CN112514735A - Method for improving growth speed, biomass and yield of agaricus bisporus hyphae - Google Patents
Method for improving growth speed, biomass and yield of agaricus bisporus hyphae Download PDFInfo
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- CN112514735A CN112514735A CN202011269396.1A CN202011269396A CN112514735A CN 112514735 A CN112514735 A CN 112514735A CN 202011269396 A CN202011269396 A CN 202011269396A CN 112514735 A CN112514735 A CN 112514735A
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- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 133
- 241000222519 Agaricus bisporus Species 0.000 title claims abstract description 123
- 238000000034 method Methods 0.000 title claims abstract description 55
- 239000002028 Biomass Substances 0.000 title claims abstract description 44
- 239000000243 solution Substances 0.000 claims abstract description 62
- 239000001509 sodium citrate Substances 0.000 claims abstract description 41
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims abstract description 41
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims abstract description 30
- 239000001632 sodium acetate Substances 0.000 claims abstract description 30
- 235000017281 sodium acetate Nutrition 0.000 claims abstract description 30
- 230000008569 process Effects 0.000 claims description 11
- 238000005507 spraying Methods 0.000 claims description 11
- 238000009630 liquid culture Methods 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 9
- 239000002689 soil Substances 0.000 claims description 7
- 239000008057 potassium phosphate buffer Substances 0.000 claims description 6
- 229910000162 sodium phosphate Inorganic materials 0.000 claims description 5
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 claims description 5
- 239000001488 sodium phosphate Substances 0.000 claims description 4
- 239000012064 sodium phosphate buffer Substances 0.000 claims description 4
- 239000002361 compost Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 238000007747 plating Methods 0.000 claims description 2
- 239000008055 phosphate buffer solution Substances 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 description 22
- 238000011282 treatment Methods 0.000 description 22
- 230000000694 effects Effects 0.000 description 12
- 239000000654 additive Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
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- 238000012258 culturing Methods 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
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- 235000004570 Agaricus campestris Nutrition 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
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- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- 241000222501 Agaricaceae Species 0.000 description 1
- 241000222485 Agaricales Species 0.000 description 1
- 241000222518 Agaricus Species 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
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- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 102000003425 Tyrosinase Human genes 0.000 description 1
- 108060008724 Tyrosinase Proteins 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
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- 150000004676 glycans Chemical class 0.000 description 1
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- 238000009776 industrial production Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- AZQWKYJCGOJGHM-UHFFFAOYSA-N para-benzoquinone Natural products O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
A method for increasing growth speed, biomass and yield of Agaricus bisporus mycelium comprises using sodium citrate solution or sodium acetate solution with predetermined concentration to increase growth speed of Agaricus bisporus mycelium; utilizing a sodium citrate solution or a sodium acetate solution with a preset concentration to increase the biomass of the agaricus bisporus; sodium citrate solution, sodium acetate solution or phosphate buffer solution with predetermined concentration is used to increase the yield of the agaricus bisporus.
Description
Technical Field
The invention relates to the field of agaricus bisporus cultivation, in particular to a method for improving the growth speed, biomass and yield of agaricus bisporus mycelium.
Background
Agaricus bisporus belongs to the subphylum Basidiomycotina, the order Agaricales, the family Agaricaceae, the genus Agaricus, and is the most widely cultivated edible fungus in the world. The agaricus bisporus has fleshy and tender meat and is rich in mannose, trehalose and various amino acids. The agaricus bisporus not only has rich nutrition, delicious taste and low calorie, but also has very high health care value, and is increasingly popular among people of all countries. The quinone compound of polysaccharide contained in Agaricus bisporus can be combined with sulfhydryl group to inhibit DNA synthesis, so it has certain anticancer activity and can inhibit tumor. Tyrosinase contained in Agaricus bisporus can dissolve certain cholesterol, and is helpful for lowering blood pressure.
The growth speed of agaricus bisporus hyphae directly influences the length of a cultivation period, thereby influencing the use efficiency of mushroom houses and the economic benefit of industrial production. The growth and yield of agaricus bisporus fruiting bodies depend on sufficient nutrients and selective fermentation materials. At present, in the industrialized production of agaricus bisporus, the addition of additives into the fungus-growing material is common and becomes an important measure for improving the yield of agaricus bisporus. In other words, adding different substances into the agaricus bisporus fermented material has important significance for improving the production efficiency and economic benefit of agaricus bisporus and promoting the development of the agaricus bisporus industry. Therefore, there is an urgent need to research additives for agaricus bisporus to increase the speed, biomass and yield of agaricus bisporus.
Disclosure of Invention
An advantage of the present invention is that it provides a method for increasing the growth rate, biomass and yield of agaricus bisporus mycelium, wherein an agaricus bisporus additive is used to increase the growth rate, biomass and yield of agaricus bisporus mycelium.
An advantage of the present invention is that it provides a method for increasing the growth rate, biomass and yield of agaricus bisporus mycelium, in which a sodium citrate solution of a predetermined concentration is used to increase the growth rate of agaricus bisporus mycelium.
An advantage of the present invention is that it provides a method for increasing the growth rate, biomass and yield of agaricus bisporus mycelium, in which a sodium acetate solution of a predetermined concentration is used to increase the growth rate of agaricus bisporus mycelium.
An advantage of the present invention is that it provides a method for increasing the growth rate, biomass and yield of agaricus bisporus mycelium, in which a sodium citrate solution of a predetermined concentration is used to increase agaricus bisporus biomass.
An advantage of the present invention is that it provides a method for increasing the growth rate, biomass and yield of agaricus bisporus mycelium, in which a sodium acetate solution of a predetermined concentration is used to increase agaricus bisporus biomass.
An advantage of the present invention is that it provides a method for increasing the growth rate, biomass and yield of agaricus bisporus mycelium, in which a sodium citrate solution of a predetermined concentration is used to increase the yield of agaricus bisporus.
An advantage of the present invention is that it provides a method for increasing the growth rate, biomass and yield of agaricus bisporus mycelium, in which a sodium acetate solution of a predetermined concentration is used to increase the yield of agaricus bisporus.
An advantage of the present invention is that it provides a method for increasing the growth rate, biomass and yield of agaricus bisporus mycelium, in which a phosphate buffer solution of a predetermined concentration is used to increase the yield of agaricus bisporus.
Additional advantages and features of the invention will be set forth in the detailed description which follows and in part will be apparent from the description, or may be learned by practice of the invention as set forth hereinafter.
According to the present invention, which can achieve the foregoing and other objects and advantages, a method for increasing the growth rate of agaricus bisporus hyphae of the present invention includes:
a 0.05% strength sodium citrate solution was added to the agaricus bisporus plate medium.
In the above method, a 0.01 or 0.02% sodium acetate solution is added thereto.
According to the present invention, which can achieve the foregoing and other objects and advantages, a method for increasing the growth rate of agaricus bisporus hyphae of the present invention includes:
0.01 or 0.02% sodium acetate solution is added during the process of plating the agaricus bisporus mycelium.
According to the present invention, which can achieve the foregoing and other objects and advantages, a method for increasing biomass of agaricus bisporus of the present invention includes:
a 0.02% strength sodium citrate solution was added to the agaricus bisporus liquid medium.
In the above method, a 0.01 or 0.05% sodium acetate solution is added thereto.
According to the present invention, which can achieve the foregoing and other objects and advantages, a method for increasing biomass of agaricus bisporus of the present invention includes:
0.01 or 0.05% sodium acetate solution is added during liquid culture of Agaricus bisporus mycelium.
According to the present invention, the foregoing and other objects and advantages are achieved by the present invention, which is a method for increasing the yield of agaricus bisporus, comprising:
and spraying 0.05-0.1% sodium citrate solution on the agaricus bisporus three-time culture material.
According to the present invention, the foregoing and other objects and advantages are achieved by the present invention, which is a method for increasing the yield of agaricus bisporus, comprising:
and spraying a sodium citrate solution with the concentration of 0.1% on the surface of the agaricus bisporus after covering soil.
According to the present invention, the foregoing and other objects and advantages are achieved by the present invention, which is a method for increasing the yield of agaricus bisporus, comprising:
and spraying a 0.01% sodium acetate solution on the compost of the agaricus bisporus after covering soil.
According to the present invention, the foregoing and other objects and advantages are achieved by the present invention, which is a method for increasing the yield of agaricus bisporus, comprising:
and spraying 0.1M sodium phosphate and 0.1M potassium phosphate buffer solution on the agaricus bisporus three-time culture material.
According to the present invention, the foregoing and other objects and advantages are achieved by the present invention, which is a method for increasing the yield of agaricus bisporus, comprising:
and spraying 0.01M or 0.1M sodium phosphate buffer solution on the soil-covered surface of the agaricus bisporus.
Further objects and advantages of the invention will be fully apparent from the ensuing description and drawings.
These and other objects, features and advantages of the present invention will become more fully apparent from the following detailed description, the accompanying drawings and the claims.
Drawings
FIG. 1 is a schematic view showing the growth rate of hyphae treated with sodium citrate of different concentrations in example 1 of the method for increasing the growth rate, biomass and yield of hyphae of Agaricus bisporus according to the present invention.
FIG. 2 is a schematic diagram showing the biomass of hyphae treated with sodium citrate of different concentrations in example 2 of the method for increasing the growth rate, biomass and yield of hyphae of Agaricus bisporus according to the present invention.
FIG. 3 is a schematic diagram showing the effect of sodium citrate treatment with different concentrations on mushroom yield in example 3 of the method for increasing the growth rate, biomass and yield of Agaricus bisporus mycelium according to the present invention.
FIG. 4 is a graph showing the yield of mushrooms treated with sodium citrate of different concentrations in example 3 of the method for increasing the hypha growth rate, biomass and yield of Agaricus bisporus according to the present invention.
FIG. 5 is a schematic diagram showing the growth rate of hyphae treated with sodium acetate of different concentrations in example 4 of the method for increasing the growth rate, biomass and yield of hyphae of Agaricus bisporus according to the present invention.
FIG. 6 is a schematic diagram showing the biomass of hyphae treated with sodium acetate of different concentrations in example 5 of the method for increasing the growth rate, biomass and yield of hyphae of Agaricus bisporus according to the present invention.
FIG. 7 is a graph showing the yield of Agaricus campestris treated with different concentrations of sodium acetate in example 6 of the method for increasing the growth rate, biomass and yield of Agaricus bisporus mycelium according to the present invention.
FIG. 8 is a graph showing the effect of different phosphate buffer treatments on mushroom yield in example 7 of the method for increasing the growth rate, biomass and yield of Agaricus bisporus hyphae according to the present invention.
FIG. 9 is a graph showing the yield of Agaricus campestris treated with phosphate buffer solutions of different concentrations in example 7 according to the method for increasing the growth rate, biomass and yield of Agaricus bisporus mycelium of the present invention.
Detailed Description
The following description is presented to disclose the invention so as to enable any person skilled in the art to practice the invention. The preferred embodiments in the following description are given by way of example only, and other obvious variations will occur to those skilled in the art. The basic principles of the invention, as defined in the following description, may be applied to other embodiments, variations, modifications, equivalents, and other technical solutions without departing from the spirit and scope of the invention.
It will be understood by those skilled in the art that in the present disclosure, the terms "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in an orientation or positional relationship indicated in the drawings for ease of description and simplicity of description, and do not indicate or imply that the referenced devices or components must be constructed and operated in a particular orientation and thus are not to be considered limiting.
It is understood that the terms "a" and "an" should be interpreted as meaning that a number of one element or element is one in one embodiment, while a number of other elements is one in another embodiment, and the terms "a" and "an" should not be interpreted as limiting the number.
The method of the invention is applied to industrial cultivation from research in laboratories, and lays a foundation for exploring commercial products of agaricus bisporus additives.
The invention will be further described and illustrated with reference to specific embodiments.
Examples 1,
The sodium citrate solution is added in the process of culturing the agaricus bisporus mycelium so as to improve the growth speed of the agaricus bisporus mycelium.
Further, 0.05% sodium citrate solution was added to the A15 strain plate medium to increase the growth rate of Agaricus bisporus mycelium.
The effect of different concentrations of sodium citrate solutions on the growth rate of agaricus bisporus hyphae can be seen in fig. 1.
In this example, the difference between two streaks was taken after 17 days of plate culture to determine the growth rate of hyphae, which indicated that the growth rate of hyphae was first rapid and then slow as the concentration of sodium citrate solution increased. Wherein the hypha treated by the sodium citrate solution with the concentration of 0.05% grows fast, has significant difference with a control and has the best treatment effect. Therefore, it can be understood that the growth rate of the agaricus bisporus mycelium is influenced by adding sodium citrate solutions with different concentrations in the process of culturing the agaricus bisporus mycelium on a plate, wherein the sodium citrate solution with the concentration of 0.05 percent can improve the growth rate of the agaricus bisporus mycelium most.
Examples 2,
Adding sodium citrate solution in the process of liquid culture of agaricus bisporus mycelium to improve the biomass of the agaricus bisporus mycelium.
Further, 0.02% sodium citrate solution was added to the liquid culture medium of A15 strain to increase the biomass of Agaricus bisporus mycelium.
The effect of different concentrations of sodium citrate solutions on the biomass of agaricus bisporus hyphae can be seen in fig. 2.
In this example, the result of hypha biomass measurement after 17 days of liquid culture shows that hypha biomass accumulation is high first and low later along with the increase of sodium citrate concentration, wherein the treatment of 0.02% sodium citrate is significantly different from the control, and thus hypha biomass accumulation is high and the treatment effect is the best. In other words, during the process of liquid culture of agaricus bisporus mycelium, the growth rate of the agaricus bisporus mycelium is influenced by adding sodium citrate solutions with different concentrations, wherein the sodium citrate solution with the concentration of 0.02 percent can improve the biomass of the agaricus bisporus mycelium most.
Examples 3,
The sodium citrate solution is added in the process of cultivating the agaricus bisporus so as to improve the yield of agaricus bisporus hyphae.
A. The sodium citrate used in this example at different concentrations will affect the yield of agaricus bisporus to different degrees, and the results are shown in fig. 3 below.
In the embodiment, a cultivation test of sodium citrate related concentration is designed by taking the hypha plate culture and liquid culture results as reference, namely sodium citrate solutions with different concentrations are sprayed in the three-time culture materials of the agaricus bisporus for treatment. Statistics of the picking yield of the three seasons of 19 days shows that the yield of 0.05 and 0.10 percent of the sodium citrate solution treatment is higher than that of the control, wherein the mushroom yield is improved by 11.8 percent by 0.05 percent treatment, and the mushroom yield is mainly shown in that the first tide and the third tide are higher than that of the control.
B. In this example, the application of high and low concentrations of sodium citrate will affect the yield of Agaricus bisporus to different degrees, and the results are shown in FIG. 4 below.
And (3) spraying sodium citrate solutions with different concentrations on the surface of the agaricus bisporus after covering soil, and observing whether the influence on the yield is caused. Statistics of the total yield after 40 days of casing showed that both treatments with sodium citrate at different concentrations increased the yield and significantly increased the yield of agaricus bisporus during the first tide, wherein the application of 0.1% sodium citrate increased the mushroom yield by 21.6%.
Examples 4,
Sodium acetate solution is added in the process of culturing the agaricus bisporus mycelium by a flat plate so as to improve the growth speed of the agaricus bisporus mycelium.
The effect of different concentrations of sodium acetate solution on the growth rate of agaricus bisporus hyphae can be seen in fig. 5.
In this example, the results of measuring the growth rate of hyphae after 13 days of plate culture show that the sodium acetate treatment of 0.01% and 0.02% has significant difference from the control, i.e. the hyphae grow fast and the treatment effect is the best.
Examples 5,
Adding sodium acetate solution in the process of liquid culture of agaricus bisporus mycelium to improve the biomass of agaricus bisporus mycelium.
The effect of different concentrations of sodium acetate solutions on the biomass of agaricus bisporus hyphae can be seen in fig. 6.
The result of measuring the hypha biomass in 17 days of liquid culture shows that the treatment of 0.01 and 0.05 percent sodium acetate has significant difference with the control, the hypha biomass is more, and the treatment effect is better.
Examples 6,
Sodium acetate solution is added in the process of cultivating the agaricus bisporus so as to improve the yield of the agaricus bisporus.
The sodium acetate solutions of different concentrations used in this example affected the yield of Agaricus bisporus to different degrees, and the results are shown in FIG. 7 below.
In the embodiment, the culture material after the earthing is processed by the sodium acetate solution with different concentrations is designed by taking the test results of plate culture and liquid culture as reference, and the yield change is observed. The statistics of the total yield after 40 days of covering soil show that the yield can be improved by the treatment of low-concentration sodium acetate, namely the yield of mushrooms is improved by 17.4% by the treatment of 0.01% sodium acetate, and the yield of first-tide mushrooms is mainly improved.
Example 7,
Adding phosphate buffer solution in the process of cultivating the agaricus bisporus so as to improve the yield of the agaricus bisporus.
a. The treatment with 0.1M sodium phosphate and 0.1M potassium phosphate buffer had an effect on the yield of Agaricus bisporus, the results of which are shown in FIG. 8 below.
By referring to the test results of relative concentration of potassium phosphate buffer solution in the early stage of a laboratory, different phosphate buffer solutions are sprayed in the agaricus bisporus three-time culture medium for treatment, and the yield change is observed. The results show that the yields of the 0.1M sodium phosphate and 0.1M potassium phosphate buffer treatment are higher than the control, and significant differences exist, mainly manifested in that the yields of the second tide and the third tide are obviously higher than the control.
b. The treatment with high and low concentrations of sodium phosphate and potassium phosphate buffer had an effect on the yield of Agaricus bisporus, and the results are shown in FIG. 9 below.
Meanwhile, the surface of the agaricus bisporus covered with soil is sprayed with phosphate buffer solutions with different concentrations for treatment, and whether the influence on the yield is caused or not is observed. The results show that the yield was higher in the first tide than in the control for the 4 treatments, where the 0.01M, 0.1M sodium phosphate buffer treatment increased the mushroom yield by 3.3%, 9.6%.
It will be appreciated by persons skilled in the art that the embodiments of the invention described above and shown in the drawings are given by way of example only and are not limiting of the invention.
The objects of the invention have been fully and effectively accomplished. The functional and structural principles of the present invention have been shown and described in the examples, and any variations or modifications of the embodiments of the present invention may be made without departing from the principles.
Claims (11)
1. A method for increasing the growth rate of agaricus bisporus hyphae is characterized by comprising the following steps:
a 0.05% strength sodium citrate solution was added to the agaricus bisporus plate medium.
2. The method for increasing the growth rate of hyphae of agaricus bisporus according to claim 1, wherein 0.01 or 0.02% sodium acetate solution is added thereto.
3. A method for increasing the growth rate of agaricus bisporus hyphae is characterized by comprising the following steps:
0.01 or 0.02% sodium acetate solution is added during the process of plating the agaricus bisporus mycelium.
4. A method for increasing biomass of Agaricus bisporus, comprising:
a 0.02% strength sodium citrate solution was added to the agaricus bisporus liquid medium.
5. The method for increasing the biomass of agaricus bisporus according to claim 4, wherein 0.01 or 0.05% sodium acetate solution is added thereto.
6. A method for increasing biomass of Agaricus bisporus, comprising:
0.01 or 0.05% sodium acetate solution is added during liquid culture of Agaricus bisporus mycelium.
7. A method for increasing the yield of Agaricus bisporus, comprising:
and spraying 0.05-0.1% sodium citrate solution on the agaricus bisporus three-time culture material.
8. A method for increasing the yield of Agaricus bisporus, comprising:
and spraying a sodium citrate solution with the concentration of 0.1% on the surface of the agaricus bisporus after covering soil.
9. A method for increasing the yield of Agaricus bisporus, comprising:
and spraying a 0.01% sodium acetate solution on the compost of the agaricus bisporus after covering soil.
10. A method for increasing the yield of Agaricus bisporus, comprising:
and spraying 0.1M sodium phosphate and 0.1M potassium phosphate buffer solution on the agaricus bisporus three-time culture material.
11. A method for increasing the yield of Agaricus bisporus, comprising:
and spraying 0.01M or 0.1M sodium phosphate buffer solution on the soil-covered surface of the agaricus bisporus.
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