acid bacteria comprises a base plate 1, wherein the upper end of the base plate 1 is fixedly connected with a heating box body 2, a high-pressure steam sterilization chamber 3 and an activity detection chamber 4, the upper end of the base plate 1 is fixedly connected with a fixed frame 5, the outer surface of the fixed frame 5 is fixedly connected with a fixed plate 6, one side surface of the fixed plate 6 is fixedly connected with an electric push rod 7, the output end of the electric push rod 7 is fixedly connected with a sliding block 8, a connecting plate 9 is fixedly connected among the four sliding blocks 8, the upper end of the connecting plate 9 is fixedly provided with a hydraulic cylinder 10, the output end of the hydraulic cylinder 10 is fixedly connected with a transverse fixed plate 11, the inner part of the transverse fixed plate 11 is fixedly connected with an extraction pipe 12, the inner piston of the extraction pipe 12 is connected with a piston plate 13, the upper end of, the upper end surface of plummer 15 has been placed and has been held container 16, and the upper end fixed surface of high-pressure steam sterilization room 3 is connected with inlet 17, and the activity detects the upper end fixedly connected with of room 4 and detects head 18, and the upper end fixed mounting who detects head 18 has display 19, and the surface of heating box 2 is provided with heating arc board 20.
Through setting degrease, pasteurization and detection to an organic whole, it is better to let holistic operation use the manpower to it is not high to measurement personnel's technical requirement, avoid the instrument to need the staff problem inadequately that professional operation leads to.
Specifically, the surface of two heating arc boards 20 is fixedly connected with first connecting block 21 and second connecting block 23 respectively, a side fixed surface of first connecting block 21 is connected with square inserted bar 22, spacing jack 24 has been seted up to the one end upper surface of square inserted bar 22, the upper end fixed surface of second connecting block 23 is connected with L template 25, the upper end fixed surface of L template 25 is connected with fretwork piece 26, the inside of fretwork piece 26 is inserted and is equipped with spacing inserted bar 27, the upper end fixedly connected with pulling block 28 of spacing inserted bar 27, the outer fixed surface of spacing inserted bar 27 is connected with spacing ring piece 29, the surface cover of spacing inserted bar 27 is equipped with spring 30.
Through setting up square inserted bar 22, spacing inserted bar 27 and spacing jack 24 for two heating arc boards 20 can carry out quick dismouting, thereby made things convenient for the maintenance work in later stage, let continuation use that whole equipment can be better.
Specifically, the upper end fixed mounting of bottom plate 1 has block terminal 31, the upper end fixed mounting of block terminal 31 has first electrical property joint 32, the fixed surface of heating arc board 20 installs second electrical property joint 33, electrical connection has wire 34 between first electrical property joint 32 and the second electrical property joint 33, the fixed surface of block terminal 31 has control switch 35 and display lamp 36 respectively fixed mounting, the positive fixed mounting of high-pressure steam sterilization room 3 has hinge 37, high-pressure steam sterilization room 3 rotates through hinge 37 and is connected with glass frame 38.
By designing the glass frame 38, the glass frame 38 is matched with transparent glass, so that an operator can better observe the condition inside the high-pressure steam sterilization chamber 3, and the later-stage rapid treatment is facilitated.
Specifically, one end of the spring 30 is fixedly connected with the upper end surface of the limit ring piece 29, and the other end of the spring 30 is fixedly connected with the inner top wall of the hollow block 26.
The two ends of the spring 30 are fixedly connected with the upper end surface of the limit ring sheet 29 and the inner top wall of the hollow block 26 respectively, so that the spring 30 can achieve corresponding buffering capacity while achieving connectivity.
Specifically, the lower end of the limiting insertion rod 27 is inserted into the inner wall of the limiting insertion hole 24, the opening inner diameter of the limiting insertion hole 24 is matched with the design outer diameter of the lower end of the limiting insertion rod 27, and the limiting ring piece 29 and the spring 30 are both arranged inside the hollow block 26.
Through the inner diameter design with spacing jack 24 unanimous with the outer diameter of spacing inserted bar 27 lower extreme to let the lower extreme of spacing inserted bar 27 insert and establish can be more after the inner wall of spacing jack 24 with stable adaptation.
Specifically, the sliding block 8 is connected to the outer surface of the fixing frame 5 in a sliding manner, and the distance between the two liquid inlets 17 is designed to be consistent with the distance between the two extraction pipes 12.
Through the design of the space between the two liquid inlets 17 and the space between the two extraction pipes 12, the liquid in the later extraction pipe 12 can better fall into the containing container (16) through the liquid inlets 17.
The invention also provides a method for detecting the activity of the lactic acid bacteria, which comprises the following steps:
s1, firstly, introducing milk into the heating box body 2, then starting the heating arc plate 20 to heat the heating box body 2 until the milk in the heating box body is boiled, then continuing heating and boiling, and then correspondingly cooling overnight to enable the fat to float;
s2, starting the electric push rod 7, enabling the electric push rod 7 to push the sliding block 8 to move on the outer surface of the fixed frame 5, stopping the electric push rod 7 when the connecting plate 9 moves right above the heating box body 2, then starting the hydraulic cylinder 10, enabling the hydraulic cylinder 10 to push the transverse fixed plate 11 to descend, then pulling the piston plate 13 through the pulling rod 14, enabling the extraction pipe 12 to suck out bottom milk, and abandoning upper-layer milk fat;
s3, respectively starting the hydraulic cylinder 10 and the electric push rod 7 again, enabling the connecting plate 9 to be located right above the high-pressure steam sterilization chamber 3, enabling the lower end of the extracting pipe 12 to be aligned to the liquid inlet 17, then pushing the pulling rod 14, enabling the piston plate 13 to extrude the emulsion inside, then dropping the emulsion into the containing container 16 through the liquid inlet 17, and then starting the high-pressure steam sterilization chamber 3 to perform high-pressure steam sterilization on the emulsion inside the containing container 16;
s4, taking out the emulsion sterilized by high-pressure steam together with the holding container 16, then putting the emulsion into the activity detection chamber 4, carrying out corresponding detection under the action of the detection head 18, and finally obtaining the detection result through the display 19.
Specifically, the boiling time in S1 is controlled to be 20-30min, the temperature in the high-pressure steam sterilization chamber 3 in S3 is controlled to be 115-130 ℃, and the high-pressure steam sterilization time is controlled to be 30-40 min.
The boiling time is effectively controlled, so that the whole can be better degreased in the later period, and the temperature in the high-pressure steam sterilization chamber 3 is controlled at 115-130 ℃, so that a better sterilization effect can be obtained.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (8)

1. An apparatus for detecting activity of lactic acid bacteria, characterized in that: comprises a bottom plate (1), an upper end subsection of the bottom plate (1) is fixedly connected with a heating box body (2), a high-pressure steam sterilization chamber (3) and an activity detection chamber (4), the upper end surface of the bottom plate (1) is fixedly connected with a fixing frame (5), the outer surface of the fixing frame (5) is fixedly connected with a fixing plate (6), one side surface of the fixing plate (6) is fixedly connected with an electric push rod (7), the output end of the electric push rod (7) is fixedly connected with a sliding block (8), four sliding blocks (8) are fixedly connected with a connecting plate (9), the upper end of the connecting plate (9) is fixedly provided with a hydraulic cylinder (10), the output end of the hydraulic cylinder (10) is fixedly connected with a transverse fixing plate (11), the inner part of the transverse fixing plate (11) is fixedly connected with an extraction pipe (12), and the inner piston of the extraction, the upper end fixed surface of piston plate (13) is connected with pulling rod (14), interior diapire fixedly connected with plummer (15) of high pressure steam sterilization room (3), the upper end surface of plummer (15) has been placed and has been held container (16), the upper end fixed surface of high pressure steam sterilization room (3) is connected with inlet (17), the activity detects upper end fixedly connected with detection head (18) of room (4), the upper end fixed mounting who detects head (18) has display (19), the surface of heating box (2) is provided with heating arc board (20).
2. The apparatus for detecting the activity of lactic acid bacteria according to claim 1, wherein: two the surperficial difference fixedly connected with first connecting block (21) and second connecting block (23) of heating arc board (20), one side fixed surface of first connecting block (21) is connected with square inserted bar (22), spacing jack (24) have been seted up to the one end upper surface of square inserted bar (22), the upper end fixed surface of second connecting block (23) is connected with L template (25), the upper end fixed surface of L template (25) is connected with fretwork piece (26), the inside of fretwork piece (26) is inserted and is equipped with spacing inserted bar (27), the upper end fixedly connected with of spacing inserted bar (27) pulls piece (28), the outer fixed surface of spacing inserted bar (27) is connected with spacing ring piece (29), the outer surface cover of spacing inserted bar (27) is equipped with spring (30).
3. The apparatus for detecting the activity of lactic acid bacteria according to claim 1, wherein: the utility model discloses a high-pressure steam sterilization room, including bottom plate (1), upper end fixed mounting of bottom plate (1) has block terminal (31), the upper end fixed mounting of block terminal (31) has first electric property to connect (32), the fixed surface of heating arc board (20) installs second electric property to connect (33), electric connection has wire (34) between first electric property to connect (32) and second electric property to connect (33), the fixed surface of block terminal (31) is fixed mounting respectively has control switch (35) and display lamp (36), the positive fixed mounting of high-pressure steam sterilization room (3) has hinge (37), high-pressure steam sterilization room (3) are rotated through hinge (37) and are connected with glass frame (38).
4. The apparatus for detecting the activity of lactic acid bacteria according to claim 2, wherein: one end of the spring (30) is fixedly connected with the upper end surface of the limit ring piece (29), and the other end of the spring (30) is fixedly connected with the inner top wall of the hollow block (26).
5. The apparatus for detecting the activity of lactic acid bacteria according to claim 2, wherein: the lower end of the limiting insertion rod (27) is inserted into the inner wall of the limiting insertion hole (24), the opening inner diameter of the limiting insertion hole (24) is matched with the design outer diameter of the lower end of the limiting insertion rod (27), and the limiting ring piece (29) and the spring (30) are arranged inside the hollow block (26).
6. The apparatus for detecting the activity of lactic acid bacteria according to claim 1, wherein: the sliding block (8) is in sliding connection with the outer surface of the fixing frame (5), and the distance between the two liquid inlets (17) is designed to be consistent with the distance between the two extraction pipes (12).
7. The method for detecting the activity of lactic acid bacteria according to any one of claims 1 to 6, comprising the steps of:
s1, firstly, introducing milk into the heating box body (2), then starting the heating arc plate (20) to heat the heating box body (2) until the milk in the heating box body is boiled, then continuing heating and boiling, and then correspondingly cooling overnight to enable the fat to float;
s2, starting the electric push rod (7), enabling the electric push rod (7) to push the sliding block (8) to move on the outer surface of the fixing frame (5), stopping the electric push rod (7) when the connecting plate (9) moves right above the heating box body (2), then starting the hydraulic cylinder (10), enabling the hydraulic cylinder (10) to push the transverse fixing plate (11) to descend, then pulling the piston plate (13) through the pulling rod (14), enabling the extraction pipe (12) to suck out bottom milk, and abandoning upper-layer milk fat;
s3, respectively starting the hydraulic cylinder (10) and the electric push rod (7) again, enabling the connecting plate (9) to be located right above the high-pressure steam sterilization chamber (3), enabling the lower end of the extracting pipe (12) to be aligned to the liquid inlet (17), then pushing the pulling rod (14), further enabling the piston plate (13) to extrude the emulsion inside, then dropping the emulsion into the containing container (16) through the liquid inlet (17), and then starting the high-pressure steam sterilization chamber (3) to perform high-pressure steam sterilization on the emulsion inside the containing container (16);
s4, taking out the emulsion sterilized by high-pressure steam together with the holding container (16), then putting the emulsion into the activity detection chamber (4), carrying out corresponding detection under the action of the detection head (18), and finally obtaining the detection result through the display (19).
8. The method for detecting the activity of lactic acid bacteria according to claim 7, wherein: the boiling time in the S1 is controlled to be 20-30min, the temperature in the high-pressure steam sterilization chamber (3) in the S3 is controlled to be 115-130 ℃, and the high-pressure steam sterilization time is controlled to be 30-40 min.
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