CN112538181A - Preparation method of zinc oxide nanoparticle-fibroin composite antibacterial film - Google Patents
Preparation method of zinc oxide nanoparticle-fibroin composite antibacterial film Download PDFInfo
- Publication number
- CN112538181A CN112538181A CN202011430515.7A CN202011430515A CN112538181A CN 112538181 A CN112538181 A CN 112538181A CN 202011430515 A CN202011430515 A CN 202011430515A CN 112538181 A CN112538181 A CN 112538181A
- Authority
- CN
- China
- Prior art keywords
- film
- zinc oxide
- solution
- composite antibacterial
- heating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
- C08J5/18—Manufacture of films or sheets
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
- A01N25/10—Macromolecular compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/34—Shaped forms, e.g. sheets, not provided for in any other sub-group of this main group
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2389/00—Characterised by the use of proteins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K3/00—Use of inorganic substances as compounding ingredients
- C08K3/18—Oxygen-containing compounds, e.g. metal carbonyls
- C08K3/20—Oxides; Hydroxides
- C08K3/22—Oxides; Hydroxides of metals
- C08K2003/2296—Oxides; Hydroxides of metals of zinc
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K2201/00—Specific properties of additives
- C08K2201/011—Nanostructured additives
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Toxicology (AREA)
- Manufacturing & Machinery (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Inorganic Chemistry (AREA)
- Compositions Of Macromolecular Compounds (AREA)
- Laminated Bodies (AREA)
- Inorganic Compounds Of Heavy Metals (AREA)
Abstract
The invention provides a preparation method of a zinc oxide nanoparticle-fibroin composite antibacterial film, which is to dissolve raw silk fiber in 1 wt% of Na2CO3Heating the solution to obtain a raw silk fiber solution with the weight percent of 20 at the temperature of 90 ℃ for 30 minutes, cooling the solution to room temperature, heating the solution again, repeating the heating and the cooling for three times, and drying the solution at the temperature of 50 ℃ for 6 hours; dissolving the treated raw silk fiber in excessHeating in LiBr solution at 63 deg.C for 4 hr, and dialyzing at room temperature; then centrifuging the dialyzed product at 4 ℃ and 8000r/min for 10 minutes, and reserving the centrifuged supernatant; taking Zn (Ac)2·2H2Dissolving O in the centrifugal supernatant, controlling Zn (Ac)2The concentration is 0.01M; drying in a culture dish, and controlling the thickness of the membrane to be about 80 μm; then sealing the film with 2 wt% NaOH solution and heating the film at 80 ℃ for 1 hour to obtain the zinc oxide nano-particle-fibroin composite antibacterial film. The method is green and simple.
Description
Technical Field
The invention relates to the field of surgical products, in particular to a preparation method of a zinc oxide nanoparticle-fibroin composite antibacterial film.
Background
In the field of medical dressings or packaging at present, natural biological high polymer materials are concerned by people in medical dressings and packaging development in recent years due to green environmental protection, strong plasticity and good biological safety, but all the antibacterial effects of the materials are limited and cannot meet the requirements of sanitation or treatment, and how to maintain the excellent physicochemical properties of the materials and simultaneously strengthen the antibacterial performance is a big problem(1-4). Inorganic nano-materials such as metal oxide nano-particles have excellent catalytic performance, antibacterial effect and stable physicochemical properties, but the use of the inorganic nano-materials is limited due to poor processability, and how to well develop the value of the inorganic nano-materials is a research hotspot(5-8). Therefore, how to develop and apply a film material which is green and environment-friendly, has good biocompatibility and antibacterial effect, and find a simple and green preparation method is still a challenge in antibacterial packaging and medical dressing development.
Reference to the literature
[1]C.Sussman,B.Bates-Jensen,Wound Care a Collaborative Practice Manual for Health ProfessionalLippincott Williams&Wilkins,Philadelphia,USA,2007,pp.293–296.
[2]E.N.Mostow,G.D.Haraway,M.Dalsing,J.P.Hodde,D.King,J.Vasc.Surg.2005,41,837.
[3]A.Veves,P.Sheehan,H.T.Pham,Arch.Surg.2002,137,822.
[4]Y.Qin,J.Appl.Polym.Sci.2006,100,2516.
[5]L.Zhang,W.Yu,C.Han,J.Guo,Q.Zhang,H.Xie,Q.Shao,Z.Sun,Z.Guo,Large Scaled Synthesis of Heterostructured Electrospun TiO2/SnO2 Nanofibers withan Enhanced Photocatalytic Activity.J.Electrochem.Soc.164(2017),H651-H656.
[6]B.Song,T.Wang,H.Sun,Q.Shao,J.Zhao,K.Song,L.Hao,L.Wang,Z.Guo,Two-step hydrothermally synthesized carbon nanodots/WO3 photocatalysts with enhanced photocatalytic performance.Dalton T.46(2017),15769-15777.
[7]Z.Sun,L.Zhang,F.Dang,Y.Liu,Z.Fei,Q.Shao,H.Lin,J.Guo,L.Xiang,N.Yerra,Experimental and Simulation Understanding of Morphology Controlled Barium Titanate Nanoparticles under Co-adsorption of Surfactants.CrystEngComm,19(2017),3288-3298.
[8]K.Sun,P.Xie,Z.Wang,T.Su,Q.Shao,J.Ryu,X.Zhang,J.Guo,A.Shankar,J.Li,R.Fan,D.Cao,Z.Guo,Flexible polydimethylsiloxane/multi-walled carbon nanotubes membranous metacomposites with negative permittivity,Polymer 125(2017),125,50-57.
Disclosure of Invention
The invention aims to provide a preparation method of a zinc oxide nanoparticle-fibroin composite antibacterial film, which aims to solve the problems that the prior art can not meet the requirements of biocompatibility and mechanical property of film materials, and how to prepare the antibacterial film by a green and simple method.
As a class of natural biomaterials, silk fibroin membranes have remarkable properties including excellent mechanical properties, light transmittance, fluid handling capacity, moisture permeability, water resistance, bacterial barrier properties, and biocompatibility. Therefore, the invention uses the biomaterial polymer silk fibroin as the matrix of the membrane, and uses the zinc oxide nano-particles with good antibacterial effect and no toxicity to enhance the antibacterial effect on the basis of using the silk fibroin membrane with the function of bacterial barrier, and the experimental verification ensures the biological safety. The synthetic materials are raw silk fiber, sodium hydroxide, zinc acetate and the like, the reaction related to the invention is carried out in solution and in the environment below 100 ℃, the energy consumption is low, and no environmental pollutants are generated.
In order to achieve the purpose, the invention adopts the following technical scheme:
a preparation method of a zinc oxide nanoparticle-fibroin composite antibacterial film comprises the following steps:
(1) dissolving raw silk fiber in 1 wt% of Na2CO3Adding into the solution to obtain 20 wt% raw silk fiber solution, heating at 90 deg.C for 30 min, cooling to room temperature, heating to 90 deg.C again for 30 minAfter heating at 90 deg.C for 30 min and repeating the cooling cycle three times, drying at 50 deg.C for 6 hr.
(2) The treated fibers were dissolved in an excess of 9.3M LiBr solution and heated at 63 ℃ for 4 hours. Subjecting the obtained liquid to dialysis membrane (M)W8000Da) at room temperature for 2 hours, and repeated 5 times. The dialyzed material was centrifuged at 8000r/min at 4 ℃ for 10 minutes.
(3) Taking Zn (Ac)2·2H2Dissolving O in the centrifuged liquid, controlling Zn (Ac)2The concentration was 0.01M. The solution was added to a petri dish and then placed in an oven to dry at 65 ℃ with a membrane thickness of about 80 μm. And taking out the dried film, sealing the film by using 2 wt% NaOH solution, heating the film at 80 ℃ for 1 hour, and taking out the film (the zinc oxide nano-particles-fibroin composite antibacterial film) for later use.
Furthermore, the zinc oxide nanoparticle-silk fibroin composite antibacterial film has remarkable mechanical property, light transmittance, fluid processing capacity, moisture permeability and antibacterial property.
Compared with the prior art, the invention has the following advantages:
the zinc oxide nanoparticle-silk fibroin composite antibacterial film synthesized by the method has the physical characteristics of excellent mechanical property, light transmittance, fluid processing capacity, moisture permeability and the like; compared with the common mixed distribution method, the adopted novel membrane in-situ nanoparticle synthesis method ensures that the nanoparticles are distributed on the membrane more uniformly, so that the overall performance of the membrane is more stable; compared with a control group, the synthesized zinc oxide nanoparticle-fibroin composite antibacterial film has no obvious difference in cytotoxicity experiment and good biocompatibility. And the antibacterial performance of the ZnO nanoparticle-silk fibroin composite antibacterial film added with the ZnO nanoparticles is obviously improved compared with that of a pure silk fibroin film.
Drawings
FIG. 1 is a pictorial representation of the present invention;
FIG. 2 shows a silk membrane (a) containing Zn (Ac)2The stress-strain curves of the silk fibroin film (b), the zinc oxide nano-particles and the silk fibroin composite antibacterial film (c);
FIG. 3 is a scanning electron microscope and an energy spectrum of an electron microscope according to an embodiment of the present invention;
FIG. 4 is a graph of biocompatibility testing in an example of the present invention;
FIG. 5 is a graph showing the antibacterial property test in the example of the present invention.
Detailed Description
The features and advantages of the present invention will be further understood from the following detailed description taken in conjunction with the accompanying drawings. The examples provided are merely illustrative of the method of the present invention and do not limit the remainder of the disclosure in any way.
The methods not mentioned in the following examples are all conventional experimental methods.
Example 1
Preparation method of zinc oxide nanoparticle-fibroin composite antibacterial film
1. Raw materials: raw silk fibres (Bombyx mori), analytically pure NaOH grade, analytically pure Zn (Ac)2·2H2O, analytically pure grade acetic acid, analytically pure grade Na2CO3，Analytically pure grade lithium bromide, dialysis Membrane (M)W:8000Da)
2. The preparation method comprises the following steps: 100g of raw silk fiber was added to 500mL of 0.02M Na2CO3The solution was heated at 90 ℃ for 30 minutes, cooled to room temperature, heated three times again, and dried at 50 ℃ for 6 hours. The treated fibers were dissolved in an excess of 9.3M LiBr solution and heated at 63 ℃ for 4 hours. Subjecting the obtained liquid to dialysis membrane (M)W8000Da) at room temperature for 2 hours, and repeated 5 times. The dialyzed material was centrifuged at 8000r/min at 4 ℃ for 10 minutes. Taking Zn (Ac)2·2H2Dissolving O in the centrifuged liquid, controlling Zn (Ac)2The concentration was 0.01M. The solution was added to a petri dish and then placed in an oven to dry at 65 ℃ with a membrane thickness of about 80 μm. And taking out the dried film, sealing the film by using 2 wt% NaOH solution, heating the film at 80 ℃ for 1 hour, and taking out the film (the zinc oxide nano-particles-fibroin composite antibacterial film) for later use.
3. And (4) testing the properties: (1) applying different pressures, and testing the strain degree of the zinc oxide nanoparticles-the fibroin composite antibacterial film; simultaneously with the silk fibroin film and Zn (Ac)2The silk fibroin membrane ofThen the obtained product is obtained. (2) Observing the zinc oxide nanoparticle-fibroin composite antibacterial film and the distribution of the nanoparticles under a scanning electron microscope; (3) the zinc oxide nano-particles-fibroin composite antibacterial film is used as a substrate, the substrate and 293T cells are incubated together, and the toxicity of the substrate is detected by an MTT method. (4) In order to verify the antibacterial effect of the zinc oxide nanoparticle-fibroin composite antibacterial film, a surface diffusion plate method is used for carrying out antibacterial experiments on staphylococcus aureus (a) and escherichia coli (b).
4. As a result:
(1) compared with the silk membrane without the ZnO nanoparticles, as shown in FIG. 1, B is the ZnO nanoparticle-silk composite antibacterial membrane, which has good flexibility and uniformity, and is milky white due to the refraction and reflection of light caused by the ZnO nanoparticles.
(2) Fibroin film (a), Zn (Ac) -containing2The stress-strain curves of the silk fibroin film (b) and the zinc oxide nanoparticle-silk fibroin composite antibacterial film (c) indicate that the breaking elongation of the zinc oxide nanoparticle-silk fibroin composite antibacterial film is higher than that of the other two films, and the tensile strength is also remarkably improved. (FIG. 2)
(3) FIG. 3 is an electron microscope scanning picture (a: 2500X; b: 10000X; c: 50000X) of a zinc oxide nanoparticle-fibroin composite antibacterial film under different times, wherein ZnO nanoparticles with the diameter of about 300nm can be formed on the film through a nano in-situ synthesis method, the ZnO nanoparticles are uniformly distributed and have no obvious aggregation phenomenon, a sub-convex structure with the diameter of about 30nm exists on each nano-particle convex through amplification, and a micro-nano binary structure similar to a lotus leaf structure is formed, so that the nano-ZnO composite antibacterial film has a super-hydrophobic effect. The electron microscope energy spectrogram (d) shows that the zinc oxide nanoparticle-fibroin composite antibacterial film contains C, O, Zn elements, and further proves that nZnO exists in the fibroin film, which indicates that the nZnO is successfully combined with the fibroin matrix.
(4) FIG. 4 is a diagram showing the results of incubation of a zinc oxide nanoparticle-silk fibroin composite antibacterial film as a substrate with 293T cells and detection of toxicity by MTT. Wherein, a picture is the proliferation condition of 293T cells on the surface of the zinc oxide nano-particles-fibroin composite antibacterial film, and the picture shows that the cell morphology and the number are normal, and the cell has good proliferation capacity on the zinc oxide nano-particles-fibroin composite antibacterial film; and b, a graph is a cytotoxicity test result of 293T cells in the zinc oxide nanoparticle-silk fibroin composite antibacterial film and a control group after 24, 48 and 72 hours by using an MTT method, the p values of two groups of data are 0.265 and far more than 0.05, and the cell activities of the two groups of cells are not obvious, in addition, the cell activities of the zinc oxide nanoparticle-silk fibroin composite antibacterial film at each time point are all more than 93%, and the zinc oxide nanoparticle-silk fibroin composite antibacterial film is proved to have good biocompatibility.
(5) Fig. 5 shows the results of the antibacterial experiments, wherein (a) is staphylococcus aureus, and (b) is escherichia coli, showing that there is a significant difference in the antibacterial effects of the simple fibroin film and the zinc oxide nanoparticle-fibroin composite antibacterial film, compared to the fibroin film, the zinc oxide nanoparticle-fibroin composite antibacterial film inhibits the proliferation of staphylococcus aureus by 4.0-log at 0.5h, and generates 3.4-log for escherichia coli, greatly inhibits the proliferation of two bacteria at 1h, and completely kills the two bacteria at 2 h. Therefore, the zinc oxide nanoparticle-silk fibroin composite antibacterial film added with the ZnO nanoparticles has a remarkable antibacterial performance improvement effect compared with a pure silk fibroin film.
In conclusion, the zinc oxide nanoparticle-silk fibroin composite antibacterial film synthesized by the method has the physical characteristics of excellent mechanical property, light transmittance, fluid processing capacity, moisture permeability and the like. Compared with the common mixed distribution method, the adopted novel membrane in-situ nanoparticle synthesis method ensures that the nanoparticles are distributed on the membrane more uniformly and the overall performance of the membrane is more stable. Compared with a control group, the synthesized zinc oxide nanoparticle-fibroin composite antibacterial film has no obvious difference in cytotoxicity experiment and good biocompatibility.
Claims (2)
1. A preparation method of a zinc oxide nanoparticle-fibroin composite antibacterial film is characterized by comprising the following steps: comprises the following steps of (a) carrying out,
(1) dissolving raw silk fiber in 1 wt% of Na2CO3Adding into the solution to obtain 20 wt% raw silk fiber solution, heating at 90 deg.C for 30 min, cooling to room temperature, heating again, repeating the above steps for three times, and drying at 50 deg.C6 hours;
(2) dissolving the treated raw silk fiber in an excessive 9.3M LiBr solution, and heating at 63 ℃ for 4 hours; heating the solution with MW8000Da dialysis membrane is dialyzed for 2 hours at room temperature and repeated for 5 times; then centrifuging the dialyzed product at 4 ℃ and 8000r/min for 10 minutes, and reserving the centrifuged supernatant;
(3) taking Zn (Ac)2·2H2Dissolving O in the centrifugal supernatant of the step (2), and controlling Zn (Ac)2The concentration is 0.01M; collecting the extract containing Zn (Ac)2Adding the centrifuged supernatant into a culture dish, and drying at 65 ℃ to control the thickness of the membrane to be about 80 mu m; and taking out the dried film, sealing the film by using 2 wt% NaOH solution, heating the film at 80 ℃ for 1 hour, and taking out the film for later use to obtain the zinc oxide nanoparticle-fibroin composite antibacterial film.
2. The method for preparing the zinc oxide nanoparticle-silk fibroin composite antibacterial film as claimed in claim 1, which is characterized in that: the zinc oxide nanoparticle-fibroin composite antibacterial film has remarkable mechanical property, light transmittance, fluid processing capacity, moisture permeability and antibacterial property.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011430515.7A CN112538181A (en) | 2020-12-07 | 2020-12-07 | Preparation method of zinc oxide nanoparticle-fibroin composite antibacterial film |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011430515.7A CN112538181A (en) | 2020-12-07 | 2020-12-07 | Preparation method of zinc oxide nanoparticle-fibroin composite antibacterial film |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112538181A true CN112538181A (en) | 2021-03-23 |
Family
ID=75019779
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011430515.7A Pending CN112538181A (en) | 2020-12-07 | 2020-12-07 | Preparation method of zinc oxide nanoparticle-fibroin composite antibacterial film |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112538181A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103572507A (en) * | 2012-07-24 | 2014-02-12 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparation method for antibiosis ultraviolet prevention silk fibroin nanofiber membrane |
| CN105435300A (en) * | 2015-12-25 | 2016-03-30 | 重庆理工大学 | Nano-silk-fibroin-containing antibacterial medical dressing |
| CN107089677A (en) * | 2017-06-07 | 2017-08-25 | 首都医科大学宣武医院 | Preparation method of zinc oxide nanoparticles based on silk fibroin biological template regulation |
| CN111453760A (en) * | 2020-04-22 | 2020-07-28 | 安徽锦华氧化锌有限公司 | Preparation process of nano zinc oxide with antibacterial property |
-
2020
- 2020-12-07 CN CN202011430515.7A patent/CN112538181A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103572507A (en) * | 2012-07-24 | 2014-02-12 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparation method for antibiosis ultraviolet prevention silk fibroin nanofiber membrane |
| CN105435300A (en) * | 2015-12-25 | 2016-03-30 | 重庆理工大学 | Nano-silk-fibroin-containing antibacterial medical dressing |
| CN107089677A (en) * | 2017-06-07 | 2017-08-25 | 首都医科大学宣武医院 | Preparation method of zinc oxide nanoparticles based on silk fibroin biological template regulation |
| CN111453760A (en) * | 2020-04-22 | 2020-07-28 | 安徽锦华氧化锌有限公司 | Preparation process of nano zinc oxide with antibacterial property |
Non-Patent Citations (4)
| Title |
|---|
| QIANG LU ET AL.: ""Nanofibrous architecture of silk fibroin scaffolds prepared with a mild self-assembly process"", 《BIOMATERIALS》 * |
| REETU YADAV ET AL.: ""Tailoring of electrical and optical properties of regenerated silk fibroin films with metal oxides"", 《JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS》 * |
| RONGFA GAO ET AL.: ""Gas sensors based on ZnO/silk fibroin film for nitrogen dioxide detection under UV light at room temperature"", 《MATERIALS LETTERS》 * |
| 丁浩 等编: "《纳米抗菌技术》", 31 January 2008, 化学工业出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Song et al. | Antibacterial polyvinyl alcohol/bacterial cellulose/nano-silver hydrogels that effectively promote wound healing | |
| Babu et al. | Silver oxide nanoparticles embedded silk fibroin spuns: Microwave mediated preparation, characterization and their synergistic wound healing and anti-bacterial activity | |
| Yan et al. | Electrospun in-situ hybrid polyurethane/nano-TiO 2 as wound dressings | |
| Zhang et al. | Using in situ dynamic cultures to rapidly biofabricate fabric-reinforced composites of chitosan/bacterial nanocellulose for antibacterial wound dressings | |
| Shao et al. | Synthesis and antimicrobial activity of copper nanoparticle loaded regenerated bacterial cellulose membranes | |
| Feng et al. | Antibiofouling zwitterionic gradational membranes with moisture retention capability and sustained antimicrobial property for chronic wound infection and skin regeneration | |
| Jao et al. | Fabrication and characterization of electrospun silk fibroin/TiO2 nanofibrous mats for wound dressings | |
| Huang et al. | Nanofiber-reinforced bulk hydrogel: Preparation and structural, mechanical, and biological properties | |
| CN110644239B (en) | Antibacterial polylactic acid nanofiber and preparation method and application thereof | |
| Nada et al. | The synthesis and characterization of zinc-containing electrospun chitosan/gelatin derivatives with antibacterial properties | |
| Shi et al. | A skin-like nanostructured membrane for advanced wound dressing | |
| Chen et al. | Antimicrobial polyurethane synthetic leather coating with In-situ generated Nano-TiO 2 | |
| Zhou et al. | Well-defined multifunctional superhydrophobic green nanofiber membrane based-polyurethane with inherent antifouling, antiadhesive and photothermal bactericidal properties and its application in bacteria, living cells and zebra fish | |
| CN110747534A (en) | Antibacterial polysaccharide fiber material and preparation method thereof | |
| Ren et al. | Silk fibroin/chitosan/halloysite composite medical dressing with antibacterial and rapid haemostatic properties | |
| Zheng et al. | An antibacterial hemostatic AuNPs@ corn stalk/chitin composite sponge with shape recovery for promoting wound healing | |
| Lv et al. | PLLA–gelatin composite fiber membranes incorporated with functionalized CeNPs as a sustainable wound dressing substitute promoting skin regeneration and scar remodeling | |
| Liu et al. | Nanofibrous scaffold by cleaner magnetron-sputtering additive manufacturing: A novel biocompatible platform for antibacterial application | |
| CN108299696A (en) | Chitin nano fiber antibacterial rubber gloves and preparation method thereof | |
| Xiao et al. | Controllable coating of zinc oxide on protein-based fibers/fabrics for superior antibacterial performance preserving wearable abilities | |
| Ma et al. | Homogeneous silver nanoparticle loaded polydopamine/polyethyleneimine-coated bacterial cellulose nanofibers for wound dressing | |
| Huang et al. | Preparation of multifunctional wound dressings with composite PVA/PE films | |
| Song et al. | A hydrophilic/hydrophobic janus membrane used as wound dressings with enhanced antibacterial properties | |
| Liu et al. | Fabrication of a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing with rapid healing and antibacterial effect | |
| Yi et al. | Highly hygroscopicity and antioxidant nanofibrous dressing base on alginate for accelerating wound healing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210323 |
|
| RJ01 | Rejection of invention patent application after publication |