CN110903974A - A constant temperature cell culture container for cell culture for medical experiment - Google Patents

A constant temperature cell culture container for cell culture for medical experiment Download PDF

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CN110903974A
CN110903974A CN201911269962.6A CN201911269962A CN110903974A CN 110903974 A CN110903974 A CN 110903974A CN 201911269962 A CN201911269962 A CN 201911269962A CN 110903974 A CN110903974 A CN 110903974A
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heat
barrel
culture
cell culture
preservation
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苏春英
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04Filters; Permeable or porous membranes or plates, e.g. dialysis
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20Degassing; Venting; Bubble traps
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

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Abstract

The invention provides a constant-temperature cell culture container for cell culture for medical experiments, relates to the technical field of medical cell culture, and solves the problems that the ventilation and heat dissipation effects of the interior of the conventional container are poor, when the culture temperature of the interior of the container is too high, the residual heat in the interior of the container cannot be timely discharged, and the constant-temperature culture effect of the interior of the container is easily influenced. A constant temperature cell culture container for cell culture for medical experiment comprises a heat preservation culture barrel which is in a barrel-shaped structure and is arranged on the top of a base, and a power supply connection jack is arranged on the top of the heat preservation culture barrel and is connected with an external power supply circuit through a power supply plug; the temperature control panel is arranged on the end face of the outer side of the heat-preservation culture barrel. According to the invention, when the temperature in the heat-preservation culture barrel exceeds the proper temperature, the heat-dissipation fan at the bottom of the heat-preservation culture barrel is opened through the temperature control panel, so that the waste heat in the heat-preservation culture barrel is extracted through the exhaust hole, and the rapid cooling operation in the heat-preservation culture barrel is realized.

Description

A constant temperature cell culture container for cell culture for medical experiment
Technical Field
The invention belongs to the technical field of medical cell culture, and particularly relates to a constant-temperature cell culture container for cell culture for medical tests.
Background
The cell culture container is a special laboratory vessel for cell culture, the cell culture is used as an important component of a medical test, and the cell culture container is widely applied to the field of medical disease research and lays a solid foundation for the research and treatment of diseases; the constant-temperature cell culture container can refer to patent No. CN209412231U, and mainly comprises a culture dish main body, wherein a culture dish cover is covered above the culture dish main body, the uppermost surface in the culture dish main body is a culture dish surface layer, and the bottom layer of the culture dish is arranged below the culture dish surface layer; a heat conduction layer is fixed between the culture dish surface layer and the culture dish bottom layer through screws, a heating copper pipe is welded on the culture dish bottom layer, one end of the heating copper pipe is connected with a temperature-sensitive resistor in a welding mode, the other end of the heating copper pipe is connected with a temperature sensor in a welding mode, an LCD display screen is embedded in front of the culture dish bottom layer, a controller and a storage battery are embedded behind the LCD display screen, and the controller is connected with the storage battery, the temperature-sensitive resistor and the temperature sensor through wires; the constant-temperature cell culture container can refer to the patent CN208532802U, and mainly comprises a main body, a cover body, a working chamber and a culture dish, wherein anti-skidding foot pads are arranged at four corners of the bottom end of the main body, an observation window is arranged at the middle position of one end of the main body, a nameplate is arranged on the main body at one side of the observation window, a control panel is fixed on the main body at the other side of the observation window, the top end of the main body is hinged with the cover body, a fixed block is fixed at one side of the cover body, which is far away from an articulated shaft, a fixed buckle mutually matched with the fixed block is hinged at the top end of one side of the main body, a groove is arranged at the middle position of the bottom end of; when present similar constant temperature cell culture container is using to cell culture, the inside ventilation radiating effect of container is relatively poor, when the inside culture temperature of container was too high, can't be timely discharge the inside waste heat of container, influences the inside constant temperature culture effect of container easily to it is relatively poor to the inside regular degerming effect of container, after the container is used for a long time, lead to a large amount of bacterial growing inside the container easily, influence the cleanliness factor of the inside cell culture environment of container.
Disclosure of Invention
In order to solve the technical problems, the invention provides a constant-temperature cell culture container for cell culture for medical experiments, which aims to solve the problems that when the existing similar constant-temperature cell culture container is used for cell culture, the ventilation and heat dissipation effects in the container are poor, when the culture temperature in the container is too high, the residual heat in the container cannot be timely discharged, the constant-temperature culture effect in the container is easily influenced, the regular degerming effect on the interior of the container is poor, and after the container is used for a long time, a large amount of bacteria are easily bred in the container, and the cleanliness of the cell culture environment in the container is influenced.
The invention is used for the purpose and efficacy of the constant temperature cell culture container for cell culture of medical experiment, and is achieved by the following specific technical means:
the invention provides a constant-temperature cell culture container for cell culture for medical tests, which comprises a constant-temperature cell culture container for cell culture for medical tests, and comprises a heat-preservation culture barrel, a temperature control panel, a heating plate, a connecting cover, a heat dissipation fan, a barrel cover, a bearing plate, an elastic clamping belt and a sterilization switch, wherein the temperature control panel is arranged on the heat-preservation culture barrel; the heat-preservation culture barrel is of a cylindrical structure and is arranged at the top of the base, and a power supply connection jack is arranged at the top of the heat-preservation culture barrel and is connected with an external power supply circuit through a power supply plug; the temperature control panel is arranged on the end face of the outer side of the heat-preservation culture barrel and is connected with an external power supply circuit through a power plug; the heating plate is positioned inside the heat-preservation culture barrel; the interior of the connecting cover is hollow, and the connecting cover is arranged at the bottom of the heat-preservation culture barrel; the heat dissipation fan is positioned at the bottom of the heat preservation culture barrel and communicated with the interior of the heat preservation culture barrel through the connecting cover; the barrel cover is buckled at the barrel opening at the top of the heat-preservation culture barrel, a power supply connecting plug is arranged at the bottom of the barrel cover, and the power supply connecting plug is inserted and connected into a power supply connecting jack at the top of the heat-preservation culture barrel; the bearing plate is positioned inside the heat-preservation culture barrel; the elastic clamping belts are divided into three groups at fifteen positions, and the elastic clamping belts are respectively arranged on the end surfaces of the tops of the three bearing plates; the sterilization switch is arranged on the end face of the top of the barrel cover and is connected with the power supply connecting plug through a power line.
Furthermore, the heating plate is of an arc-shaped plate structure, is arranged on the inner wall of the heat-preservation culture barrel in a surrounding manner, and is connected with the temperature control panel through a power line; the temperature control panel also comprises a temperature control probe, and the temperature control probe connected with the temperature control panel is positioned inside the heat-preservation culture barrel.
Furthermore, five exhaust holes are formed in the bottom of the heat-preservation culture barrel, the top of the connecting cover is communicated with the exhaust holes in the bottom of the heat-preservation culture barrel, and the heat dissipation fan is installed in the bottom of the connecting cover.
Furthermore, six air inlets are formed in the center of the barrel cover, the bottoms of the air inlets are communicated with the interior of the heat-preservation culture barrel, and filter screens are arranged in the air inlets.
Furthermore, the bearing plate is of a circular hollow plate-shaped structure and is provided with three positions, the three bearing plates are installed on the end face of the bottom of the barrel cover in a layered mode and are placed inside the heat-preservation culture barrel in a sliding mode through the barrel cover, and the end face of the top of each bearing plate is provided with five storage grooves in a surrounding mode.
Furthermore, the elastic clamping belts are telescopic, five elastic clamping belts are arranged at the top end surface of the bearing plate in a surrounding manner, and the five elastic clamping belts respectively surround the outer sides of five storage grooves formed in the bearing plate.
Furthermore, the ultraviolet germicidal lamp is arranged on the end face of the bottom of the barrel cover, the bottom of the ultraviolet germicidal lamp penetrates through the center of the three bearing plates respectively, and the ultraviolet germicidal lamp is electrically connected with the sterilization switch at the top of the barrel cover.
Compared with the prior art, the invention has the following beneficial effects:
1. the temperature control panel and the heating plate are arranged, so that the temperature inside the heat-preservation culture barrel can be favorably raised through the heating plate, when the temperature inside the heat-preservation culture barrel reaches the proper temperature, the temperature is fed back through the temperature control probe connected with the temperature control panel, the heating plate is controlled by the temperature control panel to stop heating, the constant temperature state is kept inside the heat-preservation culture barrel, and the temperature inside the heat-preservation culture barrel can be more suitable for cell culture; and the setting of cooperation heat dissipation fan and connecting cover is favorable to can be when the inside temperature of heat preservation culture barrel surpasss suitable temperature, feeds back the temperature through the control by temperature change probe that the control by temperature change panel is connected, and the control by temperature change panel is opened heat preservation culture barrel bottom heat dissipation fan, makes the inside waste heat of heat preservation culture barrel take out through the exhaust hole, and to the inside cooling of heat preservation culture barrel, it is too high to avoid the inside temperature of heat preservation culture barrel, influences the cell culture effect.
2. The arrangement of the bearing plate and the elastic clamping belt is beneficial to placing culture dishes storing test cells in a storage groove at the top of the bearing plate in a layered mode, and the culture dishes are fixed through the elastic clamping belt, so that the cell culture dishes are positioned at the bottom of the barrel cover, and therefore a person can insert the cell culture dishes at the top of the bearing plate into the heat-preservation culture barrel through the barrel cover, and the cell culture dishes in the heat-preservation culture barrel can be stored and taken more conveniently; and the use of cooperation ultraviolet germicidal lamp can regularly open the inside ultraviolet germicidal lamp of heat preservation culture bucket, accepts the board through ultraviolet germicidal lamp to the inside each layer of heat preservation culture bucket and disinfects and shine, reduces the inside bacterial quantity of heat preservation culture bucket, the cleanliness factor of the inside cell culture environment of guarantee heat preservation culture bucket.
3. The bearing plate is of a circular hollow plate-shaped structure, and is provided with three positions, the three bearing plates are arranged on the end surface of the bottom of the barrel cover in a layered mode, the bearing plates are placed in the heat-preservation culture barrel in a sliding mode through the barrel cover, and five storage grooves are formed in the end surface of the top of the bearing plates in a surrounding mode; the concrete effect can place the culture dish that has deposited experimental cell respectively in each storage tank in the accept board top, makes the cell culture dish can arrange in the bung bottom by layering to will accept board top cell culture dish through the bung and place in the heat preservation culture bucket, make the inside cell culture dish of heat preservation culture bucket access more convenient change.
4. Still be, the elasticity centre gripping area is scalable form, and accepts board top end and personally submits the form of encircleing and install five elasticity centre gripping areas to five elasticity centre gripping areas encircle respectively in five storage tank outsides that accept the board and offer, can with the culture dish outside that the culture dish placed of elasticity centre gripping area suit in the storage tank inside, fix the culture dish that accepts the board top and place through the elasticity centre gripping area, ensure the stability that the culture dish was deposited.
Drawings
Fig. 1 is a schematic axial view of the present invention.
Fig. 2 is a bottom axial view of the present invention.
Fig. 3 is a schematic view of the split structure of the present invention.
FIG. 4 is a schematic view of the installation structure of the heating plate inside the incubation bucket of the present invention.
FIG. 5 is a schematic sectional view of the incubation bucket of the present invention.
Fig. 6 is a schematic bottom axial view of the heat dissipating fan of the present invention.
Fig. 7 is a schematic view of the connection structure of the barrel cover and the bearing plate of the present invention.
Fig. 8 is a bottom axial view of the invention in fig. 7.
FIG. 9 is a schematic axial view of the adapting plate of the present invention.
In the drawings, the corresponding relationship between the component names and the reference numbers is as follows:
1. a base; 2. a heat-preservation culture barrel; 201. an exhaust hole; 202. a power supply connection jack; 3. a temperature control panel; 301. a temperature control probe; 4. heating plates; 5. a connecting cover; 6. a heat dissipation fan; 7. a barrel cover; 701. an air inlet; 702. a power supply connecting plug; 8. a bearing plate; 801. a storage tank; 9. an elastic clamping band; 10. a sterilization switch; 11. an ultraviolet germicidal lamp.
Detailed Description
The embodiments of the present invention will be described in further detail with reference to the drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "connected" and "connected" are to be interpreted broadly, e.g., as being fixed or detachable or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Example (b):
as shown in figures 1 to 9:
the invention provides a constant-temperature cell culture container for cell culture for medical tests, which comprises a heat-preservation culture barrel 2, a temperature control panel 3, a heating plate 4, a connecting cover 5, a heat dissipation fan 6, a barrel cover 7, a bearing plate 8, an elastic clamping belt 9 and a sterilization switch 10; the heat-preservation culture barrel 2 is of a cylindrical structure, the heat-preservation culture barrel 2 is installed at the top of the base 1, a power supply connection jack 202 is arranged at the top of the heat-preservation culture barrel 2, and the power supply connection jack 202 is connected with an external power supply circuit through a power supply plug; the temperature control panel 3 is arranged on the end face of the outer side of the heat-preservation culture barrel 2, and the temperature control panel 3 is connected with an external power supply circuit through a power plug; the heating plate 4 is positioned inside the heat-preservation culture barrel 2; the interior of the connecting cover 5 is hollow, and the connecting cover 5 is arranged at the bottom of the heat-preservation culture barrel 2; the heat dissipation fan 6 is positioned at the bottom of the heat preservation culture barrel 2, and the heat dissipation fan 6 is communicated with the interior of the heat preservation culture barrel 2 through the connecting cover 5; the barrel cover 7 is buckled at the barrel opening at the top of the heat-preservation culture barrel 2, a power supply connecting plug 702 is arranged at the bottom of the barrel cover 7, and the power supply connecting plug 702 is inserted and connected into the power supply connecting jack 202 at the top of the heat-preservation culture barrel 2; six air inlets 701 are formed in the center of the barrel cover 7, the bottoms of the air inlets 701 are communicated with the interior of the heat-preservation culture barrel 2, a filter screen is arranged in each air inlet 701, and therefore when the heat dissipation fan 6 at the bottom of the heat-preservation culture barrel 2 operates, outside air can be sucked into the heat-preservation culture barrel 2 through the air inlets 701 in the barrel cover 7 to replace hot air in the heat-preservation culture barrel 2, and the air circulation and heat dissipation effects in the heat-preservation culture barrel 2 are guaranteed; the bearing plate 8 is positioned inside the heat-preservation culture barrel 2; fifteen elastic clamping belts 9 are divided into three groups, and the elastic clamping belts 9 are respectively arranged on the top end surfaces of the three bearing plates 8; the sterilizing switch 10 is installed on the top end face of the barrel cover 7, and the sterilizing switch 10 is connected with the power supply connecting plug 702 through a power line.
Wherein, the heating plate 4 is in a circular arc plate-shaped structure, the heating plate 4 is arranged on the inner wall of the heat preservation culture barrel 2 in a surrounding way, and the heating plate 4 is connected with the temperature control panel 3 through a power line; the temperature control panel 3 also comprises a temperature control probe 301, and the temperature control probe 301 connected with the temperature control panel 3 is positioned in the heat-preservation culture barrel 2; the concrete effect, accessible hot plate 4 cultivates 2 insides of bucket to keeping warm and encircles the heating, makes 2 inside arrival suitable temperatures of heat preservation cultivation bucket, and temperature control probe 301 feeds back the temperature, and 3 control heating board 4 stop heating of control by temperature change panel, 2 inside constant temperature states that keep warm cultivation bucket, and it is required that 2 inside temperatures of messenger's heat preservation cultivation bucket can more suitable cell culture.
Wherein, the bottom of the heat-preservation culture barrel 2 is provided with five exhaust holes 201, the top of the connecting cover 5 is communicated with the exhaust holes 201 at the bottom of the heat-preservation culture barrel 2, and the heat dissipation fan 6 is arranged in the bottom of the connecting cover 5; the concrete effect, can link to each other heat dissipation fan 6 with control by temperature change panel 3 through the power cord, can cultivate 2 inside temperatures of bucket in the heat preservation when surpassing suitable temperature, the control by temperature change probe 301 that connects through control by temperature change panel 3 feeds back the temperature, control by temperature change panel 3 is opened heat dissipation fan 6 in 2 bottoms of culture by heat preservation bucket, make 2 inside waste heat of culture by heat preservation bucket take out through exhaust hole 201, cultivate 2 insides in the bucket in the heat preservation and cool down, avoid 2 inside temperatures of culture by heat preservation bucket too high, influence the cell culture effect.
The bearing plate 8 is of a round hollowed plate-shaped structure, and has three positions, the three bearing plates 8 are installed on the end face of the bottom of the barrel cover 7 in a layered manner, the bearing plate 8 is placed inside the heat-preservation culture barrel 2 in a sliding manner through the barrel cover 7, and five storage grooves 801 are formed in the end face of the top of the bearing plate 8 in a surrounding manner; the concrete effect can place the culture dish that has deposited experimental cell respectively in each storage tank 801 in 8 tops of accept board, makes the cell culture dish can the layering list in 7 bottoms of bung to insert 8 tops of accept board cell culture dish in heat preservation culture bucket 2 through bung 7, make 2 inside cell culture dishes of heat preservation culture bucket access more convenient change.
The elastic clamping belts 9 are telescopic, five elastic clamping belts 9 are arranged on the top end face of the bearing plate 8 in a surrounding manner, and the five elastic clamping belts 9 surround the outer sides of five storage grooves 801 formed in the bearing plate 8 respectively; the concrete effect can be with the culture dish outside that the inside placing of storage tank 801 was taken over to the suit of elasticity centre gripping area 9, fixes the culture dish that the top of accepting board 8 was placed through elasticity centre gripping area 9, the stability that the guarantee culture dish was deposited.
The ultraviolet sterilizing lamp 11 is arranged on the end face of the bottom of the barrel cover 7, the bottom of the ultraviolet sterilizing lamp 11 penetrates through the center parts of the three bearing plates 8 respectively, and the ultraviolet sterilizing lamp 11 is electrically connected with the sterilizing switch 10 on the top of the barrel cover 7; specific effect can regularly open ultraviolet germicidal lamp 11 through 7 top degerming switches 10 of bung, disinfects to 2 inside irradiation of cultivateing of heat preservation bucket, reduces 2 inside bacterium quantity of heat preservation cultivation bucket, makes 2 inside more clean purifications of heat preservation cultivation bucket, and the inside cell culture effect of guarantee heat preservation cultivation bucket 2.
The specific use mode and function of the embodiment are as follows:
when the cell culture plate is used, a worker firstly pulls a handle at the top of the barrel cover 7 to take out the bearing plate 8 in the heat-preservation culture barrel 2, places a culture dish with cells in the storage groove 801 at the top of the bearing plate 8 and fixes the cell culture dish through the elastic clamping belt 9 surrounding the outer side of the storage groove 801; next, the worker places the cell culture dishes arranged on the top of the bearing plate 8 inside the heat-preservation culture barrel 2 through the barrel cover 7, the heating plate 4 inside the heat-preservation culture barrel 2 is started through the temperature control panel 3, and the heating plate 4 circularly heats the inside of the heat-preservation culture barrel 2; when the interior of the heat-preservation culture barrel 2 reaches the appropriate temperature, the temperature is fed back through the temperature control probe 301 connected with the temperature control panel 3, the temperature control panel 3 controls the heating plate 4 to stop heating, and the interior of the heat-preservation culture barrel 2 is kept in a constant temperature state; when the temperature in the heat-preservation culture barrel 2 exceeds the proper temperature, the temperature is fed back through the temperature control probe 301 connected with the temperature control panel 3, the temperature control panel 3 opens the heat dissipation fan 6 at the bottom of the heat-preservation culture barrel 2, so that the waste heat in the heat-preservation culture barrel 2 is exhausted through the exhaust hole 201, the outside air enters the heat-preservation culture barrel 2 through the air inlet hole 701 formed in the barrel cover 7, the temperature in the heat-preservation culture barrel 2 is reduced, and the phenomenon that the temperature in the heat-preservation culture barrel 2 is too high to influence the cell culture effect is avoided; after 2 use a period of time of heat preservation culture barrel, the staff can press 7 top sterilization switch 10 of bung, open 7 bottom ultraviolet germicidal lamp 11 of bung, disinfect through ultraviolet germicidal lamp 11 to 2 insides of heat preservation culture barrel and shine, reduce 2 inside bacterial count of heat preservation culture barrel, the cleanliness factor of the inside cell culture environment of guarantee heat preservation culture barrel 2.
The embodiments of the present invention have been presented for purposes of illustration and description, and are not intended to be exhaustive or limited to the invention in the form disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiment was chosen and described in order to best explain the principles of the invention and the practical application, and to enable others of ordinary skill in the art to understand the invention for various embodiments with various modifications as are suited to the particular use contemplated.

Claims (7)

1. A constant-temperature cell culture container for cell culture for medical tests, characterized in that: comprises a heat-preservation culture barrel (2), a temperature control panel (3), a heating plate (4), a connecting cover (5), a heat dissipation fan (6), a barrel cover (7), a bearing plate (8), an elastic clamping belt (9) and a sterilization switch (10); the heat-preservation culture barrel (2) is of a barrel-shaped structure, the heat-preservation culture barrel (2) is installed at the top of the base (1), a power supply connection jack (202) is arranged at the top of the heat-preservation culture barrel (2), and the power supply connection jack (202) is connected with an external power supply circuit through a power supply plug; the temperature control panel (3) is arranged on the end face of the outer side of the heat-preservation culture barrel (2), and the temperature control panel (3) is connected with an external power supply circuit through a power plug; the heating plate (4) is positioned inside the heat-preservation culture barrel (2); the interior of the connecting cover (5) is hollow, and the connecting cover (5) is arranged at the bottom of the heat-preservation culture barrel (2); the heat dissipation fan (6) is positioned at the bottom of the heat preservation culture barrel (2), and the heat dissipation fan (6) is communicated with the interior of the heat preservation culture barrel (2) through the connecting cover (5); the barrel cover (7) is buckled at the barrel opening at the top of the heat preservation culture barrel (2), a power supply connecting plug (702) is arranged at the bottom of the barrel cover (7), and the power supply connecting plug (702) is inserted and connected into the power supply connecting jack (202) at the top of the heat preservation culture barrel (2); the bearing plate (8) is positioned inside the heat-preservation culture barrel (2); the elastic clamping belts (9) are divided into three groups of fifteen parts in total, and the elastic clamping belts (9) are respectively arranged on the top end surfaces of the three bearing plates (8); the sterilizing switch (10) is arranged on the top end face of the barrel cover (7), and the sterilizing switch (10) is connected with the power supply connecting plug (702) through a power line.
2. A thermostatic cell culture vessel for cell culture for medical test according to claim 1, characterized in that: the heating plate (4) is of an arc-shaped plate structure, the heating plate (4) is arranged on the inner wall of the heat-preservation culture barrel (2) in a surrounding manner, and the heating plate (4) is connected with the temperature control panel (3) through a power line; the temperature control panel (3) further comprises a temperature control probe (301), and the temperature control probe (301) connected with the temperature control panel (3) is positioned inside the heat preservation culture barrel (2).
3. A thermostatic cell culture vessel for cell culture for medical test according to claim 1, characterized in that: five exhaust holes (201) are formed in the bottom of the heat-preservation culture barrel (2), the top of the connecting cover (5) is communicated with the exhaust holes (201) in the bottom of the heat-preservation culture barrel (2), and the heat dissipation fan (6) is installed in the bottom of the connecting cover (5).
4. A thermostatic cell culture vessel for cell culture for medical test according to claim 1, characterized in that: six air inlets (701) are formed in the center of the barrel cover (7), the bottoms of the air inlets (701) are communicated with the inside of the heat-preservation culture barrel (2), and a filter screen is arranged inside the air inlets (701).
5. A thermostatic cell culture vessel for cell culture for medical test according to claim 1, characterized in that: the bearing plate (8) is of a circular hollowed-out plate-shaped structure and is provided with three positions, the bearing plates (8) at the three positions are installed on the end face of the bottom of the barrel cover (7) in a layered mode, the bearing plates (8) are placed inside the heat-preservation culture barrel (2) in a sliding mode through the barrel cover (7), and the end face of the top of the bearing plates (8) is provided with five storage tanks (801) in a surrounding mode.
6. A thermostatic cell culture vessel for cell culture for medical test according to claim 1, characterized in that: the elastic clamping belts (9) are telescopic, the top end faces of the bearing plates (8) are provided with five elastic clamping belts (9) in a surrounding manner, and the five elastic clamping belts (9) respectively surround the outer sides of five storage grooves (801) formed in the bearing plates (8).
7. A thermostatic cell culture vessel for cell culture for medical test according to claim 1, characterized in that: the ultraviolet sterilizing lamp (11) is arranged on the end face of the bottom of the barrel cover (7), the bottom of the ultraviolet sterilizing lamp (11) penetrates through the center of the three bearing plates (8) respectively, and the ultraviolet sterilizing lamp (11) is electrically connected with the sterilizing switch 10 on the top of the barrel cover (7).
CN201911269962.6A 2019-12-11 2019-12-11 A constant temperature cell culture container for cell culture for medical experiment Withdrawn CN110903974A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111909826A (en) * 2020-08-07 2020-11-10 三门颜临医疗器械有限公司 Ventilation circulation system of species incubator
CN112501018A (en) * 2020-11-19 2021-03-16 孙敏 Cell culture device for medical examination

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111909826A (en) * 2020-08-07 2020-11-10 三门颜临医疗器械有限公司 Ventilation circulation system of species incubator
CN112501018A (en) * 2020-11-19 2021-03-16 孙敏 Cell culture device for medical examination

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Application publication date: 20200324