CN112586503A

CN112586503A - Cyazofamid-containing bactericidal composition and application thereof

Info

Publication number: CN112586503A
Application number: CN202011530290.2A
Authority: CN
Inventors: 詹敏敏; 贾志新; 冯亚东; 严强; 杨的妹; 柴明根; 马超; 欧阳灿; 王光瀛
Original assignee: JIANGXI HEYI CHEMICALS CO Ltd
Current assignee: JIANGXI HEYI CHEMICALS CO Ltd
Priority date: 2020-12-22
Filing date: 2020-12-22
Publication date: 2021-04-02

Abstract

The invention discloses a cyazofamid-containing bactericidal composition and application thereof, wherein the active ingredients are cyazofamid and metrafenone, the mass ratio of cyazofamid to metrafenone is 50:1-1:50, and the balance is auxiliary ingredients which are allowed to be used and can be accepted in pesticides. The bactericidal composition can be prepared into microemulsion, aqueous emulsion, suspending agent, wettable powder and water dispersible granules by a known method. The composition has obvious synergistic effect and can be used for preventing and treating powdery mildew on crops such as vegetables, melons and fruits.

Description

Cyazofamid-containing bactericidal composition and application thereof

Technical Field

The invention relates to a sterilization composition, in particular to a cyazofamid-containing sterilization composition and application thereof in preventing and treating diseases on crops, and belongs to the technical field of pesticides.

Background

Powdery mildew is a plant disease caused by the powdery mildew family of fungi. For example: powdery mildew of cereals caused by erysiphe graminis: powdery mildew of various plants such as melons, beans, hemp and the like caused by cocklebur unilamella: strawberry powdery mildew caused by the devil's claw shell of strawberry: powdery mildew of more than 80 trees such as mulberry, pear, persimmon, walnut, chestnut, lychee, papaya and the like caused by baseball coniferous shell: powdery mildew of rose flowers caused by rose monofilament shells, and the like.

Powdery mildew is common in energy-saving greenhouse vegetable cultivation, particularly extremely serious in damage to cucumber crops such as cucumbers, pumpkin, melons and the like, and also commonly occurs in tomatoes, eggplants and peppers and pimentos. Most vegetable growers reflect that the disease is difficult to prevent and cure, and as long as the disease occurs, the disease is extremely difficult to eliminate no matter what kind of pesticide is sprayed, and finally seedlings have to be pulled out.

In recent years, the resistance to powdery mildew is more and more serious due to single medication and unscientific medication, and the resistance becomes a great problem for chemical prevention and treatment.

The inventor tries to compound the existing sterilization active ingredients, but the effect achieved by simple combination is not satisfactory.

Disclosure of Invention

The object of the present invention is to provide a germicidal composition for solving at least one of the above problems.

In a first aspect: the invention provides a bactericidal composition containing cyazofamid and metrafenone, which comprises cyazofamid and metrafenone in a mass ratio of 50:1-1: 50.

Preferably, the mass ratio of cyazofamid to metrafenone is 20:1-1: 20.

Preferably, the total mass of the cyazofamid and the metrafenone accounts for 1-90% of the mass of the bactericidal composition.

Preferably, the bactericidal composition further comprises the following substances in percentage by mass: 1-2% of azoxystrobin.

Preferably, the bactericidal composition comprises the following substances in percentage by mass: cyazofamid 50%, metrafenone 40%, azoxystrobin 2% and bupirimate 6%.

Preferably, the bactericidal composition comprises the following substances in percentage by mass: cyazofamid 30%, metrafenone 65%, azoxystrobin 2% and bupirimate 3%.

Preferably, the dosage form of the bactericidal composition is one of microemulsion, emulsion in water, suspending agent, wettable powder and water dispersible granule.

Preferably, the following components: the bactericidal composition is used for powdery mildew on crops such as vegetables, melons and fruits.

In a second aspect, the invention provides a bactericide, which comprises, by mass, 25% of cyazofamid, 22.5% of metrafenone, 1% of azoxystrobin, 1.5% of bupirimate, 2% of sodium dodecyl sulfate (wetting agent), 5% of sodium lignosulfonate (dispersing agent), 5% of sodium alkyl naphthalene sulfonate (dispersing agent), 10% of ammonium sulfate (disintegrating agent) and 100% of kaolin (filler).

Cyazofamid, chemical name: 4-chloro-2-cyano-N, N-dimethyl-5-p-tolylimidazole-1-sulfonamide. Cyazofamid is a sulfaimidazole bactericide produced by Japan Shiyao Producens, a super-protective bactericide, and is a mitochondrial respiration inhibitor, has high inhibition effect on various growth stages of oomycetes, including sporangium formation, sporangium germination, oospore formation, zoospore release, zoospore movement and hypha growth, and has activity on pathogenic bacteria with resistance or sensitivity to metalaxyl. Has special effects on downy mildew, epidemic disease, clubroot, damping-off and the like, and is used for preventing and treating germs of potatoes, grapes, vegetables (cucumbers, cabbages, tomatoes, onions and lettuce) and lawns. No interactive drug resistance with other systemic bactericides; the medicament has low toxicity, good toxicological property and safety to ecological environment, crops and non-target organisms.

Metrafenone, the common name Metrafenone, was first a benzophenone-type fungicide by cyanamide corporation of the united states (now basf, germany). The bactericidal composition has lipophilicity, is easy to be absorbed by plants, has a novel mechanism of action mechanism, inhibits the growth of pathogenic bacteria by interfering the transmission and action of mobile protein of the pathogenic bacteria, has excellent activity on almost all growth stages of the pathogenic bacteria, can be used for preventing and treating powdery mildew on crops such as grains, melons, fruits, beans, grapes and the like, and has no cross resistance with other bactericides. But the single use is relatively high in price, and the long-term single use is not beneficial to resistance control.

Azoxystrobin, a Strobilurin esterBactericidal pesticideHigh efficiency, broad spectrum, for almost allKingdom fungi(Ascomycotina、BasidiomycotinaFlagellates, anddeuteromycotina) Diseases such as powdery mildew, rust disease, glume blight, net blotch,Downy mildew of fruit of downy mildewAnd the rice blast and the like have good activity. Can be used for stem leaf spraying, seed treatment, and soil treatment, and is mainly used for grain, rice, peanut, fertilizer,grapePotato, fruit trees, vegetables, coffee, lawn, etc. The dosage is 25 ml-50/mu.

Bupirimate: bupirimate is a toxic waxy solid with a melting point of 50-51 ℃ for preventing and treating powdery mildew of crops such as apples, greenhouse roses, strawberries and the like, and is light yellow or light brown, also known as 5-butyl-2-ethylamino-6-methylpyrimidin-4-yl dimethyl amino sulfate.

The adjuvants used in the fungicidal composition described in the present invention include wetting agents, dispersants, antifreezes, thickeners, penetrants, and the like, and other known substances useful for stabilization of active ingredients during storage and use and for exerting medicinal effects, are various ingredients commonly used or allowed to be used in pesticide preparations, and are not particularly limited, and the specific ingredients and amounts thereof are determined by simple experiments according to the formulation requirements.

The germicidal compositions described herein may be provided in a finished formulation, i.e., the materials in the composition are already mixed, or may be provided in a single dose combination, mixed directly in a tub or tank prior to use, and then diluted to the desired concentration.

The bactericidal composition is mainly used for preventing and treating diseases of crops such as vegetables, melons and fruits, and particularly powdery mildew.

Compared with the prior art, the bactericidal composition has the beneficial effects that: (1) the bactericidal composition has good synergistic effect in a certain range, and the control effect is obviously improved compared with that of a single agent; (2) after the pesticide effect is improved, the field dosage can be greatly reduced, the production and use cost is reduced, the pesticide residue is reduced, and the sustainable development of the environment is facilitated; (3) the effective components in the bactericidal composition have different action mechanisms, and are beneficial to overcoming and delaying the generation of drug resistance of pathogenic bacteria.

Detailed Description

The present invention is described in detail with reference to the following embodiments for the purpose of better understanding the objects, technical solutions and advantages of the present invention, but the present invention is not limited to these embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Different pesticide active ingredients are compounded, which is an effective way for solving the problems of pesticide effect, resistance and the like in the application process of a single pesticide at present. After different pesticide active ingredients are compounded, three action types, namely additive action, synergistic action and antagonistic action are usually shown, but the specific action cannot be predicted and can be known only through a large number of tests. The compound synergistic formula is good, can improve the actual prevention and treatment effect, reduces the using amount of pesticides, is beneficial to delaying the generation of resistance, and is an important means for scientifically preventing and treating diseases.

The bactericidal composition takes cyazofamid as one effective component and metrafenone as the other effective component, the combination of the cyazofamid and metrafenone has obvious synergistic effect on powdery mildew, and the combination of the cyazofamid and metrafenone is not only simple addition of two drug effects, but also is specifically illustrated by the following bioassay examples. Bioassay example 1. indoor toxicity assay test of cyazofamid and metrafenone combination for cucumber powdery mildew

Test subjects: powdery mildew of cucumber

The test was conducted by potting. The potted plants grow to 4-5 true leaves, and the number is reserved. And uniformly shaking off and inoculating powdery mildew fresh spores generated in 24 hours on the diseased cucumber leaves on the cucumber seedlings to be tested. According to the activity of the medicament, 5-7 series of mass concentrations are set. After inoculation for 24 hours, the liquid medicine is uniformly sprayed on the leaf surfaces until the leaf surfaces are completely wetted, and the liquid medicine is naturally dried for later use. Each treatment was repeated 4 times for 3 pots, and a blank was set for treatments containing only solvent and surfactant but no active ingredient. The inoculated test material is transferred to a climatic chamber (the relative humidity is 100 percent, the temperature is 20 +/-2 ℃) for culturing for 7 days. And (4) counting the disease spot area on each pot of cucumber leaves, investigating the disease index and calculating the prevention and treatment effect.

Grading standards

Level 0: the leaves have no disease spots;

level 1: the area of the lesion spots accounts for less than 5% of the area of the whole leaf;

and 3, level: the area of the lesion spots accounts for 6 to 10 percent of the area of the whole leaf;

and 5, stage: the area of the lesion spots accounts for 11-20% of the area of the whole leaf;

and 7, stage: the area of the lesion spots accounts for 21-50% of the area of the whole leaf;

and 9, stage: the area of the lesion spots accounts for more than 50 percent of the area of the whole leaf.

The drug effect calculation method comprises the following steps:

the preventing and treating effect is converted into several values (y), the concentration of the liquid medicine (mug/mL) is converted into a logarithmic value (x), and a virulence equation and an inhibitory intermediate concentration EC are calculated by a least square method₅₀Calculating out the toxicity of each medicine by the Sun Yunpei methodForce index and co-toxicity coefficient (CTC) of the combination.

Measured virulence index (ATI) ═ standard agent EC₅₀Reagent for test EC₅₀)×100

Theoretical virulence index (TTI) ═ A agent virulence index x percent of A in the mixture + B agent virulence index x percent of B in the mixture

Cotoxicity Coefficient (CTI) ═ [ actually measured virulence index (ATI)/theoretical virulence index (TTI) of mixture) ] × 100

When the CTC is less than or equal to 80, the composition shows antagonism, when 80< CTC is less than 120, the composition shows additive action, and when the CTC is more than or equal to 120, the composition shows synergistic action.

Indoor virulence assay results are shown in Table 1

TABLE 1 indoor toxicity test results of cyazofamid and metrafenone complex formulation for cucumber powdery mildew

Test results show that when the ratio of cyazofamid to metrafenone is 50:1-1:50, the co-toxicity coefficients are all over 120.0 and have a certain synergistic effect, and when the ratio is 10:1-1:10, the co-toxicity coefficients are all higher than 200.0 and the synergistic effect is more obvious.

The compound bactericidal composition can be prepared into microemulsion, emulsion in water, suspending agent, wettable powder and water dispersible granules suitable for agriculture by using a known method. The following specific examples are used to illustrate that the percentages in the formula are mass percentages, the effective content of cyazofamid is 94% and the effective content of metrafenone is 98%, and the effective contents are calculated according to the effective percentage when the cyazofamid and metrafenone are used, which is not described in detail below.

Microemulsion example

The effective components of the pesticide are dissolved in the solvent and the cosolvent, the surfactant is added, and the microemulsion is obtained after even mixing.

Example 1 microemulsion of 25% cyazofamid-metrafenone

Cyazofamid 20%, metrafenone 5%, dimethylbenzene (solvent) 9%, cyclohexanone (solvent) 9%, N-dimethylacetamide (solvent) 10%, calcium dodecylbenzene sulfonate (emulsifier) 4%, phenethylphenol polyoxyethylene method (emulsifier) 5%, castor oil polyoxyethylene ether (emulsifier) 5%, and deionized water to make up to 100%.

Examples of aqueous emulsions

Dissolving the effective components of the pesticide in a solvent and a cosolvent, adding a surfactant, and uniformly mixing to obtain an oil phase; mixing the water-soluble components, deionized water and other components to prepare a water phase, adding the oil phase into the water phase while stirring, and shearing at a high speed to form uniform emulsion to obtain the aqueous emulsion.

Example 2 aqueous 10% cyazofamid-metrafenone emulsion

5% of cyazofamid, 5% of metrafenone, 5% of ethylene glycol (antifreeze), 10% of N, N-dimethylacetamide (solvent), 9% of cyclohexanone (solvent), 6% of castor oil polyoxyethylene ether (emulsifier), 6% of phenethyl phenol polyoxyethylene method (emulsifier) and deionized water to make up to 100%.

Suspending agent examples

Uniformly mixing the pesticide active ingredients, the dispersing agent, the wetting agent, the thickening agent, the defoaming agent, the antifreezing agent, water and the like according to the proportion of the formula, obtaining a semi-product after wet sanding by a sand mill, adding water after analysis, uniformly mixing and filtering to obtain the suspending agent.

Example 3 50% cyazofamid-metrafenone suspension

Cyazofamid 20%, metrafenone 30%, ethylene glycol (anti-freezing agent) 5%, nekal (wetting agent) 1.5%, sodium lignosulfonate (dispersing agent) 2%, polycarboxylate (dispersing agent) 3%, xanthan gum (thickening agent) 0.08%, sodium benzoate (preservative) 0.2%, organic silicon defoamer (defoaming agent) 0.1%, and deionized water to make up to 100%.

Example 4 31% cyazofamid metrafenone suspension

Cyazofamid 1%, metrafenone 30%, ethylene glycol (anti-freezing agent) 5%, nekal (wetting agent) 1.5%, sodium lignosulfonate (dispersing agent) 5%, alkylphenol polyoxyethylene ether 2% (dispersing agent), xanthan gum (thickening agent) 0.15%, sodium benzoate (preservative) 0.2%, organic silicon defoaming agent (defoaming agent) 0.1%, and deionized water to make up to 100%.

Wettable powder examples

The pesticide active ingredients, the auxiliary agents and the fillers are uniformly mixed according to the proportion of the formula, and are mixed after being pulverized by airflow to obtain the wettable powder.

Example 5 wettable powder of 70% cyazofamid-metrafenone

Cyazofamid 10%, metrafenone 60%, sodium dodecyl sulfate (wetting agent) 2%, sodium alkyl naphthalene sulfonate (dispersing agent) 2%, polycarboxylate (dispersing agent) 2%, sodium lignin sulfonate (dispersing agent) 3%, white carbon black (filler) 2% and kaolin (filler) to 100%.

Example 6 wettable powder of 50% cyazofamid-metrafenone

Cyazofamid 30%, metrafenone 20%, sodium dodecyl sulfate (wetting agent) 2%, sodium alkyl naphthalene sulfonate (dispersant) 5%, sodium lignin sulfonate (dispersant) 6%, white carbon black (filler) 5%, and kaolin (filler) to 100%.

Examples of Water dispersible granules

The pesticide active ingredient, the auxiliary agent and the filler are uniformly mixed according to the proportion of the formula, wettable powder is obtained after airflow crushing, a certain amount of water is added, and the water dispersible granules are prepared after kneading, granulating, drying and screening.

Example 7 Water dispersible granule of cyazofamid-metrafenone 80%

Cyazofamid 20%, metrafenone 60%, sodium dodecyl sulfate (wetting agent) 2%, sodium lignosulfonate (dispersant) 3%, sodium alkylnaphthalenesulfonate (dispersant) 5%, polycarboxylate (dispersant) 5% and ammonium sulfate (filler) to 100%.

Example 8 50% cyazofamid-metrafenone Water dispersible granule

Cyazofamid 25%, metrafenone 25%, sodium dodecyl sulfate (wetting agent) 2%, sodium lignosulfonate (dispersant) 5%, sodium alkylnaphthalene sulfonate (dispersant) 5%, ammonium sulfate (disintegrant) 10%, and kaolin (filler) to 100%.

Example 9

50% of bactericidal composition, 2% of sodium dodecyl sulfate (wetting agent), 5% of sodium lignin sulfonate (dispersing agent), 5% of sodium alkyl naphthalene sulfonate (dispersing agent), 10% of ammonium sulfate (disintegrating agent) and 100% of kaolin (filler);

the bactericidal composition in the embodiment 9 comprises the following substances in percentage by mass: cyazofamid 50%, metrafenone 45%, azoxystrobin 2% and bupirimate 3%.

Example 10

the bactericidal composition in the embodiment 10 comprises the following substances in percentage by mass: cyazofamid 30%, metrafenone 65%, azoxystrobin 2% and bupirimate 3%

To further illustrate the advantages of the technical formulation scheme of the present invention, the present invention is illustrated by the following specific field test examples.

Field application example 1: field efficacy test of cyazofamid and metrafenone compound on cucumber powdery mildew

Test subjects: powdery mildew of cucumber

The test agents and the amounts are shown in table 2, and are repeated in 4 treatment cells, each cell having an area of 20 square meters. The first application is carried out at the early stage of disease occurrence, the uniform spraying is carried out until the leaves drip, and the blank control is carried out by using clear water treatment. The second dose was administered 7 days later. The onset of powdery mildew of cucumber was investigated before the first dose and 7 days after the second dose. Five-point sampling is adopted in each district, two plants are investigated in each point, all leaves are investigated in each plant, and grading record is carried out according to the percentage of lesion area to the whole leaf area. Calculating disease index and preventing and treating effect of each treatment. Grading standard:

level 0: no disease spots;

level 1: the lesion area accounts for less than 5% of the whole leaf area;

and 3, level: the lesion area accounts for 6 to 15 percent of the whole leaf area;

and 5, stage: the lesion area accounts for 16-25% of the whole leaf area;

and 7, stage: the lesion area accounts for 26-50% of the whole leaf area;

and 9, stage: the lesion area accounts for more than 50% of the whole leaf area;

the drug effect calculation method comprises the following steps:

the results of the field tests are shown in Table 2

TABLE 2 field test results of cyazofamid and metrafenone combinations on cucumber powdery mildew

As can be seen from the test results in Table 2, the control effects of the single agents of cyazofamid and metrafenone on cucumber powdery mildew are 75.82% and 77.40%, respectively, and the sterilization composition provided by the embodiment of the invention has the advantages that the control effects are obviously improved, the minimum control effect is 84.90%, and the maximum control effect is 91.55%. The results of field experiments fully show that cyazofamid and metrafenone are compounded to have remarkable synergistic effect on cucumber powdery mildew, and the control effect is greatly improved under the condition that the dosage of the effective components per mu is reduced compared with that of a single agent. Therefore, the composition has the beneficial effects of reducing cost, delaying resistance, reducing the application times and reducing pesticide residues.

Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.

Claims

1. The bactericidal composition containing cyazofamid and metrafenone is characterized by comprising the following components in parts by weight: the bactericidal composition comprises cyazofamid and metrafenone, wherein the mass ratio of cyazofamid to metrafenone is 50:1-1: 50.

2. The germicidal composition of claim 1, wherein: the mass ratio of cyazofamid to metrafenone is 20:1-1: 20.

3. The germicidal composition of claim 1, wherein: the total mass of the cyazofamid and the metrafenone accounts for 1-90% of the mass of the bactericidal composition.

4. The germicidal composition of claim 1, wherein: the bactericidal composition further comprises the following substances in percentage by mass: 1-2% of azoxystrobin.

5. The germicidal composition of claim 1, wherein: the bactericidal composition comprises the following substances in percentage by mass: cyazofamid 50%, metrafenone 40%, azoxystrobin 2%, polyoxyethylene 3% and bupirimate 5%.

6. The germicidal composition of claim 1, wherein: the bactericidal composition comprises the following substances in percentage by mass: cyazofamid 30%, metrafenone 65%, azoxystrobin 2% and bupirimate 3%.

7. The germicidal composition of claim 1, wherein: the dosage form of the bactericidal composition is one of microemulsion, aqueous emulsion, suspending agent, wettable powder and water dispersible granules.

8. The germicidal composition of claim 1, wherein: the bactericidal composition is used for powdery mildew on crops such as vegetables, melons and fruits.

9. A bactericide comprising the fungicidal composition of any of claims 1 to 7, which comprises, by mass, 25% cyazofamid, 22.5% metrafenone, 1% azoxystrobin and 1.5% bupirimate, 2% sodium dodecyl sulfate (wetting agent), 5% sodium lignosulfonate (dispersant), 5% sodium alkylnaphthalenesulfonate (dispersant), 10% ammonium sulfate (disintegrant), and kaolin (filler) to make up to 100%.