CN112545570A - Nucleic acid detection and extraction device and extraction method - Google Patents
Nucleic acid detection and extraction device and extraction method Download PDFInfo
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- CN112545570A CN112545570A CN202011514362.4A CN202011514362A CN112545570A CN 112545570 A CN112545570 A CN 112545570A CN 202011514362 A CN202011514362 A CN 202011514362A CN 112545570 A CN112545570 A CN 112545570A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/0045—Devices for taking samples of body liquids
- A61B10/0051—Devices for taking samples of body liquids for taking saliva or sputum samples
Abstract
The invention discloses a nucleic acid detection and extraction device and an extraction method, and relates to the technical field of nucleic acid detection and extraction. The invention relates to a nucleic acid detection and extraction device and an extraction method, which are convenient for collecting sputum in the oral cavity of a detector and sealing the collected sample for convenient marking and inspection, so that the detection efficiency is high, and cross infection is not easy to occur.
Description
Technical Field
The invention relates to the technical field of nucleic acid detection and extraction, in particular to a nucleic acid detection and extraction device and an extraction method.
Background
The nucleic acid detecting substance is nucleic acid of virus; the nucleic acid detection is to find out whether the respiratory tract sample, blood or feces of the patient has the nucleic acid of the virus invaded from the outside to determine whether the patient is infected by the new coronavirus; therefore, once the nucleic acid is detected to be positive, the virus in the body of a patient can be proved, in the prior art, the mode adopted by most of nucleic acid detection is to detect the virus nucleic acid in sputum and nasopharyngeal swab of a detector to judge whether the human body is infected with the virus, and then the collected sample is marked for inspection, so that the efficiency is low, the possibility of cross infection is easy to occur, and the accuracy of nucleic acid detection is influenced.
Disclosure of Invention
The present invention is directed to a nucleic acid detecting and extracting apparatus and method for solving the above problems.
In order to achieve the purpose, the invention provides the following technical scheme: a nucleic acid detection and extraction device comprises a sampling tube and a sampling mechanism, wherein a containing cavity is formed in one end inside the sampling tube, a thread containing groove is formed in one end, far away from the containing cavity, of the sampling tube, a guide hole for communicating the containing cavity with the thread containing groove is formed between the containing cavity and the thread containing groove, the sampling mechanism comprises a second magnet disc and a guide rod, the second magnet disc is arranged inside the containing cavity in a sliding mode, one end of the guide rod is fixedly connected with the axial side wall of the second magnet disc in an inserting mode, the other end of the guide rod penetrates through the guide hole in a sliding mode and extends into the thread containing groove, a sterile cotton ball capable of being contained in the thread containing groove is fixedly sleeved at the other end of the guide rod in a fixing mode, the sampling mechanism slides inside the containing cavity through the second magnet disc, in addition, the guide rod freely slides and pulls inside the guide hole and can drive the sterile cotton ball to extend out of the thread containing groove, the sputum is conveniently collected from the oral cavity of a detector, and the collected sample is sealed and then conveniently marked for inspection, so that the detection efficiency is high, and cross infection is not easy to occur.
In a further embodiment, accomodate the inside one end lateral wall of keeping away from the guiding hole of cavity and fixedly be equipped with first magnet dish, second magnet dish is the same with first magnet dish relative lateral wall magnetic pole, and is the same with first magnet dish relative lateral wall magnetic pole through with second magnet dish for produce the power of mutual repulsion between second magnet dish and the first magnet dish, can slide second magnet dish along accomodating the cavity is inside, can drive the guide bar and freely slide the pull in the guiding hole is inside, can drive aseptic cotton ball and stretch out the screw thread and accomodate the inslot, be convenient for gather the sputum to the detector oral cavity.
In further embodiment, the opening part that the groove was accomodate to the screw thread is equipped with the protection machanism who is used for avoiding being received cross infection after aseptic cotton ball sampling, accomodates the inslot through protection machanism with aseptic cotton ball top recovery screw thread, then accomodates the inslot with the screw thread and pegs graft, can seal up aseptic cotton ball sampling and deposit, avoids appearing cross infection's phenomenon.
In a further embodiment, protection machanism is including protection base and screw thread barrel, the fixed axial one end that sets up at the protection base of screw thread barrel, the screw thread barrel accomodates the inslot rotation through external screw thread and pegs graft, and the screw thread barrel offers the cavity that is used for accomodating aseptic tampon, cup joints the cavity with the screw thread barrel outside aseptic tampon, then accomodate the inslot rotation with the screw thread barrel through external screw thread and peg graft, can utilize protection machanism to retrieve the screw thread with aseptic tampon top and accomodate the inslot, can seal up aseptic tampon sampling, avoid appearing cross infection's phenomenon.
In a further embodiment, the radial side wall of the protection base is symmetrically provided with two auxiliary rotating blocks, and the auxiliary rotating blocks are arranged to facilitate auxiliary rotation of the protection base so as to facilitate the auxiliary protection mechanism to seal and store a sample.
In a further embodiment, two placing grooves are symmetrically formed in one end of the sampling tube, which is far away from the thread accommodating groove, a thread connecting groove is formed in the central position of one end of the sampling tube, which is far away from the thread accommodating groove, an extending mechanism is arranged at one end of the sampling tube, which is far away from the thread accommodating groove, the extending mechanism comprises two extending rods and a connecting seat, the two extending rods are respectively and symmetrically arranged at the bottom end of the connecting seat, the two extending rods are respectively in sliding insertion connection with the insides of the two placing grooves, the connecting seat is axially provided with a rotating hole, an annular limiting groove is radially arranged inside the rotating hole, a threaded connector is rotatably inserted into the rotating hole, two limiting blocks which are rotatably clamped with the inside of the annular limiting groove are symmetrically arranged on the radial side wall of the threaded connector, and the tip end of the threaded connector is rotatably, and the threaded connection head keeps away from the one end of thread connection groove and is equipped with the rotor plate, through rotating threaded connection head for threaded connection head utilizes the stopper around the annular spacing groove rotation in rotation hole, makes inside the pointed end of threaded connection head rolled out the thread connection groove, can follow standing groove pull extension rod afterwards, thereby extension overall structure's length, so that medical personnel handheld sampling operation that carries on.
In a further embodiment, two arc spacing groove has all been seted up, two to one side lateral wall that the inside top of standing groove is close to each other the rectangle dashpot has all been seted up to extension rod relative lateral wall bottom, the inside of rectangle dashpot is close to 45 elastic driving lever of open-ended one side towards the lopsidedness of threaded connection head, the one end that rectangle dashpot was kept away from at elastic driving lever's most advanced is equipped with the hemisphere stopper, the joint is matchd with arc spacing inslot portion to the hemisphere stopper, and the extension rod passes through the inside joint that matches of hemisphere stopper and arc spacing groove, is convenient for like this with the fixed joint of extension rod at the inside opening part of standing groove, realizes avoiding extension mechanism to break away from the.
In a further embodiment, the outer wall of the sampling tube is provided with a label, the surface of the label is covered with a dustproof film which can be torn at will, and the dustproof film is torn so as to be convenient for the marker pen to be arranged on the label, so that the identity of a detected person cannot be identified.
Preferably, the extraction method based on the nucleic acid detection/extraction device includes the steps of:
a1, the magnetic poles of the opposite side walls of the second magnet disc and the first magnet disc are the same, so that mutual repulsion force is generated between the second magnet disc and the first magnet disc, the second magnet disc can slide along the inside of the containing cavity, the guide rod can be driven to freely slide and pull in the guide hole, the sterile cotton ball can be driven to extend out of the threaded containing groove, and sputum of a detector can be conveniently collected in the oral cavity;
a2, sleeving the hollow cavity of the threaded cylinder outside the sterile cotton ball, and then rotationally inserting the threaded cylinder into the threaded accommodating groove through external threads, so that the sterile cotton ball can be pushed back into the threaded accommodating groove by using a protection mechanism, the sterile cotton ball can be sampled and sealed, and the phenomenon of cross infection is avoided;
a3, through rotating threaded connection head for threaded connection head utilizes the stopper around the annular spacing groove rotation that rotates the hole, makes threaded connection head's most advanced inside the roll-out thread connection groove, can follow standing groove pull extension rod afterwards, thereby extension overall structure's length, so that medical personnel handheld sampling operation that carries on.
Compared with the prior art, the invention has the beneficial effects that:
the invention relates to a nucleic acid detection and extraction device and an extraction method, which are convenient for collecting sputum in the oral cavity of a detector and sealing the collected sample for convenient marking and inspection, so that the detection efficiency is high, and cross infection is not easy to occur.
Drawings
FIG. 1 is an exploded view of the main structure of the present invention;
FIG. 2 is a partial cross-sectional view of the extension mechanism of the present invention;
FIG. 3 is a cross-sectional view of a sampling tube and sampling mechanism of the present invention;
fig. 4 is an enlarged view of the structure of fig. 3 a according to the present invention.
In the figure: 1. a sampling tube; 11. a label strip; 12. a dust-proof film; 13. connecting grooves by screw threads; 14. a receiving cavity; 15. a guide hole; 16. a thread receiving groove; 17. a placement groove; 18. a first magnet disc; 19. an arc-shaped limiting groove; 2. a sampling mechanism; 21. a guide bar; 22. a sterile cotton ball; 23. a second magnet disc; 3. a protection mechanism; 31. protecting the base; 32. a threaded cylinder; 33. an auxiliary turning block; 4. an extension mechanism; 41. a connecting seat; 42. an extension rod; 43. a rotating plate; 44. a threaded connector; 45. a limiting block; 46. a buffer tank; 47. an elastic deflector rod; 48. hemispherical stopper.
Detailed Description
The embodiment of the application solves the problems in the prior art by providing a nucleic acid detection and extraction device and an extraction method; the following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example one
Referring to fig. 1-4, the present embodiment provides a nucleic acid detecting and extracting device and an extracting method, including a sampling tube 1 and a sampling mechanism 2, wherein one end of the sampling tube 1 is provided with a receiving cavity 14, one end of the sampling tube 1 away from the receiving cavity 14 is provided with a threaded receiving slot 16, a guide hole 15 for communicating the receiving cavity 14 with the threaded receiving slot 16 is disposed between the receiving cavity 14 and the threaded receiving slot 16, the sampling mechanism 2 includes a second magnet disc 23 and a guide rod 21, the second magnet disc 23 is slidably disposed in the receiving cavity 14, one end of the guide rod 21 is fixedly inserted into an axial side wall of the second magnet disc 23, the other end of the guide rod 21 slidably penetrates through the guide hole 15 and extends into the threaded receiving slot 16, an aseptic cotton ball 22 capable of being received in the threaded receiving slot 16 is fixedly sleeved on the other end of the guide rod 21, the sampling mechanism 2 slides in the receiving cavity 14 through the second magnet disc 23, in addition guide bar 21 freely slides the pull in the inside of guiding hole 15, can drive aseptic cotton ball 22 and stretch out in the screw thread accomodates groove 16, is convenient for gather the sputum to the person's of examining oral cavity, is convenient for mark the censorship after sealing up the sample after gathering, and detection efficiency is high like this, is difficult for appearing cross infection.
In order to solve the problem of how to automatically extend the aseptic cotton ball 22 out of the thread storage groove 16, a first magnet disc 18 is fixedly arranged on the side wall of one end, far away from the guide hole 15, inside the storage cavity 14, a second magnet disc 23 has the same magnetic pole with the opposite side wall of the first magnet disc 18, and the second magnet disc 23 has the same magnetic pole with the opposite side wall of the first magnet disc 18, so that mutual repulsion force is generated between the second magnet disc 23 and the first magnet disc 18, that is, the second magnet disc 23 can slide along the inside of the storage cavity 14, and the guide rod 21 can be driven to freely slide and pull in the guide hole 15, so that the aseptic cotton ball 22 can be driven to extend out of the thread storage groove 16, and sputum can be conveniently collected in the oral cavity of a detector.
In order to solve the problem of how to seal up the sample after gathering, the opening part of screw thread storage groove 16 is equipped with the protection machanism 3 that is used for avoiding being cross infection after aseptic cotton ball 22 samples, takes in the groove 16 with aseptic cotton ball 22 top return screw thread through protection machanism 3 in, then with the interior grafting of screw thread storage groove 16, can seal up aseptic cotton ball 22 samples, avoids appearing cross infection's phenomenon.
In order to solve then the problem of depositing the sample through protection machanism 3, protection machanism 3 includes protection base 31 and screw cylinder 32, the fixed axial one end that sets up at protection base 31 of screw cylinder 32, screw cylinder 32 accomodates the 16 internal rotations of groove through external screw thread and screw and pegs graft, and screw cylinder 32 offers the cavity that is used for accomodating aseptic cotton ball 22, the cavity through with screw cylinder 32 cup joints at aseptic cotton ball 22 outsidely, then accomodate the groove 16 internal rotations peg graft with screw cylinder 32 through external screw thread and screw, can utilize protection machanism 3 to take in the groove 16 with aseptic cotton ball 22 top back screw thread, can deposit aseptic cotton ball 22 sampling, avoid appearing cross infection's phenomenon.
In order to solve the problem of how to assist the rotation of the protection base 31, the radial side wall of the protection base 31 is symmetrically provided with two auxiliary rotating blocks 33, and the auxiliary rotation of the protection base 31 is facilitated by arranging the auxiliary rotating blocks 33, so that the auxiliary protection mechanism 3 can seal and store samples conveniently.
In order to solve the problem of how to extend the length of the whole structure, two placing grooves 17 are symmetrically arranged at one end of the sampling tube 1 far away from the thread accommodating groove 16, a thread connecting groove 13 is arranged at the central position of one end of the sampling tube 1 far away from the thread accommodating groove 16, an extension mechanism 4 is arranged at one end of the sampling tube 1 far away from the thread accommodating groove 16, the extension mechanism 4 comprises two extension rods 42 and a connecting seat 41, the two extension rods 42 are respectively and symmetrically arranged at the bottom end of the connecting seat 41, the two extension rods 42 are respectively and slidably inserted into the two placing grooves 17, a rotating hole is axially arranged on the connecting seat 41, an annular limiting groove is radially arranged inside the rotating hole, a threaded connector 44 is rotatably inserted into the rotating hole, two limiting blocks 45 which are rotatably clamped with the inside of the annular limiting groove are symmetrically arranged on the radial side wall of the threaded connector 44, the tip end of the threaded connector 44 is rotatably, and threaded connection head 44 is kept away from the one end of thread connection groove 13 and is equipped with rotor plate 43, through rotating threaded connection head 44 for threaded connection head 44 utilizes stopper 45 around the annular spacing groove rotation in rotation hole, makes the pointed end of threaded connection head 44 roll out inside thread connection groove 13, can follow standing groove 17 pull extension rod 42 afterwards, thereby prolongs overall structure's length, so that medical personnel handheld sampling operation.
In order to solve the problem how to avoid extension mechanism 4 to break away from sampling tube 1, arc spacing groove 19 has all been seted up to one side lateral wall that two inside tops of standing groove 17 are close to each other, rectangle buffer slot 46 has all been seted up to two extension rod 42 relative lateral wall bottom, rectangle buffer slot 46's inside is close to 45 elastic deflector rod 47 of open-ended one side towards the lopsidedness of threaded connection head 44, the tip of elastic deflector rod 47 is kept away from the one end of rectangle buffer slot 46 and is equipped with hemisphere stopper 48, hemisphere stopper 48 matches the joint with arc spacing groove 19 is inside, extension rod 42 passes through hemisphere stopper 48 and the inside joint that matches of arc spacing groove 19, be convenient for like this with the fixed joint of extension rod 42 at the inside opening part of standing groove 17, realize avoiding extension mechanism 4 to break away from.
Example two
Referring to fig. 1, a further improvement is made on the basis of embodiment 1:
in order to solve the problem of how to conveniently identify the identity of the detected person, the outer wall of the sampling tube 1 is provided with a label strip 11, the surface of the label strip 11 is covered with a dustproof film 12 which can be torn at will, and the label strip 11 is convenient to pass through a marking pen by tearing the dustproof film 12 so as to avoid the situation that the identity of the detected person cannot be identified.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. A nucleic acid detection and extraction device comprises a sampling tube (1) and a sampling mechanism (2), and is characterized in that: a containing cavity (14) is arranged at one end inside the sampling pipe (1), a thread containing groove (16) is arranged at one end of the sampling pipe (1) far away from the containing cavity (14), a guide hole (15) for communicating the accommodating cavity (14) and the thread accommodating groove (16) is arranged between the accommodating cavity (14) and the thread accommodating groove (16), the sampling mechanism (2) comprises a second magnet disc (23) and a guide rod (21), the second magnet disc (23) is arranged inside the containing cavity (14) in a sliding way, one end of the guide rod (21) is fixedly inserted with the axial side wall of the second magnet disc (23), the other end of the guide rod (21) penetrates through the guide hole (15) in a sliding manner, and extends into the thread accommodating groove (16), and the other end of the guide rod (21) is fixedly sleeved with a sterile cotton ball (22) which can be accommodated in the thread accommodating groove (16).
2. The nucleic acid detecting/extracting device according to claim 1, wherein: a first magnet disc (18) is fixedly arranged on the side wall of one end, far away from the guide hole (15), in the accommodating cavity (14), and the magnetic poles of the second magnet disc (23) and the opposite side wall of the first magnet disc (18) are the same.
3. The nucleic acid detecting/extracting device according to claim 1, wherein: the opening of the thread containing groove (16) is provided with a protection mechanism (3) for avoiding cross infection after sampling of the aseptic cotton ball (22).
4. The nucleic acid detecting/extracting device according to claim 3, wherein: protection machanism (3) are including protection base (31) and screw thread barrel (32), the fixed axial one end that sets up in protection base (31) in screw thread barrel (32), screw thread barrel (32) are accomodate groove (16) internal rotation through external screw thread and screw and are pegged graft, and screw thread barrel (32) offer the cavity that is used for accomodating aseptic cotton ball (22).
5. The nucleic acid detecting/extracting device according to claim 4, wherein: two auxiliary rotating blocks (33) are symmetrically arranged on the radial side wall of the protective base (31).
6. The nucleic acid detecting/extracting device according to claim 1, wherein: the sampling tube is characterized in that two placing grooves (17) are symmetrically formed in one end, far away from the thread accommodating groove (16), of the sampling tube (1), a thread connecting groove (13) is formed in the center position of the end, far away from the thread accommodating groove (16), of the sampling tube (1), an extending mechanism (4) is arranged at one end, far away from the thread accommodating groove (16), of the sampling tube (1), the extending mechanism (4) comprises two extending rods (42) and a connecting seat (41), the two extending rods (42) are symmetrically arranged at the bottom end of the connecting seat (41) respectively, the two extending rods (42) are respectively in sliding insertion connection with the two placing grooves (17) in an inner portion, a rotating hole is axially formed in the connecting seat (41), an annular limiting groove is radially formed in the inner portion of the rotating hole, a threaded connecting head (44) is inserted in the inner portion of the rotating hole, and two limiting blocks (45) which are connected with the inner portion of the annular limiting groove in a rotating, the pointed end of threaded connection head (44) is pegged graft with the inside rotation of thread connection groove (13), and threaded connection head (44) keep away from the one end of thread connection groove (13) and be equipped with rotating plate (43).
7. The nucleic acid detecting/extracting device according to claim 6, wherein: two arc spacing groove (19) have all been seted up, two to one side lateral wall that the inside top of standing groove (17) is close to each other extension rod (42) relative lateral wall bottom has all been seted up rectangle dashpot (46), the inside of rectangle dashpot (46) is close to open-ended one side towards elastic driving lever (47) of 45 of the lopsidedness of threaded connection head (44), the tip of elastic driving lever (47) is kept away from the one end of rectangle dashpot (46) and is equipped with hemisphere stopper (48), hemisphere stopper (48) and arc spacing groove (19) inside matching joint.
8. The nucleic acid detecting/extracting device according to claim 1, wherein: the outer wall of the sampling tube (1) is provided with a label (11), and the surface of the label (11) is covered with a dustproof film (12) which can be torn off at will.
9. The extraction method using the nucleic acid detection/extraction device according to claim 1, comprising the steps of:
a1, the opposite side wall magnetic poles of the second magnet disc (23) and the first magnet disc (18) are the same, so that mutual repulsive force is generated between the second magnet disc (23) and the first magnet disc (18), the second magnet disc (23) can slide along the inside of the accommodating cavity (14), the guide rod (21) can be driven to freely slide and pull in the guide hole (15), the sterile cotton ball (22) can be driven to extend out of the thread accommodating groove (16), and sputum can be conveniently collected in the oral cavity of a detector;
a2, sleeving a hollow cavity of a threaded cylinder (32) outside the sterile cotton ball (22), and then rotationally inserting the threaded cylinder (32) into a thread accommodating groove (16) through external threads, so that the sterile cotton ball (22) can be pushed back into the thread accommodating groove (16) by using a protection mechanism (3), and the sterile cotton ball (22) can be sampled and sealed, thereby avoiding cross infection;
a3, through rotating threaded connection head (44), make threaded connection head (44) utilize stopper (45) around the annular spacing groove rotation that rotates the hole for inside the tip of threaded connection head (44) rotated out thread connection groove (13), can follow later along standing groove (17) pull extension rod (42), thereby prolong overall structure's length, so that medical personnel handheld sampling operation.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114209359A (en) * | 2021-12-30 | 2022-03-22 | 江苏戴格诺思生物技术有限公司 | Nucleic acid detection device |
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| CN114209359A (en) * | 2021-12-30 | 2022-03-22 | 江苏戴格诺思生物技术有限公司 | Nucleic acid detection device |
| CN114209359B (en) * | 2021-12-30 | 2023-08-22 | 江苏戴格诺思生物技术有限公司 | Nucleic acid detection device |
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| Publication number | Publication date |
|---|---|
| CN112545570B (en) | 2021-07-20 |
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