CN112574907A - New strain of northern industrial and commercial pseudoxanthomonas and application thereof - Google Patents
New strain of northern industrial and commercial pseudoxanthomonas and application thereof Download PDFInfo
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- CN112574907A CN112574907A CN202011423248.0A CN202011423248A CN112574907A CN 112574907 A CN112574907 A CN 112574907A CN 202011423248 A CN202011423248 A CN 202011423248A CN 112574907 A CN112574907 A CN 112574907A
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- pseudoxanthomonas
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/02—Preparation of other alcoholic beverages by fermentation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a new strain of northern industrial and commercial Pseudoxanthomonas and application thereof, wherein the strain is northern industrial and commercial Pseudoxanthomonas (Pseudomonas beignogongshangensis) and the preservation number of the strain in the common microorganism center of China Committee for culture Collection of microorganisms is CGMCC NO. 19208. Experiments prove that the new strain, namely, the northern industrial and commercial Pseudoxanthomonas (Pseudoxanthomonas beignogngshengensis), is separated from the white spirit cellar mud, can generate aromatic flavor substances, and can be used for fermenting and brewing wine.
Description
Technical Field
The invention relates to the technical field of brewing microorganisms, and particularly relates to a new strain of northern industrial and commercial pseudoxanthomonas and application thereof.
Background
White spirit is one of three distilled spirits in the world, is produced by adopting a unique fermentation technology and has a history of thousands of years. The whole inner wall of the pit is covered with pre-cultured pit mud. The fermented raw materials are cooked, mixed, crushed and distilled. Adding a saccharification leaven into the steamed raw materials, then putting the steamed raw materials into a cellar for fermentation, taking the fermented materials out of the cellar, and distilling the fermented materials to prepare the Chinese liquor. Microorganisms in pit mud produce various flavor components such as butyric acid, caproic acid and ethyl caproate. Ethyl caproate is considered as a key ingredient affecting the flavor and quality of Luzhou-flavor liquor. In the brewing process, the pit mud microorganisms have important aroma-producing and flavor-enhancing effects, and the quality of the Luzhou-flavor liquor is improved along with the increase of the age of the wine pit. The high quality of the Luzhou-flavor liquor is attributed to the maturation process of pit mud, which results in the balance of microbial community structures and the diversity of pit mud, thereby generating unique flavor.
The strong aromatic Chinese spirits have the flavor characteristics of strong cellar aroma, sweet and mellow taste, harmonious aroma and long aftertaste, are deeply favored by consumers, and the annual sale amount accounts for about 70 percent of the total amount of Chinese spirits. Microorganisms in pit mud can generate various flavor components such as butyric acid, caproic acid and ethyl caproate, and the ethyl caproate is considered as a key component influencing the flavor and the quality of the Luzhou-flavor liquor. The pit mud microbial flora is a main factor influencing the quality of pit mud and white spirit, and is an important microbial source in the brewing process of the white spirit. The microorganisms in the pit mud are various and have very rich species diversity, but most of the microorganisms are still in a difficult culture state. Although the culturability of microorganisms in different environments is different, the culturability is very low, for example, the culturability in seawater is less than 0.1 percent, and the culturability in soil is about 0.3 percent. Only a small proportion of bacteria in a natural habitat can be cultured using conventional bacterial culture methods. The microorganisms cultured by the traditional method are all known microorganisms, and no new microorganism species is found.
Disclosure of Invention
Therefore, the invention provides a new strain of northern industrial and commercial pseudoxanthomonas and application thereof.
In order to achieve the above purpose, the invention provides the following technical scheme:
the preservation number of the northern industrial and commercial Pseudoxanthomonas (Pseudomonas beignong shangensis) in the common microorganism center of China Committee for culture Collection of microorganisms is CGMCC NO. 19208.
The application of the Pseudoxanthomonas sobria or the fermentation product or the culture product thereof for producing flavor substances in the brewing process also belongs to the protection scope of the invention.
The invention also provides a microbial agent containing the pseudoxanthomonas sobria.
In one embodiment of the invention, the 16S rDNA sequence of the Pseudoxanthomonas gondii is shown in SEQ ID No. 1.
The invention also provides a screening culture method of the northern industrial and commercial pseudoxanthomonas, which comprises the steps of diluting the pit mud, coating the diluted pit mud on an R2A culture medium, and screening and culturing to obtain the northern industrial and commercial pseudoxanthomonas.
In one embodiment of the invention, in the screening culture method of pseudoxanthomonas sobria, the R2A culture medium comprises the following raw materials by mass: 0.5g of yeast extract powder, 0.3g of sodium pyruvate, 0.5g of peptone, 0.024 of anhydrous magnesium sulfate, 0.5g of glucose, 0.3g of dipotassium hydrogen phosphate, 0.5g of soluble starch, 0.5g of casein hydrolysate and 15g of agar.
In one embodiment of the invention, the time of the screening culture is 20-28 hours.
In one embodiment of the invention, the screening culture temperature is 35-40 ℃.
The invention also provides the application of the strain or the microbial inoculum in the following (A1) or (A2): (A1) generating a flavor substance; (A2) a product with a flavour material is prepared.
The invention also provides the application of the strain or the microbial inoculum in the following (B1) or (B2): (B1) brewing wine; (B2) and (5) preparing a wine brewing product.
The invention has the following advantages:
experiments prove that the new strain provided by the invention is Pseudoxanthomonas beigongensis (Pseudoxanthomonas beignogongensis), is separated from pit mud, can generate aromatic flavor substances, and can be used for fermenting and brewing wine.
The invention separates the northern industrial and commercial Pseudoxanthomonas (Pseudoxanthomonas beignoggensis) strain, firstly adopts an R2A culture medium, the common bacteria are slow in propagation, and the difficultly-cultured new Pseudoxanthomonas bacteria grow fast on the same culture dish and are slightly influenced by the conventional microorganisms; meanwhile, after the culture time is prolonged for 36 hours, a single colony is picked, and new microorganisms difficult to culture grow fully for a long time until the colony is visible.
Bacterial preservation description: the northern industrial and commercial Pseudoxanthomonas (Pseudoxanthomonas beignoggensis) is preserved in the common microorganism center of China general microbiological culture Collection management Committee in 2019, 12 and 16 days, and the preservation address is as follows: the preservation number of the microbial research institute of Chinese academy of sciences, No. 3 Xilu No.1 of Beijing, Chaoyang, and the institute of microbiology, is CGMCC NO. 19208.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.
FIG. 1 is a 16S rDNA sequence and a tree diagram of Pseudoxanthomonas sobria provided by the present invention;
FIG. 2 is a diagram showing the detection result of respiratory quinone ubiquinone Q-8 of Pseudoxanthomonas sobria provided by the present invention;
FIG. 3 is a diagram showing the detection result of polar ester of Pseudoxanthomonas sobria.
Detailed Description
The present invention is described in terms of particular embodiments, other advantages and features of the invention will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the invention and that it is not intended to limit the invention to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1 isolation and characterization of Pseudoxanthomonas beignongshanensis
Isolation of novel species of Pseudomonas beigongensis
Step one, preparing an R2A culture medium: 0.5g of yeast extract powder, 0.3g of sodium pyruvate, 0.5g of peptone, 0.024g of anhydrous magnesium sulfate, 0.5g of glucose, 0.3g of dipotassium hydrogen phosphate, 0.5g of soluble starch, 0.5g of casein hydrolysate and 15g of agar;
step two, taking 10g of pit mud, wherein the pit mud is from Sichuan strong aromatic wineries and is diluted to 10 percent by using sterile distilled water-5Coating on a prepared R2A culture medium culture dish for aerobic culture at 28 ℃;
step three, carrying out aerobic culture at 28 ℃ for 36 hours, then selecting a single colony, and carrying out streak purification on three regions for 3 times to obtain pure culture bacteria;
and step four, extracting pure culture bacterial DNA, amplifying a 16SrDNA sequence by utilizing a PCR technology, and preliminarily determining a new strain of the northern industrial and commercial pseudoxanthomonas.
Identification of Erythrochlogonophytes in North and second industries
The new bacteria separated and screened from pit mud are gram-negative bacteria, pseudoxanthomonas, named as: pseudoxanthomonas beigongensis (Pseudoxanthomonas beigongensis)
1. The northern industry commercial Pseudoxanthomonas (Pseudoxanthomonas beignonggensis) is used for morphological, physiological and biochemical, cytochemical and gene level research of strains. Physiological and biochemical characteristics of Pseudoxanthomonas beigondii REN9 (Pseudomonas beigongensis)
The colony morphology of northern industrial and commercial Pseudoxanthomonas (Pseudoxanthomonas beignongshangensis) is round, the surface is smooth, and the Pseudoxanthomonas beignongshangensis is faint yellow and has no spores; gram-negative bacteria, the optimum temperature is 37 ℃, the optimum pH value is 7, the optimum sodium chloride concentration is 2%, the starch can be hydrolyzed, the catalase is positive, the esterase (C4), the lipoid esterase (C8), the colipase (C14), the leucine arylaminase, the valine arylaminase, the cystine arylaminase, the trypsin, the chymotrypsin, the acid phosphatase, the naphthol-AS-BI-phosphohydrolase, the beta-galactosidase and the N-acetyl-glucosaminidase are positive, and the glucosidase-esculin is hydrolyzed by fermentation (glucose).
2. Northern industrial and commercial pseudoxanthomonas cytochemical characteristic detection
The cytochemical components of fatty acids, quinone type, polar lipids, etc., of Pseudoxanthomonas sobria (Pseudomonas beignogongensis) were detected by GC gas chromatography, HPLC liquid chromatography and TLC thin-layer chromatography (Sasser M.identification of bacterial by gas chromatography of cellular fat acids, MIDI Technical Note 101.Newark, DE: MIDIinc; 1990.Minnikin DE, O' Donnell AG, Goodlfow M, Alderson G, Atharye M et al. integrated procedure for the extraction of bacterial isopropyl reagents and alcohols lipids. J. Microbiol. Methods 1984; 2: 233).
Fatty acid component of Pseudomonas beigonensis (Pseudomonas beignonggensis) cell: saturated fatty acids: c16:0, Antesio-C15:0, Iso-C13:0, Iso-C14:0, Iso-C15:0, Iso-C16: 0; unsaturated fatty acid: c16: 0N alcohol, C16:1 omega 7C alcohol, C16:1 omega 7C alcohol, C16:1 omega 11C cell fatty acid composition are shown in Table 1
TABLE 1
As shown in figure 2, in the detection of quinone component in the cell respiration of Pseudomonas beigongensis (Pseudomonas beignogongensis), the main advantage of the strain is quinone ubiquinone Q-8, and quinone compounds are a class of oxidation active substances which are widely existed in natural products, anti-tumor drugs, in-vivo biochemical metabolites, environmental pollutants or polycyclic aromatic hydrocarbon metabolism. Each bacterium has a main quinone component, and the difference in the kinds and amounts of quinones in a microbial population reflects the diversity of the population composition, and the quinone spectrum has been widely used in environmental microorganisms as an index of the diversity of the bacterial population composition.
The detection of polar ester of northern industrial and commercial Pseudoxanthomonas (Pseudoxanthomonas beignongshangensis) comprises the following specific steps: polar lipid extraction: 100mg of lyophilized thallus of northern industrial and commercial Pseudoxanthomonas campestris was suspended in 9.5mL of chloroform: methanol: 0.3% NaCl (2.5:5: 2). The thallus solution is placed in a water bath at 80 ℃ for 15 min. After cooling, the filter paper was filtered into a 50mL centrifuge tube, 2.5mL chloroform and 2.5mL 0.3% NaCl were added, and centrifuged at 4000rpm for 5 min. The lower chloroform phase was carefully separated into a clean rotary evaporator-dedicated flask and the chloroform removed by rotary evaporation under reduced pressure on the rotary evaporator, the temperature of the water bath not exceeding 40 ℃. 250 mu.L of chlorine anti-methanol (2:1, v/v) is added and transferred to a brown screw sample bottle, and the bottle is placed in a refrigerator at 4 ℃ for storage and testing.
TLC analysis of polar lipids: a10 cm × 10cm Silica gel plate (Merck 25TLC aluminum sheets 20cm × 20cm Silica gel 60F254) was activated in an oven at 110 deg.C for 1 hour, and then cooled. Pipette 2 μ L of total lipid sample onto TLC plate, and spot 3 times.
And (3) placing the TLC thin plate into a first chromatographic cylinder for layer development, wherein the first spreading agent is chloroform, methanol and water (65:25:4, v/v), taking out the thin plate after the solvent is spread to the top, drying the thin plate by blowing, placing the thin plate into a second chromatographic cylinder, and the second spreading agent is chloroform: methanol: acetic acid: water (80: 12: 15: 4, V/V), ascending in a direction perpendicular to the first direction, spreading the solvent to the top, taking out the thin plate for drying, spraying phosphomolybdic acid color developing agent to the TLC plate until the TLC plate is completely wet, heating at 100 ℃ for 5-8min, displaying clear spots, immediately scanning the TLC plate on a scanning instrument, and recording the result. As shown in fig. 3, the north industrial pseudoxanthomonas species contains 4 polar esters, DPG: diphosphatidylglycerol, diphosphatidylglycerol; PG: phosphatidylglycerol, phosphonoglycolic, glycophospholipidid; PE phosphatidylethanolamine, phosphatidyl ethanolamines. And the protein, the sugar and the like form cell membranes, and play an important role in substance transportation, metabolism and normal osmotic pressure maintenance. The phospholipidic components of different genera of bacteria are different, and are one of the important characteristics for identifying the genus, and are indispensable classification indicators in chemical classification projects.
3. Pseudoxanthomonas gondii 16S rDNA sequence and evolutionary tree
The bacterial strain of the northern industrial and commercial pseudoxanthomonas is subjected to whole genome sequencing by Shanghai Meiji biological medicine science and technology limited company to obtain a whole genome sequence. The phylogenetic tree is shown in figure 1.
The measured REN9T 16S rRNA sequence was compared with the model strain in NCBI (https:// www.ncbi.nlm.nih.gov /), 30 model strain 16S rRNA sequences were selected according to the similarity to construct a phylogenetic tree, as shown in FIG. 1, REN9T was close to the relative distance of the P.pseudoxanthomonas and was an independent branch in the phylogenetic tree, and was determined to be P15, while the similar strain was determined to be Pseudomonas indica P15 according to the similarity (98.98%). Then, REN9T strain is sent to Shanghai Meiji biological medicine science and technology limited to perform whole genome sequencing, so as to obtain a whole genome sequence. At the same time, the whole genome of a similar strain was downloaded from NCBI, and the whole genome of REN9T was aligned with the whole genome of a similar strain Pseudomonas indica P15 at DSMZ (http:// ggdc. dsmzz. de/home. php), and genomic DNA-DNA homology hybridization (DDH): DNA Homology (DDH) is 19.60% [ 17.4-22% ], and DDH is less than 70%, and thus it was preliminarily determined as a new species.
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Sequence listing
<110> Beijing university of Industrial and commercial
<120> new strain of northern industrial and commercial pseudoxanthomonas and application thereof
<130> GG19719816A
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1438
<212> DNA
<213> Artificial Sequence
<400> 1
ccgggggtgg cctaaccatg caagtcgaac ggcagcacag gagagcttgc tctctgggtg 60
gcgagtggcg gacgggtgag gaatacatcg gaatctacct tgtcgtgggg gataacgtag 120
ggaaacttac gctaataccg catacgacct tcgggtgaaa gtgggggacc gcaaggcctc 180
acgcgattag atgagccgat gtcggattag ctagttggcg gggtaaaagc ccaccaaggc 240
gacgatccgt agctggtctg agaggatgat cagccacact ggaactgaga cacggtccag 300
actcctacgg gaggcagcag tggggaatat tggacaatgg gcgcaagcct gatccagcca 360
taccgcgtgg gtgaagaagg ccttcgggtt gtaaagccct tttgttggga aagaaatctt 420
gccggttaat acctggcgag gatgacggta cccaaagaat aagcaccggc taacttcgtg 480
ccagcagccg cggtaatacg aagggtgcaa gcgttactcg gaattactgg gcgtaaagcg 540
tgcgtaggtg gtggtttaag tctgctgtga aagccctggg ctcaacctgg gaattgcagt 600
ggatactggg tcactagagt gtggtagagg gatgcggaat ttccggtgta gcagtgaaat 660
gcgtagagat cggaaggaac atccgtggcg aaggcggcat cctgggccaa cactgacact 720
gaggcacgaa agcgtgggga gcaaacagga ttagataccc tggtagtcca cgccctaaac 780
gatgcgaact ggatgttggg tgcaacttgg cacccagtat cgaagctaac gcgttaagtt 840
cgccgcctgg ggagtacggt cgcaagactg aaactcaaag gaattgacgg gggcccgcac 900
aagcggtgga gtatgtggtt taattcgatg caacgcgaag aaccttacct ggccttgaca 960
tgtcgcgaac tttccagaga tggattggtg ccttcgggaa cgcgaacaca ggtgctgcat 1020
ggctgtcgtc agctcgtgtc gtgagatgtt gggttaagtc ccgcaacgag cgcaaccctt 1080
gtccttagtt gccagcacgt aatggtggga actctaagga gaccgccggt gacaaaccgg 1140
aggaaggtgg ggatgacgtc aagtcatcat ggcccttacg gccagggcta cacacgtact 1200
acaatgggaa ggacagaggg ctgcaaaccc gcgagggcaa gccaatccca gaaaccttct 1260
ctcagtccgg attggagtct gcaactcgac tccatgaagt cggaatcgct agtaatcgca 1320
gatcagcatt gctgcggtga atacgttccc gggccttgta cacaccgccc gtcacaccat 1380
gggagtttgt tgcaccagaa gcaggtagct taaccttcgg gagggcgcct gccccagc 1438
Claims (10)
1. The preservation number of the northern industrial and commercial Pseudoxanthomonas (Pseudoxanthomonas beignongshangensis) in the common microorganism center of China Committee for culture Collection of microorganisms is CGMCC NO. 19208.
2. Use of the Pseudoxanthomonas sobria or the fermentation product or the culture product thereof according to claim 1 for producing flavor substances in the brewing process.
3. A microbial agent comprising pseudoxanthomonas sobria as claimed in claim 1 or 2.
4. The Pseudoxanthomonas sobria as claimed in claim 1, wherein the 16S rDNA sequence is shown in SEQ ID No. 1.
5. The method for screening and culturing pseudoxanthomonas sobria as claimed in claim 1, which comprises diluting cellar mud, spreading the diluted cellar mud on R2A culture medium, and screening and culturing to obtain the pseudoxanthomonas sobria.
6. The method of claim 5, wherein the culture medium is selected from Pseudoxanthomonas sp,
the R2A culture medium comprises the following raw materials in mass: 0.5g of yeast extract powder, 0.3g of sodium pyruvate, 0.5g of peptone, 0.024 of anhydrous magnesium sulfate, 0.5g of glucose, 0.3g of dipotassium hydrogen phosphate, 0.5g of soluble starch, 0.5g of casein hydrolysate and 15g of agar.
7. The method of claim 5, wherein the culture medium is selected from Pseudoxanthomonas sp,
the time of the screening culture is 20-28 hours.
8. The method of claim 5, wherein the culture medium is selected from Pseudoxanthomonas sp,
the screening culture temperature is 35-40 ℃.
9. Use of the strain of claim 1 or the microbial agent of claim 3 in (A1) or (A2) as follows:
(A1) generating a flavor substance;
(A2) a product with a flavour material is prepared.
10. Use of the strain of claim 1 or the microbial agent of claim 3 in (B1) or (B2) as follows:
(B1) brewing wine;
(B2) and (5) preparing a wine brewing product.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011423248.0A CN112574907A (en) | 2020-12-08 | 2020-12-08 | New strain of northern industrial and commercial pseudoxanthomonas and application thereof |
| ZA2021/10015A ZA202110015B (en) | 2020-12-08 | 2021-12-06 | New pseudoxanthomonas beigongshangensis strain and use thereof |
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| CN202011423248.0A CN112574907A (en) | 2020-12-08 | 2020-12-08 | New strain of northern industrial and commercial pseudoxanthomonas and application thereof |
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| CN202011423248.0A Pending CN112574907A (en) | 2020-12-08 | 2020-12-08 | New strain of northern industrial and commercial pseudoxanthomonas and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113308406A (en) * | 2021-06-09 | 2021-08-27 | 北京工商大学 | New strain of Beijing swimming micro-fungus and application thereof |
| CN113881640A (en) * | 2021-10-13 | 2022-01-04 | 福建农林大学 | Pseudomonas homoandra phage and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250794A (en) * | 2011-06-21 | 2011-11-23 | 中国农业科学院农业环境与可持续发展研究所 | Pseudoxanthomonasjaponensis and microorganism microbial inoculum as well as applications thereof |
| CN109666605A (en) * | 2018-12-26 | 2019-04-23 | 清华大学 | A kind of cellulose bioconversion method of artificial flora |
-
2020
- 2020-12-08 CN CN202011423248.0A patent/CN112574907A/en active Pending
-
2021
- 2021-12-06 ZA ZA2021/10015A patent/ZA202110015B/en unknown
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250794A (en) * | 2011-06-21 | 2011-11-23 | 中国农业科学院农业环境与可持续发展研究所 | Pseudoxanthomonasjaponensis and microorganism microbial inoculum as well as applications thereof |
| CN109666605A (en) * | 2018-12-26 | 2019-04-23 | 清华大学 | A kind of cellulose bioconversion method of artificial flora |
Non-Patent Citations (2)
| Title |
|---|
| JIALIANG XU等: "Culturing Bacteria From Fermentation Pit Muds of Baijiu With Culturomics and Amplicon-Based Metagenomic Approaches", 《FRONTIERS IN MICROBIOLOGY》 * |
| REN,Q.: "GenBank：MT269284.1", 《GENBANK》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113308406A (en) * | 2021-06-09 | 2021-08-27 | 北京工商大学 | New strain of Beijing swimming micro-fungus and application thereof |
| CN113308406B (en) * | 2021-06-09 | 2023-01-31 | 北京工商大学 | New strain of Beijing swimming micro-fungus and application thereof |
| CN113881640A (en) * | 2021-10-13 | 2022-01-04 | 福建农林大学 | Pseudomonas homoandra phage and application thereof |
| CN113881640B (en) * | 2021-10-13 | 2022-09-09 | 福建农林大学 | Pseudomonas homoandra phage and application thereof |
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| Publication number | Publication date |
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| ZA202110015B (en) | 2022-03-30 |
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