CN112592827B - Transgenic culture dish for biological health research - Google Patents
Transgenic culture dish for biological health research Download PDFInfo
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- CN112592827B CN112592827B CN202110017145.2A CN202110017145A CN112592827B CN 112592827 B CN112592827 B CN 112592827B CN 202110017145 A CN202110017145 A CN 202110017145A CN 112592827 B CN112592827 B CN 112592827B
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- 238000011160 research Methods 0.000 title claims abstract description 19
- 230000009261 transgenic effect Effects 0.000 title claims abstract description 16
- 238000000926 separation method Methods 0.000 claims abstract description 71
- 238000004113 cell culture Methods 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 55
- 238000013519 translation Methods 0.000 claims description 30
- 238000007789 sealing Methods 0.000 claims description 16
- 230000005540 biological transmission Effects 0.000 claims description 14
- 239000011521 glass Substances 0.000 claims description 13
- 239000011159 matrix material Substances 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 9
- 230000000149 penetrating effect Effects 0.000 claims description 8
- 238000005192 partition Methods 0.000 claims description 4
- 239000013013 elastic material Substances 0.000 claims description 3
- 238000005086 pumping Methods 0.000 claims description 3
- 239000005394 sealing glass Substances 0.000 claims description 3
- 230000006870 function Effects 0.000 claims 2
- 230000009711 regulatory function Effects 0.000 claims 2
- 241000233866 Fungi Species 0.000 claims 1
- 238000012258 culturing Methods 0.000 abstract description 2
- 238000001514 detection method Methods 0.000 abstract description 2
- 238000000605 extraction Methods 0.000 abstract description 2
- 230000003204 osmotic effect Effects 0.000 abstract description 2
- 238000011002 quantification Methods 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 7
- 238000010586 diagram Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 238000004891 communication Methods 0.000 description 3
- 239000012531 culture fluid Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
Abstract
The invention discloses a transgenic culture dish for biological health research, which comprises a culture dish main body, wherein a working cavity is arranged in the culture dish main body, a culture plate is fixedly arranged on the inner lower wall of the working cavity, a culture cavity with an upward opening is arranged in the culture plate, the culture cavity with the upward opening is arranged in the culture cavity and used for carrying out cell culture, and a separation lifting cavity is communicated with the inner lower wall of the culture cavity; the invention carries out uniform cell culture in a large environment, facilitates the experimenter not to repeatedly carry out culture modulation for many times, carries out large environment separation after culturing biological research cells in a uniform environment for a period of time, further forms different small environments for further cell culture, judges the flora area of the cells in each separation cavity through color detection, controls the concentration of culture solution in each separation cavity to reach the cell osmotic concentration through extraction or input, and achieves quantification and improves the efficiency of the whole experimental research through control.
Description
Technical Field
The invention relates to the field of transgenic culture dishes, in particular to a transgenic culture dish for biological health research.
Background
In our daily life, the culture dish is a laboratory dish used for microorganism or cell culture, general culture dishes are all plane discs and have small volume, when laboratory personnel carry out cell culture, multiple times of separated culture are needed, so that the cell culture can reach the same culture environment, high-precision treatment is needed, the laboratory personnel are quite inconvenient, the concentration of a culture solution of cells needs to be paid attention, and the laboratory personnel are at the same culture temperature, so that the working requirement on the laboratory personnel is higher.
Disclosure of Invention
The invention aims to solve the technical problem of providing a transgenic culture dish for biological health research, and solves the problems of troublesome cell separation culture and difficult adjustment of the concentration of a culture solution.
The invention is realized by the following technical scheme.
The invention relates to a transgenic culture dish for biological health research, which comprises a culture dish main body, wherein a working cavity is arranged in the culture dish main body, a culture plate is fixedly arranged on the lower wall in the working cavity, a culture cavity with an upward opening is arranged in the culture plate, the culture cavity with the upward opening is arranged in the culture cavity and used for cell culture, a separation lifting cavity is communicated with the lower wall in the culture cavity, a separation plate capable of moving up and down is arranged in the separation lifting cavity, the outer end surface of the separation plate is attached to the inner wall of the culture cavity, an annular matrix is arranged in the separation plate, the separation cavity is communicated with the separation cavity in an up-and-down penetrating manner, a separation connecting cavity is communicated with the lower side in the culture cavity, a separation connecting plate capable of moving up and down is arranged in the separation connecting cavity, the left end surface of the separation connecting plate is fixedly connected with the lower side of the right end surface of the separation plate, the lower side of the left wall of the culture cavity is communicated with a vibration transmission cavity, the device is characterized in that a vibration meshing plate capable of moving left and right is arranged on the right side in the vibration transmission cavity, a vibration rotating shaft is rotatably arranged between the upper wall and the lower wall in the vibration transmission cavity, a vibration wheel is fixedly connected to the outer circular surface of the vibration rotating shaft, the rotation center of the vibration wheel is deviated from the rotation center of the vibration rotating shaft, the right side of the outer circular surface of the vibration wheel abuts against the left end surface of the vibration meshing plate, vibration springs which are symmetrical front and back with respect to the vibration rotating shaft are fixedly connected between the left end surface of the vibration meshing plate and the left wall of the vibration transmission cavity, a lifting sealing plate capable of moving up and down is arranged in the working cavity and positioned on the upper side of the culture plate, lifting sealing cavities which are distributed in an annular matrix arrangement and are communicated with each other in a vertical penetrating manner are arranged in the lifting sealing cavities, sealing elastic plates which can move up and down and are made of elastic materials are arranged in the lifting sealing elastic plates, and the sealing elastic plates are provided with upper and lower transparent sealing glass plates, the middle positions in all the closed glass plates are provided with connecting pipelines which are communicated in a penetrating way up and down, the upper ends in the connecting pipelines are fixedly provided with connecting valves, an adjusting function board which can move up and down is arranged in the working cavity and above the lifting sealing board, a moving cavity with a downward opening is arranged in the adjusting function board, a front and back moving board capable of moving front and back is arranged in the moving cavity, a translation cavity with a downward opening is arranged in the front and back moving plate, a moving working plate capable of moving left and right is arranged in the translation cavity, a lifting working cavity with a downward opening is arranged in the movable working plate, a supporting lifting plate capable of moving up and down is arranged in the lifting working cavity, the supporting lifting plate is fixedly provided with color detectors for detecting floras on the left side and the right side, and a connecting water gun for adjusting the concentration of a culture solution is fixedly arranged in the supporting lifting plate.
Preferably, it is equipped with the partition screw thread axle to separate to rotate between two walls about separating the connection intracavity, separate the screw thread axle with separate connecting plate threaded connection, it is equipped with the partition motor to separate connection intracavity lower wall internal fixation, separate screw thread axle lower extreme power connect in separate the motor, it is equipped with the pulley chamber to separate the motor downside, the rotation of the right wall of pulley intracavity is equipped with driving pulley, driving pulley up end axle center power connect in separate the motor, pulley intracavity left side wall rotates and is equipped with driven pulley, driven pulley with around being equipped with driving belt between the driving pulley, vibrations pivot lower extreme downwardly extending to the pulley chamber and with driven pulley up end axle center fixed connection.
Preferably, the right wall of the working chamber is communicated with two lifting connection chambers which are arranged in an upper matrix and a lower matrix and have the same size, the two lifting connection chambers are arranged on the upper side and the lower side of the right wall of the working chamber, the same lifting rotating shaft is arranged between the two walls of the lifting connection chamber in a penetrating manner, the outer circular surface of the lifting rotating shaft is located in the lifting connection chamber, lifting threaded sleeves with the same threads are fixedly arranged in the lifting connection chamber, the two lifting connection plates capable of moving up and down are arranged in the lifting connection chamber respectively on the upper side and the lower side, the lifting connection plates are in threaded connection with the lifting threaded sleeves, the right end surface and the lower side of the lifting connection plate are fixedly connected with the left end surface of the lifting connection plate, the right end surface and the upper side of the lifting connection plate are fixedly connected with the left end surface of the lifting connection plate, the lower wall of the lifting connection chamber is fixedly provided with a lifting motor, and the lower end of the lifting rotating shaft is dynamically connected with the lifting motor.
Preferably, the upper side of the left wall of the working cavity is communicated with a lifting support cavity, a lifting support rod is fixedly arranged on the upper wall and the lower wall of the lifting support cavity, a support plate capable of moving up and down is arranged in the lifting support cavity, the support plate is connected with the lifting support rod in a sliding mode, and the right end face of the support plate is fixedly connected with the left end face of the adjusting function plate.
Preferably, all the left and right walls of the lifting closed cavity are communicated with connecting cavities which are bilaterally symmetrical about the closed glass plate, lifting connecting sliders capable of moving up and down are arranged in the connecting cavities on the left and right sides, a connecting spring is fixedly connected between the lower end face of each lifting connecting slider and the lower end face of each connecting cavity, and the left and right end faces of the closed elastic plate are fixedly connected with the end face of one side, close to the closed glass plate, of each lifting connecting slider.
Preferably, it is equipped with translation threaded spindle to rotate between the two walls about the translation chamber, remove the work board with translation threaded spindle threaded connection, translation chamber right side wall internal fixation is equipped with translation motor, translation threaded spindle right-hand member power connect in translation motor, it is equipped with the connection water pipe to connect squirt upper end rear side intercommunication, lift work intracavity back wall intercommunication is equipped with the regulation and connects the chamber, it is equipped with the adjusting connection board that can reciprocate to adjust the connection intracavity, adjusting connection board preceding terminal surface with support lifter rear end face fixed connection, it is equipped with the regulation threaded spindle to rotate between the two walls about the regulation connection intracavity, adjust the threaded spindle with adjusting connection board threaded connection, it is equipped with adjusting motor to adjust connection intracavity internal fixation, adjust threaded spindle upper end power connect in adjusting motor.
Preferably, a movable threaded shaft is rotatably arranged on the left side between the front wall and the rear wall of the movable cavity and is in threaded connection with the left end of the front and rear movable plate, a movable motor is fixedly arranged in the rear wall of the movable cavity, and the rear end of the movable threaded shaft is in power connection with the movable motor.
Preferably, be equipped with the liquid storage water tank in the removal chamber upper wall, liquid storage water tank right side wall internal fixation is equipped with the water pump motor, the fixed water pump that is equipped with in water pump motor right side, water pump left end power connect in the water pump motor, water pump upper end intercommunication is equipped with outlet conduit, outlet conduit with it is equipped with telescopic pipeline to connect the intercommunication between the water pipe, the water pump lower extreme with it is equipped with drinking-water pipeline to communicate between the liquid storage water tank right side wall downside.
The invention has the beneficial effects that: the invention carries out uniform cell culture in a large environment, facilitates the experimenter not to repeatedly carry out culture modulation for many times, carries out large environment separation after culturing biological research cells in a uniform environment for a period of time, further forms different small environments for further cell culture, judges the flora area of the cells in each separation cavity through color detection, controls the concentration of culture solution in each separation cavity to reach the cell osmotic concentration through extraction or input, and achieves quantification and improves the efficiency of the whole experimental research through control.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the invention, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an embodiment of the present invention;
FIG. 2 is a schematic structural diagram of A-A in FIG. 1 according to an embodiment of the present invention;
FIG. 3 is a schematic structural diagram of B-B in FIG. 1 according to an embodiment of the present invention;
FIG. 4 is a schematic structural diagram of C-C in FIG. 3 according to an embodiment of the present invention;
FIG. 5 is an enlarged schematic view of the embodiment of the present invention at D in FIG. 1;
FIG. 6 is a schematic structural diagram of E-E in FIG. 5 according to an embodiment of the present invention;
FIG. 7 is a schematic structural diagram of F-F in FIG. 1 according to an embodiment of the present invention.
Detailed Description
The invention will now be described in detail with reference to fig. 1-7, wherein for ease of description the orientations described below are now defined as follows: the up, down, left, right, and front-back directions described below correspond to the up, down, left, right, and front-back directions in the projection relationship of fig. 1 itself.
With reference to fig. 1-7, a transgenic culture dish for biological health research comprises a culture dish main body 11, a working chamber 22 is provided in the culture dish main body 11, a culture plate 27 is fixedly provided at the inner lower wall of the working chamber 22, a culture chamber 28 with an upward opening is provided in the culture plate 27, the culture chamber 28 with the upward opening is provided in the culture chamber 28 for cell culture, a separation lifting chamber 64 is provided in the culture chamber 28 in communication with the inner lower wall thereof, a separation plate 36 capable of moving up and down is provided in the separation lifting chamber 64, the outer end surface of the separation plate 36 is attached to the inner wall of the culture chamber 28, a separation chamber 67 in communication with the separation lifting chamber 36 in a vertical penetrating manner is provided in an annular matrix, a separation connection chamber 38 is provided in communication with the inner lower side of the culture chamber 28, a separation connection plate 41 capable of moving up and down is provided in the separation connection chamber 38, the left end surface of the separation connection plate 41 is fixedly connected to the lower side of the right end surface of the separation plate 36, the culture chamber 28 is provided with a vibration transmission chamber 30 communicated with the lower side of the left wall, a vibration meshing plate 32 capable of moving left and right is arranged on the right side in the vibration transmission chamber 30, a vibration rotating shaft 29 is arranged between the upper wall and the lower wall in the vibration transmission chamber 30 in a rotating manner, a vibration wheel 31 is fixedly connected with the outer circular surface of the vibration rotating shaft 29, the rotating center of the vibration wheel 31 is deviated from the rotating center of the vibration rotating shaft 29, the right side of the outer circular surface of the vibration wheel 31 is abutted against the left end surface of the vibration meshing plate 32, a vibration spring 47 which is symmetrical with respect to the front and back of the vibration rotating shaft 29 is fixedly connected between the left end surface of the vibration meshing plate 32 and the left wall of the vibration transmission chamber 30, a lifting sealing plate 26 capable of moving up and down is arranged in the working chamber 22 and positioned on the upper side of the culture plate 27, and a lifting sealing chamber 68 which is distributed in an annular matrix arrangement and is communicated with the lifting sealing plate 26 up and down is arranged, a closed elastic plate 48 which can move up and down and is made of elastic materials is arranged in all the lifting closed cavities 68, a transparent closed glass plate 49 is arranged in the closed elastic plate 48, a connecting pipeline 51 which is communicated with the closed glass plate 49 in a penetrating manner is arranged at the middle position in the closed glass plate 49, a connecting valve 50 is fixedly arranged at the upper end in the connecting pipeline 51, an adjusting function plate 63 which can move up and down is arranged in the working cavity 22 and above the lifting closed plate 26, a moving cavity 66 with a downward opening is arranged in the adjusting function plate 63, a front and back moving plate 19 which can move front and back is arranged in the moving cavity 66, a translation cavity 21 with a downward opening is arranged in the front and back moving plate 19, a moving working plate 53 which can move left and right is arranged in the translation cavity 21, a lifting working cavity 69 with a downward opening is arranged in the moving working plate 53, and a supporting lifting plate 57 which can move up and down is arranged in the lifting working cavity 69, the color detector 54 for detecting flora is fixedly arranged on the left side and the right side of the supporting lifting plate 57, and the connecting water gun 55 for adjusting the concentration of the culture solution is fixedly arranged in the supporting lifting plate 57.
Beneficially, separation threaded shaft 37 is rotatably arranged between the upper wall and the lower wall in separation connecting cavity 38, separation threaded shaft 37 is in threaded connection with separation connecting plate 41, separation motor 39 is fixedly arranged in the lower wall of separation connecting cavity 38, the lower end of separation threaded shaft 37 is in power connection with separation motor 39, pulley cavity 35 is arranged on the lower side of separation motor 39, driving pulley 40 is rotatably arranged on the right wall in pulley cavity 35, the upper end face of driving pulley 40 is in axial force connection with separation motor 39, driven pulley 33 is rotatably arranged on the left wall in pulley cavity 35, driving belt 34 is wound between driven pulley 33 and driving pulley 40, and lower end face of vibration rotating shaft 29 extends downwards to pulley cavity 35 and is fixedly connected with the upper end face of driven pulley 33.
Advantageously, the right wall of the working chamber 22 is provided with two lifting connecting chambers 44 which are arranged in an up-down matrix and have the same size, the same lifting rotating shaft 12 is arranged between the upper wall and the lower wall of the lifting connecting chamber 44 on the upper side and the lower side in a penetrating and rotating manner, a lifting threaded sleeve 45 with the same thread is fixedly arranged on the outer circular surface of the lifting rotating shaft 12 and positioned in the lifting connecting cavity 44, two lifting connecting plates 46 capable of moving up and down are respectively arranged in the lifting connecting cavities 44 on the upper side and the lower side, the lifting connecting plate 46 is in threaded connection with the lifting threaded sleeve 45, the right end face of the lifting closing plate 26 is fixedly connected with the left end face of the lifting connecting plate 46 at the lower side, the right end face of the adjusting function plate 63 is fixedly connected with the left end face of the upper lifting connecting plate 46, a lifting motor 42 is fixedly arranged in the lower wall of the lifting connecting cavity 44 at the lower side, and the lower end of the lifting rotating shaft 12 is in power connection with the lifting motor 42.
Beneficially, the upper side of the left wall of the working cavity 22 is communicated with a lifting support cavity 24, a lifting support rod 25 is fixedly arranged on the upper wall and the lower wall in the lifting support cavity 24, a support plate 23 capable of moving up and down is arranged in the lifting support cavity 24, the support plate 23 is slidably connected with the lifting support rod 25, and the right end face of the support plate 23 is fixedly connected with the left end face of the adjusting function plate 63.
Beneficially, the left and right walls of all the lifting closed cavities 68 are communicated with connection cavities 72 which are bilaterally symmetrical with respect to the closed glass plate 49, lifting connection sliders 52 which can move up and down are arranged in the connection cavities 72 on the left and right sides, a connection spring 71 is fixedly connected between the lower end face of each lifting connection slider 52 and the lower end face of each connection cavity 72, and the left and right end faces of the closed elastic plate 48 are fixedly connected with the end face of each lifting connection slider 52 on the side close to the closed glass plate 49.
Advantageously, a translation threaded shaft 20 is rotatably arranged between the left wall and the right wall of the translation cavity 21, the movable working plate 53 is in threaded connection with the translation threaded shaft 20, a translation motor 43 is fixedly arranged in the right wall of the translation cavity 21, the right end of the translation threaded shaft 20 is dynamically connected with the translation motor 43, the rear side of the upper end of the connecting water gun 55 is communicated with a connecting water pipe 56, the rear wall in the lifting working cavity 69 is communicated with an adjusting connecting cavity 62, an adjusting connecting plate 61 capable of moving up and down is arranged in the adjusting connecting cavity 62, the front end surface of the adjusting connecting plate 61 is fixedly connected with the rear end surface of the supporting lifting plate 57, an adjusting threaded shaft 60 is rotatably arranged between the upper wall and the lower wall in the adjusting connecting cavity 62, the adjusting threaded shaft 60 is in threaded connection with the adjusting connecting plate 61, an adjusting motor 59 is fixedly arranged in the upper wall of the adjusting connecting cavity 62, and the upper end of the adjusting threaded shaft 60 is in power connection with the adjusting motor 59.
Advantageously, a moving threaded shaft 70 is rotatably arranged at the left side between the front wall and the rear wall in the moving cavity 66, the moving threaded shaft 70 is in threaded connection with the left end of the front and rear moving plate 19, a moving motor 65 is fixedly arranged in the rear wall of the moving cavity 66, and the rear end of the moving threaded shaft 70 is in power connection with the moving motor 65.
Beneficially, a liquid storage water tank 18 is arranged in the upper wall of the moving cavity 66, a water pump motor 17 is fixedly arranged in the right wall of the liquid storage water tank 18, a water pump 13 is fixedly arranged on the right side of the water pump motor 17, the left end of the water pump 13 is in power connection with the water pump motor 17, an upper end of the water pump 13 is communicated with a water outlet pipeline 14, a telescopic pipeline 58 is communicated between the water outlet pipeline 14 and the connecting water pipe 56, and a water pumping pipeline 16 is communicated between the lower end of the water pump 13 and the lower side of the right wall of the liquid storage water tank 18.
In the initial state, the lifting closing plate 26 is located at the upper limit position, the separation plate 36 is located at the lower limit position, the lifting connecting slide block 52 is located at the upper limit position, the supporting lifting plate 57 is located at the upper limit position, the adjusting connecting plate 61 is located at the upper limit position, and the adjusting function plate 63 is located at the upper limit position.
During working, cultured cells and culture solution which need to be studied on biological health are placed in the culture cavity 28 for primary and same culture, when the cells generate floras with different sizes after being cultured in the same environment for a period of time, the separation motor 39 is started to drive the separation threaded shaft 37 to rotate, the separation threaded shaft 37 drives the separation connecting plate 41 to move upwards through gear engagement, the separation connecting plate 41 drives the separation plate 36 to move upwards through fixed connection, the cell culture solution in the culture cavity 28 is separated in equal amount and finally separated in each separation cavity 67 through the separation cavities 67 which are distributed in a matrix arrangement, in the engineering, the separation motor 39 drives the driving pulley 40 to rotate, the driving pulley 40 drives the driven pulley 33 to rotate through the transmission belt 34, the driven pulley 33 drives the vibration wheel 31 to rotate through the vibration rotating shaft 29, at this time, because the rotation center of the vibration wheel 31 deviates from the rotation center of the vibration rotating shaft 29, the vibration wheel 31 eccentrically rotates and pushes the right vibration engaging plate 32 to move rightwards, then the vibration engaging plate 32 moves leftwards due to the elastic force of the vibration spring 47, the effect of reciprocating left and right vibration is achieved, the vibration is transmitted to the separating plate 36 by the vibration engaging plate 32 abutting against the left end surface of the separating plate 36, when the separating plate 36 ascends to separate the culture solution in the culture chamber 28, the cell flora adhered to the upper end surface of the separating plate 36 is slightly vibrated down and all the flora can enter different separating chambers 67, at the same time, the lifting motor 42 rotates to drive the lifting rotating shaft 12 to rotate, at this time, the lifting rotating shaft 12 drives the lifting threaded sleeves 45 at the upper and lower sides, at this time, the lifting threaded sleeves 45 at the upper and lower sides drive the lifting connecting plate 46 to move downwards through threaded connection, at this time, the lifting connecting plate 46 at the lower side drives the lifting closing plate 26 to move downwards, at the moment, the lifting closing plate 26 moves downwards to drive the closing elastic plates 48 to move downwards and correspondingly and abuttingly contact with the upper sides of all the separation cavities 67 through all the closing elastic plates 48, then, in the process that the lifting closing plate 26 moves to the lower limit position, the closing elastic plates 48 move downwards and close the upper sides of the separation cavities 67 through the closing glass plates 49, at the moment, the translation motor 43 rotates to drive the translation threaded shaft 20 to rotate, at the moment, the translation threaded shaft 20 drives the moving working plate 53 to move left and right through threaded connection and drives the moving threaded shaft 70 to rotate through the moving motor 65, and drives the front and back moving plate 19 to move back and forth through threaded connection, so that the effect of controlling the moving working plate 53 to move left and right is achieved, then, when the moving working plate 53 sequentially moves above each closing glass plate 49, the transparent closing glass plates 49 penetrate through the color detector 54 to detect the number of florae in each separation cavity 67, at the moment, the adjusting motor 59 is started to drive the adjusting threaded shaft 60 to rotate forwards, at the moment, the adjusting threaded shaft 60 rotates to drive the adjusting connecting plate 61 to move downwards through threaded connection, and the adjusting connecting plate 61 is fixedly connected with the supporting lifting plate 57 to drive the supporting lifting plate 57 to move downwards, at the moment, the supporting lifting plate 57 drives the connecting water gun 55 to move downwards and enable the connecting water gun 55 to be communicated with the connecting valve 50, at the moment, after the number of florae is detected through the color detector 54, if the area of the florae separated in the separation cavity 67 is small at the moment, the color detector 54 controls the water pump motor 17 to start in a reverse rotation mode, at the moment, the water pump 13 starts in a reverse rotation mode to drive the telescopic pipeline 58 connected through the water outlet pipeline 14, and the culture fluid in the corresponding separation cavity 67 is pumped up through the connecting pipeline 51 connected with the connecting water gun 55 connected with the connecting water pipe 56 and is input into the fluid storage tank 18 for storage, so that the effect of adjusting the concentration of the culture fluid in the separation cavity 67 according to the number of the florae is achieved, if the color detector 54 detects that the area of the separated flora in the separation cavity 67 is large, the water pump motor 17 rotates forward to pump the culture solution stored in the solution storage tank 18 through the water pumping pipeline 16 and output the culture solution upwards to the water outlet pipeline 14, and the culture solution is input into the separation cavity 67 through the connecting pipeline 51 to increase the concentration of the culture solution, so that the effect of controlling the concentration of the culture solution in the separation cavity 67 is achieved.
The above embodiments are merely illustrative of the technical ideas and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.
Claims (8)
1. The utility model provides a because biological health research uses transgenic culture dish, includes the culture dish main part, its characterized in that: the culture dish is characterized in that a working cavity is arranged in the culture dish main body, a culture plate is fixedly arranged on the inner lower wall of the working cavity, a culture cavity with an upward opening is arranged in the culture plate, the culture cavity with the upward opening is arranged in the culture cavity and used for cell culture, a separation lifting cavity is communicated with the inner lower wall of the culture cavity and provided with a separation plate capable of moving up and down, the separation lifting cavity is internally provided with a separation plate, the outer end surface of the separation plate is attached to the inner wall of the culture cavity, an annular matrix is arranged in the separation plate and penetrates through the separation cavity from top to bottom, a separation connecting cavity is communicated with the lower side of the culture cavity and provided with a separation connecting plate capable of moving up and down, the left end surface of the separation connecting plate is fixedly connected with the lower side of the right end surface of the separation plate, a vibration transmission cavity is communicated with the lower side of the left wall of the culture cavity, and a vibration meshing plate capable of moving left and right is arranged on the right side of the vibration transmission cavity, the vibration transmission device is characterized in that a vibration rotating shaft is rotatably arranged between an upper wall and a lower wall in the vibration transmission cavity, a vibration wheel is fixedly connected to the outer circular surface of the vibration rotating shaft, the rotation center of the vibration wheel is deviated from the rotation center of the vibration rotating shaft, the right side of the outer circular surface of the vibration wheel abuts against the left end surface of the vibration meshing plate, vibration springs which are symmetrical front and back of the vibration rotating shaft are fixedly connected between the left end surface of the vibration meshing plate and the left wall of the vibration transmission cavity, a lifting sealing plate which can move up and down is arranged in the working cavity and positioned on the upper side of the culture plate, lifting sealing cavities which are distributed in an annular matrix arrangement mode and are communicated up and down are arranged in the lifting sealing plate, sealing elastic plates which can move up and down and are made of elastic materials are arranged in the lifting sealing cavities, upper and lower transparent sealing glass plates are arranged in the sealing elastic plates, and connecting pipelines which are communicated up and down are arranged in the middle positions in the sealing glass plates, the upper end is fixed and is equipped with the connecting valve in the connecting tube, the work intracavity just is located lift shut top is equipped with the regulatory function board that can reciprocate, be equipped with the decurrent chamber that removes of opening in the regulatory function board, it is equipped with the movable plate around that can the back-and-forth movement to remove the intracavity, be equipped with the translation chamber that opens downwardly in the movable plate around, the translation intracavity is equipped with the removal work board that can remove about, be equipped with the decurrent lift work chamber of opening in the removal work board, the lift work intracavity is equipped with the support lifter plate that can reciprocate, the fixed colour detector that is used for carrying on the fungus crowd and detects that is equipped with in the left and right sides of support lifter plate, the support lifter plate internal fixation is equipped with the connection squirt and is used for adjusting culture solution concentration.
2. The transgenic culture dish for biological health research according to claim 1, wherein: it is equipped with the partition screw thread axle to separate to rotate between two walls about separating the connection intracavity, separate the screw thread axle with separate connecting plate threaded connection, it is equipped with the partition motor to separate connection intracavity lower wall internal fixation, separate screw thread axle lower extreme power connect in separate the motor, it is equipped with the band pulley chamber to separate the motor downside, the rotation of band pulley intracavity right side wall is equipped with driving pulley, driving pulley up end axle center power connect in separate the motor, the rotation of band pulley intracavity left side wall is equipped with driven pulleys, driven pulleys with around being equipped with driving belt between the driving pulley, vibrations pivot lower extreme downwardly extending to the band pulley chamber and with driven pulleys up end axle center fixed connection.
3. The transgenic culture dish for biological health research according to claim 1, wherein: the lifting connection cavity is internally provided with two lifting connection cavities, wherein the two lifting connection cavities are arranged in a matrix manner and have the same size, the two lifting connection cavities are arranged on the right wall of the working cavity in a communicated manner, the upper side and the lower side of the working cavity are connected with the upper wall and the lower wall of the lifting connection cavity in a penetrating manner and are provided with the same lifting rotating shaft, the outer circular surface of the lifting rotating shaft is positioned in the lifting connection cavity, lifting threaded sleeves with the same threads are fixedly arranged in the lifting connection cavity, the upper side and the lower side of the lifting connection cavity are respectively provided with two lifting connection plates capable of moving up and down, the lifting connection plates are in threaded connection with the lifting threaded sleeves, the right end surface and the lower side of the lifting connection plates are fixedly connected with the left end surface of the lifting connection plates, the right end surface and the upper side of the lifting connection plates are fixedly connected with the left end surface of the lifting connection plates, the lower end of the lifting connection cavity is fixedly connected with the lifting motor.
4. The transgenic culture dish for biological health research according to claim 1, wherein: the utility model discloses a lift support of adjusting function board, including work chamber, lift bracing piece, backup pad, lifting support intracavity, the backup pad that can reciprocate is equipped with the lift bracing piece, the backup pad with lifting support piece sliding connection, the backup pad right-hand member face with adjust function board left end face fixed connection.
5. The transgenic culture dish for biological health research according to claim 1, wherein: all the left and right walls of the lifting sealed cavity are communicated with connecting cavities which are bilaterally symmetrical about the sealed glass plate, lifting connecting sliders capable of moving up and down are arranged in the connecting cavities on the left and right sides, connecting springs are fixedly connected between the lower end faces of the lifting connecting sliders and the lower wall end faces of the connecting cavities, and the left and right end faces of the sealed elastic plate are fixedly connected with the end face, close to one side of the sealed glass plate, of the lifting connecting sliders.
6. The transgenic culture dish for biological health research according to claim 1, wherein: rotate between the two walls about the translation chamber and be equipped with translation screw thread axle, remove the work board with translation screw thread axle threaded connection, translation chamber right side wall internal fixation is equipped with translation motor, translation screw thread axle right-hand member power connect in translation motor, it is equipped with the connection water pipe to connect squirt upper end rear side intercommunication, lift work intracavity back wall intercommunication is equipped with the regulation and connects the chamber, it is equipped with the adjusting connection board that can reciprocate to adjust the connection intracavity, adjusting connection board preceding terminal surface with support lifter plate rear end face fixed connection, it is equipped with the regulation screw thread axle to rotate between the two walls about adjusting the connection intracavity, adjust the screw thread axle with adjusting connection board threaded connection, it is equipped with adjusting motor to adjust the connection intracavity internal fixation, adjust screw thread axle upper end power connect in adjusting motor.
7. The transgenic culture dish for biological health research according to claim 1, wherein: the movable threaded shaft is rotatably arranged on the left side between the front wall and the rear wall of the movable cavity and is in threaded connection with the left end of the front movable plate and the rear movable plate, a movable motor is fixedly arranged in the rear wall of the movable cavity, and the rear end of the movable threaded shaft is in power connection with the movable motor.
8. The transgenic culture dish for biological health research according to claim 7, wherein: the movable water pump is characterized in that a liquid storage water tank is arranged in the upper wall of the movable cavity, a water pump motor is fixedly arranged in the right wall of the liquid storage water tank, a water pump is fixedly arranged on the right side of the water pump motor, the left end of the water pump is in power connection with the water pump motor, a water outlet pipeline is communicated with the upper end of the water pump and provided with a telescopic pipeline in a communicated mode between the water outlet pipelines, and a water pumping pipeline is communicated between the lower end of the water pump and the lower side of the right wall of the liquid storage water tank.
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