CN112481206A - Culture medium and culture method for inducing secretion of adipose-derived mesenchymal stem cell factor - Google Patents
Culture medium and culture method for inducing secretion of adipose-derived mesenchymal stem cell factor Download PDFInfo
- Publication number
- CN112481206A CN112481206A CN202011487212.9A CN202011487212A CN112481206A CN 112481206 A CN112481206 A CN 112481206A CN 202011487212 A CN202011487212 A CN 202011487212A CN 112481206 A CN112481206 A CN 112481206A
- Authority
- CN
- China
- Prior art keywords
- adipose
- mesenchymal stem
- culture medium
- derived mesenchymal
- stem cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/40—Nucleotides, nucleosides, bases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/70—Undefined extracts
- C12N2500/76—Undefined extracts from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/90—Polysaccharides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Rheumatology (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a culture medium for inducing adipose-derived mesenchymal stem cell factor secretion, which comprises a basic culture medium, and zeatin, transferrin, caffeine and sodium alginate which are added in the basic culture medium. The components have synergistic effect, and can effectively promote the secretion of cell factors in the induction culture process of the adipose mesenchymal stem cells, improve the content of the cell factors in the separated secretion, and further improve the content of effective components in the adipose mesenchymal stem cell factor product. The induction culture medium disclosed by the invention does not need low-oxygen environment stimulation in the induction process, is simple and convenient to operate, and provides guarantee for the application of adipose mesenchymal stem cell factor products in the fields of wound repair and the like. The invention also provides a culture method for inducing the secretion of the adipose-derived mesenchymal stem cell factor, the induction culture medium is adopted, the adipose-derived mesenchymal stem cells subjected to subculture are taken as an induction object, the whole process is easy to operate, the cost is saved, and the method is convenient for the commercial production of various cytokine products.
Description
Technical Field
The invention relates to the field of stem cells, in particular to a culture medium and a culture method for inducing the secretion of adipose-derived mesenchymal stem cell factors.
Background
Stem cells are a type of primitive and unspecified pluripotent cells with the ability to self-replicate. Under certain conditions, it can differentiate into a variety of functional cells. Adipose-derived mesenchymal stem cells are an important type of stem cells, and the adipose-derived mesenchymal stem cells exert effects on surrounding neighboring cells by secreting various cytokines. Adipose-derived stem cells can secrete various cytokines such as Fibroblast Growth Factor (FGF), Epidermal Growth Factor (EGF), transforming growth factor beta 1 (TGF-beta 1), platelet-derived growth factor (PDGF), Vascular Endothelial Growth Factor (VEGF), and the like.
The human adipose-derived mesenchymal stem cells are cultured, and the secreted bioactive factors are collected, extracted and concentrated to prepare the human adipose-derived mesenchymal stem cell extract product which is mainly applied to medicines with the functions of promoting wound healing and scar repair. Still other adipose-derived stem cell factor products are obtained by directly lysing stem cells, and have complex protein components, high concentrations of ineffective proteins, and metabolic stress on the human body.
Therefore, in order to ensure the safety of clinical application and increase the content of the cytokine in the product, the existing culture medium and culture process for performing cytokine induction culture on the adipose-derived mesenchymal stem cells need to be improved, which is helpful for developing adipose-derived mesenchymal stem cell complex factor products suitable for clinical application and large-scale industrial production.
Disclosure of Invention
In order to overcome the defects of the prior art, one of the purposes of the invention is to provide a culture medium for inducing the secretion of adipose-derived mesenchymal stem cell factors, which does not need the stimulation of a low-oxygen environment, effectively promotes the adipose-derived mesenchymal stem cells to secrete the cell factors, and improves the content of the cell factors in cell secretions.
The invention also aims to provide a culture method for inducing the secretion of the adipose-derived mesenchymal stem cell factor.
One of the purposes of the invention is realized by adopting the following technical scheme:
a culture medium for inducing the secretion of adipose-derived stem cell factor comprises a basic culture medium, and zeatin, transferrin, caffeine and sodium alginate added in the basic culture medium.
Further, the dosage of each component is as follows according to the final concentration of the culture medium: zeatin 18.4-24.7mg/mL, transferrin 9.5-13.0mg/mL, caffeine 40.5-47.2 μ g/mL, and sodium alginate 50.0-54.5 μ g/mL.
Further, the dosage of each component is as follows according to the final concentration of the culture medium: zeatin 22.5mg/mL, transferrin 11.8 mg/mL, caffeine 44.5 μ g/mL, and sodium alginate 52.7 μ g/mL.
Further, the basic medium is DMEM/F12 medium.
Further, the volume ratio of DMEM to F12 in the basal medium is 1: 1.
The second purpose of the invention is realized by adopting the following technical scheme:
a culture method for inducing the secretion of adipose-derived mesenchymal stem cell factors comprises the following steps: and inoculating the adipose-derived mesenchymal stem cells subjected to subculture into the culture medium for induction culture.
Further, the inoculation density of the adipose-derived mesenchymal stem cells in the induction medium is 1-2 x 105one/mL.
Further, the induction culture process was carried out at 37 ℃ with 5% CO2Under the conditions of (1).
Further, the adipose-derived mesenchymal stem cells are cells subcultured to P3-P5 generations.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention provides a culture medium for inducing adipose-derived mesenchymal stem cell factor secretion, which can effectively promote the secretion of cell factors in the induced culture process of adipose-derived mesenchymal stem cells by adding zeatin, transferrin, caffeine and sodium alginate into a basic culture medium and realizing the synergistic effect of all the components, thereby improving the content of the cell factors in the separated secretion and further improving the content of effective components in an adipose-derived mesenchymal stem cell factor product. By adopting the induction culture medium, the stimulation of a low-oxygen environment is not needed in the induction process, the operation is simple and convenient, and the application of the adipose-derived mesenchymal stem cell factor product in the fields of wound repair and the like is guaranteed.
2. The invention also provides a culture method for inducing the secretion of the adipose-derived mesenchymal stem cell factor, the induction culture medium is adopted, the adipose-derived mesenchymal stem cells subjected to subculture are taken as an induction object, the whole process is easy to operate, the cost is saved, and the method is convenient for the commercial production of various cytokine products.
Detailed Description
The present invention is further described below with reference to specific embodiments, and it should be noted that, without conflict, any combination between the embodiments or technical features described below may form a new embodiment.
The umbilical cord mesenchymal stem cells subcultured in the embodiments 1 to 3 of the present invention are prepared by the following method:
(1) taking adipose tissues of healthy donors, adding normal saline into the collected adipose tissues, cleaning the adipose tissues, standing for layering, sucking out lower-layer liquid, repeating the process, and cleaning for three times;
(2) cutting adipose tissue to about 1-2mm3The small pieces are uniformly spread on 100mm2The bottom of the culture dish was filled with 8mL serum-free LONZA fat medium per dish at 37 deg.C, 5% CO2Culturing in an incubator, wherein the culture medium is replaced every 3 days in the culture process;
(3) Observing cell growth with an inverted microscope, when cell confluence reaches 60%, sucking out supernatant and adipose tissue, and adding 0.25% pancreatin for digestion;
(4) adding 4mL of culture medium into the digested cells, transferring the cells into a centrifuge tube for centrifugation, discarding the supernatant, adding 8mL of culture medium for resuspension, and subculturing the cells as P0 generation cells, wherein the concentration of the subcultured cells is 2X 104one/mL in a ratio of 1: 4.
Example 1
A culture medium for inducing the secretion of adipose-derived mesenchymal stem cell factors comprises a basic culture medium DMEM/F12 (the volume ratio of DMEM to F12 is 1: 1), zeatin, transferrin, caffeine and sodium alginate which are added into the basic culture medium, and the dosage of each component is as follows according to the final concentration of the culture medium: zeatin 22.5mg/mL, transferrin 11.8 mg/mL, caffeine 44.5 μ g/mL, and sodium alginate 52.7 μ g/mL.
A culture method for inducing the secretion of adipose-derived mesenchymal stem cell factors comprises the following steps: culturing adipose-derived mesenchymal stem cells subcultured to P3 generation to cell growth density of 40%, discarding the original culture medium, washing with PBS, inoculating the cells into the above culture medium for inducing secretion of adipose-derived mesenchymal stem cell factor in T75 culture flask, wherein the inoculation density of the cells is 1.5 × 10 5one/mL, 5% CO at 37 ℃2The induction culture was carried out in the incubator of (1) for 3 days.
Example 2
A culture medium for inducing the secretion of adipose-derived mesenchymal stem cell factors comprises a basic culture medium DMEM/F12 (the volume ratio of DMEM to F12 is 1: 1), zeatin, transferrin, caffeine and sodium alginate which are added into the basic culture medium, and the dosage of each component is as follows according to the final concentration of the culture medium: zeatin 18.4mg/mL, transferrin 9.5 mg/mL, caffeine 40.5 μ g/mL, and sodium alginate 50.0 μ g/mL.
A culture method for inducing the secretion of adipose-derived mesenchymal stem cell factors comprises the following steps: culturing adipose-derived mesenchymal stem cells subcultured to P4 generation to cell growth density of 45%, discarding the original culture medium, washing with PBS, inoculating the cells into the above culture medium for inducing secretion of adipose-derived mesenchymal stem cell factor in T75 culture flask, wherein the inoculation density of the cells is 1 × 105one/mL, 5% CO at 37 ℃2The induction culture was carried out in the incubator of (2) for 2 days.
Example 3
A culture medium for inducing the secretion of adipose-derived mesenchymal stem cell factors comprises a basic culture medium DMEM/F12 (the volume ratio of DMEM to F12 is 1: 1), zeatin, transferrin, caffeine and sodium alginate which are added into the basic culture medium, and the dosage of each component is as follows according to the final concentration of the culture medium: 24.7mg/mL of zeatin, 13.0 mg/mL of transferrin, 47.2 mu g/mL of caffeine and 54.5 mu g/mL of sodium alginate.
A culture method for inducing the secretion of adipose-derived mesenchymal stem cell factors comprises the following steps: culturing adipose-derived mesenchymal stem cells subcultured to P5 generation until cell growth density reaches 50%, discarding original culture medium, washing with PBS, inoculating the cells into the above culture medium for inducing secretion of adipose-derived mesenchymal stem cell factor in T75 culture flask, wherein the inoculation density of the cells is 2 × 105one/mL, 5% CO at 37 ℃2The induction culture was carried out in the incubator of (1) for 3 days.
Comparative example 1
Comparative example 1 provides a medium for inducing secretion of adipose-derived mesenchymal stem cell factor, which is different from example 1 in that: zeatin was omitted and the procedure was as in example 1.
Comparative example 2
Comparative example 2 provides a medium for inducing secretion of adipose-derived mesenchymal stem cell factor, which is different from example 1 in that: transferrin is omitted and the rest is the same as in example 1.
Comparative example 3
Comparative example 3 provides a medium for inducing secretion of adipose-derived mesenchymal stem cell factor, which is different from example 1 in that: caffeine was omitted and the same as in example 1.
Comparative example 4
Comparative example 4 provides a medium for inducing secretion of adipose-derived mesenchymal stem cell factor, which is different from example 1 in that: the sodium alginate was omitted and the procedure was as in example 1.
Comparative example 5
Comparative example 5 provides a medium for inducing secretion of adipose-derived mesenchymal stem cell factor, which is different from example 1 in that: zeatin, transferrin, caffeine and sodium alginate are omitted, and the rest is the same as in example 1.
And (3) centrifuging the supernatant obtained after the induction culture of the cells in the examples 1 to 3 and the comparative examples 1 to 5 at 1000rpm for 5min, collecting the supernatant after centrifugation, dialyzing through an ultrafiltration membrane with the density of 30KD, collecting the dialysate through an ultrafiltration membrane with the density of 90D, collecting the trapped fluid, filtering the trapped fluid through a filter membrane with the size of 0.22 mu m, sterilizing and freeze-drying to obtain the adipose-derived mesenchymal stem cell factor product. The results of detection of Fibroblast Growth Factor (FGF), Epidermal Growth Factor (EGF), transforming growth factor beta 1 (TGF-beta 1), platelet-derived growth factor (PDGF) and Vascular Endothelial Growth Factor (VEGF) in adipose-derived stem cell factor products by ELISA kit are shown in table 1.
TABLE 1
| Sample (I) | FGF（ng/mL） | EGF（ng/mL） | TGF-β1（ng/mL） | PDGF（ng/mL） | VEGF（ng/mL） |
| Example 1 | 172.25 | 67.32 | 45.25 | 51.49 | 81.79 |
| Example 2 | 165.74 | 63.19 | 40.94 | 45.26 | 77.32 |
| Example 3 | 169.17 | 65.64 | 43.86 | 48.54 | 79.09 |
| Comparative example 1 | 72.34 | 35.46 | 23.16 | 28.49 | 57.96 |
| Comparative example 2 | 85.79 | 40.75 | 30.05 | 33.72 | 61.57 |
| Comparative example 3 | 81.18 | 38.12 | 27.67 | 30.04 | 60.01 |
| Comparative example 4 | 97.87 | 45.74 | 33.17 | 38.19 | 65.35 |
| Comparative example 5 | 52.17 | 19.21 | 12.84 | 20.31 | 35.42 |
As can be seen from table 1, after the main cytokines obtained from each group were measured, the contents of various adipose-derived mesenchymal stem cells finally obtained in examples 1 to 3 were higher than those of comparative examples 1 to 5. In comparative examples 1 to 5, one or more of zeatin, transferrin, caffeine and sodium alginate are respectively omitted, and the prepared induction culture medium is used for induction culture of adipose mesenchymal stem cells, so that the content of cytokines is reduced to different degrees.
The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.
Claims (9)
1. A culture medium for inducing the secretion of adipose-derived mesenchymal stem cell factors is characterized by comprising a basic culture medium, and zeatin, transferrin, caffeine and sodium alginate which are added into the basic culture medium.
2. The culture medium for inducing the secretion of the adipose-derived mesenchymal stem cell factor according to claim 1, wherein the final concentration of the culture medium comprises the following components: zeatin 18.4-24.7mg/mL, transferrin 9.5-13.0mg/mL, caffeine 40.5-47.2 μ g/mL, and sodium alginate 50.0-54.5 μ g/mL.
3. The culture medium for inducing the secretion of the adipose-derived mesenchymal stem cell factor according to claim 1, wherein the final concentration of the culture medium comprises the following components: zeatin 22.5mg/mL, transferrin 11.8 mg/mL, caffeine 44.5 μ g/mL, and sodium alginate 52.7 μ g/mL.
4. The medium for inducing secretion of adipose-derived mesenchymal stem cell factors according to claim 1, wherein the basic medium is DMEM/F12 medium.
5. The culture medium for inducing secretion of adipose-derived mesenchymal stem cell factors according to claim 1, wherein the volume ratio of DMEM to F12 in the basic culture medium is 1: 1.
6. A culture method for inducing the secretion of adipose-derived mesenchymal stem cell factors is characterized by comprising the following steps: inoculating the subcultured adipose-derived mesenchymal stem cells into the culture medium according to any one of claims 1 to 5 for induction culture.
7. The culture method for inducing secretion of adipose-derived mesenchymal stem cell factors according to claim 6, wherein the seeding density of adipose-derived mesenchymal stem cells in the culture medium is 1-2 x 105one/mL.
8. The culture method for inducing secretion of adipose-derived mesenchymal stem cell factor according to claim 6, wherein the induction culture process is performed at 37 ℃ and 5% CO2Under the conditions of (1).
9. The culture method for inducing secretion of adipose-derived mesenchymal stem cell factors according to claim 6, wherein the adipose-derived mesenchymal stem cells are subcultured to P3-P5 generation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011487212.9A CN112481206A (en) | 2020-12-16 | 2020-12-16 | Culture medium and culture method for inducing secretion of adipose-derived mesenchymal stem cell factor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011487212.9A CN112481206A (en) | 2020-12-16 | 2020-12-16 | Culture medium and culture method for inducing secretion of adipose-derived mesenchymal stem cell factor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112481206A true CN112481206A (en) | 2021-03-12 |
Family
ID=74917298
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011487212.9A Withdrawn CN112481206A (en) | 2020-12-16 | 2020-12-16 | Culture medium and culture method for inducing secretion of adipose-derived mesenchymal stem cell factor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112481206A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114438025A (en) * | 2022-02-22 | 2022-05-06 | 辛志远 | Induction medium and culture method for preparing adipose-derived mesenchymal stem cell factor |
| CN116970559A (en) * | 2023-09-21 | 2023-10-31 | 派能生物科技（深圳）有限公司 | Induction medium for preparing adipose-derived mesenchymal stem cell factor and application thereof |
-
2020
- 2020-12-16 CN CN202011487212.9A patent/CN112481206A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114438025A (en) * | 2022-02-22 | 2022-05-06 | 辛志远 | Induction medium and culture method for preparing adipose-derived mesenchymal stem cell factor |
| CN114438025B (en) * | 2022-02-22 | 2024-01-12 | 深圳市华晨阳科技有限公司 | Induction medium for preparing adipose-derived mesenchymal stem cell factor and culture method |
| CN116970559A (en) * | 2023-09-21 | 2023-10-31 | 派能生物科技（深圳）有限公司 | Induction medium for preparing adipose-derived mesenchymal stem cell factor and application thereof |
| CN116970559B (en) * | 2023-09-21 | 2023-11-28 | 派能生物科技（深圳）有限公司 | Induction medium for preparing adipose-derived mesenchymal stem cell factor and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112322580B (en) | Application of serum-free medium for mesenchymal stem cells | |
| CN106754639B (en) | Large-scale preparation method of mesenchymal stem cell factor | |
| CN106754668B (en) | Stem cell culture solution and injection | |
| KR101639988B1 (en) | Method for preparation of mesenchymal stem cell culture comprising high concentration of cell growth factors and composition prepared therefrom | |
| CN109627315B (en) | Adipose-derived mesenchymal stem cell factor freeze-dried powder and preparation method thereof | |
| JP5035737B2 (en) | Cell preparation for treating prostate cancer containing adipose tissue-derived mesenchymal stem cells | |
| CN112481206A (en) | Culture medium and culture method for inducing secretion of adipose-derived mesenchymal stem cell factor | |
| CN111621476B (en) | Serum-free culture medium for mesenchymal stem cells and preparation method thereof | |
| CN107267453A (en) | A kind of culture medium and its application for being used to cultivate fat mesenchymal stem cell | |
| CN105663168A (en) | Cell preparation for repairing ovarian functions | |
| CN106566803A (en) | Culture solution, application of culture solution and method for culturing umbilical cord mesenchymal stem cells | |
| CN110804607B (en) | Preparation method of high-concentration human mesenchymal stem cell lysate | |
| CN113151165B (en) | Culture medium and culture method for human umbilical cord mesenchymal stem cell amplification | |
| KR20150029280A (en) | Autologous and allogenic adipose tissue-derived mesenchymal stem cells composition for treatment of diabetic wound | |
| CN110935010A (en) | Stem cell preparation, growth factor composition, preparation method and application thereof | |
| KR20210145705A (en) | Mesenchymal stem cell culture media for preventing or treating inflammatory disease and a method for preparing the same | |
| CN112592892A (en) | Culture medium and culture method for inducing secretion of umbilical cord mesenchymal stem cell factors | |
| CN108373995B (en) | Stem cell conditioned medium, preparation method and application thereof | |
| CN111484973B (en) | Purification method of adipose-derived stem cells | |
| CN106978395B (en) | Method for efficiently separating and culturing umbilical cord mesenchymal stem cells | |
| CN106754650B (en) | A kind of endothelial progenitor cells cultural method of derived from bone marrow | |
| CN111454896B (en) | Induction method for improving differentiation capacity of mesenchymal stem cells for promoting acute myeloid leukemia | |
| CN106606512B (en) | Mixed cell preparation for treating myocardial infarction and preparation method and application thereof | |
| CN108588017A (en) | A kind of amplification method of umbilical cord mesenchymal stem cells and its application in arthritis | |
| CN109852584B (en) | Composition with effect of promoting mesenchymal stem cells to secrete cytokines and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20210312 |
|
| WW01 | Invention patent application withdrawn after publication |