CN112568326A - Anti-stress composite premixed feed for laying fowls and preparation method and application thereof - Google Patents
Anti-stress composite premixed feed for laying fowls and preparation method and application thereof Download PDFInfo
- Publication number
- CN112568326A CN112568326A CN202011452817.4A CN202011452817A CN112568326A CN 112568326 A CN112568326 A CN 112568326A CN 202011452817 A CN202011452817 A CN 202011452817A CN 112568326 A CN112568326 A CN 112568326A
- Authority
- CN
- China
- Prior art keywords
- parts
- laying
- stress
- feed
- protease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Inorganic Chemistry (AREA)
- Birds (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention relates to an anti-stress compound premix feed for laying hens and a preparation method and application thereof. The premixed feed consists of the following raw materials in parts by weight: 160-240 parts of citric acid, 80-160 parts of fumaric acid, 8-15 parts of plant essential oil compound, 2-5 parts of 25-hydroxycholecalciferol, 0.15-0.75 part of ammonium sulfate nitrate, 6-15 parts of nicotinamide, 0.2-0.5 part of riboflavin, 0.2-0.5 part of D-calcium pantothenate, 1-2 parts of pyridoxine hydrochloride, 20-36 parts of DL-alpha-tocopheryl acetate, 0.4-0.7 part of menadione sodium bisulfite, 1-4 parts of biotin, and vitamin B120.3-0.9 part of phytase, 10-30 parts of protease, 1-5 parts of protease, 2-10 parts of DL-methionine, 20-60 parts of yeast selenium, 50-150 parts of calcium hydrophosphate and 200-600 parts of carrier. The premixed feed provided by the invention can obviously improve stress intestinal injury of laying poultry, improve digestion and absorption capacity, finally relieve stress of the laying poultry, reduce dirty egg rate, improve laying rate in a stress state, improve egg quality and breeding performance of breeding poultry, reduce dirty egg rate, improve fertility rate and hatching rate, and increase economic benefits of farmers.
Description
Technical Field
The invention belongs to the technical field of poultry nutrition and regulation and control, and particularly relates to an anti-stress compound premix feed for laying hens as well as a preparation method and application thereof.
Technical Field
Many stresses are encountered during modern intensive poultry farming, such as cold, heat stress, etc., and the causes of stress are many, such as transportation, temperature changes, refueling, insect repelling, exposure to antibiotics and other chemicals, bacteria, viruses and parasites, vaccination, beak breaking, overcrowding, farm conditions being unsatisfactory, etc. These factors leave the chicken under stress at any time throughout life, adversely affecting growth, production and feed conversion efficiency in vivo. Particularly for laying fowls, the laying period is physiological stress, a large amount of ROS (oxygen free radicals) are generated in the ovulation process, continuous large-amount ovulation of high-yield laying fowls causes oxidative damage accumulation of ovaries, livers and the like, the expression of gonadotropin receptors of follicular granulosa cells is inhibited, the sensitivity of the follicular granulosa cells to gonadotropin is reduced, the number of follicles is reduced, the atresia rate is increased, and the factor is one of important factors for reducing the laying rate in the laying period.
In the face of various stresses, the nervous system, endocrine system, immune system and gastrointestinal tract of the body all produce adaptive responses to maintain homeostasis. Stress generally regulates the secretion of catecholamines and glucocorticoids through the secretory cascade of the locus coenospora-adrenal medulla axis and the hypothalamus-pituitary-adrenal cortex axis to mediate necessary adaptability regulation, and glucocorticoids regulate energy balance to cope with energy challenges caused by stressors, which is of great significance for improving stress adaptability, but can cause insufficient energy for production and reproduction, also can cause harm to the health of organisms, ischemic injury of gastrointestinal tracts, oxidative stress injury of intestinal wall cells and liver cells, body inflammation and immunity reduction, thereby increasing animal susceptible diseases, reducing the production efficiency of animals and failing to fully exert genetic potential.
Both acute and sustained stress have been found to induce oxidative stress in the gastrointestinal tract by producing free radicals (Manashi, 2000). Oxidative stress has many deleterious effects on cells and tissues, including oxidation of lipids, proteins and DNA, depletion of cellular energy, release of intracellular calcium ions, and some important disorders of cellular metabolism. Ovulation is a reaction that resembles the development of inflammation, with the concomitant production of large amounts of ROS (Miyamoto et al, 2010), which at physiological levels help to maintain the redox state and signaling of cells, but excess levels cause oxidative stress in cells and tissues. Oxidative stress caused by the gradual accumulation of ROS in ovarian tissues is a major factor leading to a decrease in ovarian function (Lim et al, 2015), and ovarian dysfunction is responsible for a decrease in egg production after peak laying, a decrease in the number of consecutive eggs, and a prolongation of the mean egg laying interval.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides an anti-stress compound premix feed for laying hens and a preparation method and application thereof. The premix feed has natural components, reasonable formula and no other hormone, can effectively relieve stress of laying poultry, repair stress organism injury, and improve egg laying performance, egg quality and reproductive performance of breeding poultry of the laying poultry.
The technical scheme of the invention is as follows:
an anti-stress compound premix feed for laying hens is composed of the following raw materials in parts by weight: 160-240 parts of citric acid, 80-160 parts of fumaric acid, 8-15 parts of plant essential oil compound, 2-5 parts of 25-hydroxycholecalciferol, 0.15-0.75 part of ammonium sulfate nitrate, 6-15 parts of nicotinamide, 0.2-0.5 part of riboflavin, 0.2-0.5 part of D-calcium pantothenate, 1-2 parts of pyridoxine hydrochloride, 20-36 parts of DL-alpha-tocopheryl acetate, 0.4-0.7 part of menadione sodium bisulfite, 1-4 parts of biotin, and vitamin B120.3-0.9 part of phytase, 10-30 parts of protease, 1-5 parts of protease, 2-10 parts of DL-methionine, 20-60 parts of yeast selenium, 50-150 parts of calcium hydrophosphate and 200-600 parts of carrier.
According to the invention, the anti-stress compound premix feed for the eggs is preferably prepared from the following raw materials in parts by weight: 170-210 parts of citric acid, 90-150 parts of fumaric acid, 9-13 parts of plant essential oil compound, 2-4 parts of 25-hydroxycholecalciferol, 0.2-0.6 part of ammonium sulfate nitrate, 8-12 parts of nicotinamide, 0.25-0.45 part of riboflavin, 0.25-0.45 part of D-calcium pantothenate, 1.2-2 parts of pyridoxine hydrochloride, 20-32 parts of DL-alpha-tocopheryl acetate, 0.45-0.65 part of menadione sodium bisulfite, 1.2-3.6 parts of biotin, and vitamin B120.35-0.85 part of phytase, 15-30 parts of protease, 2-5 parts of protease, 3-9 parts of DL-methionine, 20-50 parts of yeast selenium, 60-140 parts of calcium hydrophosphate and 250-550 parts of carrier.
Preferably, according to the present invention, the plant essential oil complex is prepared according to the following method: uniformly mixing carvacrol and eugenol according to the mass ratio of 2:1, and adding silicon dioxide for adsorption to obtain the plant essential oil compound, wherein the total mass of carvacrol and eugenol is more than 37.5%.
Further preferably, the carvacrol is synthesized by organic chemical engineering, and the content of active ingredients is more than or equal to 90%; the eugenol is volatile oil obtained by distilling dry bud flos Caryophylli of Myrtaceae, and is yellowish or colorless clear oily substance with special fragrance of flos Caryophylli, and effective component is greater than or equal to 90%.
According to the invention, the DL-alpha-tocopherol acetate is feed-grade water-soluble DL-alpha-tocopherol acetate powder, and the content of the DL-alpha-tocopherol acetate is more than or equal to 50%.
Preferably, according to the invention, the product compositional analysis assurance value of the 25-hydroxycholecalciferol is greater than or equal to 0.125%; the biotin is feed-grade biotin, and the content of the biotin is more than or equal to 2%; the vitamin B12Is feed grade vitamin B12The content is more than or equal to 1 percent.
According to the invention, the phytase is preferably derived from Aspergillus niger or Pichia pastoris, and the activity is 4000-6000 IU/kg.
According to the invention, the protease is preferably derived from Aspergillus niger or Bacillus subtilis, and the activity is 40000-60000 IU/kg.
According to the invention, the carrier is preferably one or a mixture of more than two of pine needle powder, soybean phospholipid oil powder, medical stone, zeolite powder, stone powder, anhydrous sodium sulphate and silicon dioxide.
The preparation method of the anti-stress compound premix feed for the laying fowl comprises the following steps:
1) mixing ammonium sulfate nitrate, riboflavin, nicotinamide, D-calcium pantothenate, pyridoxine hydrochloride, menadione sodium bisulfite, biotin, and vitamin B12Mixing 25-hydroxycholecalciferol, protease, DL-methionine and part of carrier, and sieving to form premix A;
2) adding fumaric acid, citric acid, a plant essential oil compound, DL-alpha-tocopherol acetate, phytase, premix A, calcium hydrophosphate, selenium yeast and the rest of carriers into a mixer, mixing for 5-10 minutes, and sieving to obtain the anti-stress composite premixed feed for the eggs and the poultry.
According to the invention, preferably, in the step (1) and the step (2), a 2-3 mm analysis sieve is adopted for sieving, so that the variation coefficient of the mixing uniformity is not more than 5%.
The invention also provides application of the anti-stress compound premix feed for the laying fowls in the process of breeding the laying fowls, which is used for resisting stress of the laying fowls, and the specific application method is as follows: the feed additive is added into the feed according to the proportion of 0.5-1.0% of the total mass of the feed.
According to the invention, through the research on the regulation mechanism of the stress state of an organism, the traditional anti-stress scheme of vitamin, amino acid, electrolyte and energy is abandoned, and the method comprehensively starts from the aspects of oxidative stress, stress intestinal injury, stress immunosuppression, stress nutrient consumption and the like in a multiple way. The novel anti-stress scheme is provided, namely, the novel anti-stress scheme comprises an acidifier, a plant essential oil compound, vitamins, amino acid, selenium and an enzyme preparation, so that the oxidative stress of an organism is reduced and relieved, and the stress organism injury is repaired; meanwhile, the intestinal health is regulated and controlled, and the digestion, absorption and deposition of nutrient substances are improved; supplementing nutrition, improving immune response level, and improving egg laying performance, egg quality and reproductive performance of breeding fowl.
The invention has the technical characteristics and beneficial effects that:
(1) according to the invention, through the combination of DL-alpha-tocopheryl acetate, selenium yeast, citric acid and plant essential oil compound, the three-level antioxidant system of the effective tissues of the laying fowl can be strengthened, the formation of free radicals can be prevented, the formed free radicals can be eliminated, the formation and proliferation of chains can be limited, and the damage of cell molecules of a stress organism can be repaired and eliminated. And the vitamins are used as coenzymes for metabolism in vivo, promote metabolism of glucose, protein and lipid substances, and improve the immune response capability.
(2) According to the invention, through the combination of citric acid, fumaric acid, a plant essential oil compound, phytase and protease, the intestinal tract tight connection and the intestinal lumen microecological balance can be regulated and controlled, the intestinal tract mucosa slgA secretion is enhanced, the intestinal tract barrier function is regulated and controlled, and the digestion and absorption of intestinal tract nutrient substances are promoted.
(3) The premix feed is added into basic ration of laying fowls, stress intestinal injury can be obviously improved, barrier function of intestinal tracts can be repaired, digestion and absorption capacity can be improved, liver fat metabolism level can be improved, health and function of intestinal axis and hepatic axis can be maintained, stress of the laying fowls can be relieved, dirty egg rate can be reduced, laying rate in stress state can be improved, laying rate reduction in laying period can be delayed, egg quality and breeding performance of the breeding fowls can be improved, dirty egg rate can be reduced, fertility rate and hatching rate of the breeding fowls can be improved, genetic potential of organisms can be effectively exerted, and economic benefit of farmers can be increased.
Detailed Description
The following examples and test examples are intended to illustrate the present invention, but are not intended to limit the scope of the present invention.
The materials involved in the invention are all common commercial products.
Example 1
An anti-stress compound premix feed for laying hens is composed of the following raw materials by weight:
| raw materials | Specification of raw materials | Ratio/kg |
| Citric acid | 99.5-100.5％ | 180 |
| Fumaric acid | ≥99.0％ | 100 |
| Plant essential oil compound | The content of eugenol is more than or equal to 12.5 percent, and the content of carvacrol is more than or equal to 25 percent | 10 |
| 25-hydroxyl gallbladderCalciferols | 0.125％ | 3 |
| Ammonium sulfate nitrate | ≥98％ | 0.4 |
| Nicotinamide | ≥99％ | 10 |
| Riboflavin | 98.0％-102.0％ | 0.3 |
| D-calcium pantothenate | 98.0-101.0％ | 0.4 |
| Pyridoxine hydrochloride | 98.0％-101.0％ | 1.8 |
| DL-alpha-tocopheryl acetate | 50％ | 20 |
| Menadione sodium bisulfite | Calculated by menadione 50 percent | 0.6 |
| Biotin | 2％ | 2 |
| Vitamin B12 | 1％ | 0.5 |
| Phytase | 5000 units of | 30 |
| Protease enzyme | 50000 units | 5 |
| DL-methionine | ≥98.5％ | 8 |
| Selenium yeast | 0.10％ | 20 |
| Silicon dioxide | Feed grade | 5 |
| Calcium hydrogen phosphate | Feed grade | 100 |
| Soybean lecithin oil powder | Feed grade | 150 |
| Medical stone | Feed grade | 353 |
| Total up to | 1000 |
The plant essential oil compound is prepared by the following method: mixing carvacrol 2.5kg and eugenol 1.25kg uniformly by using a homogenizing device, then adding silicon dioxide 6.25kg to adsorb essential oil, mixing uniformly, and sieving by using a 2mm analysis sieve to obtain plant essential oil compound 10 kg.
The preparation method of the anti-stress compound premix feed for the laying fowl comprises the following steps:
(1) 0.4kg of ammonium sulfate nitrate, 0.3kg of riboflavin, 10kg of nicotinamide, 0.4kg of D-calcium pantothenate, 1.8kg of pyridoxine hydrochloride, 0.6kg of menadione sodium bisulfite, 2kg of biotin and vitamin B12Weighing 0.5kg, 3kg of 25-hydroxycholecalciferol and 5kg of protease according to the weight, adding 5kg of silicon dioxide and 20kg of medical stone, uniformly mixing, and sieving by using a 2mm analysis sieve to obtain premix A;
(2) adding 333kg of the rest medical stone, 30kg of DL-alpha-tocopherol acetate, a premix A, 180kg of citric acid, 100kg of fumaric acid, 10kg of plant essential oil compound, 20kg of yeast selenium, 30kg of phytase, 8kg of DL-methionine, 150kg of soybean phospholipid oil powder and 100kg of calcium hydrophosphate into a mixer, mixing for 5 minutes, and sieving by a 2mm analysis sieve to obtain the composite premixed feed with the mixing uniformity variation coefficient of 5%.
Example 2
An anti-stress compound premix feed for laying hens is composed of the following raw materials by weight:
the plant essential oil compound is prepared by the following method: mixing 3kg of carvacrol and 3kg of eugenol uniformly by using a homogenizing device, adding 6kg of silicon dioxide to adsorb essential oil, mixing uniformly, and sieving by using a 2mm analysis sieve to obtain 12kg of plant essential oil compound.
The preparation method of the anti-stress compound premix feed for the laying fowl comprises the following steps:
(1) 0.2kg of ammonium sulfate nitrate, 0.5kg of riboflavin, 7kg of nicotinamide, 0.5kg of D-calcium pantothenate, 1.5kg of pyridoxine hydrochloride, 0.5kg of menadione sodium bisulfite, 3kg of biotin and vitamin B12Weighing 0.8kg, 4kg of 25-hydroxycholecalciferol and 1kg of protease according to the weight, adding 10kg of silicon dioxide and 15kg of medical stone, uniformly mixing, and sieving by using a 2mm analysis sieve to obtain premix A;
(2) adding 329kg of the rest medical stone, 30kg of DL-alpha-tocopherol acetate, a premix A, 200kg of citric acid, 80kg of fumaric acid, 12kg of plant essential oil compound, 60kg of yeast selenium, 10kg of phytase, 10kg of DL-methionine, 150kg of soybean phospholipid oil powder and 80kg of calcium hydrophosphate into a mixer, mixing for 10 minutes, and sieving by a 2mm analysis sieve to obtain the composite premixed feed with the mixing uniformity variation coefficient of 3%.
Example 3
An anti-stress compound premix feed for laying hens is composed of the following raw materials by weight:
the plant essential oil compound is prepared by the following method: mixing 1kg of carvacrol and 3kg of eugenol uniformly by using a homogenizing device, adding 4kg of silicon dioxide to adsorb essential oil, mixing uniformly, and sieving by using a 2mm analysis sieve to obtain 8kg of plant essential oil compound.
The preparation method is the same as example 1.
Test example 1: influence of premixed feed on production performance and egg quality of commercial laying hens
1. Design of experiments
1.1 test animals and materials
Selecting a commercial egg laying chicken farm, randomly dividing healthy Hailan brown egg laying chickens 7200 feathers in the later period of egg laying into 2 groups in 380 days of day age, and treating 6 replicates in each group, wherein each replicate is 600 feathers. The test pre-feeding period is 7 days, and the test period is 28 days, which totals 35 days. The test group added the premix feed prepared in example 1 of the present invention in a mass ratio of 0.5% on the basis of the blank group ration. The blank group daily ration is prepared according to the laying hen basic daily ration nutrition level by referring to the laying hen nutrition requirement standard in the American NRC (1994) broiler chicken nutrition standard.
1.2 Breeding management
Feeding places: shandong province chat in a chicken house
Feeding time: 12 months in 2019-2020 and 1 month in
The feeding management carries out immunization and daily management according to the conventional management program of a test field, the test carries out three-layer stacked cage feeding in the same house, 3 chickens are placed in each cage, automatic operation of drinking water and excrement cleaning is carried out, a specially-assigned person is fixed for feeding during the test period, 3 times of feeding are carried out every day, drinking water is taken freely, eggs are picked manually, the eggs are picked 2 times every day, and the production condition of the chicken flocks is observed at any time. The temperature of the colony house is 23-25 ℃ during the feeding period, and the relative humidity is 65%.
1.3 measurement of index
The production performance index is as follows: during the test period, the egg number, egg weight, feed consumption, dead panning number and malformed egg number (soft shell eggs and small eggs) are recorded every day; and (4) calculating the laying rate, the feed-egg ratio, the dirty egg rate and the malformed egg rate (including soft-shell eggs, pocket eggs and the like) at the end of the test.
Laying rate: the number of egg laying/number of stock columns is multiplied by 100 percent;
the dirty egg rate: the number of the dirty eggs/the number of the total produced eggs is multiplied by 100 percent.
The malformed egg rate: the number of malformed eggs/total egg production is multiplied by 100 percent
Material-egg ratio: daily average feed intake/(average egg weight × laying rate).
The quality index of the eggs is as follows: egg weight, egg shape index, eggshell color, eggshell strength, eggshell thickness, yolk color, yolk ratio.
1.4 data analysis
The experimental data were statistically analyzed by ANOVA single factor and Duncan multiple comparison method in statistical analysis textbook SPSS 19 (Chinese edition) statistical analysis practical course, with P < 0.05 as a significant difference.
The test results are shown in tables 1 and 2.
Table 1 effect of premix feed prepared in example 1 on the production performance of layers
Note: malformed egg rate, mainly consisted of pocket-sized and soft-shelled eggs.
As can be seen from table 1, after the premix feed prepared by the embodiment of the invention is added into the daily ration additive, the decrease of the laying rate caused by the oxidative stress of egg laying can be effectively alleviated, the laying rate in the later period of egg laying can be improved by regulating the intestinal health of the laying hens, the feed-egg ratio, the dirty-egg rate and the malformed egg rate can be reduced, the feed-egg ratio of the test group is reduced by 8.33% compared with that of the blank group, the dirty-egg rate is reduced by 39.67% compared with that of the blank group (P is less than or equal to 0.05), and the malformed egg rate is reduced by 62.44% compared with that of the blank group (P is less than or equal to 0.05), which indicates that the premix feed can effectively improve the.
Table 2 effect of premix feed prepared in example 1 on egg quality
Note: and detecting the color of the eggshell by using a QCR-British TSS eggshell color determinator, wherein the specific detection method refers to the specification, and the darker the color is, the smaller the numerical value is.
As can be seen from Table 2, the premix feed prepared in the examples of the present invention had a greater effect on the egg shape index, the color of the eggshell, the color of the yolk and the ratio of the yolk in the daily ration, and the color of the eggshell and the color of the yolk in the test group were significantly darker (P.ltoreq.0.05) than the blank group. The indexes of eggshell strength, eggshell thickness, egg weight and the like have no significant difference between the test group and the blank group (P is more than 0.05).
Test example 2: influence of premix feed on breeding hens productivity and reproductive performance
Design of experiments
1. Test animals and materials
The test was conducted in a commercial chicken house, selecting healthy breeders in the laying period, 450 cocks, 15000 hens, and 410 days old, randomly divided into 2 groups, each treated for 5 replicates, one replicate per floor per side of the chicken house, and 1500 replicates each. The experimental period is 7 days, the experimental period is 28 days, and the total time is 35 days. The test group added the premix feed prepared in example 1 of the present invention in a mass ratio of 0.5% on the basis of the blank group ration. The blank group daily ration is prepared according to the laying hen basic daily ration nutrition level by referring to the laying hen nutrition requirement standard in the American NRC (1994) broiler chicken nutrition standard.
2. Feeding management
Feeding places: a chicken farm prepared from Handan from Hebei
Feeding time: 5-2020 and 6-2020
The feeding management is used for carrying out immunization and daily management according to a conventional management program of a test field, the test is carried out in three-layer stacked cage culture in the same house, 3 chickens are placed in each cage, a specially-assigned person is fixed for feeding during the test, the cage is fed for 3 times every day, drinking water is freely taken, eggs are manually picked up, the eggs are picked up for 2 times every day, and the production condition of the chicken flocks is observed at any time. Hatching eggs are hatched for 1 batch 5-7 days, and hatching data is tracked.
3. Measurement index
The production performance index is as follows: the egg number and the qualified egg number are recorded every day during the test period; and (5) calculating the average egg weight, the laying rate and the qualified egg rate after the experiment is finished.
The reproductive performance index is as follows: fertility rate, hatchability and healthy chick rate were followed.
Fertilization rate: the number of fertilized eggs/hatched eggs is multiplied by 100%
Hatching rate of fertilized eggs: hatching number/fertilized egg number is multiplied by 100%
Healthy chick rate: healthy chick number/total chick number × 100%
And (4) analyzing economic benefits and calculating the input-output ratio.
4. Data analysis
The experimental data were statistically analyzed by ANOVA single factor and Duncan multiple comparison method in statistical analysis textbook SPSS 19 (Chinese edition) statistical analysis practical course, with P < 0.05 as a significant difference.
The test results are shown in tables 3 and 4,
table 3 effect on production performance of premix feed prepared in example 1
As can be seen from Table 3, compared with the blank group, the premix feed prepared by the embodiment of the invention has no adverse effect on the production performance of the breeding hens after being added into the daily ration, the laying rate and the qualified egg rate of the breeding hens in the house are improved to a certain extent, and are respectively improved by 3.05 percent and 1.36 percent compared with the blank group, but the difference is not obvious.
Table 4 effect on reproductive performance of the premix feed prepared in example 1
As can be seen from Table 4, the addition of the premixed feed prepared by the embodiment of the invention into the daily ration significantly improves the reproductive performance of the breeding hens, and compared with the blank group, the fertilization rate of the hatched eggs in the test group is improved by 4.33%, and the hatching rate of the hatched eggs is improved by 4.07%. By referring to the production data, the number of healthy chicks of hatching eggs of the blank group and the test group is respectively 2932 and 3267 feather according to calculation of 5000 hens of the blank group and the test group, the number of healthy chicks of 335 feather of the test group is effectively increased, and the economic benefit is greatly improved.
In conclusion, the anti-stress compound premix feed for laying fowls provided by the invention can effectively improve the production performance and reproductive performance of the laying fowls. Mainly improves the quality of sperms and reduces the oxidative stress damage of eggs by enhancing the antioxidant third-level defense system of the laying fowls; regulating intestinal tract and liver health, promoting digestion, absorption and metabolism of nutrients, improving egg laying performance, and improving immune response capability.
Claims (10)
1. An anti-stress compound premix feed for laying hens is composed of the following raw materials in parts by weight: 160-240 parts of citric acid, 80-160 parts of fumaric acid, 8-15 parts of plant essential oil compound, 2-5 parts of 25-hydroxycholecalciferol, 0.15-0.75 part of ammonium sulfate nitrate, 6-15 parts of nicotinamide, 0.2-0.5 part of riboflavin, 0.2-0.5 part of D-calcium pantothenate, 1-2 parts of pyridoxine hydrochloride, 20-36 parts of DL-alpha-tocopheryl acetate, 0.4-0.7 part of menadione sodium bisulfite, 1-4 parts of biotin, and vitamin B120.3-0.9 part of phytase, 10-30 parts of protease, 1-5 parts of protease, 2-10 parts of DL-methionine, 20-60 parts of yeast selenium, 50-150 parts of calcium hydrophosphate and 200-600 parts of carrier.
2. The anti-stress compound premix feed for laying fowls as claimed in claim 1, wherein the anti-stress compound premix feed for laying fowls is composed of the following raw materials in parts by weight: 170-210 parts of citric acid, 90-150 parts of fumaric acid, 9-13 parts of plant essential oil compound, 2-4 parts of 25-hydroxycholecalciferol, 0.2-0.6 part of ammonium sulfate nitrate, 8-12 parts of nicotinamide, 0.25-0.45 part of riboflavin, 0.25-0.45 part of D-calcium pantothenate, 1.2-2 parts of pyridoxine hydrochloride, 20-32 parts of DL-alpha-tocopheryl acetate, 0.45-0.65 part of menadione sodium bisulfite, 1.2-3.6 parts of biotin, and vitamin B120.35-0.85 part of phytase, 15-30 parts of protease, 2-5 parts of protease, 3-9 parts of DL-methionine, 20-50 parts of yeast selenium, 60-140 parts of calcium hydrophosphate and 250-550 parts of carrier.
3. The anti-stress compound premix feed for laying fowls as claimed in claim 1, wherein the plant essential oil compound is prepared by the following method: uniformly mixing carvacrol and eugenol according to the mass ratio of 2:1, and adding silicon dioxide for adsorption to obtain the plant essential oil compound, wherein the total mass of carvacrol and eugenol is more than 37.5%.
4. The anti-stress compound premix feed for the laying fowl as claimed in claim 3, wherein the carvacrol is synthesized by organic chemical engineering, and the content of effective components is more than or equal to 90%; the eugenol is volatile oil obtained by distilling dry flower bud of flos Caryophylli of Myrtaceae, and is yellowish or colorless clear oily substance with effective component greater than or equal to 90%.
5. The anti-stress compound premix feed for laying poultry according to claim 1, characterized in that the DL-alpha-tocopherol acetate is feed-grade water-soluble DL-alpha-tocopherol acetate powder, and the content is more than or equal to 50%; the product component analysis guaranteed value of the 25-hydroxycholecalciferol is greater than or equal to 0.125%; the biotin is feed-grade biotin, and the content of the biotin is more than or equal to 2%; the vitamin B12Is feed grade vitamin B12The content is more than or equal to 1 percent.
6. The anti-stress composite premix feed for laying fowls according to claim 1, wherein the phytase is derived from aspergillus niger or pichia pastoris, and has an activity of 4000 to 6000 IU/kg; the protease is derived from Aspergillus niger or Bacillus subtilis, and the activity is 40000-60000 IU/kg.
7. The anti-stress compound premix feed for laying fowls as claimed in claim 1, wherein the carrier is one or a mixture of two or more of pine needle powder, soybean lecithin oil powder, medical stone, zeolite powder, stone powder, anhydrous sodium sulphate and silicon dioxide.
8. The method for preparing the anti-stress compound premix feed for the laying fowl as claimed in claim 1, comprising the steps of:
1) mixing ammonium sulfate nitrate, riboflavin, nicotinamide, D-calcium pantothenate, pyridoxine hydrochloride, and methylnaphthalene sodium bisulfiteQuinone, biotin, vitamin B12Mixing 25-hydroxycholecalciferol, protease, DL-methionine and part of carrier, and sieving to form premix A;
2) adding fumaric acid, citric acid, a plant essential oil compound, DL-alpha-tocopherol acetate, phytase, premix A, calcium hydrophosphate, selenium yeast and the rest of carriers into a mixer, mixing for 5-10 minutes, and sieving to obtain the anti-stress composite premixed feed for the eggs and the poultry.
9. The preparation method of claim 8, wherein the step (1) and the step (2) are performed by sieving with a 2-3 mm sieve so that the mixing uniformity coefficient of variation is not more than 5%.
10. The application of the anti-stress compound premix feed for the laying fowl as claimed in any one of claims 1 to 7 in the process of breeding the laying fowl, which is used for resisting stress of the laying fowl, and the specific application method is as follows: the feed additive is added into the feed according to the proportion of 0.5-1.0% of the total mass of the feed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011452817.4A CN112568326A (en) | 2020-12-11 | 2020-12-11 | Anti-stress composite premixed feed for laying fowls and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011452817.4A CN112568326A (en) | 2020-12-11 | 2020-12-11 | Anti-stress composite premixed feed for laying fowls and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112568326A true CN112568326A (en) | 2021-03-30 |
Family
ID=75131337
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011452817.4A Pending CN112568326A (en) | 2020-12-11 | 2020-12-11 | Anti-stress composite premixed feed for laying fowls and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112568326A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113545424A (en) * | 2021-07-29 | 2021-10-26 | 山东省农业科学院畜牧兽医研究所 | Production method of functional rabbit meat product |
| CN113875886A (en) * | 2021-10-18 | 2022-01-04 | 湖南省微生物研究院 | Mixed feed for improving reproductive capacity of livestock and poultry as well as preparation method and application thereof |
| CN115428861A (en) * | 2021-06-03 | 2022-12-06 | 菲吉乐科(中国)生物科技有限公司 | 25-OH-D3-based compound vitamin and preparation process and application thereof |
| WO2023192038A1 (en) * | 2022-03-29 | 2023-10-05 | Can Technologies, Inc. | Nitrate supplement for poultry for improved offspring quality |
| WO2023192041A1 (en) * | 2022-03-29 | 2023-10-05 | Can Technologies, Inc. | Method for improving offspring quality |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102987093A (en) * | 2012-10-29 | 2013-03-27 | 安佑（中国）动物营养研发有限公司 | Feeding plant essential oil additive as well as preparation and application of additive |
| CN106509457A (en) * | 2016-11-08 | 2017-03-22 | 河南普尼尔生物制药有限公司 | Intestinal tract health protection agent capable of reducing stress of weanling pigs as well as preparation method and use method thereof |
| CN106798156A (en) * | 2016-12-28 | 2017-06-06 | 北京市华都峪口禽业有限责任公司 | A kind of laying hen compound premixed feed and its adequate diet |
| CN109077180A (en) * | 2018-07-31 | 2018-12-25 | 广东蔚莱生物科技有限公司 | A kind of composite plant essential oil feed addictive and the preparation method and application thereof |
| CN110122697A (en) * | 2019-04-29 | 2019-08-16 | 北农大科技股份有限公司 | It is a kind of for improving the Egg Production of Laying Hens later period compound premixed feed of eggshell quality |
| CN110800864A (en) * | 2019-12-18 | 2020-02-18 | 河南牧鹤(集团)饲料有限公司 | Premix for prolonging laying period of laying hens |
-
2020
- 2020-12-11 CN CN202011452817.4A patent/CN112568326A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102987093A (en) * | 2012-10-29 | 2013-03-27 | 安佑（中国）动物营养研发有限公司 | Feeding plant essential oil additive as well as preparation and application of additive |
| CN106509457A (en) * | 2016-11-08 | 2017-03-22 | 河南普尼尔生物制药有限公司 | Intestinal tract health protection agent capable of reducing stress of weanling pigs as well as preparation method and use method thereof |
| CN106798156A (en) * | 2016-12-28 | 2017-06-06 | 北京市华都峪口禽业有限责任公司 | A kind of laying hen compound premixed feed and its adequate diet |
| CN109077180A (en) * | 2018-07-31 | 2018-12-25 | 广东蔚莱生物科技有限公司 | A kind of composite plant essential oil feed addictive and the preparation method and application thereof |
| CN110122697A (en) * | 2019-04-29 | 2019-08-16 | 北农大科技股份有限公司 | It is a kind of for improving the Egg Production of Laying Hens later period compound premixed feed of eggshell quality |
| CN110800864A (en) * | 2019-12-18 | 2020-02-18 | 河南牧鹤(集团)饲料有限公司 | Premix for prolonging laying period of laying hens |
Non-Patent Citations (2)
| Title |
|---|
| 佟建明: "《饲料添加剂手册》", 31 May 2001, 中国农业大学出版社 * |
| 徐淑芳: "《青壳蛋鸡》", 29 February 2004, 科学技术文献出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115428861A (en) * | 2021-06-03 | 2022-12-06 | 菲吉乐科(中国)生物科技有限公司 | 25-OH-D3-based compound vitamin and preparation process and application thereof |
| CN113545424A (en) * | 2021-07-29 | 2021-10-26 | 山东省农业科学院畜牧兽医研究所 | Production method of functional rabbit meat product |
| CN113875886A (en) * | 2021-10-18 | 2022-01-04 | 湖南省微生物研究院 | Mixed feed for improving reproductive capacity of livestock and poultry as well as preparation method and application thereof |
| WO2023192038A1 (en) * | 2022-03-29 | 2023-10-05 | Can Technologies, Inc. | Nitrate supplement for poultry for improved offspring quality |
| WO2023192041A1 (en) * | 2022-03-29 | 2023-10-05 | Can Technologies, Inc. | Method for improving offspring quality |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112568326A (en) | Anti-stress composite premixed feed for laying fowls and preparation method and application thereof | |
| Shalash et al. | Evaluation of distillers dried grains with solubles as feed ingredient in laying hen diets | |
| Bouyeh et al. | Influence of different levels of lysine, methionine and protein on the performance of laying hens after peak | |
| CN110353114A (en) | A kind of cow feed additive and its preparation process | |
| CN107821788A (en) | Small molecular organic acid type acid supplement coated slow release formulation | |
| CN111109442A (en) | Compound feed for pregnant sows of Sichuan-Tibet black pigs and using method of compound feed | |
| CN102028112B (en) | 817 broiler breeder feed | |
| CN109769749B (en) | Selenium-rich Beijing fried chicken egg and production method thereof | |
| CN113974014B (en) | High-stability layer chicken nutrition additive | |
| CN102948651A (en) | Breeding goose premix for improving egg hatching rate | |
| KR101740890B1 (en) | Hen feed composition for reducing stress and inhibiting decrease of egg production | |
| Honarbakhsh et al. | Interactive effects of dietary betaine and saline water on carcass traits of broiler chicks | |
| KR100502982B1 (en) | Breeding method of a duck for laying eggs using sulfur and method of producting sulfer duck from the duck | |
| CN113261625A (en) | Premix for laying chicks, compound feed and preparation method thereof | |
| RU2495588C2 (en) | Method for growing rearing stock for parental chicken flock | |
| CN112042822A (en) | Compound feed for improving reproductive performance of sows in lactation period | |
| CN111357886A (en) | Premix for laying hen in initial stage, preparation method thereof, complete feed thereof and application thereof | |
| CN111149918A (en) | Composition of green disease-free yield-increasing cultivation technology and application | |
| Mahdi et al. | EFFICIENCY OF DIFFERENT LEVELS OF L-CARNITINE SUPPLEMENTATION ON GROWTH PERFORMANCE AND BLOOD PARAMETERS IN THREE LINES OF KURDISH QUAILS (COTURNIX COTURNIX) | |
| Uddin et al. | Growth responses, carcass yield characteristics, gastro-intestinal development, viability and profitability of Japanese quail fed on conventional diet supplemented with organic acid | |
| WO2011015651A1 (en) | Use of canthaxanthin compounds | |
| AL-Hashimi et al. | USE OF THE HERBAL METHIONINE AS AN ALTERNATIVE TO SYNTHETIC METHIONINE (DLMETHIONINE) TO IMPROVEMENT THE PERFORMANCE AND FEED COST ECONOMICALLY FOR THE LAYER (ISA BROWN) | |
| Mohammed et al. | Comparison of in ovo injection at 18 days of incubation and feeding in the hatchery and their interaction in influencing on some of hatching and productive traits in broiler chickens | |
| Kumar et al. | Influence of methionine and protein supplementation in corn-soya and wheat-soya based diets on laying hens performance, egg size and egg quality in early stage of egg production | |
| Goma et al. | IMPACT OF ROYAL JELLY, GIBBERELLIC ACID AND TESTOSTERONE TREATMENTS ON SOME PRODUCTIVE, EGG QUALITY TRAITS AND ECONOMIC EFFICIENCY IN LOCAL CHICKEN STRAIN, DURING THE SUMMER SEASON OF EGYPT. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210330 |
|
| RJ01 | Rejection of invention patent application after publication |