CN112505234B - Microbial degradation device for patulin - Google Patents
Microbial degradation device for patulin Download PDFInfo
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- CN112505234B CN112505234B CN202011305460.7A CN202011305460A CN112505234B CN 112505234 B CN112505234 B CN 112505234B CN 202011305460 A CN202011305460 A CN 202011305460A CN 112505234 B CN112505234 B CN 112505234B
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- side wall
- fixedly connected
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- patulin
- tank body
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/087—Cleaning containers, e.g. tanks by methods involving the use of tools, e.g. brushes, scrapers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
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Abstract
The invention discloses a microbial degradation device for patulin, which comprises a tank body, wherein a first feeding pipe is fixedly connected to the side wall of the tank body in a penetrating manner, a second feeding pipe is fixedly connected to the side wall of the tank body in a penetrating manner, a one-way mechanism is fixedly connected to the inner side wall of the second feeding pipe, and a connecting plate is fixedly connected to the inner side wall of the tank body. In addition, the inner side wall of the tank body is cleaned, so that the next test of personnel is facilitated, the stability of the reagent tube is higher through the clamping and fixing mechanism, the trouble of the tester caused by the overturning of the reagent tube when the reagent enters the reagent tube is prevented, the time is saved, and the test efficiency is improved.
Description
Technical Field
The invention relates to the technical field of microbial degradation, in particular to a microbial degradation device for patulin.
Background
Patulin, which is a main pathogenic bacterium after fruit decay, is fungomycin secreted by penicillium and aspergillus during infection, and is considered as a potential antibiotic when people know the patulin, however, with the intensive research of people, a plurality of toxicological effects of the patulin are gradually discovered, and the patulin can not only cause acute diseases of human bodies, but also cause chronic diseases and cell diseases. It can cause excessive loss of potassium ions by changing the permeability of cell membranes, thereby causing cell loss activity, firstly destroying intestinal tracts after entering the human body, further activating immune systems, causing pathological changes and other inflammations of the intestinal tracts, and more seriously possibly damaging livers and kidneys.
According to the toxicity, the food additive joint expert committee under the food and agriculture organization and the world health organization of the United nations determines that the intake of the human body is 0.4 mug/kg per day, and due to the concern on the health of the human body, particularly for children, the content of the patulin in the fruit juice is controlled to be 20-50 mug/L by relevant regulations of a plurality of countries, and the upper limit of the content of the patulin in the food is regulated to be 50 mug/kg by the world health organization.
Traditional exhibition is microbial degradation device for penicillin, can't accomplish when degrading the exhibition penicillin to the inside clearance of device, this can make the next time microbial degradation to the exhibition penicillin bring the trouble for test data does not have practical meaning, for this reason we propose a exhibition is microbial degradation device for penicillin and solve above-mentioned problem.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a microbial degradation device for patulin.
In order to achieve the purpose, the invention adopts the following technical scheme:
a microbial degradation device for patulin comprises a tank body, wherein a first feeding pipe is fixedly connected to the side wall of the tank body in a penetrating manner, a second feeding pipe is fixedly connected to the side wall of the tank body in a penetrating manner, a one-way mechanism is fixedly connected to the inner side wall of the second feeding pipe, a connecting plate is fixedly connected to the inner side wall of the tank body, a liquid discharge pipe is fixedly connected to the side wall of the connecting plate in a penetrating manner, an electromagnetic valve is fixedly connected to the inner side wall of the liquid discharge pipe, a fixing plate is fixedly connected to the inner side wall of the tank body and positioned below the connecting plate, a placing block is slidably connected to the side wall of the fixing plate in a penetrating manner, a placing groove is formed in the top of the placing block, a reagent pipe is tightly attached to the inner side wall of the placing groove, a clamping and fixing mechanism is formed in the side wall of the placing block, a second reset spring is fixedly connected to the bottom of the placing block, and the bottom of the second reset spring is fixedly connected with the bottom of the inner side wall of the tank body, the utility model discloses a glass cleaning machine, including the jar body, the lateral wall fixedly connected with glass mirror surface of the jar body, the lateral wall sliding connection of the jar body has the push-and-pull door, the push-and-pull door is located the intermediate position of connecting plate and fixed plate, the lateral wall fixedly connected with handle of push-and-pull door, sliding connection has the slide bar at the top of the jar body through sliding connection, the bottom fixedly connected with extrusion clearance mechanism of slide bar, the top fixedly connected with handle of slide bar.
Preferably, the one-way mechanism includes the magnet piece with second filling tube inside wall fixed connection, the lateral wall of magnet piece closely laminates there is the closing plate, the tip fixedly connected with pivot of closing plate, the inside wall fixedly connected with torsion spring of pivot, the lateral wall of pivot is rotated with the inside wall of second filling tube and is connected, torsion spring's tip and the inside fixed connection of closing plate, torsion spring's tip and the inside fixed connection of second filling tube, the lateral wall of closing plate and the inside wall sealing sliding connection of second filling tube.
Preferably, press from both sides tight fixed establishment including placing the constant head tank that the piece lateral wall was seted up, the inside wall of constant head tank closely laminates there is the locating piece, the first reset spring of tip fixedly connected with of locating piece, first reset spring's tip fixedly connected with circular slot, the circular slot is seted up in the inside of fixed plate, the lateral wall of locating piece runs through sliding connection with the lateral wall of fixed plate.
Preferably, the extrusion clearance mechanism include with slide bar bottom fixed connection's extrusion clearance board, the lateral wall of extrusion clearance board runs through and has seted up the through-hole, the lateral wall fixedly connected with brush hair of extrusion clearance board, the tip of brush hair closely laminates with the inside wall of the jar body.
Preferably, first filling tube and second filling tube set up for the symmetry, the fluid-discharge tube sets up for the array, the quantity of fluid-discharge tube is three, the solenoid valve sets up for the array, the quantity of solenoid valve is three, it sets up for the array to place the piece, the quantity of placing the piece is three, reagent pipe sets up for the array, the quantity of reagent pipe is three, the push-and-pull door sets up for the symmetry, the quantity of push-and-pull door is two, the handle sets up for the symmetry, the quantity of handle is two, second reset spring sets up for the array, second reset spring's quantity is three.
Preferably, the bottom of first filling tube sets up for the slope, the bottom of second filling tube sets up for the slope, the inside wall bottom of standing groove sets up to the hemisphere.
Preferably, the outer diameter of the sealing plate is smaller than the inner diameter of the second feeding pipe, and the outer side wall of the sealing plate is matched with the inner side wall of the second feeding pipe.
Preferably, the first return spring is made of brass, and the first return spring is in a compressed state.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, through the matching use of the positioning block, the positioning groove, the first return spring and the placing block, when the reagent tube is placed on the inner side wall of the placing groove, the reagent tube is manually pressed downwards, so that the outer side wall of the positioning block enters the inner side wall of the fixed outer groove formed in the outer side wall of the placing groove under the action of the first return spring, the placing block is fixed, the reagent tube is prevented from turning over when the reagent is taken, and the placing stability of the reagent tube is improved.
2. According to the invention, through the matching use of the magnet block, the torsion spring, the sealing plate and the rotating shaft, when the solution is added through the feeding pipe, the sealing plate cannot be tightly attached to the magnet block due to the extrusion effect of the solution, when the solution is completely added, the sealing plate is attached to the magnet block again due to the torsion effect of the torsion spring, the sealing of the feeding pipe is completed, so that the solution is prevented from entering from the outside during mixing, the degradation process of the patulin is influenced, and the degradation stability is improved.
3. According to the invention, through the matched use of the slide rod, the handle, the extrusion cleaning plate, the through hole and the brush, when the device is filled with liquid, the handle is pressed downwards to enable the slide rod to slide downwards along the tank body, so that the extrusion cleaning plate extrudes the mixed solution, the solution is mixed more fully, the cleaning of the inner side wall of the tank body by the brush hair is realized, the cleanliness of the inner side wall of the tank body is improved, and the convenience is brought to the next use of the device.
In summary, the following steps: according to the invention, the feeding pipe is opened and sealed, so that when liquid in the tank body is mixed, external bacteria are prevented from entering, a better test result can be obtained for a tester, the liquid in the tank body is more fully mixed by using the extrusion cleaning plate, the degradation speed of patulin is higher, and the test time is saved.
Drawings
FIG. 1 is a schematic front perspective view of a microbial degradation apparatus for patulin according to the present invention;
FIG. 2 is a schematic sectional view of the front side of a microbial degradation apparatus for patulin according to the present invention;
FIG. 3 is an enlarged schematic structural view of the part A in FIG. 2 of the microbial degradation apparatus for patulin provided in the present invention;
FIG. 4 is an enlarged schematic structural view of the part B in FIG. 2 of the microbial degradation apparatus for patulin provided in the present invention;
FIG. 5 is an enlarged schematic structural view of the part C in FIG. 4 of the microbial degradation apparatus for patulin according to the present invention;
fig. 6 is a schematic bottom-up structural view of an extrusion cleaning mechanism of a microbial degradation device for patulin provided by the present invention.
In the figure: 1 tank body, 2 first feed pipes, 3 second feed pipes, 4 connecting plates, 5 liquid discharge pipes, 6 electromagnetic valves, 7 fixing plates, 8 placing blocks, 9 reagent pipes, 10 glass mirror surfaces, 11 sliding doors, 12 handles, 13 sliding rods, 14 handles, 15 extrusion cleaning plates, 16 through holes, 17 bristles, 18 circular grooves, 19 first return springs, 20 positioning blocks, 21 positioning grooves, 22 magnet blocks, 23 sealing plates, 24 rotating shafts, 25 torsion springs and 26 second return springs.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1-6, a microbial degradation device for patulin, comprising a tank body 1, a first feed pipe 2 fixedly connected to the side wall of the tank body 1 through penetration, a second feed pipe 3 fixedly connected to the side wall of the tank body 1 through penetration, a one-way mechanism fixedly connected to the inner side wall of the second feed pipe 3, the one-way mechanism comprising a magnet block 22 fixedly connected to the inner side wall of the second feed pipe 3, a sealing plate 23 tightly attached to the outer side wall of the magnet block 22, the outer diameter of the sealing plate 23 is smaller than the inner diameter of the second feed pipe 3, the outer side wall of the sealing plate 23 is fitted to the inner side wall of the second feed pipe 3, a rotating shaft 24 is fixedly connected to the end of the sealing plate 23, a torsion spring 25 is fixedly connected to the inner side wall of the rotating shaft 24, the outer side wall of the rotating shaft 24 is rotatably connected to the inner side wall of the second feed pipe 3, the end of the torsion spring 25 is fixedly connected to the inside of the sealing plate 23, and the end of the torsion spring 25 is fixedly connected to the inside of the second feed pipe 3, the outer side wall of the sealing plate 23 is in sealing sliding connection with the inner side wall of the second feeding pipe 3, the feeding pipe is opened when liquid is added, the feeding pipe is sealed again by the sealing plate 23 due to the torsion action of the torsion spring 25, and the influence on the price reduction process caused by the entering of external bacteria during the degradation of patulin is prevented;
the inner side wall of the tank body 1 is fixedly connected with a connecting plate 4, the side wall of the connecting plate 4 is fixedly connected with a liquid discharge pipe 5 in a penetrating way, the inner side wall of the liquid discharge pipe 5 is fixedly connected with an electromagnetic valve 6, the inner side wall of the tank body 1 is fixedly connected with a fixing plate 7, the fixing plate 7 is positioned below the connecting plate 4, the side wall of the fixing plate 7 is connected with a placing block 8 in a penetrating way in a sliding way, the top of the placing block 8 is provided with a placing groove, the bottom of the first charging pipe 2 is obliquely arranged, the bottom of the second charging pipe 3 is obliquely arranged, the bottom of the inner side wall of the placing groove is hemispherical, the inner side wall of the placing groove is tightly attached with a reagent pipe 9, the side wall of the placing block 8 is provided with a clamping and fixing mechanism, the clamping and fixing mechanism comprises a positioning groove 21 arranged on the outer side wall of the placing block 8, the inner side wall of the positioning groove 21 is tightly attached with a positioning block 20, and the end part of the positioning block 20 is fixedly connected with a first reset spring 19, the first return spring 19 is made of brass, the first return spring 19 is in a compressed state, the end part of the first return spring 19 is fixedly connected with the circular groove 18, the circular groove 18 is arranged in the fixing plate 7, the outer side wall of the positioning block 20 is in penetrating sliding connection with the side wall of the fixing plate 7, through the steps, clamping and fixing of the reagent tube 9 are achieved, unnecessary troubles brought to a tester by overturning of the reagent tube 9 in the liquid outlet process are prevented, and the stability of the reagent tube 9 is improved;
the bottom of the placing block 8 is fixedly connected with a second reset spring 26, the first feeding pipe 2 and the second feeding pipe 3 are symmetrically arranged, the liquid discharging pipes 5 are arranged in an array, the number of the liquid discharging pipes 5 is three, the number of the electromagnetic valves 6 is arranged in an array, the number of the electromagnetic valves 6 is three, the placing block 8 is arranged in an array, the number of the placing block 8 is three, the number of the reagent pipes 9 is arranged in an array, the number of the reagent pipes 9 is three, the number of the sliding doors 11 is symmetrically arranged, the number of the sliding doors 11 is two, the number of the handles 12 is symmetrically arranged, the number of the handles 12 is two, the second reset spring 26 is arranged in an array, the number of the second reset spring 26 is three, the bottom of the second reset spring 26 is fixedly connected with the bottom of the inner side wall of the tank body 1, the side wall of the tank body 1 is fixedly connected with a glass mirror 10, the side wall of the tank body 1 is slidably connected with the sliding doors 11, and the sliding doors 11 are positioned in the middle positions of the connecting plate 4 and the fixing plate 7, the outer side wall of the sliding door 11 is fixedly connected with a handle 12, the top of the tank body 1 is connected with a sliding rod 13 in a penetrating and sliding manner, the bottom of the sliding rod 13 is fixedly connected with an extrusion cleaning mechanism, the extrusion cleaning mechanism comprises an extrusion cleaning plate 15 fixedly connected with the bottom of the sliding rod 13, a through hole 16 is formed in the side wall of the extrusion cleaning plate 15 in a penetrating manner, bristles 17 are fixedly connected with the outer side wall of the extrusion cleaning plate 15, the end parts of the bristles 17 are tightly attached to the inner side wall of the tank body 1, and a handle 14 is fixedly connected with the top of the sliding rod 13;
through the research on the fragmentation mechanism of the patulin molecules under the gas phase condition, the spontaneity of the reaction in the thermodynamics of the chemical reaction is disclosed, and the dominant path of the reaction is searched from the chemical kinetics perspective, so that the theoretical cushion is made for the next theoretical or experimental research on the polymerization reaction, the polyaddition reaction, the dissociation or degradation and even the catalytic reaction of the patulin molecules or ions in liquid phase and solution, and a certain theoretical model support is provided for other scientists in researching the detection, the separation and the degradation of the patulin.
In the invention, when the device is used, firstly, the tank body 1 is stably placed on a table top, then the handle 12 is pulled to open the sliding door 11 fixedly connected with the side wall of the handle 12, then the reagent tube 9 is placed on the inner side wall of the placing groove formed in the top of the placing block 8, then the reagent tube 9 is pressed downwards, so that the positioning groove 21 formed in the side wall of the placing block 8 reaches the end part of the positioning block 20, at this time, the positioning block 20 enters the inner side wall of the positioning groove 21 under the action of the first return spring 19 to complete the fixation of the placing block 8, the reagent tube 9 is prevented from turning over when liquid is added into the reagent tube 9, the stability of the reagent tube 9 is improved, then the solution required by the test is added through the first feeding tube 2 and the second feeding tube 3, and due to the addition of the solution, the sealing plate 23 is pressed by the solution, so that the outer side wall of the sealing plate 23 cannot be tightly attached to the side wall of the magnet block 22, after the solution is added, the sealing plate 23 is attached to the side wall of the magnet block 22 again under the action of the torsion spring 25, so that the feeding tube is sealed, and the degradation process is prevented from being influenced by the entering of external bacteria during the degradation of the patulin;
at this moment, through pressing down handle 14, the downward movement of handle 14 can drive the downward slip with slide bar 13 of handle 14 bottom fixed connection, and then drive the extrusion clearance board 15 with slide bar 13 bottom fixed connection and slide downwards, the through-hole 16 that runs through to set up by extrusion clearance board 15 lateral wall realizes the intensive mixing to mixing solution, observe through glass mirror surface 10 whether the degradation is complete, when degrading completely, through opening solenoid valve 6 and make solution flow into the inside of reagent pipe 9 through the inside of flowing back 5 discharge tubes discharge, at this moment upwards take out reagent pipe 9 and observe the interior solution of reagent pipe 9, the taking out of reagent pipe 9 can make second reset spring 26 produce the pushing action to placing block 8, make placing block 8 reset.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (6)
1. The utility model provides a little biodegradable device for patulin, includes jar body (1), its characterized in that, the lateral wall of jar body (1) runs through fixedly connected with first filling tube (2), the lateral wall of jar body (1) runs through fixedly connected with second filling tube (3), the inside wall fixedly connected with one-way mechanism of second filling tube (3), the inside wall fixedly connected with connecting plate (4) of jar body (1), the lateral wall of connecting plate (4) runs through fixedly connected with fluid-discharge tube (5), the inside wall fixedly connected with solenoid valve (6) of fluid-discharge tube (5), the inside wall fixedly connected with fixed plate (7) of jar body (1), fixed plate (7) are located the below of connecting plate (4), the lateral wall of fixed plate (7) runs through sliding connection and places piece (8), the top of placing piece (8) has seted up the standing groove, the inner side wall of the placing groove is tightly attached with a reagent tube (9), the side wall of the placing block (8) is provided with a clamping and fixing mechanism, the bottom of the placing block (8) is fixedly connected with a second return spring (26), the bottom of the second return spring (26) is fixedly connected with the bottom of the inner side wall of the tank body (1), the side wall of the tank body (1) is fixedly connected with a glass mirror surface (10), the side wall of the tank body (1) is connected with a sliding door (11) in a sliding way, the sliding door (11) is positioned between the connecting plate (4) and the fixing plate (7), the outer side wall of the sliding door (11) is fixedly connected with a handle (12), the top of the tank body (1) is connected with a sliding rod (13) in a penetrating and sliding way, the bottom of the sliding rod (13) is fixedly connected with an extrusion cleaning mechanism, and the top of the sliding rod (13) is fixedly connected with a handle (14);
the one-way mechanism comprises a magnet block (22) fixedly connected with the inner side wall of the second feeding pipe (3), the outer side wall of the magnet block (22) is tightly attached with a sealing plate (23), the end part of the sealing plate (23) is fixedly connected with a rotating shaft (24), the inner side wall of the rotating shaft (24) is fixedly connected with a torsion spring (25), the outer side wall of the rotating shaft (24) is rotatably connected with the inner side wall of the second feeding pipe (3), the end part of the torsion spring (25) is fixedly connected with the inner part of the sealing plate (23), the end part of the torsion spring (25) is fixedly connected with the inner part of the second feeding pipe (3), and the outer side wall of the sealing plate (23) is in sealing sliding connection with the inner side wall of the second feeding pipe (3);
extrusion clearance mechanism include with slide bar (13) bottom fixed connection's extrusion clearance board (15), the lateral wall of extrusion clearance board (15) runs through and has seted up through-hole (16), the lateral wall fixedly connected with brush hair (17) of extrusion clearance board (15), the tip of brush hair (17) closely laminates with the inside wall of the jar body (1).
2. The microbial degradation device for patulin according to claim 1, wherein the clamping and fixing mechanism includes a positioning groove (21) formed on an outer side wall of the placement block (8), a positioning block (20) is tightly attached to an inner side wall of the positioning groove (21), a first return spring (19) is fixedly connected to an end of the positioning block (20), a circular groove (18) is fixedly connected to an end of the first return spring (19), the circular groove (18) is formed in the fixing plate (7), and the outer side wall of the positioning block (20) is connected with the side wall of the fixing plate (7) in a sliding manner.
3. The microbial degradation apparatus for patulin of claim 1, the first feeding pipe (2) and the second feeding pipe (3) are symmetrically arranged, the liquid discharge pipes (5) are arranged in an array, the number of the liquid discharge pipes (5) is three, the electromagnetic valves (6) are arranged in an array, the number of the electromagnetic valves (6) is three, the placing blocks (8) are arranged in an array, the number of the placing blocks (8) is three, the reagent tubes (9) are arranged in an array, the number of the reagent tubes (9) is three, the sliding doors (11) are symmetrically arranged, the number of the sliding doors (11) is two, the handles (12) are symmetrically arranged, the number of the handles (12) is two, the second return springs (26) are arranged in an array, and the number of the second return springs (26) is three.
4. The microbial degradation device for patulin according to claim 1, wherein the bottom of the first feeding tube (2) is disposed obliquely, the bottom of the second feeding tube (3) is disposed obliquely, and the bottom of the inner sidewall of the placement tank is disposed in a hemispherical shape.
5. The microbial degradation device for patulin according to claim 2, characterized in that the outer diameter of the sealing plate (23) is smaller than the inner diameter of the second feeding tube (3), and the outer side wall of the sealing plate (23) is matched with the inner side wall of the second feeding tube (3).
6. The microbial degradation device of claim 2, wherein the first return spring (19) is made of brass, and the first return spring (19) is in a compressed state.
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