CN112501018A

CN112501018A - Cell culture device for medical examination

Info

Publication number: CN112501018A
Application number: CN202011306686.9A
Authority: CN
Inventors: 孙敏
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-11-19
Filing date: 2020-11-19
Publication date: 2021-03-16
Anticipated expiration: 2040-11-19
Also published as: CN112501018B

Abstract

The invention discloses a cell culture device for medical examination, which comprises: a box body; the four supporting legs are fixedly arranged at the four corners of the bottom end of the box body respectively; the transparent plate is arranged in the middle of the front side of the box body and can be opened and closed along the vertical direction; the temperature controller is fixedly arranged at the top end of the front side of the box body; the sealing mechanism is fixedly arranged at the front side position of the bottom end of the transparent plate; the base is fixedly arranged at the bottom end of the inner cavity of the box body; and every two support rods are a group and are fixedly arranged at the left end and the right end of the front side and the rear side of the base respectively along the vertical direction. This cell culture device for medical examination constructs comparatively perfectly, and is higher to space utilization, and the export of taking is less, reduces area of contact and time that contact with external environment, has further guaranteed cell culture's environment, is favorable to the cultivation to the cell.

Description

Cell culture device for medical examination

Technical Field

The invention relates to the technical field of cell culture, in particular to a cell culture device for medical examination.

Background

Pluripotent stem cells (embryonic stem cells, artificial pluripotent stem cells) are considered as an important cell source for regenerative medicine because of their unlimited proliferation potential and multi-differentiation potential. Examples of the regenerative medicine using stem cells include treatment of liver cirrhosis, blood diseases, and myocardial infarction, construction of blood vessels, regeneration of bones and corneas, and securing of skin for transplantation. In regenerative medicine, target cells or internal organs are proliferated in a culture dish using stem cells or the like, and transplanted into a human. Recently, bone marrow-derived stem cells have been used for angiogenesis and successful treatment of angina pectoris, myocardial infarction, and the like;

present cell culture device structure is comparatively single, space utilization in to culture apparatus is not high, when the staff need take to the cell culture dish, need open whole cabinet door, outside bacterium gets into easily this moment, influence the culture environment of inside cell, secondly because the culture dish quantity in the cell culture cabinet is more, still need select it after the staff opens the cabinet door, inside culture cell and external exposure time have been increased, the leakproofness after the culture apparatus cabinet door is opened and shut simultaneously is difficult to guarantee, the above circumstances all does not benefit to the cultivation of cell, therefore we propose a cell culture device for medical examination.

Disclosure of Invention

The invention aims to provide a cell culture device for medical examination, which at least solves the problems that the opening and closing area of a cabinet door is large, a certain time is needed for selecting a culture dish, and the tightness is poor after the cabinet door is opened and closed in the prior art.

In order to achieve the purpose, the invention provides the following technical scheme: a cell culture device for medical examination, comprising:

a box body;

the four supporting legs are fixedly arranged at the four corners of the bottom end of the box body respectively;

the transparent plate is arranged in the middle of the front side of the box body and can be opened and closed along the vertical direction;

the temperature controller is fixedly arranged at the top end of the front side of the box body;

the sealing mechanism is fixedly arranged at the front side position of the bottom end of the transparent plate;

the base is fixedly arranged at the bottom end of the inner cavity of the box body;

the four support rods are respectively and fixedly arranged at the left end and the right end of the front side and the rear side of the base along the vertical direction;

the turnover mechanism is arranged at the top end of the supporting rod;

and the driving mechanism is arranged in the middle of the right side of the bottom end of the base.

Preferably, the box body is further provided with: the grating plate is fixedly arranged in the middle of the bottom end of the front side of the box body; the first sterilization filter screen is fixedly arranged on the rear side of the grating plate; the protective net is fixedly arranged in a groove in the middle of the top end of the rear side of the box body; the ventilation fan is mounted on the inner side of the inner wall of the rear end of the box body and is positioned right in front of the protective net; and the second sterilization filter screen is fixedly arranged on the inner wall of the inner cavity of the box body and is positioned right in front of the ventilation fan.

Preferably, the sealing mechanism comprises; the handle is fixedly arranged in the middle of the bottom end of the front side of the transparent plate along the vertical direction; the handle can rotate around the axis of the handle and is clamped at the front side of the bottom end of the handle; the screw rod is fixedly arranged in the middle of the rear end of the screwing handle; the inserted bar can move along the front and back direction and is screwed on the outer side of the outer wall of the screw rod; and the clamping block is fixedly arranged at the rear end of the inserted rod.

Preferably, the middle position of the front side of the box body is further provided with a groove in the vertical direction, and the top end of the fixture block and the bottom of the groove are inclined.

Preferably, the turnover mechanism comprises: the supporting frame is fixedly arranged at the top ends of the four supporting rods; the fixing columns are fixedly arranged on the inner walls of the upper end and the lower end of the inner side of the support frame along the left-right direction respectively; the four fixed plates are respectively and fixedly arranged on the inner sides of the fixed columns in the vertical direction, and the fixed plates are semicircular in shape; the chain wheel is arranged on the inner side of the fixing plate and can rotate around the axis of the chain wheel; the two chains are respectively rotatably sleeved outside the two chain wheels which are adjacent up and down; the fixing pieces are sequentially and equidistantly fixedly arranged on the inner side of the chain, and the outer sides of the fixing pieces extend to the outer side of the chain; the first rotating shafts can rotate around the axes of the first rotating shafts and are arranged between the two adjacent left and right fixing pieces; the two connecting blocks are rotatably sleeved above the outer sides of the left end and the right end of the first rotating shaft through bearings; the two connecting rods are respectively and fixedly arranged on the front side and the rear side of the bottom end of the connecting block; the bearing platform is fixedly arranged on the inner sides of the bottom ends of the four connecting rods along the horizontal direction.

Preferably, the distance between two adjacent fixing pieces is greater than the distance between the first rotating shaft and the bearing table.

Preferably, the driving mechanism includes: the two second rotating shafts are respectively and fixedly connected to the central positions of the two adjacent left and right chain wheels along the horizontal direction; the motor is fixedly arranged in the middle of the right side of the bottom end of the base; the first belt pulley is locked at the output end of the left side of the motor through a coupling; the second belt pulley is fixedly sleeved on the outer side of the outer wall of the right side of the second rotating shaft below the second belt pulley; and the connecting belt is sleeved and locked at the middle position of the outer side of the outer walls of the first belt pulley and the second belt pulley.

The cell culture device for medical examination provided by the invention has the beneficial effects that:

1. the invention has the advantages of more perfect structure, higher space utilization rate, smaller taking outlet, reduced contact area with the external environment and ensured cell culture environment;

2. according to the invention, the culture dish to be taken can be moved to the transparent plate in advance through the mutual matching action between the turnover mechanism and the driving mechanism, and then the culture dish is taken, so that the selection time for culturing is effectively shortened, the exposure time of the internal culture dish and the external environment is reduced, and the environment for cell culture is further ensured;

3. the sealing mechanism can seal the opened and closed transparent plate, prevent bacteria from entering the position and is beneficial to culturing cells.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic perspective view of the turnover mechanism of the present invention;

FIG. 3 is a schematic right sectional view of the present invention;

FIG. 4 is a left side sectional structural schematic view of the present invention;

FIG. 5 is a schematic rear perspective view of the present invention;

fig. 6 is an enlarged view of a portion a in fig. 4.

In the figure: 1. the box, 2, the supporting leg, 3, the transparent plate, 4, the temperature controller, 5, sealing mechanism, 6, the base, 7, the bracing piece, 8, tilting mechanism, 9, actuating mechanism, 10, the grid board, 11, first filter screen that disinfects, 12, the protection network, 13, the scavenger fan, 14, second filter screen that disinfects, 51, the handle, 52, twist the hand, 53, the screw rod, 54, the inserted bar, 55, the fixture block, 81, the support frame, 82, the fixed column, 83, the fixed plate, 84, the sprocket, 85, the chain, 86, the stationary blade, 87, first pivot, 88, the connecting block, 89, the connecting rod, 810, the plummer, 91, the second pivot, 92, the motor, 93, first belt pulley, 94, the second belt pulley, 95, the connecting belt.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-6, the present invention provides a technical solution: a cell culture device for medical examination, comprising: a box body 1; the four-support-leg type solar water heater comprises support legs 2, a transparent plate 3, a temperature controller 4, a sealing mechanism 5, a base 6, a support rod 7, a turnover mechanism 8 and a driving mechanism 9, wherein the four support legs 2 are fixedly arranged at four corners of the bottom end of a box body 1 respectively; the stability of the device is ensured, the transparent plate 3 can be opened and closed along the up-down direction and is arranged at the middle position of the front side of the box body 1, and workers can conveniently take culture dishes and observe the internal conditions; the temperature controller 4 is fixedly arranged at the top end of the front side of the box body 1 and controls the internal temperature; the sealing mechanism 5 is fixedly arranged at the front side position of the bottom end of the transparent plate 3; the base 6 is fixedly arranged at the bottom end of the inner cavity of the box body 1; two of the four support rods 7 are respectively fixedly arranged at the left end and the right end of the front side and the rear side of the base 6 along the up-down direction; the turnover mechanism 8 is arranged at the top end of the support rod 7; the driving mechanism 9 is arranged at the middle position of the right side of the bottom end of the base 6.

Preferably, the box 1 is further provided with: the air purifier comprises a grating plate 10, a first sterilization filter screen 11, a protective net 12, an air exchange fan 13 and a second sterilization filter screen 14, wherein the grating plate 10 is fixedly arranged in the middle of the bottom end of the front side of the box body 1; the first sterilizing filter screen 11 is fixedly arranged at the rear side of the grating plate 10; the protective net 12 is fixedly arranged in a groove in the middle of the top end of the rear side of the box body 1; the ventilation fan 13 is arranged on the inner side of the inner wall of the rear end of the box body 1 and is positioned right in front of the protective net 12, so that the air circulation speed in the device is increased; the second sterilization filter screen 14 is fixedly installed on the inner wall of the inner cavity of the box body 1 and is positioned right in front of the ventilation fan 13 to prevent bacteria from entering.

Preferably, the sealing mechanism 5 further comprises; the handle 51, the screw handle 52, the screw 53, the inserted link 54 and the fixture block 55, the handle 51 is fixedly arranged at the middle position of the bottom end of the front side of the transparent plate 3 along the up-down direction; the screw handle 52 can be clamped at the front side of the bottom end of the handle 51 in a rotating way around the axis of the screw handle; the screw 53 is fixedly arranged at the middle position of the rear end of the wrench 52; the insert rod 54 is screwed on the outer side of the outer wall of the screw 53 and can move in the front-rear direction; the latch 55 is fixedly installed at the rear end of the insertion rod 54, and the sealing effect at the transparent plate 3 is ensured by the sealing mechanism 5.

Preferably, a vertical groove is further formed in the middle of the front side of the box 1, and the top end of the latch 55 and the bottom of the groove are both inclined to force the transparent plate 3 to be in close contact with the box.

Preferably, the turnover mechanism 8 further includes: the supporting frame 81, the fixing column 82, the fixing plate 83, the chain wheel 84, the chain 85, the fixing sheet 86, the first rotating shaft 87, the connecting block 88, the connecting rod 89 and the bearing table 810 are fixedly arranged at the top ends of the four supporting rods 7; a plurality of fixing columns 82 are respectively and fixedly arranged on the inner walls of the upper end and the lower end of the inner side of the supporting frame 81 along the left-right direction; the four fixing plates 83 are respectively and fixedly installed on the inner sides of the fixing columns 82 along the vertical direction, and the fixing plates 83 are all semicircular in shape; the chain wheel 84 is arranged on the inner side of the fixed plate 83 and can rotate around the axis of the chain wheel; the two chains 85 are respectively rotatably sleeved outside the two chain wheels 84 adjacent up and down; the plurality of fixing pieces 86 are sequentially and equidistantly fixedly arranged on the inner side of the chain 85, and the outer sides of the fixing pieces extend to the outer side of the chain 85, so that the culture dish is turned over; a plurality of first rotating shafts 87 can be arranged between the two adjacent left and right fixing plates 86 in a rotating way around the axes thereof; the two connecting blocks 88 are rotatably sleeved above the outer sides of the left end and the right end of the first rotating shaft 87 through bearings, so that the first rotating shaft is always kept in a horizontal state; the two connecting rods 89 are respectively fixedly arranged at the front side and the rear side of the bottom end of the connecting block 88, and play a role in hoisting the bearing platform 810; the bearing table 810 is fixedly installed at the inner side of the bottom ends of the four connecting rods 89 along the horizontal direction and is used for bearing a cell culture dish.

Preferably, the distance between two adjacent fixing plates 86 is larger than the distance between the first rotating shaft 87 and the bearing platform 810.

Preferably, the driving mechanism 9 further includes: the two second rotating shafts 91 are respectively and fixedly connected to the center positions of the two adjacent left and right chain wheels 84 along the horizontal direction; the motor 92 is fixedly arranged in the middle of the right side of the bottom end of the base 6 and is used as a main power driving source in the device; the first belt pulley 93 is locked at the output end of the left side of the motor 92 through a coupling; the second belt pulley 94 is fixedly sleeved outside the outer wall on the right side of the second rotating shaft 91 positioned below; the connecting belt 95 is locked in the middle of the outer side of the first pulley 93 and the second pulley 94.

The detailed connection means is a technique known in the art, and the following mainly describes the working principle and process, and the specific operation is as follows.

The method comprises the following steps: in the actual use process of the device, the temperature of the internal space is controlled and set through the temperature controller 4, the ventilation fan 13 is started to accelerate the air circulation to the inside, bacteria in the air can be effectively prevented from entering through the first sterilization filter screen 11 and the second sterilization filter screen 14, and the growth environment of the cell culture dish in the culture state is effectively ensured;

step two, when the cell culture dish needs to be taken out, firstly, the motor 92 is started, because the first belt pulley 93 is locked at the output end of the motor 92 through the coupler, after the motor 92 is started, the first belt pulley 93 is driven to rotate, after the first belt pulley 93 rotates, the second belt pulley 94 and the second rotating shaft 91 positioned below are driven to synchronously rotate under the action of the connecting belt 95, the two chain wheels 84 positioned below are simultaneously driven to rotate, then the two chain wheels 84 positioned above and the second rotating shaft 91 are driven to synchronously rotate through the chain 85, so as to achieve the purpose of driving the turnover mechanism 8, because the plurality of fixing plates 86 are all equidistantly arranged at the inner side of the chain 85, the first rotating shaft 87 can be arranged between the two fixing plates 86 in a rotating way around the axis of the first rotating shaft 87, after the chain 85 rotates, the plurality of first rotating shafts 87 are driven to rotate around the path at the outer side of the chain, the connecting block 88 is rotatably sleeved outside the outer wall of the first rotating shaft 87 through the rotating shaft and plays a role in hoisting the bearing platform 810 below through the connecting rod 89, so that the bearing platform 810 is always kept in a horizontal state and can synchronously rotate according to the rotation of the chain 85, and the switching function of the bearing platform 810 is realized; when the culture dish to be taken moves to the position at the front side of the belt, the culture dish is taken by opening the transparent plate 3;

step three: after taking out the culture dish, it is closed with transparent plate 3, then rotate through the staff and twist hand 52, because screw rod 53 is fixed connection relation with twisting hand 52, after twisting hand 52 is rotatory, also will drive the bolt simultaneously and carry out synchronous revolution, again because inserted bar 54 spiro union is in the outside of screw rod 53, after screw rod 53 rotates, will drive inserted bar 54 and rotate along left right direction, in order to reach the effect in promoting the recess of box 1 front side middle part bottom with fixture block 55, and under the limiting action of fixture block 55 and this recess self shape, force sealing mechanism 5 whole to continue to move down gradually, prevent gap from appearing in transparent plate 3 bottom, guaranteed that culture apparatus takes out the sealed effect of back transparent plate 3 department.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A cell culture device for medical examination, comprising:

a box body (1);

the four supporting legs (2) are respectively and fixedly arranged at the four corners of the bottom end of the box body (1);

the transparent plate (3) can be opened and closed along the vertical direction and is arranged in the middle of the front side of the box body (1);

the temperature controller (4), the said temperature controller (4) is fixedly mounted on the top of front side of the said container body (1);

the sealing mechanism (5) is fixedly arranged at the front side position of the bottom end of the transparent plate (3);

the base (6), the said base (6) is fixedly set up in the bottom end of cavity of the said container body (1);

the four supporting rods (7) are respectively and fixedly arranged at the left end and the right end of the front side and the rear side of the base (6) along the vertical direction in a group of two supporting rods (7);

the turnover mechanism (8), the turnover mechanism (8) is installed at the top end of the support rod (7);

and the driving mechanism (9), wherein the driving mechanism (9) is arranged in the middle position of the right side of the bottom end of the base (6).

2. The cell culture apparatus for medical examination according to claim 1, wherein: the box body (1) is also provided with:

the grating plate (10), the grating plate (10) is fixedly arranged in the middle of the bottom end of the front side of the box body (1);

the first sterilization filter screen (11), the first sterilization filter screen (11) is fixedly arranged at the rear side of the grating plate (10);

the protective net (12) is fixedly arranged in a groove in the middle of the top end of the rear side of the box body (1);

the ventilation fan (13) is mounted on the inner side of the inner wall of the rear end of the box body (1) and is positioned right in front of the protective net (12);

and the second sterilization filter screen (14), the second sterilization filter screen (14) is fixedly arranged on the inner wall of the inner cavity of the box body (1) and is positioned right in front of the ventilation fan (13).

3. The cell culture apparatus for medical examination according to claim 1, wherein: the sealing mechanism (5) comprises;

the handle (51), the said handle (51) is fixedly mounted on the middle position of bottom end of front side of the said transparent plate (3) along the up and down direction;

the screw handle (52) is rotatably clamped on the front side of the bottom end of the handle (51) around the axis of the screw handle (52);

the screw rod (53) is fixedly arranged at the middle position of the rear end of the screwing handle (52);

an insert rod (54), wherein the insert rod (54) can move in the front-back direction and is screwed outside the outer wall of the screw rod (53);

a fixture block (55), wherein the fixture block (55) is fixedly arranged at the rear end of the insert rod (54).

4. The cell culture apparatus for medical examination according to claim 3, wherein: the middle position of the front side of the box body (1) is also provided with a groove in the vertical direction, and the top end of the fixture block (55) and the bottom of the groove are inclined.

5. The cell culture apparatus for medical examination according to claim 1, wherein: the turnover mechanism (8) comprises:

the supporting frames (81), the supporting frames (81) are fixedly arranged at the top ends of the four supporting rods (7);

the fixing columns (82) are respectively and fixedly installed on the inner walls of the upper end and the lower end of the inner side of the supporting frame (81) along the left-right direction;

the four fixing plates (83) are respectively and fixedly arranged on the inner sides of the fixing columns (82) along the vertical direction, and the fixing plates (83) are semicircular in shape;

a sprocket (84), wherein the sprocket (84) is arranged on the inner side of the fixing plate (83) in a manner that the sprocket can rotate around the axis of the sprocket (84);

the two chains (85) are respectively rotatably sleeved on the outer sides of the two chain wheels (84) which are adjacent up and down;

the fixing plates (86) are sequentially and equidistantly fixedly arranged on the inner side of the chain (85), and the outer sides of the fixing plates (86) extend to the outer side of the chain (85);

the first rotating shafts (87), a plurality of the first rotating shafts (87) can be arranged between the two adjacent left and right fixing plates (86) in a rotating mode around the axes of the first rotating shafts (87);

the two connecting blocks (88) are rotatably sleeved above the outer sides of the left end and the right end of the first rotating shaft (87) through bearings;

the two connecting rods (89) are respectively and fixedly arranged on the front side and the rear side of the bottom end of the connecting block (88);

plummer (810), plummer (810) are along horizontal direction fixed mounting four the bottom inboard of connecting rod (89).

6. The cell culture apparatus for medical examination according to claim 5, wherein: the distance between two adjacent fixing plates (86) is larger than the distance between the first rotating shaft (87) and the bearing table (810).

7. The cell culture apparatus for medical examination according to claim 1, wherein: the drive mechanism (9) comprises:

the two second rotating shafts (91) are respectively and fixedly connected to the central positions of the two left and right adjacent chain wheels (84) along the horizontal direction;

the motor (92), the said motor (92) is fixedly mounted in the middle position of right side of bottom end of the said base (6);

the first belt pulley (93), the first belt pulley (93) is locked at the output end of the left side of the motor (92) through a coupling;

the second belt pulley (94), the second belt pulley (94) is fixedly sleeved outside the outer wall of the right side of the second rotating shaft (91) below;

and the connecting belt (95) is sleeved and locked at the middle position of the outer side of the outer walls of the first belt pulley (93) and the second belt pulley (94).