CN112602876B - Nanoemulsion based on natural bacteriostat eutectic solvent and preparation and application thereof - Google Patents
Nanoemulsion based on natural bacteriostat eutectic solvent and preparation and application thereof Download PDFInfo
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- 230000005496 eutectics Effects 0.000 title claims abstract description 65
- 239000002904 solvent Substances 0.000 title claims abstract description 63
- 239000007908 nanoemulsion Substances 0.000 title claims abstract description 61
- 238000002360 preparation method Methods 0.000 title abstract description 12
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 30
- 235000014113 dietary fatty acids Nutrition 0.000 claims abstract description 23
- 229930195729 fatty acid Natural products 0.000 claims abstract description 23
- 239000000194 fatty acid Substances 0.000 claims abstract description 23
- 150000004665 fatty acids Chemical class 0.000 claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- NOOLISFMXDJSKH-UTLUCORTSA-N (+)-Neomenthol Chemical compound CC(C)[C@@H]1CC[C@@H](C)C[C@@H]1O NOOLISFMXDJSKH-UTLUCORTSA-N 0.000 claims abstract description 13
- NOOLISFMXDJSKH-UHFFFAOYSA-N DL-menthol Natural products CC(C)C1CCC(C)CC1O NOOLISFMXDJSKH-UHFFFAOYSA-N 0.000 claims abstract description 13
- 229940041616 menthol Drugs 0.000 claims abstract description 13
- 150000003505 terpenes Chemical class 0.000 claims abstract description 13
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 12
- 235000013305 food Nutrition 0.000 claims abstract description 11
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims abstract description 11
- 229920000053 polysorbate 80 Polymers 0.000 claims abstract description 11
- RECUKUPTGUEGMW-UHFFFAOYSA-N carvacrol Chemical compound CC(C)C1=CC=C(C)C(O)=C1 RECUKUPTGUEGMW-UHFFFAOYSA-N 0.000 claims abstract description 3
- HHTWOMMSBMNRKP-UHFFFAOYSA-N carvacrol Natural products CC(=C)C1=CC=C(C)C(O)=C1 HHTWOMMSBMNRKP-UHFFFAOYSA-N 0.000 claims abstract description 3
- 235000007746 carvacrol Nutrition 0.000 claims abstract description 3
- WYXXLXHHWYNKJF-UHFFFAOYSA-N isocarvacrol Natural products CC(C)C1=CC=C(O)C(C)=C1 WYXXLXHHWYNKJF-UHFFFAOYSA-N 0.000 claims abstract description 3
- POULHZVOKOAJMA-UHFFFAOYSA-N dodecanoic acid Chemical compound CCCCCCCCCCCC(O)=O POULHZVOKOAJMA-UHFFFAOYSA-N 0.000 claims description 25
- 239000000839 emulsion Substances 0.000 claims description 21
- 239000002245 particle Substances 0.000 claims description 20
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 claims description 17
- GHVNFZFCNZKVNT-UHFFFAOYSA-N decanoic acid Chemical compound CCCCCCCCCC(O)=O GHVNFZFCNZKVNT-UHFFFAOYSA-N 0.000 claims description 16
- 239000005639 Lauric acid Substances 0.000 claims description 11
- GYSCBCSGKXNZRH-UHFFFAOYSA-N 1-benzothiophene-2-carboxamide Chemical group C1=CC=C2SC(C(=O)N)=CC2=C1 GYSCBCSGKXNZRH-UHFFFAOYSA-N 0.000 claims description 10
- 238000000265 homogenisation Methods 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 8
- 229960002446 octanoic acid Drugs 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 6
- BWPGKXYWPBQBPV-ZOADXXHESA-N Theasaponin Natural products O=C(O[C@@H]1[C@@H](OC(=O)C)[C@]2(CO)[C@@H](O)C[C@@]3(C)[C@@]4(C)[C@@H]([C@]5(C)[C@H]([C@@](CO)(C)[C@@H](O[C@@H]6[C@@H](O[C@@H]7[C@H](O[C@@H]8[C@@H](O)[C@H](O)[C@H](O)CO8)[C@H](O)[C@@H](O)CO7)[C@@H](O[C@H]7[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O7)[C@H](O)[C@@H](C(=O)O)O6)CC5)CC4)CC=C3[C@@H]2CC1(C)C)/C(=C/C)/C BWPGKXYWPBQBPV-ZOADXXHESA-N 0.000 claims description 5
- BWPGKXYWPBQBPV-MWQJAWBESA-N Theasaponin Chemical compound O([C@H]1[C@H](O)[C@H](O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@@H](O)CO1)O[C@H]1[C@@H]([C@@H](O)[C@H](O)CO1)O)O[C@H]1CC[C@]2(C)[C@H]3CC=C4[C@@]([C@@]3(CC[C@H]2[C@@]1(CO)C)C)(C)C[C@@H](O)[C@@]1(CO)[C@@H](OC(C)=O)[C@@H](C(C[C@H]14)(C)C)OC(=O)C(\C)=C/C)C(O)=O)[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BWPGKXYWPBQBPV-MWQJAWBESA-N 0.000 claims description 5
- OBETXYAYXDNJHR-UHFFFAOYSA-N alpha-ethylcaproic acid Natural products CCCCC(CC)C(O)=O OBETXYAYXDNJHR-UHFFFAOYSA-N 0.000 claims description 5
- 238000010008 shearing Methods 0.000 claims description 5
- 239000005632 Capric acid (CAS 334-48-5) Substances 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 239000005635 Caprylic acid (CAS 124-07-2) Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 238000002604 ultrasonography Methods 0.000 claims 2
- MGSRCZKZVOBKFT-UHFFFAOYSA-N thymol Chemical compound CC(C)C1=CC=C(C)C=C1O MGSRCZKZVOBKFT-UHFFFAOYSA-N 0.000 abstract description 14
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 9
- 239000005844 Thymol Substances 0.000 abstract description 8
- 229960000790 thymol Drugs 0.000 abstract description 8
- 241001122767 Theaceae Species 0.000 abstract description 3
- 239000001397 quillaja saponaria molina bark Substances 0.000 abstract description 3
- 229930182490 saponin Natural products 0.000 abstract description 3
- 150000007949 saponins Chemical class 0.000 abstract description 3
- 235000007586 terpenes Nutrition 0.000 abstract description 3
- 108010046377 Whey Proteins Proteins 0.000 abstract description 2
- 102000007544 Whey Proteins Human genes 0.000 abstract description 2
- 235000021119 whey protein Nutrition 0.000 abstract description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 7
- 241000588724 Escherichia coli Species 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 239000000022 bacteriostatic agent Substances 0.000 description 4
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 230000000845 anti-microbial effect Effects 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000002195 synergetic effect Effects 0.000 description 3
- 239000000341 volatile oil Substances 0.000 description 3
- 235000021472 generally recognized as safe Nutrition 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 208000019331 Foodborne disease Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000003975 animal breeding Methods 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- -1 capric acid-menthol Chemical compound 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 244000078673 foodborn pathogen Species 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 239000010773 plant oil Substances 0.000 description 1
- 239000003495 polar organic solvent Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3481—Organic compounds containing oxygen
- A23L3/3508—Organic compounds containing oxygen containing carboxyl groups
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3481—Organic compounds containing oxygen
- A23L3/349—Organic compounds containing oxygen with singly-bound oxygen
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
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Abstract
The invention discloses a nanoemulsion based on a natural bacteriostat eutectic solvent, and preparation and application thereof, wherein the nanoemulsion comprises the following components in percentage by volume: 10-20% of hydrophobic eutectic solvent, 77-85% of water and 3-5% of emulsifying agent; wherein the hydrophobic eutectic solvent comprises: the molar ratio is 1: 2-3 of two kinds of C 8 ～C 12 Linear fatty acids, and the chain length of the linear fatty acids with large molar quantity is shorter than that of the linear fatty acids with small molar quantity; or, the molar ratio is 1:1 to 3C 8 ～C 12 Linear fatty acids and terpenoids, or in a molar ratio of 1: 1-3 of two terpenoids; the terpenoid is selected from thymol, menthol or carvacrol; the emulsifier is selected from Tween 80, tea saponin or whey protein. The nano emulsion has a certain antibacterial effect, is environment-friendly, can be conveyed in a water phase, exerts the antibacterial effect of fatty acid or terpenes, and can be applied to the food industry.
Description
Technical Field
The invention relates to a stable nanoemulsion, in particular to a nanoemulsion based on a natural bacteriostatic agent eutectic solvent, and preparation and application thereof.
Background
Animal farming and food processing industries are constantly facing concerns about microbial contamination, and the transmission of food-borne pathogens can cause the development of various food-borne diseases and serious food safety problems. The use of antibiotics or antimicrobial preservatives for foods is one of the key control methods to improve food safety. In recent years, with the increasing development of various side effects and antibiotic resistance problems of conventional synthetic antibacterial agents, there has been a great interest in natural compounds having antibacterial activity in the food and cultivation industries.
Natural bacteriostats can be classified into animal-derived, plant-derived and microorganism-derived bacteriostats according to their sources. For example, plant essential oils are considered to be an important source of natural antimicrobial compounds, and a variety of natural antimicrobial agents have been identified from natural essential oils, including thymol, menthol, and the like. The use of these compounds in the food industry has also been widely accepted, and they have been FDA classified as GRAS (generally recognized as safe). In addition, fatty acid is used as a natural antibacterial component, and is increasingly concerned by the food and animal breeding industries due to the characteristics of safety and effectiveness.
However, there are still some limitations to the use of these plant-derived natural antibacterial agents. Since these compounds are generally low in water solubility, it is necessary to dissolve them in a less polar organic solvent or disperse them in emulsion droplets or in a delivery system for biopolymer particles, thereby achieving an improvement in water solubility. But low loading levels may result in limited use of these materials as the primary antimicrobial in various products.
Disclosure of Invention
The invention aims to provide a natural bacteriostatic eutectic solvent-based nanoemulsion, a preparation method and application thereof, and the bacteriostatic nanoemulsion preparation has good stability and bacteriostatic performance.
In order to achieve the above purpose, the invention provides a nanoemulsion based on a natural bacteriostat eutectic solvent, which is characterized by comprising the following components in percentage by volume: 10-20% of hydrophobic eutectic solvent, 77-85% of water and 3-5% of emulsifying agent; wherein the hydrophobic eutectic solvent comprises: the molar ratio is 1: 2-3 of two kinds of C 8 ～C 12 Linear fatty acids, and the chain length of the linear fatty acids with large molar quantity is shorter than that of the linear fatty acids with small molar quantity; or, the molar ratio is 1:1 to 3C 8 ～C 12 Linear fatty acids and terpenoids, or in a molar ratio of 1: 1-3 of two terpenoids; wherein the terpenoid is selected from thymol, menthol or carvacrol; the emulsifier is selected from Tween 80, theasaponin or whey protein.
Preferably, the particle size of the nanoemulsion is 70-210 nm.
Preferably, the linear fatty acid is selected from capric acid, lauric acid or caprylic acid.
Preferably, the hydrophobic eutectic solvent comprises: the molar ratio is 1: 2-3 lauric acid and C 8 ～C 10 A linear fatty acid; or a molar ratio of 1:1 to 3C 8 ～C 10 Linear fatty acids and terpenoids.
Preferably, the hydrophobic eutectic solvent is decanoic acid and menthol in a molar ratio of 1:1, octanoic acid and menthol in a molar ratio of 1:1, decanoic acid and thymol in a molar ratio of 1:3, lauric acid and decanoic acid in a molar ratio of 1:2, lauric acid and octanoic acid in a molar ratio of 1:3, or thymol and menthol in a molar ratio of 1:1.
Preferably, the hydrophobic eutectic solvent is obtained by directly mixing the two components and forming a uniform liquid after stirring at 60 ℃.
Another object of the present invention is to provide a method for preparing the natural bacteriostat eutectic solvent-based nanoemulsion, which comprises the following steps: mixing a hydrophobic eutectic solvent, distilled water and an emulsifier, and shearing to prepare coarse emulsion; and carrying out intermittent ultrasonic or high-pressure homogenization or micro-jet homogenization treatment on the crude emulsion to obtain the stable nano emulsion.
Preferably, the shear rate is 13600prm.
Preferably, the intermittent ultrasonic wave has the power of 300W, the ultrasonic wave is started for 3s, the ultrasonic wave is stopped for 5s, and the total time is 5min; the pressure of the high-pressure homogenization is 100MPa; the pressure of the micro-jet homogenization is 50 MPa and 100MPa.
The invention also aims to provide an application of the nanoemulsion based on the natural bacteriostatic agent eutectic solvent in bacteriostasis in the food industry.
The nanoemulsion based on the natural bacteriostat eutectic solvent, and the preparation and the application thereof have the following advantages:
the nanoemulsion based on the natural bacteriostat eutectic solvent has good thermal stability and storage stability. In the aspect of thermal stability, the particle size of the emulsion is still kept around 200nm even the temperature is up to 90 ℃; for the storage stability of the emulsion, the change is still not obvious when the fifteenth day is reached, the particle size is not greatly increased, and the layering phenomenon is not generated. The terpene substances from the plant essential oil and the fatty acid from the plant oil are adopted as components, the eutectic synergistic bacteriostatic agent-hydrophobic eutectic solvent is prepared under the mixing of a certain proportion, and the eutectic synergistic bacteriostatic agent-hydrophobic eutectic solvent is subjected to ultrasonic emulsification to form a nano emulsion preparation, so that the problem of bacteriostatic concentration of the natural bacteriostatic agent when the natural bacteriostatic agent is singly implemented can be effectively solved, and a better bacteriostatic effect can be achieved through synergistic bacteriostasis. The nano emulsion provided by the invention has natural substances with a certain antibacterial effect, is environment-friendly, is oil-in-water type, can be conveyed in a water phase, exerts the antibacterial effect of fatty acid or terpenes, widens the application range of the nano emulsion, and can exert the antibacterial effect to be applied to the food industry.
Drawings
FIG. 1 is a flow chart of the preparation of the stable fatty acid-based nanoemulsion of the present invention.
FIG. 2 is a graph showing the effect of T80 concentration on the average particle size of nanoemulsions of examples 1-25 of the present invention.
FIG. 3 shows the average particle size results of the emulsions of the nanoemulsions of examples 1-5 of the present invention (T80: 5%) at various standing times.
FIG. 4 shows the average particle size results of the emulsions of the nanoemulsions of examples 1-5 of the present invention (T80: 5%) at different temperatures.
FIG. 5 is a graph showing E.coli growth under the effect of the nanoemulsion of the present invention and the control group.
Detailed Description
The following description of the technical solutions in the embodiments of the present invention will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
A nanoemulsion based on a natural bacteriostat eutectic solvent, the nanoemulsion comprising: a hydrophobic eutectic solvent, distilled water and an emulsifier tween 80 (T80), wherein the volume fraction of the hydrophobic eutectic solvent is 10%, the volume fraction of tween 80 is 5%, the volume fraction of distilled water is 85%, the hydrophobic eutectic solvent comprises: decanoic acid (Cap) and menthol (Men) in a molar ratio of 1:1.
The preparation method of the nanoemulsion based on the natural bacteriostat eutectic solvent is shown in fig. 1, and specifically comprises the following steps:
the corresponding mass is calculated according to the mole ratio, then the decanoic acid and the menthol are directly mixed, and the uniform liquid is formed after stirring for 20min at 60 ℃, so as to prepare the hydrophobic eutectic solvent which is in a liquid state at room temperature;
mixing hydrophobic eutectic solvent and distilled water, adding emulsifier Tween 80, and shearing at 13600prm for 2min to obtain coarse emulsion;
the crude emulsion is treated by intermittent ultrasonic (power 300W, ultrasonic on for 3s, ultrasonic off for 5s, total time 5 minutes) (ultrasonic twice under the same condition) to obtain stable nano emulsion.
Example 2
A nanoemulsion based on a natural bacteriostat eutectic solvent, substantially identical to that of example 1, except that: the hydrophobic eutectic solvent comprises: lauric acid (Lau) and capric acid (Cap) in a molar ratio of 1:2.
Example 3
A nanoemulsion based on a natural bacteriostat eutectic solvent, substantially identical to that of example 1, except that: the hydrophobic eutectic solvent comprises: octanoic acid (Cay) and menthol (Men) in a 1:1 molar ratio.
Example 4
A nanoemulsion based on a natural bacteriostat eutectic solvent, substantially identical to that of example 1, except that: the hydrophobic eutectic solvent comprises: lauric acid (Lau) and caprylic acid (Cay) in a molar ratio of 1:3.
Example 5
A nanoemulsion based on a natural bacteriostat eutectic solvent, substantially identical to that of example 1, except that: the hydrophobic eutectic solvent comprises: decanoic acid (Cap) and thymol (Thy) in a molar ratio of 2:1.
Examples 6 to 9
A nanoemulsion based on a natural bacteriostat eutectic solvent, substantially identical to that of example 1, except that: the volume fractions of tween 80 for examples 6-9 were 1%, 3%, 7% and 9%, respectively.
Examples 10 to 13
A nanoemulsion based on a natural bacteriostat eutectic solvent, substantially identical to example 2, except that: the volume fractions of tween 80 for examples 10-13 were 1%, 3%, 7% and 9%, respectively.
Examples 14 to 17
A nanoemulsion based on a natural bacteriostat eutectic solvent, substantially identical to example 3, except that: the volume fractions of tween 80 for examples 14-17 were 1%, 3%, 7% and 9%, respectively.
Examples 18 to 21
A nanoemulsion based on a natural bacteriostat eutectic solvent, substantially identical to example 4, except that: the volume fractions of tween 80 for examples 18-21 were 1%, 3%, 7% and 9%, respectively.
Examples 22 to 25
A nanoemulsion based on a natural bacteriostat eutectic solvent, substantially identical to example 5, except that: the volume fractions of tween 80 for examples 22-25 were 1%, 3%, 7% and 9%, respectively.
Example 26
A nanoemulsion based on a natural bacteriostat eutectic solvent, substantially identical to that of example 1, except that: the nanoemulsion comprises: a hydrophobic eutectic solvent, deionized water and an emulsifier theasaponin, wherein the volume fraction of the hydrophobic eutectic solvent is 20%, the volume fraction of the theasaponin is 3%, the volume fraction of deionized water is 77%, and the hydrophobic eutectic solvent comprises: lauric acid (Lau) and capric acid (Cap) in a molar ratio of 1:2.
The preparation method of the nano emulsion comprises the following steps:
mixing the components, and shearing at a high speed for 2min at 13600prm to prepare coarse emulsion;
and (3) carrying out high-pressure homogenization (100 MPa,5 times) on the crude emulsion to obtain the stable nano emulsion.
Example 27
A nanoemulsion based on a natural bacteriostat eutectic solvent, substantially identical to that of example 1, except that: the nanoemulsion comprises: a hydrophobic eutectic solvent, deionized water and an emulsifier tea saponin, wherein the volume fraction of the hydrophobic eutectic solvent is 10%, the volume fraction of the tea saponin is 5%, the volume fraction of the water is 85%, and the hydrophobic eutectic solvent comprises: thymol (Thy) and menthol (Men) in a molar ratio of 1:1.
The preparation method of the nano emulsion comprises the following steps:
mixing hydrophobic eutectic solvent and distilled water, adding theasaponin, and shearing at 13600prm for 2min to obtain coarse emulsion;
and carrying out micro-jet homogenization (50, 100MPa, 2-5 times) on the crude emulsion to obtain the stable nano emulsion.
Experimental example 1 particle size detection
As shown in FIG. 2, it is apparent from FIG. 2 that the average particle diameter of the nanoemulsion of examples 1-25 of the present invention is reduced as the concentration of T80 increases, the particle diameter is maximum at 1% concentration, the particle diameter is 200-350 nm, the particle diameter is stabilized at 7-9% concentration, the particle diameter is 50-150 nm, and the particle diameters of examples 5 and 22-25 are larger than those of other examples at the same concentration.
As shown in FIG. 3, the average particle size of the emulsions of examples 1-5 (T80: 5%) according to the present invention was obtained at different standing times, and it can be seen from FIG. 3 that the particle sizes of examples 1-5 were all substantially stable at 15 days of standing, and the particle sizes of the emulsions of examples 1-2 and 4-5 were substantially stable at 30 days of standing, and the particle size of example 3 was increased.
As shown in FIG. 4, the average particle size of the emulsions at various temperatures for the nanoemulsions of examples 1-5 of the present invention (T80: 5%) was found to be substantially constant from 30℃to 90℃as seen in FIG. 4.
From the above results, it can be seen that the nanoemulsion of the present invention has good stability.
Experimental example 2 antibacterial Properties
The Minimum Inhibitory Concentration (MIC) of the emulsion is found out by a double tube dilution method, then the escherichia coli is treated by the emulsion concentration with the size of 2MIC, a corresponding growth curve is made, and the OD value of the escherichia coli at 600nm is measured, so that the growth condition of a bacterial colony is reflected. Wherein, the blank control is distilled water treatment under the same amount. As shown in FIG. 5, the graph of the growth of the escherichia coli under the action of the nanoemulsion and the control group shows that the capric acid-menthol/capric acid-thymol nanoemulsion has remarkable antibacterial effect on the escherichia coli when the two groups of emulsions are compared with the control result.
The bacteriostasis performance results of nanoemulsions of examples 1 and 5 of the present invention are as follows:
while the present invention has been described in detail through the foregoing description of the preferred embodiment, it should be understood that the foregoing description is not to be considered as limiting the invention. Many modifications and substitutions of the present invention will become apparent to those of ordinary skill in the art upon reading the foregoing. Accordingly, the scope of the invention should be limited only by the attached claims.
Claims (10)
1. A nanoemulsion based on a natural bacteriostat eutectic solvent, which is characterized by comprising the following components in percentage by volume: 10-20% of a hydrophobic eutectic solvent, 77-85% of water and 5% of an emulsifier; wherein the hydrophobic eutectic solvent is: the molar ratio is 1: 2-3 two kinds of C 8 ~C 12 Linear fatty acids, and the chain length of the linear fatty acids with large molar quantity is shorter than that of the linear fatty acids with small molar quantity; or, the molar ratio is 1: c of 1 to 3 8 ~C 12 Linear fatty acids and terpenoids; wherein the terpenoid is selected from menthol or carvacrol;the emulsifier is selected from Tween 80 and theasaponin.
2. The natural bacteriostat eutectic solvent-based nanoemulsion of claim 1, wherein the particle size of the nanoemulsion is 70-210 nm.
3. Nanoemulsion based on natural bacteriostat eutectic solvents according to claim 1, characterized in that the linear fatty acids are chosen from capric acid, lauric acid or caprylic acid.
4. The natural bacteriostat eutectic solvent based nanoemulsion according to claim 1, wherein the hydrophobic eutectic solvent is: the molar ratio is 1: 2-3 lauric acid and C 8 ~C 10 A linear fatty acid; or a molar ratio of 1: c of 1 to 3 8 ~C 10 Linear fatty acids and terpenoids.
5. The natural bacteriostat eutectic solvent based nanoemulsion according to claim 1, wherein the hydrophobic eutectic solvent is decanoic acid and menthol in a molar ratio of 1:1, or octanoic acid and menthol in a molar ratio of 1:1, or lauric acid and decanoic acid in a molar ratio of 1:2, or lauric acid and octanoic acid in a molar ratio of 1:3.
6. The natural bacteriostat eutectic solvent based nanoemulsion according to claim 1, wherein the hydrophobic eutectic solvent is obtained by directly mixing two components and forming a uniform liquid after stirring at 60 ℃.
7. A method for preparing a natural bacteriostat eutectic solvent based nanoemulsion according to any one of claims 1-6, wherein the method comprises: mixing a hydrophobic eutectic solvent, distilled water and an emulsifier, and shearing to prepare coarse emulsion; and carrying out intermittent ultrasonic or high-pressure homogenization or micro-jet homogenization treatment on the crude emulsion to obtain the stable nano emulsion.
8. The method of claim 7, wherein the shear is at a speed of 13600 rpm.
9. The method of claim 7, wherein the intermittent ultrasound is at 300W power, ultrasound on 3s, off 5s, total time 5min; the pressure of the high-pressure homogenization is 100MPa; the pressure of the micro-jet homogenization is 50 MPa and 100MPa.
10. Use of a nanoemulsion based on natural bacteriostat eutectic solvents according to any one of claims 1-6 for bacteriostasis in the food industry.
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