CN112525867A - Fluorescent compound micro-flow detector based on tapered single-mode fiber - Google Patents
Fluorescent compound micro-flow detector based on tapered single-mode fiber Download PDFInfo
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- CN112525867A CN112525867A CN201910884355.4A CN201910884355A CN112525867A CN 112525867 A CN112525867 A CN 112525867A CN 201910884355 A CN201910884355 A CN 201910884355A CN 112525867 A CN112525867 A CN 112525867A
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- optical fiber
- tapered
- mode optical
- teflon tube
- fluorescent
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6484—Optical fibres
Abstract
The invention discloses a fluorescent compound micro-flow detector based on a tapered single-mode fiber, which comprises a tapered single-mode fiber, a Teflon tube, a sample inlet, a waste liquid outlet, a single-mode fiber input end and a single-mode fiber output end; the method comprises the following steps of (1) making a single mode fiber into a single mode fiber with a tapered end by using a tapering method, putting the single mode fiber into a Teflon tube, sealing and fixing the two ends of the Teflon tube by using AB glue, and punching the side surface of the Teflon tube by using a femtosecond laser system to obtain a solution inlet and a waste liquid outlet to make a micro-flow detector; when a fluorescent molecular solution flows through the Teflon tube and is excited by ultraviolet light, the emergent fluorescent signal is received by the tapered single-mode optical fiber and is transmitted to the spectrum analyzer, the intensity and the wavelength of the fluorescent signal are analyzed, and the property of the fluorescent molecular solution is detected. The device has the advantages of high signal-to-noise ratio and high sensitivity.
Description
Technical Field
The invention belongs to the technical field of micro-flow detection, and particularly relates to a fluorescent compound micro-flow detector based on a tapered single-mode fiber.
Background
After being excited by ultraviolet light, the compound emits light with a wavelength longer than that of the exciting light, and is called fluorescence; the light absorbed by the compound is called excitation light and the resulting fluorescence is called emission light. The wavelength of fluorescence is always longer than the wavelength of ultraviolet light absorbed by the molecule, usually in the visible range. The nature of fluorescence is closely related to the molecular structure, and molecules with different structures cannot emit fluorescence after being excited. At present, the experimental study of fluorescent compounds by various research institutions is mainly completed by using a fluorescence detector. The disadvantage of this detector is that the system is expensive, bulky, and the biological analyte must fluoresce under selected conditions, and is very sensitive to some interference in the fluorescence measurement.
The optical fiber sensor has the advantages of small volume, electromagnetic interference resistance, strong real-time processing capability, high sensitivity and the like, particularly, the optical fiber material has good biocompatibility, has small damage to living bodies, does not cause rejection reaction, and is suitable for application in biomedical detection, clinical medical diagnosis and the like. The optical fiber can transmit the received fluorescence signal to the optical spectrum analyzer almost without loss, and the closed optical transmission channel of the optical fiber can prevent the fluorescence signal from being influenced by ambient light, so the optical fiber-based fluorescence detection technology has the advantages of high signal-to-noise ratio and high sensitivity. With the development of the optical fiber post-processing technology, the mode field diameter of the tapered optical fiber is in the micron or nanometer level, and the evanescent field of light during transmission in the optical fiber is greatly enhanced, so that the sensitivity and the response speed of the optical fiber sensor are remarkably improved, the size of the optical fiber sensor is reduced, and the optical fiber sensor has more advantages in sensing application.
Disclosure of Invention
Aiming at the defects of high manufacturing cost, large volume, easy interference and the like caused by mainly utilizing a fluorescence detector in the detection based on the fluorescent compound at present, the invention aims to provide the fluorescent compound detector which has the advantages of simple structure, small volume, low cost and less required samples, and has the characteristics of high sensitivity, high signal-to-noise ratio and flexible use in actual detection.
In order to realize the purpose, the technical scheme adopted by the invention is as follows: a fluorescent compound micro-flow detector based on a tapered single-mode fiber comprises the tapered single-mode fiber, a Teflon tube, a solution inlet, a waste liquid outlet, a single-mode fiber input end and a single-mode fiber output end; the method comprises the following steps of processing a single mode fiber into a single mode fiber with a tapered end through fiber tapering, putting the single mode fiber into a Teflon tube, sealing and fixing the two ends of the Teflon tube by using AB glue, and punching the side surface of the Teflon tube by using a femtosecond laser system to obtain a solution inlet and a waste liquid outlet to prepare the micro-flow detector; light emitted by the ultraviolet light source enters the tapered single-mode fiber through the input end of the single-mode fiber and leaks through the evanescent field; the fluorescent solution flows into the Teflon tube from the solution inlet, and after being excited by ultraviolet light, the fluorescent solution emits a fluorescent signal, and then the fluorescent signal is received by the tapered single-mode optical fiber and is transmitted to the spectrum analyzer from the single-mode optical fiber output end, so that the intensity and wavelength of the fluorescent signal are analyzed, and the detection of the property of the fluorescent molecular solution is realized. Because the refractive index of the Teflon tube is lower than that of the fluorescent solution, most of fluorescent signals can be limited in the Teflon tube, and the fluorescent signals can be collected more effectively.
The tapered single-mode optical fiber has the diameter of 35 mu m and the length of 12000 mu m.
The inner diameter of the Teflon tube is 228 microns, the outer diameter is 400 microns, the length is 2cm, and the refractive index is 1.29.
The solution inlet is an inflow hole of the fluorescent molecule solution to be detected, and the diameter of the inflow hole is 30 micrometers.
The waste liquid outlet is an outflow hole of the fluorescent molecular solution to be detected, and the diameter of the outflow hole is 30 micrometers.
The diameter of the fiber core at the input end of the single-mode optical fiber is 10 mu m, and the diameter of the cladding is 125 mu m.
The diameter of the fiber core at the output end of the single-mode optical fiber is 10 micrometers, and the diameter of the cladding is 125 micrometers.
The invention has the beneficial effects that: the tapering single-mode optical fiber is applied to a fluorescent compound micro-flow detection technology, the Teflon tube is used for collecting and conducting fluorescence, an emergent fluorescence field is overlapped with a mode field of an optical fiber transmission mode, the coupling efficiency of the fluorescence and the optical fiber is greatly improved, the detection sensitivity is improved, a novel method with low cost, high signal-to-noise ratio and high sensitivity is provided for the fluorescent compound micro-flow detection technology, and the method has important research significance in the aspects of biomedicine and drug screening.
Drawings
FIG. 1 is a diagram of a microfluidic detector of the present invention.
Claims (7)
1. A fluorescent compound micro-flow detector based on a tapered single-mode fiber is characterized by comprising a tapered single-mode fiber (1), a Teflon tube (2), a solution inlet (3), a waste liquid outlet (4), a single-mode fiber input end (5) and a single-mode fiber output end (6); the method comprises the following steps of processing a single-mode optical fiber into a single-mode optical fiber (1) with a tapered end through optical fiber tapering, putting the single-mode optical fiber into a Teflon tube (2), sealing and fixing two ends of the Teflon tube (2) by using AB glue, and punching the side surface of the Teflon tube (2) by using a femtosecond laser system to obtain a solution inlet (3) and a waste liquid outlet (4) to manufacture the micro-flow detector; light emitted by an ultraviolet light source enters the tapered single-mode fiber (1) through the single-mode fiber input end (5) and leaks through an evanescent field; the fluorescence solution to be detected flows into the Teflon tube (2) from the solution inlet (3), after being excited by ultraviolet light, the fluorescence molecules emit fluorescence signals, the fluorescence signals are received by the tapered single-mode optical fiber (1) and are transmitted to the spectrum analyzer from the single-mode optical fiber output end (6), the intensity and the wavelength of the fluorescence signals are analyzed, and the detection of the properties of the fluorescence molecule solution is realized; after the test is finished, the fluorescent solution flows out from a waste liquid outlet (4); because the refractive index of the Teflon tube (2) is lower than that of the fluorescent solution, most of fluorescent signals can be limited in the Teflon tube (2), more effective collection of the fluorescent signals is realized, and the device has the advantages of high signal-to-noise ratio and high sensitivity.
2. The microfluidic fluorescent compound detector of claim 1, wherein the optical fiber comprises a tapered single mode optical fiber, and the tapered single mode optical fiber comprises: the tapered end of the tapered single-mode optical fiber (1) has the diameter of 35 mu m and the length of 12000 mu m.
3. The microfluidic fluorescent compound detector of claim 1, wherein the optical fiber comprises a tapered single mode optical fiber, and the tapered single mode optical fiber comprises: the inner diameter of the Teflon tube (2) is 228 micrometers, the outer diameter of the Teflon tube is 400 micrometers, the length of the Teflon tube is 2cm, and the refractive index of the Teflon tube is 1.29.
4. The microfluidic fluorescent compound detector of claim 1, wherein the optical fiber comprises a tapered single mode optical fiber, and the tapered single mode optical fiber comprises: the solution inlet (3) is an inflow hole of the fluorescent molecule solution to be detected, and the diameter of the inflow hole is 30 micrometers.
5. The microfluidic fluorescent compound detector of claim 1, wherein the optical fiber comprises a tapered single mode optical fiber, and the tapered single mode optical fiber comprises: the waste liquid outlet (4) is an outflow hole of the fluorescent molecular solution to be detected, and the diameter of the outflow hole is 30 micrometers.
6. The microfluidic fluorescent compound detector of claim 1, wherein the optical fiber comprises a tapered single mode optical fiber, and the tapered single mode optical fiber comprises: the diameter of a fiber core of the single-mode optical fiber input end (5) is 10 mu m, and the diameter of a cladding is 125 mu m.
7. The microfluidic fluorescent compound detector of claim 1, wherein the optical fiber comprises a tapered single mode optical fiber, and the tapered single mode optical fiber comprises: the diameter of a fiber core of the single-mode optical fiber output end (6) is 10 mu m, and the diameter of a cladding is 125 mu m.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN113607688A (en) * | 2021-06-03 | 2021-11-05 | 天津工业大学 | Micro-fluidic refractive index sensor based on double-hole microstructure optical fiber |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113607688A (en) * | 2021-06-03 | 2021-11-05 | 天津工业大学 | Micro-fluidic refractive index sensor based on double-hole microstructure optical fiber |
CN113607688B (en) * | 2021-06-03 | 2024-03-19 | 天津工业大学 | Microfluidic refractive index sensor based on double-hole microstructure optical fiber |
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