CN112501243A - Method for verifying sterilization rate of ultraviolet disinfection box - Google Patents
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- CN112501243A CN112501243A CN202011409961.XA CN202011409961A CN112501243A CN 112501243 A CN112501243 A CN 112501243A CN 202011409961 A CN202011409961 A CN 202011409961A CN 112501243 A CN112501243 A CN 112501243A
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- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 105
- 230000001954 sterilising effect Effects 0.000 title claims abstract description 77
- 238000000034 method Methods 0.000 title claims abstract description 22
- 230000001580 bacterial effect Effects 0.000 claims abstract description 58
- 239000000725 suspension Substances 0.000 claims abstract description 42
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 40
- 241000894006 Bacteria Species 0.000 claims abstract description 27
- 238000012360 testing method Methods 0.000 claims abstract description 21
- 239000011780 sodium chloride Substances 0.000 claims abstract description 20
- 238000012795 verification Methods 0.000 claims abstract description 12
- 239000003085 diluting agent Substances 0.000 claims abstract description 9
- 239000013641 positive control Substances 0.000 claims abstract description 9
- 239000003480 eluent Substances 0.000 claims abstract description 6
- 241000191967 Staphylococcus aureus Species 0.000 claims description 12
- 239000003153 chemical reaction reagent Substances 0.000 claims description 12
- 239000001963 growth medium Substances 0.000 claims description 12
- 241000222122 Candida albicans Species 0.000 claims description 10
- 241000588724 Escherichia coli Species 0.000 claims description 10
- 241000233866 Fungi Species 0.000 claims description 10
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims description 10
- 229940095731 candida albicans Drugs 0.000 claims description 10
- 238000002474 experimental method Methods 0.000 claims description 8
- 229920001817 Agar Polymers 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 239000008121 dextrose Substances 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 238000010828 elution Methods 0.000 claims description 5
- 238000009630 liquid culture Methods 0.000 claims description 4
- 239000012137 tryptone Substances 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000007865 diluting Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 238000011084 recovery Methods 0.000 description 3
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical compound CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 108010050327 trypticase-soy broth Proteins 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/22—Testing for sterility conditions
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/21—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Pseudomonadaceae (F)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- G01N2333/245—Escherichia (G)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/305—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F)
- G01N2333/31—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F) from Staphylococcus (G)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/37—Assays involving biological materials from specific organisms or of a specific nature from fungi
- G01N2333/39—Assays involving biological materials from specific organisms or of a specific nature from fungi from yeasts
- G01N2333/40—Assays involving biological materials from specific organisms or of a specific nature from fungi from yeasts from Candida
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- Chemical & Material Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
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- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
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- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
Abstract
The invention discloses a sterilization rate verification method of an ultraviolet sterilization box, which comprises the steps of treating and sterilizing various culture mediums in a high-temperature high-pressure steam furnace for later use, and preparing 0.9% sodium chloride diluent and eluent; then sterilizing the surface of the object to be in an aseptic state; preparing bacterial suspension, sucking the prepared bacterial suspension to a position on the surface of a sterilized object which is most difficult to sterilize, and preparing bacterial tablets; and then, respectively testing the infected objects with the ultraviolet disinfection box test group and the positive control group to obtain the number of bacteria, and finally judging whether the infected objects are qualified according to the sterilization rate, wherein the sterilization rate verification step is carried out so as to achieve the beneficial effect of accurately and efficiently verifying the sterilization rate of the ultraviolet disinfection box.
Description
Technical Field
The invention relates to the technical field of sanitation sterilization, in particular to a method for verifying the sterilization rate of an ultraviolet sterilization box.
Background
Along with the improvement of the consciousness of people for living health prevention, clothes, food, live and walk in life are required to meet the health standard after disinfection and sterilization, for example, people often carry out alcohol disinfection treatment on mobile phones and glasses, and carry out ultraviolet irradiation disinfection treatment on clothes and food, and the like, so as to prevent physical health through disinfection and sterilization measures.
At present, the ultraviolet sterilization lamp is used for irradiation for disinfection and sterilization, is one of the common domestic physical disinfection methods, and is widely applied to scientific research and production departments of medicine, health, biological products and the like for disinfection treatment of air, water and object surfaces. Referring to the iodine-containing disinfectant hygienic standard, the sterilization rate of bacterial propagules is more than 99.999 percent and the sterilization rate of fungi is more than 99.99 percent after ultraviolet sterilization is designed to reach the medical grade sterilization level, so that the verification of the ultraviolet sterilization rate is crucial, and the product sterilization rate is qualified only when the sterilization rate is qualified in the place where the bacteria are most difficult to kill.
Disclosure of Invention
In order to solve the problems, the invention provides a method for verifying the sterilization rate of an ultraviolet disinfection box, which can accurately and efficiently verify the sterilization rate of the ultraviolet disinfection box.
Based on the above, the invention provides a method for verifying the sterilization rate of an ultraviolet disinfection box, which comprises the following steps:
s1: preparing a sterilization culture medium and a reagent: treating and sterilizing a tryptone soytone liquid culture medium, a Sabouraud's dextrose liquid culture medium, a tryptone soytone agar culture medium and a Sabouraud's dextrose agar culture medium in a high-temperature high-pressure steam furnace for later use, and preparing a sodium chloride diluent and an eluent with the concentration of 0.9 percent;
s2: sterilizing the surface of the object; cleaning and drying an object to be sterilized, sterilizing the object at high temperature and high pressure, and detecting the surface of the object to be sterilized to be in an aseptic state;
s3: preparing a bacterial suspension: preparing a bacteria test tube inclined plane by a bacteria passage method, inoculating a fresh culture of scraped bacteria into a sodium chloride test tube filled with 5mL of bacteria suspension with the concentration, roughly measuring the bacteria concentration of the bacteria suspension with a Mach turbidimeter, counting the viable bacteria of the bacteria solution, diluting the bacteria suspension into the bacteria suspension with the bacteria content of 5 multiplied by 105 to 5 multiplied by 106cfu/mL by using sodium chloride diluent with the concentration, and determining the number of bacterial colonies;
s4: preparing a fungus tablet: sucking the bacterial suspension obtained in the dripping step S3 into the position where the surface of the object sterilized in the step S2 is the most difficult to sterilize, placing the object flat in a sterilization plate, and then placing the object in a 37 ℃ thermostat for 30min for drying treatment;
s5: bacterial count experiments: the kit is divided into an ultraviolet disinfection box test group and a positive control group, wherein the ultraviolet disinfection box test group comprises: placing the contaminated object in the step S4 into an ultraviolet disinfection box for disinfection, then eluting the sterilized object with a sodium chloride reagent with the concentration, sucking the eluted object into a culture dish, pouring the eluted object as a flat plate, inoculating two flat plates for culture, and counting the flat plates; the positive control group was: directly placing the infected objects in the step S4 in a sodium chloride reagent with the concentration for elution, sucking the objects into a culture dish for pouring as a flat plate, inoculating two flat plates for culture, and counting the flat plates;
s6: and judging the result, according to a calculation formula of the sterilization rate:
and judging that the sterilization rate reaches the target, and determining that the sterilization rate is qualified.
In the above technical solution, the temperature of the high-temperature high-pressure steam furnace in the step S1 is 121 ℃, and the time is 20 min.
In the above technical solution, the bacterial suspension prepared by the bacterial suspension preparation method in step S3 includes a bacterial suspension of staphylococcus aureus, a bacterial suspension of escherichia coli, a bacterial suspension of pseudomonas aeruginosa, and a bacterial suspension of candida albicans.
In the above technical solution, in the step S4, 0.1mL of bacterial suspension with a bacterial content of 5 × 106cfu/mL is sucked on the surface of the sterilized object, the dripping area is 2 × 3cm, and the bacterial content of the carrier recovery is 5 × 105 cfu/carrier recovery.
In the above technical solution, in the step S4, the surface of the sterilized object is inoculated with bacterial strains of staphylococcus aureus, escherichia coli, pseudomonas aeruginosa and candida albicans respectively.
In the above technical solution, in step S5, the ultraviolet disinfection box test group puts the contaminated object into the ultraviolet disinfection box for disinfection for 60S, and uses 100ml of 0.9% sodium chloride reagent for elution, and sucks 1ml of the contaminated object into the culture dish.
In the above technical solution, in the step S5, the ratio of the bacterial propagule: the culture temperature of the staphylococcus aureus, the escherichia coli and the pseudomonas aeruginosa is 30-37 ℃, and the culture time is 3 days; fungi: the culture temperature of the candida albicans is 20-25 ℃, and the culture time is 5 days.
The invention relates to a sterilization rate verification method of an ultraviolet disinfection box, which comprises the steps of treating and sterilizing various culture mediums in a high-temperature high-pressure steam furnace for later use, and preparing 0.9% sodium chloride diluent and eluent; then sterilizing the surface of the object to be in an aseptic state; preparing bacterial suspension, sucking the prepared bacterial suspension to a position on the surface of a sterilized object which is most difficult to sterilize, and preparing bacterial tablets; and then, respectively testing the infected objects with the ultraviolet disinfection box test group and the positive control group to obtain the number of bacteria, and finally judging whether the infected objects are qualified according to the sterilization rate, wherein the sterilization rate verification step is carried out so as to achieve the beneficial effect of accurately and efficiently verifying the sterilization rate of the ultraviolet disinfection box.
Drawings
FIG. 1 is a flow chart of the method for verifying the sterilization rate of the ultraviolet disinfection box.
Detailed Description
The following detailed description of embodiments of the present invention is provided in connection with the accompanying drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Referring to fig. 1, the method for verifying the sterilization rate of an ultraviolet disinfection box disclosed by the invention comprises the following steps:
s1: preparing a sterilization culture medium and a reagent: in this example, the culture media were trypticase Soy peptone broth, Sabouraud's dextrose broth, trypticase Soy peptone agar medium, and Sabouraud's dextrose agar medium, respectively. Treating and sterilizing in a high-temperature high-pressure steam furnace for later use, wherein the temperature of the high-temperature high-pressure steam furnace is set to 121 ℃, and the action time is 20 min;
preparing 0.9% sodium chloride diluent and eluent reagent;
s2: and (3) sterilizing the surface of the object: cleaning and drying an object to be sterilized, sterilizing the object at high temperature and high pressure, and detecting the surface of the object to be sterilized to be in an aseptic state;
s3: preparing a bacterial suspension: taking staphylococcus aureus as an example, preparing a test tube inclined plane of the staphylococcus aureus by a strain passage method, scraping a fresh culture of the staphylococcus aureus by an inoculating loop into a sodium chloride test tube filled with 5mL of 0.9 percent, preliminarily preparing a bacterial suspension, roughly measuring the bacterial concentration of the bacterial suspension with a Mach's turbidimeter, counting the viable bacteria of the bacterial suspension, diluting the bacterial suspension into a bacterial suspension with the bacterial content of 5 multiplied by 105 to 5 multiplied by 106cfu/mL by using a sodium chloride diluent with the concentration, and determining the number of bacterial colonies.
In addition, bacterial suspensions of Escherichia coli, Pseudomonas aeruginosa, Candida albicans can be prepared by the same methods as described above.
S4: preparing a fungus tablet: sucking 0.1mL of bacterial suspension of 5 × 106cfu/mL, dripping 2 × 3cm of the bacterial suspension on the surface of a sterilized object at the position which is most difficult to sterilize, placing the bacterial suspension in a sterilization plate horizontally, placing the sterilization plate and the sterilization plate together in a 37 ℃ thermostat for 30min for drying, dripping a certain amount of bacterial liquid into each carrier, and specifically, the carrier recovery bacterial quantity reaches 5 × 105 cfu/carrier.
Wherein, the inoculum type is respectively bacterial propagule and fungi, and the bacterial propagule comprises: staphylococcus aureus, escherichia coli, pseudomonas aeruginosa; the fungus is Candida albicans.
S5: bacterial count experiments: the experiment was divided into an ultraviolet disinfection box test group and a positive control group, wherein,
the ultraviolet disinfection box test group is as follows: and (4) sterilizing the infected objects in the step S4 in an ultraviolet sterilization box for 60S, then eluting with 100ml of 0.9% sodium chloride reagent, sucking 1ml of the solution into a culture dish, pouring the solution as a plate, inoculating two plates for culture, and counting the plates. Wherein the culture temperature of the staphylococcus aureus, the escherichia coli and the pseudomonas aeruginosa is 30-37 ℃ for 3 days; fungi: the culture temperature of the candida albicans is 20-25 ℃ and the culture time is 5 days.
The positive control group was: directly placing the infected objects in 100ml of 0.9% sodium chloride reagent for elution, sucking 1ml of the eluted objects into a culture dish, pouring the eluted objects as a flat plate, inoculating two flat plates for culture, and counting the number of the flat plates. Similarly, the culture temperature of the staphylococcus aureus, the escherichia coli and the pseudomonas aeruginosa is 30-37 ℃ for 3 days; fungi: the culture temperature of the candida albicans is 20-25 ℃ and the culture time is 5 days.
And performing three parallel experiments in the same group of experiments, testing the results of the three groups of experiments, and taking the lowest sterilization rate value in the three groups as the sterilization result.
S6: and judging the result, according to a calculation formula of the sterilization rate:
and the sterilization rates of the three parallel experiments all reach the target and are judged to be qualified.
In summary, the sterilization rate verification method for the ultraviolet disinfection box of the invention comprises the steps of treating and sterilizing various culture mediums in a high-temperature high-pressure steam furnace for standby, and preparing 0.9% sodium chloride diluent and eluent; then sterilizing the surface of the object to be in an aseptic state; preparing bacterial suspension, sucking the prepared bacterial suspension to a position on the surface of a sterilized object which is most difficult to sterilize, and preparing bacterial tablets; and then, respectively testing the infected objects with the ultraviolet disinfection box test group and the positive control group to obtain the number of bacteria, and finally judging whether the infected objects are qualified according to the sterilization rate, wherein the sterilization rate verification step is carried out so as to achieve the beneficial effect of accurately and efficiently verifying the sterilization rate of the ultraviolet disinfection box.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and substitutions can be made without departing from the technical principle of the present invention, and these modifications and substitutions should also be regarded as the protection scope of the present invention.
Claims (7)
1. A sterilization rate verification method for an ultraviolet disinfection box is characterized by comprising the following steps:
s1: preparing a sterilization culture medium and a reagent: treating and sterilizing a tryptone soytone liquid culture medium, a Sabouraud's dextrose liquid culture medium, a tryptone soytone agar culture medium and a Sabouraud's dextrose agar culture medium in a high-temperature high-pressure steam furnace for later use, and preparing a sodium chloride diluent and an eluent with the concentration of 0.9 percent;
s2: sterilizing the surface of the object; cleaning and drying an object to be sterilized, sterilizing the object at high temperature and high pressure, and detecting the surface of the object to be sterilized to be in an aseptic state;
s3: preparing a bacterial suspension: preparing a bacteria test tube inclined plane by a bacteria passage method, inoculating a fresh culture of scraped bacteria into a sodium chloride test tube filled with 5mL of bacteria suspension with the concentration, roughly measuring the bacteria concentration of the bacteria suspension with a Mach turbidimeter, counting the viable bacteria of the bacteria solution, diluting the bacteria suspension into the bacteria suspension with the bacteria content of 5 multiplied by 105 to 5 multiplied by 106cfu/mL by using sodium chloride diluent with the concentration, and determining the number of bacterial colonies;
s4: preparing a fungus tablet: sucking the bacterial suspension obtained in the dripping step S3 into the position where the surface of the object sterilized in the step S2 is the most difficult to sterilize, placing the object flat in a sterilization plate, and then placing the object in a 37 ℃ thermostat for 30min for drying treatment;
s5: bacterial count experiments: is divided into an ultraviolet disinfection box test group and a positive control group, wherein,
the ultraviolet disinfection box test group is as follows: placing the contaminated object in the step S4 into an ultraviolet disinfection box for disinfection, then eluting the sterilized object with a sodium chloride reagent with the concentration, sucking the eluted object into a culture dish, pouring the eluted object as a flat plate, inoculating two flat plates for culture, and counting the flat plates;
the positive control group was: directly placing the infected objects in the step S4 in a sodium chloride reagent with the concentration for elution, sucking the objects into a culture dish for pouring as a flat plate, inoculating two flat plates for culture, and counting the flat plates;
s6: and judging the result, according to a calculation formula of the sterilization rate:
and judging that the sterilization rate reaches the target, and determining that the sterilization rate is qualified.
2. The ultraviolet sterilization box sterilization rate verification method of claim 1, wherein the temperature of the high-temperature high-pressure steam oven in the step S1 is 121 ℃ and the time is 20 min.
3. The method for verifying the sterilization rate of an ultraviolet disinfection box as claimed in claim 1, wherein the bacterial suspension prepared by the bacterial suspension preparation method in step S3 comprises a bacterial suspension of staphylococcus aureus, a bacterial suspension of escherichia coli, a bacterial suspension of pseudomonas aeruginosa and a bacterial suspension of candida albicans.
4. The method for verifying the sterilization rate of an ultraviolet disinfection box according to claim 3, wherein 0.1mL of bacterial suspension with the bacterial content of 5 x 106cfu/mL is sucked on the surface of the sterilized object in step S4, the dripping area is 2 x 3cm, and the bacterial content of the recovered carrier is 5 x 105 cfu/piece.
5. The ultraviolet disinfection box sterilization rate verification method of claim 4, wherein in step S4, the surface of the sterilized object is inoculated with bacterial strains of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans.
6. The ultraviolet disinfection box sterilization rate verification method of claim 5, wherein in step S5, the ultraviolet disinfection box test group puts the infected objects into the ultraviolet disinfection box for disinfection for 60S, and uses 100ml of 0.9% sodium chloride reagent for elution, and sucks 1ml into the culture dish.
7. The ultraviolet disinfection box sterilization rate verification method of claim 6, wherein in step S5, the ratio of the bacterial propagules: the culture temperature of the staphylococcus aureus, the escherichia coli and the pseudomonas aeruginosa is 30-37 ℃, and the culture time is 3 days; fungi: the culture temperature of the candida albicans is 20-25 ℃, and the culture time is 5 days.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116617434A (en) * | 2023-05-12 | 2023-08-22 | 苏州苏大卫生与环境技术研究所有限公司 | Automatic disinfection effect verification system capable of reusing medical equipment |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1205898A (en) * | 1998-08-19 | 1999-01-27 | 卢杲 | Household ultraviolet disinfector |
| US20010048891A1 (en) * | 2000-02-01 | 2001-12-06 | Mcgeorge Gram J. | Method and apparatus for verifying ultraviolent sterilization |
| US20140356229A1 (en) * | 2010-06-01 | 2014-12-04 | Bluemorph Llc | Uv devices, systems and methods for uv sterilization |
| CN104707158A (en) * | 2013-12-12 | 2015-06-17 | 罗旭 | Method and device for detecting ultraviolet (UV) sterilization effect |
-
2020
- 2020-12-05 CN CN202011409961.XA patent/CN112501243A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1205898A (en) * | 1998-08-19 | 1999-01-27 | 卢杲 | Household ultraviolet disinfector |
| US20010048891A1 (en) * | 2000-02-01 | 2001-12-06 | Mcgeorge Gram J. | Method and apparatus for verifying ultraviolent sterilization |
| US20140356229A1 (en) * | 2010-06-01 | 2014-12-04 | Bluemorph Llc | Uv devices, systems and methods for uv sterilization |
| CN104707158A (en) * | 2013-12-12 | 2015-06-17 | 罗旭 | Method and device for detecting ultraviolet (UV) sterilization effect |
Non-Patent Citations (4)
| Title |
|---|
| 中华人民共和国卫生部: "《消毒技术规范(2002年版)》", 30 November 2002, 中华人民共和国国家卫生健康委员会HTTP://WWW.NHC.GOV.CN/WJW/GFXWJ/201304/3A0121CBA422455B93307F070B099CF2.SHTML * |
| 居喜娟等: "紫外线消毒箱消毒效果的研究", 《中国消毒学杂志》 * |
| 顾健、周士新: "CXW-170电子消毒柜对微生物杀灭效果的研究", 《中华医院感染学杂志》 * |
| 龙建友、阎佳: "《环境工程微生物实验教程》", 31 January 2019, 北京理工大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116617434A (en) * | 2023-05-12 | 2023-08-22 | 苏州苏大卫生与环境技术研究所有限公司 | Automatic disinfection effect verification system capable of reusing medical equipment |
| CN116617434B (en) * | 2023-05-12 | 2024-08-30 | 苏州苏大卫生与环境技术研究所有限公司 | Automatic disinfection effect verification system capable of reusing medical equipment |
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