CN112592892A - Culture medium and culture method for inducing secretion of umbilical cord mesenchymal stem cell factors - Google Patents
Culture medium and culture method for inducing secretion of umbilical cord mesenchymal stem cell factors Download PDFInfo
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
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- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/485—Epidermal growth factor [EGF] (urogastrone)
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/495—Transforming growth factor [TGF]
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/50—Fibroblast growth factors [FGF]
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- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/999—Small molecules not provided for elsewhere
Abstract
The invention discloses a culture medium for inducing the secretion of umbilical cord mesenchymal stem cell factors, which comprises a basic culture medium and gossypol acetate, meglumine, dextran and dipotassium glycyrrhizinate which are added into the basic culture medium. According to the culture medium for inducing the secretion of the umbilical cord mesenchymal stem cell factor, gossypol acetate, meglumine, dextran and dipotassium glycyrrhizinate are added into a basic culture medium, and the components have synergistic effects, so that the secretion of the cell factor can be effectively promoted in the induction culture process of the umbilical cord mesenchymal stem cell, the content of the cell factor in a cell culture solution is improved, and a guarantee is provided for the application of an umbilical cord mesenchymal stem cell factor product in the field of beauty and skin care. The invention also provides a culture method for inducing the secretion of the umbilical cord mesenchymal stem cell factor, the induction culture medium is adopted, the umbilical cord mesenchymal stem cells subjected to subculture are taken as an induction object, the whole process is easy to operate, the cost is saved, and the method is convenient for commercial production of various cell factor products.
Description
Technical Field
The invention relates to the field of stem cells, in particular to a culture medium and a culture method for inducing the secretion of umbilical cord mesenchymal stem cell factors.
Background
Mesenchymal stem cells are important members of the stem cell family, derived from the mesoderm and ectoderm in early developmental stages. Mesenchymal stem cells were first found in bone marrow and are increasingly receiving attention due to their characteristics such as multiple differentiation potential, hematopoietic support, promotion of stem cell engraftment, immunoregulation and self-replication. The bone marrow mesenchymal stem cells are widely applied clinically, and the current research shows that the umbilical cord-derived mesenchymal stem cells also have greater application potential.
The human umbilical cord mesenchymal stem cells express the unique marks of various stem cells, and have the characteristics of large differentiation potential, strong proliferation capacity, low immunogenicity, convenient material taking, no ethical problem limitation, easy industrial preparation and the like. The culture supernatant of the human umbilical cord mesenchymal stem cells contains a large amount of cell growth factors, such as Epidermal Growth Factor (EGF), Fibroblast Growth Factor (FGF), Keratinocyte Growth Factor (KGF), transforming growth factor beta (TGF-beta), Vascular Endothelial Growth Factor (VEGF) and the like, and the cell factors play an important role in promoting the metabolism of fibroblasts and the formation of collagen, can effectively promote the metabolism of skin cells and promote the growth, differentiation and repair of the skin cells.
The method for obtaining the cell factor is that the umbilical cord mesenchymal stem cells are directly used in umbilical cord mesenchymal stem cell culture solution after being cultured, so that the loss of effective components in the repeated operation process can be reduced, but the in-vitro amplification of the umbilical cord mesenchymal stem cells is carried out in a culture medium supplemented with fetal calf serum or supplemented with human autologous serum or basically similar serum. Bovine serum, human serum or other animal serums may contain blood-transmitted pathogens, the bovine serum can also stimulate the generation of an anti-foreign biomass protein antibody, the heterotypic biomass protein can stimulate the immune response of a receptor patient, the potential safety hazard of a cytokine product is increased, and because the content of the cytokine in the culture solution is lower, in order to realize corresponding effects, a large amount of cell culture is often required, the process is time-consuming and labor-consuming, therefore, the culture medium is necessary to be improved, so as to induce umbilical cord mesenchymal stem cells to secrete more cytokines, the production cost is reduced, and the safety of the cytokine product is improved.
Disclosure of Invention
In order to overcome the defects of the prior art, one of the purposes of the invention is to provide a culture medium for inducing the secretion of umbilical cord mesenchymal stem cell factors, effectively promote the umbilical cord mesenchymal stem cells to secrete the cell factors, and improve the content of the cell factors in a cell culture solution.
The invention also aims to provide a culture method for inducing the secretion of the umbilical cord mesenchymal stem cell factor.
One of the purposes of the invention is realized by adopting the following technical scheme:
a culture medium for inducing the secretion of umbilical cord mesenchymal stem cell factor includes basic culture medium and gossypol acetate, meglumine, dextran and dipotassium glycyrrhizinate added in the basic culture medium.
Further, the dosage of each component is as follows according to the final concentration of the culture medium: 45.3-50.5 mug/mL of gossypol acetate, 78.5-90.0ng/mL of meglumine, 23.8-28.2 mug/mL of dextran and 1-5mg/mL of dipotassium glycyrrhizinate.
Further, the dosage of each component is as follows according to the final concentration of the culture medium: 47.5 mu g/mL of gossypol acetate, 84.5ng/mL of meglumine, 25.8 mu g/mL of dextran and 3mg/mL of dipotassium glycyrrhizinate.
Further, the basic medium is DMEM/F12 medium.
Further, the volume ratio of DMEM to F12 in the basal medium is 1: 1.
The second purpose of the invention is realized by adopting the following technical scheme:
a culture method for inducing the secretion of umbilical cord mesenchymal stem cell factors comprises the following steps: and inoculating the umbilical cord mesenchymal stem cells subjected to subculture into the culture medium for induction culture.
Further, the seeding density of the umbilical cord mesenchymal stem cells in the culture medium is 1-2 x 105one/mL.
Further, the induction culture process was carried out at 37 ℃ with 5% CO2Under the conditions of (1).
Further, the umbilical cord mesenchymal stem cells are cells subcultured to P3-P5 passages.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention provides a culture medium for inducing the secretion of umbilical cord mesenchymal stem cell factors, wherein gossypol acetate, meglumine, dextran and dipotassium glycyrrhizinate are added into a basic culture medium, and the components have synergistic effect, so that the secretion of the cell factors can be effectively promoted in the induction culture process of umbilical cord mesenchymal stem cells, the content of the cell factors in a cell culture solution is improved, and a guarantee is provided for the application of umbilical cord mesenchymal stem cell factor products in the field of beauty and skin care.
2. The invention also provides a culture method for inducing the secretion of the umbilical cord mesenchymal stem cell factor, the induction culture medium is adopted, the umbilical cord mesenchymal stem cells subjected to subculture are taken as an induction object, the whole process is easy to operate, the cost is saved, and the method is convenient for commercial production of various cell factor products.
Detailed Description
The present invention is further described below with reference to specific embodiments, and it should be noted that, without conflict, any combination between the embodiments or technical features described below may form a new embodiment.
The umbilical cord mesenchymal stem cells used for induction culture in examples 1 to 3 were prepared by the following method:
(1) collecting umbilical cord tissues of healthy human fetuses delivered in term, cutting into small sections of 1-2cm in a super clean bench, and fully cleaning with normal saline;
(2) cutting off small segments of umbilical cord tissue, removing vein vessels, and only retaining white connective tissue Wharton's jelly between amnion and blood vessel;
(3) shearing the Wharton's jelly to about 1-2mm3The tissue blocks with the same volume size are washed 3 times with physiological saline and inoculated on a tissue block with an area of 55mm210mL of lonza 12-725F medium was added to the sterile petri dish, and the mixture was left at 37 ℃ with 5% CO2Culturing;
(4) and (3) performing half-amount liquid change the next day after the cells are attached to the wall, discarding the cells which are not attached to the wall, performing liquid change once every 3 days, removing tissue blocks after the cell fusion degree reaches 80%, digesting with 0.25% pancreatin, and performing subculture on the digested cells as P0 generation cells according to the proportion of 1: 3.
Example 1
A culture medium for inducing the secretion of umbilical cord mesenchymal stem cell factors comprises a basic culture medium DMEM/F12 (the volume ratio of DMEM to F12 is 1:1), gossypol acetate, meglumine, dextran and dipotassium glycyrrhizinate which are added into the basic culture medium, and the dosage of each component is as follows according to the final concentration of the culture medium: 47.5 mu g/mL of gossypol acetate, 84.5ng/mL of meglumine, 25.8 mu g/mL of dextran and 3mg/mL of dipotassium glycyrrhizinate.
A culture method for inducing the secretion of umbilical cord mesenchymal stem cell factors comprises the following steps: washing umbilical cord mesenchymal stem cells subcultured to P3 generation with PBS, inoculating the cells into the medium for inducing the secretion of umbilical cord mesenchymal stem cell factors in a T75 culture flask, wherein the inoculation density of the cells is 1.5 multiplied by 105one/mL, 5% CO at 37 ℃2The induction culture was carried out in the incubator of (1) for 3 days.
Example 2
A culture medium for inducing the secretion of umbilical cord mesenchymal stem cell factors comprises a basic culture medium DMEM/F12 (the volume ratio of DMEM to F12 is 1:1), gossypol acetate, meglumine, dextran and dipotassium glycyrrhizinate which are added into the basic culture medium, and the dosage of each component is as follows according to the final concentration of the culture medium: 45.3 mu g/mL of gossypol acetate, 78.5ng/mL of meglumine, 23.8 mu g/mL of dextran and 1mg/mL of dipotassium glycyrrhizinate.
A culture method for inducing the secretion of umbilical cord mesenchymal stem cell factors comprises the following steps: washing umbilical cord mesenchymal stem cells subcultured to P4 generation with PBS, inoculating the cells into the medium for inducing the secretion of umbilical cord mesenchymal stem cell factors in a T75 culture flask, wherein the inoculation density of the cells is 1.0 × 105one/mL, 5% CO at 37 ℃2The induction culture was carried out in the incubator of (1) for 3 days.
Example 3
A culture medium for inducing the secretion of umbilical cord mesenchymal stem cell factors comprises a basic culture medium DMEM/F12 (the volume ratio of DMEM to F12 is 1:1), gossypol acetate, meglumine, dextran and dipotassium glycyrrhizinate which are added into the basic culture medium, and the dosage of each component is as follows according to the final concentration of the culture medium: 50.5 mu g/mL of gossypol acetate, 90.0ng/mL of meglumine, 28.2 mu g/mL of dextran and 5mg/mL of dipotassium glycyrrhizinate.
A culture method for inducing the secretion of umbilical cord mesenchymal stem cell factors comprises the following steps: washing umbilical cord mesenchymal stem cells subcultured to P5 generation with PBS, inoculating the cells into the medium for inducing the secretion of umbilical cord mesenchymal stem cell factors in a T75 culture flask, wherein the inoculation density of the cells is 2 multiplied by 105one/mL, 5% CO at 37 ℃2The induction culture was carried out in the incubator of (1) for 3 days.
Comparative example 1
Comparative example 1 provides a medium for inducing secretion of umbilical cord mesenchymal stem cell factor, which is different from example 1 in that gossypol acetate is omitted, and the rest is the same as example 1.
Comparative example 2
Comparative example 2 provides a medium for inducing the secretion of umbilical cord mesenchymal stem cell factor, which is different from example 1 in that meglumine is omitted, and the others are the same as example 1.
Comparative example 3
Comparative example 3 provides a medium for inducing secretion of umbilical cord mesenchymal stem cell factor, which is different from example 1 in that dextran is omitted, and the other steps are the same as example 1.
Comparative example 4
Comparative example 4 provides a medium for inducing secretion of umbilical cord mesenchymal stem cell factor, which is different from example 1 in that dipotassium glycyrrhizinate is omitted, and the rest is the same as example 1.
Comparative example 5
Comparative example 5 provides a medium for inducing secretion of umbilical cord mesenchymal stem cell factor, which is different from example 1 in that gossypol acetate, meglumine, dextran, dipotassium glycyrrhizinate are omitted, and the others are the same as example 1.
The culture fluids of examples 1 to 3 and comparative examples 1 to 5 were centrifuged at 1000rpm for 5min, the supernatant was collected after centrifugation, and then dialyzed through a 50KD ultrafiltration membrane, and the dialysate was further passed through a 100D ultrafiltration membrane, and the retentate was collected, and among which Fibroblast Growth Factor (FGF), Epidermal Growth Factor (EGF), Vascular Endothelial Growth Factor (VEGF), Keratinocyte Growth Factor (KGF), and transforming growth factor beta (TGF-. beta.) were measured using an ELISA kit, and the results are shown in Table 1.
TABLE 1
| Sample (I) | FGF(ng/mL) | EGF(ng/mL) | VEGF(ng/mL) | KGF(ng/mL) | TGF-β(ng/mL) |
| Example 1 | 131.32 | 79.32 | 68.33 | 83.25 | 75.83 |
| Example 2 | 120.49 | 62.15 | 59.72 | 72.56 | 63.15 |
| Example 3 | 124.36 | 66.32 | 63.95 | 78.35 | 71.44 |
| Comparative example 1 | 62.57 | 37.24 | 35.18 | 41.23 | 32.24 |
| Comparative example 2 | 71.24 | 45.79 | 42.02 | 48.61 | 40.51 |
| Comparative example 3 | 65.31 | 41.22 | 38.79 | 44.30 | 36.49 |
| Comparative example 4 | 74.96 | 48.17 | 45.99 | 51.23 | 43.55 |
| Comparative example 5 | 38.62 | 21.03 | 19.83 | 28.11 | 26.78 |
As is clear from Table 1, the cell culture liquids obtained in examples 1 to 3 had higher cytokine contents than those obtained in comparative examples 1 to 5. The composition of the induction culture medium is adjusted in comparative examples 1 to 5, one or more of gossypol acetate, meglumine, dextran and dipotassium glycyrrhizinate are respectively omitted, and the content of the cell factors in the cell culture solution finally obtained through induction culture is reduced to different degrees.
The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.
Claims (9)
1. A culture medium for inducing the secretion of umbilical cord mesenchymal stem cell factors is characterized by comprising a basic culture medium and gossypol acetate, meglumine, dextran and dipotassium glycyrrhizinate which are added into the basic culture medium.
2. The medium for inducing the secretion of the umbilical cord mesenchymal stem cell factor according to claim 1, wherein the medium comprises the following components in terms of final concentration of the medium: 45.3-50.5 mug/mL of gossypol acetate, 78.5-90.0ng/mL of meglumine, 23.8-28.2 mug/mL of dextran and 1-5mg/mL of dipotassium glycyrrhizinate.
3. The medium for inducing the secretion of the umbilical cord mesenchymal stem cell factor according to claim 1, wherein the medium comprises the following components in terms of final concentration of the medium: 47.5 mu g/mL of gossypol acetate, 84.5ng/mL of meglumine, 25.8 mu g/mL of dextran and 3mg/mL of dipotassium glycyrrhizinate.
4. The medium for inducing secretion of umbilical cord mesenchymal stem cell factor according to claim 1, wherein the basic medium is DMEM/F12 medium.
5. The culture medium for inducing secretion of umbilical cord mesenchymal stem cell factor according to claim 4, wherein the volume ratio of DMEM and F12 in the basic culture medium is 1: 1.
6. A culture method for inducing the secretion of umbilical cord mesenchymal stem cell factors is characterized by comprising the following steps: inoculating the umbilical cord mesenchymal stem cells subjected to subculture into the culture medium of claims 1 to 5 for induction culture.
7. The culture method for inducing secretion of umbilical cord mesenchymal stem cell factors according to claim 6, wherein the seeding density of umbilical cord mesenchymal stem cells in the culture medium is 1-2 x 105one/mL.
8. The culture method for inducing secretion of umbilical cord mesenchymal stem cell factors according to claim 6, wherein the culture process is induced at 37 ℃ and 5% CO2Under the conditions of (1).
9. The culture method for inducing secretion of umbilical cord mesenchymal stem cell factors according to claim 6, wherein the umbilical cord mesenchymal stem cells are cells subcultured to P3-P5 generation.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113151165A (en) * | 2021-05-14 | 2021-07-23 | 河北驰熙科技发展有限公司 | Culture medium and culture method for human umbilical cord mesenchymal stem cell amplification |
| CN116410921A (en) * | 2023-02-09 | 2023-07-11 | 北京益华生物科技有限公司 | Human umbilical cord mesenchymal stem cell induction culture medium, induction method and application |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013165252A1 (en) * | 2012-05-03 | 2013-11-07 | Erasmus University Medical Center Rotterdam | Culturing of mesenchymal stem cells |
| CN106754639A (en) * | 2016-12-08 | 2017-05-31 | 北京银丰鼎诚生物工程技术有限公司 | A kind of mescenchymal stem cell factor large-scale producing method |
| CN107260570A (en) * | 2017-06-14 | 2017-10-20 | 南京仯素生物科技有限公司 | A kind of mescenchymal stem cell factor composition and its production and use |
| CN110448572A (en) * | 2019-08-04 | 2019-11-15 | 丰泽康生物医药（深圳）有限公司 | A kind of preparation method of umbilical cord mesenchymal stem cells active matter and the compound of cord blood stem cell active matter |
| CN110693804A (en) * | 2019-10-10 | 2020-01-17 | 湖南源品细胞生物科技有限公司 | Facial mask containing umbilical cord mesenchymal stem cell factor and preparation method thereof |
| WO2020204161A1 (en) * | 2019-04-04 | 2020-10-08 | 日産化学株式会社 | Composition for promoting secretion of extracellular vesicles |
-
2020
- 2020-12-25 CN CN202011564772.XA patent/CN112592892B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013165252A1 (en) * | 2012-05-03 | 2013-11-07 | Erasmus University Medical Center Rotterdam | Culturing of mesenchymal stem cells |
| CN106754639A (en) * | 2016-12-08 | 2017-05-31 | 北京银丰鼎诚生物工程技术有限公司 | A kind of mescenchymal stem cell factor large-scale producing method |
| CN107260570A (en) * | 2017-06-14 | 2017-10-20 | 南京仯素生物科技有限公司 | A kind of mescenchymal stem cell factor composition and its production and use |
| WO2020204161A1 (en) * | 2019-04-04 | 2020-10-08 | 日産化学株式会社 | Composition for promoting secretion of extracellular vesicles |
| CN110448572A (en) * | 2019-08-04 | 2019-11-15 | 丰泽康生物医药（深圳）有限公司 | A kind of preparation method of umbilical cord mesenchymal stem cells active matter and the compound of cord blood stem cell active matter |
| CN110693804A (en) * | 2019-10-10 | 2020-01-17 | 湖南源品细胞生物科技有限公司 | Facial mask containing umbilical cord mesenchymal stem cell factor and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 刘斌: "《细胞培养》", 31 January 2018, 世界图书出版公司 * |
| 周荣等: "环磷酸腺苷葡甲胺诱导骨髓间充质干细胞向心肌细胞分化的实验研究", 《中国病理生理杂志》 * |
| 许士凯: "《新编药物手册》", 31 August 2003, 上海科技教育出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113151165A (en) * | 2021-05-14 | 2021-07-23 | 河北驰熙科技发展有限公司 | Culture medium and culture method for human umbilical cord mesenchymal stem cell amplification |
| CN113151165B (en) * | 2021-05-14 | 2022-06-21 | 山西北科生物科技有限公司 | Culture medium and culture method for human umbilical cord mesenchymal stem cell amplification |
| CN116410921A (en) * | 2023-02-09 | 2023-07-11 | 北京益华生物科技有限公司 | Human umbilical cord mesenchymal stem cell induction culture medium, induction method and application |
| CN116410921B (en) * | 2023-02-09 | 2024-01-23 | 北京益华生物科技有限公司 | Human umbilical cord mesenchymal stem cell induction culture medium, induction method and application |
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