CN104707158A - Method and device for detecting ultraviolet (UV) sterilization effect - Google Patents
Method and device for detecting ultraviolet (UV) sterilization effect Download PDFInfo
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- Apparatus For Disinfection Or Sterilisation (AREA)
Abstract
The invention discloses a method and device for detecting ultraviolet (UV) sterilization effect. The method comprises the following steps: (1) determining the relationship among the UV radiation strength, UV radiation time, and sterilization rate; (2) detecting the UV radiation strength; (3) obtaining the sterilization rate according to the UV radiation strength and a given radiation time. The device comprises a UV strength detection device, a time setting device, and a sterilization rate calculation module. The method and device can guarantee the effectiveness of a UV sterilization facility on killing bacteria (especially staphylococcus aureus), the sterilization process, which cannot be observed by naked eyes, can be evaluated and controlled, moreover, the UV sterilization facility can be stopped after the staphylococcus aureus is completely killed, the energy waste is avoided, and the practicality of the UV sterilization facility is improved.
Description
Technical field
Patent of the present invention relates to a kind of bactericidal effect detection method, is specifically related to a kind of method and the device that detect ultraviolet kill bacteria effect.
Background technology
As everyone knows, ultraviolet has sterilizing function, such as utilize the function of the UV-C wavelength (200-280nm) in ultraviolet (wave-length coverage 380nm-200nm) to the radiation damage of microorganism and destruction nucleic acid to make microbial death, thus reach bactericidal effect.In the modern life, occur utilizing ultraviolet to carry out the product such as sterilamp, disinfection cabinet of sterilizing.But, the ultraviolet lamp tube of these products has service life, along with the prolongation of service time, fluorescent tube is aging gradually, light intensity will lose 30%-50%, when ultraviolet ray intensity does not reach the national disinfection standard of 300J/cm2, there is photoreactivation phenomenon in microbes, so just cannot complete the effect of thorough disinfection.Therefore the bactericidal effect of clear and definite disinfection by ultraviolet light product is needed.
For staphylococcus aureus, this coccus is a kind of important pathogen of the mankind, extensively be present among various medium, because staphylococcus aureus can produce plasma-coagulase, staphylolysin, leukocidin, enterotoxin, epidermolytic toxin, Toxic Shock Syndrome Toxin I (Toxic shock syndrome toxinl, and Sbphylokinase TTST1), the various toxin such as hyaluronidase, adult and the various infectious disease of infant can be caused, common disease has toxic intestinal scorching, exfoliative dermatitis etc., serious caused general toxicity shock, many histoorgans are downright bad, severe skin scalds sample syndrome (Staphylococcal Scalded Skin Syndrome, be called for short SSSS) etc. fatal disease.According to FDA (Food and Drug Adminstration) (FDA) in May, 2009, in Texas, USA 16 kindergartens alimentary toxicosis case that totally 5824 routine infants occur, 1364 examples are had to be that staphylococcus aureus causes, wherein 8 examples die from the intestinal infection of this bacterium food source property, finally do not control with serious symptom general toxicity shock.In the sanitary standard of all departments, the quantity of staphylococcus aureus is all one of major criterion.And the common mode of killing of staphylococcus aureus is exactly disinfection by ultraviolet light, the bactericidal effect of the various sterile products of existing detection, especially the effect of staphylococcus aureus is killed in real time, also do not utilize sensor light method for electrically to realize, the disinfection apparatus such as sterilamp, disinfection cabinet also never clearly indicates the degree appeared the various antibacterials such as staphylococcus aureus and be killed, and disinfection therefore may be caused to enter into mistaken ideas.Because the method detecting Disinfection Effect is accurate not, therefore create the mistaken ideas that a lot of disinfection by ultraviolet light facility uses, after as aging in ultraviolet disinfection lamp, still carry out disinfection according to normal sterilisation step, thus cause extent of killing bacteria not enough, cause the continuation of pathogen to propagate.
Summary of the invention
The object of the invention is to provide a kind of by utilizing electronic machine technology for detection uitraviolet intensity, the method and apparatus of killing bacteria effect is judged according to uitraviolet intensity, accurately detect the percentage ratio that antibacterial is killed under ultraviolet radiation, the pathogen caused because killing bacteria degree is not enough is avoided to continue to propagate, to ensure that ultraviolet disinfection equipment reaches the disinfective action of standard.
For achieving the above object, technical solution of the present invention is as follows: a kind of method detecting ultraviolet kill bacteria effect, comprises the steps:
First, ultraviolet irradiation intensity (P), relation between ultraviolet irradiation time (t) and bacterial inactivation rate (Q) is obtained;
Q=K*P*t, wherein K is for the antibacterial determining kind, is constant, and the unit of t is minute, and P unit is microwatt/square centimeter, and the unit of K is the unit of square centimeter/(microwatt * minute), Q is %.
For staphylococcus aureus, K=0.538.
For hay bacterium, K=0.277.
For escherichia coli, K=0.634.
Secondly, detect the ultraviolet irradiation intensity of ultraviolet disinfection instrument, setting ultraviolet irradiation time (t);
3rd, according to the irradiation time (t) of ultraviolet irradiation intensity (P) and setting, obtain the killing rate (Q) of antibacterial.
Described ultraviolet irradiation intensity (P), relation between ultraviolet irradiation time (t) and bacterial inactivation rate (Q) obtain with statistical method by experiment.
Described antibacterial is staphylococcus aureus.
Described ultraviolet irradiation intensity, pass between ultraviolet irradiation time and staphylococcus aureus killing rate are: staphylococcus aureus Continuous irradiation 3 minutes under the ultraviolet irradiation intensity of 90 microwatts/square centimeter or more, realizes the sterilizing rate of 100%; Under the ultraviolet irradiation intensity of 20 microwatts/square centimeter, Continuous irradiation 3 minutes, realizes the sterilizing rate of 32%.
Adopt uitraviolet intensity checkout gear to detect ultraviolet irradiation intensity, uitraviolet intensity checkout gear comprises UV photodetector, differential amplifier circuit and testing circuit,
UV photodetector connects differential amplifier circuit, differential amplifier circuit connection detection circuit, UV photodetector receives ultraviolet light and ultraviolet light is converted to the signal of telecommunication and is sent to differential amplifier circuit and carries out processing and amplifying, detects after processing and amplifying in signal input testing circuit.
Described UV photodetector adopts GaN/AlGaN heterojunction photodiode to receive ultraviolet.
The value of electrical signals that differential amplifier circuit exports by the described testing circuit value of electrical signals corresponding with national standard irradiation under ultraviolet ray intensity compares, and judges the irradiation under ultraviolet ray intensity whether conformance with standard of ultraviolet disinfection instrument.
Described uitraviolet intensity checkout gear comprises a shell, and UV photodetector is arranged on shell, and differential amplifier circuit and testing circuit are arranged in the enclosure.
Described uitraviolet intensity checkout gear comprises a location supporting rod, for locating the distance between ultraviolet disinfection instrument and UV photodetector.
Described location supporting rod is arranged on shell.
Described differential amplifier circuit and testing circuit become one.
Described shell arranges display lamp, and testing circuit connects and controls display lamp.
The present invention also provides a kind of device detecting ultraviolet kill bacteria effect, comprise uitraviolet intensity checkout gear, time-setting mechanism and sterilizing rate computing module, uitraviolet intensity checkout gear detects the uitraviolet intensity of ultraviolet disinfection instrument and uitraviolet intensity data is inputted sterilizing rate computing module, sterilizing rate computing module is according to ultraviolet irradiation intensity, relation between ultraviolet irradiation time and bacterial inactivation rate calculates sterilizing rate, time-setting mechanism is by set time numerical value input sterilizing rate computing module, uitraviolet intensity checkout gear comprises UV photodetector, differential amplifier circuit and testing circuit, UV photodetector connects differential amplifier circuit, differential amplifier circuit connection detection circuit, UV photodetector receives ultraviolet light and ultraviolet light is converted to the signal of telecommunication and is sent to differential amplifier circuit and carries out processing and amplifying, detect in signal input testing circuit after processing and amplifying, ultraviolet irradiation intensity, pass between ultraviolet irradiation time and bacterial inactivation rate are: Q=K*P*t, wherein P is ultraviolet irradiation intensity, and t is ultraviolet irradiation time, and Q is bacterial inactivation rate, K is that specific bacteria ultraviolet kills coefficient, is constant to specific bacteria.
The device of described detection ultraviolet kill bacteria effect also comprises display device, and display device is connected with sterilizing rate computing module, display sterilizing rate.
Described uitraviolet intensity checkout gear comprises a location supporting rod, for locating the distance between ultraviolet disinfection instrument and UV photodetector.
Beneficial effect of the present invention is:
The present invention can ensure UV sterilizer kill bacteria, especially the effectiveness of staphylococcus aureus, make originally the sterilization process of observation effect cannot have evaluation criterion and control device, staphylococcus aureus can also be avoided all to kill rear continuation consumed energy, increase the practicality of UV sterilizer.
Further, the present invention also can avoid in order to pursue 100% kill staphylococcus aureus time, excessively use ultraviolet light source and the energy waste that causes and extras loss.
Ultraviolet detecting apparatus of the present invention utilizes ultraviolet detector to detect ultraviolet disinfection instrument, can real-time detection ultraviolet disinfection intensity, and work in room temperature condition, simple to operate, volume is little, easy for installation, long service life, thus make sterilization more stable, thorough, reach state health standards.
In recent years, the progress of iii-v nitride wide bandgap semiconductor (Eg>2.3eV) is rapidly abnormal.Be wherein forward position and the focus that the preparation of GaN base semiconductor material with wide forbidden band of representative and the development of device have become the research of current global semiconductor with GaN.GaN base material belongs to direct energy-gap semiconductor material, energy gap is from 3.4eV-6.2eV continuously adjustabe, radiation recombination is high, electric breakdown strength high (~ 3 × 106V/cm), leakage current is little, electron drift saturated velocity is high, good heat conductivity, chemistry and physical property is stable and radiation resistance is high, these various devices prepared by GaN base material can be applicable to high-power, high frequency, the adverse circumstances such as high temperature, and all achieve outstanding achievement in scientific research and application, thus make GaN base material become the first generation Ge that continues, Si element semiconductor material, second filial generation GaAs, InP, third generation semi-conducting material after the compound semiconductor materials such as InAs.
The energy gap that GaN is suitable and Direct-type band structure, this new material is made extremely to be suitable for developing the photoelectric device of visible region short-wave band and ultraviolet band, such as blue light-emitting diode (LED) and UV photodetector etc., further, the development of UV photodetector reaches realistic scale.UV photodetector is the photodetector utilizing the work of GaN/AlGaN heterojunction photodiode, and its stable performance, easy to use, the life-span is long, has started to be applied to national defence, scientific research, the various field such as civilian.
Accompanying drawing explanation
Fig. 1 is the structural representation of uitraviolet intensity checkout gear of the present invention.
Fig. 2 is the circuit theory diagrams of uitraviolet intensity checkout gear of the present invention.
Fig. 3 is the structural representation of the device of detection ultraviolet kill bacteria effect of the present invention.
Detailed description of the invention
First the killing test of staphylococcus aureus is carried out, use four culture dishs, upper inoculation has staphylococcus aureus, white point is bacterium colony, result after ultraviolet radiation is as follows, and A culture dish is the Continuous irradiation killing effect of 3 minutes under the ultraviolet irradiation intensity of 90 microwatts/square centimeter, and sterilizing rate is about 100%, B culture dish is the Continuous irradiation killing effect of 3 minutes under the ultraviolet irradiation intensity of 40 microwatts/square centimeter, and sterilizing rate is about 65%; C culture dish is the Continuous irradiation killing effect of 3 minutes under the ultraviolet irradiation intensity of 20 microwatts/square centimeter, and sterilizing rate is about 32%; D culture dish is the Continuous irradiation killing effect of 3 minutes under the ultraviolet irradiation intensity of 10 microwatts/square centimeter.Also carried out the test of other exposure rate in addition, staphylococcus aureus respectively 80, Continuous irradiation 4 minutes under the ultraviolet irradiation intensity of 100 microwatts/square centimeter, after inspection, result is the sterilizing rate of 100%.Continuous irradiation 3 minutes under the ultraviolet irradiation intensity of 0 microwatt/square centimeter, after inspection, result is the sterilizing rate of 0%.According to above-mentioned test, the invention discloses following method:
A kind of method detecting ultraviolet kill bacteria effect, to kill staphylococcus aureus, its step comprises: tested the relation determined ultraviolet irradiation intensity, irradiation time and kill between staphylococcus aureus ratio by staphylococcus aureus killing effect; This relation obtains with statistical method by experiment, constantly gathers uitraviolet intensity numerical value and ultraviolet irradiation time numerical value, and the corresponding killing rate of staphylococcus aureus, obtains this relation by statistical method.Q=K*P*t, wherein P is ultraviolet irradiation intensity, and t is ultraviolet irradiation time, and Q is bacterial inactivation rate, and K is that specific bacteria ultraviolet kills coefficient, is constant to specific bacteria.For hay bacterium, K=0.277; For escherichia coli, K=0.634; For staphylococcus aureus, K=0.538.
Second step, detects the ultraviolet irradiation intensity of ultraviolet disinfection instrument, setting ultraviolet irradiation time (t); Description below concrete testing process is visible.
3rd step, according to the irradiation time of ultraviolet irradiation intensity and setting, obtains the killing rate of antibacterial.This killing rate can show.
Uitraviolet intensity checkout gear is adopted to detect in real time ultraviolet disinfection instrument ultraviolet light intensity in embodiment.
As shown in Figure 1, the checkout gear of ultraviolet disinfection instrument ultraviolet light intensity comprises location supporting rod 2, UV photodetector 3, differential amplifier circuit 4 and testing circuit 5.Checkout gear utilizes UV photodetector 3 that the ultraviolet light that ultraviolet disinfection instrument 1 sends is converted to the signal of telecommunication, and with display lamp display, thus the disinfecting power of ultraviolet disinfection instrument is judged.
Checkout gear medium ultraviolet photodetector 3, differential amplifier circuit 4 and testing circuit 5 are integrated in a standalone module, and its circuit theory diagrams as shown in Figure 2, and are positioned specified distance by location supporting rod 2.The ultraviolet lighting that ultraviolet disinfection instrument 1 sends is mapped on the receive window of the UV photodetector 3 in the detecting module of accurate positioning, and the signal of telecommunication of output utilizes differential amplifier circuit 4 to carry out processing and amplifying, finally utilizes testing circuit 5 to carry out detection and exports.
Checkout gear comprises a shell 8, and UV photodetector 3 is arranged on shell 8, and differential amplifier circuit 4 and testing circuit 5 are arranged in shell 8.Location supporting rod 2 is also arranged on shell 8, for the distance between position detecting system ultraviolet disinfection instrument 1 and UV photodetector 3.
Shell 8 is provided with two display lamps, if ultraviolet ray intensity meets the demands, then green light 6 shows, if ultraviolet ray intensity is lower than standard-required, then red light 7 shows, and instruction needs to change ultraviolet disinfecting tube.Two display lamps are connected with testing circuit 5.
UV photodetector 3 is prepared from by GaN/AlGaN heterojunction photodiode, it and differential amplifier circuit 4, testing circuit 5 are integrated in detecting module, located by location supporting rod 2 and formulate distance, and receive the ultraviolet light of the specific wavelength that light source 1 exports, change ultraviolet signal into the signal of telecommunication, and send in differential amplifier circuit 4.Differential amplifier circuit 4 receives the signal of telecommunication that UV photodetector 3 exports, and carries out difference processing amplification, and sends in testing circuit.Testing circuit 5 compares the value of electrical signals value of electrical signals corresponding with national standard ultraviolet ray intensity that differential amplifier circuit 4 exports, if be more than or equal to standard value, then output signal display green light 6 is bright, if be less than standard it, then to show red light 7 bright for output signal.The ultraviolet lamp tube output wavelength that ultraviolet disinfection instrument 1 is different and intensity difference, but the output wavelength of disinfecting tube is all at the UV-C wave band of 200-280nm, wherein best with the disinfection by ultraviolet light ability of about 254nm.
A kind of device detecting ultraviolet kill bacteria effect, as shown in Figure 3, comprise uitraviolet intensity checkout gear 9, time-setting mechanism 10, sterilizing rate computing module 11 and display device 12, sterilizing rate computing module 11 is according to ultraviolet irradiation intensity, relation between ultraviolet irradiation time and bacterial inactivation rate calculates sterilizing rate, uitraviolet intensity checkout gear 9 detects the uitraviolet intensity of ultraviolet disinfection instrument and uitraviolet intensity data is inputted sterilizing rate computing module 11, time-setting mechanism 10 is by set time numerical value input sterilizing rate computing module 11, the concrete structure of uitraviolet intensity detection system 9 is as foregoing description, do not repeat them here.Display device 12 is connected with sterilizing rate computing module 11, and display is through calculating the sterilizing rate obtained.
Detect by the effect killing staphylococcus aureus of above-mentioned system to a medical ultraviolet disinfection instrument, after start, uitraviolet intensity checkout gear 9 detects that the uitraviolet intensity of medical ultraviolet disinfection instrument is 20 microwatts/square centimeter, inputting setting by time-setting mechanism 10, to kill the time be 3 minutes, then system display killing effect is 32%.Adopt this medical ultraviolet disinfection instrument to carry out sterilization to the staphylococcus aureus on culture dish through reality and detect staphylococcus aureus bacterium colony on culture dish after 3 minutes, germicidal efficiency is approximately 32%.Uitraviolet intensity checkout gear 9 detects that the uitraviolet intensity of medical ultraviolet disinfection instrument is 90 microwatts/square centimeter, and inputting setting by time-setting mechanism 10, to kill the time be 3 minutes, then system display killing effect is 145%.Adopt this medical ultraviolet disinfection instrument to carry out sterilization to the staphylococcus aureus on culture dish through reality and detect staphylococcus aureus bacterium colony on culture dish after 3 minutes, germicidal efficiency is 100%.
Claims (10)
1. detect a method for ultraviolet kill bacteria effect, comprise the steps
First, ultraviolet irradiation intensity (P), relation between ultraviolet irradiation time (t) and bacterial inactivation rate (Q) is obtained;
Q=K*P*t, wherein K is for the antibacterial determining kind, is constant;
Secondly, detect the ultraviolet irradiation intensity of ultraviolet disinfection instrument, setting ultraviolet irradiation time (t);
3rd, according to the irradiation time (t) of ultraviolet irradiation intensity (P) and setting, obtain the killing rate (Q) of antibacterial.
2. the as claimed in claim 1 method detecting ultraviolet kill bacteria effect, is characterized in that, described ultraviolet irradiation intensity, relation between ultraviolet irradiation time and bacterial inactivation rate obtain with statistical method by experiment.
3. the method detecting ultraviolet kill bacteria effect as claimed in claim 1, is characterized in that, when antibacterial is staphylococcus aureus, and K=0.538.
4. the method detecting ultraviolet kill bacteria effect as claimed in claim 1, is characterized in that, when described antibacterial is hay bacterium, and K=0.277.
5. the method detecting ultraviolet kill bacteria effect as claimed in claim 1, is characterized in that, when antibacterial is escherichia coli, and K=0.634.
6. the method detecting ultraviolet kill bacteria effect as claimed in claim 1, it is characterized in that, uitraviolet intensity checkout gear is adopted to detect ultraviolet irradiation intensity, uitraviolet intensity checkout gear comprises UV photodetector, differential amplifier circuit and testing circuit, UV photodetector connects differential amplifier circuit, differential amplifier circuit connection detection circuit, UV photodetector receives ultraviolet light and ultraviolet light is converted to the signal of telecommunication and is sent to differential amplifier circuit and carries out processing and amplifying, detects after processing and amplifying in signal input testing circuit.
7. the method detecting ultraviolet kill bacteria effect as claimed in claim 6, is characterized in that, described UV photodetector adopts GaN/AlGaN heterojunction photodiode to receive ultraviolet.
8. one kind is detected the device of ultraviolet kill bacteria effect, it is characterized in that, comprise uitraviolet intensity checkout gear, time-setting mechanism and sterilizing rate computing module, uitraviolet intensity checkout gear detects the uitraviolet intensity of ultraviolet disinfection instrument and uitraviolet intensity data is inputted sterilizing rate computing module, time-setting mechanism is by set time numerical value input sterilizing rate computing module, and sterilizing rate computing module calculates sterilizing rate according to ultraviolet irradiation intensity, relation between ultraviolet irradiation time and bacterial inactivation rate; Uitraviolet intensity checkout gear comprises UV photodetector, differential amplifier circuit and testing circuit, UV photodetector connects differential amplifier circuit, differential amplifier circuit connection detection circuit, UV photodetector receives ultraviolet light and ultraviolet light is converted to the signal of telecommunication and is sent to differential amplifier circuit and carries out processing and amplifying, detects after processing and amplifying in signal input testing circuit.
9. the device detecting ultraviolet kill bacteria effect as claimed in claim 8, it is characterized in that, the value of electrical signals that differential amplifier circuit exports by the described testing circuit value of electrical signals corresponding with national standard ultraviolet ray intensity compares, and judges the ultraviolet ray intensity whether conformance with standard of medical ultraviolet disinfection instrument.
10. the device detecting ultraviolet kill bacteria effect as claimed in claim 8, it is characterized in that, described uitraviolet intensity checkout gear comprises a location supporting rod, for the distance between the medical ultraviolet disinfection instrument of detection and localization and UV photodetector.
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| CN108815544A (en) * | 2018-08-01 | 2018-11-16 | 青岛海尔洗衣机有限公司 | Ultraviolet-sterilization control method and system |
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| CN113491778A (en) * | 2020-03-20 | 2021-10-12 | 同方威视技术股份有限公司 | Disinfection apparatus and disinfection system |
| JP2022001095A (en) * | 2020-06-19 | 2022-01-06 | 株式会社ファームロイド | Ultraviolet irradiation sterilization device, ultraviolet irradiation sterilization system and ultraviolet irradiation sterilization method |
| CN114832138A (en) * | 2022-07-04 | 2022-08-02 | 至善时代智能科技(北京)有限公司 | Method and device for detecting sterilizing effect of UVC ultraviolet radiation |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109890426A (en) * | 2016-10-24 | 2019-06-14 | 锋翔科技公司 | The system and method for bioinactivation |
| CN110121367A (en) * | 2017-01-05 | 2019-08-13 | 标致雪铁龙汽车股份有限公司 | For measuring the method and system of the validity of the chlorination equipment of vehicle cab |
| CN108815544A (en) * | 2018-08-01 | 2018-11-16 | 青岛海尔洗衣机有限公司 | Ultraviolet-sterilization control method and system |
| CN109620983A (en) * | 2019-01-23 | 2019-04-16 | 兰明贤 | A kind of shoes processing ultraviolet irradiation method |
| CN113491778A (en) * | 2020-03-20 | 2021-10-12 | 同方威视技术股份有限公司 | Disinfection apparatus and disinfection system |
| JP2022001095A (en) * | 2020-06-19 | 2022-01-06 | 株式会社ファームロイド | Ultraviolet irradiation sterilization device, ultraviolet irradiation sterilization system and ultraviolet irradiation sterilization method |
| CN112501243A (en) * | 2020-12-05 | 2021-03-16 | 湖南菁益医疗科技有限公司 | Method for verifying sterilization rate of ultraviolet disinfection box |
| CN114832138A (en) * | 2022-07-04 | 2022-08-02 | 至善时代智能科技(北京)有限公司 | Method and device for detecting sterilizing effect of UVC ultraviolet radiation |
| CN114832138B (en) * | 2022-07-04 | 2022-09-30 | 至善时代智能科技(北京)有限公司 | Method and device for detecting sterilizing effect of UVC ultraviolet radiation |
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